pycom03g03420

Chloride channel protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0008013
Physical Location & Seq
Forward (+)
2702108 .. 2707400
5293 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g03420.6

Sequence Viewer

Length: 2400 bp
ATGTCAGGCGAGGAATACAGCGATGAGACTCGTCTGCTGCGATCCTCCAACGACATTATCAAAGACGACAACGCCGGCCTTTCGGCAGAGAAAGACAGCGACTTGGAAGCTCAAGAGGGATTAATAAGCAGCAGGAGAAGCAGTATTAGTCCGACCGGTAGAAGAGGCGGAGTCGGAGGCTTCAAAGATCTGCTGATCAAGCATTTGGACGGCGTAGGAGGGCTTTCCGGCCGCCGTCTCAGTATCAAGCGCGGCCGAGACAATTCCCCAAATCACGGCGGCAGAGAGGTTAGAGAGCAGCGTTCCGCTGTAGATCACCACCACCATCATAATCAGCATCATCAGATTCAGCAGCAACATCATGAGTCTCCGGCCGGAAGCGACGGTAATGACGAACTTGCCGATAGTGCCCCACCGGAGTGGGCGTTGCTTCTCCTTGGTTGCCTTCTTGGCCTCGCCACAGGTCTCTTTGTTGCCGCATTTAACAAAGGGGTACATGTTATACATGAATGGGCCTGGGCTGGTACTCCAAGTGAAGGTGCTGCATGGCTCCGTCTGCAGAGACTAGGCGATACTTGGCATCGAATTCTCCTAATACCGGTCACGGGTGGGGTTATTGTTGGCATGATGCATGGTTTACTTGAAATATTGGACCAGATAAGGCAGTCCAGTTCCTCTCAAAGGCAAGGATTTGATTTACTTGCTGCAGTCTTTCCCTCAATAAAGGCTGTCCAAGCAGCTGTCACTTTAGGTACCGGTTGTTCTTTGGGTCCTGAAGGCCCTAGTGTAGATATTGGAAAATCTTGTGCCAATGGATTCTCATTAATGATGGAAAACAATAGAGAAAGGAAGATTGCCCTTGTTGCAGCAGGTGCAGCGGCTGGAATTTCTTCAGGTTTCAATGCGGCAGTTGCTGGTTGTTTCTTTGCTATTGAAACGGTGTTAAGGCCTCTTCGAGCAGAAAACTCACCTCCATTCACAACTGCAATGATTATTTTGGCTTCTGTTATATCATCCTCTGTATCTAATGTTTTACAAGGGACTCAATCAGCGTTTACAGTGCCTACATACGATCTAAAATCTGCTGCTGAACTTCCTCTATACCTGATATTGGGAATGCTATGTGGTGCTGTAAGTGTAGCCTTGACTCGATTGGTTGCTTGGTTCACAAGGTTCTTTGATTTTATCAAGGAAAGATTTGGTCTTCCAGCTGTTGCCTGCCCAGCTTTAGGTGGTTTAGGAGCTGGGATTATTGCTCTTAAGTATCCTGGAATTCTGTACTGGGGTTTTACAAATGTTGAAGAAATTTTGCATACTGGGAATGCTTCGGCTCCTGGAATCTGGCTGTTAACTCAGTTAGCAGCAGCTAAAGTTGTGGCAACAGCTTTATGCAAGGGTTCTGGGTTGGTAGGTGGCCTGTACGCACCAAGCTTGATGATTGGTGCTGCTGTTGGTGCTGTATTTGGGGGTTCGGCTGCAGAACTGATAAACTCAGCGATTCCAGGAAATGCTGCTGTTGCCCAGCCACAGGCTTATGCACTGGTTGGAATGGCTGCTACACTAGCTTCAGTTTGTTCAGTACCATTGACGTCAGTTCTGCTTCTGTTTGAGCTCACAAAAGATTACCGGATATTGCTCCCTCTCATGGGGGCTGTTGGATTGGCAATATGGGTGCCCTCTGTGGTAAACCAGGAAAAGGAGACTGAACCTTCTGAAACACGGAATTCAGCAAGAGTTTATTCTTATGTTTCAGCTGATGAAGAAAAAGACGAAGCTAATTGGAGACAACATGATGATGGAGATGATTTGGAACTCTCTGTTATTGGAAACTATTCTGACTCCGAATTAGTTAGTGAAGAATTGCTTCTGGAAGATCTAAAGGTTTCGCGAGCCATGTCAAAGAAATATGCGAAGGTTCCTGTGAGCTTGACTCTGAAAGACGCAATAAAATGCATGCATGAGAGTCACCAGAATTGTGTTCTGGTGGTTGATGATGAAGATTTTCTGGAAGGAATATTAACATATGGTGATGTTAGACGGTTTCAATCAAAGAAGTCTAGCGATGCATCAAAGAGCGAATCTAGATTTTTGGATGCAAATACATATCTTGTTTCATCTGTTTGTACTCGAGGAATTCGCTACAGAGGACGAGCGCGTGGTTTATTTACCTGTTATCCGGACACAGATTTAGCAATGGCCAAGGAGCTAATGGAGGCCAAGGATATAAGGCAGTTGCCTGTGGTTAAGCGTGTCAGAGAACCACCAAAAGAAATAAAGCGAAGAATTGTTGCTATTCTTCATTACGATTCAATCTTGAACTGCCTCAGAGAGGAGATAAATCACAGGAAAACAGTTCAACATAGAAAAGAGAATCTCCACGAGATTACCAATGGCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0009507 GO:0009536 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CBS Voltage_CLC

Protein Analysis

800

Amino Acids

86.24

Weight (kDa)

6.24

Isoelectric Point (pI)

44.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 860
AatII GACGTC 1 cut(s) 1592
Acc36I ACCTGC 1 cut(s) 860
Acc65I GGTACC 1 cut(s) 752
AccB1I GGYRCC 2 cut(s) 752, 1672
AccII CGCG 3 cut(s) 252, 1888, 2155
AccIII TCCGGA 1 cut(s) 2176
AciI CCGC 8 cut(s) 168, 232, 252, 279, 306, 477, 878, 905
AclWI GGATC 1 cut(s) 36
AcoI YGGCCR 4 cut(s) 229, 253, 372, 2196
AcsI RAATTY 6 cut(s) 585, 885, 1272, 1305, 1723, 2133
AcuI CTGAAG 3 cut(s) 795, 876, 1551
AcyI GRCGYC 1 cut(s) 1589
AfaI GTAC 7 cut(s) 495, 526, 754, 1280, 1421, 1581, 2125
AflII CTTAAG 1 cut(s) 1259
AflIII ACRYGT 1 cut(s) 496
AgeI ACCGGT 3 cut(s) 155, 598, 755
AgsI TTSAA 9 cut(s) 184, 644, 901, 935, 1301, 2045, 2310, 2317, 2357
AjnI CCWGG 5 cut(s) 515, 1267, 1333, 1501, 1689
AleI CACNNNNGTG 1 cut(s) 418
Alw21I GWGCWC 1 cut(s) 1614
Alw26I GTCTC 8 cut(s) 20, 242, 252, 372, 470, 556, 1694, 1777
AlwI GGATC 1 cut(s) 36
AlwNI CAGNNNCTG 2 cut(s) 881, 914
Ama87I CYCGRG 1 cut(s) 2127
Aor13HI TCCGGA 1 cut(s) 2176
ApoI RAATTY 6 cut(s) 585, 885, 1272, 1305, 1723, 2133
AseI ATTAAT 2 cut(s) 122, 824
AsiGI ACCGGT 3 cut(s) 155, 598, 755
Asp700I GAANNNNTTC 4 cut(s) 889, 1831, 1863, 2001
Asp718I GGTACC 1 cut(s) 752
AspLEI GCGC 2 cut(s) 252, 2155
