MD02G1315700.v1.1

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006948
Physical Location & Seq
Forward (+)
37036184 .. 37038564
2381 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1315700.v1.1.491

Sequence Viewer

Length: 1290 bp
ATGAGCGGTCTTCTATACAAATACAATCCTAATTTTAAGGCCTCGGAGGGAGAGCCGAGGAAGAACCACGCGGCGGAGTTCATGGATTCGAATATTTTCCACCAGCAGCATCCTCAGCAGCAGCAGAGCTCCGGCTTAACGCGGTATCAGTCAGCTCCAAACTCATTTCTGATGGAGCAGATGGACAACAATGGCGGCGGAACCCAGGATTTGAGGTATCTTCAACCTTCGAGCCCTGAAGTCGAAACGGTGCTGGCCAGGTTCATTTCTTCATGTAATGAACCAGATCAACGTGACAATGGTGTGCAGCAGCATCAGTTTGAAATCCAAGAGAGGCCGGTAAATGTGAAGGGAGAGGCAGGGGACTCTGTTTCTGAGCACATTAATGGTTACTCGAATTCTACTCATATGATGTACCAAGCTCCTCAAGGTCAGCAAGCTCATGGTTTAGACAGCAGCTCCTTTGCTGGAGTCAACTCTACGGGAATGGAGAATTCAATGATGCAATTGAAAATTGGGGTCGGAAATCGGTCTAATCATGTTAGACAAAGTAGCTCTCCGGCTGGTGTCTTCCCAAACTCGACCGTTGACGATGGCTTTAACGTGATGAAGGACTCGGCAGGATATCGCGCAGGCAATGGTACAAATGGAGAAGCCAGTCCATCAACTTCTAGGTTTGGTAATCAACTGAGCTTCTCATCAAGGCAATCTTCATACTCAGGTCGAATGCCTCGGATTGCTGAAGATGAGAATGGGAACTTGGGAGAAGGTAGCCAATCGCAACCAGATCATAGTTTAGGAAATGCTAATGGTAGCAATTCACCTTACCAGTCTAGCTTCCCAGATGATTCGTTGGACGATTCTTCATTTAATGACCTCAAAAGAGCCAGAGACAATGATGGGAACAAGTTTTCTACTTCGACTGCATTTGAATCACAGAACAATGATTTTCGACACCGCAATCTTGGCTTGACGCAACACTTGAGGTTTCCCAACCATTTTGAGATGCCTGCTATGGAGAAGTATTTGCAATTTGAAGATTCTATTCCTCGTAAAATTCGTGCCAAAAGAGGTTTCGCCACTCACCCGCGAAGCATTGCAGAGAGGATGAGAAGGACGCGGATTAGTGAAAGAATGAAGAAATTGCAGGAGCTTTTCCCAAACATGGACAAGCAAACAAACACGGCAGAAAAGTTGGACTTGGCCGTCGGGTTCATAAAAGACCTTCAGAAACAGGTTCAGACACTCAAGGATACGAAGGCCAAGTGCAGTTGTTCGAGCGAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

430

Amino Acids

47.84

Weight (kDa)

6.51

Isoelectric Point (pI)

