Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0008013

Physical Location & Seq

Genomic Coordinates Reverse (-)

79261590 .. 79266716

Gene Structure 5127 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G045340.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2382 bp

ATGTCAGGAGAGGAGTACAGCGATGAGACTCATCTGCTGAGATCCTCGAACGACATTAACAAAGACGAGAACGGCGCCGTTTCGCCGGAGGACGACAACGACGGCGATGCTGACTTGGAAGCGCAGAGCCGGAAAAGTAGTATCAGTCCGAGCAGTAGAGTCGGTGCTTTTAAGGATCTGCTGCTGAAGCATTTGGACGGCGTAGGAGGGCTTTCCGGTCGCCGGAACAGTTTCAAGAGGCTCGATGGGGATTGGGATAGGGATAGGGGTAGGGAGAGTAATCGGAGAGAAAGTAGAGAGCGGCGATCGCCTGTAGATCAGCTGCGTCATAGTAGTAATCATGACCCGCCGGCCGGAATCGATGGGAACGATGAACTGGCCGATAGTGCTCCGCCGGAGTGGGCTTTGCTTCTCCTCGGTTGCATTCTCGGCCTCGCCACCGGTCTCTTTGTTGCCGCGTTTAACAAAGGAGTACATGTTATACACGAATGGGCCTGGGCTGGTACTCCAACTGAAGGTGCTGCATGGCTCCGTCTGCAGAGACTGGGCGATACTTGGCATCGAATACTTTTAGTACCCGTCACAGGTGGGGTTATTGTTGGCATGATGCATGGTTTACTTGAAATATTGGATCAAATAAGACAGTTAAGTTCCTCCCAAGGGAAAGGATTTGATTTACTAGCTGGAGTCTTTCCCACAATAAAGGCTGTCCAGGCAGCTATCACACTAGGTACTGGTTGTTCTTTAGGTCCTGAAGGCCCTAGTGTAGATATTGGAAAATCATGTGCCAATGGGTTCTCATTAATGATGGAAAACAACAGAGAAAGGAAGATTGCCCTCATTGCAGCAGGTGCTGCTGCTGGAATTTCTTCAGGATTTAATGCGGCAGTTGCTGGTTGTTTCTTTGCTATTGAAACGGTGCTAAGGCCACTTCGAGCAGAAAACTCTCCTCCTTTTACTACCGCAATGATTATATTGGCTTCTGTTATATCGTCCACAGTATCTAATGTTTTACTTGGGACGCAATCAGCCTTTACAGTGCCTGTTTACGATTTGAAATCAGCTGCTGAATTGCCCCTCTACCTTATATTGGGCATGCTATGTGGTGCTGTGAGTGTAGTCTTCAATCGCTTGGTTGCTTGGTTCACAAAGTTTTTTGATTATATCAAGGAAAGATTTGGCCTTCCTGCAGTTGCATGCCCTGCTTTAGGTGGTTTAGGAGCTGGGTTGATTGCTCTCAAGTATCCAGGAATTCTGTACTGGGGTTTCACAAATGTTGAAGAAATCCTGCATACTGGAAAGACTGCTTCCGCCCCTGGAATCTGGCTGTTAACTCAATTATCAGCAGCCAAAGTTGTAGCAACAGCTTTATGCAAGGGTTCTGGGCTTGTAGGTGGACTTTATGCACCAAGCTTGATGATTGGGGCTGCTGTTGGCGCTGTATTTGGGGGTTCAGCTGCAGAATTGATTAATTCAGCAATTCCAGGAAATGCTGCCGTTGCTCAGCCACAGGCATATGCACTGGTGGGAATGGCTGCTATGCTGGCTTCAGTTTGTTCGGTGCCATTGACGTCAGTTCTCCTTCTGTTTGAGCTGACAAAAGATTACCGAATATTGCTTCCTCTCATGGGGGCGGTTGGATTGGCAATTTGGGTTCCCTCTGTGGTAAACCAGGGAAAGGAGACTGAAGCATCTGATACACGGAATTCTGCGAGAGGTTACTCTTCTGTTTCAGCTGCTGAAGAGAAAGATGAAGTTATTTGGAGACAACATGATAATGGAGATGATATGGAACTGTCTACCATGGGGAATACTTCTGATTCTGAATTAACTGAAGAAATGCTTCTGGAGAATCTAAAGGTTTCGCGGGCCATGTCAAAGAATTATGTGAAGGTTTCTCTGACCATAACTATTGAAGAGGCAATAAAATCCATGCATGACAAGCATCAGAATTGTGTGTTAATTGTTGATGATGAAGATTTTCTGGAGGGAATATTGACATATGGTGATGTTAGACGGTATCAATCAAGGAAGTCTTCTGATACGTTGAAGATCGATTCTAGTTTTCTGGACGAAAACACCTGTCTTGTTTCCGCTGTATGTACTCGAGAAATCAGCTTTCATGGGCGAACCCGAGGTCTATTAACCTGTTATCCGGACACGGGTTTATTAATGGCCAAGGAGCTAATGGAGGCAAAAGATATCAAGCAGTTGCCTGTGGTTAAGCGTGGCAGACAACCACCAAAAGAAACGAGGCGAAGACTTGTTGCTATTCTTCATTACGATTCAATCTTGATGTGTCTCAGGGAGGAGATAAATCACAGGAAATCAATTCATCAACATAGAAAAGAGAATCTTGACGAAATAACAAATGGCCACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0009507 GO:0009536 GO:0012505 GO:0016020 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CBS Voltage_CLC

Protein Analysis

793

Amino Acids

85.63

Weight (kDa)

5.78

Isoelectric Point (pI)

42.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Voltage_CLC	PF00654	222 - 552	1.1e-70	Voltage gated chloride channel
CBS	PF00571	621 - 675	7.1e-09	CBS domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008013)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55620 AT1G55620
fragaria_vesca	FvH4_3g41470 FvH4_3g41470 FvH4_3g41470 FvH4_3g41470 FvH4_3g41470 FvH4_3g41470 FvH4_3g41470
malus_domestica	MD03G1044300.v1.1 MD11G1047300.v1.1
prunus_persica	Prupe.6G036200_v2.0.a1
pyrus_communis	pycom03g03420 pycom11g03830
rosa_chinensis	RchiOBHm_Chr5g0074431
rosa_laevigata	RLG00000036476
rosa_multiflora	Rmu_sc0000573.1_g000001 Rmu_sc0025084.1_g000002
rosa_roxburghii	Rroxscaffold_1G00007090
rosa_rugosa	Rorug05G0431800
rosa_samantha	Rh5BG509800 Rh5CG533600 Rh5DG522700
rosa_wichuraiana	Rw5G045340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	839
AatII	GACGTC	1 cut(s)	1574
Acc36I	ACCTGC	1 cut(s)	839
AccB1I	GGYRCC	2 cut(s)	74, 1561
AccBSI	CCGCTC	1 cut(s)	301
AccI	GTMKAC	1 cut(s)	1799
AccII	CGCG	2 cut(s)	458, 1867
AccIII	TCCGGA	1 cut(s)	2155
AclWI	GGATC	3 cut(s)	36, 183, 639
AcoI	YGGCCR	4 cut(s)	351, 378, 2175, 2374
AcsI	RAATTY	3 cut(s)	864, 1251, 1705
AcuI	CTGAAG	8 cut(s)	206, 534, 774, 855, 1533, 1707, 1761, 1854
AcyI	GRCGYC	2 cut(s)	75, 1571
