pycom02g26450

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0006948
Physical Location & Seq
Forward (+)
24227418 .. 24229259
1842 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g26450.3

Sequence Viewer

Length: 1290 bp
ATGAGCAGTCTTCTGTACAAATACAATCCTAATTTTAAGGCCTCGGAGGGAGAGCTGAGGAAGAACCACGCGGCGGAGTTCATGGATTCGGATATTTTCCACCACCAGCATCCTCAGCAGCAGCAGAGCTCCGGCTTAACGCGGTATCAGTCAGCTCCAAGCTCATTTCTGATGGAGCAGATGGACAACAATGGCGGCGGAACTGAGGATTTGCGGTATCTTCAACCTTTGAGCCCTGAAGTCGAAACGGTGCTGCCCAGGTTCATTTCTTCATGTAACGAACCAGATCAACGTGACAATGGTGTGCAGCAGCATCAGTTTGAAATCCAAGAGAGGCCGGTAAATGTGAAGGGAGAGGCAGGGGACTCTGTTTCTGAGCACATTAATGGTTACTCGAATTCTACTCATATGACGTACCAAGCTCCTCGAGGTCAGCAAGCTCATGGTTTAGAAAGCAGCTCCTTTGCTGCAGTCAACTCTACGGGAATGGAGAATTCAATGATGCAATCGAAAATTGGGGTCGGAAATCGGTCTAATATTGTTAGACAAAGTAGCTCTCCGGCTGGATTCTTCCCAGACTTGACCGTTGACGATGGCTTTAACGTGATGAAGGACTCGGCAGGATATCGCGCAGGCAATGGTACAAATGGAGAAGCCAGTCCATCAACTTCTAGGTTTGGTACTCAACTGAGTTTCTCATCAAGGCCATCTTCATACTCAGGTCGAATGCCTCGGATTGCTGAAAATGAGAATGGGAACTTGGGAGAAGGTAGCCAATCGCAACCGGATCATAGTTTAGGAAATGCTAATGGTAGCAATTCACCTTACCAGTCTAGCTTCGCAAATGATTCGTGGGACGATTCTTCATTTAATGACCTCAAAATAGCCGGAGACAATGATGGGAACAAGTTTTCTACTTCGACTGCATTTGAATCACAGAACAATGATTTTGGACACCGCAATCATGGCTTGACGCAACACTTGAGGTTTTCCAAACATTTTGAGATGCCTGCTATGGAGAAGTATTTGCAATTTGAAGATTCTACTCCTTGTAAAAGTCGTGCCAAAAGAGGCTTCGCCACTCACCCGCGAAGCATTGCAGAGAGGATGAGAAGGACGCGGATTAGTGAAAGAATGAAGAAATTGCAGGAGCTTTTCCCAGACATGGACAAGCAAACAAACACGGCAGAAATGTTGGACTTGGCGGCCGGGTTCATAAAGGACCTTCAGAAACAGGTTAAGACACTCAAGGATTCGAAGGCGAAGTGCAGTTGTTCGAGCAAACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

430

Amino Acids

47.63

Weight (kDa)

6.28

Isoelectric Point (pI)

