MD03G1000900.v1.1

TATA-binding protein-associated factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007901
Physical Location & Seq
Forward (+)
45239 .. 61226
15988 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1000900.v1.1.491

Sequence Viewer

Length: 4254 bp
ATGTTGCTTTGGGATATATTACTTGATTTGGATGATTTAAGTCCATCCACTAGCAGCGTAATGAATCTTTTGGCGGAAATCTATTCCCAGGAAGACATGATTCCTAAGATTTTTGAGGCATTGACATCACAAGAGATTGATTTGAATGAATTGGGTAGCATTGATGATACGGGAGAAGGATTAATTTCGCAAGATAATCCTTTCATGTTATCAACACTTGCACCGCGATTGTGGCCCTTTATGAGGCATAGTATCACATCAGTTCGTTATTCAGCTATCCGCACGTTGGAACGACTACTTGAAGCAGGATACAAAAGGAGTCTCTCTGAGTCTTCGAGTACTTCATTTTGGCCTTCTTTTATCTTGGGTGATACCCTTCGGATTGTTTTTCAGAATTTACTGCTTGAATCAAATGACGAAATTTTGAAACGTTCAGAGAGAGTTTGGGGGCTCCTTGTTCAGTGTCCATTAGGTGACTTGGAAATTGCTGCAAGGTCATATATGTCCTCTTGGATAGGACTTGCAACTACTCCGTATGGATCAGCATTAGATTCTACAAAAATGTTTTGGCCTGTTGCTCTCCCACGAAAGAGTCATTTTAAAGCAGCAGCTAAAATGAGAGCTGTGAATCTTGTAAATGAGTCTTGTAGAAACATTGGGTTGGAATCTTCGAAGGGATCCATTCCGGAGGAAAAAGGTGGAGATGCTTCAACCAATAATGTTCAGATAGTTGTTGGTGCGGATGTTGAAATGTCTGTTATGCGTACCAGAGTGGTTACTGCAGCTGCATTGGGAGTTTTTGCTTCTAGATTGCATGAAGGTTCAATGCAGTATGTAATCGATCCACTGACTAAAGCATTTACCTCTTTATCTGGTGTCCAGCGGCAGGTGGCATCTATGGTTCTTCTTTCTTGGTTCAAAGAGATTAAAAGTGCAGATGTGTTTGAAAACGCTGGAGTTATGCCAGGTTTTCTGAACCATCTCAAACATTTGATGTTGGATTTCTTGGCATGCTCTGAACCTGGTTTCCCTACAAACGATCCTTGTCTTCCTTATGCTGAGCTTTCAAGAACGTATTGTAAGATGCGTTGTGAGGCTAGTCAGTTGTTAAATGCTATTCAATCATCGGGTATGTTTCAGAGTTTGTTGTCTACTACCAAAATTGACTTGGAAAGGTTGAACGTGGACAATGCAATTAATTTTGCATCAAAACTTCCAATGTTGTGTAATGATATTGGGGGAAATGATTCTTTGGAAAGGCACATTGTTGACGATATAGAGTCAGCAAAACGACGACTTTTGACAACTTGTGGATATTTAAAATGTGTTCAGAGCAATCTGCATGTTACTGTCTCTTCTCTTGTTGCTGCTTCTGTTGTTTGGATGTCAGAGCTCCCTGAACGACTAAATCCAATTATTCTGCCTTTGATGGCTTCTATCAAAAGAGAACAGGAGGAAATACTACAAGAGAAGGCAGCTGAGGCACTTGCAGAGCTTATTTCTCATTGTATTTCACGTAGCCGAAGCCCAAATGATAAGTTAATTAAGAATATATGTAATTTAACATGTGTGGATCCTAGAGAAACACCTCAAGCTGCAGTCATCTGTTCCATAGATATTATTGATGACCAAGATCTCCTATCTTTTGGGACAAATACTGGTAAGCAGAAATCAAAGGTCATTCTGGCTGGTAACGAAGACCGCTCAAAGGTTGAAGGCTTTATTAGCAGACGAGGCTCTGAACTTGCCTTGAGGCATCTTTGTGTGAGGTTTGGTGCTTCATTGTTTGATAAGCTTCCAAAACTATGGGATTGCCTCACTGAAGTGCTTAAGCCTAGTAGTATTGAGCCCCTTAATCCTGCAGATGAAAAGAAAATTACACAAACTCTAGAATCTGTGAAGGATCCCCAGATCTTGATAAATAATATCCAGGTAGTACGTTCTATTGCTCCAATGCTAAATGAGGATCTGAAACCAAAACTACTCACCCTTCTCCCATACATATTTAAGTGTGTTCACCATTCCCATGCTGCTGTTAGATTGGCTTCCTCTAGATGCATCACCACAATGGCCAAGTCAATGGAAATGCATGTGATGGGAGCTGTAATTGAAAATGTCATACCCATGTTAGGGGATATGACATCTGTTGATGCCAGACAAGGTGCAGGCATGCTTATTAGTTTGCTTGTCCAAGGACTGGGTGTGGAGCTGGTTCCATATGCTCCTTTCTTAGTTGTCCCTCTTCTAAGGTGTATGAGTGATTGTGATCAGTCTGTCAGACAGAGTGTGACTCGCAGCTTTGCTGCCCTTGTACCTCTTCTTCCACTCACACGGGGCCTCCCTCCACCTGTTGGACTAACTGAAGGTTTCTCCAGAAGTGCAGAAGATGCAACATTTCTGGAGCAACTACTTGACAACTCCCATATTGATGATTACAAGCTTTGTACTGAACTGAAAGTGACACTGAGAAGGTATCAACAGGAAGGCATAAATTGGTTAGCCTTTTTAAAACGTTTCAAGCTTCATGGAATCTTATGTGATGATATGGGTCTTGGTAAGACACTTCAAGCATCTGCTATTGTGGCTTCTGATATAGTTGAGCATCGAACTTTGAATGACGGCAATCCTCCATCATCTTTAATTATTTGCCCATCAACACTTGTTGGACATTGGGCCTTTGAGATAGAGAAGTACATTGATCTTTCCATAATATCTACACTCCAATATGTTGGTTCTGCTCAAGAGCGCATTTCTCTTCGAGAACATTTTGAAAAGCATAATGTGATAATAACATCATATGATGTGGTTCGCAAAGATATTGATTATCTTGGGAAGCTTCTCTGGAACTATTGTATCTTAGATGAAGGACATGTGATCAAGAATGCAAAGTCGAAAATTACACTTTCAGTGAAGCAATTGAAAGCCCAGCATCGTTTGATATTGAGTGGAACACCAATCCAGAATAACGTCATGGATTTGTGGTCCCTTTTTGATTTCCTAATGCCCGGCTTTCTTGGAACAGAGAGGCAGTTCCAAGCTGCTTATGGAAAACCACTGGTGGCAGCCAGAGATCCTAAGTGCTCTGCCAAGGATGCTGAAGCAGGTGCACTTGCGATGGAAGCATTGCACAAGCAGGTCATGCCTTTCCTCCTCCGTCGAACAAAAGATGAAGTCTTGTCTGATCTTCCCGAGAAAATTATTCAGGACAGGTTCTGTGACCTGAGCCCTGTACAATTGAAATTGTATGAACAGTTTTCTGGGTCACATGTGAGACAAGAGATCTCAAGTATTGTAAAACAAAATGAGTCAGCAGATACAGGAGGGCGTAGTGAATCACCTAGAGCTTCATCCCATGTTTTTCAGGCACTTCAGTACTTGCTTAAACTTTGCAGTCACCCATTGCTTGTTCTTGGAGAAAAGGTCCCTGATTCAATTGCATGCCTGTTGTCAGAGCTACTACCGGGAGGCTCTGATACAGTTTCAGAATTGCACAAACCATACCACTCTCCCAAACTAGTTGCTCTTCAGGAGATTCTTGAAGAGTGTGGAATAGGTGTTGATGCTTCTAGTTCTGAAGGTGCCATAAGTGTGGGGCAGCACAGAGTCTTGATATTTGCTCAACATAAAGCATTTCTGGACTTAATAGAAAGAGACTTGTTTCTCACTCATATGAAGAGTGTAACCTACTTGCGGCTGGATGGGTCAGTTGAACCTGAAAAACGTTTTGATATTGTCAAAGCTTTCAATTCGGATCCTACAATCGATGTTCTGCTACTCACAACCCATGTTGGTGGGCTGGGCTTGAACCTGACATCTGCAGACACACTCGTTTTCATGGAGCATGACTGGAACCCCATGCGGGACCATCAGGCAATGGACAGAGCACACAGATTAGGTCAGAAAAAAGTTGTCAATGTCCATCGCCTTATTATGCGTGGCACTCTCGAAGAGAAAGTTATGAGCTTGCAAAAGTTTAAGCTTTCAGTAGCTAATGCAGTCATTAATGCTGAAAATGCTAGTATGAAAACCATGAACACAGACCAACTGCTTGATCTATTTGCTACTGCAGAAACATCTAAAAAGGGAACTTCTATATTAAAGCATCCTGATGGAAAATTTGATGGAGATATGAAATTAATGGGCACTGGAAAGGGGTTGAAAGCAATTCTTGGTGGGCTGGAGGAGTTGTGGGATCAATCACAGTACACCGAAGAATACAATCTCAGTAACTTCTTGGCAAAGCTTGATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0048509 GO:0048518 GO:0050789 GO:0050793 GO:0051094 GO:0065007 GO:1902183 GO:1902185
