Rh5AG513600

ETHYLENE INSENSITIVE 3-like 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0008737
Physical Location & Seq
Forward (+)
86821893 .. 86824300
2408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG513600.1

Sequence Viewer

Length: 1818 bp
ATGGGTGACCTTGGGGAGGTTGGACCTGAAATTAGTTCGGATATAGAAGAGGACTTGAGGTGTGATAATTTAGTGGAGAAAGATGTCAGTGATGAGGAGATTGATCCGGAAGAGCTGGAGAGACGGATGTGGAAGGATCGAATCAAACTCAAAAGGATCAAAGAAAAACAGAAAATTGAGGCTCAACAAGCTGCAGAAAGGCAGAAGCCCAAACAGACCACTGATCAGGCTCGAAGGAAGAAAATGTCAAGAGCACAAGATGGAATTCTAAAGTATATGTTGAAGCTGATGGAAGTGTGTCAAGCTCGTGGATTTGTGTATGGTATCATTCCTGAGAAGGGCAAGCCAGTAAGCGGTGCTTCTGATAACATCAGAGCATGGTGGAAAGAAAAAGTGAAGTTTGATAAGAATGGCCCTGCAGCCATAGACAAGTATGAAGCAGAGATTCTTGCCATGACTGATGCAGACAATAACCGAAATGGTAATTCCCAGACCATCCTCCAAGATCTACAAGATGCAACTCTTGGTTCTCTACTATCTTCATTGATGCAACATTGCGACCCCCCTCAAAGGAAGTATCCATTAGAAAAGGCAGTTCCGCCTCCTTGGTGGCCGACAGGAAATGAAGATTGGTGGATGAAATCAGGGTTACCCTGTGGTCAGAGTCCTCCATATAAGAAGCCACATGACTTAAAGAAGATGTGGAAAGTTGGGGTGTTAACAGCTGTGATAAAGCACATGTCCCCTGATATTGCAAAGATAAGGCGGCATGTCCGTCAGTCAAAATGCTTACAGGATAAGATGACAGCAAAGGAGAGTGCAATTTGGTTGGGGGTTCTAAGTCGAGAAGAAGCCCTCATTCGACAACCCAGTAGCGATAATGGGACATCTGGCATAACTGAGATGCCACGTCGTGGCCGCGGTGAAAAGCATGCTACTGCTAGTAGTAACAGTGATTATGATGTTGATGGTACTGATAAGGAGCTAATCGATGCAGGATCTGTTTCATCCAAAGATGACAGGAGGACTGAGCTGATGGATGTAGAGCCTTCTAGCAATCGACGCAGTAGTACTCCTACTAATCATGTCCAAGATAAAGGGAGAGGTGAAAAGCCAAGAAAGAGAAAAAGGGCTCCTGTAAGACCAAGCTCTGATGATAAACTTCCTGCACCAAGTCACAATGAGCCTTTGCATGTTGAACCATTAAATGCTTTGCCTGATATAAACCACACTGATGCACAGATGATTGGATTTCCAATTCATGAAAATCAACAGGAAAATGTTTCAGTCACAACTTTACGGCCACCAGAGCAAGATCTTGATGTCCAAGCATTACCGGCGTCTGAGTTTAACTACTATGCTGATGTACCTCCTGATGGTGTAACTGCGACGACACAGGGCATGCATGTGGGTGGAACACCGCTGCTTTATCATGGGATGCAAAGTGCTGAGATGCATCATGGAAATATGTATAACCTTTATAATCCATCAGCAGAACATGTGCCCAGTCATGATAGGCAGCTGTCTCAGATTGGCATGAATGAACTGCAAATTGGACCAGCAGATGTTGTCCCTTTACAAACTATAAGAAATGGAAATGAGATTACTGGAGGAGATATGCCATTCTATGCAAAAGACCCATTTCAGAGTGAGCAAGACAGAACTGTAGATGCTAACTTTGGCTCTCCAATTGACAGCCTGTCATTAGATTATGGGGGACTATTTAACAGCCCATTCCGACTTGACCTTGGAATTGAGGGCACTGGTTCATTAGATGACCTGAATGTGGATGAAATGATGGCATACTTTGCAGCATAA

