Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0008013

Physical Location & Seq

Genomic Coordinates Forward (+)

3503000 .. 3508630

Gene Structure 5631 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1044300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2400 bp

ATGTCAGGCGAGGAATACAGCGATGAGACTCGTCTGCTGCGATCCTCCAGCGACATTATCAAAGACGACAACGCCGGCGTTTCGGCAGAGAAAGACAGCGACTTGGAAGCTCAAGAGGGATTAATAAGCTGCAGGAGAAGCAGTATTAGTCCGACCAGTAGAAGAGGCGGAGTCGGAGGCTTCAAAGATCTGCTGATCAAGCATTTGGACGGCGTAGGGGGGCTTTCCGGCCGCCGTCTCAGTATCAAGCGCGGCCGAGATAATTCCCCAAATCACGGCGGCAGAGAGGTTAGAGGTCAGCTTTCCGCTGTAGATCACCACCACCATCATAACCAGCATCATCAGATTCAGCAGCAGCATCATGAGTCTCCGGCCGGAAGCGACGGTAATGACGAGCTTGCCGATAGTGCCCCACCGGAGTGGGCGTTGCTTCTCCTTGGTTGCCTTCTTGGCCTCGCCACGGGTCTCTTTGTTGCCGCATTTAACAAAGGGGTACATGTTATACATGAATGGGCCTGGGCTGGTACTCCAAGTGAAGGTGCTGCATGGCTCCGTCTGCAGAGACTGGGCGATACCTGGCATCGAATTCTCCTAATACCGGTCACGGGTGGGGTTATTGTTGGCATGATGCATGGTTTACTTGAAATATTGGACCAGATAAGGCAGTCCAGTTCCTCTCAAAGACAAGGATTTGATTTACTTGCTGCAGTCTTTCCCTCAATAAAGGCTGTCCAAGCAGCTGTCACTTTAGGTACCGGTTGTTCTTTGGGTCCTGAAGGCCCTAGTGTAGATATTGGAAAATCTTGTGCCAATGGATTCTCATTAATGATGGAAAACAACAGAGAAAGGAAGATTGCCCTTGTTGCAGCAGGTGCAGCGGCCGGAATTTCTTCAGGTTTCAACGCGGCAGTTGCTGGTTGTTTCTTCGCTATTGAAACGGTGTTAAGGCCTCTTCGAGCAGAAAACTCACCTCCATTCACAACTGCAATGATTATTTTGGCTTCTGTTATATCATCTACTGTATCTAATGTTTTACAAGGGACTCAATCAGCGTTTACAGTGCCTACATACGATCTAAAATCTGCTGCTGAACTTCCTCTATACCTGATATTGGGAATGCTATGTGGTGCTGTAAGTGTAGCCTTGACTCGATTGGTTGCTTGGTTCACAAAGTTCTTTGATTTTATCAAGGAAAGATTTGGTCTTCCAGCTGTTGCCTGCCCAGCTTTAGGTGGTTTAGGAGCTGGGATTATTGCTCTTAAGTATCCTGGAATTCTGTACTGGGGTTTTACAAATGTTGAAGAAATTTTGCATACTGGGAATGCTTCAGCTCCTGGAATCTGGCTGTTAACTCAGTTAGCAGCAGCTAAAGTTGTGGCAACAGCTTTATGCAAGGGTTCTGGGTTGGTAGGTGGCCTATACGCACCAAGCTTGATGATTGGTGCTGCTGTTGGTGCTGTATTTGGGGGTTCGGCTGCAGAACTGATAAACTCAGCGATTCCAGGAAATGCTGCTGTTGCCCAGCCACAGGCTTATGCACTGGTTGGAATGGCTGCTACACTAGCTTCAGTTTGTTCAGTACCATTGACGTCAGTTCTGCTTCTGTTTGAGCTCACAAAAGATTACCGGATATTGCTCCCTCTCATGGGGGCTGTTGGATTGGCGATATGGGTGCCCTCTGTGGTAAACCAGGAAAAGGAGACTGAACCATCTGAAACACGGAATTCAGCAAGAGTTTATTCTTATGTTTCGGCTGATGAAGAAAAAGATGAAGCTAATTGGAGACAACATGATGATGGAGATGATTTGGAACTCTCTGTTATTGGAAACAATTCTGACTCCGAATTAGTTAGTGAAGAATTGCTTCTGGAAGATCTAAAGGTTTCGCGAGCCATGTCAAAGAAATATGTGAAGGTTCCTGTGAGCTTGACTCTGAAAGAGGCAATAAAATGCATGCATGAGAGTCACCAGAATTGTGTGTTGGTGGTTGATGATGAAGATTTTCTGGAGGGAATATTAACATATGGTGATGTTAGACGGTTTCAATCAAAGAAATCTAGCGATGCATCAAAGAGCGAATCTAGGTTTTTGGATGCAAATACATGTCTTGTTTCATCTGTTTGTACTCGAGGAATTCGCTATAGAGGAAGAGCGCGTGGTTTATTTACCTGTTATCCGGACACAGATTTAGCAATGGCCAAGGAGCTAATGGAGGCCAAGGATGTAAGGCAGTTGCCTGTGGTTAAGCGTGTCAGAGAACCACCAAAAGAAATAAAGCGAAGAATCGTTGCTATTCTTCATTACGATTCAATCTTGAACTGCCTCAGAGAGGAGATAAATCACAGGAAAACAGTTCAGCATAGAAAAGAGAATCTCCACGAGATTACCAATGGCCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0009507 GO:0009536 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS Voltage_CLC

Protein Analysis

800

Amino Acids

86.04

Weight (kDa)

6.24

Isoelectric Point (pI)

44.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Voltage_CLC	PF00654	227 - 558	1.8e-68	Voltage gated chloride channel
CBS	PF00571	628 - 682	6.2e-09	CBS domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008013)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55620 AT1G55620
fragaria_vesca	FvH4_3g41470 FvH4_3g41470 FvH4_3g41470 FvH4_3g41470 FvH4_3g41470 FvH4_3g41470 FvH4_3g41470
malus_domestica	MD03G1044300.v1.1 MD11G1047300.v1.1
prunus_persica	Prupe.6G036200_v2.0.a1
pyrus_communis	pycom03g03420 pycom11g03830
rosa_chinensis	RchiOBHm_Chr5g0074431
rosa_laevigata	RLG00000036476
rosa_multiflora	Rmu_sc0000573.1_g000001 Rmu_sc0025084.1_g000002
rosa_roxburghii	Rroxscaffold_1G00007090
rosa_rugosa	Rorug05G0431800
rosa_samantha	Rh5BG509800 Rh5CG533600 Rh5DG522700
rosa_wichuraiana	Rw5G045340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	860
AatII	GACGTC	1 cut(s)	1592
Acc36I	ACCTGC	1 cut(s)	860
Acc65I	GGTACC	1 cut(s)	752
AccB1I	GGYRCC	2 cut(s)	752, 1672
AccII	CGCG	4 cut(s)	252, 905, 1888, 2155
AccIII	TCCGGA	1 cut(s)	2176
AciI	CCGC	8 cut(s)	168, 232, 252, 279, 306, 477, 878, 905
AclWI	GGATC	1 cut(s)	36
AcoI	YGGCCR	5 cut(s)	229, 253, 372, 879, 2196
AcsI	RAATTY	6 cut(s)	585, 885, 1272, 1305, 1723, 2133
AcuI	CTGAAG	4 cut(s)	795, 876, 1311, 1551
AcyI	GRCGYC	1 cut(s)	1589
AfaI	GTAC	6 cut(s)	495, 526, 754, 1280, 1581, 2125
AflII	CTTAAG	1 cut(s)	1259
AflIII	ACRYGT	2 cut(s)	496, 2102
AgeI	ACCGGT	2 cut(s)	598, 755
AgsI	TTSAA	8 cut(s)	184, 644, 901, 935, 1301, 2045, 2310, 2317
