RLG00000036476

Chloride channel protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0008013
Physical Location & Seq
Reverse (-)
81083783 .. 81088907
5125 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036476

Sequence Viewer

Length: 2391 bp
ATGTCAGGAGAGGAGTACAGCGATGAGACTCATCTGCTGAGATCCTCGAACGACATTAATAAAGACGAGAACGGCGCCGTTTCGCCGGAGGACGACAACGACGGCGATGGCGACGGCGACTTGGAAGCGCAGAGCCGGAAAAGTAGTATCAGTCCGAGCAGTAGAGTCGGTGCTTTAAAGGATCTGCTGCTGAAGCATTTGGACGGCGTCGGACGGCTTTCCGGTCGCCGGAACAGTTTCAAGAGGCTCGATGGGAATTGGGATAGGGATAGGGGTAGGGAGAGTAATCGGAGAGAAAGTAGAGAGCGGCGATCGCCTGTAGATCAGCTGCGTCATAGTAGTAATCATGTCGCGCCGGCCGGAATCGATGGGAACGATGATGAACTGGCCGATAGTGCTCCGCCGGAGTGGGCTATGCTTCTCCTCGGTTGCATTCTCGGCCTCGCCACCGGTCTCTTTGTTGCCGCGTTTAACAAAGGAGTACATGTTATACACGAATGGGCCTGGGCTGGTACTCCAACTGAAGGTGCTGCATGGCTCCGTCTGCAGAGACTGGGTGATACTTGGCATCGAATACTTTTAGTACCCGTCACAGGTGGGGTTATTGTTGGCATGATGCATGGTTTACTTGAAATATTGGATCAAATAAGACAGTTAAGTTCCTCCCAAGGGAAAGGATTTGATTTACTAGCTGGAGTCTTTCCCACAATAAAGGCCGTCCAGGCAGCTATCACACTAGGTACTGGTTGTTCTTTAGGTCCTGAAGGCCCTAGTGTAGATATTGGAAAATCATGTGCCAACGGGTTCTCATTAATGATGGAAAACAACAGAGAAAGGAAGATTGCCCTCATTGCAGCAGGTGCTGCTGCTGGAATTTCTTCAGGATTTAATGCGGCAGTTGCTGGTTGTTTCTTTGCTATTGAAACGGTGCTAAGGCCACTTCGAGCAGAAAACTCTCCTCCTTTTACTACCGCAATGATTATATTGGCTTCTGTTATATCGTCCACAGTATCTAATGTTTTACTTGGGACGCAATCAGCCTTTACAGTGCCTGTTTACGATTTGAAATCAGCTGCTGAATTGCCCCTCTACCTTATATTGGGCATGCTATGTGGTGCTGTGAGTGTAGTCTTCAATCGCTTGGTTGCTTGGTTCACAAAGTTTTTTGATTATATCAAGGAAAGATTTGGCCTTCCTGCGGTTGCATGCCCTGCTTTAGGTGGTTTAGGAGCTGGGTTGATTGCTCTCAAGTATCCGGGAATTCTGTACTGGGGTTTCACAAATGTTGAAGAAATCCTGCATACTGGAAAGACTGCTTCCGCCCCCGGAATCTGGCTGTTAACTCAATTATCAGCAGCCAAAGTTGTAGCAACAGCTTTATGCAAGGGTTCTGGGCTTGTAGGTGGACTTTATGCACCAAGCTTGATGATTGGGGCTGCTGTTGGCGCTGTATTTGGGGGTTCAGCTGCAGAATTGATTAATTCAGCAATTCCAGGAAATGCTGCCGTTGCTCAGCCACAGGCATATGCACTGGTGGGAATGGCTGCTATGCTGGCTTCAGTTTGTTCGGTGCCATTGACGTCAGTTCTCCTTCTGTTTGAGCTGACAAAAGATTATCGAATATTGCTTCCTCTCATGGGGGCTGTTGGATTGGCAATTTGGGTTCCTTCTGTCGTAAACCAGGGAAAGGAGACTGAAGCATCTGATACACGGAATTCTGCGAGAGGTTATTCTTCTGTTTCAGCTGCTGAAGAGAAAGATGAAGTTATTTGGAGGCAACATGGTAGTGGAGATGATATGGAGCTGTCTACTATGGGGAATACTTCTGATTCTGAATTAACTGAAGAAATGCTTCTGGAGAATCTAAAGGTTTCGCGGGCCATGTCAAAGAATTATGTGAAGGTTTCCCTGACCGTAACTATTGAAGAGGCAATAAAATCCATGCATGACAATCATCAGAATTGTGTGTTAATTGTTGATGATGAAGATTTTCTGGATGGAGTATTGACATATGGTGATGTTAGACGGTATCAATCAAGGAAGTCTTCCGATACGTTGAAGAGCGATTCTAGTTTTCTGGATGAAAATACCTGTCTTGTTTCCTCTGTATGTACTCGAGAAATCAGCTTTCATGGGCGAACCCGAGGTCTATTAACCTGTTATCCGGACACGGGTTTATTAATGGCCAAGGAGCTAATGGAGGCAAAAGATATCAAGCAGTTGCCTGTGGTTAAGCGTGGCAGACAACCACCAAAAGAAACGAGGCGAAGACTTGTTGCTATTCTTCATTACGATTCAATCTTGATGTGTCTCAGGGAGGAGATAAATCACAGGAAATCAATTCATCAACATAGAAAAGAGAATCTTGACGAAATAACAAATGGCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0009507 GO:0009536 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBS Voltage_CLC

Protein Analysis

797

Amino Acids

85.79

Weight (kDa)

5.83

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Voltage_CLC PF00654 225 - 555 1.1e-70 Voltage gated chloride channel
CBS PF00571 624 - 678 5.5e-08 CBS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 848
AatII GACGTC 1 cut(s) 1583
Acc36I ACCTGC 1 cut(s) 848
AccB1I GGYRCC 2 cut(s) 74, 1570
AccBSI CCGCTC 1 cut(s) 307
AccI GTMKAC 1 cut(s) 1808
AccII CGCG 3 cut(s) 353, 467, 1876
AccIII TCCGGA 1 cut(s) 2164
AciI CCGC 8 cut(s) 307, 401, 465, 893, 972, 1199, 1320, 1876
AclWI GGATC 3 cut(s) 36, 189, 648
AcoI YGGCCR 4 cut(s) 357, 387, 2184, 2383
AcsI RAATTY 3 cut(s) 873, 1260, 1714
AcuI CTGAAG 8 cut(s) 212, 543, 783, 864, 1542, 1716, 1770, 1863
AcyI GRCGYC 3 cut(s) 75, 207, 1580
AfaI GTAC 7 cut(s) 17, 483, 514, 585, 742, 1268, 2113
AflIII ACRYGT 1 cut(s) 484
AgeI ACCGGT 1 cut(s) 449
AgsI TTSAA 9 cut(s) 241, 632, 923, 1066, 1135, 1289, 1925, 2059, 2298
AjnI CCWGG 4 cut(s) 503, 720, 1492, 1680
Alw21I GWGCWC 1 cut(s) 400
Alw26I GTCTC 5 cut(s) 20, 458, 544, 1685, 2315
AlwI GGATC 3 cut(s) 36, 189, 648
AlwNI CAGNNNCTG 6 cut(s) 553, 863, 902, 1052, 1076, 1748
Ama87I CYCGRG 2 cut(s) 2115, 2142
Aor13HI TCCGGA 1 cut(s) 2164
ApoI RAATTY 3 cut(s) 873, 1260, 1714