Protein Analysis

605

Amino Acids

67.7

Weight (kDa)

5.51

Isoelectric Point (pI)

60.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EIN3_DNA-bd PF04873 37 - 284 7.6e-127 Ethylene insensitive 3, DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1482
AccB7I CCANNNNNTGG 1 cut(s) 914
AccII CGCG 1 cut(s) 921
AccIII TCCGGA 1 cut(s) 106
AciI CCGC 6 cut(s) 354, 599, 766, 919, 921, 1421
AclWI GGATC 4 cut(s) 98, 144, 164, 1006
AcoI YGGCCR 3 cut(s) 611, 916, 1301
AcsI RAATTY 1 cut(s) 264
AcyI GRCGYC 1 cut(s) 1340
AdeI CACNNNGTG 1 cut(s) 914
AfaI GTAC 3 cut(s) 973, 1072, 1368
AfiI CCNNNNNNNGG 7 cut(s) 16, 338, 353, 570, 914, 1376, 1786
AflIII ACRYGT 2 cut(s) 738, 1498
AgsI TTSAA 2 cut(s) 283, 1199
AhdI GACNNNNNGTC 1 cut(s) 1699
AjiI CACGTC 1 cut(s) 911
AjuI GAANNNNNNNTTGG 4 cut(s) 613, 645, 1536, 1568
AluBI AGCT 9 cut(s) 115, 191, 286, 305, 725, 985, 1033, 1149, 1522
AluI AGCT 9 cut(s) 115, 191, 286, 305, 725, 985, 1033, 1149, 1522
Alw21I GWGCWC 1 cut(s) 256
Alw26I GTCTC 2 cut(s) 115, 1530
AlwI GGATC 4 cut(s) 98, 144, 164, 1006
AlwNI CAGNNNCTG 1 cut(s) 1001
Aor13HI TCCGGA 1 cut(s) 106
AoxI GGCC 4 cut(s) 412, 611, 916, 1301
ApeKI GCWGC 5 cut(s) 191, 419, 1423, 1519, 1811
ApoI RAATTY 1 cut(s) 264
AspS9I GGNCC 3 cut(s) 23, 413, 1556
AsuHPI GGTGA 3 cut(s) 17, 935, 1118
AvaII GGWCC 2 cut(s) 23, 1556
BaeGI GKGCMC 2 cut(s) 1506, 1763
BanII GRGCYC 1 cut(s) 1135
BauI CACGAG 1 cut(s) 306
Bbv12I GWGCWC 1 cut(s) 256
BbvI GCAGC 4 cut(s) 178, 431, 1410, 1531
BccI CCATC 8 cut(s) 254, 283, 503, 962, 1030, 1370, 1495, 1792
BceAI ACGGC 1 cut(s) 1316
BciVI GTATCC 1 cut(s) 588
BclI TGATCA 1 cut(s) 223
BcoDI GTCTC 2 cut(s) 115, 1530
BfaI CTAG 2 cut(s) 942, 1053
BfmI CTRYAG 3 cut(s) 192, 417, 1665
BfuI GTATCC 1 cut(s) 588
BglII AGATCT 2 cut(s) 505, 1315
BisI GCNGC 7 cut(s) 192, 420, 767, 919, 1424, 1520, 1812
BlsI GCNGC 7 cut(s) 193, 421, 768, 920, 1425, 1521, 1813
BmcAI AGTACT 1 cut(s) 1072
Bme18I GGWCC 2 cut(s) 23, 1556
BmeRI GACNNNNNGTC 1 cut(s) 1699
BmgBI CACGTC 1 cut(s) 911
BmgT120I GGNCC 3 cut(s) 23, 413, 1556
BmiI GGNNCC 1 cut(s) 1134
BmrI ACTGGG 2 cut(s) 864, 1500
BmuI ACTGGG 2 cut(s) 864, 1500
BpmI CTGGAG 2 cut(s) 137, 1629
BpuEI CTTGAG 1 cut(s) 76
Bsa29I ATCGAT 1 cut(s) 990
BsaHI GRCGYC 1 cut(s) 1340