AspS9I GGNCC 4 cut(s) 513, 652, 770, 779
AsuHPI GGTGA 4 cut(s) 308, 960, 1958, 2039
AvaI CYCGRG 1 cut(s) 2127
AvaII GGWCC 2 cut(s) 652, 770
BaeGI GKGCMC 2 cut(s) 412, 1677
BalI TGGCCA 1 cut(s) 2198
BanI GGYRCC 2 cut(s) 752, 1672
BanII GRGCYC 1 cut(s) 1614
BauI CACGAG 1 cut(s) 2378
BbsI GAAGAC 1 cut(s) 1196
Bbv12I GWGCWC 1 cut(s) 1614
BccI CCATC 3 cut(s) 333, 823, 1790
BceAI ACGGC 3 cut(s) 219, 226, 292
BciT130I CCWGG 5 cut(s) 517, 1269, 1335, 1503, 1691
BciVI GTATCC 1 cut(s) 1275
BclI TGATCA 1 cut(s) 195
BcoDI GTCTC 8 cut(s) 20, 242, 252, 372, 470, 556, 1694, 1777
BfaI CTAG 5 cut(s) 566, 783, 1562, 2058, 2082
BfmI CTRYAG 5 cut(s) 309, 557, 705, 1476, 2140
BfrI CTTAAG 1 cut(s) 1259
BfuAI ACCTGC 1 cut(s) 860
BfuI GTATCC 1 cut(s) 1275
BglI GCCNNNNNGGC 1 cut(s) 450
BglII AGATCT 2 cut(s) 187, 1873
Bme1390I CCNGG 5 cut(s) 517, 1269, 1335, 1503, 1691
Bme18I GGWCC 2 cut(s) 652, 770
BmeT110I CYCGRG 1 cut(s) 2127
BmgT120I GGNCC 4 cut(s) 513, 652, 770, 779
BmiI GGNNCC 6 cut(s) 551, 754, 771, 1332, 1674, 1917
BmrFI CCNGG 5 cut(s) 517, 1269, 1335, 1503, 1691
BmrI ACTGGG 2 cut(s) 1291, 1326
BmsI GCATC 6 cut(s) 346, 589, 618, 2053, 2075, 2083
BmuI ACTGGG 2 cut(s) 1291, 1326
BpiI GAAGAC 1 cut(s) 1196
BplI GAGNNNNNCTC 2 cut(s) 1915, 1947
BpuEI CTTGAG 1 cut(s) 96
BsaHI GRCGYC 1 cut(s) 1589
BsaI GGTCTC 1 cut(s) 470
BsaJI CCNNGG 4 cut(s) 436, 516, 2199, 2217
BsaWI WCCGGW 6 cut(s) 155, 415, 598, 755, 1626, 2176
BsaXI ACNNNNNCTCC 4 cut(s) 127, 157, 410, 440
Bse118I RCCGGY 4 cut(s) 74, 155, 598, 755
Bse1I ACTGG 4 cut(s) 669, 1286, 1321, 1545
Bse3DI GCAATG 2 cut(s) 993, 2199
BseAI TCCGGA 1 cut(s) 2176
BseBI CCWGG 5 cut(s) 517, 1269, 1335, 1503, 1691
BseDI CCNNGG 4 cut(s) 436, 516, 2199, 2217
BseGI GGATG 2 cut(s) 1013, 2098
BseMI GCAATG 2 cut(s) 993, 2199
BseMII CTCAG 4 cut(s) 253, 1367, 1506, 2338
BseNI ACTGG 4 cut(s) 669, 1286, 1321, 1545
BseRI GAGGAG 1 cut(s) 2345
BseSI GKGCMC 2 cut(s) 412, 1677
BseX3I CGGCCG 3 cut(s) 229, 253, 372
BseYI CCCAGC 3 cut(s) 1222, 1244, 1521
BsgI GTGCAG 1 cut(s) 894
Bsh1236I CGCG 3 cut(s) 252, 1888, 2155
