Rw5G049800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0013150
Physical Location & Seq
Forward (+)
86346948 .. 86349131
2184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G049800.1

Sequence Viewer

Length: 1344 bp
ATGACAGGCCTGTTCACTCTTATATCGACTTTGATTTCCATTTTGATGATCGAGAATCTGTTACAACTCTCTTTCATTCGTTCCGTGTCTCAGCTTTTCTCCGAAACTGCAACTATGGAGTCCATTTCCTTAAGGCATGCAATCTCTGCAACACATCACACCTATCTCAGGAGAACAAGAGTAAACCCAGAAAAGCCATTTCAGACAAAAAGGTTTCACCTTCACAACCCAACCGGCCGTTTTCAAATCCATTCCAAGCTCTCCGATTTCCAAGACTATGCAAAGCCTTCACGCCTATTGCAAGCCACAGAAGTGAATGTCTGCACAGATACATCAGTGGAAAAAAGCTTCTTGTCATTAAGGGATTATGAATGTCAGGCTTTATATAAGGTCAAGCTAGGTACTAGTAATGCTTATGGTTCAAGTCTCTCCGACCTTACTGCTGGAATCCTCCTGTGCATGATAGACGAAAATGGTAACTCCATACTACAGAGGATACCCTCGAATTTGAGACCGGATGAATCTAAAGAAGTAGAGGATGAGATAATTCAGTTTCAAAGAGGTTCTGTTGATGAGTTCACCTTTAAGGGACCTAAACTTGGAAAAGTTGAAGCTCTTTGGATCAGTCTTGAATCAGGCCAATGGAGGTTAGGAAGTGTGAACTTGACTGTTATATATGGGAATCATATTTCTTTGGAGGAAACAGATGGAGAGGAACCTCAGTATATTGGTTTTGATTACAACTTCCAAGCTGAGGACATCTTGCTCGGAGAGGGAAGTGACAGCTCCATGGTGGAACTTAGACCTTGCCTTGTCACCAAGCTATCTGGGGTTGACCCCTTCACCATGTTGACTAAAAGCCTCCCTGAATCAACTCTATCCGAGAGCCGTGGGATATCAAATGAGGAAACCATGAGAGAATACGCGGATTTGAAGTTCTCTTTGCTACTTTACGATGCCATACTGGTCTTGGTTGGTACATCAGTTGCATCCTTTTCAGTTGGGGAAAATGCCGGTTCTGCATTTTTAACAGGTGGGATTGGTGGCTTCTTGTATCTTCTTCTACTGCAAAGGTCAGTTGATGGATTACCTATTCCAGAATCAATTTCCGTGGACAGCAAAAGCACCAATCAAATGTTTGGAGGAATGAAGAGGCCAATCTTTGGCCTTGCGTTGGCTCTTGGTTTTGCTTTATTTACAGTGAAGTATAGCTCTGGGAATGTCCCAATGGTTTTCACAGCAAAAGAAGTCTTGGCTGGGATGGTGGGATTTCTTGCATGTAAAGTTGCTGTGGTTTTGGCAGCAATTAAGCCCATGACAATAGACCTAAAGATAAAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

447

Amino Acids

49.25

Weight (kDa)

5.36

Isoelectric Point (pI)

