Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0008013

Physical Location & Seq

Genomic Coordinates Forward (+)

4109475 .. 4115442

Gene Structure 5968 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1047300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2391 bp

ATGTCAGGAGAGGAATACAGCGATGAGAATCATCTGCTGCGATCCTCCAACGACATTACCGACGACGAAAACGCCGGCGTTTCGGCAGAGAAAGACAACGACTTGGAAGCTCAGGAGGGATTAATAAGCAGCAGGAAAAGCAGTATTAGTCCCACCAGTAGACGAGGCGGAGTCGGAGGCTTCAAGGATCTGCTGATCAAGCATTTGGACGGCGGAGGTGGGCTTTCCGGCCGCCGTCTCAATGTCAAGCGCGGCCGAGATAATTCCCCAAATCACGGCGGCAGAGAGATTAGAGACCAGCAACGGCCCCCTGTAGATCACCACCACCATCATAATCAGCAGCATCATGAGTCTTCGGCTGGAATAGACGGTAATGATGAGCTTGCCGATAGTGCGCCGCCCGAATGGGCTTTGCTTCTCCTTGGTTGCCTTCTCGGCCTCGCCACTGGTCTCCTTGTTGCCGCATTTAACAAAGGGGTACATGTTATACATGAATGGGCCTGGGCTGGTACTCCAAGTGATGGTGCTGCATGGCTTCGTCTGCAGAGACTGGGTGATACTTGGCATCGAATTCTCTTAATACCCGTCACGGGTGGGGTTATTGTTGGCATGATGCATGGTTTACTTGAAATATTAGGCCAGATAAGGCAGTCCGGTTCCTCTCAAAGACAAGGATTTGATTTACTTGCTGCAGTCTTTCCCTCAATAAAGGCTGTCCAAGCAGCTGTCACTTTAGGTACTGGTTGTTCTTTGGGTCCTGAAGGCCCTAGTGTAGATATTGGAAAATCTTGTGCCAATGGAATCTCATTAATGATGGAGAACAACAGAGAAAGGAAGATTGCCCTTGTTGCAGCAGGTGCAGCAGCTGGAATTTCTTCAGGTTTCAACGCGGCAGTTGCTGGTTGTTTCTTCGCTATTGAAACGGTGTTAAGGCCTCTTCGAGCAGAAAACTCACCTCCATTTACAACTGCAATGATTATTTTGGCTTCTGTTATATCATCCACTGTATCTAATGTTTTACAAGGGACTCAGTCAGCATTTACAGTGCCTACATATGATTTAAAATCTGCTGCTGAACTTCCTCTATACCTGATATTGGGAATGCTATGTGGTGCTGTAAGTGTAGCCTTGACTCGATTGGTTGCTTGGTTCACAAAGTTCTTTGATTTCATCAAGGAAAGATTTGGTCTTCCTGATGTTGCCTGCCCAGCTTTAGGTGGTTTAGGTGCTGGGATTATCGCTCTTAAGTATCCTGGAATTCTGTATTGGGGATTTACAAATGTTGAAGAAATTTTGCACACTGGGAGGACTGCTTCAGCTCCTGGAATCTGGCTGTTAACTCAGTTAGCAGCAGCTAAAGTTGTGGCAACAGCTTTATGCAAGGGTTCTGGGCTGGTAGGTGGCCTATACGCACCAAGCTTGATGATTGGGGCTGCTGTTGGTGCTGTATTTGGGGGTTCAGCTGCAGAACTGATAAACTCAGCAATTCCAGGAAATGCTGCTGTTGCCCAGCCACAGGCTTATGCACTGGTTGGAATGGCTGCTACGCTAGCTTCAGTTTGTTCAGTACCATTGACATCAGTTCTGCTTCTGTTTGAGCTCACAAAAGACTACCGGATATTGCTCCCTCTCATGGGGGCTGTTGGATTGGCAATATGGGTGCCCACTGTGGTAAACCAGGCAAAGGAGACTGAACCATCTGAAACACAGAATTCAGCAAGAGTTTATTCTTATGTTTCGGCTGATGAAGAAAAAGACGAAGTTAGTTGGAGACAACGTGATGATGGAGATGACTTGGAACTCACTGTTGTTGGAAACAATTCTGACTCCGAATTAGTTAGTGAAGAATTGCTTCTGGAAGATCTAAAGGTTTCGCAAGCCATGTCAAAGAAATATGTGAAGGTTCCTGTGAGCTTGACTCTGAAAGAGGCAATAAAATGCATGCACGAGAGTCATCAGAATTGTGTGTTGGTGGTTGATGGTGAAGATTTTCTGGAGGGAGTATTAACATACGGTGATGTTAGACGATTTCAATCGAAGAATTCTAGTGATACATCAGAGAGCGATACTAGAGTTTTGGATGCAAATAAATGTCTTGTTTCATCTGTTTGCACTCGACGAGCTCTCTACAGTGGACGAGCGCGTGGTTTATTTACCTGTTATCCGGACACAGATTTAGCAATGGCCAAGGAGCTAATGGAGGCCAAGGATATAAGGCAGTTGCCTGTGGTTAAGCGTGTCAGAGAGCCACCAAAAGAAATAAAGCGAAGAATCGTTGCTATTCTTCATTACGATTCAATCTTGAACTGTCTCAGAGAGGAGATAAATCACAGGAAAACAGTTCATCAACATAGAATAGAGAATCTCGACGAGATTAACAATGGCCATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0009507 GO:0009536 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS Voltage_CLC

Protein Analysis

797

Amino Acids

85.48

Weight (kDa)

5.85

Isoelectric Point (pI)

43.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Voltage_CLC	PF00654	230 - 554	3.8e-68	Voltage gated chloride channel
CBS	PF00571	624 - 678	3e-08	CBS domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008013)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55620 AT1G55620
fragaria_vesca	FvH4_3g41470 FvH4_3g41470 FvH4_3g41470 FvH4_3g41470 FvH4_3g41470 FvH4_3g41470 FvH4_3g41470
malus_domestica	MD03G1044300.v1.1 MD11G1047300.v1.1
prunus_persica	Prupe.6G036200_v2.0.a1
pyrus_communis	pycom03g03420 pycom11g03830
rosa_chinensis	RchiOBHm_Chr5g0074431
rosa_laevigata	RLG00000036476
rosa_multiflora	Rmu_sc0000573.1_g000001 Rmu_sc0025084.1_g000002
rosa_roxburghii	Rroxscaffold_1G00007090
rosa_rugosa	Rorug05G0431800
rosa_samantha	Rh5BG509800 Rh5CG533600 Rh5DG522700
rosa_wichuraiana	Rw5G045340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	845
Acc36I	ACCTGC	1 cut(s)	845
AccB1I	GGYRCC	1 cut(s)	1660
AccB7I	CCANNNNNTGG	1 cut(s)	521
AccI	GTMKAC	1 cut(s)	160
AccII	CGCG	3 cut(s)	252, 890, 2143
AccIII	TCCGGA	1 cut(s)	2164
AciI	CCGC	8 cut(s)	168, 213, 232, 252, 279, 398, 462, 890
AclWI	GGATC	2 cut(s)	36, 195
AcoI	YGGCCR	4 cut(s)	229, 253, 2184, 2383
AcsI	RAATTY	6 cut(s)	570, 870, 1257, 1290, 1711, 2041
AcuI	CTGAAG	4 cut(s)	780, 861, 1299, 1539
AfaI	GTAC	4 cut(s)	480, 511, 739, 1569
