Rh5AG530700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0013150
Physical Location & Seq
Reverse (-)
91249754 .. 91251280
1527 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG530700.1

Sequence Viewer

Length: 684 bp
ATGTTCAATCGGCTAGCGAATTTGCATATGACAGGCCTGTTCAATCTTATATCGACTTTGATTTCCATTTTGATGATGGAGAATCTGTTACAACTCTCTTTCATTCGTTCCGTGTCTCAGCTTTTCTCCGAAACTGCAACTATGGAGTCCATTTCCTTAAGGCATGCAATCTCTGCAACACATCACACCTATCTCAGGAGAACAAGAGTAAACCCAGAAAAGCCATTTCAGACAAAAACGTTTCACCTTCACAACCCAACTGGCCGTTTTCAAATCCATTCCAAACTCTCTGATTTCCAAGACTATGCAAAGCCTTCACGCCTATTGCAAGCCACAGAAGTGAATGTCTGCACAGATACGTCAGTGGAAAAAAGCTTCTTGTCATTAAGGGATTATGAATGTCAGGCTTTATATAAGGTCAAGCTAGGTACTAGTAATGCTTATGGTTCAAGTCTCTCCGACCTTACTGCTGGAATCCTCCTGTGCGTGATAGACGAAAATGGTAACTCCATACTACAGAGGATACCCTCAAATTTGAGACCGGATGAATCTAAAGAAGTAGAGGATGAGATAATTCAGTTTCAAAGAGGTTCTGTTGATGAGTTCACCTTTAAGGGACCTAAACTTGGAAAAGTTGAAGCTCTTTGGATCAGTCTTGAATCAGGTCAGGCCAATGGAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

227

Amino Acids

25.54

Weight (kDa)

6.66

Isoelectric Point (pI)

