Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0008737

Physical Location & Seq

Genomic Coordinates Reverse (-)

1921853 .. 1926145

Gene Structure 4293 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1022400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1821 bp

ATGGGTGACGTTGGAGAGATTGGACCTGATATTAGTTCGGATATAGAAGAAGACTTGAGGTGCGATAATATTGCGGAGAAAGATGTCAGTGACGAAGAGATTGAAGCGGAAGATTTGGAGAGGCGAATGTGGAAGGATCGAATTAAACTCAAAAGGCTTAAAGAAAGACAGAAACTTGAAGCTCAACAAGCTGCTGAAAAGCAGAAGCCCAAGCAGACCACTGATCAGGCTAGGAGAAAGAAAATGTCAAGAGCACAAGATGGGATTCTTAAGTATATGTTGAAGCTGATGGAAGTGTGCAAAGCTCGTGGTTTTGTGTATGGTATCATTCCTGAGAAGGGCAAGCCAGTGAGCGGTGCTTCAGATAACATCAGGGCTTGGTGGAAAGAAAAGGTGAAGTTCGATAAGAATGGTCCTGCAGCCATAGCCAAGCATGAAGCGGAGTGTCTTGCCATGAGTGATGCAGATAATAATCGAAATGGGAATTCTCAAAGCATCCTCCAAGATCTACAAGATGCAACTCTTGGTTCTCTATTGTCTTCTTTGATGCAACACTGTGACCCCCCTCAAAGGAAGTATCCATTAGAAAAGGGAGTTCCACCACCTTGGTGGCCAACAGGAAATGAAGATTGGTGGGTGAAACTAGGGTTAGCCCATGGTCAGATTCCTCCTTATAAGAAGCCACACGACCTAAAGAAGATGTGGAAAGTTGGAGTGTTAACGGCTGTGATAAAGCATATGTCACCTGATATTGCAAAGATAAGGAGGCATGTCCGCCAGTCAAAATGCTTACAGGATAAGATGACAGCAAAGGAAAGTGCAATTTGGTTAGGGGTTTTAAGCCGAGAAGAATCCCTCATTCGGCAGCCTAGTAGTGATAATGGGACATCTGGCATTACTGAGATGCCACAAAGTGGCCATGGCGAAAAGCAAGGTGCTGCTAGCAGTAATAGTGACTATGATGTTGATGGTACTGATGATGGGGTAGGCTCTGTTTCTTCATCCAAAGATGATAGGAGGAATCAGGTGATGGATGTGGATCCATCAAGCAATCTACACAATAATGCTCGTAATAATGTCCAAGATAAAGAGCAAGGTGAAAAGAAACCCAGGAGAAAAAGGGCACGTGTCAGAGCAAGCCCTGTCAAACAACGTCCTGCACCATCTCACAATGAGCATCTTCATGTTCCAACAAGAGGTGCATTGCCTGATATAAACCACACTGATGTACAGATGATTGGACTTCAGGTTCATGAAAATCAACAGGAAAATGGTGCCATTACAACTTTGCGGCCGCTGGAGAATGATCTTGATGTCCAAGCACAAAGACCAGCGCCAGAGTTTAACTACCCTGGTGTACCTTCTGGCAATGTAATTACGACACAGGGCATGCATGTTGGTGGAACACCGTTGCTTTATCATGGGGTGCGAGATGCTGACATGCATCATGGAGATACATTTAACCAGCATCATGGAGATACATTTAACCTCTATAACCCATCAACACAATATCCCCCAAGTCATGACCGGCAGCCGCCTCAGATTGTAATGAATGAACCGCAGATTATACCAGCAGATGGAGTCCATGTACCAGTACATAGAAATGGAAGTGAAATTGCTGGAGGAGATTTGCCAAATTTTGTTCAGGACACATTTCAGAGTGAGCAAGACAGAACTATCAATGCTAACTTTGGGTCTCCAATTGATAGCCTGTCGTTAGATTATGGATTATTCAACAGCCCATTCAACTTTGGAATTGACGGCACCGGTTCATTAGATGACCTTGAACTCGATGAAATGATGGAGTACTTTGCAGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009889 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031326 GO:0032870 GO:0035556 GO:0042221 GO:0042762 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071369 GO:0071495 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 map04016 map04075