AjnI	CCWGG	6 cut(s)	515, 575, 1267, 1333, 1501, 1689
AjuI	GAANNNNNNNTTGG	2 cut(s)	1964, 1996
AleI	CACNNNNGTG	1 cut(s)	418
Alw21I	GWGCWC	1 cut(s)	1614
Alw26I	GTCTC	7 cut(s)	20, 242, 372, 470, 556, 1694, 1777
AlwI	GGATC	1 cut(s)	36
AlwNI	CAGNNNCTG	3 cut(s)	565, 914, 1334
Ama87I	CYCGRG	1 cut(s)	2127
Aor13HI	TCCGGA	1 cut(s)	2176
ApoI	RAATTY	6 cut(s)	585, 885, 1272, 1305, 1723, 2133
AseI	ATTAAT	2 cut(s)	122, 824
AsiGI	ACCGGT	2 cut(s)	598, 755
Asp700I	GAANNNNTTC	4 cut(s)	889, 1831, 1863, 2001
Asp718I	GGTACC	1 cut(s)	752
AspLEI	GCGC	2 cut(s)	252, 2155
AspS9I	GGNCC	4 cut(s)	513, 652, 770, 779
AsuHPI	GGTGA	4 cut(s)	308, 960, 1958, 2039
AvaI	CYCGRG	1 cut(s)	2127
AvaII	GGWCC	2 cut(s)	652, 770
BaeGI	GKGCMC	2 cut(s)	412, 1677
BalI	TGGCCA	1 cut(s)	2198
BanI	GGYRCC	2 cut(s)	752, 1672
BanII	GRGCYC	1 cut(s)	1614
BauI	CACGAG	1 cut(s)	2378
BbsI	GAAGAC	1 cut(s)	1196
Bbv12I	GWGCWC	1 cut(s)	1614
BccI	CCATC	4 cut(s)	333, 823, 1717, 1790
BceAI	ACGGC	3 cut(s)	219, 226, 292
BcgI	CGANNNNNNTGC	2 cut(s)	1654, 1688
BciT130I	CCWGG	6 cut(s)	517, 577, 1269, 1335, 1503, 1691
BciVI	GTATCC	1 cut(s)	1275
BclI	TGATCA	1 cut(s)	195
BcoDI	GTCTC	7 cut(s)	20, 242, 372, 470, 556, 1694, 1777
BfaI	CTAG	4 cut(s)	783, 1562, 2058, 2082
BfmI	CTRYAG	6 cut(s)	130, 309, 557, 705, 1476, 2140
BfrI	CTTAAG	1 cut(s)	1259
BfuAI	ACCTGC	1 cut(s)	860
BfuI	GTATCC	1 cut(s)	1275
BglI	GCCNNNNNGGC	1 cut(s)	450
BglII	AGATCT	2 cut(s)	187, 1873
Bme1390I	CCNGG	6 cut(s)	517, 577, 1269, 1335, 1503, 1691
Bme18I	GGWCC	2 cut(s)	652, 770
BmeT110I	CYCGRG	1 cut(s)	2127
BmgT120I	GGNCC	4 cut(s)	513, 652, 770, 779
BmiI	GGNNCC	5 cut(s)	551, 754, 771, 1674, 1917
BmrFI	CCNGG	6 cut(s)	517, 577, 1269, 1335, 1503, 1691
BmrI	ACTGGG	3 cut(s)	575, 1291, 1326
BmsI	GCATC	7 cut(s)	346, 367, 589, 618, 2053, 2075, 2083
BmuI	ACTGGG	3 cut(s)	575, 1291, 1326
BpiI	GAAGAC	1 cut(s)	1196
BplI	GAGNNNNNCTC	2 cut(s)	1915, 1947
BpmI	CTGGAG	2 cut(s)	31, 2027
BpuEI	CTTGAG	1 cut(s)	96
BsaHI	GRCGYC	1 cut(s)	1589
BsaI	GGTCTC	1 cut(s)	470
BsaJI	CCNNGG	5 cut(s)	436, 459, 516, 2199, 2217
BsaWI	WCCGGW	5 cut(s)	415, 598, 755, 1626, 2176
BsaXI	ACNNNNNCTCC	4 cut(s)	127, 157, 410, 440
Bse118I	RCCGGY	3 cut(s)	74, 598, 755
Bse1I	ACTGG	6 cut(s)	156, 570, 669, 1286, 1321, 1545
Bse3DI	GCAATG	2 cut(s)	993, 2199
