Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0008737

Physical Location & Seq

Genomic Coordinates Forward (+)

84198302 .. 84200690

Gene Structure 2389 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000036758

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1818 bp

ATGGGGGACCTTGGAGAGGTTGGACCTGAAATTAGTTCGGATATAGAAGAAGACTTGAGGTGTGATAATTTAGTGGAGAAAGATGTCAGTGACGAGGAGATTGATCCGGAAGAGCTGGAGAGACGGATGTGGAAGGATCGAATCAAGCTCAAAAGGATCAAAGAAAAACAGAAAATTGAGGCCCAACAAGCCGCGGAAAGGCAGAAGCCCAAACAGACCACTGATCAGGCTCGAAGGAAGAAAATGTCAAGAGCACAAGATGGAATTCTAAAGTATATGTTGAAGCTGATGGAAGTGTGTCAAGCTCGTGGATTTGTGTATGGTATCATTCCTGAGAAGGGCAAGCCAGTAAGCGGTGCTTCTGATAACATCAGAGCTTGGTGGAAAGAAAAAGTGAAGTTTGATAAGAATGGCCCTGCAGCCATAGACAAGTATGAAGCAGAGATTCTTGCCATGACTGATGCAGACAATAACCGAAATGGTAATTCCCAGACCATCCTCCAAGATCTACAAGATGCAACTCTTGGTTCTCTACTGTCTTCATTGATGCAACACTGCGACCCCCCTCAAAGGAAGTATCCATTAGAAAAGGCAGTTCCACCTCCTTGGTGGCCAACAGGAAATGAAGATTGGTGGATGAAATCAGGGTTACCCTGTGGTCAGAGTCCTCCTTATAAGAAGCCGCATGACTTAAAGAAGATGTGGAAAGTTGGGGTGTTAACAGCTGTGATAAAGCACATGTCCCCTGATATTGCAAAGATAAGGCGGCATGTCCGTCAGTCAAAATGCTTACAGGATAAGATGACAGCAAAGGAGAGTGCAATTTGGTTGGGGGTTCTAAGTCGAGAAGAAGCCCTCATTCGACAACCCAGTAGCGATAATGGGACATCTGGCATAACTGAGATGCCACGTCGTGGCCGTGGTGAAAAGCATGCTACTGTTAGTAGTAACAGTGATTATGATGTTGATGGTACTCATAAGGAGCTAATCGATGCAGGATCTGTTTCATCCAAAGATGACAGGAGGACTGAGCTGATGGATGTAGAGCCATCTAGCAAGCTACGCAGTAGTACTCCTACTAATCATGTCCAAGATAAAGAGAGAGGTGAAAAGCGAAGAAAGAGAAAAAGGGCTCCTGTACGACCAAGCTCTGATGATAAACTTCCTGCACCAAGTCACAATGAGCCTTTGCATGTTGAACCATTAAATGCTTTGCCTGATATAAACCACACTGATGCACAGATGATTGGATTTCCAATTCATGAAAATCAACAGGAAAATATTTCTGTCACAACTTTACGGCCACCAGAGCAAGATCTTGATGTCCAAGCATTACCGGCGTCCGAGTTTAACTACTATGCTGATGTACCACCTGACGGTGTAACTGCGACGACACAGGGCATGCATGTGGGTGGAACACCGCTGCTTTATCATGGGGTGCAAAGTGCTGAGATGCATCATGGAAATATGTATAACCTTTATAATCCATCAGCAGAATATGTGCCCGGTCATGATAGGCAGCAGTCTCAGATTGGCATGAATGAACTGCAAATTGGACCAGCAGATGTAGTCCCTTTACAAACTGTAAGAAATGGAAGTGAAATTACTGGAGGAGATATACCATTCTATGCAAAAGACCCATTTCAGAGTGAGCAAGACAGAACTGTCGATGCTAACTTTGGCTCTCCAATTGACAGCCTGTCATTAGATTATGGGGGACTATTTAACAGCCCATACCGACTTGACCTTGGAATTGAGGGCACTGGTTCATTAGATGACCTGAATGTGGATGAAATGATGGCATACTTTGCAGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0035556 GO:0042221 GO:0042762 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071369 GO:0071495 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