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3535 HEAT HEAT_EZ Helicase_C SNF2_N

Protein Analysis

1418

Amino Acids

157.29

Weight (kDa)

5.92

Isoelectric Point (pI)

44.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3535 PF12054 148 - 605 1e-98 Domain of unknown function (DUF3535)
HEAT_EF3_N PF24987 624 - 762 5.2e-06 EF3 N-terminal HEAT repeat
SNF2-rel_dom PF00176 825 - 1133 3.7e-70 SNF2-related domain
Helicase_C PF00271 1199 - 1299 1.6e-18 Helicase conserved C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 877, 3092
Acc36I ACCTGC 3 cut(s) 877, 3092, 3124
AccB1I GGYRCC 1 cut(s) 3578
AccB7I CCANNNNNTGG 2 cut(s) 2197, 2351
AccBSI CCGCTC 1 cut(s) 1704
AccI GTMKAC 1 cut(s) 1151
AccII CGCG 1 cut(s) 226
AccIII TCCGGA 1 cut(s) 685
AciI CCGC 8 cut(s) 74, 224, 280, 740, 883, 1702, 3691, 3859
AclI AACGTT 3 cut(s) 430, 2512, 3721
AcoI YGGCCR 1 cut(s) 2070
AcsI RAATTY 3 cut(s) 394, 420, 4115
AcuI CTGAAG 6 cut(s) 1842, 2382, 3117, 3353, 3509, 3594
AfaI GTAC 9 cut(s) 340, 766, 1938, 2313, 2446, 2693, 3231, 3374, 4205
AflII CTTAAG 1 cut(s) 1829
AflIII ACRYGT 3 cut(s) 1565, 2869, 3265
AhlI ACTAGT 1 cut(s) 3514
AjnI CCWGG 4 cut(s) 87, 964, 1021, 1929
AleI CACNNNNGTG 1 cut(s) 1823
AloI GAACNNNNNNTCC 2 cut(s) 1011, 1043
Alw21I GWGCWC 4 cut(s) 1395, 3083, 3109, 3886
Alw26I GTCTC 4 cut(s) 326, 1357, 3265, 3645
Alw44I GTGCAC 1 cut(s) 3105
AlwNI CAGNNNCTG 1 cut(s) 3213
Ama87I CYCGRG 1 cut(s) 3188
Aor13HI TCCGGA 1 cut(s) 685
AoxI GGCC 6 cut(s) 233, 350, 569, 2070, 2335, 2673
ApaLI GTGCAC 1 cut(s) 3105
ApoI RAATTY 3 cut(s) 394, 420, 4115
AseI ATTAAT 4 cut(s) 182, 1197, 4002, 4136
Asp700I GAANNNNTTC 1 cut(s) 1246
AspLEI GCGC 1 cut(s) 2748
AspS9I GGNCC 6 cut(s) 234, 2335, 2673, 2982, 3421, 3862
AsuC2I CCSGG 2 cut(s) 3006, 3462
AsuHPI GGTGA 7 cut(s) 380, 485, 1978, 2009, 2053, 3327, 3386
AsuII TTCGAA 1 cut(s) 671
AvaI CYCGRG 1 cut(s) 3188
AvaII GGWCC 3 cut(s) 2982, 3421, 3862
BaeGI GKGCMC 2 cut(s) 3109, 4145
BaeI ACNNNNGTAYC 2 cut(s) 2836, 2869
BalI TGGCCA 1 cut(s) 2072
BamHI GGATCC 4 cut(s) 677, 1573, 1903, 3751
BanI GGYRCC 1 cut(s) 3578
BanII GRGCYC 4 cut(s) 453, 1395, 1851, 3227
BarI GAAGNNNNNNTAC 2 cut(s) 2304, 2336
BbsI GAAGAC 4 cut(s) 99, 324, 1040, 1704
