pycom11g03830

Chloride channel protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0008013
Physical Location & Seq
Forward (+)
2981797 .. 2987116
5320 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g03830.1

Sequence Viewer

Length: 2391 bp
ATGTCAGGAGAGGAATACAGCGATGAGAATCATCTGCTGCGATCCTCCAACGACGTTACCAACGACGAAAACGCCGGCGTTTCGGCAGAGAAAGACAACGACTTGGAAGCTCAGGAGGGATTAATAAGTAGCAGGAAAAGCAGTATTAGTCCTACCAGTAGACGAGGCGGAGTCGGAGGCTTCAAGGATCTGCTGATCAAGCATTTGGACGGCGGAGGTGGGCTTTCCGGCCGCCGTCTCAATGTCAAGCGCGGCCGAGATAATTCCCCAAATCATGGCGGTAGAGAGATTAGAGACCAGCAACGGCCCCCTGTAGATCACCACCACCATCATAATCAGCAGCATCATGAGTCTTCGGCCGGAATAGACGGTAATGATGACCTTGCCGATAGTGCGCCGCCCGAATGGGCTTTGCTTCTCCTTGGTTGCCTTCTCGGCCTCGCCACTGGTCTCCTTGTTGCCGCATTTAACAAAGGGGTACATGTTATACATGAATGGGCCTGGGCTGGTACTCCAAGTGATGGTGCTGCATGGCTTCGTCTGCAGAGACTGGGCGATACTTGGCATCGAATTCTCTTAATACCAGTCACGGGTGGGGTTATTGTTGGCATGATGCATGGTTTACTCGAAATATTAGGCCAGATAAGGCAGTCCGGTTCCTCTCAAAGACAAGGATTTGATTTACTTGCTGCAGTCTTTCCCTCAATAAAGGCTGTCCAAGCAGCTGTCACTTTAGGTACTGGTTGTTCTTTGGGTCCTGAAGGCCCTAGTGTAGATATTGGAAAATCTTGTGCCAATGGAATCTCATTAATGATGGAGAACAACAGAGAAAGGAAGATTGCCCTTGTTGCAGCAGGTGCAGCAGCTGGAATTTCTTCAGGTTTCAACGCGGCAGTTGCTGGTTGTTTCTTCGCTATTGAAACGGTGTTAAGGCCTCTTCGAGCAGAAAACTCACCTCCATTTACAACTGCAATGATTATTTTGGCTTCTGTTATATCATCCACTGTATCTAATGTTTTACAAGGGACTCAGTCAGCATTTACAGTGCCTACATATGATTTAAAATCTGCTGCTGAACTTCCTCTATACCTGATATTGGGAATGCTATGTGGTGCTGTAAGTGTAGCCTTGACTCGATTGGTTGCTTGGTTCACAAAGTTCTTTGATTTTATCAAGGAAAGATTTGGTCTTCCTGATGTTGCCTGCCCAGCTTTAGGTGGTTTAGGAGCTGGGATTATCGCTCTTAAGTATCCTGGAATTCTGTATTGGGGATTTACAAATGTTGAAGAAATTTTGCACACTGGGAGGACTGCTTCAGCTCCTGGAATCTGGCTGTTAACTCAGTTATCAGCAGCTAAAGTTGTGGCAACAGCTTTATGCAAGGGTTCTGGGCTGGTAGGTGGCCTATACGCACCAAGCTTGATGATTGGTGCTGCTGTTGGTGCTGTATTTGGGGGTTCAGCTGCAGAACTGATAAACTCAGCAATTCCAGGAAATGCTGCTGTTGCCCAGCCACAGGCTTATGCACTGGTTGGAATGGCTGCTACGCTAGCTTCAGTTTGTTCAGTACCATTGACGTCAGTTCTGCTTCTGTTTGAGCTCACAAAAGACTACCGGATATTGCTCCCTCTCATGGGGGCTGTTGGATTGGCAATATGGGTGCCCACTGTGGTAAACCAGGCAAAGGAGACTGAACCATCTGAAACACAGAATTCAGCAAGAGTTTATTCTTACGTTTCAGCTGATGAAGAAAAAGACGAAGTTAGTTGGAGACAACGTGATGATGGAGATGACTTGGAACTCACTGTTATTGGAAACAATTCTGACTCCGAATTAGTTATTGAAGAATTGCTTCTGGAAGATCTAAAGGTTTCTCAAGCCATGTCAAAGAAATATGTGAAGGTTCCTGTGAGCTTGACTCTGAAAGAGGCAATAAAATGCATGCACGAGAGTCATCAGAATTGTGTGTTGGTGGTTGATGATGAAGATTTTCTGGAGGGAGTATTAACATACGGTGATGTTAGACGATTTCAATCGAAGAATTCTAGTGATACATCAAAGAGCGATACTAGATTTTTGGATGCAAATACATGTCTTGTTTCATCTGTTTGCACTCGACGAGCTCTCTATAGTGGACGAGCGCGTGGTTTATTTACCTGTTATCCGGACACAGATTTAGCAATGGCCAAGGAGCTAATGGAGGCCAAGGATATAAGGCAGTTGCCTGTGGTTAAGCGTGTCAGAGAACCACCAAAAGAAATAAAGCGCAGAATCGTTGCTATTCTTCATTATGATTCAATCTTGAACTGTCTCAGAGAGGAGATAAATCACAGGAAAACAGTTCATCAACATAGAATAGAGAATCTCGACGAGATTAACAATGGCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0009507 GO:0009536 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBS Voltage_CLC

Protein Analysis

797

Amino Acids

85.59

Weight (kDa)

5.9

Isoelectric Point (pI)

42.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Voltage_CLC PF00654 230 - 554 6e-68 Voltage gated chloride channel
CBS PF00571 624 - 678 7e-09 CBS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 845
AatII GACGTC 1 cut(s) 1580
Acc36I ACCTGC 1 cut(s) 845
AccB1I GGYRCC 1 cut(s) 1660
AccB7I CCANNNNNTGG 2 cut(s) 275, 521
AccI GTMKAC 1 cut(s) 160
AccII CGCG 3 cut(s) 252, 890, 2143
AccIII TCCGGA 1 cut(s) 2164
AciI CCGC 8 cut(s) 168, 213, 232, 252, 279, 398, 462, 890
AclWI GGATC 2 cut(s) 36, 195
AcoI YGGCCR 5 cut(s) 229, 253, 357, 2184, 2383
AcsI RAATTY 6 cut(s) 570, 870, 1257, 1290, 1711, 2041
AcuI CTGAAG 4 cut(s) 780, 861, 1299, 1539
AcyI GRCGYC 1 cut(s) 1577
AfaI GTAC 4 cut(s) 480, 511, 739, 1569
AfiI CCNNNNNNNGG 9 cut(s) 275, 521, 590, 1096, 1214, 1516, 1633, 1634, 1684
AflII CTTAAG 1 cut(s) 1244
AflIII ACRYGT 2 cut(s) 481, 2090
AgsI TTSAA 8 cut(s) 184, 886, 920, 1286, 1844, 2033, 2298, 2305
AjnI CCWGG 5 cut(s) 500, 1252, 1321, 1489, 1677
AjuI GAANNNNNNNTTGG 4 cut(s) 1249, 1281, 1952, 1984
Alw21I GWGCWC 2 cut(s) 1602, 2125
Alw26I GTCTC 7 cut(s) 242, 288, 455, 541, 1682, 1765, 2315
AlwI GGATC 2 cut(s) 36, 195
AlwNI CAGNNNCTG 4 cut(s) 550, 866, 899, 1322
Aor13HI TCCGGA 1 cut(s) 2164
