Rh5CG533600

Chloride channel protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0008013
Physical Location & Seq
Reverse (-)
74746153 .. 74752220
6068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG533600.1

Sequence Viewer

Length: 1779 bp
ATGATGCATGGTTTACTTGAAATATTGGATCAAATAAGACAGTTAAGTTCCTCCCAAGGGAAAGGATTTGATTTACTAGCTGGAGTCTTTCCCACAATAAAGGCTGTCCAGGCAGCTATCACACTAGGTACTGGTTGTTCTTTAGGTCCTGAAGGCCCTAGTGTAGATATTGGAAAATCATGTGCCAATGGGTTCTCATTAATGATGGAAAACAACAGAGAAAGGAAGATTGCCCTCATTGCAGCAGGTGCTGCTGCTGGAATTTCTTCAGGATTTAATGCGGCAGTTGCTGGTTGTTTCTTTGCTATTGAAACGGTGCTAAGGCCACTTCGAGCAGAAAACTCTCCTCCTTTTACTACCGCAATGATTATATTGGCTTCTGTTATATCGTCCACAGTATCTAATGTTTTACTTGGGACGCAATCAGCCTTTACAGTGCCTGTTTACGATTTGAAATCAGCTGCTGAATTGCCCCTCTACCTTATATTGGGCATGCTATGTGGTGCTGTGAGTGTAGTCTTCAATCGCTTGGTTGCTTGGTTCACAAAGTTTTTTGATTATATCAAGGAAAGATTTGGCCTTCCTGCAGTTGCATGCCCTGCTTTAGGTGGTTTAGGAGCTGGGTTGATTGCTCTCAAGTATCCAGGAATTCTGTACTGGGGTTTCACAAATGTTGAAGAAATCCTGCATACTGGAAAGACTGCTTCCGCCCCTGGAATCTGGCTGTTAACTCAATTATCAGCAGCCAAAGTTGTAGCAACAGCTTTATGCAAGGGTTCTGGGCTTGTAGGTGGACTTTATGCACCAAGCTTGATGATTGGGGCTGCTGTTGGCGCTGTATTTGGGGGTTCAGCTGCAGAATTGATTAATTCAGCAATTCCAGGAAATGCTGCCGTTGCTCAGCCACAGGCATATGCACTGGTGGGAATGGCTGCTATGCTGGCTTCAGTTTGTTCGGTGCCATTGACGTCAGTTCTCCTTCTGTTTGAGCTGACAAAAGATTACCGAATATTGCTTCCTCTCATGGGGGCGGTTGGATTGGCAATTTGGGTTCCCTCTGTGGTAAACCAGGGAAAGGAGACTGAAGCATCCGATACACGGAATTCTGCGAGAGGTTACTCTTCTGTTTCAGCTGCTGAAGAGAAAGATGAAGTTATTTGGAGACAACATGATAATGGAGATGATATGGAACTGTCTACCATGGGGAATACTTCTGATTCTGAATTAACTGAAGAAATGCTTCTGGAGAATCTAAAGGTTTCGCGGGCCATGTCAAAGAATTATGTGAAGGTTTCTCTGACCGTAACTATTGAAGAGGCAATAAAATCCATGCATGACAAGCATCAGAATTGTGTGTTAATTGTTGATGATGAAGATTTTCTGGAGGGAATATTGACATATGGTGATGTTAGACGGTATCAATCAAGGAAGTCTTCTGATACGTTGAAGATCGATTCTAGTTTTCTGGATGAAAACACCTGTCTTGTTTCCGCTGTATGTACTCGAGAAATCAGCTTTCATGGGCGAACCCGAGGTCTATTAACCTGTTATCCGGACACGGGTTTATTAATGGCCAAGGAGCTAATGGAGGCAAAAGATATCAAGCAGTTGCCTGTGGTTAAGCGTGGCAGACAACCACCAAAAGAAACGAGGCGAAGACTTGTTGCTATTCTTCATTACGATTCAATCTTGATGTGTCTCAGGGAGGAGATAAATCACAGGAAATCAATTCATCAACATAGAAAAGAGAATCTTGACGAAATAACAAATGGCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0009507 GO:0009536 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBS Voltage_CLC

