Rorug05G0459300

ETHYLENE INSENSITIVE 3-like 3

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
Physical Location & Seq
Forward (+)
63238405 .. 63240663
2259 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0459300.1

Sequence Viewer

Length: 2025 bp
ATGGGCAAGACTACCCTTGCAAAGTTAGTGTTTGAGAGAACTTCCCATCATTTTGAAGTTAGCAAGTTTCTCCTTAACGTTGGTGAGGTTTTTGCAAAACATGGTACTTTAGTTGATCTACAAAAGCAACTTCTTTCACCAATCTTAAAGGAAAATATTTGGGATGAGCAAAGAGGAACCTTTCTCATTAGGAAATGCTTGTTTAATAAAAAAGTTCTTCTCGTTGTTGATAATGTTGATCACTATAACCAACTAGAAATGTTGGTTGGATATAAATCATGGTTTGGTGAGGGAAGCAGAATCATTATTACAACTAGAGATGAACGTTTGCTCATCGAGCATGATATAGAATTATCATTTAAGGCACAAGGACTAAATAGCAACAAAGCTCTGGAGCTCTTTTGTCAGAATGCGTTCAAAAAAGATCAACCTGATGAAGGGTTTTCAAAACTGTCCAAGTGTTTTGTAGATTATGCCAAAGGACTTCCATTAGCTCTTAAAATTTTGGGACGTTCTTTGTATAAGAGAGATCTAGATGCATGGAATAGTGCATTTAATAAACTCAAGAAGATTCCTATTCCAACAATATATGATTCGCTCAAACTAAGTTTTGATGCACTGGATGATATAGAGAAGGGTATTTTTCTTGATGTTGCATGTTTCTACAATGGGATGGAGAAAAGACGAGTAGTTGAAATACTAGAAAGCTGTGGCATTTGTAGTCATATTGGGATAGATGTTCTCATTCAGAAATCTCTGTTATTTGTTGGGAGAGACAACAATCTAGAGATGCATGATTTGATACAAGAAATGGCATGGGAAATTGTTCGTCAAGAGTCCTGTGAAGAACCTGGTCTACGTAGTCGTTTGTGCCATCGTAAAGATATCTTTCATGTATTCATGAGAAATATGGGAACAGAAGCAATCAGAGGCATCCGCTTAAGCTTATTAGAATTACAAGTGGCAGACTTAAACTGGAATTGTGAAGGCTTCTCTAAGATGTTGAAACTAATGTTTCTCGAATTTGATAATTTAATTATCTTTTGTGCGCCCAAATTCCTCCCCAATTCTTTAAGAATTCTCAAGTGGAGTTGGTATCCTTCCAAATTTCTTCCAGCAAGTTACCGACCAAATTTACTAGTTGAACTTAAGATGGAAAACAGTAAACTTGTTCGGCTTTGGGATGGAAAACAGGACTTGCCTAACCTGAAATATTTGGATCTTAGCCGCTCCAAAAACTTGACCAAGACTCCAGATTTCACAGGTATTCCGAATCTTGAAAGTCTAAGCTTTTTATATTGTACACGTTTGGTTGAGATCCACCCATCCATTGCAGTTAACAAAAAGCATAAAGTGTTGAACCTTTTTGGATGCAAAAGTATTAAGAGTTTCTCAAGTGAGATAGATATGGATTCTCTCGAGTATTTCAACCTTAATGAGTGCTCAAAACTAAAGATTCCAGAATTTTCTAGAGGGATGAAAAAATTGTCATCTCTTGACCTAGGTGGGACTGCTATTCAGAAAATACCTTCATCAGTTGAACATTTGGTTAGCCTTACTTATCTGGATCTAAGTTATTGCAGAAGTCTCATATGCCTTCCGAGTGCCATTTTTAAACTGAAGTCTCTTAAAAGCCTCAATATGGATGGATGCTTTAAACTTCAGAAGCTCCTAGAAAACACGGGGGAGATGGAGTCTTTAGAGAAGCAGCTTTGGGTCAGTCAAACTGCTAAGAGAGAGCCGCCACCCTTCCTTCATATGAAATATCTCAGAAGACTAAGTTTTGGTGCGTCGCCTTTTATAAGGATGCTTGGCGCGGGCTTTTGTGAGCCTTTGCGCATGGCGTTGCCTTCTCAAAGGACTTTACGCTCTTTGGAGTCGTTAACTCTAATTGATGCTAATCTTGGTCAAGGAGCTATTCCGGATGATATTGGCTACTGCTTGTCATCTATACGTATCTTGGTTCTTAGTGGAAATGATTTTGTTAGTCTTCCTACAAGTATTAATTGCCTTCTTGTTCATTAA