BseAI	TCCGGA	1 cut(s)	2176
BseBI	CCWGG	6 cut(s)	517, 577, 1269, 1335, 1503, 1691
BseDI	CCNNGG	5 cut(s)	436, 459, 516, 2199, 2217
BseGI	GGATG	2 cut(s)	2098, 2227
BseMI	GCAATG	2 cut(s)	993, 2199
BseMII	CTCAG	4 cut(s)	253, 1367, 1506, 2338
BseNI	ACTGG	6 cut(s)	156, 570, 669, 1286, 1321, 1545
BseRI	GAGGAG	1 cut(s)	2345
BseSI	GKGCMC	2 cut(s)	412, 1677
BseX3I	CGGCCG	4 cut(s)	229, 253, 372, 879
BseYI	CCCAGC	3 cut(s)	1222, 1244, 1521
BsgI	GTGCAG	1 cut(s)	894
Bsh1236I	CGCG	4 cut(s)	252, 905, 1888, 2155
Bsh1285I	CGRYCG	4 cut(s)	232, 256, 375, 882
BshNI	GGYRCC	2 cut(s)	752, 1672
BshTI	ACCGGT	2 cut(s)	598, 755
BsiEI	CGRYCG	4 cut(s)	232, 256, 375, 882
BsiHKAI	GWGCWC	1 cut(s)	1614
BsiHKCI	CYCGRG	1 cut(s)	2127
BslFI	GGGAC	1 cut(s)	1054
BsmAI	GTCTC	7 cut(s)	20, 242, 372, 470, 556, 1694, 1777
BsmBI	CGTCTC	1 cut(s)	242
BsmFI	GGGAC	1 cut(s)	1054
BsmI	GAATGC	2 cut(s)	1122, 1327
Bso31I	GGTCTC	1 cut(s)	470
BsoBI	CYCGRG	1 cut(s)	2127
Bsp1286I	GDGCHC	3 cut(s)	412, 1614, 1677
Bsp13I	TCCGGA	1 cut(s)	2176
Bsp143I	GATC	6 cut(s)	41, 187, 195, 313, 1072, 1873
Bsp68I	TCGCGA	1 cut(s)	1888
BspACI	CCGC	8 cut(s)	168, 232, 252, 279, 306, 477, 878, 905
BspCNI	CTCAG	4 cut(s)	252, 1366, 1505, 2337
BspEI	TCCGGA	1 cut(s)	2176
BspFNI	CGCG	4 cut(s)	252, 905, 1888, 2155
BspHI	TCATGA	1 cut(s)	361
BspLI	GGNNCC	5 cut(s)	551, 754, 771, 1674, 1917
BspMAI	CTGCAG	4 cut(s)	134, 561, 709, 1480
BspMI	ACCTGC	1 cut(s)	860
BspPI	GGATC	1 cut(s)	36
BspQI	GCTCTTC	1 cut(s)	2143
BspT107I	GGYRCC	2 cut(s)	752, 1672
BspTI	CTTAAG	1 cut(s)	1259
BspTNI	GGTCTC	1 cut(s)	470
BsrDI	GCAATG	2 cut(s)	993, 2199
BsrFI	RCCGGY	3 cut(s)	74, 598, 755
BsrI	ACTGG	6 cut(s)	156, 570, 669, 1286, 1321, 1545
BssAI	RCCGGY	3 cut(s)	74, 598, 755
BssECI	CCNNGG	5 cut(s)	436, 459, 516, 2199, 2217
BssMI	GATC	6 cut(s)	41, 187, 195, 313, 1072, 1873
BssNI	GRCGYC	1 cut(s)	1589
BssSI	CACGAG	1 cut(s)	2378
BssT1I	CCWWGG	3 cut(s)	436, 2199, 2217
Bst2BI	CACGAG	1 cut(s)	2378
Bst2UI	CCWGG	6 cut(s)	517, 577, 1269, 1335, 1503, 1691
Bst4CI	ACNGT	6 cut(s)	386, 940, 1021, 1060, 2040, 2353
Bst6I	CTCTTC	3 cut(s)	157, 957, 2143
BstACI	GRCGYC	1 cut(s)	1589
BstAFI	CTTAAG	1 cut(s)	1259
BstAPI	GCANNNNNTGC	1 cut(s)	872
BstC8I	GCNNGC	5 cut(s)	76, 399, 1219, 1890, 1955