Bsh1285I CGRYCG 4 cut(s) 156, 232, 256, 375
BshNI GGYRCC 2 cut(s) 752, 1672
BshTI ACCGGT 3 cut(s) 155, 598, 755
BsiEI CGRYCG 4 cut(s) 156, 232, 256, 375
BsiHKAI GWGCWC 1 cut(s) 1614
BsiHKCI CYCGRG 1 cut(s) 2127
BslFI GGGAC 1 cut(s) 1054
BsmAI GTCTC 8 cut(s) 20, 242, 252, 372, 470, 556, 1694, 1777
BsmBI CGTCTC 1 cut(s) 242
BsmFI GGGAC 1 cut(s) 1054
BsmI GAATGC 2 cut(s) 1122, 1327
Bso31I GGTCTC 1 cut(s) 470
BsoBI CYCGRG 1 cut(s) 2127
Bsp1286I GDGCHC 3 cut(s) 412, 1614, 1677
Bsp13I TCCGGA 1 cut(s) 2176
Bsp143I GATC 6 cut(s) 41, 187, 195, 313, 1072, 1873
Bsp68I TCGCGA 1 cut(s) 1888
BspACI CCGC 8 cut(s) 168, 232, 252, 279, 306, 477, 878, 905
BspCNI CTCAG 4 cut(s) 252, 1366, 1505, 2337
BspEI TCCGGA 1 cut(s) 2176
BspFNI CGCG 3 cut(s) 252, 1888, 2155
BspHI TCATGA 1 cut(s) 361
BspLI GGNNCC 6 cut(s) 551, 754, 771, 1332, 1674, 1917
BspMAI CTGCAG 3 cut(s) 561, 709, 1480
BspMI ACCTGC 1 cut(s) 860
BspPI GGATC 1 cut(s) 36
BspT107I GGYRCC 2 cut(s) 752, 1672
BspTI CTTAAG 1 cut(s) 1259
BspTNI GGTCTC 1 cut(s) 470
BsrDI GCAATG 2 cut(s) 993, 2199
BsrFI RCCGGY 4 cut(s) 74, 155, 598, 755
BsrI ACTGG 4 cut(s) 669, 1286, 1321, 1545
BssAI RCCGGY 4 cut(s) 74, 155, 598, 755
BssECI CCNNGG 4 cut(s) 436, 516, 2199, 2217
BssMI GATC 6 cut(s) 41, 187, 195, 313, 1072, 1873
BssNI GRCGYC 1 cut(s) 1589
BssSI CACGAG 1 cut(s) 2378
BssT1I CCWWGG 3 cut(s) 436, 2199, 2217
Bst2BI CACGAG 1 cut(s) 2378
Bst2UI CCWGG 5 cut(s) 517, 1269, 1335, 1503, 1691
Bst4CI ACNGT 5 cut(s) 386, 940, 1060, 2040, 2353
Bst6I CTCTTC 2 cut(s) 157, 957
BstACI GRCGYC 1 cut(s) 1589
BstAFI CTTAAG 1 cut(s) 1259
BstAPI GCANNNNNTGC 1 cut(s) 872
BstC8I GCNNGC 4 cut(s) 76, 1219, 1890, 1955
BstDEI CTNAG 4 cut(s) 239, 1353, 1492, 2324
BstENI CCTNNNNNAGG 2 cut(s) 679, 2327
BstF5I GGATG 2 cut(s) 1013, 2098
BstFNI CGCG 3 cut(s) 252, 1888, 2155
BstHHI GCGC 2 cut(s) 252, 2155
BstKTI GATC 6 cut(s) 44, 190, 198, 316, 1075, 1876
BstMAI GTCTC 8 cut(s) 20, 242, 252, 372, 470, 556, 1694, 1777
BstMBI GATC 6 cut(s) 41, 187, 195, 313, 1072, 1873
BstMCI CGRYCG 4 cut(s) 156, 232, 256, 375
BstNI CCWGG 5 cut(s) 517, 1269, 1335, 1503, 1691