52.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 365 - 410 2.3e-06 Helix-loop-helix DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1205
AccBSI CCGCTC 1 cut(s) 6
AccII CGCG 5 cut(s) 71, 142, 630, 1090, 1120
AciI CCGC 9 cut(s) 6, 71, 74, 142, 195, 198, 958, 1088, 1120
AcoI YGGCCR 2 cut(s) 255, 1203
AcsI RAATTY 3 cut(s) 397, 493, 1056
AcuI CTGAAG 3 cut(s) 258, 762, 1211
AfaI GTAC 2 cut(s) 416, 643
AfiI CCNNNNNNNGG 2 cut(s) 73, 1165
AgsI TTSAA 6 cut(s) 224, 323, 498, 511, 932, 1037
AjnI CCWGG 2 cut(s) 204, 257
AjuI GAANNNNNNNTTGG 4 cut(s) 743, 775, 1058, 1090
AluBI AGCT 9 cut(s) 129, 155, 422, 440, 459, 555, 693, 837, 1153
AluI AGCT 9 cut(s) 129, 155, 422, 440, 459, 555, 693, 837, 1153
Alw21I GWGCWC 2 cut(s) 131, 381
Alw26I GTCTC 1 cut(s) 885
AoxI GGCC 5 cut(s) 39, 255, 335, 1203, 1260
ApeKI GCWGC 6 cut(s) 106, 118, 121, 307, 310, 456
ApoI RAATTY 3 cut(s) 397, 493, 1056
AseI ATTAAT 1 cut(s) 384
Asp700I GAANNNNTTC 1 cut(s) 95
AspLEI GCGC 1 cut(s) 632
AsuHPI GGTGA 2 cut(s) 813, 1076
AsuII TTCGAA 1 cut(s) 89
BalI TGGCCA 1 cut(s) 257
BanII GRGCYC 2 cut(s) 131, 236
BbsI GAAGAC 2 cut(s) 2, 562
Bbv12I GWGCWC 2 cut(s) 131, 381
BbvCI CCTCAGC 1 cut(s) 114
BbvI GCAGC 6 cut(s) 118, 130, 133, 319, 322, 468
BccI CCATC 5 cut(s) 166, 175, 587, 670, 893
BceAI ACGGC 2 cut(s) 1190, 1200
BciT130I CCWGG 2 cut(s) 206, 259
BciVI GTATCC 1 cut(s) 1246
BcoDI GTCTC 1 cut(s) 885
BfaI CTAG 2 cut(s) 672, 834
BfuI GTATCC 1 cut(s) 1246
BisI GCNGC 8 cut(s) 72, 107, 119, 122, 196, 308, 311, 457
BlsI GCNGC 8 cut(s) 73, 108, 120, 123, 197, 309, 312, 458
Bme1390I CCNGG 2 cut(s) 206, 259
BmiI GGNNCC 1 cut(s) 202
BmrFI CCNGG 2 cut(s) 206, 259
BmsI GCATC 4 cut(s) 118, 322, 492, 996
BpiI GAAGAC 2 cut(s) 2, 562
BpmI CTGGAG 1 cut(s) 489
Bpu10I CCTNAGC 1 cut(s) 114
Bpu14I TTCGAA 1 cut(s) 89
BpuEI CTTGAG 3 cut(s) 411, 1003, 1232
BsaJI CCNNGG 4 cut(s) 42, 56, 204, 731
BsaXI ACNNNNNCTCC 2 cut(s) 443, 473
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 1165
Bse118I RCCGGY 1 cut(s) 337
Bse1I ACTGG 2 cut(s) 657, 829
Bse3DI GCAATG 2 cut(s) 643, 1095
BseBI CCWGG 2 cut(s) 206, 259
BseDI CCNNGG 4 cut(s) 42, 56, 204, 731
BseGI GGATG 2 cut(s) 109, 1113
BseLI CCNNNNNNNGG 2 cut(s) 73, 1165
BseMI GCAATG 2 cut(s) 643, 1095
BseMII CTCAG 4 cut(s) 128, 366, 680, 732
BseNI ACTGG 2 cut(s) 657, 829
BseRI GAGGAG 1 cut(s) 414
BseXI GCAGC 6 cut(s) 118, 130, 133, 319, 322, 468
BsgI GTGCAG 2 cut(s) 326, 1288