Protein Analysis

592

Amino Acids

63.64

Weight (kDa)

6.17

Isoelectric Point (pI)

38.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Voltage_CLC PF00654 27 - 351 8.8e-72 Voltage gated chloride channel
CBS PF00571 420 - 474 9.7e-09 CBS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 236
AatII GACGTC 1 cut(s) 971
Acc36I ACCTGC 1 cut(s) 236
AccB1I GGYRCC 1 cut(s) 958
AccI GTMKAC 1 cut(s) 1196
AccII CGCG 1 cut(s) 1264
AccIII TCCGGA 1 cut(s) 1552
AciI CCGC 6 cut(s) 281, 360, 708, 1031, 1264, 1491
AclWI GGATC 1 cut(s) 36
AcoI YGGCCR 2 cut(s) 1572, 1771
AcsI RAATTY 3 cut(s) 261, 648, 1102
AcuI CTGAAG 6 cut(s) 171, 252, 930, 1104, 1158, 1251
AcyI GRCGYC 1 cut(s) 968
AfaI GTAC 3 cut(s) 130, 656, 1501
AfiI CCNNNNNNNGG 7 cut(s) 57, 487, 605, 1025, 1075, 1098, 1559
AgsI TTSAA 8 cut(s) 20, 311, 454, 523, 677, 1313, 1447, 1686
AjnI CCWGG 5 cut(s) 108, 643, 712, 880, 1068
Alw26I GTCTC 3 cut(s) 1073, 1156, 1703
AlwI GGATC 1 cut(s) 36
AlwNI CAGNNNCTG 5 cut(s) 251, 290, 440, 464, 1136
Ama87I CYCGRG 2 cut(s) 1503, 1530
Aor13HI TCCGGA 1 cut(s) 1552
AoxI GGCC 6 cut(s) 154, 323, 577, 1266, 1572, 1771
ApoI RAATTY 3 cut(s) 261, 648, 1102
ArsI GACNNNNNNTTYG 2 cut(s) 1519, 1551
AseI ATTAAT 3 cut(s) 200, 867, 1568
Asp700I GAANNNNTTC 3 cut(s) 265, 1239, 1377
AspLEI GCGC 1 cut(s) 836
AspS9I GGNCC 3 cut(s) 146, 155, 1266
AsuHPI GGTGA 1 cut(s) 1415
AvaI CYCGRG 2 cut(s) 1503, 1530
AvaII GGWCC 1 cut(s) 146
BalI TGGCCA 2 cut(s) 1574, 1773
BanI GGYRCC 1 cut(s) 958
BbsI GAAGAC 3 cut(s) 511, 1425, 1663
BccI CCATC 1 cut(s) 199
BceAI ACGGC 1 cut(s) 878
BciT130I CCWGG 5 cut(s) 110, 645, 714, 882, 1070
BciVI GTATCC 1 cut(s) 651
BcoDI GTCTC 3 cut(s) 1073, 1156, 1703
BfaI CTAG 4 cut(s) 77, 125, 159, 1458
BfmI CTRYAG 2 cut(s) 585, 855
BfoI RGCGCY 1 cut(s) 837
BfuAI ACCTGC 1 cut(s) 236
BfuI GTATCC 1 cut(s) 651
BlpI GCTNAGC 1 cut(s) 900
Bme1390I CCNGG 5 cut(s) 110, 645, 714, 882, 1070
Bme18I GGWCC 1 cut(s) 146
BmeT110I CYCGRG 2 cut(s) 1503, 1530
BmgT120I GGNCC 3 cut(s) 146, 155, 1266
BmiI GGNNCC 2 cut(s) 960, 1053
BmrFI CCNGG 5 cut(s) 110, 645, 714, 882, 1070
BmrI ACTGGG 1 cut(s) 667
BmsI GCATC 2 cut(s) 1097, 1351