ApoI RAATTY 6 cut(s) 570, 870, 1257, 1290, 1711, 2041
AseI ATTAAT 2 cut(s) 122, 809
Asp700I GAANNNNTTC 4 cut(s) 874, 1819, 1851, 1989
AspLEI GCGC 4 cut(s) 252, 397, 2143, 2268
AspS9I GGNCC 4 cut(s) 306, 498, 755, 764
AsuHPI GGTGA 3 cut(s) 311, 945, 2027
AsuNHI GCTAGC 1 cut(s) 1549
AvaII GGWCC 1 cut(s) 755
BaeGI GKGCMC 1 cut(s) 1665
BalI TGGCCA 2 cut(s) 2186, 2385
BanI GGYRCC 1 cut(s) 1660
BanII GRGCYC 2 cut(s) 1602, 2125
BauI CACGAG 1 cut(s) 1946
BbsI GAAGAC 2 cut(s) 345, 1181
Bbv12I GWGCWC 2 cut(s) 1602, 2125
BccI CCATC 5 cut(s) 336, 515, 808, 1705, 1778
BceAI ACGGC 3 cut(s) 219, 226, 320
BciT130I CCWGG 5 cut(s) 502, 1254, 1323, 1491, 1679
BciVI GTATCC 1 cut(s) 1260
BclI TGATCA 1 cut(s) 195
BcoDI GTCTC 7 cut(s) 242, 288, 455, 541, 1682, 1765, 2315
BfaI CTAG 4 cut(s) 768, 1550, 2046, 2070
BfmI CTRYAG 5 cut(s) 312, 542, 690, 1464, 2128
BfrI CTTAAG 1 cut(s) 1244
BfuAI ACCTGC 1 cut(s) 845
BfuI GTATCC 1 cut(s) 1260
BglI GCCNNNNNGGC 1 cut(s) 435
BglII AGATCT 1 cut(s) 1861
Bme1390I CCNGG 5 cut(s) 502, 1254, 1323, 1491, 1679
Bme18I GGWCC 1 cut(s) 755
BmgT120I GGNCC 4 cut(s) 306, 498, 755, 764
BmiI GGNNCC 5 cut(s) 308, 658, 756, 1662, 1905
BmrFI CCNGG 5 cut(s) 502, 1254, 1323, 1491, 1679
BmrI ACTGGG 2 cut(s) 560, 1311
BmsI GCATC 4 cut(s) 352, 574, 603, 2071
BmtI GCTAGC 1 cut(s) 1553
BmuI ACTGGG 2 cut(s) 560, 1311
BpiI GAAGAC 2 cut(s) 345, 1181
BplI GAGNNNNNCTC 2 cut(s) 1903, 1935
BpmI CTGGAG 1 cut(s) 2015
Bpu10I CCTNAGC 1 cut(s) 111
BpuEI CTTGAG 1 cut(s) 1860
BsaBI GATNNNNATC 2 cut(s) 27, 2032
BsaHI GRCGYC 1 cut(s) 1577
BsaI GGTCTC 2 cut(s) 288, 455
BsaJI CCNNGG 4 cut(s) 421, 501, 2187, 2205
BsaWI WCCGGW 3 cut(s) 653, 1614, 2164
BsaXI ACNNNNNCTCC 2 cut(s) 1762, 1792
Bsc4I CCNNNNNNNGG 9 cut(s) 275, 521, 590, 1096, 1214, 1516, 1633, 1634, 1684
Bse118I RCCGGY 1 cut(s) 74
Bse1I ACTGG 7 cut(s) 156, 451, 555, 584, 745, 1306, 1533
Bse3DI GCAATG 2 cut(s) 978, 2187
Bse8I GATNNNNATC 2 cut(s) 27, 2032
BseAI TCCGGA 1 cut(s) 2164
BseBI CCWGG 5 cut(s) 502, 1254, 1323, 1491, 1679
BseDI CCNNGG 4 cut(s) 421, 501, 2187, 2205
BseGI GGATG 2 cut(s) 998, 2086