BstDEI	CTNAG	4 cut(s)	239, 1353, 1492, 2324
BstDSI	CCRYGG	1 cut(s)	459
BstENI	CCTNNNNNAGG	1 cut(s)	2327
BstF5I	GGATG	2 cut(s)	2098, 2227
BstFNI	CGCG	4 cut(s)	252, 905, 1888, 2155
BstHHI	GCGC	2 cut(s)	252, 2155
BstKTI	GATC	6 cut(s)	44, 190, 198, 316, 1075, 1876
BstMAI	GTCTC	7 cut(s)	20, 242, 372, 470, 556, 1694, 1777
BstMBI	GATC	6 cut(s)	41, 187, 195, 313, 1072, 1873
BstMCI	CGRYCG	4 cut(s)	232, 256, 375, 882
BstNI	CCWGG	6 cut(s)	517, 577, 1269, 1335, 1503, 1691
BstNSI	RCATGY	3 cut(s)	500, 1957, 2106
BstSCI	CCNGG	6 cut(s)	515, 575, 1267, 1333, 1501, 1689
BstSFI	CTRYAG	6 cut(s)	130, 309, 557, 705, 1476, 2140
BstSLI	GKGCMC	2 cut(s)	412, 1677
BstUI	CGCG	4 cut(s)	252, 905, 1888, 2155
BstV2I	GAAGAC	1 cut(s)	1196
BstX2I	RGATCY	2 cut(s)	187, 1873
BstXI	CCANNNNNNTGG	1 cut(s)	420
BstYI	RGATCY	2 cut(s)	187, 1873
BstZI	CGGCCG	4 cut(s)	229, 253, 372, 879
BsuI	GTATCC	1 cut(s)	1275
BtgI	CCRYGG	1 cut(s)	459
BtgZI	GCGATG	2 cut(s)	36, 2076
BtsCI	GGATG	2 cut(s)	2098, 2227
BtsIMutI	CAGTG	2 cut(s)	1065, 1538
BtuMI	TCGCGA	1 cut(s)	1888
BveI	ACCTGC	1 cut(s)	860
Cac8I	GCNNGC	5 cut(s)	76, 399, 1219, 1890, 1955
CaiI	CAGNNNCTG	3 cut(s)	565, 914, 1334
CciI	TCATGA	1 cut(s)	361
CfoI	GCGC	2 cut(s)	252, 2155
Cfr10I	RCCGGY	3 cut(s)	74, 598, 755
Cfr13I	GGNCC	4 cut(s)	513, 652, 770, 779
Csp6I	GTAC	6 cut(s)	494, 525, 753, 1279, 1580, 2124
CspAI	ACCGGT	2 cut(s)	598, 755
CviQI	GTAC	6 cut(s)	494, 525, 753, 1279, 1580, 2124
DdeI	CTNAG	4 cut(s)	239, 1353, 1492, 2324
DpnI	GATC	6 cut(s)	43, 189, 197, 315, 1074, 1875
DpnII	GATC	6 cut(s)	41, 187, 195, 313, 1072, 1873
EaeI	YGGCCR	5 cut(s)	229, 253, 372, 879, 2196
EagI	CGGCCG	4 cut(s)	229, 253, 372, 879
Eam1104I	CTCTTC	3 cut(s)	157, 957, 2143
EarI	CTCTTC	3 cut(s)	157, 957, 2143
EciI	GGCGGA	1 cut(s)	183
Ecl136II	GAGCTC	1 cut(s)	1612
EclXI	CGGCCG	4 cut(s)	229, 253, 372, 879
Eco130I	CCWWGG	3 cut(s)	436, 2199, 2217
Eco147I	AGGCCT	1 cut(s)	949
Eco24I	GRGCYC	1 cut(s)	1614
Eco31I	GGTCTC	1 cut(s)	470
Eco47I	GGWCC	2 cut(s)	652, 770
Eco52I	CGGCCG	4 cut(s)	229, 253, 372, 879
Eco53kI	GAGCTC	1 cut(s)	1612
Eco57I	CTGAAG	4 cut(s)	795, 876, 1311, 1551
Eco88I	CYCGRG	1 cut(s)	2127
EcoICRI	GAGCTC	1 cut(s)	1612
EcoNI	CCTNNNNNAGG	1 cut(s)	2327
EcoO109I	RGGNCCY	2 cut(s)	770, 779
EcoRI	GAATTC	4 cut(s)	585, 1272, 1723, 2133