AfiI	CCNNNNNNNGG	9 cut(s)	275, 521, 590, 1096, 1214, 1516, 1633, 1634, 1684
AflII	CTTAAG	1 cut(s)	1244
AflIII	ACRYGT	1 cut(s)	481
AgsI	TTSAA	8 cut(s)	184, 629, 886, 920, 1286, 2033, 2298, 2305
AjnI	CCWGG	5 cut(s)	500, 1252, 1321, 1489, 1677
AjuI	GAANNNNNNNTTGG	4 cut(s)	1249, 1281, 1952, 1984
Alw21I	GWGCWC	2 cut(s)	1602, 2125
Alw26I	GTCTC	7 cut(s)	242, 288, 455, 541, 1682, 1765, 2315
AlwI	GGATC	2 cut(s)	36, 195
AlwNI	CAGNNNCTG	4 cut(s)	550, 866, 899, 1322
Aor13HI	TCCGGA	1 cut(s)	2164
ApoI	RAATTY	6 cut(s)	570, 870, 1257, 1290, 1711, 2041
AseI	ATTAAT	2 cut(s)	122, 809
Asp700I	GAANNNNTTC	4 cut(s)	874, 1819, 1851, 1989
AspLEI	GCGC	3 cut(s)	252, 397, 2143
AspS9I	GGNCC	4 cut(s)	306, 498, 755, 764
AsuHPI	GGTGA	5 cut(s)	311, 566, 945, 1994, 2027
AsuNHI	GCTAGC	1 cut(s)	1549
AvaII	GGWCC	1 cut(s)	755
BaeGI	GKGCMC	1 cut(s)	1665
BaeI	ACNNNNGTAYC	2 cut(s)	2058, 2091
BalI	TGGCCA	2 cut(s)	2186, 2385
BanI	GGYRCC	1 cut(s)	1660
BanII	GRGCYC	2 cut(s)	1602, 2125
BauI	CACGAG	1 cut(s)	1946
BbsI	GAAGAC	2 cut(s)	345, 1181
Bbv12I	GWGCWC	2 cut(s)	1602, 2125
BccI	CCATC	6 cut(s)	336, 515, 808, 1705, 1778, 1973
BceAI	ACGGC	4 cut(s)	219, 226, 292, 320
BciT130I	CCWGG	5 cut(s)	502, 1254, 1323, 1491, 1679
BciVI	GTATCC	1 cut(s)	1260
BclI	TGATCA	1 cut(s)	195
BcoDI	GTCTC	7 cut(s)	242, 288, 455, 541, 1682, 1765, 2315
BfaI	CTAG	4 cut(s)	768, 1550, 2046, 2070
BfmI	CTRYAG	5 cut(s)	312, 542, 690, 1464, 2128
BfrI	CTTAAG	1 cut(s)	1244
BfuAI	ACCTGC	1 cut(s)	845
BfuI	GTATCC	1 cut(s)	1260
BglI	GCCNNNNNGGC	1 cut(s)	435
BglII	AGATCT	1 cut(s)	1861
Bme1390I	CCNGG	5 cut(s)	502, 1254, 1323, 1491, 1679
Bme18I	GGWCC	1 cut(s)	755
BmgT120I	GGNCC	4 cut(s)	306, 498, 755, 764
BmiI	GGNNCC	5 cut(s)	308, 658, 756, 1662, 1905
BmrFI	CCNGG	5 cut(s)	502, 1254, 1323, 1491, 1679
BmrI	ACTGGG	2 cut(s)	560, 1311
BmsI	GCATC	4 cut(s)	352, 574, 603, 2071
BmtI	GCTAGC	1 cut(s)	1553
BmuI	ACTGGG	2 cut(s)	560, 1311
BpiI	GAAGAC	2 cut(s)	345, 1181
BplI	GAGNNNNNCTC	2 cut(s)	1903, 1935
BpmI	CTGGAG	1 cut(s)	2015
Bpu10I	CCTNAGC	1 cut(s)	111
BsaBI	GATNNNNATC	2 cut(s)	27, 2032
BsaI	GGTCTC	2 cut(s)	288, 455
BsaJI	CCNNGG	4 cut(s)	421, 501, 2187, 2205
BsaWI	WCCGGW	3 cut(s)	653, 1614, 2164
