MD03G1007100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0013150
Physical Location & Seq
Forward (+)
572944 .. 575174
2231 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1007100.v1.1.491

Sequence Viewer

Length: 1242 bp
ATGGAGTCGATTCCTCTGAGGCATTCAATCTCTGCAACCAATCAGAGCTTTCTATTAAAAAGACAAAGAAACCCAGAAAATCAATTTCAGAGCAGCAGCCGAAGGTTTCACCTTCACACCCCAACTCGGCGTTTTCGAATCCATTCCAAACTCTCTGGTTTTCGAGATTCCAAGGGCTATGCAAGGCCTTCGCGTCTCTTGCCAGCTACAGACGTGAAAATCTGCACAGATAACTCAGTGGAAAAGCTTTTCTCGTCTTTAAGGGATGACGGATCTCAGTCTTTATACAAGGTTAAGTTAGGTACTATCAATGTTTATGGCTCAAGTCTCTCTGACTTGAATGCTGGGATCCTCCTTTGCGTGATAGACGAAAATGGTGACTCCATATTACAGAGGATACCGTCGAGTATCATAACAAATCATGCTACAGAGTCGGAGGATGGCGTGCTTCACTTTCAAAGAGGTTCTGTTGATGAGTTCACCTTTGAGGGACCTAAGCTTGGAAAAGTTGGAGCTGTTTGGATCAGTCTTGAATCAGGTCAATGGCGGTTAGGAAGTGTGAGCTTGACCGTTATCTGTGGGAATCAAAGTTCGTTGGAAGAAAAAGACGAAGAAAAACTTCAGTATACTGGTTTCAGTTACAACTTTGCTGTTGAGGATATCTTGCTGGGTGAGGGAAGTGACAGTTCCATGGTGCAACTTAGACCTTGCCTTGTGACCGAGCTATCTGGGGTTGACCCCGTCACCATCTTCACCAAAAGCCTCCCTGAATCAACTCTACCTGTGAGTGGGATATCAAACGAGGAAACCATGAGAGAATACGAGGATTTGAAACTATCTTTGCTACTTTATGATGCCATGCTGATCTTTGTTGGTACATCAGTCGCATCCTTCTCAGCTGGGGAAAACATAAGCTTTGCATTTTTAACAGGTGGGATGGGCGGCTTCTTCTATCTCTTGCTACTGCAAAGGTCGGTTGATGGATTACCCGCAGCAGAATTGACTTCCACGAACACAGGACAGACCAACCAAACCTTCGGAGGATCTAAGGTTCCAATATTTGTCTTTGCATTGGCTATTGGTTTTACTTTACTCACAGTGAAGTATGGCTCCGGGGATGTTCCAATCGTATTCACTCCGAAACAACTTATAGCCGGGATGCTTGGATTTCTTGCATGTAAAGTTTCTGTGGTCCTGGCAGCAGTTAAGCCCTTGCCAATAAGTCTAAAGATAAACGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

414

Amino Acids

45.02

Weight (kDa)

5.8

Isoelectric Point (pI)

