Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr11

Orthogroup ID

OG0008737

Physical Location & Seq

Genomic Coordinates Reverse (-)

1534665 .. 1537079

Gene Structure 2415 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom11g01800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1824 bp

ATGGGTGACGTTGGAGAGATTGGACCTGATATTAGTTCGGATATAGAAGAAGACTTGAGGTGCGATAATATTGCGGAGAAAGATGTCAGTGACGAAGAGATTGAAGCGGAAGATTTGGAGAGGCGAATGTGGAAGGATCGAATTAAGCTCAAAAGGCTTAAAGAAAGACAGAAACTTGAAGCTCAACAAGCTGCTGAAAAGCAGAAGCCAAAGCAGACCACTGATCAGGCTAGGAGAAAGAAAATGTCAAGAGCACAAGATGGGATTCTTAAGTATATGTTGAAGCTGATGGAAGTGTGCAAAGCTCGTGGTTTTGTGTATGGTATCATTCCTGAGAAGGGCAAGCCAGTGAGCGGTGCTTCAGATAACATCAGGGCTTGGTGGAAAGAAAAGGTGAAGTTCGATAAGAATGGTCCTGCAGCCATAGCCAAGCATGAAGCGGAGTGTCTTGCCATGAGTGATGCAGATAATAATCGAAATGGGAATTCTCAAAGCATCCTCCAAGATCTACAAGATGCAACTCTTGGTTCTCTATTGTCTTCTTTGATGCAACACTGTGACCCCCCTCAAAGGAAGTATCCATTAGAAAAGGGAGCTCCACCACCTTGGTGGCCGACAGGAAATGAAGATTGGTGGGTGAAACTAGGGTTAGTCCATGGTCAGATTCCTCCTTATAAGAAGCCACACGACCTAAAGAAGATGTGGAAAGTTGGAGTGTTAACAGCTGTGATAAAGCATATGTCACCTGATATTGCAAAGATAAGGAGGCATGTTCGCCAGTCAAAATGCTTACAGGATAAGATGACAGCAAAGGAAAGTGCAATTTGGTTAGGGGTTTTAAGCCGAGAAGAATCCCTCATTCGGCAGCCTAGTAGTGATAATGGGACATCTGGCATTACTGAGATGCCACAAAGTGGCCATGGCGAAAAGCAAGGTGCTGCTAGCAGTAACAGTGACTATGATGTTGATGGTACTGATGATGGGGTAGGCTCTGTTTCTTCATCCAAAGATGATAGGAGGAATCAGGTGATGGATGTGGATCCATCAAGCAATCTACACAATAATGCTCGTAATAATGTCCAAGATAAAGAGCAAGGTGAAAAGAAACCGAGGAGAAAAAGGGCACGTGTCAGAGCAAGCCCTGTTGAACGACGTCCTGCACCATCTCACAATGAGCATCTACATGTTCCACCAAGAGGTGCATTGCCTGATATAAACCACACTGATGTACAGATGATTGGACTTCAGGTTCATGAAAATCAACAGGAAAATGGTGCCATTACAACTTTACGGCCTCTGGAGAATGATCTTGATGTCCAAGAACAATTACCAGCGCCAGAGTTTAACTACTACCCTGGTGTACCTTCTGGCAATGTAATTACGACACAGGGCATGCATGTTGGTGGAACACCCTTGCTTTATCATGGGGTGCGAGATGCTGACATGCATCACGAAGATACATTTAACCAGCATCATGGAGATACATTTAACCTCTATAATCCATCAACACAATATCCCCCAAGTCATGACCGGCAGCCGCCTCAGATTGTAATGAATGAACCGCAGATTATACCAGCAGATGGAGTCCATGTACCAGTACATAGAAATGGAAGTGAAATTGCTGGAGGAGATTTTCCAAATTTTGTTCAGGACACATTTCAGAGTGAGCAAGACAGAACTGTCAATGCTAACTTTGGGTCTCCAATTGATAGCCTGTCGTTGGATTATGGATTGTTCAAAAGCCCATTCAACTTTGGAATTGACGGCACCGGTTCATTAGATGACCTTGAACTCGAAGAAATGATGGAGTACTTTGCGGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0035556 GO:0042221 GO:0042762 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071369 GO:0071495 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

EIN3

Protein Analysis

608

Amino Acids

68.0

Weight (kDa)

