Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0008737

Physical Location & Seq

Genomic Coordinates Reverse (-)

1598082 .. 1601047

Gene Structure 2966 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1020800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1815 bp

ATGGGTGACGTCGGAGAGATTGGACCTGATATCAGTTCGGATATAGAAGAAGACTTGAGGTGCAATAATATTGCGGAGAAAGATGTCAGTGACGAAGAGATTGAATCGGAAGATTTGGAGATGCGGATGTGGAAGGATCGAATCAAACTCAAAAGGCTTAGAGAAAGACAGAAACTTGAAGCTCAACAAGCTGCTGAAAAACAGAAGCCCAAGCAGACCTCTGATCAGGCTCGTAGGAAGAAAATGTCAAGAGCACAAGATGGGATTCTTAAGTATATGCTGAAGCTGATGGAAGTGTGCAAAGCTCGTGGTTTTGTGTATGGTATCATTCCTGAGAAGGGCAAGCCAGTGAGCGGTGCTTCAGATAACGTCAGGGCTTGGTGGAAAGAAAAGGTGAAGTTCGATAAGAATGGGCCTGCAGCCATAGCCAAGTATGAAGCGGAGTGTCTTGCCATGAGTGATGCAGATAATAATCGAAATGGGAATTCTCAGAGTATCCTCCAAGATCTACAAGATGCAACTCTTGGTTCTCTGTTGTCTTCTTTGATGCAACACTGTGACCCCCCTCAAAGGAAGTATCCGTTAGAAAAGGGAGTTCCACCACCTTGGTGGCCAACAGGAAATGAAGATTGGTGGGTCAAATTAGGGCTAGCCCATGGTCAGAGTCCTCCTTATAAGAAGCCGCACGACCTAAAGAAGATGTGGAAAGTTGGAGTGCTAACTGCTGTGATAAAGCATATGTCACCTGATATTGCAAAGATAAGGAAGCACGTCCGCCAGTCAAAATGCTTACAGGATAAGATGACAGCAAAGGAGAGTGCAATTTGGTTAGGGGTTTTAAGCCGAGAAGAATCTCTCATTAGGCAGCCTAGTAGTGATAATGGGACATCTGGCATTATTGAGATGCCACAAAGTGGCTGTGGTGAAAAGCAAGCTGCTGCTAGCAGTAACAGTGACTATGATGTTGATGGTACTGATGATGGGTTAGGGTCGGTTTCTTCATCCAAAGATGATATGAGGAATCAGGTGACGGATGTAGAGCCATTGAGCAAACTACGCAATAATTCTCGTAATAATGTCCAATATAAAGAGCAAGGTGAAAAGAAACCGAGGACAAAAAGGGCACGTGTCAGAGCAAGCCCTGTCGAACAACTTCCTGCACCATCTCCCAATGAGCATCTACATGTTCGAGGTGCATTGCCTGATATAAACCACACTGATGTACAGATGATTGGATTTCAGGTCCATGAGAATCAACAGGAAAATGGTGGCATTACAACTTTACGGCCGCTGGAGAACGATCTTGATGTCCAAGCACAAATACCTGCGCCTGAGTTTAACTACTACCCTGGTGTACCTTCTGAGAATGTAATTATGACACAGGGTATGCATGTTGGTGGAACACCCTTGCTTTATCATGGGGTGCAAGATGCTGACATGCATCATGGAGATACATTTAACCAGCATCATGGAGATACATTTAACCTCTATAATCCTTTGGCACAATATCCCCCAAGTCAAGACCAGCAGCTGCCTCAGATTGTAATGAATAAACCGCAGGTTATACCAACGAATGGAATCCATGTACCAGTACATAGAAATGGAAGTGAGATTGCTGGAGGAGATCCAAATTTTGTTCAAGACACATTTCAGAGTGAGCAAGACAGAACTGCCAATGCTAACTTTGGGTCTCCAATTGATAGCCTGTCGTTAGATTATGGATTATTCAACAGCCCATTCAACTTTGGAATTGATAGCACCGGTTCATTAGATGATCTTGAACTCGAAGAAATGATGGAGTACTTTGCGGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0035556 GO:0042221 GO:0042762 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071369 GO:0071495 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