BsaJI CCNNGG 4 cut(s) 10, 605, 919, 1747
BsaWI WCCGGW 1 cut(s) 106
Bsc4I CCNNNNNNNGG 7 cut(s) 16, 338, 353, 570, 914, 1376, 1786
Bse118I RCCGGY 1 cut(s) 1336
Bse1I ACTGG 5 cut(s) 347, 870, 1506, 1612, 1768
Bse3DI GCAATG 1 cut(s) 553
BseAI TCCGGA 1 cut(s) 106
BseCI ATCGAT 1 cut(s) 990
BseDI CCNNGG 4 cut(s) 10, 605, 919, 1747
BseGI GGATG 7 cut(s) 132, 495, 642, 1007, 1045, 1443, 1795
BseLI CCNNNNNNNGG 7 cut(s) 16, 338, 353, 570, 914, 1376, 1786
BseMI GCAATG 1 cut(s) 553
BseMII CTCAG 6 cut(s) 324, 891, 1020, 1335, 1440, 1541
BseNI ACTGG 5 cut(s) 347, 870, 1506, 1612, 1768
BseRI GAGGAG 2 cut(s) 110, 1626
BseSI GKGCMC 2 cut(s) 1506, 1763
BseXI GCAGC 4 cut(s) 178, 431, 1410, 1531
BsgI GTGCAG 1 cut(s) 1152
Bsh1236I CGCG 1 cut(s) 921
BshFI GGCC 4 cut(s) 414, 613, 918, 1303
BshVI ATCGAT 1 cut(s) 990
BsiHKAI GWGCWC 1 cut(s) 256
BsiSI CCGG 2 cut(s) 107, 1337
BslFI GGGAC 4 cut(s) 727, 898, 1556, 1731
BslI CCNNNNNNNGG 7 cut(s) 16, 338, 353, 570, 914, 1376, 1786
BsmAI GTCTC 2 cut(s) 115, 1530
BsmBI CGTCTC 1 cut(s) 115
BsmFI GGGAC 4 cut(s) 727, 898, 1556, 1731
BsnI GGCC 4 cut(s) 414, 613, 918, 1303
Bsp1286I GDGCHC 4 cut(s) 256, 1135, 1506, 1763
Bsp13I TCCGGA 1 cut(s) 106
Bsp143I GATC 7 cut(s) 103, 136, 156, 223, 505, 998, 1315
BspACI CCGC 6 cut(s) 354, 599, 766, 919, 921, 1421
BspANI GGCC 4 cut(s) 414, 613, 918, 1303
BspCNI CTCAG 6 cut(s) 325, 892, 1021, 1336, 1441, 1540
BspDI ATCGAT 1 cut(s) 990
BspEI TCCGGA 1 cut(s) 106
BspFNI CGCG 1 cut(s) 921
BspHI TCATGA 2 cut(s) 1261, 1510
BspLI GGNNCC 1 cut(s) 1134
BspMAI CTGCAG 2 cut(s) 196, 421
BspPI GGATC 4 cut(s) 98, 144, 164, 1006
BspQI GCTCTTC 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 553
BsrFI RCCGGY 1 cut(s) 1336
BsrI ACTGG 5 cut(s) 347, 870, 1506, 1612, 1768
BssAI RCCGGY 1 cut(s) 1336
BssECI CCNNGG 4 cut(s) 10, 605, 919, 1747
BssMI GATC 7 cut(s) 103, 136, 156, 223, 505, 998, 1315
BssNI GRCGYC 1 cut(s) 1340
BssSI CACGAG 1 cut(s) 306
BssT1I CCWWGG 3 cut(s) 10, 605, 1747
Bst2BI CACGAG 1 cut(s) 306
Bst4CI ACNGT 2 cut(s) 953, 1666
Bst6I CTCTTC 2 cut(s) 42, 105
BstACI GRCGYC 1 cut(s) 1340
BstAPI GCANNNNNTGC 1 cut(s) 1808
BstC8I GCNNGC 3 cut(s) 344, 933, 1403
BstDEI CTNAG 7 cut(s) 333, 839, 900, 1029, 1344, 1449, 1527
BstDSI CCRYGG 1 cut(s) 919