EIN3

Protein Analysis

606

Amino Acids

67.8

Weight (kDa)

5.57

Isoelectric Point (pI)

62.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EIN3_DNA-bd	PF04873	37 - 284	7.6e-127	Ethylene insensitive 3, DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008737)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G73730 AT1G73730
fragaria_vesca	FvH4_3g43580 FvH4_3g43580
malus_domestica	MD03G1020800.v1.1 MD11G1022400.v1.1
prunus_persica	Prupe.6G018200_v2.0.a1 Prupe.6G018200_v2.0.a1
pyrus_communis	pycom03g01780 pycom11g01800
rosa_chinensis	RchiOBHm_Chr5g0078151
rosa_laevigata	RLG00000036758
rosa_multiflora	Rmu_sc0018349.1_g000004 Rmu_sc0018349.1_g000007
rosa_roxburghii	Rroxscaffold_1G00003980
rosa_rugosa	Rorug05G0459100 Rorug05G0459200 Rorug05G0459300
rosa_samantha	Rh5AG513600 Rh5BG536600 Rh5CG560000 Rh5DG548200
rosa_wichuraiana	Rw5G047660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	675, 1482
AasI	GACNNNNNNGTC	1 cut(s)	1664
AccB7I	CCANNNNNTGG	1 cut(s)	914
AccII	CGCG	1 cut(s)	194
AccIII	TCCGGA	1 cut(s)	106
AciI	CCGC	6 cut(s)	192, 194, 354, 683, 766, 1421
AclWI	GGATC	4 cut(s)	98, 144, 164, 1006
AcoI	YGGCCR	3 cut(s)	611, 916, 1301
AcsI	RAATTY	1 cut(s)	264
AcyI	GRCGYC	1 cut(s)	1340
AdeI	CACNNNGTG	1 cut(s)	914
AfaI	GTAC	4 cut(s)	973, 1072, 1140, 1368
AfiI	CCNNNNNNNGG	8 cut(s)	16, 198, 338, 353, 570, 914, 1376, 1786
AflIII	ACRYGT	1 cut(s)	738
AgsI	TTSAA	2 cut(s)	283, 1199
AhdI	GACNNNNNGTC	1 cut(s)	1699
AjiI	CACGTC	1 cut(s)	911
AjuI	GAANNNNNNNTTGG	4 cut(s)	613, 645, 1536, 1568
Alw21I	GWGCWC	1 cut(s)	256
Alw26I	GTCTC	2 cut(s)	115, 1530
AlwI	GGATC	4 cut(s)	98, 144, 164, 1006
AlwNI	CAGNNNCTG	1 cut(s)	1001
Aor13HI	TCCGGA	1 cut(s)	106
AoxI	GGCC	5 cut(s)	180, 412, 611, 916, 1301
ApeKI	GCWGC	4 cut(s)	419, 1423, 1519, 1811
ApoI	RAATTY	1 cut(s)	264
AspS9I	GGNCC	5 cut(s)	7, 23, 181, 413, 1556
AsuC2I	CCSGG	1 cut(s)	1506
AsuHPI	GGTGA	2 cut(s)	935, 1118
AvaII	GGWCC	3 cut(s)	7, 23, 1556
BaeGI	GKGCMC	2 cut(s)	1506, 1763
BalI	TGGCCA	1 cut(s)	613
BanII	GRGCYC	1 cut(s)	1135
BarI	GAAGNNNNNNTAC	2 cut(s)	1588, 1620
BauI	CACGAG	1 cut(s)	306
BbsI	GAAGAC	2 cut(s)	57, 531
Bbv12I	GWGCWC	1 cut(s)	256