AfaI	GTAC	7 cut(s)	17, 474, 505, 576, 733, 1259, 2104
AflIII	ACRYGT	1 cut(s)	475
AgeI	ACCGGT	1 cut(s)	440
AgsI	TTSAA	9 cut(s)	235, 623, 914, 1057, 1126, 1280, 1916, 2050, 2289
AjnI	CCWGG	6 cut(s)	494, 711, 1246, 1315, 1483, 1671
Alw21I	GWGCWC	1 cut(s)	391
Alw26I	GTCTC	6 cut(s)	20, 449, 535, 1676, 1759, 2306
AlwI	GGATC	3 cut(s)	36, 183, 639
AlwNI	CAGNNNCTG	6 cut(s)	544, 854, 893, 1043, 1067, 1739
Ama87I	CYCGRG	2 cut(s)	2106, 2133
Aor13HI	TCCGGA	1 cut(s)	2155
ApoI	RAATTY	3 cut(s)	864, 1251, 1705
ArsI	GACNNNNNNTTYG	2 cut(s)	2122, 2154
AseI	ATTAAT	3 cut(s)	803, 1470, 2171
AsiGI	ACCGGT	1 cut(s)	440
AsiSI	GCGATCGC	1 cut(s)	308
Asp700I	GAANNNNTTC	4 cut(s)	230, 868, 1842, 1980
AspLEI	GCGC	3 cut(s)	77, 124, 1439
AspS9I	GGNCC	4 cut(s)	492, 749, 758, 1869
AsuHPI	GGTGA	1 cut(s)	2018
AvaI	CYCGRG	2 cut(s)	2106, 2133
AvaII	GGWCC	1 cut(s)	749
BalI	TGGCCA	2 cut(s)	2177, 2376
BanI	GGYRCC	2 cut(s)	74, 1561
BbsI	GAAGAC	3 cut(s)	1114, 2028, 2266
Bbv12I	GWGCWC	1 cut(s)	391
BccI	CCATC	3 cut(s)	239, 356, 802
BceAI	ACGGC	5 cut(s)	62, 88, 118, 214, 1481
BcgI	CGANNNNNNTGC	4 cut(s)	89, 123, 142, 176
BciT130I	CCWGG	6 cut(s)	496, 713, 1248, 1317, 1485, 1673
BciVI	GTATCC	1 cut(s)	1254
BcoDI	GTCTC	6 cut(s)	20, 449, 535, 1676, 1759, 2306
BfaI	CTAG	4 cut(s)	680, 728, 762, 2061
BfmI	CTRYAG	4 cut(s)	312, 536, 1188, 1458
BfoI	RGCGCY	2 cut(s)	78, 1440
BfuAI	ACCTGC	1 cut(s)	839
BfuI	GTATCC	1 cut(s)	1254
BlpI	GCTNAGC	1 cut(s)	1503
Bme1390I	CCNGG	6 cut(s)	496, 713, 1248, 1317, 1485, 1673
Bme18I	GGWCC	1 cut(s)	749
BmeT110I	CYCGRG	2 cut(s)	2106, 2133
BmgT120I	GGNCC	4 cut(s)	492, 749, 758, 1869
BmiI	GGNNCC	4 cut(s)	76, 530, 1563, 1656
BmrFI	CCNGG	6 cut(s)	496, 713, 1248, 1317, 1485, 1673
BmrI	ACTGGG	2 cut(s)	554, 1270
BmsI	GCATC	5 cut(s)	97, 568, 597, 1700, 1954
BmuI	ACTGGG	2 cut(s)	554, 1270
BpiI	GAAGAC	3 cut(s)	1114, 2028, 2266
BpmI	CTGGAG	3 cut(s)	705, 1868, 2006
Bpu10I	CCTNAGC	1 cut(s)	923
Bpu1102I	GCTNAGC	1 cut(s)	1503
BpuEI	CTTGAG	1 cut(s)	1223
Bsa29I	ATCGAT	2 cut(s)	360, 2055
BsaHI	GRCGYC	2 cut(s)	75, 1571
BsaI	GGTCTC	1 cut(s)	449
BsaJI	CCNNGG	8 cut(s)	415, 495, 658, 1315, 1672, 1803, 2134, 2178