ArsI GACNNNNNNTTYG 2 cut(s) 2131, 2163
AseI ATTAAT 4 cut(s) 57, 812, 1479, 2180
AsiGI ACCGGT 1 cut(s) 449
AsiSI GCGATCGC 1 cut(s) 314
Asp700I GAANNNNTTC 4 cut(s) 236, 877, 1851, 1989
AspLEI GCGC 4 cut(s) 77, 130, 355, 1448
AspS9I GGNCC 4 cut(s) 501, 758, 767, 1878
AsuC2I CCSGG 2 cut(s) 1257, 1326
AsuHPI GGTGA 2 cut(s) 569, 2027
AvaI CYCGRG 2 cut(s) 2115, 2142
AvaII GGWCC 1 cut(s) 758
BalI TGGCCA 2 cut(s) 2186, 2385
BanI GGYRCC 2 cut(s) 74, 1570
BbsI GAAGAC 3 cut(s) 1123, 2037, 2275
Bbv12I GWGCWC 1 cut(s) 400
BccI CCATC 5 cut(s) 101, 245, 362, 811, 1991
BceAI ACGGC 8 cut(s) 62, 88, 118, 130, 220, 230, 701, 1490
BcgI CGANNNNNNTGC 2 cut(s) 148, 182
BciT130I CCWGG 4 cut(s) 505, 722, 1494, 1682
BciVI GTATCC 1 cut(s) 1263
BcnI CCSGG 2 cut(s) 1257, 1326
BcoDI GTCTC 5 cut(s) 20, 458, 544, 1685, 2315
BfaI CTAG 4 cut(s) 689, 737, 771, 2070
BfmI CTRYAG 3 cut(s) 318, 545, 1467
BfoI RGCGCY 2 cut(s) 78, 1449
BfuAI ACCTGC 1 cut(s) 848
BfuI GTATCC 1 cut(s) 1263
BglI GCCNNNNNGGC 1 cut(s) 722
BlpI GCTNAGC 1 cut(s) 1512
Bme1390I CCNGG 6 cut(s) 505, 722, 1257, 1326, 1494, 1682
Bme18I GGWCC 1 cut(s) 758
BmeT110I CYCGRG 2 cut(s) 2115, 2142
BmgT120I GGNCC 4 cut(s) 501, 758, 767, 1878
BmiI GGNNCC 4 cut(s) 76, 539, 1572, 1665
BmrFI CCNGG 6 cut(s) 505, 722, 1257, 1326, 1494, 1682
BmrI ACTGGG 2 cut(s) 563, 1279
BmsI GCATC 3 cut(s) 577, 606, 1709
BmuI ACTGGG 2 cut(s) 563, 1279
BpiI GAAGAC 3 cut(s) 1123, 2037, 2275
BpmI CTGGAG 2 cut(s) 714, 1877
Bpu10I CCTNAGC 1 cut(s) 932
Bpu1102I GCTNAGC 1 cut(s) 1512
BpuEI CTTGAG 1 cut(s) 1232
BpuMI CCSGG 2 cut(s) 1257, 1326
Bsa29I ATCGAT 1 cut(s) 366
BsaHI GRCGYC 3 cut(s) 75, 207, 1580
BsaI GGTCTC 1 cut(s) 458
BsaJI CCNNGG 7 cut(s) 424, 504, 667, 1324, 1681, 2143, 2187
BsaWI WCCGGW 3 cut(s) 221, 449, 2164
BsaXI ACNNNNNCTCC 4 cut(s) 283, 313, 471, 501
Bse118I RCCGGY 2 cut(s) 355, 449
Bse1I ACTGG 6 cut(s) 390, 558, 748, 1274, 1309, 1536
Bse3DI GCAATG 2 cut(s) 849, 981
BseAI TCCGGA 1 cut(s) 2164
BseBI CCWGG 4 cut(s) 505, 722, 1494, 1682
BseCI ATCGAT 1 cut(s) 366