5.74

Isoelectric Point (pI)

56.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EIN3_DNA-bd	PF04873	37 - 284	1.5e-127	Ethylene insensitive 3, DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008737)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G73730 AT1G73730
fragaria_vesca	FvH4_3g43580 FvH4_3g43580
malus_domestica	MD03G1020800.v1.1 MD11G1022400.v1.1
prunus_persica	Prupe.6G018200_v2.0.a1 Prupe.6G018200_v2.0.a1
pyrus_communis	pycom03g01780 pycom11g01800
rosa_chinensis	RchiOBHm_Chr5g0078151
rosa_laevigata	RLG00000036758
rosa_multiflora	Rmu_sc0018349.1_g000004 Rmu_sc0018349.1_g000007
rosa_roxburghii	Rroxscaffold_1G00003980
rosa_rugosa	Rorug05G0459100 Rorug05G0459200 Rorug05G0459300
rosa_samantha	Rh5AG513600 Rh5BG536600 Rh5CG560000 Rh5DG548200
rosa_wichuraiana	Rw5G047660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	675
AasI	GACNNNNNNGTC	1 cut(s)	1679
AatII	GACGTC	1 cut(s)	1156
AccB1I	GGYRCC	2 cut(s)	1274, 1766
AccB7I	CCANNNNNTGG	2 cut(s)	914, 1580
AccBSI	CCGCTC	1 cut(s)	354
AciI	CCGC	7 cut(s)	74, 107, 354, 440, 1538, 1562, 1817
AclWI	GGATC	3 cut(s)	144, 1034, 1047
AcoI	YGGCCR	2 cut(s)	611, 916
AcsI	RAATTY	2 cut(s)	484, 1639
AcuI	CTGAAG	2 cut(s)	345, 1229
AcvI	CACGTG	1 cut(s)	1127
AcyI	GRCGYC	1 cut(s)	1153
AdeI	CACNNNGTG	1 cut(s)	914
AfaI	GTAC	6 cut(s)	973, 1230, 1362, 1593, 1599, 1811
AfiI	CCNNNNNNNGG	8 cut(s)	338, 353, 570, 861, 914, 1196, 1580, 1720
AflII	CTTAAG	1 cut(s)	269
AflIII	ACRYGT	2 cut(s)	1126, 1183
AgeI	ACCGGT	1 cut(s)	1769
AgsI	TTSAA	7 cut(s)	104, 179, 283, 1148, 1738, 1750, 1790
AjnI	CCWGG	1 cut(s)	1354
AjuI	GAANNNNNNNTTGG	2 cut(s)	613, 645
AleI	CACNNNNGTG	1 cut(s)	607
AloI	GAACNNNNNNTCC	2 cut(s)	1233, 1265
AluBI	AGCT	7 cut(s)	148, 182, 191, 286, 305, 596, 725
AluI	AGCT	7 cut(s)	148, 182, 191, 286, 305, 596, 725
Alw21I	GWGCWC	2 cut(s)	256, 598
Alw26I	GTCTC	1 cut(s)	1704
AlwI	GGATC	3 cut(s)	144, 1034, 1047
AoxI	GGCC	3 cut(s)	611, 916, 1292
ApeKI	GCWGC	5 cut(s)	191, 419, 865, 938, 1534
ApoI	RAATTY	2 cut(s)	484, 1639
AsiGI	ACCGGT	1 cut(s)	1769
AspLEI	GCGC	1 cut(s)	1336
AspS9I	GGNCC	2 cut(s)	23, 413
AsuHPI	GGTGA	6 cut(s)	17, 406, 649, 735, 1039, 1109
AsuNHI	GCTAGC	1 cut(s)	941
AvaII	GGWCC	2 cut(s)	23, 413
BaeGI	GKGCMC	1 cut(s)	1126
BalI	TGGCCA	1 cut(s)	918