EIN3

Protein Analysis

605

Amino Acids

67.49

Weight (kDa)

5.54

Isoelectric Point (pI)

52.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EIN3_DNA-bd	PF04873	37 - 284	2.9e-128	Ethylene insensitive 3, DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008737)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G73730 AT1G73730
fragaria_vesca	FvH4_3g43580 FvH4_3g43580
malus_domestica	MD03G1020800.v1.1 MD11G1022400.v1.1
prunus_persica	Prupe.6G018200_v2.0.a1 Prupe.6G018200_v2.0.a1
pyrus_communis	pycom03g01780 pycom11g01800
rosa_chinensis	RchiOBHm_Chr5g0078151
rosa_laevigata	RLG00000036758
rosa_multiflora	Rmu_sc0018349.1_g000004 Rmu_sc0018349.1_g000007
rosa_roxburghii	Rroxscaffold_1G00003980
rosa_rugosa	Rorug05G0459100 Rorug05G0459200 Rorug05G0459300
rosa_samantha	Rh5AG513600 Rh5BG536600 Rh5CG560000 Rh5DG548200
rosa_wichuraiana	Rw5G047660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	675
AatII	GACGTC	1 cut(s)	12
Acc36I	ACCTGC	2 cut(s)	1333, 1549
AccB7I	CCANNNNNTGG	2 cut(s)	914, 1574
AccBSI	CCGCTC	1 cut(s)	354
AciI	CCGC	9 cut(s)	74, 124, 354, 440, 683, 775, 1289, 1556, 1808
AclWI	GGATC	2 cut(s)	144, 1619
AcoI	YGGCCR	2 cut(s)	611, 1286
AcsI	RAATTY	2 cut(s)	484, 1630
AcuI	CTGAAG	2 cut(s)	302, 345
AcvI	CACGTG	1 cut(s)	1127
AcyI	GRCGYC	1 cut(s)	9
AdeI	CACNNNGTG	1 cut(s)	914
AfaI	GTAC	6 cut(s)	973, 1224, 1356, 1587, 1593, 1802
AfiI	CCNNNNNNNGG	5 cut(s)	338, 353, 570, 914, 1574
AflII	CTTAAG	1 cut(s)	269
AflIII	ACRYGT	2 cut(s)	1126, 1183
AgeI	ACCGGT	1 cut(s)	1760
AgsI	TTSAA	6 cut(s)	104, 179, 1640, 1729, 1741, 1781
AjiI	CACGTC	1 cut(s)	772
AjnI	CCWGG	1 cut(s)	1348
AjuI	GAANNNNNNNTTGG	2 cut(s)	613, 645
AleI	CACNNNNGTG	1 cut(s)	607
AluBI	AGCT	6 cut(s)	182, 191, 286, 305, 935, 1531
AluI	AGCT	6 cut(s)	182, 191, 286, 305, 935, 1531
Alw21I	GWGCWC	1 cut(s)	256
Alw26I	GTCTC	1 cut(s)	1695
AlwI	GGATC	2 cut(s)	144, 1619
AlwNI	CAGNNNCTG	1 cut(s)	1531
AoxI	GGCC	3 cut(s)	413, 611, 1286
ApeKI	GCWGC	7 cut(s)	191, 419, 865, 935, 938, 1528, 1531
ApoI	RAATTY	2 cut(s)	484, 1630
AsiGI	ACCGGT	1 cut(s)	1760
Asp700I	GAANNNNTTC	1 cut(s)	1152
AspLEI	GCGC	1 cut(s)	1330