Bbv12I GWGCWC 4 cut(s) 1395, 3083, 3109, 3886
BbvCI CCTCAGC 1 cut(s) 1479
BceAI ACGGC 1 cut(s) 2635
BciT130I CCWGG 4 cut(s) 89, 966, 1023, 1931
BciVI GTATCC 1 cut(s) 302
BclI TGATCA 2 cut(s) 2266, 2874
BcnI CCSGG 2 cut(s) 3006, 3462
BcoDI GTCTC 4 cut(s) 326, 1357, 3265, 3645
BcuI ACTAGT 1 cut(s) 3514
BfmI CTRYAG 5 cut(s) 780, 1596, 1860, 3816, 4065
BfrI CTTAAG 1 cut(s) 1829
BfuAI ACCTGC 3 cut(s) 877, 3092, 3124
BfuI GTATCC 1 cut(s) 302
BglII AGATCT 3 cut(s) 1633, 1911, 3279
BlpI GCTNAGC 1 cut(s) 1059
BmcAI AGTACT 2 cut(s) 340, 3374
Bme1390I CCNGG 6 cut(s) 89, 966, 1023, 1931, 3006, 3462
Bme18I GGWCC 3 cut(s) 2982, 3421, 3862
BmeT110I CYCGRG 1 cut(s) 3188
BmgT120I GGNCC 6 cut(s) 234, 2335, 2673, 2982, 3421, 3862
BmrFI CCNGG 6 cut(s) 89, 966, 1023, 1931, 3006, 3462
BmrI ACTGGG 1 cut(s) 2207
BmuI ACTGGG 1 cut(s) 2207
BpiI GAAGAC 4 cut(s) 99, 324, 1040, 1704
BplI GAGNNNNNCTC 2 cut(s) 2275, 2307
BpmI CTGGAG 4 cut(s) 975, 2356, 2420, 4199
Bpu10I CCTNAGC 2 cut(s) 1479, 3221
Bpu1102I GCTNAGC 1 cut(s) 1059
Bpu14I TTCGAA 1 cut(s) 671
BpuEI CTTGAG 4 cut(s) 1575, 1771, 2724, 3268
BpuMI CCSGG 2 cut(s) 3006, 3462
Bsa29I ATCGAT 2 cut(s) 840, 3762
BsaAI YACGTR 1 cut(s) 1517
BsaBI GATNNNNATC 1 cut(s) 2265
BsaJI CCNNGG 3 cut(s) 87, 2191, 3087
BsaWI WCCGGW 1 cut(s) 685
BsaXI ACNNNNNCTCC 6 cut(s) 2322, 2352, 3405, 3435, 4172, 4202
Bse1I ACTGG 5 cut(s) 1663, 2202, 3060, 3851, 4150
Bse3DI GCAATG 3 cut(s) 3122, 3398, 3879
Bse8I GATNNNNATC 1 cut(s) 2265
BseAI TCCGGA 1 cut(s) 685
BseBI CCWGG 4 cut(s) 89, 966, 1023, 1931
BseCI ATCGAT 2 cut(s) 840, 3762
BseDI CCNNGG 3 cut(s) 87, 2191, 3087
BseGI GGATG 8 cut(s) 37, 44, 748, 1389, 3097, 3347, 3703, 4102
BseJI GATNNNNATC 1 cut(s) 2265
BseMI GCAATG 3 cut(s) 3122, 3398, 3879
BseMII CTCAG 6 cut(s) 318, 1050, 1470, 2456, 3212, 4237
BseNI ACTGG 5 cut(s) 1663, 2202, 3060, 3851, 4150
BseRI GAGGAG 2 cut(s) 3140, 4196
BseSI GKGCMC 2 cut(s) 3109, 4145
BseYI CCCAGC 2 cut(s) 2925, 3796
BsgI GTGCAG 3 cut(s) 954, 2184, 2400
Bsh1236I CGCG 1 cut(s) 226
BshFI GGCC 6 cut(s) 235, 352, 571, 2072, 2337, 2675
BshNI GGYRCC 1 cut(s) 3578
BshVI ATCGAT 2 cut(s) 840, 3762
BsiHKAI GWGCWC 4 cut(s) 1395, 3083, 3109, 3886
BsiHKCI CYCGRG 1 cut(s) 3188
BsiSI CCGG 3 cut(s) 686, 3006, 3461
BslFI GGGAC 5 cut(s) 1663, 2222, 2968, 3407, 3875