39.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7755 PF24938 94 - 240 5.4e-64 Domain of unknown function (DUF7755)
CGL160_C PF24763 267 - 402 6.9e-06 CGL160, C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013150)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55280 AT1G55280
fragaria_vesca FvH4_3g45230
malus_domestica MD03G1007100.v1.1
prunus_persica Prupe.6G006700_v2.0.a1
pyrus_communis pycom03g00720
rosa_chinensis RchiOBHm_Chr5g0082841
rosa_laevigata RLG00000036994
rosa_multiflora Rmu_sc0001381.1_g000013 Rmu_sc0007172.1_g000002
rosa_roxburghii Rroxscaffold_1G00001590
rosa_rugosa Rorug05G0480300
rosa_samantha Rh5AG530600 Rh5AG530700 Rh5BG557600 Rh5CG580600 Rh5DG565900
rosa_wichuraiana Rw5G049800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 626
AccII CGCG 1 cut(s) 193
AciI CCGC 3 cut(s) 547, 942, 990
AclWI GGATC 5 cut(s) 280, 343, 356, 530, 1051
AcuI CTGAAG 1 cut(s) 605
AfaI GTAC 2 cut(s) 304, 877
AfiI CCNNNNNNNGG 3 cut(s) 126, 500, 788
AgsI TTSAA 5 cut(s) 27, 340, 458, 533, 832
AjiI CACGTC 1 cut(s) 214
AjnI CCWGG 1 cut(s) 1194
AjuI GAANNNNNNNTTGG 2 cut(s) 1019, 1051
AloI GAACNNNNNNTCC 4 cut(s) 574, 606, 1035, 1067
AluBI AGCT 9 cut(s) 48, 206, 247, 499, 515, 564, 724, 899, 915
AluI AGCT 9 cut(s) 48, 206, 247, 499, 515, 564, 724, 899, 915
Alw26I GTCTC 2 cut(s) 200, 332
AlwI GGATC 5 cut(s) 280, 343, 356, 530, 1051
AoxI GGCC 1 cut(s) 185
ApeKI GCWGC 4 cut(s) 93, 96, 992, 1199
Asp700I GAANNNNTTC 2 cut(s) 142, 618
AspS9I GGNCC 2 cut(s) 491, 1192
AsuC2I CCSGG 2 cut(s) 1114, 1156
AsuHPI GGTGA 6 cut(s) 101, 389, 472, 683, 736, 745
AsuII TTCGAA 1 cut(s) 136
AvaII GGWCC 2 cut(s) 491, 1192
BaeI ACNNNNGTAYC 2 cut(s) 867, 900
BamHI GGATCC 1 cut(s) 348
BbvI GCAGC 4 cut(s) 105, 108, 1004, 1211
BccI CCATC 4 cut(s) 434, 755, 931, 974
BcgI CGANNNNNNTGC 2 cut(s) 171, 205
BciT130I CCWGG 1 cut(s) 1196
BciVI GTATCC 1 cut(s) 390
BcnI CCSGG 2 cut(s) 1114, 1156
BcoDI GTCTC 2 cut(s) 200, 332
BfmI CTRYAG 2 cut(s) 207, 426
BfuI GTATCC 1 cut(s) 390
BisI GCNGC 5 cut(s) 94, 97, 943, 993, 1200
BlsI GCNGC 5 cut(s) 95, 98, 944, 994, 1201
Bme1390I CCNGG 3 cut(s) 1114, 1156, 1196
Bme18I GGWCC 2 cut(s) 491, 1192
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 2 cut(s) 491, 1192
BmiI GGNNCC 4 cut(s) 350, 492, 1053, 1111
BmrFI CCNGG 3 cut(s) 1114, 1156, 1196
BmsI GCATC 3 cut(s) 844, 896, 1149
Bpu10I CCTNAGC 1 cut(s) 495
Bpu14I TTCGAA 1 cut(s) 136
BpuEI CTTGAG 1 cut(s) 307
BpuMI CCSGG 2 cut(s) 1114, 1156