AspS9I	GGNCC	3 cut(s)	23, 413, 1243
AsuHPI	GGTGA	6 cut(s)	17, 406, 735, 935, 1039, 1109
AsuNHI	GCTAGC	2 cut(s)	649, 941
AvaII	GGWCC	2 cut(s)	23, 1243
BaeGI	GKGCMC	1 cut(s)	1126
BalI	TGGCCA	1 cut(s)	613
BauI	CACGAG	1 cut(s)	306
BbrPI	CACGTG	1 cut(s)	1127
BbsI	GAAGAC	2 cut(s)	57, 531
Bbv12I	GWGCWC	1 cut(s)	256
BbvI	GCAGC	7 cut(s)	178, 431, 877, 922, 925, 1518, 1540
BccI	CCATC	6 cut(s)	254, 283, 962, 974, 1171, 1789
BceAI	ACGGC	1 cut(s)	1301
BciT130I	CCWGG	1 cut(s)	1350
BciVI	GTATCC	2 cut(s)	506, 588
BclI	TGATCA	1 cut(s)	223
BcoDI	GTCTC	1 cut(s)	1695
BfaI	CTAG	3 cut(s)	650, 870, 942
BfmI	CTRYAG	1 cut(s)	417
BfrI	CTTAAG	1 cut(s)	269
BfuAI	ACCTGC	2 cut(s)	1333, 1549
BfuI	GTATCC	2 cut(s)	506, 588
BglII	AGATCT	1 cut(s)	505
BmcAI	AGTACT	1 cut(s)	1802
Bme1390I	CCNGG	1 cut(s)	1350
Bme18I	GGWCC	2 cut(s)	23, 1243
BmgBI	CACGTC	1 cut(s)	772
BmgT120I	GGNCC	3 cut(s)	23, 413, 1243
BmrFI	CCNGG	1 cut(s)	1350
BmsI	GCATC	9 cut(s)	111, 451, 505, 537, 894, 1186, 1420, 1450, 1474
BmtI	GCTAGC	2 cut(s)	653, 945
BpiI	GAAGAC	2 cut(s)	57, 531
BpmI	CTGGAG	2 cut(s)	1313, 1638
BpuEI	CTTGAG	1 cut(s)	76
BsaAI	YACGTR	1 cut(s)	1127
BsaBI	GATNNNNATC	1 cut(s)	471
BsaHI	GRCGYC	1 cut(s)	9
BsaI	GGTCTC	1 cut(s)	1695
BsaJI	CCNNGG	4 cut(s)	605, 655, 1109, 1348
BsaWI	WCCGGW	1 cut(s)	1760
Bsc4I	CCNNNNNNNGG	5 cut(s)	338, 353, 570, 914, 1574
Bse118I	RCCGGY	1 cut(s)	1760
Bse1I	ACTGG	3 cut(s)	347, 778, 1589
Bse3DI	GCAATG	1 cut(s)	1196
Bse8I	GATNNNNATC	1 cut(s)	471
BseBI	CCWGG	1 cut(s)	1350
BseDI	CCNNGG	4 cut(s)	605, 655, 1109, 1348
BseGI	GGATG	3 cut(s)	132, 1001, 1039
BseJI	GATNNNNATC	1 cut(s)	471
BseLI	CCNNNNNNNGG	5 cut(s)	338, 353, 570, 914, 1574
BseMI	GCAATG	1 cut(s)	1196
BseMII	CTCAG	5 cut(s)	324, 503, 1323, 1353, 1550
BseNI	ACTGG	3 cut(s)	347, 778, 1589
BseRI	GAGGAG	1 cut(s)	1635
BseSI	GKGCMC	1 cut(s)	1126
BseX3I	CGGCCG	1 cut(s)	1286
BseXI	GCAGC	7 cut(s)	178, 431, 877, 922, 925, 1518, 1540
BsgI	GTGCAG	1 cut(s)	1143
Bsh1285I	CGRYCG	1 cut(s)	1289
BshFI	GGCC	3 cut(s)	415, 613, 1288
BshTI	ACCGGT	1 cut(s)	1760
BsiEI	CGRYCG	1 cut(s)	1289
BsiHKAI	GWGCWC	1 cut(s)	256
BsiSI	CCGG	1 cut(s)	1761