BstNSI RCATGY 2 cut(s) 500, 1957
BstSCI CCNGG 5 cut(s) 515, 1267, 1333, 1501, 1689
BstSFI CTRYAG 5 cut(s) 309, 557, 705, 1476, 2140
BstSLI GKGCMC 2 cut(s) 412, 1677
BstUI CGCG 3 cut(s) 252, 1888, 2155
BstV2I GAAGAC 1 cut(s) 1196
BstX2I RGATCY 2 cut(s) 187, 1873
BstXI CCANNNNNNTGG 1 cut(s) 420
BstYI RGATCY 2 cut(s) 187, 1873
BstZI CGGCCG 3 cut(s) 229, 253, 372
BsuI GTATCC 1 cut(s) 1275
BtgZI GCGATG 2 cut(s) 36, 2076
BtsCI GGATG 2 cut(s) 1013, 2098
BtsIMutI CAGTG 2 cut(s) 1065, 1538
BtuMI TCGCGA 1 cut(s) 1888
BveI ACCTGC 1 cut(s) 860
Cac8I GCNNGC 4 cut(s) 76, 1219, 1890, 1955
CaiI CAGNNNCTG 2 cut(s) 881, 914
CciI TCATGA 1 cut(s) 361
CfoI GCGC 2 cut(s) 252, 2155
Cfr10I RCCGGY 4 cut(s) 74, 155, 598, 755
Cfr13I GGNCC 4 cut(s) 513, 652, 770, 779
CseI GACGC 1 cut(s) 1949
Csp6I GTAC 7 cut(s) 494, 525, 753, 1279, 1420, 1580, 2124
CspAI ACCGGT 3 cut(s) 155, 598, 755
CviQI GTAC 7 cut(s) 494, 525, 753, 1279, 1420, 1580, 2124
DdeI CTNAG 4 cut(s) 239, 1353, 1492, 2324
DpnI GATC 6 cut(s) 43, 189, 197, 315, 1074, 1875
DpnII GATC 6 cut(s) 41, 187, 195, 313, 1072, 1873
EaeI YGGCCR 4 cut(s) 229, 253, 372, 2196
EagI CGGCCG 3 cut(s) 229, 253, 372
Eam1104I CTCTTC 2 cut(s) 157, 957
EarI CTCTTC 2 cut(s) 157, 957
EciI GGCGGA 1 cut(s) 183
Ecl136II GAGCTC 1 cut(s) 1612
EclXI CGGCCG 3 cut(s) 229, 253, 372
Eco130I CCWWGG 3 cut(s) 436, 2199, 2217
Eco147I AGGCCT 1 cut(s) 949
Eco24I GRGCYC 1 cut(s) 1614
Eco31I GGTCTC 1 cut(s) 470
Eco47I GGWCC 2 cut(s) 652, 770
Eco52I CGGCCG 3 cut(s) 229, 253, 372
Eco53kI GAGCTC 1 cut(s) 1612
Eco57I CTGAAG 3 cut(s) 795, 876, 1551
Eco88I CYCGRG 1 cut(s) 2127
EcoICRI GAGCTC 1 cut(s) 1612
EcoNI CCTNNNNNAGG 2 cut(s) 679, 2327
EcoO109I RGGNCCY 2 cut(s) 770, 779
EcoRI GAATTC 4 cut(s) 585, 1272, 1723, 2133
EcoRII CCWGG 5 cut(s) 515, 1267, 1333, 1501, 1689
EcoT14I CCWWGG 3 cut(s) 436, 2199, 2217
EcoT22I ATGCAT 4 cut(s) 633, 1955, 1959, 2068
EcoT38I GRGCYC 1 cut(s) 1614
ErhI CCWWGG 3 cut(s) 436, 2199, 2217
Esp3I CGTCTC 1 cut(s) 242
FalI AAGNNNNNCTT 2 cut(s) 1848, 1880
FaqI GGGAC 1 cut(s) 1054