BsaJI CCNNGG 3 cut(s) 171, 690, 1113
Bsc4I CCNNNNNNNGG 3 cut(s) 126, 500, 788
Bse1I ACTGG 1 cut(s) 634
BseBI CCWGG 1 cut(s) 1196
BseDI CCNNGG 3 cut(s) 171, 690, 1113
BseGI GGATG 6 cut(s) 271, 445, 887, 942, 1123, 1164
BseLI CCNNNNNNNGG 3 cut(s) 126, 500, 788
BseMII CTCAG 4 cut(s) 8, 249, 290, 909
BseNI ACTGG 1 cut(s) 634
BseXI GCAGC 4 cut(s) 105, 108, 1004, 1211
BseYI CCCAGC 3 cut(s) 344, 667, 899
BsgI GTGCAG 1 cut(s) 208
Bsh1236I CGCG 1 cut(s) 193
BshFI GGCC 1 cut(s) 187
BsiSI CCGG 2 cut(s) 1113, 1155
BslFI GGGAC 1 cut(s) 504
BslI CCNNNNNNNGG 3 cut(s) 126, 500, 788
BsmAI GTCTC 2 cut(s) 200, 332
BsmBI CGTCTC 1 cut(s) 200
BsmFI GGGAC 1 cut(s) 504
BsmI GAATGC 2 cut(s) 22, 346
BsnI GGCC 1 cut(s) 187
Bsp119I TTCGAA 1 cut(s) 136
Bsp143I GATC 5 cut(s) 272, 348, 522, 864, 1043
Bsp19I CCATGG 1 cut(s) 690
BspACI CCGC 3 cut(s) 547, 942, 990
BspANI GGCC 1 cut(s) 187
BspCNI CTCAG 4 cut(s) 9, 248, 289, 908
BspFNI CGCG 1 cut(s) 193
BspLI GGNNCC 4 cut(s) 350, 492, 1053, 1111
BspPI GGATC 5 cut(s) 280, 343, 356, 530, 1051
BspT104I TTCGAA 1 cut(s) 136
BsrI ACTGG 1 cut(s) 634
BssECI CCNNGG 3 cut(s) 171, 690, 1113
BssMI GATC 5 cut(s) 272, 348, 522, 864, 1043
BssNAI GTATAC 1 cut(s) 627
BssT1I CCWWGG 2 cut(s) 171, 690
Bst1107I GTATAC 1 cut(s) 627
Bst2UI CCWGG 1 cut(s) 1196
Bst4CI ACNGT 4 cut(s) 402, 571, 686, 1099
BstBI TTCGAA 1 cut(s) 136
BstC8I GCNNGC 2 cut(s) 204, 446
BstDEI CTNAG 7 cut(s) 17, 235, 276, 495, 701, 895, 1047
BstDSI CCRYGG 1 cut(s) 690
BstF5I GGATG 6 cut(s) 271, 445, 887, 942, 1123, 1164
BstFNI CGCG 1 cut(s) 193
BstKTI GATC 5 cut(s) 275, 351, 525, 867, 1046
BstMAI GTCTC 2 cut(s) 200, 332
BstMBI GATC 5 cut(s) 272, 348, 522, 864, 1043
BstMWI GCNNNNNNNGC 1 cut(s) 199
BstNI CCWGG 1 cut(s) 1196
BstNSI RCATGY 1 cut(s) 1179
BstSCI CCNGG 3 cut(s) 1112, 1154, 1194
BstSFI CTRYAG 2 cut(s) 207, 426
BstUI CGCG 1 cut(s) 193
BstV1I GCAGC 4 cut(s) 105, 108, 1004, 1211
BstX2I RGATCY 3 cut(s) 272, 348, 1043
BstYI RGATCY 3 cut(s) 272, 348, 1043
BstZ17I GTATAC 1 cut(s) 627
BsuI GTATCC 1 cut(s) 390
BsuRI GGCC 1 cut(s) 187
BtgI CCRYGG 1 cut(s) 690
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 6 cut(s) 271, 445, 887, 942, 1123, 1164
BtsIMutI CAGTG 2 cut(s) 243, 1104
Cac8I GCNNGC 2 cut(s) 204, 446
Cfr13I GGNCC 2 cut(s) 491, 1192
CseI GACGC 1 cut(s) 182
Csp6I GTAC 2 cut(s) 303, 876
CviAII CATG 5 cut(s) 422, 691, 811, 859, 1176
CviQI GTAC 2 cut(s) 303, 876
DdeI CTNAG 7 cut(s) 17, 235, 276, 495, 701, 895, 1047
