MD03G1051700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015586
Physical Location & Seq
Forward (+)
4133040 .. 4135780
2741 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1051700.v1.1.491

Sequence Viewer

Length: 1116 bp
ATGCTCGATGTCCACGACCGGCCCTTCAACTTGTCCTTTTATTACCGTCAGATGGGGGACAAGGCAACATTGAGCAAGGCAATAATGGAGCTTGAAGACATGTACCAAGGCATCCCAGATGAGTCAGTTAACCTAACTTTCCAGGACTTTGCAGAGCTCAAAATACACCAAGTTACATCAGAAAAGAAGAAAAAACCTACTCCATTAATACTGGATCCAATCTCTGAACTCAAATCTACCCCACAAGCATCATCTCCCTCTCCTTTCACCAAACTCCCCAGCCTTGACTTCAACCGTGCCTTGCAAGCATCCAGAGACAACCACCGCCACCACCACAACCGCCACCACATCAACGAAAACAATGTCCCCGTCGGTCCATATGGCATAGGTGATAACAATGATCATAATTATCATCAGCATGGGCATGCAGCACGTGGAGGTCACGGTCGATATATGGGCGGTGGTGACCATCATAGCCATCAGATGCATCATGGTCATGATGGCCATGCAGCTAATAATGATCATCATATGAATATGAGAAGTCCTGGTCCCACTTGTAATTCCGGTATGAAGCACGGATTGGAGACTAGCTTGGGGGCATTTGATCATGACGTGAGTGGTGTGAGCGGTGTAAGCATGGCTTCCATGTACCAGAAAGAGAGAGGAGGAATACGAAGGCCTGGGATTCCCCACTCTAATATCTGCACCATTTGCAGTACCTACATCTATATTTTCCGCCACCGTTGCCTGGTATGTGGAAGAGTGTATTGTAGGCGATGTGTGATGGTAGGGATGGCAGAAATGACAGAAGGTAGAAAATGCGTTGAGTGTTTAGGGAGGAGATTCAGTCAAAGGTACATACAAAGAGCAGGGAAGGTTGGGTGTTGCTCAAGGTACCCTTCCAGTGTGAAGCAAGCAGAGCTCAAGTGGGCTGAGAAAGGGCCGAGAAGAAGCGCCGGCAATGGGGGCTACGGCCGGAGCTCGATGACGTTGCCGAGATCAAAAAGCCCGATGACGCCGATGACTCCGAGGACCCCAAACAGAGGCCATGCTAGTGGCCCAAATTCTTTTGTCGTGAATTATTCACCAAGTCATCGCCACCACCTTCCTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

372

Amino Acids

41.58

Weight (kDa)

9.51

Isoelectric Point (pI)

49.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015586)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g40511
malus_domestica MD03G1051700.v1.1 MD11G1053800.v1.1
prunus_persica Prupe.6G041100_v2.0.a1
pyrus_communis pycom11g04340
rosa_chinensis RchiOBHm_Chr5g0073191
rosa_laevigata RLG00000036372
rosa_multiflora Rmu_sc0001887.1_g000011
rosa_roxburghii Rroxscaffold_1G00008000
rosa_rugosa Rorug05G0421900
rosa_samantha Rh5AG478800 Rh5BG499500 Rh5CG522700 Rh5DG511600
rosa_wichuraiana Rw5G044500 Rw5G044510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 894
AccB1I GGYRCC 1 cut(s) 894
AccBSI CCGCTC 1 cut(s) 627
AciI CCGC 5 cut(s) 325, 340, 459, 627, 736
AclWI GGATC 2 cut(s) 209, 222