BsaXI	ACNNNNNCTCC	2 cut(s)	1762, 1792
Bsc4I	CCNNNNNNNGG	9 cut(s)	275, 521, 590, 1096, 1214, 1516, 1633, 1634, 1684
Bse118I	RCCGGY	1 cut(s)	74
Bse1I	ACTGG	6 cut(s)	156, 451, 555, 745, 1306, 1533
Bse3DI	GCAATG	2 cut(s)	978, 2187
Bse8I	GATNNNNATC	2 cut(s)	27, 2032
BseAI	TCCGGA	1 cut(s)	2164
BseBI	CCWGG	5 cut(s)	502, 1254, 1323, 1491, 1679
BseDI	CCNNGG	4 cut(s)	421, 501, 2187, 2205
BseGI	GGATG	2 cut(s)	998, 2086
BseJI	GATNNNNATC	2 cut(s)	27, 2032
BseLI	CCNNNNNNNGG	9 cut(s)	275, 521, 590, 1096, 1214, 1516, 1633, 1634, 1684
BseMI	GCAATG	2 cut(s)	978, 2187
BseMII	CTCAG	5 cut(s)	125, 1043, 1355, 1494, 2326
BseNI	ACTGG	6 cut(s)	156, 451, 555, 745, 1306, 1533
BseRI	GAGGAG	1 cut(s)	2333
BseSI	GKGCMC	1 cut(s)	1665
BseX3I	CGGCCG	2 cut(s)	229, 253
BseYI	CCCAGC	3 cut(s)	1207, 1229, 1509
BsgI	GTGCAG	1 cut(s)	879
Bsh1236I	CGCG	3 cut(s)	252, 890, 2143
Bsh1285I	CGRYCG	2 cut(s)	232, 256
BshNI	GGYRCC	1 cut(s)	1660
BsiEI	CGRYCG	2 cut(s)	232, 256
BsiHKAI	GWGCWC	2 cut(s)	1602, 2125
BsiSI	CCGG	5 cut(s)	75, 228, 654, 1615, 2165
BslFI	GGGAC	2 cut(s)	135, 1039
BslI	CCNNNNNNNGG	9 cut(s)	275, 521, 590, 1096, 1214, 1516, 1633, 1634, 1684
BsmAI	GTCTC	7 cut(s)	242, 288, 455, 541, 1682, 1765, 2315
BsmBI	CGTCTC	1 cut(s)	242
BsmFI	GGGAC	2 cut(s)	135, 1039
BsmI	GAATGC	1 cut(s)	1107
Bso31I	GGTCTC	2 cut(s)	288, 455
Bsp1286I	GDGCHC	3 cut(s)	1602, 1665, 2125
Bsp13I	TCCGGA	1 cut(s)	2164
Bsp143I	GATC	5 cut(s)	41, 187, 195, 316, 1861
BspACI	CCGC	8 cut(s)	168, 213, 232, 252, 279, 398, 462, 890
BspCNI	CTCAG	5 cut(s)	124, 1042, 1354, 1493, 2325
BspEI	TCCGGA	1 cut(s)	2164
BspFNI	CGCG	3 cut(s)	252, 890, 2143
BspHI	TCATGA	1 cut(s)	346
BspLI	GGNNCC	5 cut(s)	308, 658, 756, 1662, 1905
BspMAI	CTGCAG	3 cut(s)	546, 694, 1468
BspMI	ACCTGC	1 cut(s)	845
BspOI	GCTAGC	1 cut(s)	1553
BspPI	GGATC	2 cut(s)	36, 195
BspT107I	GGYRCC	1 cut(s)	1660
BspTI	CTTAAG	1 cut(s)	1244
BspTNI	GGTCTC	2 cut(s)	288, 455
BsrDI	GCAATG	2 cut(s)	978, 2187
BsrFI	RCCGGY	1 cut(s)	74
BsrI	ACTGG	6 cut(s)	156, 451, 555, 745, 1306, 1533
BssAI	RCCGGY	1 cut(s)	74
BssECI	CCNNGG	4 cut(s)	421, 501, 2187, 2205
BssMI	GATC	5 cut(s)	41, 187, 195, 316, 1861