BsmAI GTCTC 4 cut(s) 326, 1357, 3265, 3645
BsmFI GGGAC 5 cut(s) 1663, 2222, 2968, 3407, 3875
BsmI GAATGC 1 cut(s) 2887
BsnI GGCC 6 cut(s) 235, 352, 571, 2072, 2337, 2675
BsoBI CYCGRG 1 cut(s) 3188
Bsp119I TTCGAA 1 cut(s) 671
Bsp1286I GDGCHC 8 cut(s) 453, 1395, 1851, 3083, 3109, 3227, 3886, 4145
Bsp13I TCCGGA 1 cut(s) 685
Bsp1407I TGTACA 1 cut(s) 3229
Bsp1720I GCTNAGC 1 cut(s) 1059
BspACI CCGC 8 cut(s) 74, 224, 280, 740, 883, 1702, 3691, 3859
BspANI GGCC 6 cut(s) 235, 352, 571, 2072, 2337, 2675
BspCNI CTCAG 6 cut(s) 319, 1051, 1471, 2457, 3213, 4236
BspDI ATCGAT 2 cut(s) 840, 3762
BspEI TCCGGA 1 cut(s) 685
BspFNI CGCG 1 cut(s) 226
BspMAI CTGCAG 5 cut(s) 784, 1600, 1864, 3820, 4069
BspMI ACCTGC 3 cut(s) 877, 3092, 3124
BspQI GCTCTTC 1 cut(s) 3528
BspT104I TTCGAA 1 cut(s) 671
BspT107I GGYRCC 1 cut(s) 3578
BspTI CTTAAG 1 cut(s) 1829
BsrBI CCGCTC 1 cut(s) 1704
BsrDI GCAATG 3 cut(s) 3122, 3398, 3879
BsrGI TGTACA 1 cut(s) 3229
BsrI ACTGG 5 cut(s) 1663, 2202, 3060, 3851, 4150
BssECI CCNNGG 3 cut(s) 87, 2191, 3087
BssT1I CCWWGG 2 cut(s) 2191, 3087
Bst2UI CCWGG 4 cut(s) 89, 966, 1023, 1931
Bst4CI ACNGT 4 cut(s) 1351, 3252, 3478, 4203
Bst6I CTCTTC 8 cut(s) 1360, 2247, 2322, 2760, 3528, 3534, 3668, 3942
BstAFI CTTAAG 1 cut(s) 1829
BstAPI GCANNNNNTGC 2 cut(s) 2387, 3139
BstAUI TGTACA 1 cut(s) 3229
BstBAI YACGTR 1 cut(s) 1517
BstBI TTCGAA 1 cut(s) 671
BstC8I GCNNGC 5 cut(s) 1012, 2167, 2171, 3439, 3965
BstENI CCTNNNNNAGG 1 cut(s) 241
BstF5I GGATG 8 cut(s) 37, 44, 748, 1389, 3097, 3347, 3703, 4102
BstFNI CGCG 1 cut(s) 226
BstHHI GCGC 1 cut(s) 2748
BstMAI GTCTC 4 cut(s) 326, 1357, 3265, 3645
BstNI CCWGG 4 cut(s) 89, 966, 1023, 1931
BstNSI RCATGY 8 cut(s) 1014, 1346, 1569, 2093, 2173, 2873, 3269, 3441
BstSCI CCNGG 6 cut(s) 87, 964, 1021, 1929, 3004, 3460
BstSFI CTRYAG 5 cut(s) 780, 1596, 1860, 3816, 4065
BstSLI GKGCMC 2 cut(s) 3109, 4145
BstUI CGCG 1 cut(s) 226
BstV2I GAAGAC 4 cut(s) 99, 324, 1040, 1704
BstX2I RGATCY 9 cut(s) 677, 1573, 1633, 1903, 1911, 1966, 3070, 3279, 3751
BstXI CCANNNNNNTGG 5 cut(s) 1806, 2080, 2729, 3589, 3791
BstYI RGATCY 9 cut(s) 677, 1573, 1633, 1903, 1911, 1966, 3070, 3279, 3751
Bsu15I ATCGAT 2 cut(s) 840, 3762
BsuI GTATCC 1 cut(s) 302
BsuRI GGCC 6 cut(s) 235, 352, 571, 2072, 2337, 2675
BsuTUI ATCGAT 2 cut(s) 840, 3762
BtgZI GCGATG 2 cut(s) 3128, 3905