51.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 426
AccII CGCG 5 cut(s) 71, 142, 630, 1090, 1120
AclWI GGATC 1 cut(s) 795
AcoI YGGCCR 1 cut(s) 1206
AcsI RAATTY 2 cut(s) 397, 493
AcuI CTGAAG 2 cut(s) 258, 1211
AfaI GTAC 4 cut(s) 17, 416, 643, 682
AfiI CCNNNNNNNGG 2 cut(s) 73, 1165
AgsI TTSAA 5 cut(s) 224, 323, 498, 932, 1037
AjnI CCWGG 1 cut(s) 257
AjuI GAANNNNNNNTTGG 4 cut(s) 743, 775, 1058, 1090
Alw21I GWGCWC 2 cut(s) 131, 381
Alw26I GTCTC 1 cut(s) 885
AlwI GGATC 1 cut(s) 795
Ama87I CYCGRG 1 cut(s) 426
AoxI GGCC 4 cut(s) 39, 335, 704, 1206
ApeKI GCWGC 7 cut(s) 118, 121, 253, 307, 310, 456, 467
ApoI RAATTY 2 cut(s) 397, 493
AseI ATTAAT 1 cut(s) 384
AspLEI GCGC 1 cut(s) 632
AspS9I GGNCC 1 cut(s) 1222
AsuC2I CCSGG 1 cut(s) 1210
AsuHPI GGTGA 2 cut(s) 813, 1076
AsuII TTCGAA 1 cut(s) 1256
AvaI CYCGRG 1 cut(s) 426
AvaII GGWCC 1 cut(s) 1222
BanII GRGCYC 2 cut(s) 131, 236
BbsI GAAGAC 1 cut(s) 2
Bbv12I GWGCWC 2 cut(s) 131, 381
BbvCI CCTCAGC 2 cut(s) 56, 114
BbvI GCAGC 7 cut(s) 130, 133, 240, 319, 322, 454, 468
BccI CCATC 6 cut(s) 166, 175, 587, 670, 715, 893
BceAI ACGGC 1 cut(s) 1200
BcgI CGANNNNNNTGC 2 cut(s) 831, 865
BciT130I CCWGG 1 cut(s) 259
BcnI CCSGG 1 cut(s) 1210
BcoDI GTCTC 1 cut(s) 885
BfaI CTAG 2 cut(s) 672, 834
BfmI CTRYAG 1 cut(s) 468
Bme1390I CCNGG 2 cut(s) 259, 1210
Bme18I GGWCC 1 cut(s) 1222
BmeT110I CYCGRG 1 cut(s) 426
BmgT120I GGNCC 1 cut(s) 1222
BmrFI CCNGG 2 cut(s) 259, 1210
BmsI GCATC 4 cut(s) 118, 322, 492, 996
BpiI GAAGAC 1 cut(s) 2
Bpu10I CCTNAGC 2 cut(s) 56, 114
Bpu14I TTCGAA 1 cut(s) 1256
BpuEI CTTGAG 2 cut(s) 1003, 1232
BpuMI CCSGG 1 cut(s) 1210
BsaJI CCNNGG 3 cut(s) 42, 257, 731
BsaWI WCCGGW 1 cut(s) 784
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 1165
Bse118I RCCGGY 1 cut(s) 337
Bse1I ACTGG 2 cut(s) 657, 829
Bse3DI GCAATG 2 cut(s) 643, 1095
BseBI CCWGG 1 cut(s) 259
BseDI CCNNGG 3 cut(s) 42, 257, 731
BseGI GGATG 2 cut(s) 109, 1113
BseLI CCNNNNNNNGG 2 cut(s) 73, 1165
BseMI GCAATG 2 cut(s) 643, 1095
BseMII CTCAG 6 cut(s) 47, 128, 195, 366, 680, 732
BseNI ACTGG 2 cut(s) 657, 829
BseRI GAGGAG 1 cut(s) 414
BseX3I CGGCCG 1 cut(s) 1206
BseXI GCAGC 7 cut(s) 130, 133, 240, 319, 322, 454, 468
BsgI GTGCAG 2 cut(s) 326, 1288
Bsh1236I CGCG 5 cut(s) 71, 142, 630, 1090, 1120
Bsh1285I CGRYCG 1 cut(s) 1209
BshFI GGCC 4 cut(s) 41, 337, 706, 1208