BseJI GATNNNNATC 2 cut(s) 27, 2032
BseLI CCNNNNNNNGG 9 cut(s) 275, 521, 590, 1096, 1214, 1516, 1633, 1634, 1684
BseMI GCAATG 2 cut(s) 978, 2187
BseMII CTCAG 5 cut(s) 125, 1043, 1355, 1494, 2326
BseNI ACTGG 7 cut(s) 156, 451, 555, 584, 745, 1306, 1533
BseRI GAGGAG 1 cut(s) 2333
BseSI GKGCMC 1 cut(s) 1665
BseX3I CGGCCG 3 cut(s) 229, 253, 357
BseYI CCCAGC 3 cut(s) 1207, 1229, 1509
BsgI GTGCAG 1 cut(s) 879
Bsh1236I CGCG 3 cut(s) 252, 890, 2143
Bsh1285I CGRYCG 3 cut(s) 232, 256, 360
BshNI GGYRCC 1 cut(s) 1660
BsiEI CGRYCG 3 cut(s) 232, 256, 360
BsiHKAI GWGCWC 2 cut(s) 1602, 2125
BsiSI CCGG 6 cut(s) 75, 228, 360, 654, 1615, 2165
BslFI GGGAC 1 cut(s) 1039
BslI CCNNNNNNNGG 9 cut(s) 275, 521, 590, 1096, 1214, 1516, 1633, 1634, 1684
BsmAI GTCTC 7 cut(s) 242, 288, 455, 541, 1682, 1765, 2315
BsmBI CGTCTC 1 cut(s) 242
BsmFI GGGAC 1 cut(s) 1039
BsmI GAATGC 1 cut(s) 1107
Bso31I GGTCTC 2 cut(s) 288, 455
Bsp1286I GDGCHC 3 cut(s) 1602, 1665, 2125
Bsp13I TCCGGA 1 cut(s) 2164
Bsp143I GATC 5 cut(s) 41, 187, 195, 316, 1861
BspACI CCGC 8 cut(s) 168, 213, 232, 252, 279, 398, 462, 890
BspCNI CTCAG 5 cut(s) 124, 1042, 1354, 1493, 2325
BspEI TCCGGA 1 cut(s) 2164
BspFNI CGCG 3 cut(s) 252, 890, 2143
BspHI TCATGA 1 cut(s) 346
BspLI GGNNCC 5 cut(s) 308, 658, 756, 1662, 1905
BspMAI CTGCAG 3 cut(s) 546, 694, 1468
BspMI ACCTGC 1 cut(s) 845
BspOI GCTAGC 1 cut(s) 1553
BspPI GGATC 2 cut(s) 36, 195
BspT107I GGYRCC 1 cut(s) 1660
BspTI CTTAAG 1 cut(s) 1244
BspTNI GGTCTC 2 cut(s) 288, 455
BsrDI GCAATG 2 cut(s) 978, 2187
BsrFI RCCGGY 1 cut(s) 74
BsrI ACTGG 7 cut(s) 156, 451, 555, 584, 745, 1306, 1533
BssAI RCCGGY 1 cut(s) 74
BssECI CCNNGG 4 cut(s) 421, 501, 2187, 2205
BssMI GATC 5 cut(s) 41, 187, 195, 316, 1861
BssNI GRCGYC 1 cut(s) 1577
BssSI CACGAG 1 cut(s) 1946
BssT1I CCWWGG 3 cut(s) 421, 2187, 2205
Bst2BI CACGAG 1 cut(s) 1946
Bst2UI CCWGG 5 cut(s) 502, 1254, 1323, 1491, 1679
Bst4CI ACNGT 9 cut(s) 371, 925, 1006, 1045, 1669, 1807, 2015, 2309, 2341
Bst6I CTCTTC 1 cut(s) 942
BstACI GRCGYC 1 cut(s) 1577
BstAFI CTTAAG 1 cut(s) 1244
BstAPI GCANNNNNTGC 1 cut(s) 857