BseDI CCNNGG 7 cut(s) 424, 504, 667, 1324, 1681, 2143, 2187
BseGI GGATG 2 cut(s) 2002, 2086
BseMI GCAATG 2 cut(s) 849, 981
BseMII CTCAG 3 cut(s) 29, 1526, 2326
BseNI ACTGG 6 cut(s) 390, 558, 748, 1274, 1309, 1536
BseRI GAGGAG 4 cut(s) 26, 413, 948, 2333
BseX3I CGGCCG 1 cut(s) 357
BseYI CCCAGC 1 cut(s) 1232
Bsh1236I CGCG 3 cut(s) 353, 467, 1876
Bsh1285I CGRYCG 3 cut(s) 226, 314, 360
BshNI GGYRCC 2 cut(s) 74, 1570
BshTI ACCGGT 1 cut(s) 449
BshVI ATCGAT 1 cut(s) 366
BsiEI CGRYCG 3 cut(s) 226, 314, 360
BsiHKAI GWGCWC 1 cut(s) 400
BsiHKCI CYCGRG 2 cut(s) 2115, 2142
BslFI GGGAC 1 cut(s) 1042
BsmAI GTCTC 5 cut(s) 20, 458, 544, 1685, 2315
BsmFI GGGAC 1 cut(s) 1042
BsmI GAATGC 1 cut(s) 432
Bso31I GGTCTC 1 cut(s) 458
BsoBI CYCGRG 2 cut(s) 2115, 2142
Bsp1286I GDGCHC 1 cut(s) 400
Bsp13I TCCGGA 1 cut(s) 2164
Bsp143I GATC 5 cut(s) 41, 181, 311, 322, 640
Bsp1720I GCTNAGC 1 cut(s) 1512
BspACI CCGC 8 cut(s) 307, 401, 465, 893, 972, 1199, 1320, 1876
BspCNI CTCAG 3 cut(s) 30, 1525, 2325
BspDI ATCGAT 1 cut(s) 366
BspEI TCCGGA 1 cut(s) 2164
BspFNI CGCG 3 cut(s) 353, 467, 1876
BspLI GGNNCC 4 cut(s) 76, 539, 1572, 1665
BspMAI CTGCAG 2 cut(s) 549, 1471
BspMI ACCTGC 1 cut(s) 848
BspPI GGATC 3 cut(s) 36, 189, 648
BspQI GCTCTTC 1 cut(s) 2054
BspT107I GGYRCC 2 cut(s) 74, 1570
BspTNI GGTCTC 1 cut(s) 458
BsrBI CCGCTC 1 cut(s) 307
BsrDI GCAATG 2 cut(s) 849, 981
BsrFI RCCGGY 2 cut(s) 355, 449
BsrI ACTGG 6 cut(s) 390, 558, 748, 1274, 1309, 1536
BssAI RCCGGY 2 cut(s) 355, 449
BssECI CCNNGG 7 cut(s) 424, 504, 667, 1324, 1681, 2143, 2187
BssMI GATC 5 cut(s) 41, 181, 311, 322, 640
BssNI GRCGYC 3 cut(s) 75, 207, 1580
BssT1I CCWWGG 2 cut(s) 667, 2187
Bst2UI CCWGG 4 cut(s) 505, 722, 1494, 1682
Bst4CI ACNGT 7 cut(s) 236, 654, 928, 1009, 1048, 1915, 2028
Bst6I CTCTTC 3 cut(s) 1746, 1920, 2054
BstACI GRCGYC 3 cut(s) 75, 207, 1580
BstAPI GCANNNNNTGC 3 cut(s) 860, 863, 1211
BstC8I GCNNGC 5 cut(s) 357, 1106, 1207, 1554, 1878
BstDEI CTNAG 4 cut(s) 38, 932, 1512, 2312
BstENI CCTNNNNNAGG 1 cut(s) 1215
BstF5I GGATG 2 cut(s) 2002, 2086