AcoI YGGCCR 2 cut(s) 502, 973
AcsI RAATTY 1 cut(s) 1063
AcvI CACGTG 1 cut(s) 434
AcyI GRCGYC 1 cut(s) 1016
AfaI GTAC 5 cut(s) 104, 650, 718, 857, 896
AfiI CCNNNNNNNGG 4 cut(s) 52, 748, 963, 1043
AflIII ACRYGT 1 cut(s) 99
AgsI TTSAA 3 cut(s) 28, 95, 292
AjiI CACGTC 1 cut(s) 613
AjnI CCWGG 4 cut(s) 141, 544, 679, 747
AjuI GAANNNNNNNTTGG 2 cut(s) 563, 595
AluBI AGCT 6 cut(s) 91, 157, 512, 591, 922, 981
AluI AGCT 6 cut(s) 91, 157, 512, 591, 922, 981
Alw21I GWGCWC 3 cut(s) 159, 924, 983
Alw26I GTCTC 2 cut(s) 309, 578
AlwI GGATC 2 cut(s) 209, 222
AoxI GGCC 7 cut(s) 20, 502, 677, 941, 973, 1045, 1057
ApeKI GCWGC 2 cut(s) 428, 509
ApoI RAATTY 1 cut(s) 1063
ArsI GACNNNNNNTTYG 2 cut(s) 348, 380
AseI ATTAAT 1 cut(s) 206
Asp718I GGTACC 1 cut(s) 894
AspLEI GCGC 1 cut(s) 956
AspS9I GGNCC 6 cut(s) 21, 374, 548, 941, 1032, 1058
AsuHPI GGTGA 4 cut(s) 259, 401, 476, 1077
AvaII GGWCC 3 cut(s) 374, 548, 1032
BalI TGGCCA 1 cut(s) 504
BamHI GGATCC 1 cut(s) 214
BanI GGYRCC 1 cut(s) 894
BanII GRGCYC 3 cut(s) 159, 924, 983
BbrPI CACGTG 1 cut(s) 434
BbsI GAAGAC 1 cut(s) 102
Bbv12I GWGCWC 3 cut(s) 159, 924, 983
BbvI GCAGC 2 cut(s) 440, 521
BccI CCATC 6 cut(s) 46, 477, 486, 494, 778, 787
BceAI ACGGC 1 cut(s) 988
BcgI CGANNNNNNTGC 2 cut(s) 973, 1007
BciT130I CCWGG 4 cut(s) 143, 546, 681, 749
BclI TGATCA 3 cut(s) 400, 520, 604
BcoDI GTCTC 2 cut(s) 309, 578
BfaI CTAG 3 cut(s) 588, 1053, 1114
BfoI RGCGCY 1 cut(s) 957
BisI GCNGC 2 cut(s) 429, 510
BlsI GCNGC 2 cut(s) 430, 511
Bme1390I CCNGG 4 cut(s) 143, 546, 681, 749
Bme18I GGWCC 3 cut(s) 374, 548, 1032
BmgBI CACGTC 1 cut(s) 613
BmgT120I GGNCC 6 cut(s) 21, 374, 548, 941, 1032, 1058
BmiI GGNNCC 4 cut(s) 216, 550, 896, 1034
BmrFI CCNGG 4 cut(s) 143, 546, 681, 749
BmsI GCATC 5 cut(s) 120, 257, 317, 474, 496
BpiI GAAGAC 1 cut(s) 102
BpuEI CTTGAG 2 cut(s) 874, 908
BsaAI YACGTR 1 cut(s) 434
BsaHI GRCGYC 1 cut(s) 1016
BsaJI CCNNGG 3 cut(s) 106, 680, 1028
BsaWI WCCGGW 1 cut(s) 563
BsaXI ACNNNNNCTCC 2 cut(s) 832, 862
Bsc4I CCNNNNNNNGG 4 cut(s) 52, 748, 963, 1043
Bse118I RCCGGY 2 cut(s) 18, 956
Bse1I ACTGG 2 cut(s) 216, 903
Bse3DI GCAATG 1 cut(s) 967
BseBI CCWGG 4 cut(s) 143, 546, 681, 749
BseDI CCNNGG 3 cut(s) 106, 680, 1028
BseGI GGATG 3 cut(s) 111, 308, 798