FauNDI CATATG 1 cut(s) 2023
FbaI TGATCA 1 cut(s) 195
FokI GGATG 2 cut(s) 1000, 2105
FriOI GRGCYC 1 cut(s) 1614
FspBI CTAG 5 cut(s) 566, 783, 1562, 2058, 2082
GlaI GCGC 2 cut(s) 251, 2154
GsaI CCCAGC 3 cut(s) 1226, 1248, 1525
HgaI GACGC 1 cut(s) 1949
HhaI GCGC 2 cut(s) 252, 2155
Hin1I GRCGYC 1 cut(s) 1589
Hin6I GCGC 2 cut(s) 250, 2153
HinP1I GCGC 2 cut(s) 250, 2153
HincII GTYRAC 1 cut(s) 1350
HindII GTYRAC 1 cut(s) 1350
HindIII AAGCTT 1 cut(s) 1429
HpaI GTTAAC 1 cut(s) 1350
HphI GGTGA 4 cut(s) 308, 960, 1958, 2039
Hpy166II GTNNAC 5 cut(s) 638, 1056, 1167, 1350, 1687
Hpy188I TCNGA 9 cut(s) 153, 176, 345, 1714, 1837, 1843, 1935, 2255, 2327
Hpy188III TCNNGA 9 cut(s) 113, 362, 773, 1868, 1887, 2006, 2082, 2177, 2314
Hpy8I GTNNAC 5 cut(s) 638, 1056, 1167, 1350, 1687
Hpy99I CGWCG 1 cut(s) 386
HpyAV CCTTC 6 cut(s) 455, 530, 770, 1719, 1906, 2003
HpyCH4III ACNGT 5 cut(s) 386, 940, 1060, 2040, 2353
HpyCH4IV ACGT 1 cut(s) 1589
HpyF3I CTNAG 4 cut(s) 239, 1353, 1492, 2324
HpySE526I ACGT 1 cut(s) 1589
Hsp92I GRCGYC 1 cut(s) 1589
HspAI GCGC 2 cut(s) 250, 2153
Kpn2I TCCGGA 1 cut(s) 2176
KpnI GGTACC 1 cut(s) 756
KroI GCCGGC 1 cut(s) 74
KroNI GCCGGC 1 cut(s) 76
Ksp22I TGATCA 1 cut(s) 195
KspAI GTTAAC 1 cut(s) 1350
Kzo9I GATC 6 cut(s) 41, 187, 195, 313, 1072, 1873
LmnI GCTCC 5 cut(s) 555, 1241, 1336, 1641, 2203
LweI GCATC 6 cut(s) 346, 589, 618, 2053, 2075, 2083
MaeI CTAG 5 cut(s) 566, 783, 1562, 2058, 2082
MaeII ACGT 1 cut(s) 1589
MaeIII GTNAC 3 cut(s) 601, 742, 1964
MalI GATC 6 cut(s) 43, 189, 197, 315, 1074, 1875
MboI GATC 6 cut(s) 41, 187, 195, 313, 1072, 1873
MflI RGATCY 2 cut(s) 187, 1873
MhlI GDGCHC 3 cut(s) 412, 1614, 1677
MlsI TGGCCA 1 cut(s) 2198
MluNI TGGCCA 1 cut(s) 2198
MlyI GAGTC 8 cut(s) 22, 180, 374, 1036, 1141, 1832, 1924, 1972
MmeI TCCRAC 5 cut(s) 72, 154, 176, 1525, 1636
Mox20I TGGCCA 1 cut(s) 2198
Mph1103I ATGCAT 4 cut(s) 633, 1955, 1959, 2068
MroI TCCGGA 1 cut(s) 2176
MroNI GCCGGC 1 cut(s) 74
MroXI GAANNNNTTC 4 cut(s) 889, 1831, 1863, 2001
MscI TGGCCA 1 cut(s) 2198
MseI TTAA 8 cut(s) 122, 483, 824, 944, 1260, 1349, 2018, 2244