Pfam Domains

Protein Families

EIN3

Protein Analysis

607

Amino Acids

67.67

Weight (kDa)

5.9

Isoelectric Point (pI)

54.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EIN3_DNA-bd	PF04873	37 - 284	1.2e-128	Ethylene insensitive 3, DNA-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008737)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G73730 AT1G73730
fragaria_vesca	FvH4_3g43580 FvH4_3g43580
malus_domestica	MD03G1020800.v1.1 MD11G1022400.v1.1
prunus_persica	Prupe.6G018200_v2.0.a1 Prupe.6G018200_v2.0.a1
pyrus_communis	pycom03g01780 pycom11g01800
rosa_chinensis	RchiOBHm_Chr5g0078151
rosa_laevigata	RLG00000036758
rosa_multiflora	Rmu_sc0018349.1_g000004 Rmu_sc0018349.1_g000007
rosa_roxburghii	Rroxscaffold_1G00003980
rosa_rugosa	Rorug05G0459100 Rorug05G0459200 Rorug05G0459300
rosa_samantha	Rh5AG513600 Rh5BG536600 Rh5CG560000 Rh5DG548200
rosa_wichuraiana	Rw5G047660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	675
AccB1I	GGYRCC	2 cut(s)	1274, 1763
AccB7I	CCANNNNNTGG	2 cut(s)	914, 1577
AccBSI	CCGCTC	1 cut(s)	354
AciI	CCGC	9 cut(s)	74, 107, 354, 440, 775, 1291, 1295, 1535, 1559
AclWI	GGATC	3 cut(s)	144, 1034, 1047
AcoI	YGGCCR	3 cut(s)	611, 916, 1292
AcsI	RAATTY	2 cut(s)	484, 1636
AcuI	CTGAAG	2 cut(s)	345, 1229
AcvI	CACGTG	1 cut(s)	1127
AdeI	CACNNNGTG	1 cut(s)	914
AfaI	GTAC	6 cut(s)	973, 1230, 1359, 1590, 1596, 1808
AfiI	CCNNNNNNNGG	7 cut(s)	338, 353, 570, 861, 914, 1196, 1577
AflII	CTTAAG	1 cut(s)	269
AflIII	ACRYGT	1 cut(s)	1126
AgeI	ACCGGT	1 cut(s)	1766
AgsI	TTSAA	6 cut(s)	104, 179, 283, 1735, 1747, 1787
AjnI	CCWGG	2 cut(s)	1109, 1351
AjuI	GAANNNNNNNTTGG	2 cut(s)	613, 645
AleI	CACNNNNGTG	1 cut(s)	607
AloI	GAACNNNNNNTCC	2 cut(s)	1233, 1265
AluBI	AGCT	4 cut(s)	182, 191, 286, 305
AluI	AGCT	4 cut(s)	182, 191, 286, 305
Alw21I	GWGCWC	1 cut(s)	256
Alw26I	GTCTC	1 cut(s)	1701
AlwI	GGATC	3 cut(s)	144, 1034, 1047
AoxI	GGCC	3 cut(s)	611, 916, 1292
ApeKI	GCWGC	6 cut(s)	191, 419, 865, 938, 1531, 1814
ApoI	RAATTY	2 cut(s)	484, 1636
AsiGI	ACCGGT	1 cut(s)	1766
AspLEI	GCGC	1 cut(s)	1336
AspS9I	GGNCC	2 cut(s)	23, 413
AsuHPI	GGTGA	6 cut(s)	17, 406, 649, 735, 1039, 1109
AsuNHI	GCTAGC	1 cut(s)	941
AvaII	GGWCC	2 cut(s)	23, 413