BamHI	GGATCC	1 cut(s)	1039
BanI	GGYRCC	2 cut(s)	1274, 1766
BanII	GRGCYC	1 cut(s)	598
BauI	CACGAG	1 cut(s)	306
BbrPI	CACGTG	1 cut(s)	1127
BbsI	GAAGAC	2 cut(s)	57, 531
Bbv12I	GWGCWC	2 cut(s)	256, 598
BbvI	GCAGC	5 cut(s)	178, 431, 877, 925, 1546
BceAI	ACGGC	2 cut(s)	1307, 1780
BcgI	CGANNNNNNTGC	2 cut(s)	53, 87
BciT130I	CCWGG	1 cut(s)	1356
BciVI	GTATCC	1 cut(s)	588
BclI	TGATCA	1 cut(s)	223
BcoDI	GTCTC	1 cut(s)	1704
BfaI	CTAG	4 cut(s)	231, 644, 870, 942
BfmI	CTRYAG	1 cut(s)	417
BfoI	RGCGCY	1 cut(s)	1337
BfrI	CTTAAG	1 cut(s)	269
BfuI	GTATCC	1 cut(s)	588
BglII	AGATCT	1 cut(s)	505
BisI	GCNGC	7 cut(s)	192, 420, 866, 939, 1535, 1538, 1818
BlsI	GCNGC	7 cut(s)	193, 421, 867, 940, 1536, 1539, 1819
BmcAI	AGTACT	1 cut(s)	1811
Bme1390I	CCNGG	1 cut(s)	1356
Bme18I	GGWCC	2 cut(s)	23, 413
BmgT120I	GGNCC	2 cut(s)	23, 413
BmiI	GGNNCC	3 cut(s)	1041, 1276, 1768
BmrFI	CCNGG	1 cut(s)	1356
BmsI	GCATC	9 cut(s)	451, 504, 505, 537, 894, 1186, 1426, 1456, 1480
BmtI	GCTAGC	1 cut(s)	945
BpiI	GAAGAC	2 cut(s)	57, 531
BpmI	CTGGAG	2 cut(s)	1319, 1644
BpuEI	CTTGAG	1 cut(s)	76
BsaAI	YACGTR	1 cut(s)	1127
BsaBI	GATNNNNATC	2 cut(s)	471, 1038
BsaHI	GRCGYC	1 cut(s)	1153
BsaI	GGTCTC	1 cut(s)	1704
BsaJI	CCNNGG	5 cut(s)	605, 655, 919, 1109, 1354
BsaWI	WCCGGW	1 cut(s)	1769
BsaXI	ACNNNNNCTCC	2 cut(s)	1575, 1605
Bsc4I	CCNNNNNNNGG	8 cut(s)	338, 353, 570, 861, 914, 1196, 1580, 1720
Bse118I	RCCGGY	2 cut(s)	1530, 1769
Bse1I	ACTGG	3 cut(s)	347, 778, 1595
Bse3DI	GCAATG	2 cut(s)	1202, 1378
Bse8I	GATNNNNATC	2 cut(s)	471, 1038
BseBI	CCWGG	1 cut(s)	1356
BseDI	CCNNGG	5 cut(s)	605, 655, 919, 1109, 1354
BseGI	GGATG	3 cut(s)	495, 1001, 1039
BseJI	GATNNNNATC	2 cut(s)	471, 1038
BseLI	CCNNNNNNNGG	8 cut(s)	338, 353, 570, 861, 914, 1196, 1580, 1720
BseMI	GCAATG	2 cut(s)	1202, 1378
BseMII	CTCAG	3 cut(s)	324, 891, 1556
BseNI	ACTGG	3 cut(s)	347, 778, 1595
BseRI	GAGGAG	2 cut(s)	1126, 1641
BseSI	GKGCMC	1 cut(s)	1126
BseXI	GCAGC	5 cut(s)	178, 431, 877, 925, 1546
BsgI	GTGCAG	1 cut(s)	1143
BshFI	GGCC	3 cut(s)	613, 918, 1294
BshNI	GGYRCC	2 cut(s)	1274, 1766
BshTI	ACCGGT	1 cut(s)	1769
BsiHKAI	GWGCWC	2 cut(s)	256, 598
BsiSI	CCGG	2 cut(s)	1531, 1770
BslFI	GGGAC	1 cut(s)	898
BslI	CCNNNNNNNGG	8 cut(s)	338, 353, 570, 861, 914, 1196, 1580, 1720
BsmAI	GTCTC	1 cut(s)	1704