35.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7755 PF24938 136 - 223 1.5e-39 Domain of unknown function (DUF7755)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013150)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55280 AT1G55280
fragaria_vesca FvH4_3g45230
malus_domestica MD03G1007100.v1.1
prunus_persica Prupe.6G006700_v2.0.a1
pyrus_communis pycom03g00720
rosa_chinensis RchiOBHm_Chr5g0082841
rosa_laevigata RLG00000036994
rosa_multiflora Rmu_sc0001381.1_g000013 Rmu_sc0007172.1_g000002
rosa_roxburghii Rroxscaffold_1G00001590
rosa_rugosa Rorug05G0480300
rosa_samantha Rh5AG530600 Rh5AG530700 Rh5BG557600 Rh5CG580600 Rh5DG565900
rosa_wichuraiana Rw5G049800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 239
AclWI GGATC 1 cut(s) 656
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 2 cut(s) 19, 532
AfaI GTAC 1 cut(s) 430
AfiI CCNNNNNNNGG 2 cut(s) 195, 626
AflII CTTAAG 1 cut(s) 157
AgsI TTSAA 7 cut(s) 7, 43, 272, 450, 584, 638, 659
AhlI ACTAGT 1 cut(s) 431
AleI CACNNNNGTG 1 cut(s) 338
AluBI AGCT 4 cut(s) 121, 375, 424, 641
AluI AGCT 4 cut(s) 121, 375, 424, 641
Alw26I GTCTC 3 cut(s) 120, 458, 532
AlwI GGATC 1 cut(s) 656
AoxI GGCC 3 cut(s) 34, 262, 669
ApoI RAATTY 2 cut(s) 19, 532
AspS9I GGNCC 1 cut(s) 617
AsuHPI GGTGA 2 cut(s) 236, 598
AsuNHI GCTAGC 1 cut(s) 13
AvaII GGWCC 1 cut(s) 617
BaeI ACNNNNGTAYC 2 cut(s) 348, 381
BccI CCATC 1 cut(s) 70
BceAI ACGGC 1 cut(s) 249
BcgI CGANNNNNNTGC 2 cut(s) 449, 483
BciVI GTATCC 1 cut(s) 516
BcoDI GTCTC 3 cut(s) 120, 458, 532
BcuI ACTAGT 1 cut(s) 431
BfaI CTAG 3 cut(s) 14, 425, 432
BfmI CTRYAG 1 cut(s) 515
BfrI CTTAAG 1 cut(s) 157
BfuI GTATCC 1 cut(s) 516
Bme18I GGWCC 1 cut(s) 617
BmgT120I GGNCC 1 cut(s) 617
BmiI GGNNCC 1 cut(s) 618
BmtI GCTAGC 1 cut(s) 17
BsaI GGTCTC 1 cut(s) 532
BsaWI WCCGGW 1 cut(s) 541
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bsc4I CCNNNNNNNGG 2 cut(s) 195, 626
Bse1I ACTGG 1 cut(s) 265
BseGI GGATG 2 cut(s) 550, 571
BseLI CCNNNNNNNGG 2 cut(s) 195, 626
BseMII CTCAG 2 cut(s) 131, 208
BseNI ACTGG 1 cut(s) 265
BsgI GTGCAG 1 cut(s) 334
BshFI GGCC 3 cut(s) 36, 264, 671
BsiSI CCGG 1 cut(s) 542
BslFI GGGAC 1 cut(s) 630
BslI CCNNNNNNNGG 2 cut(s) 195, 626
BsmAI GTCTC 3 cut(s) 120, 458, 532
BsmFI GGGAC 1 cut(s) 630
BsnI GGCC 3 cut(s) 36, 264, 671
Bso31I GGTCTC 1 cut(s) 532
Bsp143I GATC 1 cut(s) 648
BspANI GGCC 3 cut(s) 36, 264, 671
BspCNI CTCAG 2 cut(s) 130, 207
BspLI GGNNCC 1 cut(s) 618
BspOI GCTAGC 1 cut(s) 17
BspPI GGATC 1 cut(s) 656
BspTI CTTAAG 1 cut(s) 157
BspTNI GGTCTC 1 cut(s) 532
BsrI ACTGG 1 cut(s) 265
BssMI GATC 1 cut(s) 648
BstAFI CTTAAG 1 cut(s) 157
BstAPI GCANNNNNTGC 1 cut(s) 173
BstC8I GCNNGC 3 cut(s) 15, 165, 330
BstDEI CTNAG 2 cut(s) 117, 194
BstENI CCTNNNNNAGG 1 cut(s) 193
BstF5I GGATG 2 cut(s) 550, 571
BstKTI GATC 1 cut(s) 651
BstMAI GTCTC 3 cut(s) 120, 458, 532
BstMBI GATC 1 cut(s) 648
BstMWI GCNNNNNNNGC 1 cut(s) 173
BstNSI RCATGY 1 cut(s) 167
BstSFI CTRYAG 1 cut(s) 515
BsuI GTATCC 1 cut(s) 516
BsuRI GGCC 3 cut(s) 36, 264, 671
BtsCI GGATG 2 cut(s) 550, 571
BtsIMutI CAGTG 1 cut(s) 369
Cac8I GCNNGC 3 cut(s) 15, 165, 330