BstFNI CGCG 3 cut(s) 353, 467, 1876
BstH2I RGCGCY 2 cut(s) 78, 1449
BstHHI GCGC 4 cut(s) 77, 130, 355, 1448
BstKTI GATC 5 cut(s) 44, 184, 314, 325, 643
BstMAI GTCTC 5 cut(s) 20, 458, 544, 1685, 2315
BstMBI GATC 5 cut(s) 41, 181, 311, 322, 640
BstMCI CGRYCG 3 cut(s) 226, 314, 360
BstNI CCWGG 4 cut(s) 505, 722, 1494, 1682
BstNSI RCATGY 3 cut(s) 488, 1108, 1209
BstSCI CCNGG 6 cut(s) 503, 720, 1255, 1324, 1492, 1680
BstSFI CTRYAG 3 cut(s) 318, 545, 1467
BstUI CGCG 3 cut(s) 353, 467, 1876
BstV2I GAAGAC 3 cut(s) 1123, 2037, 2275
BstX2I RGATCY 2 cut(s) 41, 181
BstYI RGATCY 2 cut(s) 41, 181
BstZI CGGCCG 1 cut(s) 357
Bsu15I ATCGAT 1 cut(s) 366
BsuI GTATCC 1 cut(s) 1263
BsuTUI ATCGAT 1 cut(s) 366
BtgZI GCGATG 2 cut(s) 36, 120
BtsCI GGATG 2 cut(s) 2002, 2086
BtsIMutI CAGTG 2 cut(s) 1053, 1529
BveI ACCTGC 1 cut(s) 848
Cac8I GCNNGC 5 cut(s) 357, 1106, 1207, 1554, 1878
CaiI CAGNNNCTG 6 cut(s) 553, 863, 902, 1052, 1076, 1748
CfoI GCGC 4 cut(s) 77, 130, 355, 1448
Cfr10I RCCGGY 2 cut(s) 355, 449
Cfr13I GGNCC 4 cut(s) 501, 758, 767, 1878
ClaI ATCGAT 1 cut(s) 366
CseI GACGC 3 cut(s) 196, 320, 1039
Csp6I GTAC 7 cut(s) 16, 482, 513, 584, 741, 1267, 2112
CspAI ACCGGT 1 cut(s) 449
CviQI GTAC 7 cut(s) 16, 482, 513, 584, 741, 1267, 2112
DdeI CTNAG 4 cut(s) 38, 932, 1512, 2312
DinI GGCGCC 1 cut(s) 76
DpnI GATC 5 cut(s) 43, 183, 313, 324, 642
DpnII GATC 5 cut(s) 41, 181, 311, 322, 640
DraI TTTAAA 1 cut(s) 177
EaeI YGGCCR 4 cut(s) 357, 387, 2184, 2383
EagI CGGCCG 1 cut(s) 357
Eam1104I CTCTTC 3 cut(s) 1746, 1920, 2054
EarI CTCTTC 3 cut(s) 1746, 1920, 2054
EciI GGCGGA 2 cut(s) 390, 1309
EclXI CGGCCG 1 cut(s) 357
Eco130I CCWWGG 2 cut(s) 667, 2187
Eco31I GGTCTC 1 cut(s) 458
Eco32I GATATC 1 cut(s) 2212
Eco47I GGWCC 1 cut(s) 758
Eco52I CGGCCG 1 cut(s) 357
Eco57I CTGAAG 8 cut(s) 212, 543, 783, 864, 1542, 1716, 1770, 1863
Eco88I CYCGRG 2 cut(s) 2115, 2142
EcoNI CCTNNNNNAGG 1 cut(s) 1215
EcoO109I RGGNCCY 2 cut(s) 758, 767
EcoRI GAATTC 2 cut(s) 1260, 1714
EcoRII CCWGG 4 cut(s) 503, 720, 1492, 1680