37.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7755 PF24938 127 - 273 2.6e-63 Domain of unknown function (DUF7755)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013150)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55280 AT1G55280
fragaria_vesca FvH4_3g45230
malus_domestica MD03G1007100.v1.1
prunus_persica Prupe.6G006700_v2.0.a1
pyrus_communis pycom03g00720
rosa_chinensis RchiOBHm_Chr5g0082841
rosa_laevigata RLG00000036994
rosa_multiflora Rmu_sc0001381.1_g000013 Rmu_sc0007172.1_g000002
rosa_roxburghii Rroxscaffold_1G00001590
rosa_rugosa Rorug05G0480300
rosa_samantha Rh5AG530600 Rh5AG530700 Rh5BG557600 Rh5CG580600 Rh5DG565900
rosa_wichuraiana Rw5G049800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1163
AccII CGCG 1 cut(s) 926
AciI CCGC 1 cut(s) 926
AclWI GGATC 1 cut(s) 629
AcoI YGGCCR 1 cut(s) 235
AcsI RAATTY 1 cut(s) 505
AfaI GTAC 2 cut(s) 403, 979
AfiI CCNNNNNNNGG 5 cut(s) 168, 599, 754, 1163, 1174
AflII CTTAAG 1 cut(s) 130
AgsI TTSAA 6 cut(s) 245, 423, 557, 611, 632, 934
AhlI ACTAGT 1 cut(s) 404
AleI CACNNNNGTG 1 cut(s) 311
AloI GAACNNNNNNTCC 2 cut(s) 920, 952
AluBI AGCT 9 cut(s) 94, 259, 348, 397, 614, 752, 786, 823, 1212
AluI AGCT 9 cut(s) 94, 259, 348, 397, 614, 752, 786, 823, 1212
Alw26I GTCTC 3 cut(s) 93, 431, 505
AlwI GGATC 1 cut(s) 629
AoxI GGCC 5 cut(s) 7, 235, 637, 1154, 1165
ApeKI GCWGC 1 cut(s) 1301
ApoI RAATTY 1 cut(s) 505
AspS9I GGNCC 1 cut(s) 590
AsuHPI GGTGA 4 cut(s) 209, 571, 808, 835
AvaII GGWCC 1 cut(s) 590
BaeI ACNNNNGTAYC 4 cut(s) 321, 354, 969, 1002
BbvCI CCTCAGC 1 cut(s) 753
BbvI GCAGC 1 cut(s) 1313
BccI CCATC 3 cut(s) 701, 1076, 1255
BceAI ACGGC 2 cut(s) 222, 873
BcgI CGANNNNNNTGC 2 cut(s) 422, 456
BciVI GTATCC 1 cut(s) 489
BcoDI GTCTC 3 cut(s) 93, 431, 505
BcuI ACTAGT 1 cut(s) 404
BfaI CTAG 2 cut(s) 398, 405
BfmI CTRYAG 1 cut(s) 488
BfrI CTTAAG 1 cut(s) 130
BfuI GTATCC 1 cut(s) 489
BisI GCNGC 1 cut(s) 1302
BlsI GCNGC 1 cut(s) 1303
Bme18I GGWCC 1 cut(s) 590
BmgT120I GGNCC 1 cut(s) 590
BmiI GGNNCC 2 cut(s) 591, 717
BmsI GCATC 2 cut(s) 946, 998
Bpu10I CCTNAGC 1 cut(s) 753
BsaI GGTCTC 1 cut(s) 505
BsaJI CCNNGG 3 cut(s) 789, 889, 1110
BsaWI WCCGGW 1 cut(s) 514
Bsc4I CCNNNNNNNGG 5 cut(s) 168, 599, 754, 1163, 1174
Bse118I RCCGGY 2 cut(s) 233, 1013
Bse1I ACTGG 1 cut(s) 969
BseDI CCNNGG 3 cut(s) 789, 889, 1110
BseGI GGATG 4 cut(s) 523, 544, 989, 1266
BseLI CCNNNNNNNGG 5 cut(s) 168, 599, 754, 1163, 1174
BseMII CTCAG 4 cut(s) 104, 181, 734, 744
BseNI ACTGG 1 cut(s) 969
BseX3I CGGCCG 1 cut(s) 235
BseXI GCAGC 1 cut(s) 1313
BseYI CCCAGC 1 cut(s) 1256
BsgI GTGCAG 1 cut(s) 307
Bsh1236I CGCG 1 cut(s) 926
Bsh1285I CGRYCG 1 cut(s) 238
BshFI GGCC 5 cut(s) 9, 237, 639, 1156, 1167
BsiEI CGRYCG 1 cut(s) 238
BsiSI CCGG 3 cut(s) 234, 515, 1014
BslFI GGGAC 2 cut(s) 603, 1208
BslI CCNNNNNNNGG 5 cut(s) 168, 599, 754, 1163, 1174
BsmAI GTCTC 3 cut(s) 93, 431, 505
BsmFI GGGAC 2 cut(s) 603, 1208
BsnI GGCC 5 cut(s) 9, 237, 639, 1156, 1167