Bsh1236I CGCG 5 cut(s) 71, 142, 630, 1090, 1120
Bsh1285I CGRYCG 1 cut(s) 585
BshFI GGCC 5 cut(s) 41, 257, 337, 1205, 1262
BsiEI CGRYCG 1 cut(s) 585
BsiHKAI GWGCWC 2 cut(s) 131, 381
BsiSI CCGG 3 cut(s) 132, 338, 560
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 2 cut(s) 73, 1165
BsmAI GTCTC 1 cut(s) 885
BsmFI GGGAC 1 cut(s) 377
BsmI GAATGC 1 cut(s) 732
BsnI GGCC 5 cut(s) 41, 257, 337, 1205, 1262
Bsp119I TTCGAA 1 cut(s) 89
Bsp1286I GDGCHC 3 cut(s) 131, 236, 381
Bsp143I GATC 2 cut(s) 286, 787
BspACI CCGC 9 cut(s) 6, 71, 74, 142, 195, 198, 958, 1088, 1120
BspANI GGCC 5 cut(s) 41, 257, 337, 1205, 1262
BspCNI CTCAG 4 cut(s) 127, 367, 681, 731
BspFNI CGCG 5 cut(s) 71, 142, 630, 1090, 1120
BspLI GGNNCC 1 cut(s) 202
BspT104I TTCGAA 1 cut(s) 89
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 2 cut(s) 643, 1095
BsrFI RCCGGY 1 cut(s) 337
BsrI ACTGG 2 cut(s) 657, 829
BssAI RCCGGY 1 cut(s) 337
BssECI CCNNGG 4 cut(s) 42, 56, 204, 731
BssMI GATC 2 cut(s) 286, 787
Bst2UI CCWGG 2 cut(s) 206, 259
Bst4CI ACNGT 2 cut(s) 250, 586
BstBI TTCGAA 1 cut(s) 89
BstC8I GCNNGC 4 cut(s) 255, 438, 634, 1011
BstDEI CTNAG 4 cut(s) 114, 375, 689, 718
BstF5I GGATG 2 cut(s) 109, 1113
BstFNI CGCG 5 cut(s) 71, 142, 630, 1090, 1120
BstHHI GCGC 1 cut(s) 632
BstKTI GATC 2 cut(s) 289, 790
BstMAI GTCTC 1 cut(s) 885
BstMBI GATC 2 cut(s) 286, 787
BstMCI CGRYCG 1 cut(s) 585
BstMWI GCNNNNNNNGC 2 cut(s) 115, 966
BstNI CCWGG 2 cut(s) 206, 259
BstSCI CCNGG 2 cut(s) 204, 257
BstUI CGCG 5 cut(s) 71, 142, 630, 1090, 1120
BstV1I GCAGC 6 cut(s) 118, 130, 133, 319, 322, 468
BstV2I GAAGAC 2 cut(s) 2, 562
BsuI GTATCC 1 cut(s) 1246
BsuRI GGCC 5 cut(s) 41, 257, 337, 1205, 1262
BtsCI GGATG 2 cut(s) 109, 1113
Cac8I GCNNGC 4 cut(s) 255, 438, 634, 1011
CfoI GCGC 1 cut(s) 632
Cfr10I RCCGGY 1 cut(s) 337
CseI GACGC 2 cut(s) 982, 1126
Csp6I GTAC 2 cut(s) 415, 642
CviAII CATG 5 cut(s) 82, 273, 443, 539, 1165
CviQI GTAC 2 cut(s) 415, 642
DdeI CTNAG 4 cut(s) 114, 375, 689, 718
DpnI GATC 2 cut(s) 288, 789
DpnII GATC 2 cut(s) 286, 787
DrdI GACNNNNNNGTC 1 cut(s) 1205
DseDI GACNNNNNNGTC 1 cut(s) 1205
EaeI YGGCCR 2 cut(s) 255, 1203
EciI GGCGGA 2 cut(s) 89, 213
Ecl136II GAGCTC 1 cut(s) 129
Eco147I AGGCCT 1 cut(s) 41
Eco24I GRGCYC 2 cut(s) 131, 236
Eco32I GATATC 1 cut(s) 626
Eco53kI GAGCTC 1 cut(s) 129
Eco57I CTGAAG 3 cut(s) 258, 762, 1211
EcoICRI GAGCTC 1 cut(s) 129
EcoRI GAATTC 2 cut(s) 397, 493