BsmFI	GGGAC	1 cut(s)	898
BsnI	GGCC	3 cut(s)	613, 918, 1294
Bso31I	GGTCTC	1 cut(s)	1704
Bsp1286I	GDGCHC	3 cut(s)	256, 598, 1126
Bsp1407I	TGTACA	1 cut(s)	1228
Bsp143I	GATC	5 cut(s)	136, 223, 505, 1039, 1306
Bsp19I	CCATGG	2 cut(s)	655, 919
BspACI	CCGC	7 cut(s)	74, 107, 354, 440, 1538, 1562, 1817
BspANI	GGCC	3 cut(s)	613, 918, 1294
BspCNI	CTCAG	3 cut(s)	325, 892, 1555
BspHI	TCATGA	2 cut(s)	1252, 1525
BspLI	GGNNCC	3 cut(s)	1041, 1276, 1768
BspMAI	CTGCAG	1 cut(s)	421
BspOI	GCTAGC	1 cut(s)	945
BspPI	GGATC	3 cut(s)	144, 1034, 1047
BspT107I	GGYRCC	2 cut(s)	1274, 1766
BspTI	CTTAAG	1 cut(s)	269
BspTNI	GGTCTC	1 cut(s)	1704
BsrBI	CCGCTC	1 cut(s)	354
BsrDI	GCAATG	2 cut(s)	1202, 1378
BsrFI	RCCGGY	2 cut(s)	1530, 1769
BsrGI	TGTACA	1 cut(s)	1228
BsrI	ACTGG	3 cut(s)	347, 778, 1595
BssAI	RCCGGY	2 cut(s)	1530, 1769
BssECI	CCNNGG	5 cut(s)	605, 655, 919, 1109, 1354
BssMI	GATC	5 cut(s)	136, 223, 505, 1039, 1306
BssNI	GRCGYC	1 cut(s)	1153
BssSI	CACGAG	1 cut(s)	306
BssT1I	CCWWGG	3 cut(s)	605, 655, 919
Bst2BI	CACGAG	1 cut(s)	306
Bst2UI	CCWGG	1 cut(s)	1356
Bst4CI	ACNGT	3 cut(s)	557, 953, 1681
Bst6I	CTCTTC	1 cut(s)	90
BstACI	GRCGYC	1 cut(s)	1153
BstAFI	CTTAAG	1 cut(s)	269
BstAUI	TGTACA	1 cut(s)	1228
BstBAI	YACGTR	1 cut(s)	1127
BstC8I	GCNNGC	4 cut(s)	344, 943, 1138, 1394
BstDEI	CTNAG	3 cut(s)	333, 900, 1542
BstDSI	CCRYGG	2 cut(s)	655, 919
BstF5I	GGATG	3 cut(s)	495, 1001, 1039
BstH2I	RGCGCY	1 cut(s)	1337
BstHHI	GCGC	1 cut(s)	1336
BstKTI	GATC	5 cut(s)	139, 226, 508, 1042, 1309
BstMAI	GTCTC	1 cut(s)	1704
BstMBI	GATC	5 cut(s)	136, 223, 505, 1039, 1306
BstMWI	GCNNNNNNNGC	3 cut(s)	154, 188, 425
BstNI	CCWGG	1 cut(s)	1356
BstNSI	RCATGY	5 cut(s)	773, 1187, 1396, 1400, 1447
BstSCI	CCNGG	1 cut(s)	1354
BstSFI	CTRYAG	1 cut(s)	417
BstSLI	GKGCMC	1 cut(s)	1126
BstV1I	GCAGC	5 cut(s)	178, 431, 877, 925, 1546
BstV2I	GAAGAC	2 cut(s)	57, 531
BstX2I	RGATCY	2 cut(s)	505, 1039
BstXI	CCANNNNNNTGG	3 cut(s)	606, 609, 1475
BstYI	RGATCY	2 cut(s)	505, 1039
BsuI	GTATCC	1 cut(s)	588
BsuRI	GGCC	3 cut(s)	613, 918, 1294
BtgI	CCRYGG	2 cut(s)	655, 919
BtsCI	GGATG	3 cut(s)	495, 1001, 1039
BtsIMutI	CAGTG	6 cut(s)	94, 219, 354, 553, 958, 1221
Cac8I	GCNNGC	4 cut(s)	344, 943, 1138, 1394
CciI	TCATGA	2 cut(s)	1252, 1525
CfoI	GCGC	1 cut(s)	1336