BseLI CCNNNNNNNGG 4 cut(s) 52, 748, 963, 1043
BseMI GCAATG 1 cut(s) 967
BseMII CTCAG 1 cut(s) 924
BseNI ACTGG 2 cut(s) 216, 903
BseRI GAGGAG 2 cut(s) 678, 853
BseX3I CGGCCG 1 cut(s) 973
BseXI GCAGC 2 cut(s) 440, 521
BseYI CCCAGC 1 cut(s) 278
BsgI GTGCAG 1 cut(s) 688
Bsh1285I CGRYCG 3 cut(s) 19, 448, 976
BshFI GGCC 7 cut(s) 22, 504, 679, 943, 975, 1047, 1059
BshNI GGYRCC 1 cut(s) 894
BsiEI CGRYCG 3 cut(s) 19, 448, 976
BsiHKAI GWGCWC 3 cut(s) 159, 924, 983
BsiSI CCGG 4 cut(s) 19, 564, 957, 976
BslFI GGGAC 3 cut(s) 71, 350, 534
BslI CCNNNNNNNGG 4 cut(s) 52, 748, 963, 1043
BsmAI GTCTC 2 cut(s) 309, 578
BsmFI GGGAC 3 cut(s) 71, 350, 534
BsnI GGCC 7 cut(s) 22, 504, 679, 943, 975, 1047, 1059
Bsp1286I GDGCHC 3 cut(s) 159, 924, 983
Bsp143I GATC 5 cut(s) 214, 400, 520, 604, 998
BspACI CCGC 5 cut(s) 325, 340, 459, 627, 736
BspANI GGCC 7 cut(s) 22, 504, 679, 943, 975, 1047, 1059
BspCNI CTCAG 1 cut(s) 925
BspHI TCATGA 2 cut(s) 496, 607
BspLI GGNNCC 4 cut(s) 216, 550, 896, 1034
BspPI GGATC 2 cut(s) 209, 222
BspT107I GGYRCC 1 cut(s) 894
BsrBI CCGCTC 1 cut(s) 627
BsrDI GCAATG 1 cut(s) 967
BsrFI RCCGGY 2 cut(s) 18, 956
BsrI ACTGG 2 cut(s) 216, 903
BssAI RCCGGY 2 cut(s) 18, 956
BssECI CCNNGG 3 cut(s) 106, 680, 1028
BssMI GATC 5 cut(s) 214, 400, 520, 604, 998
BssNI GRCGYC 1 cut(s) 1016
BssT1I CCWWGG 1 cut(s) 106
Bst2UI CCWGG 4 cut(s) 143, 546, 681, 749
Bst4CI ACNGT 4 cut(s) 47, 296, 446, 743
Bst6I CTCTTC 1 cut(s) 754
BstACI GRCGYC 1 cut(s) 1016
BstAPI GCANNNNNTGC 1 cut(s) 711
BstBAI YACGTR 1 cut(s) 434
BstC8I GCNNGC 4 cut(s) 306, 426, 915, 958
BstDEI CTNAG 1 cut(s) 933
BstEII GGTNACC 1 cut(s) 464
BstF5I GGATG 3 cut(s) 111, 308, 798
BstH2I RGCGCY 1 cut(s) 957
BstHHI GCGC 1 cut(s) 956
BstKTI GATC 5 cut(s) 217, 403, 523, 607, 1001
BstMAI GTCTC 2 cut(s) 309, 578
BstMBI GATC 5 cut(s) 214, 400, 520, 604, 998
BstMCI CGRYCG 3 cut(s) 19, 448, 976
BstMWI GCNNNNNNNGC 6 cut(s) 305, 633, 711, 744, 919, 966
BstNI CCWGG 4 cut(s) 143, 546, 681, 749
BstNSI RCATGY 2 cut(s) 103, 428
BstPI GGTNACC 1 cut(s) 464
BstSCI CCNGG 4 cut(s) 141, 544, 679, 747
BstV1I GCAGC 2 cut(s) 440, 521
BstV2I GAAGAC 1 cut(s) 102
BstX2I RGATCY 1 cut(s) 214
BstXI CCANNNNNNTGG 1 cut(s) 1055
BstYI RGATCY 1 cut(s) 214