BbvI	GCAGC	3 cut(s)	431, 1410, 1531
BccI	CCATC	8 cut(s)	254, 283, 503, 962, 1030, 1057, 1495, 1792
BceAI	ACGGC	2 cut(s)	903, 1316
BciVI	GTATCC	1 cut(s)	588
BclI	TGATCA	1 cut(s)	223
BcnI	CCSGG	1 cut(s)	1506
BcoDI	GTCTC	2 cut(s)	115, 1530
BfaI	CTAG	1 cut(s)	1053
BfmI	CTRYAG	1 cut(s)	417
BfuI	GTATCC	1 cut(s)	588
BglII	AGATCT	2 cut(s)	505, 1315
BisI	GCNGC	7 cut(s)	192, 420, 683, 767, 1424, 1520, 1812
BlsI	GCNGC	7 cut(s)	193, 421, 684, 768, 1425, 1521, 1813
BmcAI	AGTACT	1 cut(s)	1072
Bme1390I	CCNGG	1 cut(s)	1506
Bme18I	GGWCC	3 cut(s)	7, 23, 1556
BmeRI	GACNNNNNGTC	1 cut(s)	1699
BmgBI	CACGTC	1 cut(s)	911
BmgT120I	GGNCC	5 cut(s)	7, 23, 181, 413, 1556
BmiI	GGNNCC	2 cut(s)	8, 1134
BmrFI	CCNGG	1 cut(s)	1506
BmrI	ACTGGG	1 cut(s)	864
BmsI	GCATC	9 cut(s)	451, 505, 537, 894, 982, 1225, 1443, 1465, 1660
BmuI	ACTGGG	1 cut(s)	864
BpiI	GAAGAC	2 cut(s)	57, 531
BpmI	CTGGAG	2 cut(s)	137, 1629
BpuEI	CTTGAG	1 cut(s)	76
BpuMI	CCSGG	1 cut(s)	1506
Bsa29I	ATCGAT	1 cut(s)	990
BsaHI	GRCGYC	1 cut(s)	1340
BsaJI	CCNNGG	5 cut(s)	10, 192, 605, 919, 1747
BsaWI	WCCGGW	1 cut(s)	106
Bsc4I	CCNNNNNNNGG	8 cut(s)	16, 198, 338, 353, 570, 914, 1376, 1786
Bse118I	RCCGGY	1 cut(s)	1336
Bse1I	ACTGG	4 cut(s)	347, 870, 1612, 1768
BseAI	TCCGGA	1 cut(s)	106
BseCI	ATCGAT	1 cut(s)	990
BseDI	CCNNGG	5 cut(s)	10, 192, 605, 919, 1747
BseGI	GGATG	6 cut(s)	132, 495, 642, 1007, 1045, 1795
BseLI	CCNNNNNNNGG	8 cut(s)	16, 198, 338, 353, 570, 914, 1376, 1786
BseMII	CTCAG	5 cut(s)	324, 891, 1020, 1440, 1541
BseNI	ACTGG	4 cut(s)	347, 870, 1612, 1768
BseRI	GAGGAG	2 cut(s)	110, 1626
BseSI	GKGCMC	2 cut(s)	1506, 1763
BseXI	GCAGC	3 cut(s)	431, 1410, 1531
BsgI	GTGCAG	1 cut(s)	1152
Bsh1236I	CGCG	1 cut(s)	194
BshFI	GGCC	5 cut(s)	182, 414, 613, 918, 1303
BshVI	ATCGAT	1 cut(s)	990
BsiHKAI	GWGCWC	1 cut(s)	256
BsiSI	CCGG	3 cut(s)	107, 1337, 1506
BslFI	GGGAC	5 cut(s)	20, 727, 898, 1556, 1731
BslI	CCNNNNNNNGG	8 cut(s)	16, 198, 338, 353, 570, 914, 1376, 1786
BsmAI	GTCTC	2 cut(s)	115, 1530
BsmBI	CGTCTC	1 cut(s)	115
BsmFI	GGGAC	5 cut(s)	20, 727, 898, 1556, 1731
BsnI	GGCC	5 cut(s)	182, 414, 613, 918, 1303
Bsp1286I	GDGCHC	4 cut(s)	256, 1135, 1506, 1763