Cfr13I GGNCC 1 cut(s) 617
Csp6I GTAC 1 cut(s) 429
CviAII CATG 1 cut(s) 164
CviQI GTAC 1 cut(s) 429
DdeI CTNAG 2 cut(s) 117, 194
DpnI GATC 1 cut(s) 650
DpnII GATC 1 cut(s) 648
EaeI YGGCCR 1 cut(s) 262
Eco147I AGGCCT 1 cut(s) 36
Eco31I GGTCTC 1 cut(s) 532
Eco47I GGWCC 1 cut(s) 617
EcoNI CCTNNNNNAGG 1 cut(s) 193
EcoO109I RGGNCCY 1 cut(s) 617
FaeI CATG 1 cut(s) 167
FaqI GGGAC 1 cut(s) 630
FatI CATG 1 cut(s) 163
FauNDI CATATG 1 cut(s) 27
FokI GGATG 2 cut(s) 557, 578
FspBI CTAG 3 cut(s) 14, 425, 432
HaeIII GGCC 3 cut(s) 36, 264, 671
HapII CCGG 1 cut(s) 542
Hin1II CATG 1 cut(s) 167
HindIII AAGCTT 1 cut(s) 373
HinfI GANTC 5 cut(s) 82, 146, 474, 548, 659
HpaII CCGG 1 cut(s) 542
HphI GGTGA 2 cut(s) 236, 598
Hpy166II GTNNAC 2 cut(s) 211, 606
Hpy188I TCNGA 4 cut(s) 130, 231, 292, 460
Hpy188III TCNNGA 2 cut(s) 196, 656
Hpy8I GTNNAC 2 cut(s) 211, 606
HpyAV CCTTC 2 cut(s) 257, 324
HpyCH4IV ACGT 2 cut(s) 239, 359
HpyCH4V TGCA 7 cut(s) 25, 137, 167, 176, 308, 328, 351
HpyF10VI GCNNNNNNNGC 1 cut(s) 173
HpyF3I CTNAG 2 cut(s) 117, 194
HpySE526I ACGT 2 cut(s) 239, 359
Hsp92II CATG 1 cut(s) 167
Kzo9I GATC 1 cut(s) 648
MaeI CTAG 3 cut(s) 14, 425, 432
MaeII ACGT 2 cut(s) 239, 359
MaeIII GTNAC 2 cut(s) 87, 503
MalI GATC 1 cut(s) 650
MboI GATC 1 cut(s) 648
MluCI AATT 3 cut(s) 19, 532, 573
MlyI GAGTC 1 cut(s) 155
MmeI TCCRAC 1 cut(s) 483
MnlI CCTC 6 cut(s) 488, 513, 538, 556, 581, 671
MseI TTAA 3 cut(s) 158, 386, 612
MslI CAYNNNNRTG 2 cut(s) 71, 338
MspCI CTTAAG 1 cut(s) 157
MspI CCGG 1 cut(s) 542
MwoI GCNNNNNNNGC 1 cut(s) 173
NdeI CATATG 1 cut(s) 27
NdeII GATC 1 cut(s) 648
NheI GCTAGC 1 cut(s) 13
NlaIII CATG 1 cut(s) 167
NlaIV GGNNCC 1 cut(s) 618
NspI RCATGY 1 cut(s) 167
OliI CACNNNNGTG 1 cut(s) 338
PaeI GCATGC 1 cut(s) 167
PceI AGGCCT 1 cut(s) 36
PfeI GAWTC 4 cut(s) 82, 474, 548, 659
PleI GAGTC 1 cut(s) 154
PpsI GAGTC 1 cut(s) 154
PpuMI RGGWCCY 1 cut(s) 617
Psp1406I AACGTT 1 cut(s) 239
Psp5II RGGWCCY 1 cut(s) 617
PspN4I GGNNCC 1 cut(s) 618
PspPI GGNCC 1 cut(s) 617
PspPPI RGGWCCY 1 cut(s) 617
RsaI GTAC 1 cut(s) 430
RsaNI GTAC 1 cut(s) 429
RseI CAYNNNNRTG 2 cut(s) 71, 338
SaqAI TTAA 3 cut(s) 158, 386, 612
Sau3AI GATC 1 cut(s) 648
Sau96I GGNCC 1 cut(s) 617
SchI GAGTC 1 cut(s) 155
SfcI CTRYAG 1 cut(s) 515
SinI GGWCC 1 cut(s) 617
SmiMI CAYNNNNRTG 2 cut(s) 71, 338
SmlI CTYRAG 1 cut(s) 157
SmoI CTYRAG 1 cut(s) 157
SpeI ACTAGT 1 cut(s) 431
SphI GCATGC 1 cut(s) 167
Sse9I AATT 3 cut(s) 19, 532, 573
SseBI AGGCCT 1 cut(s) 36
SspMI CTAG 3 cut(s) 14, 425, 432
StuI AGGCCT 1 cut(s) 36
TaiI ACGT 2 cut(s) 242, 362
TaqI TCGA 1 cut(s) 53
TasI AATT 3 cut(s) 19, 532, 573
TfiI GAWTC 4 cut(s) 82, 474, 548, 659
Tru1I TTAA 3 cut(s) 158, 386, 612
Tru9I TTAA 3 cut(s) 158, 386, 612
TscAI CASTG 1 cut(s) 369
TspDTI ATGAA 3 cut(s) 91, 411, 561
TspGWI ACGGA 1 cut(s) 100
TspRI CASTG 1 cut(s) 369
Vha464I CTTAAG 1 cut(s) 157
VpaK11BI GGWCC 1 cut(s) 617
XagI CCTNNNNNAGG 1 cut(s) 193
XapI RAATTY 2 cut(s) 19, 532
XceI RCATGY 1 cut(s) 167
XcmI CCANNNNNNNNNTGG 1 cut(s) 73
XspI CTAG 3 cut(s) 14, 425, 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.