BslFI	GGGAC	1 cut(s)	898
BslI	CCNNNNNNNGG	5 cut(s)	338, 353, 570, 914, 1574
BsmAI	GTCTC	1 cut(s)	1695
BsmFI	GGGAC	1 cut(s)	898
BsnI	GGCC	3 cut(s)	415, 613, 1288
Bso31I	GGTCTC	1 cut(s)	1695
Bsp1286I	GDGCHC	2 cut(s)	256, 1126
Bsp1407I	TGTACA	1 cut(s)	1222
Bsp143I	GATC	6 cut(s)	136, 223, 505, 1300, 1624, 1774
Bsp19I	CCATGG	1 cut(s)	655
BspACI	CCGC	9 cut(s)	74, 124, 354, 440, 683, 775, 1289, 1556, 1808
BspANI	GGCC	3 cut(s)	415, 613, 1288
BspCNI	CTCAG	5 cut(s)	325, 502, 1324, 1354, 1549
BspMAI	CTGCAG	1 cut(s)	421
BspMI	ACCTGC	2 cut(s)	1333, 1549
BspOI	GCTAGC	2 cut(s)	653, 945
BspPI	GGATC	2 cut(s)	144, 1619
BspTI	CTTAAG	1 cut(s)	269
BspTNI	GGTCTC	1 cut(s)	1695
BsrBI	CCGCTC	1 cut(s)	354
BsrDI	GCAATG	1 cut(s)	1196
BsrFI	RCCGGY	1 cut(s)	1760
BsrGI	TGTACA	1 cut(s)	1222
BsrI	ACTGG	3 cut(s)	347, 778, 1589
BssAI	RCCGGY	1 cut(s)	1760
BssECI	CCNNGG	4 cut(s)	605, 655, 1109, 1348
BssMI	GATC	6 cut(s)	136, 223, 505, 1300, 1624, 1774
BssNI	GRCGYC	1 cut(s)	9
BssSI	CACGAG	1 cut(s)	306
BssT1I	CCWWGG	2 cut(s)	605, 655
Bst2BI	CACGAG	1 cut(s)	306
Bst2UI	CCWGG	1 cut(s)	1350
Bst4CI	ACNGT	2 cut(s)	557, 953
Bst6I	CTCTTC	1 cut(s)	90
BstACI	GRCGYC	1 cut(s)	9
BstAFI	CTTAAG	1 cut(s)	269
BstAUI	TGTACA	1 cut(s)	1222
BstBAI	YACGTR	1 cut(s)	1127
BstC8I	GCNNGC	6 cut(s)	344, 417, 651, 933, 943, 1138
BstDEI	CTNAG	6 cut(s)	158, 333, 489, 1332, 1362, 1536
BstDSI	CCRYGG	1 cut(s)	655
BstF5I	GGATG	3 cut(s)	132, 1001, 1039
BstHHI	GCGC	1 cut(s)	1330
BstKTI	GATC	6 cut(s)	139, 226, 508, 1303, 1627, 1777
BstMAI	GTCTC	1 cut(s)	1695
BstMBI	GATC	6 cut(s)	136, 223, 505, 1300, 1624, 1774
BstMCI	CGRYCG	1 cut(s)	1289
BstMWI	GCNNNNNNNGC	3 cut(s)	188, 425, 1056
BstNI	CCWGG	1 cut(s)	1350
BstNSI	RCATGY	3 cut(s)	1187, 1394, 1441
BstSCI	CCNGG	1 cut(s)	1348
BstSFI	CTRYAG	1 cut(s)	417
BstSLI	GKGCMC	1 cut(s)	1126
BstV1I	GCAGC	7 cut(s)	178, 431, 877, 922, 925, 1518, 1540
BstV2I	GAAGAC	2 cut(s)	57, 531
BstX2I	RGATCY	2 cut(s)	505, 1624
BstXI	CCANNNNNNTGG	3 cut(s)	606, 609, 1469
BstYI	RGATCY	2 cut(s)	505, 1624
BstZI	CGGCCG	1 cut(s)	1286
BsuI	GTATCC	2 cut(s)	506, 588
BsuRI	GGCC	3 cut(s)	415, 613, 1288