EcoRV GATATC 1 cut(s) 2212
EcoT14I CCWWGG 2 cut(s) 667, 2187
EcoT22I ATGCAT 2 cut(s) 621, 1947
EgeI GGCGCC 1 cut(s) 76
EheI GGCGCC 1 cut(s) 76
ErhI CCWWGG 2 cut(s) 667, 2187
FalI AAGNNNNNCTT 6 cut(s) 1836, 1868, 2029, 2061, 2349, 2381
FaqI GGGAC 1 cut(s) 1042
FauI CCCGC 1 cut(s) 1869
FauNDI CATATG 2 cut(s) 1525, 2011
FblI GTMKAC 1 cut(s) 1808
FokI GGATG 2 cut(s) 2009, 2093
FspBI CTAG 4 cut(s) 689, 737, 771, 2070
GlaI GCGC 4 cut(s) 76, 129, 354, 1447
GsaI CCCAGC 1 cut(s) 1236
GsuI CTGGAG 2 cut(s) 714, 1877
HaeII RGCGCY 2 cut(s) 78, 1449
HgaI GACGC 3 cut(s) 196, 320, 1039
HhaI GCGC 4 cut(s) 77, 130, 355, 1448
Hin1I GRCGYC 3 cut(s) 75, 207, 1580
Hin6I GCGC 4 cut(s) 75, 128, 353, 1446
HinP1I GCGC 4 cut(s) 75, 128, 353, 1446
HincII GTYRAC 1 cut(s) 1341
HindII GTYRAC 1 cut(s) 1341
HindIII AAGCTT 1 cut(s) 1420
HpaI GTTAAC 1 cut(s) 1341
HphI GGTGA 2 cut(s) 569, 2027
Hpy166II GTNNAC 8 cut(s) 626, 1005, 1057, 1155, 1341, 1406, 1678, 1809
Hpy188I TCNGA 8 cut(s) 156, 212, 291, 1705, 1828, 1834, 1959, 2050
Hpy8I GTNNAC 8 cut(s) 626, 1005, 1057, 1155, 1341, 1406, 1678, 1809
Hpy99I CGWCG 3 cut(s) 104, 116, 212
HpyAV CCTTC 6 cut(s) 518, 758, 1202, 1601, 1677, 1894
HpyCH4III ACNGT 7 cut(s) 236, 654, 928, 1009, 1048, 1915, 2028
HpyCH4IV ACGT 2 cut(s) 1580, 2054
HpyF3I CTNAG 4 cut(s) 38, 932, 1512, 2312
HpySE526I ACGT 2 cut(s) 1580, 2054
Hsp92I GRCGYC 3 cut(s) 75, 207, 1580
HspAI GCGC 4 cut(s) 75, 128, 353, 1446
KasI GGCGCC 1 cut(s) 74
Kpn2I TCCGGA 1 cut(s) 2164
KroI GCCGGC 1 cut(s) 355
KroNI GCCGGC 1 cut(s) 357
KspAI GTTAAC 1 cut(s) 1341
Kzo9I GATC 5 cut(s) 41, 181, 311, 322, 640
LguI GCTCTTC 1 cut(s) 2054
LmnI GCTCC 5 cut(s) 403, 543, 1229, 1801, 2191
LweI GCATC 3 cut(s) 577, 606, 1709
MaeI CTAG 4 cut(s) 689, 737, 771, 2070
MaeII ACGT 2 cut(s) 1580, 2054
MaeIII GTNAC 2 cut(s) 589, 1915
MalI GATC 5 cut(s) 43, 183, 313, 324, 642
MbiI CCGCTC 1 cut(s) 307
MboI GATC 5 cut(s) 41, 181, 311, 322, 640
MflI RGATCY 2 cut(s) 41, 181
MhlI GDGCHC 1 cut(s) 400
MlsI TGGCCA 2 cut(s) 2186, 2385
MluNI TGGCCA 2 cut(s) 2186, 2385