DpnI GATC 5 cut(s) 274, 350, 524, 866, 1045
DpnII GATC 5 cut(s) 272, 348, 522, 864, 1043
Eco130I CCWWGG 2 cut(s) 171, 690
Eco147I AGGCCT 1 cut(s) 187
Eco32I GATATC 2 cut(s) 661, 795
Eco47I GGWCC 2 cut(s) 491, 1192
Eco57I CTGAAG 1 cut(s) 605
EcoO109I RGGNCCY 1 cut(s) 491
EcoRII CCWGG 1 cut(s) 1194
EcoRV GATATC 2 cut(s) 661, 795
EcoT14I CCWWGG 2 cut(s) 171, 690
ErhI CCWWGG 2 cut(s) 171, 690
Esp3I CGTCTC 1 cut(s) 200
FaeI CATG 5 cut(s) 425, 694, 814, 862, 1179
FalI AAGNNNNNCTT 2 cut(s) 603, 635
FaqI GGGAC 1 cut(s) 504
FatI CATG 5 cut(s) 421, 690, 810, 858, 1175
FauI CCCGC 1 cut(s) 997
FblI GTMKAC 1 cut(s) 626
Fnu4HI GCNGC 5 cut(s) 94, 97, 943, 993, 1200
FokI GGATG 6 cut(s) 278, 452, 874, 949, 1130, 1171
Fsp4HI GCNGC 5 cut(s) 94, 97, 943, 993, 1200
GluI GCNGC 5 cut(s) 94, 97, 943, 993, 1200
GsaI CCCAGC 3 cut(s) 348, 671, 903
HaeIII GGCC 1 cut(s) 187
HapII CCGG 2 cut(s) 1113, 1155
HgaI GACGC 1 cut(s) 182
Hin1II CATG 5 cut(s) 425, 694, 814, 862, 1179
HincII GTYRAC 1 cut(s) 736
HindII GTYRAC 1 cut(s) 736
HindIII AAGCTT 3 cut(s) 245, 497, 913
HinfI GANTC 9 cut(s) 5, 10, 138, 167, 380, 431, 533, 583, 770
HpaII CCGG 2 cut(s) 1113, 1155
HphI GGTGA 6 cut(s) 101, 389, 472, 683, 736, 745
Hpy166II GTNNAC 3 cut(s) 480, 627, 736
Hpy188I TCNGA 7 cut(s) 18, 45, 90, 334, 436, 1040, 1140
Hpy188III TCNNGA 2 cut(s) 164, 530
Hpy8I GTNNAC 3 cut(s) 480, 627, 736
Hpy99I CGWCG 1 cut(s) 406
HpyAV CCTTC 5 cut(s) 96, 122, 198, 901, 1045
HpyCH4III ACNGT 4 cut(s) 402, 571, 686, 1099
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 8 cut(s) 35, 182, 225, 697, 920, 967, 1070, 1175
HpyF10VI GCNNNNNNNGC 1 cut(s) 199
HpyF3I CTNAG 7 cut(s) 17, 235, 276, 495, 701, 895, 1047
HpySE526I ACGT 1 cut(s) 213
Hsp92II CATG 5 cut(s) 425, 694, 814, 862, 1179
Kzo9I GATC 5 cut(s) 272, 348, 522, 864, 1043
LmnI GCTCC 2 cut(s) 512, 1115
Lsp1109I GCAGC 4 cut(s) 105, 108, 1004, 1211
LweI GCATC 3 cut(s) 844, 896, 1149
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 5 cut(s) 377, 638, 680, 715, 742
MalI GATC 5 cut(s) 274, 350, 524, 866, 1045
MboI GATC 5 cut(s) 272, 348, 522, 864, 1043
MboII GAAGA 4 cut(s) 611, 623, 742, 940
MflI RGATCY 3 cut(s) 272, 348, 1043
MluCI AATT 2 cut(s) 83, 998
MlyI GAGTC 3 cut(s) 14, 374, 440
MmeI TCCRAC 3 cut(s) 414, 490, 576
MroXI GAANNNNTTC 2 cut(s) 142, 618
MseI TTAA 5 cut(s) 56, 260, 294, 926, 1206
MspA1I CMGCKG 1 cut(s) 899
MspI CCGG 2 cut(s) 1113, 1155
MspR9I CCNGG 3 cut(s) 1114, 1156, 1196