BsiEI CGRYCG 1 cut(s) 1209
BsiHKAI GWGCWC 2 cut(s) 131, 381
BsiHKCI CYCGRG 1 cut(s) 426
BsiSI CCGG 6 cut(s) 132, 338, 560, 785, 888, 1209
BslFI GGGAC 2 cut(s) 377, 869
BslI CCNNNNNNNGG 2 cut(s) 73, 1165
BsmAI GTCTC 1 cut(s) 885
BsmFI GGGAC 2 cut(s) 377, 869
BsmI GAATGC 1 cut(s) 732
BsnI GGCC 4 cut(s) 41, 337, 706, 1208
BsoBI CYCGRG 1 cut(s) 426
Bsp119I TTCGAA 1 cut(s) 1256
Bsp1286I GDGCHC 3 cut(s) 131, 236, 381
Bsp1407I TGTACA 1 cut(s) 15
Bsp143I GATC 2 cut(s) 286, 787
BspANI GGCC 4 cut(s) 41, 337, 706, 1208
BspCNI CTCAG 6 cut(s) 48, 127, 196, 367, 681, 731
BspFNI CGCG 5 cut(s) 71, 142, 630, 1090, 1120
BspMAI CTGCAG 1 cut(s) 472
BspPI GGATC 1 cut(s) 795
BspT104I TTCGAA 1 cut(s) 1256
BsrDI GCAATG 2 cut(s) 643, 1095
BsrFI RCCGGY 1 cut(s) 337
BsrGI TGTACA 1 cut(s) 15
BsrI ACTGG 2 cut(s) 657, 829
BssAI RCCGGY 1 cut(s) 337
BssECI CCNNGG 3 cut(s) 42, 257, 731
BssMI GATC 2 cut(s) 286, 787
Bst2UI CCWGG 1 cut(s) 259
Bst4CI ACNGT 2 cut(s) 250, 586
BstAUI TGTACA 1 cut(s) 15
BstBI TTCGAA 1 cut(s) 1256
BstC8I GCNNGC 3 cut(s) 438, 634, 1011
BstDEI CTNAG 6 cut(s) 56, 114, 204, 375, 689, 718
BstF5I GGATG 2 cut(s) 109, 1113
BstFNI CGCG 5 cut(s) 71, 142, 630, 1090, 1120
BstHHI GCGC 1 cut(s) 632
BstKTI GATC 2 cut(s) 289, 790
BstMAI GTCTC 1 cut(s) 885
BstMBI GATC 2 cut(s) 286, 787
BstMCI CGRYCG 1 cut(s) 1209
BstMWI GCNNNNNNNGC 2 cut(s) 115, 966
BstNI CCWGG 1 cut(s) 259
BstSCI CCNGG 2 cut(s) 257, 1208
BstSFI CTRYAG 1 cut(s) 468
BstUI CGCG 5 cut(s) 71, 142, 630, 1090, 1120
BstV1I GCAGC 7 cut(s) 130, 133, 240, 319, 322, 454, 468
BstV2I GAAGAC 1 cut(s) 2
BstZI CGGCCG 1 cut(s) 1206
BsuRI GGCC 4 cut(s) 41, 337, 706, 1208
BtsCI GGATG 2 cut(s) 109, 1113
Cac8I GCNNGC 3 cut(s) 438, 634, 1011
CfoI GCGC 1 cut(s) 632
Cfr10I RCCGGY 1 cut(s) 337
Cfr13I GGNCC 1 cut(s) 1222
CseI GACGC 2 cut(s) 982, 1126
Csp6I GTAC 4 cut(s) 16, 415, 642, 681
CviAII CATG 5 cut(s) 82, 273, 443, 965, 1165
CviQI GTAC 4 cut(s) 16, 415, 642, 681
DdeI CTNAG 6 cut(s) 56, 114, 204, 375, 689, 718
DpnI GATC 2 cut(s) 288, 789
DpnII GATC 2 cut(s) 286, 787
EaeI YGGCCR 1 cut(s) 1206
EagI CGGCCG 1 cut(s) 1206
EciI GGCGGA 2 cut(s) 89, 213
Ecl136II GAGCTC 1 cut(s) 129
EclXI CGGCCG 1 cut(s) 1206
Eco147I AGGCCT 1 cut(s) 41
Eco24I GRGCYC 2 cut(s) 131, 236
Eco32I GATATC 1 cut(s) 626
Eco47I GGWCC 1 cut(s) 1222
Eco52I CGGCCG 1 cut(s) 1206