BmuI ACTGGG 1 cut(s) 667
BpiI GAAGAC 3 cut(s) 511, 1425, 1663
BpmI CTGGAG 3 cut(s) 102, 1265, 1403
Bpu10I CCTNAGC 1 cut(s) 320
Bpu1102I GCTNAGC 1 cut(s) 900
BpuEI CTTGAG 1 cut(s) 620
Bsa29I ATCGAT 1 cut(s) 1452
BsaHI GRCGYC 1 cut(s) 968
BsaJI CCNNGG 6 cut(s) 55, 712, 1069, 1200, 1531, 1575
BsaWI WCCGGW 1 cut(s) 1552
Bsc4I CCNNNNNNNGG 7 cut(s) 57, 487, 605, 1025, 1075, 1098, 1559
Bse1I ACTGG 4 cut(s) 136, 662, 697, 924
Bse3DI GCAATG 2 cut(s) 237, 369
BseAI TCCGGA 1 cut(s) 1552
BseBI CCWGG 5 cut(s) 110, 645, 714, 882, 1070
BseCI ATCGAT 1 cut(s) 1452
BseDI CCNNGG 6 cut(s) 55, 712, 1069, 1200, 1531, 1575
BseGI GGATG 2 cut(s) 1088, 1474
BseLI CCNNNNNNNGG 7 cut(s) 57, 487, 605, 1025, 1075, 1098, 1559
BseMI GCAATG 2 cut(s) 237, 369
BseMII CTCAG 2 cut(s) 914, 1714
BseNI ACTGG 4 cut(s) 136, 662, 697, 924
BseRI GAGGAG 2 cut(s) 336, 1721
BseYI CCCAGC 1 cut(s) 620
Bsh1236I CGCG 1 cut(s) 1264
BshFI GGCC 6 cut(s) 156, 325, 579, 1268, 1574, 1773
BshNI GGYRCC 1 cut(s) 958
BshVI ATCGAT 1 cut(s) 1452
BsiHKCI CYCGRG 2 cut(s) 1503, 1530
BsiSI CCGG 1 cut(s) 1553
BslFI GGGAC 1 cut(s) 430
BslI CCNNNNNNNGG 7 cut(s) 57, 487, 605, 1025, 1075, 1098, 1559
BsmAI GTCTC 3 cut(s) 1073, 1156, 1703
BsmFI GGGAC 1 cut(s) 430
BsnI GGCC 6 cut(s) 156, 325, 579, 1268, 1574, 1773
BsoBI CYCGRG 2 cut(s) 1503, 1530
Bsp13I TCCGGA 1 cut(s) 1552
Bsp143I GATC 2 cut(s) 28, 1449
Bsp1720I GCTNAGC 1 cut(s) 900
Bsp19I CCATGG 1 cut(s) 1200
BspACI CCGC 6 cut(s) 281, 360, 708, 1031, 1264, 1491
BspANI GGCC 6 cut(s) 156, 325, 579, 1268, 1574, 1773
BspCNI CTCAG 2 cut(s) 913, 1713
BspDI ATCGAT 1 cut(s) 1452
BspEI TCCGGA 1 cut(s) 1552
BspFNI CGCG 1 cut(s) 1264
BspLI GGNNCC 2 cut(s) 960, 1053
BspMAI CTGCAG 2 cut(s) 589, 859
BspMI ACCTGC 1 cut(s) 236
BspPI GGATC 1 cut(s) 36
BspT107I GGYRCC 1 cut(s) 958
BsrDI GCAATG 2 cut(s) 237, 369
BsrI ACTGG 4 cut(s) 136, 662, 697, 924
BssECI CCNNGG 6 cut(s) 55, 712, 1069, 1200, 1531, 1575
BssMI GATC 2 cut(s) 28, 1449
BssNI GRCGYC 1 cut(s) 968
BssT1I CCWWGG 3 cut(s) 55, 1200, 1575
Bst2UI CCWGG 5 cut(s) 110, 645, 714, 882, 1070
Bst4CI ACNGT 7 cut(s) 42, 316, 397, 436, 1194, 1303, 1416
Bst6I CTCTTC 3 cut(s) 1126, 1134, 1308