EcoRII CCWGG 2 cut(s) 204, 257
EcoRV GATATC 1 cut(s) 626
EcoT38I GRGCYC 2 cut(s) 131, 236
FaeI CATG 5 cut(s) 85, 276, 446, 542, 1168
FalI AAGNNNNNCTT 4 cut(s) 694, 726, 1184, 1216
FaqI GGGAC 1 cut(s) 377
FatI CATG 5 cut(s) 81, 272, 442, 538, 1164
FauI CCCGC 1 cut(s) 1095
FauNDI CATATG 1 cut(s) 408
Fnu4HI GCNGC 8 cut(s) 72, 107, 119, 122, 196, 308, 311, 457
FokI GGATG 2 cut(s) 96, 1120
FriOI GRGCYC 2 cut(s) 131, 236
Fsp4HI GCNGC 8 cut(s) 72, 107, 119, 122, 196, 308, 311, 457
FspBI CTAG 2 cut(s) 672, 834
GlaI GCGC 1 cut(s) 631
GluI GCNGC 8 cut(s) 72, 107, 119, 122, 196, 308, 311, 457
GsuI CTGGAG 1 cut(s) 489
HaeIII GGCC 5 cut(s) 41, 257, 337, 1205, 1262
HapII CCGG 3 cut(s) 132, 338, 560
HgaI GACGC 2 cut(s) 982, 1126
HhaI GCGC 1 cut(s) 632
Hin1II CATG 5 cut(s) 85, 276, 446, 542, 1168
Hin6I GCGC 1 cut(s) 630
HinP1I GCGC 1 cut(s) 630
HincII GTYRAC 2 cut(s) 475, 589
HindII GTYRAC 2 cut(s) 475, 589
HinfI GANTC 8 cut(s) 86, 365, 471, 614, 848, 860, 932, 1040
HpaII CCGG 3 cut(s) 132, 338, 560
HphI GGTGA 2 cut(s) 813, 1076
Hpy166II GTNNAC 2 cut(s) 475, 589
Hpy188I TCNGA 7 cut(s) 46, 171, 376, 524, 735, 1230, 1242
Hpy8I GTNNAC 2 cut(s) 475, 589
Hpy99I CGWCG 1 cut(s) 1211
HpyAV CCTTC 7 cut(s) 237, 343, 604, 761, 1107, 1235, 1252
HpyCH4III ACNGT 2 cut(s) 250, 586
HpyCH4IV ACGT 2 cut(s) 292, 603
HpyCH4V TGCA 7 cut(s) 307, 505, 926, 1030, 1100, 1147, 1269
HpyF10VI GCNNNNNNNGC 2 cut(s) 115, 966
HpyF3I CTNAG 4 cut(s) 114, 375, 689, 718
HpySE526I ACGT 2 cut(s) 292, 603
Hsp92II CATG 5 cut(s) 85, 276, 446, 542, 1168
HspAI GCGC 1 cut(s) 630
Kzo9I GATC 2 cut(s) 286, 787
LmnI GCTCC 6 cut(s) 134, 160, 175, 427, 464, 1150
Lsp1109I GCAGC 6 cut(s) 118, 130, 133, 319, 322, 468
LweI GCATC 4 cut(s) 118, 322, 492, 996
MaeI CTAG 2 cut(s) 672, 834
MaeII ACGT 2 cut(s) 292, 603
MaeIII GTNAC 2 cut(s) 293, 389
MalI GATC 2 cut(s) 288, 789
MbiI CCGCTC 1 cut(s) 6
MboI GATC 2 cut(s) 286, 787
MboII GAAGA 9 cut(s) 73, 212, 261, 562, 702, 755, 855, 1049, 1150
MfeI CAATTG 1 cut(s) 506
MhlI GDGCHC 3 cut(s) 131, 236, 381
MlsI TGGCCA 1 cut(s) 257
MluCI AATT 9 cut(s) 31, 397, 493, 506, 513, 817, 1031, 1056, 1142
MluNI TGGCCA 1 cut(s) 257
MlyI GAGTC 3 cut(s) 359, 480, 608
MmeI TCCRAC 3 cut(s) 502, 834, 1176
Mox20I TGGCCA 1 cut(s) 257
MroXI GAANNNNTTC 1 cut(s) 95
MscI TGGCCA 1 cut(s) 257
MseI TTAA 5 cut(s) 36, 137, 384, 600, 870
MslI CAYNNNNRTG 1 cut(s) 384
Msp20I TGGCCA 1 cut(s) 257