BstC8I GCNNGC 4 cut(s) 76, 1204, 1551, 1943
BstDEI CTNAG 5 cut(s) 111, 1029, 1341, 1480, 2312
BstF5I GGATG 2 cut(s) 998, 2086
BstFNI CGCG 3 cut(s) 252, 890, 2143
BstHHI GCGC 4 cut(s) 252, 397, 2143, 2268
BstKTI GATC 5 cut(s) 44, 190, 198, 319, 1864
BstMAI GTCTC 7 cut(s) 242, 288, 455, 541, 1682, 1765, 2315
BstMBI GATC 5 cut(s) 41, 187, 195, 316, 1861
BstMCI CGRYCG 3 cut(s) 232, 256, 360
BstNI CCWGG 5 cut(s) 502, 1254, 1323, 1491, 1679
BstNSI RCATGY 3 cut(s) 485, 1945, 2094
BstSCI CCNGG 5 cut(s) 500, 1252, 1321, 1489, 1677
BstSFI CTRYAG 5 cut(s) 312, 542, 690, 1464, 2128
BstSLI GKGCMC 1 cut(s) 1665
BstUI CGCG 3 cut(s) 252, 890, 2143
BstV2I GAAGAC 2 cut(s) 345, 1181
BstX2I RGATCY 2 cut(s) 187, 1861
BstYI RGATCY 2 cut(s) 187, 1861
BstZI CGGCCG 3 cut(s) 229, 253, 357
BsuI GTATCC 1 cut(s) 1260
BtgZI GCGATG 1 cut(s) 36
BtsCI GGATG 2 cut(s) 998, 2086
BtsIMutI CAGTG 7 cut(s) 444, 1002, 1050, 1299, 1526, 1665, 1803
BveI ACCTGC 1 cut(s) 845
Cac8I GCNNGC 4 cut(s) 76, 1204, 1551, 1943
CaiI CAGNNNCTG 4 cut(s) 550, 866, 899, 1322
CciI TCATGA 1 cut(s) 346
CfoI GCGC 4 cut(s) 252, 397, 2143, 2268
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 4 cut(s) 306, 498, 755, 764
Csp6I GTAC 4 cut(s) 479, 510, 738, 1568
CviQI GTAC 4 cut(s) 479, 510, 738, 1568
DdeI CTNAG 5 cut(s) 111, 1029, 1341, 1480, 2312
DpnI GATC 5 cut(s) 43, 189, 197, 318, 1863
DpnII GATC 5 cut(s) 41, 187, 195, 316, 1861
DraI TTTAAA 1 cut(s) 1062
EaeI YGGCCR 5 cut(s) 229, 253, 357, 2184, 2383
EagI CGGCCG 3 cut(s) 229, 253, 357
Eam1104I CTCTTC 1 cut(s) 942
EarI CTCTTC 1 cut(s) 942
EciI GGCGGA 2 cut(s) 183, 228
Ecl136II GAGCTC 2 cut(s) 1600, 2123
EclXI CGGCCG 3 cut(s) 229, 253, 357
Eco130I CCWWGG 3 cut(s) 421, 2187, 2205
Eco147I AGGCCT 1 cut(s) 934
Eco24I GRGCYC 2 cut(s) 1602, 2125
Eco31I GGTCTC 2 cut(s) 288, 455
Eco47I GGWCC 1 cut(s) 755
Eco52I CGGCCG 3 cut(s) 229, 253, 357
Eco53kI GAGCTC 2 cut(s) 1600, 2123
Eco57I CTGAAG 4 cut(s) 780, 861, 1299, 1539
EcoICRI GAGCTC 2 cut(s) 1600, 2123
EcoO109I RGGNCCY 2 cut(s) 755, 764
EcoRI GAATTC 4 cut(s) 570, 1257, 1711, 2041