Bsp13I	TCCGGA	1 cut(s)	106
Bsp143I	GATC	7 cut(s)	103, 136, 156, 223, 505, 998, 1315
BspACI	CCGC	6 cut(s)	192, 194, 354, 683, 766, 1421
BspANI	GGCC	5 cut(s)	182, 414, 613, 918, 1303
BspCNI	CTCAG	5 cut(s)	325, 892, 1021, 1441, 1540
BspDI	ATCGAT	1 cut(s)	990
BspEI	TCCGGA	1 cut(s)	106
BspFNI	CGCG	1 cut(s)	194
BspHI	TCATGA	2 cut(s)	1261, 1510
BspLI	GGNNCC	2 cut(s)	8, 1134
BspMAI	CTGCAG	1 cut(s)	421
BspPI	GGATC	4 cut(s)	98, 144, 164, 1006
BspQI	GCTCTTC	1 cut(s)	105
BsrFI	RCCGGY	1 cut(s)	1336
BsrI	ACTGG	4 cut(s)	347, 870, 1612, 1768
BssAI	RCCGGY	1 cut(s)	1336
BssECI	CCNNGG	5 cut(s)	10, 192, 605, 919, 1747
BssMI	GATC	7 cut(s)	103, 136, 156, 223, 505, 998, 1315
BssNI	GRCGYC	1 cut(s)	1340
BssSI	CACGAG	1 cut(s)	306
BssT1I	CCWWGG	3 cut(s)	10, 605, 1747
Bst2BI	CACGAG	1 cut(s)	306
Bst4CI	ACNGT	6 cut(s)	537, 940, 953, 1379, 1585, 1666
Bst6I	CTCTTC	1 cut(s)	105
BstACI	GRCGYC	1 cut(s)	1340
BstAPI	GCANNNNNTGC	1 cut(s)	1808
BstC8I	GCNNGC	4 cut(s)	344, 933, 1058, 1403
BstDEI	CTNAG	6 cut(s)	333, 839, 900, 1029, 1449, 1527
BstDSI	CCRYGG	2 cut(s)	192, 919
BstEII	GGTNACC	1 cut(s)	648
BstENI	CCTNNNNNAGG	1 cut(s)	14
BstF5I	GGATG	6 cut(s)	132, 495, 642, 1007, 1045, 1795
BstFNI	CGCG	1 cut(s)	194
BstKTI	GATC	7 cut(s)	106, 139, 159, 226, 508, 1001, 1318
BstMAI	GTCTC	2 cut(s)	115, 1530
BstMBI	GATC	7 cut(s)	103, 136, 156, 223, 505, 998, 1315
BstMWI	GCNNNNNNNGC	5 cut(s)	188, 1062, 1309, 1337, 1808
BstNSI	RCATGY	6 cut(s)	742, 773, 935, 1196, 1405, 1409
BstPI	GGTNACC	1 cut(s)	648
BstSCI	CCNGG	1 cut(s)	1504
BstSFI	CTRYAG	1 cut(s)	417
BstSLI	GKGCMC	2 cut(s)	1506, 1763
BstUI	CGCG	1 cut(s)	194
BstV1I	GCAGC	3 cut(s)	431, 1410, 1531
BstV2I	GAAGAC	2 cut(s)	57, 531
BstX2I	RGATCY	3 cut(s)	505, 998, 1315
BstXI	CCANNNNNNTGG	1 cut(s)	606
BstYI	RGATCY	3 cut(s)	505, 998, 1315
Bsu15I	ATCGAT	1 cut(s)	990
BsuI	GTATCC	1 cut(s)	588
BsuRI	GGCC	5 cut(s)	182, 414, 613, 918, 1303
BsuTUI	ATCGAT	1 cut(s)	990
BtgI	CCRYGG	2 cut(s)	192, 919
BtrI	CACGTC	1 cut(s)	911
BtsCI	GGATG	6 cut(s)	132, 495, 642, 1007, 1045, 1795
BtsI	GCAGTG	1 cut(s)	553
BtsIMutI	CAGTG	6 cut(s)	94, 219, 553, 958, 1230, 1761
Cac8I	GCNNGC	4 cut(s)	344, 933, 1058, 1403