BssSI	CACGAG	1 cut(s)	1946
BssT1I	CCWWGG	3 cut(s)	421, 2187, 2205
Bst2BI	CACGAG	1 cut(s)	1946
Bst2UI	CCWGG	5 cut(s)	502, 1254, 1323, 1491, 1679
Bst6I	CTCTTC	1 cut(s)	942
BstAFI	CTTAAG	1 cut(s)	1244
BstAPI	GCANNNNNTGC	1 cut(s)	857
BstC8I	GCNNGC	6 cut(s)	76, 384, 1204, 1551, 1878, 1943
BstDEI	CTNAG	5 cut(s)	111, 1029, 1341, 1480, 2312
BstF5I	GGATG	2 cut(s)	998, 2086
BstFNI	CGCG	3 cut(s)	252, 890, 2143
BstHHI	GCGC	3 cut(s)	252, 397, 2143
BstKTI	GATC	5 cut(s)	44, 190, 198, 319, 1864
BstMAI	GTCTC	7 cut(s)	242, 288, 455, 541, 1682, 1765, 2315
BstMBI	GATC	5 cut(s)	41, 187, 195, 316, 1861
BstMCI	CGRYCG	2 cut(s)	232, 256
BstNI	CCWGG	5 cut(s)	502, 1254, 1323, 1491, 1679
BstNSI	RCATGY	2 cut(s)	485, 1945
BstSCI	CCNGG	5 cut(s)	500, 1252, 1321, 1489, 1677
BstSFI	CTRYAG	5 cut(s)	312, 542, 690, 1464, 2128
BstSLI	GKGCMC	1 cut(s)	1665
BstUI	CGCG	3 cut(s)	252, 890, 2143
BstV2I	GAAGAC	2 cut(s)	345, 1181
BstX2I	RGATCY	2 cut(s)	187, 1861
BstYI	RGATCY	2 cut(s)	187, 1861
BstZI	CGGCCG	2 cut(s)	229, 253
BsuI	GTATCC	1 cut(s)	1260
BtgZI	GCGATG	1 cut(s)	36
BtsCI	GGATG	2 cut(s)	998, 2086
BtsIMutI	CAGTG	8 cut(s)	444, 1002, 1050, 1299, 1526, 1665, 1803, 2137
BveI	ACCTGC	1 cut(s)	845
Cac8I	GCNNGC	6 cut(s)	76, 384, 1204, 1551, 1878, 1943
CaiI	CAGNNNCTG	4 cut(s)	550, 866, 899, 1322
CciI	TCATGA	1 cut(s)	346
CfoI	GCGC	3 cut(s)	252, 397, 2143
Cfr10I	RCCGGY	1 cut(s)	74
Cfr13I	GGNCC	4 cut(s)	306, 498, 755, 764
Csp6I	GTAC	4 cut(s)	479, 510, 738, 1568
CviAII	CATG	9 cut(s)	347, 482, 491, 531, 610, 617, 1633, 1882, 1942
CviQI	GTAC	4 cut(s)	479, 510, 738, 1568
DdeI	CTNAG	5 cut(s)	111, 1029, 1341, 1480, 2312
DpnI	GATC	5 cut(s)	43, 189, 197, 318, 1863
DpnII	GATC	5 cut(s)	41, 187, 195, 316, 1861
DraI	TTTAAA	1 cut(s)	1062
EaeI	YGGCCR	4 cut(s)	229, 253, 2184, 2383
EagI	CGGCCG	2 cut(s)	229, 253
Eam1104I	CTCTTC	1 cut(s)	942
EarI	CTCTTC	1 cut(s)	942
EciI	GGCGGA	2 cut(s)	183, 228
Ecl136II	GAGCTC	2 cut(s)	1600, 2123
EclXI	CGGCCG	2 cut(s)	229, 253
Eco130I	CCWWGG	3 cut(s)	421, 2187, 2205
Eco147I	AGGCCT	1 cut(s)	934
Eco24I	GRGCYC	2 cut(s)	1602, 2125
Eco31I	GGTCTC	2 cut(s)	288, 455
Eco47I	GGWCC	1 cut(s)	755
Eco52I	CGGCCG	2 cut(s)	229, 253