BstZI CGGCCG 1 cut(s) 973
BsuRI GGCC 7 cut(s) 22, 504, 679, 943, 975, 1047, 1059
BtgZI GCGATG 2 cut(s) 790, 1079
BtrI CACGTC 1 cut(s) 613
BtsCI GGATG 3 cut(s) 111, 308, 798
BtsIMutI CAGTG 1 cut(s) 910
Cac8I GCNNGC 4 cut(s) 306, 426, 915, 958
CciI TCATGA 2 cut(s) 496, 607
CfoI GCGC 1 cut(s) 956
Cfr10I RCCGGY 2 cut(s) 18, 956
Cfr13I GGNCC 6 cut(s) 21, 374, 548, 941, 1032, 1058
CseI GACGC 1 cut(s) 1024
Csp6I GTAC 5 cut(s) 103, 649, 717, 856, 895
CviQI GTAC 5 cut(s) 103, 649, 717, 856, 895
DdeI CTNAG 1 cut(s) 933
DpnI GATC 5 cut(s) 216, 402, 522, 606, 1000
DpnII GATC 5 cut(s) 214, 400, 520, 604, 998
EaeI YGGCCR 2 cut(s) 502, 973
EagI CGGCCG 1 cut(s) 973
Eam1104I CTCTTC 1 cut(s) 754
EarI CTCTTC 1 cut(s) 754
EciI GGCGGA 1 cut(s) 725
Ecl136II GAGCTC 3 cut(s) 157, 922, 981
EclXI CGGCCG 1 cut(s) 973
Eco130I CCWWGG 1 cut(s) 106
Eco147I AGGCCT 1 cut(s) 679
Eco24I GRGCYC 3 cut(s) 159, 924, 983
Eco47I GGWCC 3 cut(s) 374, 548, 1032
Eco52I CGGCCG 1 cut(s) 973
Eco53kI GAGCTC 3 cut(s) 157, 922, 981
Eco72I CACGTG 1 cut(s) 434
Eco91I GGTNACC 1 cut(s) 464
EcoICRI GAGCTC 3 cut(s) 157, 922, 981
EcoO109I RGGNCCY 1 cut(s) 1032
EcoO65I GGTNACC 1 cut(s) 464
EcoRII CCWGG 4 cut(s) 141, 544, 679, 747
EcoT14I CCWWGG 1 cut(s) 106
EcoT22I ATGCAT 1 cut(s) 489
EcoT38I GRGCYC 3 cut(s) 159, 924, 983
ErhI CCWWGG 1 cut(s) 106
FalI AAGNNNNNCTT 4 cut(s) 625, 657, 883, 915
FaqI GGGAC 3 cut(s) 71, 350, 534
FauNDI CATATG 2 cut(s) 379, 528
FbaI TGATCA 3 cut(s) 400, 520, 604
Fnu4HI GCNGC 2 cut(s) 429, 510
FokI GGATG 3 cut(s) 98, 295, 805
FriOI GRGCYC 3 cut(s) 159, 924, 983
Fsp4HI GCNGC 2 cut(s) 429, 510
FspBI CTAG 3 cut(s) 588, 1053, 1114
GlaI GCGC 1 cut(s) 955
GluI GCNGC 2 cut(s) 429, 510
GsaI CCCAGC 1 cut(s) 282
HaeII RGCGCY 1 cut(s) 957
HaeIII GGCC 7 cut(s) 22, 504, 679, 943, 975, 1047, 1059
HapII CCGG 4 cut(s) 19, 564, 957, 976
HgaI GACGC 1 cut(s) 1024
HhaI GCGC 1 cut(s) 956
Hin1I GRCGYC 1 cut(s) 1016
Hin6I GCGC 1 cut(s) 954
HinP1I GCGC 1 cut(s) 954
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HinfI GANTC 4 cut(s) 122, 685, 843, 1024
HpaI GTTAAC 1 cut(s) 130
HpaII CCGG 4 cut(s) 19, 564, 957, 976
HphI GGTGA 4 cut(s) 259, 401, 476, 1077
Hpy166II GTNNAC 2 cut(s) 13, 130