BtsCI GGATG 8 cut(s) 37, 44, 748, 1389, 3097, 3347, 3703, 4102
BtsIMutI CAGTG 7 cut(s) 467, 845, 1818, 2462, 2913, 3053, 4143
BveI ACCTGC 3 cut(s) 877, 3092, 3124
Cac8I GCNNGC 5 cut(s) 1012, 2167, 2171, 3439, 3965
CaiI CAGNNNCTG 1 cut(s) 3213
CfoI GCGC 1 cut(s) 2748
Cfr13I GGNCC 6 cut(s) 234, 2335, 2673, 2982, 3421, 3862
ClaI ATCGAT 2 cut(s) 840, 3762
CsiI ACCWGGT 1 cut(s) 1021
Csp6I GTAC 9 cut(s) 339, 765, 1937, 2312, 2445, 2692, 3230, 3373, 4204
CviQI GTAC 9 cut(s) 339, 765, 1937, 2312, 2445, 2692, 3230, 3373, 4204
DraI TTTAAA 3 cut(s) 601, 1320, 2508
EaeI YGGCCR 1 cut(s) 2070
Eam1104I CTCTTC 8 cut(s) 1360, 2247, 2322, 2760, 3528, 3534, 3668, 3942
EarI CTCTTC 8 cut(s) 1360, 2247, 2322, 2760, 3528, 3534, 3668, 3942
EciI GGCGGA 1 cut(s) 89
Ecl136II GAGCTC 1 cut(s) 1393
Eco130I CCWWGG 2 cut(s) 2191, 3087
Eco24I GRGCYC 4 cut(s) 453, 1395, 1851, 3227
Eco47I GGWCC 3 cut(s) 2982, 3421, 3862
Eco53kI GAGCTC 1 cut(s) 1393
Eco57I CTGAAG 6 cut(s) 1842, 2382, 3117, 3353, 3509, 3594
Eco88I CYCGRG 1 cut(s) 3188
EcoICRI GAGCTC 1 cut(s) 1393
EcoNI CCTNNNNNAGG 1 cut(s) 241
EcoO109I RGGNCCY 2 cut(s) 2335, 3421
EcoRII CCWGG 4 cut(s) 87, 964, 1021, 1929
EcoT14I CCWWGG 2 cut(s) 2191, 3087
EcoT22I ATGCAT 2 cut(s) 2060, 2091
EcoT38I GRGCYC 4 cut(s) 453, 1395, 1851, 3227
ErhI CCWWGG 2 cut(s) 2191, 3087
FalI AAGNNNNNCTT 2 cut(s) 4152, 4184
FaqI GGGAC 5 cut(s) 1663, 2222, 2968, 3407, 3875
FauI CCCGC 1 cut(s) 3852
FauNDI CATATG 3 cut(s) 2218, 2797, 3669
FbaI TGATCA 2 cut(s) 2266, 2874
FblI GTMKAC 1 cut(s) 1151
FokI GGATG 8 cut(s) 31, 44, 755, 1396, 3104, 3334, 3710, 4089
FriOI GRGCYC 4 cut(s) 453, 1395, 1851, 3227
GlaI GCGC 1 cut(s) 2747
GsaI CCCAGC 2 cut(s) 2929, 3800
GsuI CTGGAG 4 cut(s) 975, 2356, 2420, 4199
HaeIII GGCC 6 cut(s) 235, 352, 571, 2072, 2337, 2675
HapII CCGG 3 cut(s) 686, 3006, 3461
HhaI GCGC 1 cut(s) 2748
Hin6I GCGC 1 cut(s) 2746
HinP1I GCGC 1 cut(s) 2746
HincII GTYRAC 1 cut(s) 1270
HindII GTYRAC 1 cut(s) 1270
HindIII AAGCTT 7 cut(s) 1793, 2438, 2519, 2834, 3738, 3977, 4241
HpaII CCGG 3 cut(s) 686, 3006, 3461
HphI GGTGA 7 cut(s) 380, 485, 1978, 2009, 2053, 3327, 3386
Hpy166II GTNNAC 6 cut(s) 1152, 1186, 1270, 2017, 3107, 4206
Hpy8I GTNNAC 6 cut(s) 1152, 1186, 1270, 2017, 3107, 4206
Hpy99I CGWCG 2 cut(s) 1296, 3159
HpyCH4III ACNGT 4 cut(s) 1351, 3252, 3478, 4203