BsaWI	WCCGGW	3 cut(s)	215, 440, 2155
BsaXI	ACNNNNNCTCC	4 cut(s)	277, 307, 462, 492
Bse118I	RCCGGY	2 cut(s)	349, 440
Bse1I	ACTGG	6 cut(s)	381, 549, 739, 1265, 1300, 1527
Bse3DI	GCAATG	2 cut(s)	840, 972
BseAI	TCCGGA	1 cut(s)	2155
BseBI	CCWGG	6 cut(s)	496, 713, 1248, 1317, 1485, 1673
BseCI	ATCGAT	2 cut(s)	360, 2055
BseDI	CCNNGG	8 cut(s)	415, 495, 658, 1315, 1672, 1803, 2134, 2178
BseMI	GCAATG	2 cut(s)	840, 972
BseMII	CTCAG	3 cut(s)	29, 1517, 2317
BseNI	ACTGG	6 cut(s)	381, 549, 739, 1265, 1300, 1527
BseRI	GAGGAG	4 cut(s)	26, 404, 939, 2324
BseX3I	CGGCCG	1 cut(s)	351
BseYI	CCCAGC	1 cut(s)	1223
Bsh1236I	CGCG	2 cut(s)	458, 1867
Bsh1285I	CGRYCG	3 cut(s)	220, 308, 354
BshNI	GGYRCC	2 cut(s)	74, 1561
BshTI	ACCGGT	1 cut(s)	440
BshVI	ATCGAT	2 cut(s)	360, 2055
BsiEI	CGRYCG	3 cut(s)	220, 308, 354
BsiHKAI	GWGCWC	1 cut(s)	391
BsiHKCI	CYCGRG	2 cut(s)	2106, 2133
BsiSI	CCGG	9 cut(s)	86, 130, 216, 223, 350, 354, 395, 441, 2156
BslFI	GGGAC	1 cut(s)	1033
BsmAI	GTCTC	6 cut(s)	20, 449, 535, 1676, 1759, 2306
BsmFI	GGGAC	1 cut(s)	1033
BsmI	GAATGC	1 cut(s)	423
Bso31I	GGTCTC	1 cut(s)	449
BsoBI	CYCGRG	2 cut(s)	2106, 2133
Bsp1286I	GDGCHC	1 cut(s)	391
Bsp13I	TCCGGA	1 cut(s)	2155
Bsp143I	GATC	6 cut(s)	41, 175, 305, 316, 631, 2052
Bsp1720I	GCTNAGC	1 cut(s)	1503
Bsp19I	CCATGG	1 cut(s)	1803
BspCNI	CTCAG	3 cut(s)	30, 1516, 2316
BspDI	ATCGAT	2 cut(s)	360, 2055
BspEI	TCCGGA	1 cut(s)	2155
BspFNI	CGCG	2 cut(s)	458, 1867
BspHI	TCATGA	1 cut(s)	340
BspLI	GGNNCC	4 cut(s)	76, 530, 1563, 1656
BspMAI	CTGCAG	3 cut(s)	540, 1192, 1462
BspMI	ACCTGC	1 cut(s)	839
BspPI	GGATC	3 cut(s)	36, 183, 639
BspT107I	GGYRCC	2 cut(s)	74, 1561
BspTNI	GGTCTC	1 cut(s)	449
BsrBI	CCGCTC	1 cut(s)	301
BsrDI	GCAATG	2 cut(s)	840, 972
BsrFI	RCCGGY	2 cut(s)	349, 440
BsrI	ACTGG	6 cut(s)	381, 549, 739, 1265, 1300, 1527
BssAI	RCCGGY	2 cut(s)	349, 440
BssECI	CCNNGG	8 cut(s)	415, 495, 658, 1315, 1672, 1803, 2134, 2178
BssMI	GATC	6 cut(s)	41, 175, 305, 316, 631, 2052
BssNI	GRCGYC	2 cut(s)	75, 1571
BssT1I	CCWWGG	3 cut(s)	658, 1803, 2178
Bst2UI	CCWGG	6 cut(s)	496, 713, 1248, 1317, 1485, 1673
Bst4CI	ACNGT	7 cut(s)	230, 645, 919, 1000, 1039, 1797, 2019