EcoRII	CCWGG	6 cut(s)	515, 575, 1267, 1333, 1501, 1689
EcoT14I	CCWWGG	3 cut(s)	436, 2199, 2217
EcoT22I	ATGCAT	4 cut(s)	633, 1955, 1959, 2068
EcoT38I	GRGCYC	1 cut(s)	1614
ErhI	CCWWGG	3 cut(s)	436, 2199, 2217
Esp3I	CGTCTC	1 cut(s)	242
FalI	AAGNNNNNCTT	2 cut(s)	1848, 1880
FaqI	GGGAC	1 cut(s)	1054
FauNDI	CATATG	1 cut(s)	2023
FbaI	TGATCA	1 cut(s)	195
FokI	GGATG	2 cut(s)	2105, 2234
FriOI	GRGCYC	1 cut(s)	1614
FspBI	CTAG	4 cut(s)	783, 1562, 2058, 2082
GlaI	GCGC	2 cut(s)	251, 2154
GsaI	CCCAGC	3 cut(s)	1226, 1248, 1525
GsuI	CTGGAG	2 cut(s)	31, 2027
HhaI	GCGC	2 cut(s)	252, 2155
Hin1I	GRCGYC	1 cut(s)	1589
Hin6I	GCGC	2 cut(s)	250, 2153
HinP1I	GCGC	2 cut(s)	250, 2153
HincII	GTYRAC	1 cut(s)	1350
HindII	GTYRAC	1 cut(s)	1350
HindIII	AAGCTT	1 cut(s)	1429
HpaI	GTTAAC	1 cut(s)	1350
HphI	GGTGA	4 cut(s)	308, 960, 1958, 2039
Hpy166II	GTNNAC	5 cut(s)	638, 1056, 1167, 1350, 1687
Hpy188I	TCNGA	9 cut(s)	153, 176, 345, 1714, 1837, 1843, 1935, 2255, 2327
Hpy188III	TCNNGA	8 cut(s)	113, 362, 773, 1868, 1887, 2006, 2177, 2314
Hpy8I	GTNNAC	5 cut(s)	638, 1056, 1167, 1350, 1687
Hpy99I	CGWCG	1 cut(s)	386
HpyAV	CCTTC	4 cut(s)	455, 530, 770, 1906
HpyCH4III	ACNGT	6 cut(s)	386, 940, 1021, 1060, 2040, 2353
HpyCH4IV	ACGT	1 cut(s)	1589
HpyF3I	CTNAG	4 cut(s)	239, 1353, 1492, 2324
HpySE526I	ACGT	1 cut(s)	1589
Hsp92I	GRCGYC	1 cut(s)	1589
HspAI	GCGC	2 cut(s)	250, 2153
Kpn2I	TCCGGA	1 cut(s)	2176
KpnI	GGTACC	1 cut(s)	756
KroI	GCCGGC	1 cut(s)	74
KroNI	GCCGGC	1 cut(s)	76
Ksp22I	TGATCA	1 cut(s)	195
KspAI	GTTAAC	1 cut(s)	1350
Kzo9I	GATC	6 cut(s)	41, 187, 195, 313, 1072, 1873
LguI	GCTCTTC	1 cut(s)	2143
LmnI	GCTCC	5 cut(s)	555, 1241, 1336, 1641, 2203
LweI	GCATC	7 cut(s)	346, 367, 589, 618, 2053, 2075, 2083
MaeI	CTAG	4 cut(s)	783, 1562, 2058, 2082
MaeII	ACGT	1 cut(s)	1589
MaeIII	GTNAC	3 cut(s)	601, 742, 1964
MalI	GATC	6 cut(s)	43, 189, 197, 315, 1074, 1875
MboI	GATC	6 cut(s)	41, 187, 195, 313, 1072, 1873
MflI	RGATCY	2 cut(s)	187, 1873
MhlI	GDGCHC	3 cut(s)	412, 1614, 1677
MlsI	TGGCCA	1 cut(s)	2198
MluNI	TGGCCA	1 cut(s)	2198
MlyI	GAGTC	8 cut(s)	22, 180, 374, 1036, 1141, 1832, 1924, 1972
MmeI	TCCRAC	4 cut(s)	154, 176, 1525, 1636
Mox20I	TGGCCA	1 cut(s)	2198
Mph1103I	ATGCAT	4 cut(s)	633, 1955, 1959, 2068