Bso31I GGTCTC 1 cut(s) 505
Bsp143I GATC 2 cut(s) 48, 621
Bsp19I CCATGG 1 cut(s) 789
BspACI CCGC 1 cut(s) 926
BspANI GGCC 5 cut(s) 9, 237, 639, 1156, 1167
BspCNI CTCAG 4 cut(s) 103, 180, 733, 745
BspFNI CGCG 1 cut(s) 926
BspLI GGNNCC 2 cut(s) 591, 717
BspPI GGATC 1 cut(s) 629
BspTI CTTAAG 1 cut(s) 130
BspTNI GGTCTC 1 cut(s) 505
BsrFI RCCGGY 2 cut(s) 233, 1013
BsrI ACTGG 1 cut(s) 969
BssAI RCCGGY 2 cut(s) 233, 1013
BssECI CCNNGG 3 cut(s) 789, 889, 1110
BssMI GATC 2 cut(s) 48, 621
BssT1I CCWWGG 1 cut(s) 789
Bst4CI ACNGT 2 cut(s) 670, 1201
Bst6I CTCTTC 1 cut(s) 1145
BstAFI CTTAAG 1 cut(s) 130
BstAPI GCANNNNNTGC 1 cut(s) 146
BstC8I GCNNGC 2 cut(s) 138, 303
BstDEI CTNAG 5 cut(s) 90, 167, 720, 753, 800
BstDSI CCRYGG 3 cut(s) 789, 889, 1110
BstENI CCTNNNNNAGG 1 cut(s) 166
BstF5I GGATG 4 cut(s) 523, 544, 989, 1266
BstFNI CGCG 1 cut(s) 926
BstKTI GATC 2 cut(s) 51, 624
BstMAI GTCTC 3 cut(s) 93, 431, 505
BstMBI GATC 2 cut(s) 48, 621
BstMCI CGRYCG 1 cut(s) 238
BstMWI GCNNNNNNNGC 2 cut(s) 146, 1019
BstNSI RCATGY 2 cut(s) 140, 1281
BstSFI CTRYAG 1 cut(s) 488
BstUI CGCG 1 cut(s) 926
BstV1I GCAGC 1 cut(s) 1313
BstZI CGGCCG 1 cut(s) 235
BsuI GTATCC 1 cut(s) 489
BsuRI GGCC 5 cut(s) 9, 237, 639, 1156, 1167
BtgI CCRYGG 3 cut(s) 789, 889, 1110
BtsCI GGATG 4 cut(s) 523, 544, 989, 1266
BtsIMutI CAGTG 2 cut(s) 342, 1206
Cac8I GCNNGC 2 cut(s) 138, 303
Cfr10I RCCGGY 2 cut(s) 233, 1013
Cfr13I GGNCC 1 cut(s) 590
Csp6I GTAC 2 cut(s) 402, 978
CspCI CAANNNNNGTGG 2 cut(s) 1092, 1127
CviAII CATG 7 cut(s) 137, 460, 790, 847, 913, 1278, 1315
CviQI GTAC 2 cut(s) 402, 978
DdeI CTNAG 5 cut(s) 90, 167, 720, 753, 800
DpnI GATC 2 cut(s) 50, 623
DpnII GATC 2 cut(s) 48, 621
EaeI YGGCCR 1 cut(s) 235
EagI CGGCCG 1 cut(s) 235
Eam1104I CTCTTC 1 cut(s) 1145
EarI CTCTTC 1 cut(s) 1145
EclXI CGGCCG 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 789
Eco147I AGGCCT 1 cut(s) 9
Eco31I GGTCTC 1 cut(s) 505
Eco32I GATATC 1 cut(s) 897
Eco47I GGWCC 1 cut(s) 590
Eco52I CGGCCG 1 cut(s) 235
EcoNI CCTNNNNNAGG 1 cut(s) 166
EcoO109I RGGNCCY 1 cut(s) 590
EcoRV GATATC 1 cut(s) 897
EcoT14I CCWWGG 1 cut(s) 789
ErhI CCWWGG 1 cut(s) 789
FaeI CATG 7 cut(s) 140, 463, 793, 850, 916, 1281, 1318
FaqI GGGAC 2 cut(s) 603, 1208
FatI CATG 7 cut(s) 136, 459, 789, 846, 912, 1277, 1314
Fnu4HI GCNGC 1 cut(s) 1302
FokI GGATG 4 cut(s) 530, 551, 976, 1273
Fsp4HI GCNGC 1 cut(s) 1302
FspBI CTAG 2 cut(s) 398, 405
GluI GCNGC 1 cut(s) 1302
GsaI CCCAGC 1 cut(s) 1260
HaeIII GGCC 5 cut(s) 9, 237, 639, 1156, 1167
HapII CCGG 3 cut(s) 234, 515, 1014
Hin1II CATG 7 cut(s) 140, 463, 793, 850, 916, 1281, 1318
HincII GTYRAC 2 cut(s) 835, 852
HindII GTYRAC 2 cut(s) 835, 852
HindIII AAGCTT 1 cut(s) 346
HinfI GANTC 8 cut(s) 55, 119, 447, 521, 632, 682, 869, 1100
HpaII CCGG 3 cut(s) 234, 515, 1014
HphI GGTGA 4 cut(s) 209, 571, 808, 835
Hpy166II GTNNAC 7 cut(s) 15, 184, 579, 661, 835, 852, 1114
Hpy188I TCNGA 6 cut(s) 103, 204, 265, 433, 770, 883