Hpy188I TCNGA 5 cut(s) 51, 181, 226, 483, 1029
Hpy188III TCNNGA 4 cut(s) 312, 497, 608, 1075
Hpy8I GTNNAC 2 cut(s) 13, 130
Hpy99I CGWCG 1 cut(s) 374
HpyAV CCTTC 6 cut(s) 34, 669, 803, 868, 909, 1115
HpyCH4III ACNGT 4 cut(s) 47, 296, 446, 743
HpyCH4IV ACGT 3 cut(s) 433, 612, 989
HpyCH4V TGCA 7 cut(s) 152, 304, 428, 487, 509, 705, 714
HpyF10VI GCNNNNNNNGC 6 cut(s) 305, 633, 711, 744, 919, 966
HpyF3I CTNAG 1 cut(s) 933
HpySE526I ACGT 3 cut(s) 433, 612, 989
Hsp92I GRCGYC 1 cut(s) 1016
HspAI GCGC 1 cut(s) 954
KpnI GGTACC 1 cut(s) 898
KroI GCCGGC 1 cut(s) 956
KroNI GCCGGC 1 cut(s) 958
Ksp22I TGATCA 3 cut(s) 400, 520, 604
KspAI GTTAAC 1 cut(s) 130
Kzo9I GATC 5 cut(s) 214, 400, 520, 604, 998
LmnI GCTCC 2 cut(s) 88, 978
Lsp1109I GCAGC 2 cut(s) 440, 521
LweI GCATC 5 cut(s) 120, 257, 317, 474, 496
MaeI CTAG 3 cut(s) 588, 1053, 1114
MaeII ACGT 3 cut(s) 433, 612, 989
MaeIII GTNAC 3 cut(s) 172, 440, 464
MalI GATC 5 cut(s) 216, 402, 522, 606, 1000
MbiI CCGCTC 1 cut(s) 627
MboI GATC 5 cut(s) 214, 400, 520, 604, 998
MboII GAAGA 4 cut(s) 107, 199, 771, 960
MflI RGATCY 1 cut(s) 214
MhlI GDGCHC 3 cut(s) 159, 924, 983
MlsI TGGCCA 1 cut(s) 504
MluCI AATT 4 cut(s) 406, 559, 1063, 1078
MluNI TGGCCA 1 cut(s) 504
MlyI GAGTC 2 cut(s) 131, 1018
MnlI CCTC 7 cut(s) 268, 431, 656, 659, 831, 1023, 1037
Mox20I TGGCCA 1 cut(s) 504
Mph1103I ATGCAT 1 cut(s) 489
MroNI GCCGGC 1 cut(s) 956
MscI TGGCCA 1 cut(s) 504
MseI TTAA 2 cut(s) 129, 206
MslI CAYNNNNRTG 4 cut(s) 417, 423, 495, 1053
Msp20I TGGCCA 1 cut(s) 504
MspI CCGG 4 cut(s) 19, 564, 957, 976
MspR9I CCNGG 4 cut(s) 143, 546, 681, 749
MvaI CCWGG 4 cut(s) 143, 546, 681, 749
MwoI GCNNNNNNNGC 6 cut(s) 305, 633, 711, 744, 919, 966
NaeI GCCGGC 1 cut(s) 958
NdeI CATATG 2 cut(s) 379, 528
NdeII GATC 5 cut(s) 214, 400, 520, 604, 998
NgoMIV GCCGGC 1 cut(s) 956
NlaIV GGNNCC 4 cut(s) 216, 550, 896, 1034
NmeAIII GCCGAG 2 cut(s) 969, 1020
NmuCI GTSAC 2 cut(s) 440, 464
NsiI ATGCAT 1 cut(s) 489
NspI RCATGY 2 cut(s) 103, 428
PaeI GCATGC 1 cut(s) 428
PagI TCATGA 2 cut(s) 496, 607
PceI AGGCCT 1 cut(s) 679
PciI ACATGT 1 cut(s) 99
PcsI WCGNNNNNNNCGW 1 cut(s) 12
PdiI GCCGGC 1 cut(s) 958
PfeI GAWTC 2 cut(s) 685, 843
PfoI TCCNGGA 1 cut(s) 141
PkrI GCNGC 2 cut(s) 430, 511