Mly113I GGCGCC 1 cut(s) 75
MlyI GAGTC 3 cut(s) 22, 174, 705
MmeI TCCRAC 3 cut(s) 190, 542, 1627
Mox20I TGGCCA 2 cut(s) 2186, 2385
Mph1103I ATGCAT 2 cut(s) 621, 1947
MroI TCCGGA 1 cut(s) 2164
MroNI GCCGGC 1 cut(s) 355
MroXI GAANNNNTTC 4 cut(s) 236, 877, 1851, 1989
MscI TGGCCA 2 cut(s) 2186, 2385
MslI CAYNNNNRTG 1 cut(s) 1785
Msp20I TGGCCA 2 cut(s) 2186, 2385
MspA1I CMGCKG 4 cut(s) 328, 1073, 1466, 1745
MspR9I CCNGG 6 cut(s) 505, 722, 1257, 1326, 1494, 1682
Mva1269I GAATGC 1 cut(s) 432
MvaI CCWGG 4 cut(s) 505, 722, 1494, 1682
MvnI CGCG 3 cut(s) 353, 467, 1876
NaeI GCCGGC 1 cut(s) 357
NarI GGCGCC 1 cut(s) 75
NciI CCSGG 2 cut(s) 1257, 1326
NdeI CATATG 2 cut(s) 1525, 2011
NdeII GATC 5 cut(s) 41, 181, 311, 322, 640
NgoMIV GCCGGC 1 cut(s) 355
NlaIV GGNNCC 4 cut(s) 76, 539, 1572, 1665
NmeAIII GCCGAG 1 cut(s) 417
NmuCI GTSAC 1 cut(s) 589
NsiI ATGCAT 2 cut(s) 621, 1947
NspI RCATGY 3 cut(s) 488, 1108, 1209
PaeI GCATGC 2 cut(s) 1108, 1209
PaeR7I CTCGAG 1 cut(s) 2115
PaqCI CACCTGC 1 cut(s) 848
PciI ACATGT 1 cut(s) 484
PciSI GCTCTTC 1 cut(s) 2054
PcsI WCGNNNNNNNCGW 2 cut(s) 108, 372
PctI GAATGC 1 cut(s) 432
PdiI GCCGGC 1 cut(s) 357
PdmI GAANNNNTTC 4 cut(s) 236, 877, 1851, 1989
PfeI GAWTC 7 cut(s) 363, 1329, 1829, 1861, 2066, 2294, 2362
PflFI GACNNNGTC 1 cut(s) 206
PfoI TCCNGGA 2 cut(s) 1255, 1492
PinAI ACCGGT 1 cut(s) 449
Ple19I CGATCG 1 cut(s) 314
PleI GAGTC 3 cut(s) 22, 173, 704
PluTI GGCGCC 1 cut(s) 78
PpsI GAGTC 3 cut(s) 22, 173, 704
PpuMI RGGWCCY 1 cut(s) 758
PscI ACATGT 1 cut(s) 484
PshBI ATTAAT 4 cut(s) 57, 812, 1479, 2180
Psp5II RGGWCCY 1 cut(s) 758
Psp6I CCWGG 4 cut(s) 503, 720, 1492, 1680
PspFI CCCAGC 1 cut(s) 1232
PspGI CCWGG 4 cut(s) 503, 720, 1492, 1680
PspN4I GGNNCC 4 cut(s) 76, 539, 1572, 1665
PspPI GGNCC 4 cut(s) 501, 758, 767, 1878
PspPPI RGGWCCY 1 cut(s) 758
PsrI GAACNNNNNNTAC 2 cut(s) 733, 765
PstI CTGCAG 2 cut(s) 549, 1471
PstNI CAGNNNCTG 6 cut(s) 553, 863, 902, 1052, 1076, 1748
PsuI RGATCY 2 cut(s) 41, 181
PsyI GACNNNGTC 1 cut(s) 206
PvuI CGATCG 1 cut(s) 314