Eco53kI	GAGCTC	2 cut(s)	1600, 2123
Eco57I	CTGAAG	4 cut(s)	780, 861, 1299, 1539
EcoICRI	GAGCTC	2 cut(s)	1600, 2123
EcoO109I	RGGNCCY	2 cut(s)	755, 764
EcoRI	GAATTC	4 cut(s)	570, 1257, 1711, 2041
EcoRII	CCWGG	5 cut(s)	500, 1252, 1321, 1489, 1677
EcoT14I	CCWWGG	3 cut(s)	421, 2187, 2205
EcoT22I	ATGCAT	2 cut(s)	618, 1943
EcoT38I	GRGCYC	2 cut(s)	1602, 2125
ErhI	CCWWGG	3 cut(s)	421, 2187, 2205
Esp3I	CGTCTC	1 cut(s)	242
FaeI	CATG	9 cut(s)	350, 485, 494, 534, 613, 620, 1636, 1885, 1945
FalI	AAGNNNNNCTT	2 cut(s)	1836, 1868
FaqI	GGGAC	2 cut(s)	135, 1039
FatI	CATG	9 cut(s)	346, 481, 490, 530, 609, 616, 1632, 1881, 1941
FauNDI	CATATG	1 cut(s)	1054
FbaI	TGATCA	1 cut(s)	195
FblI	GTMKAC	1 cut(s)	160
FokI	GGATG	2 cut(s)	985, 2093
FriOI	GRGCYC	2 cut(s)	1602, 2125
FspBI	CTAG	4 cut(s)	768, 1550, 2046, 2070
GlaI	GCGC	3 cut(s)	251, 396, 2142
GsaI	CCCAGC	3 cut(s)	1211, 1233, 1513
GsuI	CTGGAG	1 cut(s)	2015
HapII	CCGG	5 cut(s)	75, 228, 654, 1615, 2165
HhaI	GCGC	3 cut(s)	252, 397, 2143
Hin1II	CATG	9 cut(s)	350, 485, 494, 534, 613, 620, 1636, 1885, 1945
Hin6I	GCGC	3 cut(s)	250, 395, 2141
HinP1I	GCGC	3 cut(s)	250, 395, 2141
HincII	GTYRAC	1 cut(s)	1338
HindII	GTYRAC	1 cut(s)	1338
HindIII	AAGCTT	1 cut(s)	1417
HpaI	GTTAAC	1 cut(s)	1338
HpaII	CCGG	5 cut(s)	75, 228, 654, 1615, 2165
HphI	GGTGA	5 cut(s)	311, 566, 945, 1994, 2027
Hpy166II	GTNNAC	6 cut(s)	161, 623, 1152, 1338, 1675, 2135
Hpy188I	TCNGA	9 cut(s)	176, 1702, 1825, 1831, 1923, 1959, 2059, 2243, 2315
Hpy8I	GTNNAC	6 cut(s)	161, 623, 1152, 1338, 1675, 2135
Hpy99I	CGWCG	4 cut(s)	65, 68, 2121, 2372
HpyAV	CCTTC	3 cut(s)	440, 755, 1894
HpyCH4IV	ACGT	1 cut(s)	1777
HpyF3I	CTNAG	5 cut(s)	111, 1029, 1341, 1480, 2312
HpySE526I	ACGT	1 cut(s)	1777
Hsp92II	CATG	9 cut(s)	350, 485, 494, 534, 613, 620, 1636, 1885, 1945
HspAI	GCGC	3 cut(s)	250, 395, 2141
Kpn2I	TCCGGA	1 cut(s)	2164
KroI	GCCGGC	1 cut(s)	74
KroNI	GCCGGC	1 cut(s)	76
Ksp22I	TGATCA	1 cut(s)	195
KspAI	GTTAAC	1 cut(s)	1338
Kzo9I	GATC	5 cut(s)	41, 187, 195, 316, 1861
LmnI	GCTCC	3 cut(s)	1324, 1629, 2191
LweI	GCATC	4 cut(s)	352, 574, 603, 2071
MaeI	CTAG	4 cut(s)	768, 1550, 2046, 2070