Bst6I	CTCTTC	3 cut(s)	1729, 1737, 1911
BstACI	GRCGYC	2 cut(s)	75, 1571
BstAPI	GCANNNNNTGC	3 cut(s)	851, 854, 1202
BstC8I	GCNNGC	5 cut(s)	351, 1097, 1198, 1545, 1869
BstDEI	CTNAG	4 cut(s)	38, 923, 1503, 2303
BstDSI	CCRYGG	1 cut(s)	1803
BstENI	CCTNNNNNAGG	1 cut(s)	1206
BstFNI	CGCG	2 cut(s)	458, 1867
BstH2I	RGCGCY	2 cut(s)	78, 1440
BstHHI	GCGC	3 cut(s)	77, 124, 1439
BstKTI	GATC	6 cut(s)	44, 178, 308, 319, 634, 2055
BstMAI	GTCTC	6 cut(s)	20, 449, 535, 1676, 1759, 2306
BstMBI	GATC	6 cut(s)	41, 175, 305, 316, 631, 2052
BstMCI	CGRYCG	3 cut(s)	220, 308, 354
BstNI	CCWGG	6 cut(s)	496, 713, 1248, 1317, 1485, 1673
BstNSI	RCATGY	3 cut(s)	479, 1099, 1200
BstSCI	CCNGG	6 cut(s)	494, 711, 1246, 1315, 1483, 1671
BstSFI	CTRYAG	4 cut(s)	312, 536, 1188, 1458
BstUI	CGCG	2 cut(s)	458, 1867
BstV2I	GAAGAC	3 cut(s)	1114, 2028, 2266
BstX2I	RGATCY	2 cut(s)	41, 175
BstYI	RGATCY	2 cut(s)	41, 175
BstZI	CGGCCG	1 cut(s)	351
Bsu15I	ATCGAT	2 cut(s)	360, 2055
BsuI	GTATCC	1 cut(s)	1254
BsuTUI	ATCGAT	2 cut(s)	360, 2055
BtgI	CCRYGG	1 cut(s)	1803
BtgZI	GCGATG	2 cut(s)	36, 120
BtsIMutI	CAGTG	2 cut(s)	1044, 1520
BveI	ACCTGC	1 cut(s)	839
Cac8I	GCNNGC	5 cut(s)	351, 1097, 1198, 1545, 1869
CaiI	CAGNNNCTG	6 cut(s)	544, 854, 893, 1043, 1067, 1739
CciI	TCATGA	1 cut(s)	340
CfoI	GCGC	3 cut(s)	77, 124, 1439
Cfr10I	RCCGGY	2 cut(s)	349, 440
Cfr13I	GGNCC	4 cut(s)	492, 749, 758, 1869
ClaI	ATCGAT	2 cut(s)	360, 2055
CseI	GACGC	2 cut(s)	314, 1030
Csp6I	GTAC	7 cut(s)	16, 473, 504, 575, 732, 1258, 2103
CspAI	ACCGGT	1 cut(s)	440
CviQI	GTAC	7 cut(s)	16, 473, 504, 575, 732, 1258, 2103
DdeI	CTNAG	4 cut(s)	38, 923, 1503, 2303
DinI	GGCGCC	1 cut(s)	76
DpnI	GATC	6 cut(s)	43, 177, 307, 318, 633, 2054
DpnII	GATC	6 cut(s)	41, 175, 305, 316, 631, 2052
EaeI	YGGCCR	4 cut(s)	351, 378, 2175, 2374
EagI	CGGCCG	1 cut(s)	351
Eam1104I	CTCTTC	3 cut(s)	1729, 1737, 1911
EarI	CTCTTC	3 cut(s)	1729, 1737, 1911
EciI	GGCGGA	2 cut(s)	381, 1300
EclXI	CGGCCG	1 cut(s)	351
Eco130I	CCWWGG	3 cut(s)	658, 1803, 2178
Eco31I	GGTCTC	1 cut(s)	449
Eco32I	GATATC	1 cut(s)	2203
Eco47I	GGWCC	1 cut(s)	749
Eco52I	CGGCCG	1 cut(s)	351