PleI GAGTC 2 cut(s) 130, 1018
PmaCI CACGTG 1 cut(s) 434
PmlI CACGTG 1 cut(s) 434
PpsI GAGTC 2 cut(s) 130, 1018
Ppu21I YACGTR 1 cut(s) 434
PpuMI RGGWCCY 1 cut(s) 1032
PscI ACATGT 1 cut(s) 99
PshBI ATTAAT 1 cut(s) 206
Psp124BI GAGCTC 3 cut(s) 159, 924, 983
Psp5II RGGWCCY 1 cut(s) 1032
Psp6I CCWGG 4 cut(s) 141, 544, 679, 747
PspCI CACGTG 1 cut(s) 434
PspEI GGTNACC 1 cut(s) 464
PspFI CCCAGC 1 cut(s) 278
PspGI CCWGG 4 cut(s) 141, 544, 679, 747
PspN4I GGNNCC 4 cut(s) 216, 550, 896, 1034
PspPI GGNCC 6 cut(s) 21, 374, 548, 941, 1032, 1058
PspPPI RGGWCCY 1 cut(s) 1032
PsuI RGATCY 1 cut(s) 214
RsaI GTAC 5 cut(s) 104, 650, 718, 857, 896
RsaNI GTAC 5 cut(s) 103, 649, 717, 856, 895
RseI CAYNNNNRTG 4 cut(s) 417, 423, 495, 1053
SacI GAGCTC 3 cut(s) 159, 924, 983
SaqAI TTAA 2 cut(s) 129, 206
SatI GCNGC 2 cut(s) 429, 510
Sau3AI GATC 5 cut(s) 214, 400, 520, 604, 998
Sau96I GGNCC 6 cut(s) 21, 374, 548, 941, 1032, 1058
SchI GAGTC 2 cut(s) 131, 1018
ScrFI CCNGG 4 cut(s) 143, 546, 681, 749
SduI GDGCHC 3 cut(s) 159, 924, 983
SfaNI GCATC 5 cut(s) 120, 257, 317, 474, 496
SinI GGWCC 3 cut(s) 374, 548, 1032
SmiMI CAYNNNNRTG 4 cut(s) 417, 423, 495, 1053
SmlI CTYRAG 2 cut(s) 889, 923
SmoI CTYRAG 2 cut(s) 889, 923
SphI GCATGC 1 cut(s) 428
Sse9I AATT 4 cut(s) 406, 559, 1063, 1078
SseBI AGGCCT 1 cut(s) 679
SsiI CCGC 5 cut(s) 325, 340, 459, 627, 736
SspMI CTAG 3 cut(s) 588, 1053, 1114
SstI GAGCTC 3 cut(s) 159, 924, 983
StuI AGGCCT 1 cut(s) 679
StyD4I CCNGG 4 cut(s) 141, 544, 679, 747
StyI CCWWGG 1 cut(s) 106
TaaI ACNGT 4 cut(s) 47, 296, 446, 743
TaiI ACGT 3 cut(s) 436, 615, 992
TaqI TCGA 3 cut(s) 6, 448, 983
TaqII GACCGA 1 cut(s) 362
TasI AATT 4 cut(s) 406, 559, 1063, 1078
TfiI GAWTC 2 cut(s) 685, 843
Tru1I TTAA 2 cut(s) 129, 206
Tru9I TTAA 2 cut(s) 129, 206
TscAI CASTG 1 cut(s) 910
TseFI GTSAC 2 cut(s) 440, 464
TseI GCWGC 2 cut(s) 428, 509
Tsp45I GTSAC 2 cut(s) 440, 464
TspDTI ATGAA 2 cut(s) 545, 584
TspGWI ACGGA 1 cut(s) 591
TspRI CASTG 1 cut(s) 910
VpaK11BI GGWCC 3 cut(s) 374, 548, 1032
VspI ATTAAT 1 cut(s) 206
XapI RAATTY 1 cut(s) 1063
XceI RCATGY 2 cut(s) 103, 428
XspI CTAG 3 cut(s) 588, 1053, 1114
Zsp2I ATGCAT 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.