HpyCH4IV ACGT 9 cut(s) 284, 430, 1073, 1182, 1516, 1939, 2512, 2967, 3721
HpySE526I ACGT 9 cut(s) 284, 430, 1073, 1182, 1516, 1939, 2512, 2967, 3721
HspAI GCGC 1 cut(s) 2746
Kpn2I TCCGGA 1 cut(s) 685
Ksp22I TGATCA 2 cut(s) 2266, 2874
LguI GCTCTTC 1 cut(s) 3528
LmnI GCTCC 8 cut(s) 456, 1398, 1954, 2099, 2206, 2227, 2401, 3838
MabI ACCWGGT 1 cut(s) 1021
MaeII ACGT 9 cut(s) 284, 430, 1073, 1182, 1516, 1939, 2512, 2967, 3721
MbiI CCGCTC 1 cut(s) 1704
MfeI CAATTG 3 cut(s) 2915, 3233, 3432
MflI RGATCY 9 cut(s) 677, 1573, 1633, 1903, 1911, 1966, 3070, 3279, 3751
MhlI GDGCHC 8 cut(s) 453, 1395, 1851, 3083, 3109, 3227, 3886, 4145
MlsI TGGCCA 1 cut(s) 2072
MluNI TGGCCA 1 cut(s) 2072
MlyI GAGTC 8 cut(s) 328, 338, 601, 650, 1289, 2284, 3314, 3612
MmeI TCCRAC 5 cut(s) 267, 642, 978, 2332, 2644
Mox20I TGGCCA 1 cut(s) 2072
Mph1103I ATGCAT 2 cut(s) 2060, 2091
MroI TCCGGA 1 cut(s) 685
MroXI GAANNNNTTC 1 cut(s) 1246
MscI TGGCCA 1 cut(s) 2072
Msp20I TGGCCA 1 cut(s) 2072
MspA1I CMGCKG 3 cut(s) 785, 883, 1478
MspCI CTTAAG 1 cut(s) 1829
MspI CCGG 3 cut(s) 686, 3006, 3461
MspR9I CCNGG 6 cut(s) 89, 966, 1023, 1931, 3006, 3462
MunI CAATTG 3 cut(s) 2915, 3233, 3432
Mva1269I GAATGC 1 cut(s) 2887
MvaI CCWGG 4 cut(s) 89, 966, 1023, 1931
MvnI CGCG 1 cut(s) 226
NciI CCSGG 2 cut(s) 3006, 3462
NdeI CATATG 3 cut(s) 2218, 2797, 3669
NmuCI GTSAC 6 cut(s) 473, 2287, 2458, 3215, 3261, 3392
NsiI ATGCAT 2 cut(s) 2060, 2091
NspI RCATGY 8 cut(s) 1014, 1346, 1569, 2093, 2173, 2873, 3269, 3441
NspV TTCGAA 1 cut(s) 671
OliI CACNNNNGTG 1 cut(s) 1823
PacI TTAATTAA 1 cut(s) 1545
PaeI GCATGC 3 cut(s) 1014, 2173, 3441
PaqCI CACCTGC 2 cut(s) 877, 3092
PciI ACATGT 3 cut(s) 1565, 2869, 3265
PciSI GCTCTTC 1 cut(s) 3528
PctI GAATGC 1 cut(s) 2887
PdmI GAANNNNTTC 1 cut(s) 1246
PflMI CCANNNNNTGG 2 cut(s) 2197, 2351
PleI GAGTC 8 cut(s) 327, 337, 600, 649, 1288, 2284, 3313, 3611
PpsI GAGTC 8 cut(s) 327, 337, 600, 649, 1288, 2284, 3313, 3611
Ppu21I YACGTR 1 cut(s) 1517
PpuMI RGGWCCY 1 cut(s) 3421
PscI ACATGT 3 cut(s) 1565, 2869, 3265
PshBI ATTAAT 4 cut(s) 182, 1197, 4002, 4136
Psp124BI GAGCTC 1 cut(s) 1395
Psp1406I AACGTT 3 cut(s) 430, 2512, 3721
Psp5II RGGWCCY 1 cut(s) 3421
Psp6I CCWGG 4 cut(s) 87, 964, 1021, 1929
PspFI CCCAGC 2 cut(s) 2925, 3796
PspGI CCWGG 4 cut(s) 87, 964, 1021, 1929