MspI CCGG 3 cut(s) 132, 338, 560
MspR9I CCNGG 2 cut(s) 206, 259
MunI CAATTG 1 cut(s) 506
Mva1269I GAATGC 1 cut(s) 732
MvaI CCWGG 2 cut(s) 206, 259
MvnI CGCG 5 cut(s) 71, 142, 630, 1090, 1120
MwoI GCNNNNNNNGC 2 cut(s) 115, 966
NdeI CATATG 1 cut(s) 408
NdeII GATC 2 cut(s) 286, 787
NlaIII CATG 5 cut(s) 85, 276, 446, 542, 1168
NlaIV GGNNCC 1 cut(s) 202
NmeAIII GCCGAG 2 cut(s) 81, 596
NmuCI GTSAC 1 cut(s) 293
NspV TTCGAA 1 cut(s) 89
PceI AGGCCT 1 cut(s) 41
PcsI WCGNNNNNNNCGW 1 cut(s) 1057
PctI GAATGC 1 cut(s) 732
PdmI GAANNNNTTC 1 cut(s) 95
PfeI GAWTC 5 cut(s) 86, 848, 860, 932, 1040
PkrI GCNGC 8 cut(s) 73, 108, 120, 123, 197, 309, 312, 458
PleI GAGTC 3 cut(s) 359, 479, 608
PpsI GAGTC 3 cut(s) 359, 479, 608
PshBI ATTAAT 1 cut(s) 384
Psp124BI GAGCTC 1 cut(s) 131
Psp6I CCWGG 2 cut(s) 204, 257
PspGI CCWGG 2 cut(s) 204, 257
PspN4I GGNNCC 1 cut(s) 202
RsaI GTAC 2 cut(s) 416, 643
RsaNI GTAC 2 cut(s) 415, 642
RseI CAYNNNNRTG 1 cut(s) 384
SacI GAGCTC 1 cut(s) 131
SaqAI TTAA 5 cut(s) 36, 137, 384, 600, 870
SatI GCNGC 8 cut(s) 72, 107, 119, 122, 196, 308, 311, 457
Sau3AI GATC 2 cut(s) 286, 787
SchI GAGTC 3 cut(s) 359, 480, 608
ScrFI CCNGG 2 cut(s) 206, 259
SduI GDGCHC 3 cut(s) 131, 236, 381
SfaNI GCATC 4 cut(s) 118, 322, 492, 996
SfuI TTCGAA 1 cut(s) 89
SmiMI CAYNNNNRTG 1 cut(s) 384
SmlI CTYRAG 3 cut(s) 426, 982, 1247
SmoI CTYRAG 3 cut(s) 426, 982, 1247
Sse9I AATT 9 cut(s) 31, 397, 493, 506, 513, 817, 1031, 1056, 1142
SseBI AGGCCT 1 cut(s) 41
SsiI CCGC 9 cut(s) 6, 71, 74, 142, 195, 198, 958, 1088, 1120
SspI AATATT 1 cut(s) 94
SspMI CTAG 2 cut(s) 672, 834
SstI GAGCTC 1 cut(s) 131
StuI AGGCCT 1 cut(s) 41
StyD4I CCNGG 2 cut(s) 204, 257
TaaI ACNGT 2 cut(s) 250, 586
TaiI ACGT 2 cut(s) 295, 606
TaqI TCGA 9 cut(s) 89, 230, 243, 395, 581, 724, 920, 952, 1277
TaqII GACCGA 1 cut(s) 519
TasI AATT 9 cut(s) 31, 397, 493, 506, 513, 817, 1031, 1056, 1142
TauI GCSGC 2 cut(s) 74, 198
TfiI GAWTC 5 cut(s) 86, 848, 860, 932, 1040
Tru1I TTAA 5 cut(s) 36, 137, 384, 600, 870
Tru9I TTAA 5 cut(s) 36, 137, 384, 600, 870
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 6 cut(s) 106, 118, 121, 307, 310, 456
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 9 cut(s) 70, 253, 261, 294, 623, 702, 855, 1151, 1204
VspI ATTAAT 1 cut(s) 384
XapI RAATTY 3 cut(s) 397, 493, 1056
XmnI GAANNNNTTC 1 cut(s) 95
XspI CTAG 2 cut(s) 672, 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.