PvuII CAGCTG 4 cut(s) 328, 1073, 1466, 1745
RgaI GCGATCGC 1 cut(s) 314
RsaI GTAC 7 cut(s) 17, 483, 514, 585, 742, 1268, 2113
RsaNI GTAC 7 cut(s) 16, 482, 513, 584, 741, 1267, 2112
RseI CAYNNNNRTG 1 cut(s) 1785
SapI GCTCTTC 1 cut(s) 2054
Sau3AI GATC 5 cut(s) 41, 181, 311, 322, 640
Sau96I GGNCC 4 cut(s) 501, 758, 767, 1878
SchI GAGTC 3 cut(s) 22, 174, 705
ScrFI CCNGG 6 cut(s) 505, 722, 1257, 1326, 1494, 1682
SduI GDGCHC 1 cut(s) 400
SfaAI GCGATCGC 1 cut(s) 314
SfaNI GCATC 3 cut(s) 577, 606, 1709
SfcI CTRYAG 3 cut(s) 318, 545, 1467
SfoI GGCGCC 1 cut(s) 76
Sfr274I CTCGAG 1 cut(s) 2115
SgfI GCGATCGC 1 cut(s) 314
SinI GGWCC 1 cut(s) 758
SlaI CTCGAG 1 cut(s) 2115
SmiMI CAYNNNNRTG 1 cut(s) 1785
SmlI CTYRAG 2 cut(s) 1247, 2115
SmoI CTYRAG 2 cut(s) 1247, 2115
SphI GCATGC 2 cut(s) 1108, 1209
SsiI CCGC 8 cut(s) 307, 401, 465, 893, 972, 1199, 1320, 1876
SspDI GGCGCC 1 cut(s) 74
SspI AATATT 2 cut(s) 636, 1623
SspMI CTAG 4 cut(s) 689, 737, 771, 2070
StyD4I CCNGG 6 cut(s) 503, 720, 1255, 1324, 1492, 1680
StyI CCWWGG 2 cut(s) 667, 2187
TaaI ACNGT 7 cut(s) 236, 654, 928, 1009, 1048, 1915, 2028
TaiI ACGT 2 cut(s) 1583, 2057
TaqI TCGA 7 cut(s) 47, 249, 366, 571, 943, 1618, 2116
TatI WGTACW 4 cut(s) 15, 481, 1266, 2111
TauI GCSGC 3 cut(s) 310, 467, 896
TfiI GAWTC 7 cut(s) 363, 1329, 1829, 1861, 2066, 2294, 2362
TscAI CASTG 2 cut(s) 1053, 1536
TseFI GTSAC 1 cut(s) 589
Tsp45I GTSAC 1 cut(s) 589
TspDTI ATGAA 7 cut(s) 396, 1776, 1998, 2097, 2120, 2276, 2333
TspGWI ACGGA 2 cut(s) 530, 1726
TspRI CASTG 2 cut(s) 1053, 1536
Tth111I GACNNNGTC 1 cut(s) 206
VpaK11BI GGWCC 1 cut(s) 758
VspI ATTAAT 4 cut(s) 57, 812, 1479, 2180
XagI CCTNNNNNAGG 1 cut(s) 1215
XapI RAATTY 3 cut(s) 873, 1260, 1714
XceI RCATGY 3 cut(s) 488, 1108, 1209
XcmI CCANNNNNNNNNTGG 1 cut(s) 2194
XhoI CTCGAG 1 cut(s) 2115
XmiI GTMKAC 1 cut(s) 1808
XmnI GAANNNNTTC 4 cut(s) 236, 877, 1851, 1989
XspI CTAG 4 cut(s) 689, 737, 771, 2070
ZraI GACGTC 1 cut(s) 1581
Zsp2I ATGCAT 2 cut(s) 621, 1947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.