PspPI GGNCC 6 cut(s) 234, 2335, 2673, 2982, 3421, 3862
PspPPI RGGWCCY 1 cut(s) 3421
PstI CTGCAG 5 cut(s) 784, 1600, 1864, 3820, 4069
PstNI CAGNNNCTG 1 cut(s) 3213
PsuI RGATCY 9 cut(s) 677, 1573, 1633, 1903, 1911, 1966, 3070, 3279, 3751
PvuII CAGCTG 2 cut(s) 785, 1478
RsaI GTAC 9 cut(s) 340, 766, 1938, 2313, 2446, 2693, 3231, 3374, 4205
RsaNI GTAC 9 cut(s) 339, 765, 1937, 2312, 2445, 2692, 3230, 3373, 4204
SacI GAGCTC 1 cut(s) 1395
SapI GCTCTTC 1 cut(s) 3528
Sau96I GGNCC 6 cut(s) 234, 2335, 2673, 2982, 3421, 3862
ScaI AGTACT 2 cut(s) 340, 3374
SchI GAGTC 8 cut(s) 328, 338, 601, 650, 1289, 2284, 3314, 3612
ScrFI CCNGG 6 cut(s) 89, 966, 1023, 1931, 3006, 3462
SduI GDGCHC 8 cut(s) 453, 1395, 1851, 3083, 3109, 3227, 3886, 4145
SexAI ACCWGGT 1 cut(s) 1021
SfcI CTRYAG 5 cut(s) 780, 1596, 1860, 3816, 4065
SfuI TTCGAA 1 cut(s) 671
SinI GGWCC 3 cut(s) 2982, 3421, 3862
SmlI CTYRAG 5 cut(s) 1590, 1750, 1829, 2739, 3283
SmoI CTYRAG 5 cut(s) 1590, 1750, 1829, 2739, 3283
SpeI ACTAGT 1 cut(s) 3514
SphI GCATGC 3 cut(s) 1014, 2173, 3441
SsiI CCGC 8 cut(s) 74, 224, 280, 740, 883, 1702, 3691, 3859
SstI GAGCTC 1 cut(s) 1395
StyD4I CCNGG 6 cut(s) 87, 964, 1021, 1929, 3004, 3460
StyI CCWWGG 2 cut(s) 2191, 3087
TaaI ACNGT 4 cut(s) 1351, 3252, 3478, 4203
TaiI ACGT 9 cut(s) 287, 433, 1076, 1185, 1519, 1942, 2515, 2970, 3724
TaqI TCGA 9 cut(s) 335, 671, 840, 2605, 2758, 2891, 3157, 3762, 3945
TatI WGTACW 6 cut(s) 338, 2444, 2691, 3229, 3372, 4203
TauI GCSGC 2 cut(s) 886, 3694
TscAI CASTG 7 cut(s) 467, 852, 1825, 2469, 2913, 3060, 4150
TseFI GTSAC 6 cut(s) 473, 2287, 2458, 3215, 3261, 3392
Tsp45I GTSAC 6 cut(s) 473, 2287, 2458, 3215, 3261, 3392
TspGWI ACGGA 2 cut(s) 522, 3143
TspRI CASTG 7 cut(s) 467, 852, 1825, 2469, 2913, 3060, 4150
Van91I CCANNNNNTGG 2 cut(s) 2197, 2351
Vha464I CTTAAG 1 cut(s) 1829
VneI GTGCAC 1 cut(s) 3105
VpaK11BI GGWCC 3 cut(s) 2982, 3421, 3862
VspI ATTAAT 4 cut(s) 182, 1197, 4002, 4136
XagI CCTNNNNNAGG 1 cut(s) 241
XapI RAATTY 3 cut(s) 394, 420, 4115
XbaI TCTAGA 3 cut(s) 806, 1888, 2051
XceI RCATGY 8 cut(s) 1014, 1346, 1569, 2093, 2173, 2873, 3269, 3441
XcmI CCANNNNNNNNNTGG 2 cut(s) 1165, 3041
XmiI GTMKAC 1 cut(s) 1151
XmnI GAANNNNTTC 1 cut(s) 1246
ZrmI AGTACT 2 cut(s) 340, 3374
Zsp2I ATGCAT 2 cut(s) 2060, 2091
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.