CaiI	CAGNNNCTG	1 cut(s)	1001
CciI	TCATGA	2 cut(s)	1261, 1510
Cfr10I	RCCGGY	1 cut(s)	1336
Cfr13I	GGNCC	5 cut(s)	7, 23, 181, 413, 1556
Cfr42I	CCGCGG	1 cut(s)	195
ClaI	ATCGAT	1 cut(s)	990
CseI	GACGC	1 cut(s)	1329
Csp6I	GTAC	4 cut(s)	972, 1071, 1139, 1367
CviQI	GTAC	4 cut(s)	972, 1071, 1139, 1367
DdeI	CTNAG	6 cut(s)	333, 839, 900, 1029, 1449, 1527
DpnI	GATC	7 cut(s)	105, 138, 158, 225, 507, 1000, 1317
DpnII	GATC	7 cut(s)	103, 136, 156, 223, 505, 998, 1315
DraIII	CACNNNGTG	1 cut(s)	914
DrdI	GACNNNNNNGTC	1 cut(s)	1664
DriI	GACNNNNNGTC	1 cut(s)	1699
DseDI	GACNNNNNNGTC	1 cut(s)	1664
EaeI	YGGCCR	3 cut(s)	611, 916, 1301
Eam1104I	CTCTTC	1 cut(s)	105
Eam1105I	GACNNNNNGTC	1 cut(s)	1699
EarI	CTCTTC	1 cut(s)	105
Eco130I	CCWWGG	3 cut(s)	10, 605, 1747
Eco24I	GRGCYC	1 cut(s)	1135
Eco47I	GGWCC	3 cut(s)	7, 23, 1556
Eco91I	GGTNACC	1 cut(s)	648
EcoNI	CCTNNNNNAGG	1 cut(s)	14
EcoO109I	RGGNCCY	1 cut(s)	7
EcoO65I	GGTNACC	1 cut(s)	648
EcoRI	GAATTC	1 cut(s)	264
EcoT14I	CCWWGG	3 cut(s)	10, 605, 1747
EcoT22I	ATGCAT	2 cut(s)	1407, 1458
EcoT38I	GRGCYC	1 cut(s)	1135
ErhI	CCWWGG	3 cut(s)	10, 605, 1747
Esp3I	CGTCTC	1 cut(s)	115
FalI	AAGNNNNNCTT	2 cut(s)	343, 375
FaqI	GGGAC	5 cut(s)	20, 727, 898, 1556, 1731
FbaI	TGATCA	1 cut(s)	223
Fnu4HI	GCNGC	7 cut(s)	192, 420, 683, 767, 1424, 1520, 1812
FokI	GGATG	6 cut(s)	139, 482, 649, 994, 1052, 1802
FriOI	GRGCYC	1 cut(s)	1135
Fsp4HI	GCNGC	7 cut(s)	192, 420, 683, 767, 1424, 1520, 1812
FspBI	CTAG	1 cut(s)	1053
GluI	GCNGC	7 cut(s)	192, 420, 683, 767, 1424, 1520, 1812
GsuI	CTGGAG	2 cut(s)	137, 1629
HaeIII	GGCC	5 cut(s)	182, 414, 613, 918, 1303
HapII	CCGG	3 cut(s)	107, 1337, 1506
HgaI	GACGC	1 cut(s)	1329
Hin1I	GRCGYC	1 cut(s)	1340
HincII	GTYRAC	1 cut(s)	720
HindII	GTYRAC	1 cut(s)	720
HinfI	GANTC	3 cut(s)	141, 445, 664
HpaI	GTTAAC	1 cut(s)	720
HpaII	CCGG	3 cut(s)	107, 1337, 1506
HphI	GGTGA	2 cut(s)	935, 1118
Hpy166II	GTNNAC	1 cut(s)	720
Hpy188I	TCNGA	8 cut(s)	40, 364, 374, 663, 1153, 1345, 1530, 1647
Hpy188III	TCNNGA	7 cut(s)	107, 249, 332, 845, 1262, 1319, 1511
Hpy8I	GTNNAC	1 cut(s)	720
Hpy99I	CGWCG	2 cut(s)	915, 1393
HpyAV	CCTTC	3 cut(s)	127, 228, 331