Protein Analysis

674

Amino Acids

77.1

Weight (kDa)

8.77

Isoelectric Point (pI)

41.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 1 - 142 2e-12 NB-ARC domain
WHD_ROQ1 PF23282 211 - 280 1.9e-19 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 471 - 556 1.8e-07 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 493 - 671 1.5e-11 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1802
Acc16I TGCGCA 1 cut(s) 1838
AccBSI CCGCTC 1 cut(s) 1230
AccI GTMKAC 1 cut(s) 856
AccII CGCG 1 cut(s) 1817
AccIII TCCGGA 1 cut(s) 1921
AciI CCGC 4 cut(s) 937, 1228, 1742, 1817
AclI AACGTT 2 cut(s) 78, 325
AclWI GGATC 3 cut(s) 1227, 1312, 1575
AcsI RAATTY 7 cut(s) 501, 1022, 1055, 1077, 1106, 1132, 1463
AcuI CTGAAG 2 cut(s) 1640, 1646
AfaI GTAC 2 cut(s) 106, 1303
AfiI CCNNNNNNNGG 2 cut(s) 437, 1642
AflII CTTAAG 2 cut(s) 940, 1148
AflIII ACRYGT 1 cut(s) 1304
AhlI ACTAGT 1 cut(s) 1138
AjnI CCWGG 1 cut(s) 850
AjuI GAANNNNNNNTTGG 2 cut(s) 249, 281
AluBI AGCT 9 cut(s) 389, 397, 494, 708, 945, 1290, 1669, 1711, 1916
AluI AGCT 9 cut(s) 389, 397, 494, 708, 945, 1290, 1669, 1711, 1916
Alw21I GWGCWC 2 cut(s) 399, 1445
Alw26I GTCTC 3 cut(s) 768, 1592, 1629
AlwI GGATC 3 cut(s) 1227, 1312, 1575
Ama87I CYCGRG 1 cut(s) 1418
Aor13HI TCCGGA 1 cut(s) 1921
ApeKI GCWGC 1 cut(s) 1708
ApoI RAATTY 7 cut(s) 501, 1022, 1055, 1077, 1106, 1132, 1463
AseI ATTAAT 1 cut(s) 2004
Asp700I GAANNNNTTC 2 cut(s) 413, 825
AspA2I CCTAGG 1 cut(s) 1501
AspLEI GCGC 3 cut(s) 1051, 1817, 1839
AsuHPI GGTGA 3 cut(s) 95, 129, 299
AvaI CYCGRG 1 cut(s) 1418
AvrII CCTAGG 1 cut(s) 1501
BaeI ACNNNNGTAYC 2 cut(s) 96, 129
BanII GRGCYC 1 cut(s) 399
BbsI GAAGAC 2 cut(s) 1780, 1982
Bbv12I GWGCWC 2 cut(s) 399, 1445
BbvI GCAGC 1 cut(s) 1720
BccI CCATC 8 cut(s) 54, 667, 882, 1147, 1178, 1333, 1640, 1684
BciT130I CCWGG 1 cut(s) 852
BciVI GTATCC 1 cut(s) 1107
BclI TGATCA 1 cut(s) 238
BcoDI GTCTC 3 cut(s) 768, 1592, 1629
BcuI ACTAGT 1 cut(s) 1138
BfaI CTAG 9 cut(s) 254, 315, 533, 701, 785, 1139, 1470, 1502, 1673
BfrI CTTAAG 2 cut(s) 940, 1148
BfuI GTATCC 1 cut(s) 1107
BglII AGATCT 1 cut(s) 529
BisI GCNGC 3 cut(s) 1228, 1709, 1742