BaeGI	GKGCMC	1 cut(s)	1126
BalI	TGGCCA	2 cut(s)	613, 918
BamHI	GGATCC	1 cut(s)	1039
BanI	GGYRCC	2 cut(s)	1274, 1763
BauI	CACGAG	1 cut(s)	306
BbrPI	CACGTG	1 cut(s)	1127
BbsI	GAAGAC	2 cut(s)	57, 531
Bbv12I	GWGCWC	1 cut(s)	256
BbvI	GCAGC	5 cut(s)	178, 431, 877, 925, 1543
BceAI	ACGGC	2 cut(s)	738, 1777
BcgI	CGANNNNNNTGC	2 cut(s)	53, 87
BciT130I	CCWGG	2 cut(s)	1111, 1353
BciVI	GTATCC	1 cut(s)	588
BclI	TGATCA	1 cut(s)	223
BcoDI	GTCTC	1 cut(s)	1701
BfaI	CTAG	4 cut(s)	231, 644, 870, 942
BfmI	CTRYAG	1 cut(s)	417
BfoI	RGCGCY	1 cut(s)	1337
BfrI	CTTAAG	1 cut(s)	269
BfuI	GTATCC	1 cut(s)	588
BglII	AGATCT	1 cut(s)	505
BisI	GCNGC	9 cut(s)	192, 420, 866, 939, 1292, 1295, 1532, 1535, 1815
BlsI	GCNGC	9 cut(s)	193, 421, 867, 940, 1293, 1296, 1533, 1536, 1816
BmcAI	AGTACT	1 cut(s)	1808
Bme1390I	CCNGG	2 cut(s)	1111, 1353
Bme18I	GGWCC	2 cut(s)	23, 413
BmgT120I	GGNCC	2 cut(s)	23, 413
BmiI	GGNNCC	3 cut(s)	1041, 1276, 1765
BmrFI	CCNGG	2 cut(s)	1111, 1353
BmsI	GCATC	9 cut(s)	451, 504, 505, 537, 894, 1186, 1423, 1453, 1477
BmtI	GCTAGC	1 cut(s)	945
BpiI	GAAGAC	2 cut(s)	57, 531
BpmI	CTGGAG	2 cut(s)	1319, 1641
BpuEI	CTTGAG	1 cut(s)	76
BsaAI	YACGTR	1 cut(s)	1127
BsaBI	GATNNNNATC	2 cut(s)	471, 1038
BsaI	GGTCTC	1 cut(s)	1701
BsaJI	CCNNGG	5 cut(s)	605, 655, 919, 1109, 1351
BsaWI	WCCGGW	1 cut(s)	1766
BsaXI	ACNNNNNCTCC	2 cut(s)	1572, 1602
Bsc4I	CCNNNNNNNGG	7 cut(s)	338, 353, 570, 861, 914, 1196, 1577
Bse118I	RCCGGY	2 cut(s)	1527, 1766
Bse1I	ACTGG	3 cut(s)	347, 778, 1592
Bse3DI	GCAATG	2 cut(s)	1202, 1375
Bse8I	GATNNNNATC	2 cut(s)	471, 1038
BseBI	CCWGG	2 cut(s)	1111, 1353
BseDI	CCNNGG	5 cut(s)	605, 655, 919, 1109, 1351
BseGI	GGATG	3 cut(s)	495, 1001, 1039
BseJI	GATNNNNATC	2 cut(s)	471, 1038
BseLI	CCNNNNNNNGG	7 cut(s)	338, 353, 570, 861, 914, 1196, 1577
BseMI	GCAATG	2 cut(s)	1202, 1375
BseMII	CTCAG	3 cut(s)	324, 891, 1553
BseNI	ACTGG	3 cut(s)	347, 778, 1592
BseRI	GAGGAG	1 cut(s)	1638
BseSI	GKGCMC	1 cut(s)	1126
BseX3I	CGGCCG	1 cut(s)	1292
BseXI	GCAGC	5 cut(s)	178, 431, 877, 925, 1543
BsgI	GTGCAG	1 cut(s)	1143
Bsh1285I	CGRYCG	1 cut(s)	1295
BshFI	GGCC	3 cut(s)	613, 918, 1294
BshNI	GGYRCC	2 cut(s)	1274, 1763
BshTI	ACCGGT	1 cut(s)	1766
BsiEI	CGRYCG	1 cut(s)	1295
BsiHKAI	GWGCWC	1 cut(s)	256