Cfr10I	RCCGGY	2 cut(s)	1530, 1769
Cfr13I	GGNCC	2 cut(s)	23, 413
Csp6I	GTAC	6 cut(s)	972, 1229, 1361, 1592, 1598, 1810
CspAI	ACCGGT	1 cut(s)	1769
CspCI	CAANNNNNGTGG	2 cut(s)	289, 324
CviQI	GTAC	6 cut(s)	972, 1229, 1361, 1592, 1598, 1810
DdeI	CTNAG	3 cut(s)	333, 900, 1542
DpnI	GATC	5 cut(s)	138, 225, 507, 1041, 1308
DpnII	GATC	5 cut(s)	136, 223, 505, 1039, 1306
DraIII	CACNNNGTG	1 cut(s)	914
DrdI	GACNNNNNNGTC	1 cut(s)	1679
DseDI	GACNNNNNNGTC	1 cut(s)	1679
EaeI	YGGCCR	2 cut(s)	611, 916
Eam1104I	CTCTTC	1 cut(s)	90
EarI	CTCTTC	1 cut(s)	90
Ecl136II	GAGCTC	1 cut(s)	596
Eco130I	CCWWGG	3 cut(s)	605, 655, 919
Eco24I	GRGCYC	1 cut(s)	598
Eco31I	GGTCTC	1 cut(s)	1704
Eco47I	GGWCC	2 cut(s)	23, 413
Eco53kI	GAGCTC	1 cut(s)	596
Eco57I	CTGAAG	2 cut(s)	345, 1229
Eco72I	CACGTG	1 cut(s)	1127
EcoICRI	GAGCTC	1 cut(s)	596
EcoRI	GAATTC	1 cut(s)	484
EcoRII	CCWGG	1 cut(s)	1354
EcoT14I	CCWWGG	3 cut(s)	605, 655, 919
EcoT22I	ATGCAT	2 cut(s)	1398, 1449
EcoT38I	GRGCYC	1 cut(s)	598
ErhI	CCWWGG	3 cut(s)	605, 655, 919
FaqI	GGGAC	1 cut(s)	898
FauNDI	CATATG	1 cut(s)	738
FbaI	TGATCA	1 cut(s)	223
Fnu4HI	GCNGC	7 cut(s)	192, 420, 866, 939, 1535, 1538, 1818
FokI	GGATG	3 cut(s)	482, 988, 1046
FriOI	GRGCYC	1 cut(s)	598
Fsp4HI	GCNGC	7 cut(s)	192, 420, 866, 939, 1535, 1538, 1818
FspBI	CTAG	4 cut(s)	231, 644, 870, 942
GlaI	GCGC	1 cut(s)	1335
GluI	GCNGC	7 cut(s)	192, 420, 866, 939, 1535, 1538, 1818
GsuI	CTGGAG	2 cut(s)	1319, 1644
HaeII	RGCGCY	1 cut(s)	1337
HaeIII	GGCC	3 cut(s)	613, 918, 1294
HapII	CCGG	2 cut(s)	1531, 1770
HhaI	GCGC	1 cut(s)	1336
Hin1I	GRCGYC	1 cut(s)	1153
Hin6I	GCGC	1 cut(s)	1334
HinP1I	GCGC	1 cut(s)	1334
HincII	GTYRAC	1 cut(s)	720
HindII	GTYRAC	1 cut(s)	720
HinfI	GANTC	5 cut(s)	265, 664, 851, 1021, 1584
HpaI	GTTAAC	1 cut(s)	720
HpaII	CCGG	2 cut(s)	1531, 1770
HphI	GGTGA	6 cut(s)	17, 406, 649, 735, 1039, 1109
Hpy166II	GTNNAC	2 cut(s)	720, 1361
Hpy188I	TCNGA	6 cut(s)	40, 364, 663, 1133, 1545, 1662
Hpy188III	TCNNGA	8 cut(s)	249, 332, 1253, 1298, 1310, 1451, 1526, 1649
Hpy8I	GTNNAC	2 cut(s)	720, 1361
Hpy99I	CGWCG	1 cut(s)	1155
HpyAV	CCTTC	3 cut(s)	127, 331, 1374
HpyCH4III	ACNGT	3 cut(s)	557, 953, 1681
HpyCH4IV	ACGT	3 cut(s)	9, 1126, 1153
HpyF10VI	GCNNNNNNNGC	3 cut(s)	154, 188, 425
HpyF3I	CTNAG	3 cut(s)	333, 900, 1542