BlnI CCTAGG 1 cut(s) 1501
BlsI GCNGC 3 cut(s) 1229, 1710, 1743
Bme1390I CCNGG 1 cut(s) 852
BmeT110I CYCGRG 1 cut(s) 1418
BmiI GGNNCC 1 cut(s) 178
BmrFI CCNGG 1 cut(s) 852
BmsI GCATC 8 cut(s) 526, 604, 780, 942, 1361, 1640, 1797, 1885
BpiI GAAGAC 2 cut(s) 1780, 1982
BpmI CTGGAG 2 cut(s) 413, 1236
BpuEI CTTGAG 3 cut(s) 548, 1067, 1378
BsaAI YACGTR 2 cut(s) 860, 1955
BsaBI GATNNNNATC 2 cut(s) 274, 1899
BsaJI CCNNGG 1 cut(s) 1501
BsaWI WCCGGW 1 cut(s) 1921
BsaXI ACNNNNNCTCC 4 cut(s) 1234, 1264, 1679, 1709
Bsc4I CCNNNNNNNGG 2 cut(s) 437, 1642
Bse1I ACTGG 2 cut(s) 624, 980
Bse3DI GCAATG 1 cut(s) 1329
Bse8I GATNNNNATC 2 cut(s) 274, 1899
BseAI TCCGGA 1 cut(s) 1921
BseBI CCWGG 1 cut(s) 852
BseDI CCNNGG 1 cut(s) 1501
BseJI GATNNNNATC 2 cut(s) 274, 1899
BseLI CCNNNNNNNGG 2 cut(s) 437, 1642
BseMI GCAATG 1 cut(s) 1329
BseMII CTCAG 1 cut(s) 1783
BseNI ACTGG 2 cut(s) 624, 980
BseXI GCAGC 1 cut(s) 1720
Bsh1236I CGCG 1 cut(s) 1817
BsiHKAI GWGCWC 2 cut(s) 399, 1445
BsiHKCI CYCGRG 1 cut(s) 1418
BsiSI CCGG 1 cut(s) 1922
BslFI GGGAC 2 cut(s) 522, 1522
BslI CCNNNNNNNGG 2 cut(s) 437, 1642
BsmAI GTCTC 3 cut(s) 768, 1592, 1629
BsmFI GGGAC 2 cut(s) 522, 1522
BsmI GAATGC 1 cut(s) 415
BsoBI CYCGRG 1 cut(s) 1418
Bsp1286I GDGCHC 2 cut(s) 399, 1445
Bsp13I TCCGGA 1 cut(s) 1921
Bsp1407I TGTACA 1 cut(s) 1301
Bsp143I GATC 7 cut(s) 115, 238, 424, 529, 1219, 1317, 1567
BspACI CCGC 4 cut(s) 937, 1228, 1742, 1817
BspCNI CTCAG 1 cut(s) 1782
BspEI TCCGGA 1 cut(s) 1921
BspFNI CGCG 1 cut(s) 1817
BspHI TCATGA 1 cut(s) 900
BspLI GGNNCC 1 cut(s) 178
BspPI GGATC 3 cut(s) 1227, 1312, 1575
BspTI CTTAAG 2 cut(s) 940, 1148
BsrBI CCGCTC 1 cut(s) 1230
BsrDI GCAATG 1 cut(s) 1329
BsrGI TGTACA 1 cut(s) 1301
BsrI ACTGG 2 cut(s) 624, 980
BssECI CCNNGG 1 cut(s) 1501
BssMI GATC 7 cut(s) 115, 238, 424, 529, 1219, 1317, 1567
BssT1I CCWWGG 1 cut(s) 1501
Bst2UI CCWGG 1 cut(s) 852
Bst4CI ACNGT 2 cut(s) 453, 1163
BstAFI CTTAAG 2 cut(s) 940, 1148
BstAUI TGTACA 1 cut(s) 1301
BstBAI YACGTR 2 cut(s) 860, 1955
BstC8I GCNNGC 1 cut(s) 1819
BstDEI CTNAG 9 cut(s) 605, 996, 1223, 1286, 1571, 1731, 1769, 1778, 1967
BstENI CCTNNNNNAGG 1 cut(s) 435