MaeII	ACGT	1 cut(s)	1777
MaeIII	GTNAC	2 cut(s)	586, 727
MalI	GATC	5 cut(s)	43, 189, 197, 318, 1863
MboI	GATC	5 cut(s)	41, 187, 195, 316, 1861
MflI	RGATCY	2 cut(s)	187, 1861
MhlI	GDGCHC	3 cut(s)	1602, 1665, 2125
MlsI	TGGCCA	2 cut(s)	2186, 2385
MluNI	TGGCCA	2 cut(s)	2186, 2385
MlyI	GAGTC	7 cut(s)	180, 359, 1021, 1126, 1820, 1912, 1960
MmeI	TCCRAC	6 cut(s)	72, 154, 1513, 1624, 1748, 1792
Mox20I	TGGCCA	2 cut(s)	2186, 2385
Mph1103I	ATGCAT	2 cut(s)	618, 1943
MreI	CGCCGGCG	1 cut(s)	74
MroI	TCCGGA	1 cut(s)	2164
MroNI	GCCGGC	1 cut(s)	74
MroXI	GAANNNNTTC	4 cut(s)	874, 1819, 1851, 1989
MscI	TGGCCA	2 cut(s)	2186, 2385
Msp20I	TGGCCA	2 cut(s)	2186, 2385
MspA1I	CMGCKG	3 cut(s)	725, 866, 1463
MspCI	CTTAAG	1 cut(s)	1244
MspI	CCGG	5 cut(s)	75, 228, 654, 1615, 2165
MspR9I	CCNGG	5 cut(s)	502, 1254, 1323, 1491, 1679
Mva1269I	GAATGC	1 cut(s)	1107
MvaI	CCWGG	5 cut(s)	502, 1254, 1323, 1491, 1679
MvnI	CGCG	3 cut(s)	252, 890, 2143
NaeI	GCCGGC	1 cut(s)	76
NdeI	CATATG	1 cut(s)	1054
NdeII	GATC	5 cut(s)	41, 187, 195, 316, 1861
NgoMIV	GCCGGC	1 cut(s)	74
NheI	GCTAGC	1 cut(s)	1549
NlaIII	CATG	9 cut(s)	350, 485, 494, 534, 613, 620, 1636, 1885, 1945
NlaIV	GGNNCC	5 cut(s)	308, 658, 756, 1662, 1905
NmeAIII	GCCGAG	2 cut(s)	281, 414
NmuCI	GTSAC	2 cut(s)	586, 727
NsiI	ATGCAT	2 cut(s)	618, 1943
NspI	RCATGY	2 cut(s)	485, 1945
PaeI	GCATGC	1 cut(s)	1945
PagI	TCATGA	1 cut(s)	346
PaqCI	CACCTGC	1 cut(s)	845
PceI	AGGCCT	1 cut(s)	934
PciI	ACATGT	1 cut(s)	481
PcsI	WCGNNNNNNNCGW	1 cut(s)	57
PctI	GAATGC	1 cut(s)	1107
PdiI	GCCGGC	1 cut(s)	76
PdmI	GAANNNNTTC	4 cut(s)	874, 1819, 1851, 1989
PfeI	GAWTC	6 cut(s)	28, 801, 1326, 2271, 2294, 2362
PflFI	GACNNNGTC	1 cut(s)	1030
PflMI	CCANNNNNTGG	1 cut(s)	521
PfoI	TCCNGGA	3 cut(s)	1252, 1321, 1489
PleI	GAGTC	7 cut(s)	179, 358, 1021, 1126, 1820, 1912, 1959
PpsI	GAGTC	7 cut(s)	179, 358, 1021, 1126, 1820, 1912, 1959
PpuMI	RGGWCCY	1 cut(s)	755
PscI	ACATGT	1 cut(s)	481
PshBI	ATTAAT	2 cut(s)	122, 809
Psp124BI	GAGCTC	2 cut(s)	1602, 2125
Psp5II	RGGWCCY	1 cut(s)	755
Psp6I	CCWGG	5 cut(s)	500, 1252, 1321, 1489, 1677
PspFI	CCCAGC	3 cut(s)	1207, 1229, 1509
PspGI	CCWGG	5 cut(s)	500, 1252, 1321, 1489, 1677