MslI CAYNNNNRTG 2 cut(s) 418, 1794
Msp20I TGGCCA 1 cut(s) 2198
MspA1I CMGCKG 5 cut(s) 308, 740, 878, 1211, 1754
MspCI CTTAAG 1 cut(s) 1259
MspR9I CCNGG 5 cut(s) 517, 1269, 1335, 1503, 1691
Mva1269I GAATGC 2 cut(s) 1122, 1327
MvaI CCWGG 5 cut(s) 517, 1269, 1335, 1503, 1691
MvnI CGCG 3 cut(s) 252, 1888, 2155
NaeI GCCGGC 1 cut(s) 76
NdeI CATATG 1 cut(s) 2023
NdeII GATC 6 cut(s) 41, 187, 195, 313, 1072, 1873
NgoMIV GCCGGC 1 cut(s) 74
NlaIV GGNNCC 6 cut(s) 551, 754, 771, 1332, 1674, 1917
NmeAIII GCCGAG 1 cut(s) 281
NmuCI GTSAC 3 cut(s) 601, 742, 1964
NruI TCGCGA 1 cut(s) 1888
NsiI ATGCAT 4 cut(s) 633, 1955, 1959, 2068
NspI RCATGY 2 cut(s) 500, 1957
OliI CACNNNNGTG 1 cut(s) 418
PaeI GCATGC 1 cut(s) 1957
PaeR7I CTCGAG 1 cut(s) 2127
PagI TCATGA 1 cut(s) 361
PaqCI CACCTGC 1 cut(s) 860
PceI AGGCCT 1 cut(s) 949
PciI ACATGT 1 cut(s) 496
PcsI WCGNNNNNNNCGW 3 cut(s) 37, 390, 399
PctI GAATGC 2 cut(s) 1122, 1327
PdiI GCCGGC 1 cut(s) 76
PdmI GAANNNNTTC 4 cut(s) 889, 1831, 1863, 2001
PfeI GAWTC 7 cut(s) 346, 816, 1338, 1498, 2078, 2306, 2371
PfoI TCCNGGA 3 cut(s) 1267, 1333, 1501
PinAI ACCGGT 3 cut(s) 155, 598, 755
PleI GAGTC 8 cut(s) 22, 179, 373, 1036, 1141, 1832, 1924, 1971
PpsI GAGTC 8 cut(s) 22, 179, 373, 1036, 1141, 1832, 1924, 1971
PpuMI RGGWCCY 1 cut(s) 770
PscI ACATGT 1 cut(s) 496
PshBI ATTAAT 2 cut(s) 122, 824
Psp124BI GAGCTC 1 cut(s) 1614
Psp5II RGGWCCY 1 cut(s) 770
Psp6I CCWGG 5 cut(s) 515, 1267, 1333, 1501, 1689
PspFI CCCAGC 3 cut(s) 1222, 1244, 1521
PspGI CCWGG 5 cut(s) 515, 1267, 1333, 1501, 1689
PspN4I GGNNCC 6 cut(s) 551, 754, 771, 1332, 1674, 1917
PspPI GGNCC 4 cut(s) 513, 652, 770, 779
PspPPI RGGWCCY 1 cut(s) 770
PspXI VCTCGAGB 1 cut(s) 2127
PsrI GAACNNNNNNTAC 2 cut(s) 745, 777
PstI CTGCAG 3 cut(s) 561, 709, 1480
PstNI CAGNNNCTG 2 cut(s) 881, 914
PsuI RGATCY 2 cut(s) 187, 1873
PvuII CAGCTG 3 cut(s) 740, 1211, 1754
RruI TCGCGA 1 cut(s) 1888
RsaI GTAC 7 cut(s) 495, 526, 754, 1280, 1421, 1581, 2125
RsaNI GTAC 7 cut(s) 494, 525, 753, 1279, 1420, 1580, 2124
RseI CAYNNNNRTG 2 cut(s) 418, 1794
SacI GAGCTC 1 cut(s) 1614