BstFNI CGCG 1 cut(s) 1817
BstHHI GCGC 3 cut(s) 1051, 1817, 1839
BstKTI GATC 7 cut(s) 118, 241, 427, 532, 1222, 1320, 1570
BstMAI GTCTC 3 cut(s) 768, 1592, 1629
BstMBI GATC 7 cut(s) 115, 238, 424, 529, 1219, 1317, 1567
BstMWI GCNNNNNNNGC 1 cut(s) 337
BstNI CCWGG 1 cut(s) 852
BstNSI RCATGY 1 cut(s) 660
BstSCI CCNGG 1 cut(s) 850
BstSNI TACGTA 2 cut(s) 860, 1955
BstUI CGCG 1 cut(s) 1817
BstV1I GCAGC 1 cut(s) 1720
BstV2I GAAGAC 2 cut(s) 1780, 1982
BstX2I RGATCY 4 cut(s) 529, 1219, 1317, 1567
BstYI RGATCY 4 cut(s) 529, 1219, 1317, 1567
BsuI GTATCC 1 cut(s) 1107
BtsIMutI CAGTG 1 cut(s) 617
Cac8I GCNNGC 1 cut(s) 1819
CciI TCATGA 1 cut(s) 900
CfoI GCGC 3 cut(s) 1051, 1817, 1839
CseI GACGC 1 cut(s) 1779
CsiI ACCWGGT 1 cut(s) 850
Csp6I GTAC 2 cut(s) 105, 1302
CviQI GTAC 2 cut(s) 105, 1302
DdeI CTNAG 9 cut(s) 605, 996, 1223, 1286, 1571, 1731, 1769, 1778, 1967
DpnI GATC 7 cut(s) 117, 240, 426, 531, 1221, 1319, 1569
DpnII GATC 7 cut(s) 115, 238, 424, 529, 1219, 1317, 1567
DraI TTTAAA 2 cut(s) 1615, 1657
Ecl136II GAGCTC 1 cut(s) 397
Eco105I TACGTA 2 cut(s) 860, 1955
Eco130I CCWWGG 1 cut(s) 1501
Eco24I GRGCYC 1 cut(s) 399
Eco32I GATATC 1 cut(s) 886
Eco53kI GAGCTC 1 cut(s) 397
Eco57I CTGAAG 2 cut(s) 1640, 1646
Eco88I CYCGRG 1 cut(s) 1418
EcoICRI GAGCTC 1 cut(s) 397
EcoNI CCTNNNNNAGG 1 cut(s) 435
EcoRI GAATTC 1 cut(s) 1077
EcoRII CCWGG 1 cut(s) 850
EcoRV GATATC 1 cut(s) 886
EcoT14I CCWWGG 1 cut(s) 1501
EcoT22I ATGCAT 2 cut(s) 541, 795
EcoT38I GRGCYC 1 cut(s) 399
ErhI CCWWGG 1 cut(s) 1501
FaqI GGGAC 2 cut(s) 522, 1522
FauI CCCGC 1 cut(s) 1810
FauNDI CATATG 2 cut(s) 1592, 1758
FbaI TGATCA 1 cut(s) 238
FblI GTMKAC 1 cut(s) 856
Fnu4HI GCNGC 3 cut(s) 1228, 1709, 1742
FriOI GRGCYC 1 cut(s) 399
Fsp4HI GCNGC 3 cut(s) 1228, 1709, 1742
FspBI CTAG 9 cut(s) 254, 315, 533, 701, 785, 1139, 1470, 1502, 1673
FspI TGCGCA 1 cut(s) 1838
GlaI GCGC 3 cut(s) 1050, 1816, 1838
GluI GCNGC 3 cut(s) 1228, 1709, 1742
GsuI CTGGAG 2 cut(s) 413, 1236
HapII CCGG 1 cut(s) 1922
HgaI GACGC 1 cut(s) 1779
HhaI GCGC 3 cut(s) 1051, 1817, 1839
Hin6I GCGC 3 cut(s) 1049, 1815, 1837
HinP1I GCGC 3 cut(s) 1049, 1815, 1837