EcoRII CCWGG 5 cut(s) 500, 1252, 1321, 1489, 1677
EcoT14I CCWWGG 3 cut(s) 421, 2187, 2205
EcoT22I ATGCAT 2 cut(s) 618, 1943
EcoT38I GRGCYC 2 cut(s) 1602, 2125
ErhI CCWWGG 3 cut(s) 421, 2187, 2205
Esp3I CGTCTC 1 cut(s) 242
FalI AAGNNNNNCTT 2 cut(s) 1836, 1868
FaqI GGGAC 1 cut(s) 1039
FauNDI CATATG 1 cut(s) 1054
FbaI TGATCA 1 cut(s) 195
FblI GTMKAC 1 cut(s) 160
FokI GGATG 2 cut(s) 985, 2093
FriOI GRGCYC 2 cut(s) 1602, 2125
FspBI CTAG 4 cut(s) 768, 1550, 2046, 2070
GlaI GCGC 4 cut(s) 251, 396, 2142, 2267
GsaI CCCAGC 3 cut(s) 1211, 1233, 1513
GsuI CTGGAG 1 cut(s) 2015
HapII CCGG 6 cut(s) 75, 228, 360, 654, 1615, 2165
HhaI GCGC 4 cut(s) 252, 397, 2143, 2268
Hin1I GRCGYC 1 cut(s) 1577
Hin6I GCGC 4 cut(s) 250, 395, 2141, 2266
HinP1I GCGC 4 cut(s) 250, 395, 2141, 2266
HincII GTYRAC 1 cut(s) 1338
HindII GTYRAC 1 cut(s) 1338
HindIII AAGCTT 1 cut(s) 1417
HpaI GTTAAC 1 cut(s) 1338
HpaII CCGG 6 cut(s) 75, 228, 360, 654, 1615, 2165
HphI GGTGA 3 cut(s) 311, 945, 2027
Hpy166II GTNNAC 6 cut(s) 161, 623, 1152, 1338, 1675, 2135
Hpy188I TCNGA 8 cut(s) 176, 1702, 1825, 1831, 1923, 1959, 2243, 2315
Hpy8I GTNNAC 6 cut(s) 161, 623, 1152, 1338, 1675, 2135
Hpy99I CGWCG 4 cut(s) 56, 68, 2121, 2372
HpyAV CCTTC 3 cut(s) 440, 755, 1894
HpyCH4III ACNGT 9 cut(s) 371, 925, 1006, 1045, 1669, 1807, 2015, 2309, 2341
HpyCH4IV ACGT 4 cut(s) 54, 1577, 1734, 1777
HpyF3I CTNAG 5 cut(s) 111, 1029, 1341, 1480, 2312
HpySE526I ACGT 4 cut(s) 54, 1577, 1734, 1777
Hsp92I GRCGYC 1 cut(s) 1577
HspAI GCGC 4 cut(s) 250, 395, 2141, 2266
Kpn2I TCCGGA 1 cut(s) 2164
KroI GCCGGC 1 cut(s) 74
KroNI GCCGGC 1 cut(s) 76
Ksp22I TGATCA 1 cut(s) 195
KspAI GTTAAC 1 cut(s) 1338
Kzo9I GATC 5 cut(s) 41, 187, 195, 316, 1861
LmnI GCTCC 4 cut(s) 1226, 1324, 1629, 2191
LweI GCATC 4 cut(s) 352, 574, 603, 2071
MaeI CTAG 4 cut(s) 768, 1550, 2046, 2070
MaeII ACGT 4 cut(s) 54, 1577, 1734, 1777
MaeIII GTNAC 3 cut(s) 55, 586, 727
MalI GATC 5 cut(s) 43, 189, 197, 318, 1863
MboI GATC 5 cut(s) 41, 187, 195, 316, 1861
MflI RGATCY 2 cut(s) 187, 1861
MhlI GDGCHC 3 cut(s) 1602, 1665, 2125