BstEII GGTNACC 2 cut(s) 5, 648
BstENI CCTNNNNNAGG 1 cut(s) 14
BstF5I GGATG 7 cut(s) 132, 495, 642, 1007, 1045, 1443, 1795
BstFNI CGCG 1 cut(s) 921
BstKTI GATC 7 cut(s) 106, 139, 159, 226, 508, 1001, 1318
BstMAI GTCTC 2 cut(s) 115, 1530
BstMBI GATC 7 cut(s) 103, 136, 156, 223, 505, 998, 1315
BstMWI GCNNNNNNNGC 5 cut(s) 188, 1062, 1309, 1337, 1808
BstNSI RCATGY 7 cut(s) 742, 773, 935, 1196, 1405, 1409, 1502
BstPI GGTNACC 2 cut(s) 5, 648
BstSFI CTRYAG 3 cut(s) 192, 417, 1665
BstSLI GKGCMC 2 cut(s) 1506, 1763
BstUI CGCG 1 cut(s) 921
BstV1I GCAGC 4 cut(s) 178, 431, 1410, 1531
BstX2I RGATCY 3 cut(s) 505, 998, 1315
BstYI RGATCY 3 cut(s) 505, 998, 1315
Bsu15I ATCGAT 1 cut(s) 990
BsuI GTATCC 1 cut(s) 588
BsuRI GGCC 4 cut(s) 414, 613, 918, 1303
BsuTUI ATCGAT 1 cut(s) 990
BtgI CCRYGG 1 cut(s) 919
BtrI CACGTC 1 cut(s) 911
BtsCI GGATG 7 cut(s) 132, 495, 642, 1007, 1045, 1443, 1795
BtsIMutI CAGTG 5 cut(s) 94, 219, 958, 1230, 1761
Cac8I GCNNGC 3 cut(s) 344, 933, 1403
CaiI CAGNNNCTG 1 cut(s) 1001
CciI TCATGA 2 cut(s) 1261, 1510
Cfr10I RCCGGY 1 cut(s) 1336
Cfr13I GGNCC 3 cut(s) 23, 413, 1556
Cfr42I CCGCGG 1 cut(s) 922
ClaI ATCGAT 1 cut(s) 990
CseI GACGC 2 cut(s) 1071, 1329
Csp6I GTAC 3 cut(s) 972, 1071, 1367
CviQI GTAC 3 cut(s) 972, 1071, 1367
DdeI CTNAG 7 cut(s) 333, 839, 900, 1029, 1344, 1449, 1527
DpnI GATC 7 cut(s) 105, 138, 158, 225, 507, 1000, 1317
DpnII GATC 7 cut(s) 103, 136, 156, 223, 505, 998, 1315
DraIII CACNNNGTG 1 cut(s) 914
DriI GACNNNNNGTC 1 cut(s) 1699
EaeI YGGCCR 3 cut(s) 611, 916, 1301
Eam1104I CTCTTC 2 cut(s) 42, 105
Eam1105I GACNNNNNGTC 1 cut(s) 1699
EarI CTCTTC 2 cut(s) 42, 105
EciI GGCGGA 1 cut(s) 588
Eco130I CCWWGG 3 cut(s) 10, 605, 1747
Eco24I GRGCYC 1 cut(s) 1135
Eco47I GGWCC 2 cut(s) 23, 1556
Eco91I GGTNACC 2 cut(s) 5, 648
EcoNI CCTNNNNNAGG 1 cut(s) 14
EcoO65I GGTNACC 2 cut(s) 5, 648
EcoRI GAATTC 1 cut(s) 264
EcoT14I CCWWGG 3 cut(s) 10, 605, 1747
EcoT22I ATGCAT 2 cut(s) 1407, 1458
EcoT38I GRGCYC 1 cut(s) 1135
ErhI CCWWGG 3 cut(s) 10, 605, 1747
Esp3I CGTCTC 1 cut(s) 115
FalI AAGNNNNNCTT 2 cut(s) 343, 375
FaqI GGGAC 4 cut(s) 727, 898, 1556, 1731
FbaI TGATCA 1 cut(s) 223
Fnu4HI GCNGC 7 cut(s) 192, 420, 767, 919, 1424, 1520, 1812
FokI GGATG 7 cut(s) 139, 482, 649, 994, 1052, 1450, 1802