MreI	CGCCGGCG	1 cut(s)	74
MroI	TCCGGA	1 cut(s)	2176
MroNI	GCCGGC	1 cut(s)	74
MroXI	GAANNNNTTC	4 cut(s)	889, 1831, 1863, 2001
MscI	TGGCCA	1 cut(s)	2198
MseI	TTAA	8 cut(s)	122, 483, 824, 944, 1260, 1349, 2018, 2244
MslI	CAYNNNNRTG	2 cut(s)	418, 1794
Msp20I	TGGCCA	1 cut(s)	2198
MspA1I	CMGCKG	4 cut(s)	308, 740, 878, 1211
MspCI	CTTAAG	1 cut(s)	1259
MspR9I	CCNGG	6 cut(s)	517, 577, 1269, 1335, 1503, 1691
Mva1269I	GAATGC	2 cut(s)	1122, 1327
MvaI	CCWGG	6 cut(s)	517, 577, 1269, 1335, 1503, 1691
MvnI	CGCG	4 cut(s)	252, 905, 1888, 2155
NaeI	GCCGGC	1 cut(s)	76
NdeI	CATATG	1 cut(s)	2023
NdeII	GATC	6 cut(s)	41, 187, 195, 313, 1072, 1873
NgoMIV	GCCGGC	1 cut(s)	74
NlaIV	GGNNCC	5 cut(s)	551, 754, 771, 1674, 1917
NmeAIII	GCCGAG	1 cut(s)	281
NmuCI	GTSAC	3 cut(s)	601, 742, 1964
NruI	TCGCGA	1 cut(s)	1888
NsiI	ATGCAT	4 cut(s)	633, 1955, 1959, 2068
NspI	RCATGY	3 cut(s)	500, 1957, 2106
OliI	CACNNNNGTG	1 cut(s)	418
PaeI	GCATGC	1 cut(s)	1957
PaeR7I	CTCGAG	1 cut(s)	2127
PagI	TCATGA	1 cut(s)	361
PaqCI	CACCTGC	1 cut(s)	860
PceI	AGGCCT	1 cut(s)	949
PciI	ACATGT	2 cut(s)	496, 2102
PciSI	GCTCTTC	1 cut(s)	2143
PcsI	WCGNNNNNNNCGW	3 cut(s)	37, 390, 399
PctI	GAATGC	2 cut(s)	1122, 1327
PdiI	GCCGGC	1 cut(s)	76
PdmI	GAANNNNTTC	4 cut(s)	889, 1831, 1863, 2001
PfeI	GAWTC	8 cut(s)	346, 816, 1338, 1498, 2078, 2283, 2306, 2371
PfoI	TCCNGGA	3 cut(s)	1267, 1333, 1501
PinAI	ACCGGT	2 cut(s)	598, 755
PleI	GAGTC	8 cut(s)	22, 179, 373, 1036, 1141, 1832, 1924, 1971
PpsI	GAGTC	8 cut(s)	22, 179, 373, 1036, 1141, 1832, 1924, 1971
PpuMI	RGGWCCY	1 cut(s)	770
PscI	ACATGT	2 cut(s)	496, 2102
PshBI	ATTAAT	2 cut(s)	122, 824
Psp124BI	GAGCTC	1 cut(s)	1614
Psp5II	RGGWCCY	1 cut(s)	770
Psp6I	CCWGG	6 cut(s)	515, 575, 1267, 1333, 1501, 1689
PspFI	CCCAGC	3 cut(s)	1222, 1244, 1521
PspGI	CCWGG	6 cut(s)	515, 575, 1267, 1333, 1501, 1689
PspN4I	GGNNCC	5 cut(s)	551, 754, 771, 1674, 1917
PspPI	GGNCC	4 cut(s)	513, 652, 770, 779
PspPPI	RGGWCCY	1 cut(s)	770
PspXI	VCTCGAGB	1 cut(s)	2127
PsrI	GAACNNNNNNTAC	2 cut(s)	745, 777
PstI	CTGCAG	4 cut(s)	134, 561, 709, 1480
PstNI	CAGNNNCTG	3 cut(s)	565, 914, 1334
PsuI	RGATCY	2 cut(s)	187, 1873
PvuII	CAGCTG	2 cut(s)	740, 1211
RruI	TCGCGA	1 cut(s)	1888
RsaI	GTAC	6 cut(s)	495, 526, 754, 1280, 1581, 2125