SaqAI TTAA 8 cut(s) 122, 483, 824, 944, 1260, 1349, 2018, 2244
Sau3AI GATC 6 cut(s) 41, 187, 195, 313, 1072, 1873
Sau96I GGNCC 4 cut(s) 513, 652, 770, 779
SchI GAGTC 8 cut(s) 22, 180, 374, 1036, 1141, 1832, 1924, 1972
ScrFI CCNGG 5 cut(s) 517, 1269, 1335, 1503, 1691
SduI GDGCHC 3 cut(s) 412, 1614, 1677
SfaNI GCATC 6 cut(s) 346, 589, 618, 2053, 2075, 2083
SfcI CTRYAG 5 cut(s) 309, 557, 705, 1476, 2140
Sfr274I CTCGAG 1 cut(s) 2127
SinI GGWCC 2 cut(s) 652, 770
SlaI CTCGAG 1 cut(s) 2127
SmiMI CAYNNNNRTG 2 cut(s) 418, 1794
SmlI CTYRAG 3 cut(s) 111, 1259, 2127
SmoI CTYRAG 3 cut(s) 111, 1259, 2127
SphI GCATGC 1 cut(s) 1957
SseBI AGGCCT 1 cut(s) 949
SsiI CCGC 8 cut(s) 168, 232, 252, 279, 306, 477, 878, 905
SspI AATATT 2 cut(s) 648, 2016
SspMI CTAG 5 cut(s) 566, 783, 1562, 2058, 2082
SstI GAGCTC 1 cut(s) 1614
StuI AGGCCT 1 cut(s) 949
StyD4I CCNGG 5 cut(s) 515, 1267, 1333, 1501, 1689
StyI CCWWGG 3 cut(s) 436, 2199, 2217
TaaI ACNGT 5 cut(s) 386, 940, 1060, 2040, 2353
TaiI ACGT 1 cut(s) 1592
TaqI TCGA 4 cut(s) 583, 955, 1150, 2128
TatI WGTACW 2 cut(s) 1278, 2123
TauI GCSGC 6 cut(s) 234, 255, 282, 479, 881, 908
TfiI GAWTC 7 cut(s) 346, 816, 1338, 1498, 2078, 2306, 2371
Tru1I TTAA 8 cut(s) 122, 483, 824, 944, 1260, 1349, 2018, 2244
Tru9I TTAA 8 cut(s) 122, 483, 824, 944, 1260, 1349, 2018, 2244
TscAI CASTG 2 cut(s) 1065, 1545
TseFI GTSAC 3 cut(s) 601, 742, 1964
Tsp45I GTSAC 3 cut(s) 601, 742, 1964
TspDTI ATGAA 5 cut(s) 522, 1773, 2010, 2103, 2288
TspGWI ACGGA 2 cut(s) 542, 1735
TspRI CASTG 2 cut(s) 1065, 1545
Vha464I CTTAAG 1 cut(s) 1259
VpaK11BI GGWCC 2 cut(s) 652, 770
VspI ATTAAT 2 cut(s) 122, 824
XagI CCTNNNNNAGG 2 cut(s) 679, 2327
XapI RAATTY 6 cut(s) 585, 885, 1272, 1305, 1723, 2133
XbaI TCTAGA 1 cut(s) 2081
XceI RCATGY 2 cut(s) 500, 1957
XcmI CCANNNNNNNNNTGG 1 cut(s) 2206
XhoI CTCGAG 1 cut(s) 2127
XmnI GAANNNNTTC 4 cut(s) 889, 1831, 1863, 2001
XspI CTAG 5 cut(s) 566, 783, 1562, 2058, 2082
ZraI GACGTC 1 cut(s) 1590
Zsp2I ATGCAT 4 cut(s) 633, 1955, 1959, 2068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.