FriOI GRGCYC 1 cut(s) 1135
Fsp4HI GCNGC 7 cut(s) 192, 420, 767, 919, 1424, 1520, 1812
FspBI CTAG 2 cut(s) 942, 1053
GluI GCNGC 7 cut(s) 192, 420, 767, 919, 1424, 1520, 1812
GsuI CTGGAG 2 cut(s) 137, 1629
HaeIII GGCC 4 cut(s) 414, 613, 918, 1303
HapII CCGG 2 cut(s) 107, 1337
HgaI GACGC 2 cut(s) 1071, 1329
Hin1I GRCGYC 1 cut(s) 1340
HincII GTYRAC 1 cut(s) 720
HindII GTYRAC 1 cut(s) 720
HinfI GANTC 3 cut(s) 141, 445, 664
HpaI GTTAAC 1 cut(s) 720
HpaII CCGG 2 cut(s) 107, 1337
HphI GGTGA 3 cut(s) 17, 935, 1118
Hpy166II GTNNAC 1 cut(s) 720
Hpy188I TCNGA 9 cut(s) 40, 364, 374, 663, 1153, 1345, 1530, 1647, 1739
Hpy188III TCNNGA 8 cut(s) 107, 249, 332, 845, 1262, 1319, 1373, 1511
Hpy8I GTNNAC 1 cut(s) 720
Hpy99I CGWCG 3 cut(s) 915, 1065, 1393
HpyAV CCTTC 4 cut(s) 127, 228, 331, 1059
HpyCH4III ACNGT 2 cut(s) 953, 1666
HpyCH4IV ACGT 1 cut(s) 910
HpyF10VI GCNNNNNNNGC 5 cut(s) 188, 1062, 1309, 1337, 1808
HpyF3I CTNAG 7 cut(s) 333, 839, 900, 1029, 1344, 1449, 1527
HpySE526I ACGT 1 cut(s) 910
Hsp92I GRCGYC 1 cut(s) 1340
Kpn2I TCCGGA 1 cut(s) 106
Ksp22I TGATCA 1 cut(s) 223
KspAI GTTAAC 1 cut(s) 720
KspI CCGCGG 1 cut(s) 922
Kzo9I GATC 7 cut(s) 103, 136, 156, 223, 505, 998, 1315
LguI GCTCTTC 1 cut(s) 105
LmnI GCTCC 2 cut(s) 982, 1138
Lsp1109I GCAGC 4 cut(s) 178, 431, 1410, 1531
MaeI CTAG 2 cut(s) 942, 1053
MaeII ACGT 1 cut(s) 910
MaeIII GTNAC 6 cut(s) 5, 648, 947, 1175, 1288, 1381
MalI GATC 7 cut(s) 105, 138, 158, 225, 507, 1000, 1317
MboI GATC 7 cut(s) 103, 136, 156, 223, 505, 998, 1315
MboII GAAGA 7 cut(s) 59, 122, 250, 531, 638, 709, 860
MfeI CAATTG 1 cut(s) 1689
MflI RGATCY 3 cut(s) 505, 998, 1315
MhlI GDGCHC 4 cut(s) 256, 1135, 1506, 1763
MlyI GAGTC 1 cut(s) 673
MmeI TCCRAC 1 cut(s) 1762
Mph1103I ATGCAT 2 cut(s) 1407, 1458
MroI TCCGGA 1 cut(s) 106
MseI TTAA 5 cut(s) 692, 719, 1205, 1350, 1725
MslI CAYNNNNRTG 3 cut(s) 1233, 1406, 1410
MspA1I CMGCKG 4 cut(s) 725, 921, 1423, 1522
MspI CCGG 2 cut(s) 107, 1337
MunI CAATTG 1 cut(s) 1689
MvnI CGCG 1 cut(s) 921
MwoI GCNNNNNNNGC 5 cut(s) 188, 1062, 1309, 1337, 1808
NdeII GATC 7 cut(s) 103, 136, 156, 223, 505, 998, 1315
NlaIV GGNNCC 1 cut(s) 1134
NmuCI GTSAC 3 cut(s) 5, 1175, 1288
NsiI ATGCAT 2 cut(s) 1407, 1458
NspI RCATGY 7 cut(s) 742, 773, 935, 1196, 1405, 1409, 1502