Eco57I	CTGAAG	8 cut(s)	206, 534, 774, 855, 1533, 1707, 1761, 1854
Eco88I	CYCGRG	2 cut(s)	2106, 2133
EcoNI	CCTNNNNNAGG	1 cut(s)	1206
EcoO109I	RGGNCCY	2 cut(s)	749, 758
EcoRI	GAATTC	2 cut(s)	1251, 1705
EcoRII	CCWGG	6 cut(s)	494, 711, 1246, 1315, 1483, 1671
EcoRV	GATATC	1 cut(s)	2203
EcoT14I	CCWWGG	3 cut(s)	658, 1803, 2178
EcoT22I	ATGCAT	2 cut(s)	612, 1938
EgeI	GGCGCC	1 cut(s)	76
EheI	GGCGCC	1 cut(s)	76
ErhI	CCWWGG	3 cut(s)	658, 1803, 2178
FalI	AAGNNNNNCTT	6 cut(s)	1827, 1859, 2020, 2052, 2340, 2372
FaqI	GGGAC	1 cut(s)	1033
FauI	CCCGC	2 cut(s)	354, 1860
FauNDI	CATATG	2 cut(s)	1516, 2002
FblI	GTMKAC	1 cut(s)	1799
FspBI	CTAG	4 cut(s)	680, 728, 762, 2061
GlaI	GCGC	3 cut(s)	76, 123, 1438
GsaI	CCCAGC	1 cut(s)	1227
GsuI	CTGGAG	3 cut(s)	705, 1868, 2006
HaeII	RGCGCY	2 cut(s)	78, 1440
HapII	CCGG	9 cut(s)	86, 130, 216, 223, 350, 354, 395, 441, 2156
HgaI	GACGC	2 cut(s)	314, 1030
HhaI	GCGC	3 cut(s)	77, 124, 1439
Hin1I	GRCGYC	2 cut(s)	75, 1571
Hin6I	GCGC	3 cut(s)	75, 122, 1437
HinP1I	GCGC	3 cut(s)	75, 122, 1437
HincII	GTYRAC	1 cut(s)	1332
HindII	GTYRAC	1 cut(s)	1332
HindIII	AAGCTT	1 cut(s)	1411
HpaI	GTTAAC	1 cut(s)	1332
HpaII	CCGG	9 cut(s)	86, 130, 216, 223, 350, 354, 395, 441, 2156
HphI	GGTGA	1 cut(s)	2018
Hpy166II	GTNNAC	8 cut(s)	617, 996, 1048, 1146, 1332, 1397, 1669, 1800
Hpy188I	TCNGA	8 cut(s)	150, 285, 1696, 1819, 1825, 1902, 1950, 2041
Hpy8I	GTNNAC	8 cut(s)	617, 996, 1048, 1146, 1332, 1397, 1669, 1800
Hpy99I	CGWCG	1 cut(s)	104
HpyAV	CCTTC	5 cut(s)	509, 749, 1193, 1592, 1885
HpyCH4III	ACNGT	7 cut(s)	230, 645, 919, 1000, 1039, 1797, 2019
HpyCH4IV	ACGT	2 cut(s)	1571, 2045
HpyF3I	CTNAG	4 cut(s)	38, 923, 1503, 2303
HpySE526I	ACGT	2 cut(s)	1571, 2045
Hsp92I	GRCGYC	2 cut(s)	75, 1571
HspAI	GCGC	3 cut(s)	75, 122, 1437
KasI	GGCGCC	1 cut(s)	74
Kpn2I	TCCGGA	1 cut(s)	2155
KroI	GCCGGC	1 cut(s)	349
KroNI	GCCGGC	1 cut(s)	351
KspAI	GTTAAC	1 cut(s)	1332
Kzo9I	GATC	6 cut(s)	41, 175, 305, 316, 631, 2052
LmnI	GCTCC	4 cut(s)	394, 534, 1220, 2182
LweI	GCATC	5 cut(s)	97, 568, 597, 1700, 1954
MaeI	CTAG	4 cut(s)	680, 728, 762, 2061
MaeII	ACGT	2 cut(s)	1571, 2045
MaeIII	GTNAC	2 cut(s)	580, 1718
MalI	GATC	6 cut(s)	43, 177, 307, 318, 633, 2054