RsaNI	GTAC	6 cut(s)	494, 525, 753, 1279, 1580, 2124
RseI	CAYNNNNRTG	2 cut(s)	418, 1794
SacI	GAGCTC	1 cut(s)	1614
SapI	GCTCTTC	1 cut(s)	2143
SaqAI	TTAA	8 cut(s)	122, 483, 824, 944, 1260, 1349, 2018, 2244
Sau3AI	GATC	6 cut(s)	41, 187, 195, 313, 1072, 1873
Sau96I	GGNCC	4 cut(s)	513, 652, 770, 779
SchI	GAGTC	8 cut(s)	22, 180, 374, 1036, 1141, 1832, 1924, 1972
ScrFI	CCNGG	6 cut(s)	517, 577, 1269, 1335, 1503, 1691
SduI	GDGCHC	3 cut(s)	412, 1614, 1677
SfaNI	GCATC	7 cut(s)	346, 367, 589, 618, 2053, 2075, 2083
SfcI	CTRYAG	6 cut(s)	130, 309, 557, 705, 1476, 2140
Sfr274I	CTCGAG	1 cut(s)	2127
SgrAI	CRCCGGYG	1 cut(s)	74
SinI	GGWCC	2 cut(s)	652, 770
SlaI	CTCGAG	1 cut(s)	2127
SmiMI	CAYNNNNRTG	2 cut(s)	418, 1794
SmlI	CTYRAG	3 cut(s)	111, 1259, 2127
SmoI	CTYRAG	3 cut(s)	111, 1259, 2127
SphI	GCATGC	1 cut(s)	1957
SseBI	AGGCCT	1 cut(s)	949
SsiI	CCGC	8 cut(s)	168, 232, 252, 279, 306, 477, 878, 905
SspI	AATATT	2 cut(s)	648, 2016
SspMI	CTAG	4 cut(s)	783, 1562, 2058, 2082
SstI	GAGCTC	1 cut(s)	1614
StuI	AGGCCT	1 cut(s)	949
StyD4I	CCNGG	6 cut(s)	515, 575, 1267, 1333, 1501, 1689
StyI	CCWWGG	3 cut(s)	436, 2199, 2217
TaaI	ACNGT	6 cut(s)	386, 940, 1021, 1060, 2040, 2353
TaiI	ACGT	1 cut(s)	1592
TaqI	TCGA	4 cut(s)	583, 955, 1150, 2128
TatI	WGTACW	2 cut(s)	1278, 2123
TauI	GCSGC	6 cut(s)	234, 255, 282, 479, 881, 908
TfiI	GAWTC	8 cut(s)	346, 816, 1338, 1498, 2078, 2283, 2306, 2371
Tru1I	TTAA	8 cut(s)	122, 483, 824, 944, 1260, 1349, 2018, 2244
Tru9I	TTAA	8 cut(s)	122, 483, 824, 944, 1260, 1349, 2018, 2244
TscAI	CASTG	2 cut(s)	1065, 1545
TseFI	GTSAC	3 cut(s)	601, 742, 1964
Tsp45I	GTSAC	3 cut(s)	601, 742, 1964
TspDTI	ATGAA	6 cut(s)	522, 1773, 1785, 2010, 2103, 2288
TspGWI	ACGGA	2 cut(s)	542, 1735
TspRI	CASTG	2 cut(s)	1065, 1545
Vha464I	CTTAAG	1 cut(s)	1259
VpaK11BI	GGWCC	2 cut(s)	652, 770
VspI	ATTAAT	2 cut(s)	122, 824
XagI	CCTNNNNNAGG	1 cut(s)	2327
XapI	RAATTY	6 cut(s)	585, 885, 1272, 1305, 1723, 2133
XceI	RCATGY	3 cut(s)	500, 1957, 2106
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2206
XhoI	CTCGAG	1 cut(s)	2127
XmnI	GAANNNNTTC	4 cut(s)	889, 1831, 1863, 2001
XspI	CTAG	4 cut(s)	783, 1562, 2058, 2082
ZraI	GACGTC	1 cut(s)	1590
Zsp2I	ATGCAT	4 cut(s)	633, 1955, 1959, 2068

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.