BsiSI	CCGG	2 cut(s)	1528, 1767
BslFI	GGGAC	1 cut(s)	898
BslI	CCNNNNNNNGG	7 cut(s)	338, 353, 570, 861, 914, 1196, 1577
BsmAI	GTCTC	1 cut(s)	1701
BsmFI	GGGAC	1 cut(s)	898
BsnI	GGCC	3 cut(s)	613, 918, 1294
Bso31I	GGTCTC	1 cut(s)	1701
Bsp1286I	GDGCHC	2 cut(s)	256, 1126
Bsp1407I	TGTACA	1 cut(s)	1228
Bsp143I	GATC	5 cut(s)	136, 223, 505, 1039, 1306
Bsp19I	CCATGG	2 cut(s)	655, 919
BspACI	CCGC	9 cut(s)	74, 107, 354, 440, 775, 1291, 1295, 1535, 1559
BspANI	GGCC	3 cut(s)	613, 918, 1294
BspCNI	CTCAG	3 cut(s)	325, 892, 1552
BspHI	TCATGA	2 cut(s)	1252, 1522
BspLI	GGNNCC	3 cut(s)	1041, 1276, 1765
BspMAI	CTGCAG	1 cut(s)	421
BspOI	GCTAGC	1 cut(s)	945
BspPI	GGATC	3 cut(s)	144, 1034, 1047
BspT107I	GGYRCC	2 cut(s)	1274, 1763
BspTI	CTTAAG	1 cut(s)	269
BspTNI	GGTCTC	1 cut(s)	1701
BsrBI	CCGCTC	1 cut(s)	354
BsrDI	GCAATG	2 cut(s)	1202, 1375
BsrFI	RCCGGY	2 cut(s)	1527, 1766
BsrGI	TGTACA	1 cut(s)	1228
BsrI	ACTGG	3 cut(s)	347, 778, 1592
BssAI	RCCGGY	2 cut(s)	1527, 1766
BssECI	CCNNGG	5 cut(s)	605, 655, 919, 1109, 1351
BssMI	GATC	5 cut(s)	136, 223, 505, 1039, 1306
BssSI	CACGAG	1 cut(s)	306
BssT1I	CCWWGG	3 cut(s)	605, 655, 919
Bst2BI	CACGAG	1 cut(s)	306
Bst2UI	CCWGG	2 cut(s)	1111, 1353
Bst4CI	ACNGT	2 cut(s)	557, 1410
Bst6I	CTCTTC	1 cut(s)	90
BstAFI	CTTAAG	1 cut(s)	269
BstAUI	TGTACA	1 cut(s)	1228
BstBAI	YACGTR	1 cut(s)	1127
BstC8I	GCNNGC	4 cut(s)	344, 943, 1138, 1391
BstDEI	CTNAG	3 cut(s)	333, 900, 1539
BstDSI	CCRYGG	2 cut(s)	655, 919
BstF5I	GGATG	3 cut(s)	495, 1001, 1039
BstH2I	RGCGCY	1 cut(s)	1337
BstHHI	GCGC	1 cut(s)	1336
BstKTI	GATC	5 cut(s)	139, 226, 508, 1042, 1309
BstMAI	GTCTC	1 cut(s)	1701
BstMBI	GATC	5 cut(s)	136, 223, 505, 1039, 1306
BstMCI	CGRYCG	1 cut(s)	1295
BstMWI	GCNNNNNNNGC	2 cut(s)	188, 425
BstNI	CCWGG	2 cut(s)	1111, 1353
BstNSI	RCATGY	4 cut(s)	773, 1393, 1397, 1444
BstSCI	CCNGG	2 cut(s)	1109, 1351
BstSFI	CTRYAG	1 cut(s)	417
BstSLI	GKGCMC	1 cut(s)	1126
BstV1I	GCAGC	5 cut(s)	178, 431, 877, 925, 1543
BstV2I	GAAGAC	2 cut(s)	57, 531
BstX2I	RGATCY	2 cut(s)	505, 1039
BstXI	CCANNNNNNTGG	3 cut(s)	606, 609, 1472
BstYI	RGATCY	2 cut(s)	505, 1039
BstZI	CGGCCG	1 cut(s)	1292
BsuI	GTATCC	1 cut(s)	588
BsuRI	GGCC	3 cut(s)	613, 918, 1294