MbiI	CCGCTC	1 cut(s)	301
MboI	GATC	6 cut(s)	41, 175, 305, 316, 631, 2052
MflI	RGATCY	2 cut(s)	41, 175
MhlI	GDGCHC	1 cut(s)	391
MlsI	TGGCCA	2 cut(s)	2177, 2376
MluNI	TGGCCA	2 cut(s)	2177, 2376
Mly113I	GGCGCC	1 cut(s)	75
MlyI	GAGTC	3 cut(s)	22, 168, 696
MmeI	TCCRAC	2 cut(s)	533, 1618
Mox20I	TGGCCA	2 cut(s)	2177, 2376
Mph1103I	ATGCAT	2 cut(s)	612, 1938
MroI	TCCGGA	1 cut(s)	2155
MroNI	GCCGGC	1 cut(s)	349
MroXI	GAANNNNTTC	4 cut(s)	230, 868, 1842, 1980
MscI	TGGCCA	2 cut(s)	2177, 2376
MslI	CAYNNNNRTG	1 cut(s)	1776
Msp20I	TGGCCA	2 cut(s)	2177, 2376
MspA1I	CMGCKG	5 cut(s)	322, 1064, 1457, 1736, 2096
MspI	CCGG	9 cut(s)	86, 130, 216, 223, 350, 354, 395, 441, 2156
MspR9I	CCNGG	6 cut(s)	496, 713, 1248, 1317, 1485, 1673
Mva1269I	GAATGC	1 cut(s)	423
MvaI	CCWGG	6 cut(s)	496, 713, 1248, 1317, 1485, 1673
MvnI	CGCG	2 cut(s)	458, 1867
NaeI	GCCGGC	1 cut(s)	351
NarI	GGCGCC	1 cut(s)	75
NcoI	CCATGG	1 cut(s)	1803
NdeI	CATATG	2 cut(s)	1516, 2002
NdeII	GATC	6 cut(s)	41, 175, 305, 316, 631, 2052
NgoMIV	GCCGGC	1 cut(s)	349
NlaIV	GGNNCC	4 cut(s)	76, 530, 1563, 1656
NmeAIII	GCCGAG	1 cut(s)	408
NmuCI	GTSAC	1 cut(s)	580
NsiI	ATGCAT	2 cut(s)	612, 1938
NspI	RCATGY	3 cut(s)	479, 1099, 1200
PaeI	GCATGC	2 cut(s)	1099, 1200
PaeR7I	CTCGAG	1 cut(s)	2106
PagI	TCATGA	1 cut(s)	340
PaqCI	CACCTGC	1 cut(s)	839
PciI	ACATGT	1 cut(s)	475
PcsI	WCGNNNNNNNCGW	1 cut(s)	366
PctI	GAATGC	1 cut(s)	423
PdiI	GCCGGC	1 cut(s)	351
PdmI	GAANNNNTTC	4 cut(s)	230, 868, 1842, 1980
PfeI	GAWTC	7 cut(s)	357, 1320, 1820, 1852, 2057, 2285, 2353
PfoI	TCCNGGA	2 cut(s)	1246, 1483
PinAI	ACCGGT	1 cut(s)	440
Ple19I	CGATCG	1 cut(s)	308
PleI	GAGTC	3 cut(s)	22, 167, 695
PluTI	GGCGCC	1 cut(s)	78
PpsI	GAGTC	3 cut(s)	22, 167, 695
PpuMI	RGGWCCY	1 cut(s)	749
PscI	ACATGT	1 cut(s)	475
PshBI	ATTAAT	3 cut(s)	803, 1470, 2171
Psp5II	RGGWCCY	1 cut(s)	749
Psp6I	CCWGG	6 cut(s)	494, 711, 1246, 1315, 1483, 1671
PspFI	CCCAGC	1 cut(s)	1223
PspGI	CCWGG	6 cut(s)	494, 711, 1246, 1315, 1483, 1671
PspN4I	GGNNCC	4 cut(s)	76, 530, 1563, 1656
PspPI	GGNCC	4 cut(s)	492, 749, 758, 1869
PspPPI	RGGWCCY	1 cut(s)	749
PsrI	GAACNNNNNNTAC	2 cut(s)	724, 756