PspN4I	GGNNCC	5 cut(s)	308, 658, 756, 1662, 1905
PspPI	GGNCC	4 cut(s)	306, 498, 755, 764
PspPPI	RGGWCCY	1 cut(s)	755
PsrI	GAACNNNNNNTAC	2 cut(s)	730, 762
PstI	CTGCAG	3 cut(s)	546, 694, 1468
PstNI	CAGNNNCTG	4 cut(s)	550, 866, 899, 1322
PsuI	RGATCY	2 cut(s)	187, 1861
PsyI	GACNNNGTC	1 cut(s)	1030
PvuII	CAGCTG	3 cut(s)	725, 866, 1463
RsaI	GTAC	4 cut(s)	480, 511, 739, 1569
RsaNI	GTAC	4 cut(s)	479, 510, 738, 1568
SacI	GAGCTC	2 cut(s)	1602, 2125
Sau3AI	GATC	5 cut(s)	41, 187, 195, 316, 1861
Sau96I	GGNCC	4 cut(s)	306, 498, 755, 764
SchI	GAGTC	7 cut(s)	180, 359, 1021, 1126, 1820, 1912, 1960
ScrFI	CCNGG	5 cut(s)	502, 1254, 1323, 1491, 1679
SduI	GDGCHC	3 cut(s)	1602, 1665, 2125
SfaNI	GCATC	4 cut(s)	352, 574, 603, 2071
SfcI	CTRYAG	5 cut(s)	312, 542, 690, 1464, 2128
SgrAI	CRCCGGYG	1 cut(s)	74
SinI	GGWCC	1 cut(s)	755
SmlI	CTYRAG	1 cut(s)	1244
SmoI	CTYRAG	1 cut(s)	1244
SphI	GCATGC	1 cut(s)	1945
SseBI	AGGCCT	1 cut(s)	934
SsiI	CCGC	8 cut(s)	168, 213, 232, 252, 279, 398, 462, 890
SspI	AATATT	1 cut(s)	633
SspMI	CTAG	4 cut(s)	768, 1550, 2046, 2070
SstI	GAGCTC	2 cut(s)	1602, 2125
StuI	AGGCCT	1 cut(s)	934
StyD4I	CCNGG	5 cut(s)	500, 1252, 1321, 1489, 1677
StyI	CCWWGG	3 cut(s)	421, 2187, 2205
TaiI	ACGT	1 cut(s)	1780
TaqI	TCGA	6 cut(s)	568, 940, 1135, 2036, 2116, 2367
TauI	GCSGC	6 cut(s)	234, 255, 282, 400, 464, 893
TfiI	GAWTC	6 cut(s)	28, 801, 1326, 2271, 2294, 2362
TscAI	CASTG	8 cut(s)	451, 1009, 1050, 1306, 1533, 1672, 1810, 2137
TseFI	GTSAC	2 cut(s)	586, 727
Tsp45I	GTSAC	2 cut(s)	586, 727
TspDTI	ATGAA	6 cut(s)	507, 1159, 1761, 2091, 2276, 2333
TspRI	CASTG	8 cut(s)	451, 1009, 1050, 1306, 1533, 1672, 1810, 2137
Tth111I	GACNNNGTC	1 cut(s)	1030
Van91I	CCANNNNNTGG	1 cut(s)	521
Vha464I	CTTAAG	1 cut(s)	1244
VpaK11BI	GGWCC	1 cut(s)	755
VspI	ATTAAT	2 cut(s)	122, 809
XapI	RAATTY	6 cut(s)	570, 870, 1257, 1290, 1711, 2041
XceI	RCATGY	2 cut(s)	485, 1945
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2194
XmiI	GTMKAC	1 cut(s)	160
XmnI	GAANNNNTTC	4 cut(s)	874, 1819, 1851, 1989
XspI	CTAG	4 cut(s)	768, 1550, 2046, 2070
Zsp2I	ATGCAT	2 cut(s)	618, 1943

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.