Hpy188III TCNNGA 4 cut(s) 52, 169, 629, 1097
Hpy8I GTNNAC 7 cut(s) 15, 184, 579, 661, 835, 852, 1114
HpyAV CCTTC 3 cut(s) 230, 297, 850
HpyCH4III ACNGT 2 cut(s) 670, 1201
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 1019
HpyF3I CTNAG 5 cut(s) 90, 167, 720, 753, 800
Hsp92II CATG 7 cut(s) 140, 463, 793, 850, 916, 1281, 1318
Kzo9I GATC 2 cut(s) 48, 621
LmnI GCTCC 1 cut(s) 791
Lsp1109I GCAGC 1 cut(s) 1313
LweI GCATC 2 cut(s) 946, 998
MaeI CTAG 2 cut(s) 398, 405
MaeIII GTNAC 4 cut(s) 60, 476, 779, 814
MalI GATC 2 cut(s) 50, 623
MboI GATC 2 cut(s) 48, 621
MboII GAAGA 3 cut(s) 1049, 1052, 1162
MluCI AATT 4 cut(s) 505, 546, 1104, 1305
MlyI GAGTC 1 cut(s) 128
MmeI TCCRAC 1 cut(s) 456
MseI TTAA 5 cut(s) 131, 359, 585, 1028, 1308
MslI CAYNNNNRTG 2 cut(s) 44, 311
MspCI CTTAAG 1 cut(s) 130
MspI CCGG 3 cut(s) 234, 515, 1014
MvnI CGCG 1 cut(s) 926
MwoI GCNNNNNNNGC 2 cut(s) 146, 1019
NcoI CCATGG 1 cut(s) 789
NdeII GATC 2 cut(s) 48, 621
NlaIII CATG 7 cut(s) 140, 463, 793, 850, 916, 1281, 1318
NlaIV GGNNCC 2 cut(s) 591, 717
NmuCI GTSAC 2 cut(s) 779, 814
NspI RCATGY 2 cut(s) 140, 1281
OliI CACNNNNGTG 1 cut(s) 311
PaeI GCATGC 1 cut(s) 140
PceI AGGCCT 1 cut(s) 9
PfeI GAWTC 7 cut(s) 55, 447, 521, 632, 682, 869, 1100
PflMI CCANNNNNTGG 1 cut(s) 1163
PkrI GCNGC 1 cut(s) 1303
PleI GAGTC 1 cut(s) 127
PpsI GAGTC 1 cut(s) 127
PpuMI RGGWCCY 1 cut(s) 590
Psp5II RGGWCCY 1 cut(s) 590
PspFI CCCAGC 1 cut(s) 1256
PspN4I GGNNCC 2 cut(s) 591, 717
PspPI GGNCC 1 cut(s) 590
PspPPI RGGWCCY 1 cut(s) 590
RsaI GTAC 2 cut(s) 403, 979
RsaNI GTAC 2 cut(s) 402, 978
RseI CAYNNNNRTG 2 cut(s) 44, 311
SaqAI TTAA 5 cut(s) 131, 359, 585, 1028, 1308
SatI GCNGC 1 cut(s) 1302
Sau3AI GATC 2 cut(s) 48, 621
Sau96I GGNCC 1 cut(s) 590
SchI GAGTC 1 cut(s) 128
SfaNI GCATC 2 cut(s) 946, 998
SfcI CTRYAG 1 cut(s) 488
SinI GGWCC 1 cut(s) 590
SmiMI CAYNNNNRTG 2 cut(s) 44, 311
SmlI CTYRAG 1 cut(s) 130
SmoI CTYRAG 1 cut(s) 130
SpeI ACTAGT 1 cut(s) 404
SphI GCATGC 1 cut(s) 140
Sse9I AATT 4 cut(s) 505, 546, 1104, 1305
SseBI AGGCCT 1 cut(s) 9
SsiI CCGC 1 cut(s) 926
SspMI CTAG 2 cut(s) 398, 405
StuI AGGCCT 1 cut(s) 9
StyI CCWWGG 1 cut(s) 789
TaaI ACNGT 2 cut(s) 670, 1201
TaqI TCGA 3 cut(s) 26, 51, 503
TasI AATT 4 cut(s) 505, 546, 1104, 1305
TfiI GAWTC 7 cut(s) 55, 447, 521, 632, 682, 869, 1100
Tru1I TTAA 5 cut(s) 131, 359, 585, 1028, 1308
Tru9I TTAA 5 cut(s) 131, 359, 585, 1028, 1308
TscAI CASTG 2 cut(s) 342, 1206
TseFI GTSAC 2 cut(s) 779, 814
TseI GCWGC 1 cut(s) 1301
Tsp45I GTSAC 2 cut(s) 779, 814
TspDTI ATGAA 4 cut(s) 64, 384, 534, 1163
TspGWI ACGGA 2 cut(s) 73, 1099
TspRI CASTG 2 cut(s) 342, 1206
Van91I CCANNNNNTGG 1 cut(s) 1163
Vha464I CTTAAG 1 cut(s) 130
VpaK11BI GGWCC 1 cut(s) 590
XagI CCTNNNNNAGG 1 cut(s) 166
XapI RAATTY 1 cut(s) 505
XceI RCATGY 2 cut(s) 140, 1281
XcmI CCANNNNNNNNNTGG 1 cut(s) 967
XspI CTAG 2 cut(s) 398, 405
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.