PaeI GCATGC 2 cut(s) 935, 1405
PagI TCATGA 2 cut(s) 1261, 1510
PciI ACATGT 2 cut(s) 738, 1498
PciSI GCTCTTC 1 cut(s) 105
PfeI GAWTC 2 cut(s) 141, 445
PflMI CCANNNNNTGG 1 cut(s) 914
PkrI GCNGC 7 cut(s) 193, 421, 768, 920, 1425, 1521, 1813
PleI GAGTC 1 cut(s) 672
PpsI GAGTC 1 cut(s) 672
PscI ACATGT 2 cut(s) 738, 1498
PsiI TTATAA 1 cut(s) 1482
PspEI GGTNACC 2 cut(s) 5, 648
PspN4I GGNNCC 1 cut(s) 1134
PspPI GGNCC 3 cut(s) 23, 413, 1556
PstI CTGCAG 2 cut(s) 196, 421
PstNI CAGNNNCTG 1 cut(s) 1001
PsuI RGATCY 3 cut(s) 505, 998, 1315
PvuII CAGCTG 2 cut(s) 725, 1522
RsaI GTAC 3 cut(s) 973, 1072, 1368
RsaNI GTAC 3 cut(s) 972, 1071, 1367
RseI CAYNNNNRTG 3 cut(s) 1233, 1406, 1410
SacII CCGCGG 1 cut(s) 922
SapI GCTCTTC 1 cut(s) 105
SaqAI TTAA 5 cut(s) 692, 719, 1205, 1350, 1725
SatI GCNGC 7 cut(s) 192, 420, 767, 919, 1424, 1520, 1812
Sau3AI GATC 7 cut(s) 103, 136, 156, 223, 505, 998, 1315
Sau96I GGNCC 3 cut(s) 23, 413, 1556
ScaI AGTACT 1 cut(s) 1072
SchI GAGTC 1 cut(s) 673
SduI GDGCHC 4 cut(s) 256, 1135, 1506, 1763
SfcI CTRYAG 3 cut(s) 192, 417, 1665
Sfr303I CCGCGG 1 cut(s) 922
SgrBI CCGCGG 1 cut(s) 922
SinI GGWCC 2 cut(s) 23, 1556
SmiMI CAYNNNNRTG 3 cut(s) 1233, 1406, 1410
SmlI CTYRAG 1 cut(s) 55
SmoI CTYRAG 1 cut(s) 55
SphI GCATGC 2 cut(s) 935, 1405
SsiI CCGC 6 cut(s) 354, 599, 766, 919, 921, 1421
SspMI CTAG 2 cut(s) 942, 1053
StyI CCWWGG 3 cut(s) 10, 605, 1747
TaaI ACNGT 2 cut(s) 953, 1666
TaiI ACGT 1 cut(s) 913
TaqI TCGA 6 cut(s) 139, 232, 844, 862, 990, 1060
TatI WGTACW 1 cut(s) 1070
TauI GCSGC 2 cut(s) 769, 921
TfiI GAWTC 2 cut(s) 141, 445
Tru1I TTAA 5 cut(s) 692, 719, 1205, 1350, 1725
Tru9I TTAA 5 cut(s) 692, 719, 1205, 1350, 1725
TscAI CASTG 5 cut(s) 94, 226, 958, 1237, 1768
TseFI GTSAC 3 cut(s) 5, 1175, 1288
TseI GCWGC 5 cut(s) 191, 419, 1423, 1519, 1811
Tsp45I GTSAC 3 cut(s) 5, 1175, 1288
TspGWI ACGGA 2 cut(s) 139, 764
TspRI CASTG 5 cut(s) 94, 226, 958, 1237, 1768
Van91I CCANNNNNTGG 1 cut(s) 914
VpaK11BI GGWCC 2 cut(s) 23, 1556
XagI CCTNNNNNAGG 1 cut(s) 14
XapI RAATTY 1 cut(s) 264
XceI RCATGY 7 cut(s) 742, 773, 935, 1196, 1405, 1409, 1502
XspI CTAG 2 cut(s) 942, 1053
ZrmI AGTACT 1 cut(s) 1072
Zsp2I ATGCAT 2 cut(s) 1407, 1458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.