HpySE526I	ACGT	3 cut(s)	9, 1126, 1153
Hsp92I	GRCGYC	1 cut(s)	1153
HspAI	GCGC	1 cut(s)	1334
Ksp22I	TGATCA	1 cut(s)	223
KspAI	GTTAAC	1 cut(s)	720
Kzo9I	GATC	5 cut(s)	136, 223, 505, 1039, 1306
LmnI	GCTCC	2 cut(s)	593, 601
Lsp1109I	GCAGC	5 cut(s)	178, 431, 877, 925, 1546
LweI	GCATC	9 cut(s)	451, 504, 505, 537, 894, 1186, 1426, 1456, 1480
MaeI	CTAG	4 cut(s)	231, 644, 870, 942
MaeII	ACGT	3 cut(s)	9, 1126, 1153
MaeIII	GTNAC	6 cut(s)	5, 89, 557, 741, 947, 953
MalI	GATC	5 cut(s)	138, 225, 507, 1041, 1308
MbiI	CCGCTC	1 cut(s)	354
MboI	GATC	5 cut(s)	136, 223, 505, 1039, 1306
MfeI	CAATTG	1 cut(s)	1704
MflI	RGATCY	2 cut(s)	505, 1039
MhlI	GDGCHC	3 cut(s)	256, 598, 1126
MlsI	TGGCCA	1 cut(s)	918
MluCI	AATT	9 cut(s)	141, 484, 822, 1325, 1377, 1617, 1639, 1704, 1758
MluNI	TGGCCA	1 cut(s)	918
MlyI	GAGTC	1 cut(s)	1593
MmeI	TCCRAC	2 cut(s)	691, 1701
Mox20I	TGGCCA	1 cut(s)	918
Mph1103I	ATGCAT	2 cut(s)	1398, 1449
MscI	TGGCCA	1 cut(s)	918
MseI	TTAA	8 cut(s)	144, 159, 270, 719, 839, 1344, 1464, 1488
MslI	CAYNNNNRTG	6 cut(s)	607, 1062, 1182, 1224, 1401, 1605
Msp20I	TGGCCA	1 cut(s)	918
MspA1I	CMGCKG	1 cut(s)	725
MspCI	CTTAAG	1 cut(s)	269
MspI	CCGG	2 cut(s)	1531, 1770
MspR9I	CCNGG	1 cut(s)	1356
MunI	CAATTG	1 cut(s)	1704
MvaI	CCWGG	1 cut(s)	1356
MwoI	GCNNNNNNNGC	3 cut(s)	154, 188, 425
NcoI	CCATGG	2 cut(s)	655, 919
NdeI	CATATG	1 cut(s)	738
NdeII	GATC	5 cut(s)	136, 223, 505, 1039, 1306
NheI	GCTAGC	1 cut(s)	941
NlaIV	GGNNCC	3 cut(s)	1041, 1276, 1768
NmeAIII	GCCGAG	1 cut(s)	869
NmuCI	GTSAC	5 cut(s)	5, 89, 557, 741, 953
NsiI	ATGCAT	2 cut(s)	1398, 1449
NspI	RCATGY	5 cut(s)	773, 1187, 1396, 1400, 1447
OliI	CACNNNNGTG	1 cut(s)	607
PaeI	GCATGC	1 cut(s)	1396
PagI	TCATGA	2 cut(s)	1252, 1525
PciI	ACATGT	1 cut(s)	1183
PfeI	GAWTC	4 cut(s)	265, 664, 851, 1021
PflMI	CCANNNNNTGG	2 cut(s)	914, 1580
PinAI	ACCGGT	1 cut(s)	1769
PkrI	GCNGC	7 cut(s)	193, 421, 867, 940, 1536, 1539, 1819
PleI	GAGTC	1 cut(s)	1592
PmaCI	CACGTG	1 cut(s)	1127
PmlI	CACGTG	1 cut(s)	1127
PpsI	GAGTC	1 cut(s)	1592
Ppu21I	YACGTR	1 cut(s)	1127
PscI	ACATGT	1 cut(s)	1183
PsiI	TTATAA	1 cut(s)	675
Psp124BI	GAGCTC	1 cut(s)	598
Psp6I	CCWGG	1 cut(s)	1354
PspCI	CACGTG	1 cut(s)	1127
PspGI	CCWGG	1 cut(s)	1354
PspN4I	GGNNCC	3 cut(s)	1041, 1276, 1768
PspPI	GGNCC	2 cut(s)	23, 413