BtgI	CCRYGG	1 cut(s)	655
BtrI	CACGTC	1 cut(s)	772
BtsCI	GGATG	3 cut(s)	132, 1001, 1039
BtsIMutI	CAGTG	5 cut(s)	94, 354, 553, 958, 1215
BveI	ACCTGC	2 cut(s)	1333, 1549
Cac8I	GCNNGC	6 cut(s)	344, 417, 651, 933, 943, 1138
CaiI	CAGNNNCTG	1 cut(s)	1531
CfoI	GCGC	1 cut(s)	1330
Cfr10I	RCCGGY	1 cut(s)	1760
Cfr13I	GGNCC	3 cut(s)	23, 413, 1243
Csp6I	GTAC	6 cut(s)	972, 1223, 1355, 1586, 1592, 1801
CspAI	ACCGGT	1 cut(s)	1760
CspCI	CAANNNNNGTGG	2 cut(s)	289, 324
CviQI	GTAC	6 cut(s)	972, 1223, 1355, 1586, 1592, 1801
DdeI	CTNAG	6 cut(s)	158, 333, 489, 1332, 1362, 1536
DpnI	GATC	6 cut(s)	138, 225, 507, 1302, 1626, 1776
DpnII	GATC	6 cut(s)	136, 223, 505, 1300, 1624, 1774
DraIII	CACNNNGTG	1 cut(s)	914
EaeI	YGGCCR	2 cut(s)	611, 1286
EagI	CGGCCG	1 cut(s)	1286
Eam1104I	CTCTTC	1 cut(s)	90
EarI	CTCTTC	1 cut(s)	90
EciI	GGCGGA	1 cut(s)	764
EclXI	CGGCCG	1 cut(s)	1286
Eco130I	CCWWGG	2 cut(s)	605, 655
Eco31I	GGTCTC	1 cut(s)	1695
Eco32I	GATATC	1 cut(s)	31
Eco47I	GGWCC	2 cut(s)	23, 1243
Eco52I	CGGCCG	1 cut(s)	1286
Eco57I	CTGAAG	2 cut(s)	302, 345
Eco72I	CACGTG	1 cut(s)	1127
EcoRI	GAATTC	1 cut(s)	484
EcoRII	CCWGG	1 cut(s)	1348
EcoRV	GATATC	1 cut(s)	31
EcoT14I	CCWWGG	2 cut(s)	605, 655
EcoT22I	ATGCAT	2 cut(s)	1392, 1443
ErhI	CCWWGG	2 cut(s)	605, 655
FaqI	GGGAC	1 cut(s)	898
FauNDI	CATATG	1 cut(s)	738
FbaI	TGATCA	1 cut(s)	223
FokI	GGATG	3 cut(s)	139, 988, 1046
FspBI	CTAG	3 cut(s)	650, 870, 942
GlaI	GCGC	1 cut(s)	1329
GsuI	CTGGAG	2 cut(s)	1313, 1638
HaeIII	GGCC	3 cut(s)	415, 613, 1288
HapII	CCGG	1 cut(s)	1761
HhaI	GCGC	1 cut(s)	1330
Hin1I	GRCGYC	1 cut(s)	9
Hin6I	GCGC	1 cut(s)	1328
HinP1I	GCGC	1 cut(s)	1328
HinfI	GANTC	8 cut(s)	104, 141, 265, 664, 851, 1021, 1252, 1578
HpaII	CCGG	1 cut(s)	1761
HphI	GGTGA	6 cut(s)	17, 406, 735, 935, 1039, 1109
Hpy166II	GTNNAC	1 cut(s)	1355
Hpy188III	TCNNGA	6 cut(s)	249, 332, 1304, 1520, 1640, 1778
Hpy8I	GTNNAC	1 cut(s)	1355
Hpy99I	CGWCG	1 cut(s)	14
HpyAV	CCTTC	3 cut(s)	127, 331, 1368
HpyCH4III	ACNGT	2 cut(s)	557, 953
HpyCH4IV	ACGT	4 cut(s)	9, 369, 771, 1126
HpyF10VI	GCNNNNNNNGC	3 cut(s)	188, 425, 1056
HpyF3I	CTNAG	6 cut(s)	158, 333, 489, 1332, 1362, 1536