Eco53kI GAGCTC 1 cut(s) 129
Eco57I CTGAAG 2 cut(s) 258, 1211
Eco88I CYCGRG 1 cut(s) 426
EcoICRI GAGCTC 1 cut(s) 129
EcoO109I RGGNCCY 1 cut(s) 1222
EcoRI GAATTC 2 cut(s) 397, 493
EcoRII CCWGG 1 cut(s) 257
EcoRV GATATC 1 cut(s) 626
EcoT38I GRGCYC 2 cut(s) 131, 236
FaeI CATG 5 cut(s) 85, 276, 446, 968, 1168
FalI AAGNNNNNCTT 2 cut(s) 694, 726
FaqI GGGAC 2 cut(s) 377, 869
FatI CATG 5 cut(s) 81, 272, 442, 964, 1164
FauI CCCGC 1 cut(s) 1095
FauNDI CATATG 1 cut(s) 408
FokI GGATG 2 cut(s) 96, 1120
FriOI GRGCYC 2 cut(s) 131, 236
FspBI CTAG 2 cut(s) 672, 834
GlaI GCGC 1 cut(s) 631
HaeIII GGCC 4 cut(s) 41, 337, 706, 1208
HapII CCGG 6 cut(s) 132, 338, 560, 785, 888, 1209
HgaI GACGC 2 cut(s) 982, 1126
HhaI GCGC 1 cut(s) 632
Hin1II CATG 5 cut(s) 85, 276, 446, 968, 1168
Hin6I GCGC 1 cut(s) 630
HinP1I GCGC 1 cut(s) 630
HincII GTYRAC 2 cut(s) 475, 589
HindII GTYRAC 2 cut(s) 475, 589
HinfI GANTC 9 cut(s) 86, 365, 567, 614, 848, 860, 932, 1040, 1253
HpaII CCGG 6 cut(s) 132, 338, 560, 785, 888, 1209
HphI GGTGA 2 cut(s) 813, 1076
Hpy166II GTNNAC 2 cut(s) 475, 589
Hpy188I TCNGA 7 cut(s) 46, 91, 171, 376, 524, 735, 1230
Hpy8I GTNNAC 2 cut(s) 475, 589
HpyAV CCTTC 6 cut(s) 343, 604, 761, 1107, 1235, 1252
HpyCH4III ACNGT 2 cut(s) 250, 586
HpyCH4IV ACGT 3 cut(s) 292, 413, 603
HpyCH4V TGCA 8 cut(s) 307, 470, 505, 926, 1030, 1100, 1147, 1269
HpyF10VI GCNNNNNNNGC 2 cut(s) 115, 966
HpyF3I CTNAG 6 cut(s) 56, 114, 204, 375, 689, 718
HpySE526I ACGT 3 cut(s) 292, 413, 603
Hsp92II CATG 5 cut(s) 85, 276, 446, 968, 1168
HspAI GCGC 1 cut(s) 630
Kzo9I GATC 2 cut(s) 286, 787
LmnI GCTCC 6 cut(s) 134, 160, 175, 427, 464, 1150
Lsp1109I GCAGC 7 cut(s) 130, 133, 240, 319, 322, 454, 468
LweI GCATC 4 cut(s) 118, 322, 492, 996
MaeI CTAG 2 cut(s) 672, 834
MaeII ACGT 3 cut(s) 292, 413, 603
MaeIII GTNAC 3 cut(s) 275, 293, 389
MalI GATC 2 cut(s) 288, 789
MboI GATC 2 cut(s) 286, 787
MboII GAAGA 8 cut(s) 73, 212, 261, 562, 702, 855, 1049, 1150
MhlI GDGCHC 3 cut(s) 131, 236, 381
MluCI AATT 7 cut(s) 31, 397, 493, 513, 817, 1031, 1142
MlyI GAGTC 2 cut(s) 359, 608
MmeI TCCRAC 2 cut(s) 502, 1176
MseI TTAA 6 cut(s) 36, 137, 384, 600, 870, 1239
MslI CAYNNNNRTG 1 cut(s) 384
MspI CCGG 6 cut(s) 132, 338, 560, 785, 888, 1209
MspR9I CCNGG 2 cut(s) 259, 1210
Mva1269I GAATGC 1 cut(s) 732
MvaI CCWGG 1 cut(s) 259