BstACI GRCGYC 1 cut(s) 968
BstAPI GCANNNNNTGC 3 cut(s) 248, 251, 599
BstC8I GCNNGC 4 cut(s) 494, 595, 942, 1266
BstDEI CTNAG 3 cut(s) 320, 900, 1700
BstDSI CCRYGG 1 cut(s) 1200
BstENI CCTNNNNNAGG 1 cut(s) 603
BstF5I GGATG 2 cut(s) 1088, 1474
BstFNI CGCG 1 cut(s) 1264
BstH2I RGCGCY 1 cut(s) 837
BstHHI GCGC 1 cut(s) 836
BstKTI GATC 2 cut(s) 31, 1452
BstMAI GTCTC 3 cut(s) 1073, 1156, 1703
BstMBI GATC 2 cut(s) 28, 1449
BstNI CCWGG 5 cut(s) 110, 645, 714, 882, 1070
BstNSI RCATGY 2 cut(s) 496, 597
BstSCI CCNGG 5 cut(s) 108, 643, 712, 880, 1068
BstSFI CTRYAG 2 cut(s) 585, 855
BstUI CGCG 1 cut(s) 1264
BstV2I GAAGAC 3 cut(s) 511, 1425, 1663
Bsu15I ATCGAT 1 cut(s) 1452
BsuI GTATCC 1 cut(s) 651
BsuRI GGCC 6 cut(s) 156, 325, 579, 1268, 1574, 1773
BsuTUI ATCGAT 1 cut(s) 1452
BtgI CCRYGG 1 cut(s) 1200
BtsCI GGATG 2 cut(s) 1088, 1474
BtsIMutI CAGTG 2 cut(s) 441, 917
BveI ACCTGC 1 cut(s) 236
Cac8I GCNNGC 4 cut(s) 494, 595, 942, 1266
CaiI CAGNNNCTG 5 cut(s) 251, 290, 440, 464, 1136
CfoI GCGC 1 cut(s) 836
Cfr13I GGNCC 3 cut(s) 146, 155, 1266
ClaI ATCGAT 1 cut(s) 1452
CseI GACGC 1 cut(s) 427
Csp6I GTAC 3 cut(s) 129, 655, 1500
CviQI GTAC 3 cut(s) 129, 655, 1500
DdeI CTNAG 3 cut(s) 320, 900, 1700
DpnI GATC 2 cut(s) 30, 1451
DpnII GATC 2 cut(s) 28, 1449
EaeI YGGCCR 2 cut(s) 1572, 1771
Eam1104I CTCTTC 3 cut(s) 1126, 1134, 1308
EarI CTCTTC 3 cut(s) 1126, 1134, 1308
EciI GGCGGA 1 cut(s) 697
Eco130I CCWWGG 3 cut(s) 55, 1200, 1575
Eco32I GATATC 1 cut(s) 1600
Eco47I GGWCC 1 cut(s) 146
Eco57I CTGAAG 6 cut(s) 171, 252, 930, 1104, 1158, 1251
Eco88I CYCGRG 2 cut(s) 1503, 1530
EcoNI CCTNNNNNAGG 1 cut(s) 603
EcoO109I RGGNCCY 2 cut(s) 146, 155
EcoRI GAATTC 2 cut(s) 648, 1102
EcoRII CCWGG 5 cut(s) 108, 643, 712, 880, 1068
EcoRV GATATC 1 cut(s) 1600
EcoT14I CCWWGG 3 cut(s) 55, 1200, 1575
EcoT22I ATGCAT 2 cut(s) 9, 1335
ErhI CCWWGG 3 cut(s) 55, 1200, 1575
FalI AAGNNNNNCTT 6 cut(s) 1224, 1256, 1417, 1449, 1737, 1769
FaqI GGGAC 1 cut(s) 430
FauI CCCGC 1 cut(s) 1257
FauNDI CATATG 2 cut(s) 913, 1399
FblI GTMKAC 1 cut(s) 1196
FokI GGATG 2 cut(s) 1075, 1481
FspBI CTAG 4 cut(s) 77, 125, 159, 1458
GlaI GCGC 1 cut(s) 835
GsaI CCCAGC 1 cut(s) 624