PstI	CTGCAG	3 cut(s)	540, 1192, 1462
PstNI	CAGNNNCTG	6 cut(s)	544, 854, 893, 1043, 1067, 1739
PsuI	RGATCY	2 cut(s)	41, 175
PvuI	CGATCG	1 cut(s)	308
PvuII	CAGCTG	4 cut(s)	322, 1064, 1457, 1736
RgaI	GCGATCGC	1 cut(s)	308
RsaI	GTAC	7 cut(s)	17, 474, 505, 576, 733, 1259, 2104
RsaNI	GTAC	7 cut(s)	16, 473, 504, 575, 732, 1258, 2103
RseI	CAYNNNNRTG	1 cut(s)	1776
Sau3AI	GATC	6 cut(s)	41, 175, 305, 316, 631, 2052
Sau96I	GGNCC	4 cut(s)	492, 749, 758, 1869
SchI	GAGTC	3 cut(s)	22, 168, 696
ScrFI	CCNGG	6 cut(s)	496, 713, 1248, 1317, 1485, 1673
SduI	GDGCHC	1 cut(s)	391
SfaAI	GCGATCGC	1 cut(s)	308
SfaNI	GCATC	5 cut(s)	97, 568, 597, 1700, 1954
SfcI	CTRYAG	4 cut(s)	312, 536, 1188, 1458
SfoI	GGCGCC	1 cut(s)	76
Sfr274I	CTCGAG	1 cut(s)	2106
SgfI	GCGATCGC	1 cut(s)	308
SinI	GGWCC	1 cut(s)	749
SlaI	CTCGAG	1 cut(s)	2106
SmiMI	CAYNNNNRTG	1 cut(s)	1776
SmlI	CTYRAG	2 cut(s)	1238, 2106
SmoI	CTYRAG	2 cut(s)	1238, 2106
SphI	GCATGC	2 cut(s)	1099, 1200
SspDI	GGCGCC	1 cut(s)	74
SspI	AATATT	3 cut(s)	627, 1614, 1995
SspMI	CTAG	4 cut(s)	680, 728, 762, 2061
StyD4I	CCNGG	6 cut(s)	494, 711, 1246, 1315, 1483, 1671
StyI	CCWWGG	3 cut(s)	658, 1803, 2178
TaaI	ACNGT	7 cut(s)	230, 645, 919, 1000, 1039, 1797, 2019
TaiI	ACGT	2 cut(s)	1574, 2048
TaqI	TCGA	7 cut(s)	47, 243, 360, 562, 934, 2055, 2107
TatI	WGTACW	4 cut(s)	15, 472, 1257, 2102
TauI	GCSGC	3 cut(s)	304, 458, 887
TfiI	GAWTC	7 cut(s)	357, 1320, 1820, 1852, 2057, 2285, 2353
TscAI	CASTG	2 cut(s)	1044, 1527
TseFI	GTSAC	1 cut(s)	580
Tsp45I	GTSAC	1 cut(s)	580
TspDTI	ATGAA	6 cut(s)	387, 1767, 1989, 2111, 2267, 2324
TspGWI	ACGGA	2 cut(s)	521, 1717
TspRI	CASTG	2 cut(s)	1044, 1527
VpaK11BI	GGWCC	1 cut(s)	749
VspI	ATTAAT	3 cut(s)	803, 1470, 2171
XagI	CCTNNNNNAGG	1 cut(s)	1206
XapI	RAATTY	3 cut(s)	864, 1251, 1705
XceI	RCATGY	3 cut(s)	479, 1099, 1200
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2185
XhoI	CTCGAG	1 cut(s)	2106
XmiI	GTMKAC	1 cut(s)	1799
XmnI	GAANNNNTTC	4 cut(s)	230, 868, 1842, 1980
XspI	CTAG	4 cut(s)	680, 728, 762, 2061
ZraI	GACGTC	1 cut(s)	1572
Zsp2I	ATGCAT	2 cut(s)	612, 1938

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.