PstI	CTGCAG	1 cut(s)	421
PsuI	RGATCY	2 cut(s)	505, 1039
PvuII	CAGCTG	1 cut(s)	725
RsaI	GTAC	6 cut(s)	973, 1230, 1362, 1593, 1599, 1811
RsaNI	GTAC	6 cut(s)	972, 1229, 1361, 1592, 1598, 1810
RseI	CAYNNNNRTG	6 cut(s)	607, 1062, 1182, 1224, 1401, 1605
SacI	GAGCTC	1 cut(s)	598
SaqAI	TTAA	8 cut(s)	144, 159, 270, 719, 839, 1344, 1464, 1488
SatI	GCNGC	7 cut(s)	192, 420, 866, 939, 1535, 1538, 1818
Sau3AI	GATC	5 cut(s)	136, 223, 505, 1039, 1306
Sau96I	GGNCC	2 cut(s)	23, 413
ScaI	AGTACT	1 cut(s)	1811
SchI	GAGTC	1 cut(s)	1593
ScrFI	CCNGG	1 cut(s)	1356
SduI	GDGCHC	3 cut(s)	256, 598, 1126
SfaNI	GCATC	9 cut(s)	451, 504, 505, 537, 894, 1186, 1426, 1456, 1480
SfcI	CTRYAG	1 cut(s)	417
SinI	GGWCC	2 cut(s)	23, 413
SmiMI	CAYNNNNRTG	6 cut(s)	607, 1062, 1182, 1224, 1401, 1605
SmlI	CTYRAG	2 cut(s)	55, 269
SmoI	CTYRAG	2 cut(s)	55, 269
SphI	GCATGC	1 cut(s)	1396
Sse9I	AATT	9 cut(s)	141, 484, 822, 1325, 1377, 1617, 1639, 1704, 1758
SsiI	CCGC	7 cut(s)	74, 107, 354, 440, 1538, 1562, 1817
SspI	AATATT	1 cut(s)	70
SspMI	CTAG	4 cut(s)	231, 644, 870, 942
SstI	GAGCTC	1 cut(s)	598
StyD4I	CCNGG	1 cut(s)	1354
StyI	CCWWGG	3 cut(s)	605, 655, 919
TaaI	ACNGT	3 cut(s)	557, 953, 1681
TaiI	ACGT	3 cut(s)	12, 1129, 1156
TaqI	TCGA	4 cut(s)	139, 402, 475, 1794
TasI	AATT	9 cut(s)	141, 484, 822, 1325, 1377, 1617, 1639, 1704, 1758
TatI	WGTACW	3 cut(s)	1228, 1597, 1809
TauI	GCSGC	2 cut(s)	1540, 1820
TfiI	GAWTC	4 cut(s)	265, 664, 851, 1021
Tru1I	TTAA	8 cut(s)	144, 159, 270, 719, 839, 1344, 1464, 1488
Tru9I	TTAA	8 cut(s)	144, 159, 270, 719, 839, 1344, 1464, 1488
TscAI	CASTG	6 cut(s)	94, 226, 354, 560, 958, 1228
TseFI	GTSAC	5 cut(s)	5, 89, 557, 741, 953
TseI	GCWGC	5 cut(s)	191, 419, 865, 938, 1534
Tsp45I	GTSAC	5 cut(s)	5, 89, 557, 741, 953
TspDTI	ATGAA	8 cut(s)	450, 639, 990, 1241, 1269, 1568, 1572, 1764
TspRI	CASTG	6 cut(s)	94, 226, 354, 560, 958, 1228
Van91I	CCANNNNNTGG	2 cut(s)	914, 1580
Vha464I	CTTAAG	1 cut(s)	269
VpaK11BI	GGWCC	2 cut(s)	23, 413
XapI	RAATTY	2 cut(s)	484, 1639
XceI	RCATGY	5 cut(s)	773, 1187, 1396, 1400, 1447
XcmI	CCANNNNNNNNNTGG	1 cut(s)	606
XspI	CTAG	4 cut(s)	231, 644, 870, 942
ZraI	GACGTC	1 cut(s)	1154
ZrmI	AGTACT	1 cut(s)	1811
Zsp2I	ATGCAT	2 cut(s)	1398, 1449

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.