GsuI CTGGAG 3 cut(s) 102, 1265, 1403
HaeII RGCGCY 1 cut(s) 837
HaeIII GGCC 6 cut(s) 156, 325, 579, 1268, 1574, 1773
HapII CCGG 1 cut(s) 1553
HgaI GACGC 1 cut(s) 427
HhaI GCGC 1 cut(s) 836
Hin1I GRCGYC 1 cut(s) 968
Hin6I GCGC 1 cut(s) 834
HinP1I GCGC 1 cut(s) 834
HincII GTYRAC 1 cut(s) 729
HindII GTYRAC 1 cut(s) 729
HindIII AAGCTT 1 cut(s) 808
HinfI GANTC 7 cut(s) 84, 717, 1217, 1249, 1454, 1682, 1750
HpaI GTTAAC 1 cut(s) 729
HpaII CCGG 1 cut(s) 1553
HphI GGTGA 1 cut(s) 1415
Hpy166II GTNNAC 8 cut(s) 14, 393, 445, 543, 729, 794, 1066, 1197
Hpy188I TCNGA 6 cut(s) 1093, 1216, 1222, 1299, 1347, 1438
Hpy188III TCNNGA 9 cut(s) 149, 270, 1244, 1382, 1466, 1505, 1553, 1690, 1754
Hpy8I GTNNAC 8 cut(s) 14, 393, 445, 543, 729, 794, 1066, 1197
HpyAV CCTTC 4 cut(s) 146, 590, 989, 1282
HpyCH4III ACNGT 7 cut(s) 42, 316, 397, 436, 1194, 1303, 1416
HpyCH4IV ACGT 2 cut(s) 968, 1442
HpyF3I CTNAG 3 cut(s) 320, 900, 1700
HpySE526I ACGT 2 cut(s) 968, 1442
Hsp92I GRCGYC 1 cut(s) 968
HspAI GCGC 1 cut(s) 834
Kpn2I TCCGGA 1 cut(s) 1552
KspAI GTTAAC 1 cut(s) 729
Kzo9I GATC 2 cut(s) 28, 1449
LmnI GCTCC 2 cut(s) 617, 1579
LweI GCATC 2 cut(s) 1097, 1351
MaeI CTAG 4 cut(s) 77, 125, 159, 1458
MaeII ACGT 2 cut(s) 968, 1442
MaeIII GTNAC 2 cut(s) 1115, 1303
MalI GATC 2 cut(s) 30, 1451
MboI GATC 2 cut(s) 28, 1449
MlsI TGGCCA 2 cut(s) 1574, 1773
MluNI TGGCCA 2 cut(s) 1574, 1773
MlyI GAGTC 1 cut(s) 93
MmeI TCCRAC 1 cut(s) 1015
Mox20I TGGCCA 2 cut(s) 1574, 1773
Mph1103I ATGCAT 2 cut(s) 9, 1335
MroI TCCGGA 1 cut(s) 1552
MroXI GAANNNNTTC 3 cut(s) 265, 1239, 1377
MscI TGGCCA 2 cut(s) 1574, 1773
MslI CAYNNNNRTG 1 cut(s) 1173
Msp20I TGGCCA 2 cut(s) 1574, 1773
MspA1I CMGCKG 4 cut(s) 461, 854, 1133, 1493
MspI CCGG 1 cut(s) 1553
MspR9I CCNGG 5 cut(s) 110, 645, 714, 882, 1070
MvaI CCWGG 5 cut(s) 110, 645, 714, 882, 1070
MvnI CGCG 1 cut(s) 1264
NcoI CCATGG 1 cut(s) 1200
NdeI CATATG 2 cut(s) 913, 1399
NdeII GATC 2 cut(s) 28, 1449
NlaIV GGNNCC 2 cut(s) 960, 1053
NsiI ATGCAT 2 cut(s) 9, 1335
NspI RCATGY 2 cut(s) 496, 597
PaeI GCATGC 2 cut(s) 496, 597
PaeR7I CTCGAG 1 cut(s) 1503
PaqCI CACCTGC 1 cut(s) 236
PdmI GAANNNNTTC 3 cut(s) 265, 1239, 1377