BtgI	CCRYGG	2 cut(s)	655, 919
BtsCI	GGATG	3 cut(s)	495, 1001, 1039
BtsIMutI	CAGTG	5 cut(s)	94, 219, 354, 553, 1221
Cac8I	GCNNGC	4 cut(s)	344, 943, 1138, 1391
CciI	TCATGA	2 cut(s)	1252, 1522
CciNI	GCGGCCGC	1 cut(s)	1292
CfoI	GCGC	1 cut(s)	1336
Cfr10I	RCCGGY	2 cut(s)	1527, 1766
Cfr13I	GGNCC	2 cut(s)	23, 413
Csp6I	GTAC	6 cut(s)	972, 1229, 1358, 1589, 1595, 1807
CspAI	ACCGGT	1 cut(s)	1766
CspCI	CAANNNNNGTGG	2 cut(s)	289, 324
CviQI	GTAC	6 cut(s)	972, 1229, 1358, 1589, 1595, 1807
DdeI	CTNAG	3 cut(s)	333, 900, 1539
DpnI	GATC	5 cut(s)	138, 225, 507, 1041, 1308
DpnII	GATC	5 cut(s)	136, 223, 505, 1039, 1306
DraIII	CACNNNGTG	1 cut(s)	914
EaeI	YGGCCR	3 cut(s)	611, 916, 1292
EagI	CGGCCG	1 cut(s)	1292
Eam1104I	CTCTTC	1 cut(s)	90
EarI	CTCTTC	1 cut(s)	90
EciI	GGCGGA	1 cut(s)	764
EclXI	CGGCCG	1 cut(s)	1292
Eco130I	CCWWGG	3 cut(s)	605, 655, 919
Eco31I	GGTCTC	1 cut(s)	1701
Eco47I	GGWCC	2 cut(s)	23, 413
Eco52I	CGGCCG	1 cut(s)	1292
Eco57I	CTGAAG	2 cut(s)	345, 1229
Eco72I	CACGTG	1 cut(s)	1127
EcoRI	GAATTC	1 cut(s)	484
EcoRII	CCWGG	2 cut(s)	1109, 1351
EcoT14I	CCWWGG	3 cut(s)	605, 655, 919
EcoT22I	ATGCAT	2 cut(s)	1395, 1446
ErhI	CCWWGG	3 cut(s)	605, 655, 919
FaqI	GGGAC	1 cut(s)	898
FauNDI	CATATG	1 cut(s)	738
FbaI	TGATCA	1 cut(s)	223
Fnu4HI	GCNGC	9 cut(s)	192, 420, 866, 939, 1292, 1295, 1532, 1535, 1815
FokI	GGATG	3 cut(s)	482, 988, 1046
Fsp4HI	GCNGC	9 cut(s)	192, 420, 866, 939, 1292, 1295, 1532, 1535, 1815
FspBI	CTAG	4 cut(s)	231, 644, 870, 942
GlaI	GCGC	1 cut(s)	1335
GluI	GCNGC	9 cut(s)	192, 420, 866, 939, 1292, 1295, 1532, 1535, 1815
GsuI	CTGGAG	2 cut(s)	1319, 1641
HaeII	RGCGCY	1 cut(s)	1337
HaeIII	GGCC	3 cut(s)	613, 918, 1294
HapII	CCGG	2 cut(s)	1528, 1767
HhaI	GCGC	1 cut(s)	1336
Hin6I	GCGC	1 cut(s)	1334
HinP1I	GCGC	1 cut(s)	1334
HincII	GTYRAC	1 cut(s)	720
HindII	GTYRAC	1 cut(s)	720
HinfI	GANTC	5 cut(s)	265, 664, 851, 1021, 1581
HpaI	GTTAAC	1 cut(s)	720
HpaII	CCGG	2 cut(s)	1528, 1767
HphI	GGTGA	6 cut(s)	17, 406, 649, 735, 1039, 1109
Hpy166II	GTNNAC	2 cut(s)	720, 1358
Hpy188I	TCNGA	6 cut(s)	40, 364, 663, 1133, 1542, 1659
Hpy188III	TCNNGA	6 cut(s)	249, 332, 1253, 1310, 1523, 1646
Hpy8I	GTNNAC	2 cut(s)	720, 1358
HpyAV	CCTTC	3 cut(s)	127, 331, 1371
HpyCH4III	ACNGT	2 cut(s)	557, 1410