HincII GTYRAC 2 cut(s) 1339, 1884
HindII GTYRAC 2 cut(s) 1339, 1884
HindIII AAGCTT 2 cut(s) 943, 1288
HpaI GTTAAC 2 cut(s) 1339, 1884
HpaII CCGG 1 cut(s) 1922
HphI GGTGA 3 cut(s) 95, 129, 299
Hpy166II GTNNAC 5 cut(s) 857, 1166, 1304, 1339, 1884
Hpy188I TCNGA 8 cut(s) 408, 750, 929, 1272, 1521, 1602, 1665, 1772
Hpy8I GTNNAC 5 cut(s) 857, 1166, 1304, 1339, 1884
Hpy99I CGWCG 1 cut(s) 1795
HpyCH4III ACNGT 2 cut(s) 453, 1163
HpyCH4IV ACGT 6 cut(s) 78, 325, 511, 859, 1306, 1954
HpyF10VI GCNNNNNNNGC 1 cut(s) 337
HpyF3I CTNAG 9 cut(s) 605, 996, 1223, 1286, 1571, 1731, 1769, 1778, 1967
HpySE526I ACGT 6 cut(s) 78, 325, 511, 859, 1306, 1954
HspAI GCGC 3 cut(s) 1049, 1815, 1837
Kpn2I TCCGGA 1 cut(s) 1921
Ksp22I TGATCA 1 cut(s) 238
KspAI GTTAAC 2 cut(s) 1339, 1884
Kzo9I GATC 7 cut(s) 115, 238, 424, 529, 1219, 1317, 1567
LmnI GCTCC 4 cut(s) 394, 1235, 1674, 1913
Lsp1109I GCAGC 1 cut(s) 1720
LweI GCATC 8 cut(s) 526, 604, 780, 942, 1361, 1640, 1797, 1885
MabI ACCWGGT 1 cut(s) 850
MaeI CTAG 9 cut(s) 254, 315, 533, 701, 785, 1139, 1470, 1502, 1673
MaeII ACGT 6 cut(s) 78, 325, 511, 859, 1306, 1954
MaeIII GTNAC 1 cut(s) 1121
MalI GATC 7 cut(s) 117, 240, 426, 531, 1221, 1319, 1569
MbiI CCGCTC 1 cut(s) 1230
MboI GATC 7 cut(s) 115, 238, 424, 529, 1219, 1317, 1567
MboII GAAGA 6 cut(s) 209, 580, 857, 1103, 1785, 1982
MflI RGATCY 4 cut(s) 529, 1219, 1317, 1567
MhlI GDGCHC 2 cut(s) 399, 1445
MlyI GAGTC 4 cut(s) 845, 1243, 1703, 1886
MmeI TCCRAC 2 cut(s) 247, 605
MnlI CCTC 7 cut(s) 79, 167, 283, 923, 1070, 1466, 1646
Mph1103I ATGCAT 2 cut(s) 541, 795
MroI TCCGGA 1 cut(s) 1921
MroXI GAANNNNTTC 2 cut(s) 413, 825
MspCI CTTAAG 2 cut(s) 940, 1148
MspI CCGG 1 cut(s) 1922
MspR9I CCNGG 1 cut(s) 852
Mva1269I GAATGC 1 cut(s) 415
MvaI CCWGG 1 cut(s) 852
MvnI CGCG 1 cut(s) 1817
MwoI GCNNNNNNNGC 1 cut(s) 337
NdeI CATATG 2 cut(s) 1592, 1758
NdeII GATC 7 cut(s) 115, 238, 424, 529, 1219, 1317, 1567
NlaIV GGNNCC 1 cut(s) 178
NsbI TGCGCA 1 cut(s) 1838
NsiI ATGCAT 2 cut(s) 541, 795
NspI RCATGY 1 cut(s) 660
PaeR7I CTCGAG 1 cut(s) 1418
PagI TCATGA 1 cut(s) 900
PctI GAATGC 1 cut(s) 415
PdmI GAANNNNTTC 2 cut(s) 413, 825