HpyCH4III	ACNGT	6 cut(s)	537, 940, 953, 1379, 1585, 1666
HpyCH4IV	ACGT	1 cut(s)	910
HpyF10VI	GCNNNNNNNGC	5 cut(s)	188, 1062, 1309, 1337, 1808
HpyF3I	CTNAG	6 cut(s)	333, 839, 900, 1029, 1449, 1527
HpySE526I	ACGT	1 cut(s)	910
Hsp92I	GRCGYC	1 cut(s)	1340
Kpn2I	TCCGGA	1 cut(s)	106
Ksp22I	TGATCA	1 cut(s)	223
KspAI	GTTAAC	1 cut(s)	720
KspI	CCGCGG	1 cut(s)	195
Kzo9I	GATC	7 cut(s)	103, 136, 156, 223, 505, 998, 1315
LguI	GCTCTTC	1 cut(s)	105
LmnI	GCTCC	2 cut(s)	982, 1138
Lsp1109I	GCAGC	3 cut(s)	431, 1410, 1531
LweI	GCATC	9 cut(s)	451, 505, 537, 894, 982, 1225, 1443, 1465, 1660
MaeI	CTAG	1 cut(s)	1053
MaeII	ACGT	1 cut(s)	910
MaeIII	GTNAC	6 cut(s)	89, 648, 947, 1175, 1288, 1381
MalI	GATC	7 cut(s)	105, 138, 158, 225, 507, 1000, 1317
MboI	GATC	7 cut(s)	103, 136, 156, 223, 505, 998, 1315
MboII	GAAGA	9 cut(s)	59, 62, 122, 250, 531, 638, 709, 860, 1128
MfeI	CAATTG	1 cut(s)	1689
MflI	RGATCY	3 cut(s)	505, 998, 1315
MhlI	GDGCHC	4 cut(s)	256, 1135, 1506, 1763
MlsI	TGGCCA	1 cut(s)	613
MluNI	TGGCCA	1 cut(s)	613
MlyI	GAGTC	1 cut(s)	673
Mox20I	TGGCCA	1 cut(s)	613
Mph1103I	ATGCAT	2 cut(s)	1407, 1458
MroI	TCCGGA	1 cut(s)	106
MscI	TGGCCA	1 cut(s)	613
MseI	TTAA	5 cut(s)	692, 719, 1205, 1350, 1725
MslI	CAYNNNNRTG	3 cut(s)	1233, 1406, 1410
Msp20I	TGGCCA	1 cut(s)	613
MspA1I	CMGCKG	3 cut(s)	194, 725, 1423
MspI	CCGG	3 cut(s)	107, 1337, 1506
MspR9I	CCNGG	1 cut(s)	1506
MunI	CAATTG	1 cut(s)	1689
MvnI	CGCG	1 cut(s)	194
MwoI	GCNNNNNNNGC	5 cut(s)	188, 1062, 1309, 1337, 1808
NciI	CCSGG	1 cut(s)	1506
NdeII	GATC	7 cut(s)	103, 136, 156, 223, 505, 998, 1315
NlaIV	GGNNCC	2 cut(s)	8, 1134
NmuCI	GTSAC	3 cut(s)	89, 1175, 1288
NsiI	ATGCAT	2 cut(s)	1407, 1458
NspI	RCATGY	6 cut(s)	742, 773, 935, 1196, 1405, 1409
PaeI	GCATGC	2 cut(s)	935, 1405
PagI	TCATGA	2 cut(s)	1261, 1510
PciI	ACATGT	1 cut(s)	738
PciSI	GCTCTTC	1 cut(s)	105
PcsI	WCGNNNNNNNCGW	1 cut(s)	916
PfeI	GAWTC	2 cut(s)	141, 445
PflMI	CCANNNNNTGG	1 cut(s)	914
PkrI	GCNGC	7 cut(s)	193, 421, 684, 768, 1425, 1521, 1813
PleI	GAGTC	1 cut(s)	672
PpsI	GAGTC	1 cut(s)	672
PpuMI	RGGWCCY	1 cut(s)	7
PscI	ACATGT	1 cut(s)	738
PsiI	TTATAA	2 cut(s)	675, 1482
Psp5II	RGGWCCY	1 cut(s)	7