MlsI TGGCCA 2 cut(s) 2186, 2385
MluNI TGGCCA 2 cut(s) 2186, 2385
MlyI GAGTC 7 cut(s) 180, 359, 1021, 1126, 1820, 1912, 1960
MmeI TCCRAC 5 cut(s) 72, 154, 1513, 1624, 1748
Mox20I TGGCCA 2 cut(s) 2186, 2385
Mph1103I ATGCAT 2 cut(s) 618, 1943
MreI CGCCGGCG 1 cut(s) 74
MroI TCCGGA 1 cut(s) 2164
MroNI GCCGGC 1 cut(s) 74
MroXI GAANNNNTTC 4 cut(s) 874, 1819, 1851, 1989
MscI TGGCCA 2 cut(s) 2186, 2385
Msp20I TGGCCA 2 cut(s) 2186, 2385
MspA1I CMGCKG 4 cut(s) 725, 866, 1463, 1742
MspCI CTTAAG 1 cut(s) 1244
MspI CCGG 6 cut(s) 75, 228, 360, 654, 1615, 2165
MspR9I CCNGG 5 cut(s) 502, 1254, 1323, 1491, 1679
Mva1269I GAATGC 1 cut(s) 1107
MvaI CCWGG 5 cut(s) 502, 1254, 1323, 1491, 1679
MvnI CGCG 3 cut(s) 252, 890, 2143
NaeI GCCGGC 1 cut(s) 76
NdeI CATATG 1 cut(s) 1054
NdeII GATC 5 cut(s) 41, 187, 195, 316, 1861
NgoMIV GCCGGC 1 cut(s) 74
NheI GCTAGC 1 cut(s) 1549
NlaIV GGNNCC 5 cut(s) 308, 658, 756, 1662, 1905
NmeAIII GCCGAG 2 cut(s) 281, 414
NmuCI GTSAC 2 cut(s) 586, 727
NsiI ATGCAT 2 cut(s) 618, 1943
NspI RCATGY 3 cut(s) 485, 1945, 2094
PaeI GCATGC 1 cut(s) 1945
PagI TCATGA 1 cut(s) 346
PaqCI CACCTGC 1 cut(s) 845
PceI AGGCCT 1 cut(s) 934
PciI ACATGT 2 cut(s) 481, 2090
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PctI GAATGC 1 cut(s) 1107
PdiI GCCGGC 1 cut(s) 76
PdmI GAANNNNTTC 4 cut(s) 874, 1819, 1851, 1989
PfeI GAWTC 6 cut(s) 28, 801, 1326, 2271, 2294, 2362
PflFI GACNNNGTC 1 cut(s) 1030
PflMI CCANNNNNTGG 2 cut(s) 275, 521
PfoI TCCNGGA 3 cut(s) 1252, 1321, 1489
PleI GAGTC 7 cut(s) 179, 358, 1021, 1126, 1820, 1912, 1959
PpsI GAGTC 7 cut(s) 179, 358, 1021, 1126, 1820, 1912, 1959
PpuMI RGGWCCY 1 cut(s) 755
PscI ACATGT 2 cut(s) 481, 2090
PshBI ATTAAT 2 cut(s) 122, 809
Psp124BI GAGCTC 2 cut(s) 1602, 2125
Psp5II RGGWCCY 1 cut(s) 755
Psp6I CCWGG 5 cut(s) 500, 1252, 1321, 1489, 1677
PspFI CCCAGC 3 cut(s) 1207, 1229, 1509
PspGI CCWGG 5 cut(s) 500, 1252, 1321, 1489, 1677
PspN4I GGNNCC 5 cut(s) 308, 658, 756, 1662, 1905
PspPI GGNCC 4 cut(s) 306, 498, 755, 764
PspPPI RGGWCCY 1 cut(s) 755
PsrI GAACNNNNNNTAC 2 cut(s) 730, 762