HpyCH4IV	ACGT	3 cut(s)	9, 1126, 1153
HpyF10VI	GCNNNNNNNGC	2 cut(s)	188, 425
HpyF3I	CTNAG	3 cut(s)	333, 900, 1539
HpySE526I	ACGT	3 cut(s)	9, 1126, 1153
HspAI	GCGC	1 cut(s)	1334
Ksp22I	TGATCA	1 cut(s)	223
KspAI	GTTAAC	1 cut(s)	720
Kzo9I	GATC	5 cut(s)	136, 223, 505, 1039, 1306
Lsp1109I	GCAGC	5 cut(s)	178, 431, 877, 925, 1543
LweI	GCATC	9 cut(s)	451, 504, 505, 537, 894, 1186, 1423, 1453, 1477
MaeI	CTAG	4 cut(s)	231, 644, 870, 942
MaeII	ACGT	3 cut(s)	9, 1126, 1153
MaeIII	GTNAC	5 cut(s)	5, 89, 557, 741, 953
MalI	GATC	5 cut(s)	138, 225, 507, 1041, 1308
MbiI	CCGCTC	1 cut(s)	354
MboI	GATC	5 cut(s)	136, 223, 505, 1039, 1306
MfeI	CAATTG	1 cut(s)	1701
MflI	RGATCY	2 cut(s)	505, 1039
MhlI	GDGCHC	2 cut(s)	256, 1126
MlsI	TGGCCA	2 cut(s)	613, 918
MluCI	AATT	8 cut(s)	141, 484, 822, 1374, 1614, 1636, 1701, 1755
MluNI	TGGCCA	2 cut(s)	613, 918
MlyI	GAGTC	1 cut(s)	1590
MmeI	TCCRAC	2 cut(s)	691, 1214
Mox20I	TGGCCA	2 cut(s)	613, 918
Mph1103I	ATGCAT	2 cut(s)	1395, 1446
MscI	TGGCCA	2 cut(s)	613, 918
MseI	TTAA	8 cut(s)	144, 159, 270, 719, 839, 1344, 1461, 1485
MslI	CAYNNNNRTG	6 cut(s)	607, 1062, 1182, 1224, 1398, 1602
Msp20I	TGGCCA	2 cut(s)	613, 918
MspA1I	CMGCKG	1 cut(s)	1297
MspCI	CTTAAG	1 cut(s)	269
MspI	CCGG	2 cut(s)	1528, 1767
MspR9I	CCNGG	2 cut(s)	1111, 1353
MunI	CAATTG	1 cut(s)	1701
MvaI	CCWGG	2 cut(s)	1111, 1353
MwoI	GCNNNNNNNGC	2 cut(s)	188, 425
NcoI	CCATGG	2 cut(s)	655, 919
NdeI	CATATG	1 cut(s)	738
NdeII	GATC	5 cut(s)	136, 223, 505, 1039, 1306
NheI	GCTAGC	1 cut(s)	941
NlaIV	GGNNCC	3 cut(s)	1041, 1276, 1765
NmeAIII	GCCGAG	1 cut(s)	869
NmuCI	GTSAC	5 cut(s)	5, 89, 557, 741, 953
NotI	GCGGCCGC	1 cut(s)	1292
NsiI	ATGCAT	2 cut(s)	1395, 1446
NspI	RCATGY	4 cut(s)	773, 1393, 1397, 1444
OliI	CACNNNNGTG	1 cut(s)	607
PaeI	GCATGC	1 cut(s)	1393
PagI	TCATGA	2 cut(s)	1252, 1522
PfeI	GAWTC	4 cut(s)	265, 664, 851, 1021
PflMI	CCANNNNNTGG	2 cut(s)	914, 1577
PinAI	ACCGGT	1 cut(s)	1766
PkrI	GCNGC	9 cut(s)	193, 421, 867, 940, 1293, 1296, 1533, 1536, 1816
PleI	GAGTC	1 cut(s)	1589
PmaCI	CACGTG	1 cut(s)	1127
PmlI	CACGTG	1 cut(s)	1127
PpsI	GAGTC	1 cut(s)	1589
Ppu21I	YACGTR	1 cut(s)	1127
PsiI	TTATAA	1 cut(s)	675
Psp6I	CCWGG	2 cut(s)	1109, 1351
PspCI	CACGTG	1 cut(s)	1127
