MD03G1068700.v1.1

Belongs to the bacterial ribosomal protein bS18 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0013090
Physical Location & Seq
Forward (+)
5590515 .. 5592063
1549 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1068700.v1.1.491

Sequence Viewer

Length: 339 bp
ATGGAAGTACTATGTGACTCTATGTATGAGTTTGAAGTAACTACAAAGGAAGTTCTTAGAAAAGCTGATTTCAGGAATGTGAGATTCCTTGCGAATTTCATAACAGAGGCTGGAATTCTCATCAAAAGAAGCAAGACTGGAATTAGCGCCAAAGCTCAGAGGAAGGTCGCCAGGGAAATCAAAACAGCTCGAGCTTTTGGTTTAATGCCTTTCACGACAATGGGGACAAAATCATTTGTTTTTGGGAAAACTATGGAGAATCTAGATGAAGATTATGAGTACGAAAGTTATGATAACCATATGGGTGATGCTGATGGAGGAGATCCACTGGCGCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_S18

Protein Analysis

113

Amino Acids

12.68

Weight (kDa)

5.44

Isoelectric Point (pI)

31.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S18 PF01084 21 - 69 2.8e-16 Ribosomal protein S18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013090)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07210
fragaria_vesca FvH4_3g38720
malus_domestica MD03G1068600.v1.1 MD03G1068700.v1.1 MD11G1073200.v1.1
prunus_persica Prupe.6G056500_v2.0.a1 Prupe.6G056500_v2.0.a1
pyrus_communis pycom11g06070
rosa_chinensis RchiOBHm_Chr5g0069871
rosa_laevigata RLG00000036104
rosa_multiflora Rmu_sc0000738.1_g000001
rosa_roxburghii Rroxscaffold_1G00011180
rosa_rugosa Rorug05G0400400
rosa_samantha Rh5AG457500 Rh5BG476300 Rh5CG500100 Rh5DG488100
rosa_wichuraiana Rw5G042710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 331
AclWI GGATC 1 cut(s) 317
AcsI RAATTY 2 cut(s) 94, 114
AcyI GRCGYC 1 cut(s) 332
AfaI GTAC 2 cut(s) 9, 281
AgsI TTSAA 1 cut(s) 35
AjnI CCWGG 1 cut(s) 170
AluBI AGCT 4 cut(s) 65, 155, 188, 194
AluI AGCT 4 cut(s) 65, 155, 188, 194
AlwI GGATC 1 cut(s) 317
AlwNI CAGNNNCTG 1 cut(s) 110
Ama87I CYCGRG 1 cut(s) 189
ApoI RAATTY 2 cut(s) 94, 114
AspLEI GCGC 2 cut(s) 149, 334
AsuHPI GGTGA 1 cut(s) 317
AvaI CYCGRG 1 cut(s) 189
BanI GGYRCC 1 cut(s) 331
BccI CCATC 1 cut(s) 308
BceAI ACGGC 1 cut(s) 319
BciT130I CCWGG 1 cut(s) 172
BfaI CTAG 1 cut(s) 263
BfoI RGCGCY 2 cut(s) 150, 335
BmcAI AGTACT 1 cut(s) 9
Bme1390I CCNGG 1 cut(s) 172
BmeT110I CYCGRG 1 cut(s) 189
BmiI GGNNCC 1 cut(s) 333
BmrFI CCNGG 1 cut(s) 172
BmsI GCATC 1 cut(s) 298
BsaHI GRCGYC 1 cut(s) 332
BsaJI CCNNGG 1 cut(s) 171
Bse1I ACTGG 2 cut(s) 142, 333
BseBI CCWGG 1 cut(s) 172
BseDI CCNNGG 1 cut(s) 171
BseMII CTCAG 1 cut(s) 170
BseNI ACTGG 2 cut(s) 142, 333
BseRI GAGGAG 1 cut(s) 333
BshNI GGYRCC 1 cut(s) 331
BsiHKCI CYCGRG 1 cut(s) 189
BslFI GGGAC 1 cut(s) 238
BsmFI GGGAC 1 cut(s) 238
BsoBI CYCGRG 1 cut(s) 189
Bsp143I GATC 1 cut(s) 322
BspCNI CTCAG 1 cut(s) 169
BspLI GGNNCC 1 cut(s) 333
BspPI GGATC 1 cut(s) 317