PstI CTGCAG 3 cut(s) 546, 694, 1468
PstNI CAGNNNCTG 4 cut(s) 550, 866, 899, 1322
PsuI RGATCY 2 cut(s) 187, 1861
PsyI GACNNNGTC 1 cut(s) 1030
PvuII CAGCTG 4 cut(s) 725, 866, 1463, 1742
RsaI GTAC 4 cut(s) 480, 511, 739, 1569
RsaNI GTAC 4 cut(s) 479, 510, 738, 1568
SacI GAGCTC 2 cut(s) 1602, 2125
Sau3AI GATC 5 cut(s) 41, 187, 195, 316, 1861
Sau96I GGNCC 4 cut(s) 306, 498, 755, 764
SchI GAGTC 7 cut(s) 180, 359, 1021, 1126, 1820, 1912, 1960
ScrFI CCNGG 5 cut(s) 502, 1254, 1323, 1491, 1679
SduI GDGCHC 3 cut(s) 1602, 1665, 2125
SfaNI GCATC 4 cut(s) 352, 574, 603, 2071
SfcI CTRYAG 5 cut(s) 312, 542, 690, 1464, 2128
SgrAI CRCCGGYG 1 cut(s) 74
SinI GGWCC 1 cut(s) 755
SmlI CTYRAG 2 cut(s) 1244, 1875
SmoI CTYRAG 2 cut(s) 1244, 1875
SphI GCATGC 1 cut(s) 1945
SseBI AGGCCT 1 cut(s) 934
SsiI CCGC 8 cut(s) 168, 213, 232, 252, 279, 398, 462, 890
SspI AATATT 1 cut(s) 633
SspMI CTAG 4 cut(s) 768, 1550, 2046, 2070
SstI GAGCTC 2 cut(s) 1602, 2125
StuI AGGCCT 1 cut(s) 934
StyD4I CCNGG 5 cut(s) 500, 1252, 1321, 1489, 1677
StyI CCWWGG 3 cut(s) 421, 2187, 2205
TaaI ACNGT 9 cut(s) 371, 925, 1006, 1045, 1669, 1807, 2015, 2309, 2341
TaiI ACGT 4 cut(s) 57, 1580, 1737, 1780
TaqI TCGA 7 cut(s) 568, 627, 940, 1135, 2036, 2116, 2367
TauI GCSGC 5 cut(s) 234, 255, 400, 464, 893
TfiI GAWTC 6 cut(s) 28, 801, 1326, 2271, 2294, 2362
TscAI CASTG 7 cut(s) 451, 1009, 1050, 1306, 1533, 1672, 1810
TseFI GTSAC 2 cut(s) 586, 727
Tsp45I GTSAC 2 cut(s) 586, 727
TspDTI ATGAA 6 cut(s) 507, 1761, 1998, 2091, 2276, 2333
TspRI CASTG 7 cut(s) 451, 1009, 1050, 1306, 1533, 1672, 1810
Tth111I GACNNNGTC 1 cut(s) 1030
Van91I CCANNNNNTGG 2 cut(s) 275, 521
Vha464I CTTAAG 1 cut(s) 1244
VpaK11BI GGWCC 1 cut(s) 755
VspI ATTAAT 2 cut(s) 122, 809
XapI RAATTY 6 cut(s) 570, 870, 1257, 1290, 1711, 2041
XceI RCATGY 3 cut(s) 485, 1945, 2094
XcmI CCANNNNNNNNNTGG 1 cut(s) 2194
XmiI GTMKAC 1 cut(s) 160
XmnI GAANNNNTTC 4 cut(s) 874, 1819, 1851, 1989
XspI CTAG 4 cut(s) 768, 1550, 2046, 2070
ZraI GACGTC 1 cut(s) 1578
Zsp2I ATGCAT 2 cut(s) 618, 1943
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.