PfeI GAWTC 6 cut(s) 717, 1217, 1249, 1454, 1682, 1750
PfoI TCCNGGA 2 cut(s) 643, 880
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
PpuMI RGGWCCY 1 cut(s) 146
PshBI ATTAAT 3 cut(s) 200, 867, 1568
Psp5II RGGWCCY 1 cut(s) 146
Psp6I CCWGG 5 cut(s) 108, 643, 712, 880, 1068
PspFI CCCAGC 1 cut(s) 620
PspGI CCWGG 5 cut(s) 108, 643, 712, 880, 1068
PspN4I GGNNCC 2 cut(s) 960, 1053
PspPI GGNCC 3 cut(s) 146, 155, 1266
PspPPI RGGWCCY 1 cut(s) 146
PsrI GAACNNNNNNTAC 2 cut(s) 121, 153
PstI CTGCAG 2 cut(s) 589, 859
PstNI CAGNNNCTG 5 cut(s) 251, 290, 440, 464, 1136
PvuII CAGCTG 3 cut(s) 461, 854, 1133
RsaI GTAC 3 cut(s) 130, 656, 1501
RsaNI GTAC 3 cut(s) 129, 655, 1500
RseI CAYNNNNRTG 1 cut(s) 1173
Sau3AI GATC 2 cut(s) 28, 1449
Sau96I GGNCC 3 cut(s) 146, 155, 1266
SchI GAGTC 1 cut(s) 93
ScrFI CCNGG 5 cut(s) 110, 645, 714, 882, 1070
SfaNI GCATC 2 cut(s) 1097, 1351
SfcI CTRYAG 2 cut(s) 585, 855
Sfr274I CTCGAG 1 cut(s) 1503
SinI GGWCC 1 cut(s) 146
SlaI CTCGAG 1 cut(s) 1503
SmiMI CAYNNNNRTG 1 cut(s) 1173
SmlI CTYRAG 2 cut(s) 635, 1503
SmoI CTYRAG 2 cut(s) 635, 1503
SphI GCATGC 2 cut(s) 496, 597
SsiI CCGC 6 cut(s) 281, 360, 708, 1031, 1264, 1491
SspI AATATT 3 cut(s) 24, 1011, 1392
SspMI CTAG 4 cut(s) 77, 125, 159, 1458
StyD4I CCNGG 5 cut(s) 108, 643, 712, 880, 1068
StyI CCWWGG 3 cut(s) 55, 1200, 1575
TaaI ACNGT 7 cut(s) 42, 316, 397, 436, 1194, 1303, 1416
TaiI ACGT 2 cut(s) 971, 1445
TaqI TCGA 3 cut(s) 331, 1452, 1504
TatI WGTACW 2 cut(s) 654, 1499
TauI GCSGC 1 cut(s) 284
TfiI GAWTC 6 cut(s) 717, 1217, 1249, 1454, 1682, 1750
TscAI CASTG 2 cut(s) 441, 924
TspDTI ATGAA 6 cut(s) 1164, 1386, 1485, 1508, 1664, 1721
TspGWI ACGGA 1 cut(s) 1114
TspRI CASTG 2 cut(s) 441, 924
VpaK11BI GGWCC 1 cut(s) 146
VspI ATTAAT 3 cut(s) 200, 867, 1568
XagI CCTNNNNNAGG 1 cut(s) 603
XapI RAATTY 3 cut(s) 261, 648, 1102
XceI RCATGY 2 cut(s) 496, 597
XcmI CCANNNNNNNNNTGG 1 cut(s) 1582
XhoI CTCGAG 1 cut(s) 1503
XmiI GTMKAC 1 cut(s) 1196
XmnI GAANNNNTTC 3 cut(s) 265, 1239, 1377
XspI CTAG 4 cut(s) 77, 125, 159, 1458
ZraI GACGTC 1 cut(s) 969
Zsp2I ATGCAT 2 cut(s) 9, 1335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.