MvnI CGCG 5 cut(s) 71, 142, 630, 1090, 1120
MwoI GCNNNNNNNGC 2 cut(s) 115, 966
NciI CCSGG 1 cut(s) 1210
NdeI CATATG 1 cut(s) 408
NdeII GATC 2 cut(s) 286, 787
NlaIII CATG 5 cut(s) 85, 276, 446, 968, 1168
NmeAIII GCCGAG 1 cut(s) 596
NmuCI GTSAC 1 cut(s) 293
NspV TTCGAA 1 cut(s) 1256
PaeR7I CTCGAG 1 cut(s) 426
PceI AGGCCT 1 cut(s) 41
PctI GAATGC 1 cut(s) 732
PfeI GAWTC 7 cut(s) 86, 567, 848, 860, 932, 1040, 1253
PleI GAGTC 2 cut(s) 359, 608
PpsI GAGTC 2 cut(s) 359, 608
PpuMI RGGWCCY 1 cut(s) 1222
PshBI ATTAAT 1 cut(s) 384
Psp124BI GAGCTC 1 cut(s) 131
Psp5II RGGWCCY 1 cut(s) 1222
Psp6I CCWGG 1 cut(s) 257
PspGI CCWGG 1 cut(s) 257
PspPI GGNCC 1 cut(s) 1222
PspPPI RGGWCCY 1 cut(s) 1222
PspXI VCTCGAGB 1 cut(s) 426
PstI CTGCAG 1 cut(s) 472
RsaI GTAC 4 cut(s) 17, 416, 643, 682
RsaNI GTAC 4 cut(s) 16, 415, 642, 681
RseI CAYNNNNRTG 1 cut(s) 384
SacI GAGCTC 1 cut(s) 131
SaqAI TTAA 6 cut(s) 36, 137, 384, 600, 870, 1239
Sau3AI GATC 2 cut(s) 286, 787
Sau96I GGNCC 1 cut(s) 1222
SchI GAGTC 2 cut(s) 359, 608
ScrFI CCNGG 2 cut(s) 259, 1210
SduI GDGCHC 3 cut(s) 131, 236, 381
SfaNI GCATC 4 cut(s) 118, 322, 492, 996
SfcI CTRYAG 1 cut(s) 468
Sfr274I CTCGAG 1 cut(s) 426
SfuI TTCGAA 1 cut(s) 1256
SinI GGWCC 1 cut(s) 1222
SlaI CTCGAG 1 cut(s) 426
SmiMI CAYNNNNRTG 1 cut(s) 384
SmlI CTYRAG 3 cut(s) 426, 982, 1247
SmoI CTYRAG 3 cut(s) 426, 982, 1247
Sse9I AATT 7 cut(s) 31, 397, 493, 513, 817, 1031, 1142
SseBI AGGCCT 1 cut(s) 41
SspI AATATT 1 cut(s) 538
SspMI CTAG 2 cut(s) 672, 834
SstI GAGCTC 1 cut(s) 131
StuI AGGCCT 1 cut(s) 41
StyD4I CCNGG 2 cut(s) 257, 1208
TaaI ACNGT 2 cut(s) 250, 586
TaiI ACGT 3 cut(s) 295, 416, 606
TaqI TCGA 8 cut(s) 243, 395, 427, 509, 724, 920, 1256, 1277
TaqII GACCGA 1 cut(s) 519
TasI AATT 7 cut(s) 31, 397, 493, 513, 817, 1031, 1142
TatI WGTACW 1 cut(s) 15
TauI GCSGC 3 cut(s) 74, 198, 1208
TfiI GAWTC 7 cut(s) 86, 567, 848, 860, 932, 1040, 1253
Tru1I TTAA 6 cut(s) 36, 137, 384, 600, 870, 1239
Tru9I TTAA 6 cut(s) 36, 137, 384, 600, 870, 1239
TseFI GTSAC 1 cut(s) 293
TseI GCWGC 7 cut(s) 118, 121, 253, 307, 310, 456, 467
Tsp45I GTSAC 1 cut(s) 293
TspDTI ATGAA 8 cut(s) 70, 253, 261, 623, 702, 855, 1151, 1204
VpaK11BI GGWCC 1 cut(s) 1222
VspI ATTAAT 1 cut(s) 384
XapI RAATTY 2 cut(s) 397, 493
XhoI CTCGAG 1 cut(s) 426
XspI CTAG 2 cut(s) 672, 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.