Mva1269I GAATGC 2 cut(s) 22, 346
MvaI CCWGG 1 cut(s) 1196
MvnI CGCG 1 cut(s) 193
MwoI GCNNNNNNNGC 1 cut(s) 199
NciI CCSGG 2 cut(s) 1114, 1156
NcoI CCATGG 1 cut(s) 690
NdeII GATC 5 cut(s) 272, 348, 522, 864, 1043
NlaIII CATG 5 cut(s) 425, 694, 814, 862, 1179
NlaIV GGNNCC 4 cut(s) 350, 492, 1053, 1111
NmeAIII GCCGAG 1 cut(s) 106
NmuCI GTSAC 4 cut(s) 377, 680, 715, 742
NspI RCATGY 1 cut(s) 1179
NspV TTCGAA 1 cut(s) 136
PceI AGGCCT 1 cut(s) 187
PcsI WCGNNNNNNNCGW 1 cut(s) 133
PctI GAATGC 2 cut(s) 22, 346
PdmI GAANNNNTTC 2 cut(s) 142, 618
PfeI GAWTC 6 cut(s) 10, 138, 167, 533, 583, 770
PflFI GACNNNGTC 1 cut(s) 740
PkrI GCNGC 5 cut(s) 95, 98, 944, 994, 1201
PleI GAGTC 3 cut(s) 13, 374, 439
PpsI GAGTC 3 cut(s) 13, 374, 439
PpuMI RGGWCCY 1 cut(s) 491
Psp5II RGGWCCY 1 cut(s) 491
Psp6I CCWGG 1 cut(s) 1194
PspFI CCCAGC 3 cut(s) 344, 667, 899
PspGI CCWGG 1 cut(s) 1194
PspN4I GGNNCC 4 cut(s) 350, 492, 1053, 1111
PspPI GGNCC 2 cut(s) 491, 1192
PspPPI RGGWCCY 1 cut(s) 491
PsuI RGATCY 3 cut(s) 272, 348, 1043
PsyI GACNNNGTC 1 cut(s) 740
PvuII CAGCTG 1 cut(s) 899
RsaI GTAC 2 cut(s) 304, 877
RsaNI GTAC 2 cut(s) 303, 876
SaqAI TTAA 5 cut(s) 56, 260, 294, 926, 1206
SatI GCNGC 5 cut(s) 94, 97, 943, 993, 1200
Sau3AI GATC 5 cut(s) 272, 348, 522, 864, 1043
Sau96I GGNCC 2 cut(s) 491, 1192
SchI GAGTC 3 cut(s) 14, 374, 440
ScrFI CCNGG 3 cut(s) 1114, 1156, 1196
SfaNI GCATC 3 cut(s) 844, 896, 1149
SfcI CTRYAG 2 cut(s) 207, 426
SfuI TTCGAA 1 cut(s) 136
SinI GGWCC 2 cut(s) 491, 1192
SmlI CTYRAG 1 cut(s) 322
SmoI CTYRAG 1 cut(s) 322
Sse9I AATT 2 cut(s) 83, 998
SseBI AGGCCT 1 cut(s) 187
SsiI CCGC 3 cut(s) 547, 942, 990
SspI AATATT 1 cut(s) 1059
StuI AGGCCT 1 cut(s) 187
StyD4I CCNGG 3 cut(s) 1112, 1154, 1194
StyI CCWWGG 2 cut(s) 171, 690
TaaI ACNGT 4 cut(s) 402, 571, 686, 1099
TaiI ACGT 1 cut(s) 216
TaqI TCGA 4 cut(s) 8, 136, 163, 404
TaqII GACCGA 1 cut(s) 734
TasI AATT 2 cut(s) 83, 998
TauI GCSGC 1 cut(s) 945
TfiI GAWTC 6 cut(s) 10, 138, 167, 533, 583, 770
Tru1I TTAA 5 cut(s) 56, 260, 294, 926, 1206
Tru9I TTAA 5 cut(s) 56, 260, 294, 926, 1206
TscAI CASTG 2 cut(s) 243, 1104
TseFI GTSAC 4 cut(s) 377, 680, 715, 742
TseI GCWGC 4 cut(s) 93, 96, 992, 1199
Tsp45I GTSAC 4 cut(s) 377, 680, 715, 742
TspGWI ACGGA 1 cut(s) 285
TspRI CASTG 2 cut(s) 243, 1104
Tth111I GACNNNGTC 1 cut(s) 740
VpaK11BI GGWCC 2 cut(s) 491, 1192
XceI RCATGY 1 cut(s) 1179
XmiI GTMKAC 1 cut(s) 626
XmnI GAANNNNTTC 2 cut(s) 142, 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.