PfeI GAWTC 6 cut(s) 300, 571, 593, 1273, 1412, 1456
PkrI GCNGC 3 cut(s) 1229, 1710, 1743
PleI GAGTC 4 cut(s) 844, 1243, 1702, 1885
PpsI GAGTC 4 cut(s) 844, 1243, 1702, 1885
Ppu21I YACGTR 2 cut(s) 860, 1955
PshBI ATTAAT 1 cut(s) 2004
PsiI TTATAA 1 cut(s) 1802
Psp124BI GAGCTC 1 cut(s) 399
Psp1406I AACGTT 2 cut(s) 78, 325
Psp6I CCWGG 1 cut(s) 850
PspGI CCWGG 1 cut(s) 850
PspN4I GGNNCC 1 cut(s) 178
PsrI GAACNNNNNNTAC 2 cut(s) 840, 872
PsuI RGATCY 4 cut(s) 529, 1219, 1317, 1567
RsaI GTAC 2 cut(s) 106, 1303
RsaNI GTAC 2 cut(s) 105, 1302
SacI GAGCTC 1 cut(s) 399
SatI GCNGC 3 cut(s) 1228, 1709, 1742
Sau3AI GATC 7 cut(s) 115, 238, 424, 529, 1219, 1317, 1567
SchI GAGTC 4 cut(s) 845, 1243, 1703, 1886
ScrFI CCNGG 1 cut(s) 852
SduI GDGCHC 2 cut(s) 399, 1445
SexAI ACCWGGT 1 cut(s) 850
SfaNI GCATC 8 cut(s) 526, 604, 780, 942, 1361, 1640, 1797, 1885
Sfr274I CTCGAG 1 cut(s) 1418
SlaI CTCGAG 1 cut(s) 1418
SmlI CTYRAG 6 cut(s) 563, 940, 1082, 1148, 1393, 1418
SmoI CTYRAG 6 cut(s) 563, 940, 1082, 1148, 1393, 1418
SnaBI TACGTA 2 cut(s) 860, 1955
SpeI ACTAGT 1 cut(s) 1138
SsiI CCGC 4 cut(s) 937, 1228, 1742, 1817
SspI AATATT 2 cut(s) 157, 1214
SspMI CTAG 9 cut(s) 254, 315, 533, 701, 785, 1139, 1470, 1502, 1673
SstI GAGCTC 1 cut(s) 399
StyD4I CCNGG 1 cut(s) 850
StyI CCWWGG 1 cut(s) 1501
TaaI ACNGT 2 cut(s) 453, 1163
TaiI ACGT 6 cut(s) 81, 328, 514, 862, 1309, 1957
TaqI TCGA 3 cut(s) 336, 1020, 1419
TatI WGTACW 1 cut(s) 1301
TauI GCSGC 2 cut(s) 1230, 1744
TfiI GAWTC 6 cut(s) 300, 571, 593, 1273, 1412, 1456
TscAI CASTG 1 cut(s) 624
TseI GCWGC 1 cut(s) 1708
TspDTI ATGAA 9 cut(s) 336, 450, 881, 889, 1493, 1521, 1745, 1775, 2009
TspRI CASTG 1 cut(s) 624
Vha464I CTTAAG 2 cut(s) 940, 1148
VspI ATTAAT 1 cut(s) 2004
XagI CCTNNNNNAGG 1 cut(s) 435
XapI RAATTY 7 cut(s) 501, 1022, 1055, 1077, 1106, 1132, 1463
XbaI TCTAGA 3 cut(s) 532, 784, 1469
XceI RCATGY 1 cut(s) 660
XhoI CTCGAG 1 cut(s) 1418
XmaJI CCTAGG 1 cut(s) 1501
XmiI GTMKAC 1 cut(s) 856
XmnI GAANNNNTTC 2 cut(s) 413, 825
XspI CTAG 9 cut(s) 254, 315, 533, 701, 785, 1139, 1470, 1502, 1673
Zsp2I ATGCAT 2 cut(s) 541, 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.