HpySE526I	ACGT	4 cut(s)	9, 369, 771, 1126
Hsp92I	GRCGYC	1 cut(s)	9
HspAI	GCGC	1 cut(s)	1328
Ksp22I	TGATCA	1 cut(s)	223
Kzo9I	GATC	6 cut(s)	136, 223, 505, 1300, 1624, 1774
Lsp1109I	GCAGC	7 cut(s)	178, 431, 877, 922, 925, 1518, 1540
LweI	GCATC	9 cut(s)	111, 451, 505, 537, 894, 1186, 1420, 1450, 1474
MaeI	CTAG	3 cut(s)	650, 870, 942
MaeII	ACGT	4 cut(s)	9, 369, 771, 1126
MaeIII	GTNAC	7 cut(s)	5, 89, 557, 741, 947, 953, 1027
MalI	GATC	6 cut(s)	138, 225, 507, 1302, 1626, 1776
MbiI	CCGCTC	1 cut(s)	354
MboI	GATC	6 cut(s)	136, 223, 505, 1300, 1624, 1774
MfeI	CAATTG	1 cut(s)	1695
MflI	RGATCY	2 cut(s)	505, 1624
MhlI	GDGCHC	2 cut(s)	256, 1126
MlsI	TGGCCA	1 cut(s)	613
MluCI	AATT	8 cut(s)	484, 641, 822, 1063, 1371, 1630, 1695, 1749
MluNI	TGGCCA	1 cut(s)	613
MlyI	GAGTC	1 cut(s)	673
MmeI	TCCRAC	1 cut(s)	691
Mox20I	TGGCCA	1 cut(s)	613
Mph1103I	ATGCAT	2 cut(s)	1392, 1443
MroXI	GAANNNNTTC	1 cut(s)	1152
MscI	TGGCCA	1 cut(s)	613
MseI	TTAA	5 cut(s)	270, 839, 1338, 1458, 1482
MslI	CAYNNNNRTG	5 cut(s)	607, 1182, 1218, 1395, 1599
Msp20I	TGGCCA	1 cut(s)	613
MspA1I	CMGCKG	2 cut(s)	1291, 1531
MspCI	CTTAAG	1 cut(s)	269
MspI	CCGG	1 cut(s)	1761
MspR9I	CCNGG	1 cut(s)	1350
MunI	CAATTG	1 cut(s)	1695
MvaI	CCWGG	1 cut(s)	1350
MwoI	GCNNNNNNNGC	3 cut(s)	188, 425, 1056
NcoI	CCATGG	1 cut(s)	655
NdeI	CATATG	1 cut(s)	738
NdeII	GATC	6 cut(s)	136, 223, 505, 1300, 1624, 1774
NheI	GCTAGC	2 cut(s)	649, 941
NmeAIII	GCCGAG	1 cut(s)	869
NmuCI	GTSAC	6 cut(s)	5, 89, 557, 741, 953, 1027
NsiI	ATGCAT	2 cut(s)	1392, 1443
NspI	RCATGY	3 cut(s)	1187, 1394, 1441
OliI	CACNNNNGTG	1 cut(s)	607
PciI	ACATGT	1 cut(s)	1183
PdmI	GAANNNNTTC	1 cut(s)	1152
PfeI	GAWTC	7 cut(s)	104, 141, 265, 851, 1021, 1252, 1578
PflMI	CCANNNNNTGG	2 cut(s)	914, 1574
PinAI	ACCGGT	1 cut(s)	1760
PleI	GAGTC	1 cut(s)	672
PmaCI	CACGTG	1 cut(s)	1127
PmlI	CACGTG	1 cut(s)	1127
PpsI	GAGTC	1 cut(s)	672
Ppu21I	YACGTR	1 cut(s)	1127
PscI	ACATGT	1 cut(s)	1183
PsiI	TTATAA	1 cut(s)	675
Psp6I	CCWGG	1 cut(s)	1348
PspCI	CACGTG	1 cut(s)	1127
PspGI	CCWGG	1 cut(s)	1348
PspPI	GGNCC	3 cut(s)	23, 413, 1243
PstI	CTGCAG	1 cut(s)	421
PstNI	CAGNNNCTG	1 cut(s)	1531