PspEI	GGTNACC	1 cut(s)	648
PspN4I	GGNNCC	2 cut(s)	8, 1134
PspPI	GGNCC	5 cut(s)	7, 23, 181, 413, 1556
PspPPI	RGGWCCY	1 cut(s)	7
PstI	CTGCAG	1 cut(s)	421
PstNI	CAGNNNCTG	1 cut(s)	1001
PsuI	RGATCY	3 cut(s)	505, 998, 1315
PvuII	CAGCTG	1 cut(s)	725
RsaI	GTAC	4 cut(s)	973, 1072, 1140, 1368
RsaNI	GTAC	4 cut(s)	972, 1071, 1139, 1367
RseI	CAYNNNNRTG	3 cut(s)	1233, 1406, 1410
SacII	CCGCGG	1 cut(s)	195
SapI	GCTCTTC	1 cut(s)	105
SaqAI	TTAA	5 cut(s)	692, 719, 1205, 1350, 1725
SatI	GCNGC	7 cut(s)	192, 420, 683, 767, 1424, 1520, 1812
Sau3AI	GATC	7 cut(s)	103, 136, 156, 223, 505, 998, 1315
Sau96I	GGNCC	5 cut(s)	7, 23, 181, 413, 1556
ScaI	AGTACT	1 cut(s)	1072
SchI	GAGTC	1 cut(s)	673
ScrFI	CCNGG	1 cut(s)	1506
SduI	GDGCHC	4 cut(s)	256, 1135, 1506, 1763
SfaNI	GCATC	9 cut(s)	451, 505, 537, 894, 982, 1225, 1443, 1465, 1660
SfcI	CTRYAG	1 cut(s)	417
Sfr303I	CCGCGG	1 cut(s)	195
SgrBI	CCGCGG	1 cut(s)	195
SinI	GGWCC	3 cut(s)	7, 23, 1556
SmiMI	CAYNNNNRTG	3 cut(s)	1233, 1406, 1410
SmlI	CTYRAG	1 cut(s)	55
SmoI	CTYRAG	1 cut(s)	55
SphI	GCATGC	2 cut(s)	935, 1405
SsiI	CCGC	6 cut(s)	192, 194, 354, 683, 766, 1421
SspI	AATATT	1 cut(s)	1282
SspMI	CTAG	1 cut(s)	1053
StyD4I	CCNGG	1 cut(s)	1504
StyI	CCWWGG	3 cut(s)	10, 605, 1747
TaaI	ACNGT	6 cut(s)	537, 940, 953, 1379, 1585, 1666
TaiI	ACGT	1 cut(s)	913
TaqI	TCGA	6 cut(s)	139, 232, 844, 862, 990, 1668
TatI	WGTACW	1 cut(s)	1070
TauI	GCSGC	3 cut(s)	194, 685, 769
TfiI	GAWTC	2 cut(s)	141, 445
Tru1I	TTAA	5 cut(s)	692, 719, 1205, 1350, 1725
Tru9I	TTAA	5 cut(s)	692, 719, 1205, 1350, 1725
TscAI	CASTG	6 cut(s)	94, 226, 560, 958, 1237, 1768
TseFI	GTSAC	3 cut(s)	89, 1175, 1288
TseI	GCWGC	4 cut(s)	419, 1423, 1519, 1811
Tsp45I	GTSAC	3 cut(s)	89, 1175, 1288
TspGWI	ACGGA	2 cut(s)	139, 764
TspRI	CASTG	6 cut(s)	94, 226, 560, 958, 1237, 1768
Van91I	CCANNNNNTGG	1 cut(s)	914
VpaK11BI	GGWCC	3 cut(s)	7, 23, 1556
XagI	CCTNNNNNAGG	1 cut(s)	14
XapI	RAATTY	1 cut(s)	264
XceI	RCATGY	6 cut(s)	742, 773, 935, 1196, 1405, 1409
XcmI	CCANNNNNNNNNTGG	1 cut(s)	606
XspI	CTAG	1 cut(s)	1053
ZrmI	AGTACT	1 cut(s)	1072
Zsp2I	ATGCAT	2 cut(s)	1407, 1458

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.