PspGI	CCWGG	2 cut(s)	1109, 1351
PspN4I	GGNNCC	3 cut(s)	1041, 1276, 1765
PspPI	GGNCC	2 cut(s)	23, 413
PstI	CTGCAG	1 cut(s)	421
PsuI	RGATCY	2 cut(s)	505, 1039
RsaI	GTAC	6 cut(s)	973, 1230, 1359, 1590, 1596, 1808
RsaNI	GTAC	6 cut(s)	972, 1229, 1358, 1589, 1595, 1807
RseI	CAYNNNNRTG	6 cut(s)	607, 1062, 1182, 1224, 1398, 1602
SaqAI	TTAA	8 cut(s)	144, 159, 270, 719, 839, 1344, 1461, 1485
SatI	GCNGC	9 cut(s)	192, 420, 866, 939, 1292, 1295, 1532, 1535, 1815
Sau3AI	GATC	5 cut(s)	136, 223, 505, 1039, 1306
Sau96I	GGNCC	2 cut(s)	23, 413
ScaI	AGTACT	1 cut(s)	1808
SchI	GAGTC	1 cut(s)	1590
ScrFI	CCNGG	2 cut(s)	1111, 1353
SduI	GDGCHC	2 cut(s)	256, 1126
SfaNI	GCATC	9 cut(s)	451, 504, 505, 537, 894, 1186, 1423, 1453, 1477
SfcI	CTRYAG	1 cut(s)	417
SinI	GGWCC	2 cut(s)	23, 413
SmiMI	CAYNNNNRTG	6 cut(s)	607, 1062, 1182, 1224, 1398, 1602
SmlI	CTYRAG	2 cut(s)	55, 269
SmoI	CTYRAG	2 cut(s)	55, 269
SphI	GCATGC	1 cut(s)	1393
Sse9I	AATT	8 cut(s)	141, 484, 822, 1374, 1614, 1636, 1701, 1755
SsiI	CCGC	9 cut(s)	74, 107, 354, 440, 775, 1291, 1295, 1535, 1559
SspI	AATATT	1 cut(s)	70
SspMI	CTAG	4 cut(s)	231, 644, 870, 942
StyD4I	CCNGG	2 cut(s)	1109, 1351
StyI	CCWWGG	3 cut(s)	605, 655, 919
TaaI	ACNGT	2 cut(s)	557, 1410
TaiI	ACGT	3 cut(s)	12, 1129, 1156
TaqI	TCGA	4 cut(s)	139, 402, 475, 1791
TasI	AATT	8 cut(s)	141, 484, 822, 1374, 1614, 1636, 1701, 1755
TatI	WGTACW	3 cut(s)	1228, 1594, 1806
TauI	GCSGC	3 cut(s)	1294, 1297, 1537
TfiI	GAWTC	4 cut(s)	265, 664, 851, 1021
Tru1I	TTAA	8 cut(s)	144, 159, 270, 719, 839, 1344, 1461, 1485
Tru9I	TTAA	8 cut(s)	144, 159, 270, 719, 839, 1344, 1461, 1485
TscAI	CASTG	5 cut(s)	94, 226, 354, 560, 1228
TseFI	GTSAC	5 cut(s)	5, 89, 557, 741, 953
TseI	GCWGC	6 cut(s)	191, 419, 865, 938, 1531, 1814
Tsp45I	GTSAC	5 cut(s)	5, 89, 557, 741, 953
TspRI	CASTG	5 cut(s)	94, 226, 354, 560, 1228
Van91I	CCANNNNNTGG	2 cut(s)	914, 1577
Vha464I	CTTAAG	1 cut(s)	269
VpaK11BI	GGWCC	2 cut(s)	23, 413
XapI	RAATTY	2 cut(s)	484, 1636
XceI	RCATGY	4 cut(s)	773, 1393, 1397, 1444
XcmI	CCANNNNNNNNNTGG	1 cut(s)	606
XspI	CTAG	4 cut(s)	231, 644, 870, 942
ZrmI	AGTACT	1 cut(s)	1808
Zsp2I	ATGCAT	2 cut(s)	1395, 1446

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.