PsuI	RGATCY	2 cut(s)	505, 1624
PvuII	CAGCTG	1 cut(s)	1531
RsaI	GTAC	6 cut(s)	973, 1224, 1356, 1587, 1593, 1802
RsaNI	GTAC	6 cut(s)	972, 1223, 1355, 1586, 1592, 1801
RseI	CAYNNNNRTG	5 cut(s)	607, 1182, 1218, 1395, 1599
SaqAI	TTAA	5 cut(s)	270, 839, 1338, 1458, 1482
Sau3AI	GATC	6 cut(s)	136, 223, 505, 1300, 1624, 1774
Sau96I	GGNCC	3 cut(s)	23, 413, 1243
ScaI	AGTACT	1 cut(s)	1802
SchI	GAGTC	1 cut(s)	673
ScrFI	CCNGG	1 cut(s)	1350
SduI	GDGCHC	2 cut(s)	256, 1126
SfaNI	GCATC	9 cut(s)	111, 451, 505, 537, 894, 1186, 1420, 1450, 1474
SfcI	CTRYAG	1 cut(s)	417
SinI	GGWCC	2 cut(s)	23, 1243
SmiMI	CAYNNNNRTG	5 cut(s)	607, 1182, 1218, 1395, 1599
SmlI	CTYRAG	2 cut(s)	55, 269
SmoI	CTYRAG	2 cut(s)	55, 269
Sse9I	AATT	8 cut(s)	484, 641, 822, 1063, 1371, 1630, 1695, 1749
SsiI	CCGC	9 cut(s)	74, 124, 354, 440, 683, 775, 1289, 1556, 1808
SspI	AATATT	1 cut(s)	70
SspMI	CTAG	3 cut(s)	650, 870, 942
StyD4I	CCNGG	1 cut(s)	1348
StyI	CCWWGG	2 cut(s)	605, 655
TaaI	ACNGT	2 cut(s)	557, 953
TaiI	ACGT	4 cut(s)	12, 372, 774, 1129
TaqI	TCGA	6 cut(s)	139, 402, 475, 1146, 1189, 1785
TasI	AATT	8 cut(s)	484, 641, 822, 1063, 1371, 1630, 1695, 1749
TatI	WGTACW	3 cut(s)	1222, 1591, 1800
TauI	GCSGC	3 cut(s)	685, 1291, 1811
TfiI	GAWTC	7 cut(s)	104, 141, 265, 851, 1021, 1252, 1578
Tru1I	TTAA	5 cut(s)	270, 839, 1338, 1458, 1482
Tru9I	TTAA	5 cut(s)	270, 839, 1338, 1458, 1482
TscAI	CASTG	5 cut(s)	94, 354, 560, 958, 1222
TseFI	GTSAC	6 cut(s)	5, 89, 557, 741, 953, 1027
TseI	GCWGC	7 cut(s)	191, 419, 865, 935, 938, 1528, 1531
Tsp45I	GTSAC	6 cut(s)	5, 89, 557, 741, 953, 1027
TspDTI	ATGAA	5 cut(s)	450, 639, 990, 1562, 1755
TspGWI	ACGGA	2 cut(s)	570, 1046
TspRI	CASTG	5 cut(s)	94, 354, 560, 958, 1222
Van91I	CCANNNNNTGG	2 cut(s)	914, 1574
Vha464I	CTTAAG	1 cut(s)	269
VpaK11BI	GGWCC	2 cut(s)	23, 1243
XapI	RAATTY	2 cut(s)	484, 1630
XceI	RCATGY	3 cut(s)	1187, 1394, 1441
XcmI	CCANNNNNNNNNTGG	1 cut(s)	606
XmnI	GAANNNNTTC	1 cut(s)	1152
XspI	CTAG	3 cut(s)	650, 870, 942
ZraI	GACGTC	1 cut(s)	10
ZrmI	AGTACT	1 cut(s)	1802
Zsp2I	ATGCAT	2 cut(s)	1392, 1443

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.