BspT107I GGYRCC 1 cut(s) 331
BsrI ACTGG 2 cut(s) 142, 333
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 1 cut(s) 322
BssNI GRCGYC 1 cut(s) 332
Bst2UI CCWGG 1 cut(s) 172
BstACI GRCGYC 1 cut(s) 332
BstDEI CTNAG 2 cut(s) 56, 156
BstH2I RGCGCY 2 cut(s) 150, 335
BstHHI GCGC 2 cut(s) 149, 334
BstKTI GATC 1 cut(s) 325
BstMBI GATC 1 cut(s) 322
BstNI CCWGG 1 cut(s) 172
BstSCI CCNGG 1 cut(s) 170
BstX2I RGATCY 1 cut(s) 322
BstYI RGATCY 1 cut(s) 322
BtsIMutI CAGTG 1 cut(s) 326
CaiI CAGNNNCTG 1 cut(s) 110
CfoI GCGC 2 cut(s) 149, 334
Csp6I GTAC 2 cut(s) 8, 280
CviJI RGCY 5 cut(s) 65, 110, 155, 188, 194
CviKI_1 RGCY 5 cut(s) 65, 110, 155, 188, 194
CviQI GTAC 2 cut(s) 8, 280
DdeI CTNAG 2 cut(s) 56, 156
DinI GGCGCC 1 cut(s) 333
DpnI GATC 1 cut(s) 324
DpnII GATC 1 cut(s) 322
Eco88I CYCGRG 1 cut(s) 189
EcoRI GAATTC 1 cut(s) 114
EcoRII CCWGG 1 cut(s) 170
EgeI GGCGCC 1 cut(s) 333
EheI GGCGCC 1 cut(s) 333
FaiI YATR 9 cut(s) 13, 23, 27, 101, 254, 276, 291, 300, 302
FaqI GGGAC 1 cut(s) 238
FauNDI CATATG 1 cut(s) 300
FspBI CTAG 1 cut(s) 263
GlaI GCGC 2 cut(s) 148, 333
HaeII RGCGCY 2 cut(s) 150, 335
HhaI GCGC 2 cut(s) 149, 334
Hin1I GRCGYC 1 cut(s) 332
Hin6I GCGC 2 cut(s) 147, 332
HinP1I GCGC 2 cut(s) 147, 332
HinfI GANTC 3 cut(s) 17, 84, 259
HphI GGTGA 1 cut(s) 317
Hpy188I TCNGA 1 cut(s) 159
Hpy188III TCNNGA 3 cut(s) 73, 214, 263
HpyAV CCTTC 1 cut(s) 157
HpyF3I CTNAG 2 cut(s) 56, 156
Hsp92I GRCGYC 1 cut(s) 332
HspAI GCGC 2 cut(s) 147, 332
KasI GGCGCC 1 cut(s) 331
Kzo9I GATC 1 cut(s) 322
LpnPI CCDG 6 cut(s) 58, 96, 123, 157, 184, 314
LweI GCATC 1 cut(s) 298
MaeI CTAG 1 cut(s) 263
MaeIII GTNAC 2 cut(s) 14, 37
MalI GATC 1 cut(s) 324
MboI GATC 1 cut(s) 322
MboII GAAGA 1 cut(s) 281
MflI RGATCY 1 cut(s) 322
MluCI AATT 3 cut(s) 94, 114, 141
Mly113I GGCGCC 1 cut(s) 332
MlyI GAGTC 1 cut(s) 11
MnlI CCTC 3 cut(s) 100, 153, 311
MseI TTAA 1 cut(s) 203
MslI CAYNNNNRTG 2 cut(s) 218, 303
MspR9I CCNGG 1 cut(s) 172
MvaI CCWGG 1 cut(s) 172
NarI GGCGCC 1 cut(s) 332
NdeI CATATG 1 cut(s) 300
NdeII GATC 1 cut(s) 322
NlaIV GGNNCC 1 cut(s) 333
NmuCI GTSAC 1 cut(s) 14
PaeR7I CTCGAG 1 cut(s) 189
PfeI GAWTC 2 cut(s) 84, 259
PleI GAGTC 1 cut(s) 11
PluTI GGCGCC 1 cut(s) 335
PpsI GAGTC 1 cut(s) 11
Psp6I CCWGG 1 cut(s) 170
PspGI CCWGG 1 cut(s) 170
PspN4I GGNNCC 1 cut(s) 333
PspXI VCTCGAGB 1 cut(s) 189
PstNI CAGNNNCTG 1 cut(s) 110
PsuI RGATCY 1 cut(s) 322
RsaI GTAC 2 cut(s) 9, 281
RsaNI GTAC 2 cut(s) 8, 280
RseI CAYNNNNRTG 2 cut(s) 218, 303
SaqAI TTAA 1 cut(s) 203
Sau3AI GATC 1 cut(s) 322
ScaI AGTACT 1 cut(s) 9
SchI GAGTC 1 cut(s) 11
ScrFI CCNGG 1 cut(s) 172
SetI ASST 5 cut(s) 67, 157, 168, 190, 196
SfaNI GCATC 1 cut(s) 298
SfoI GGCGCC 1 cut(s) 333
Sfr274I CTCGAG 1 cut(s) 189
SlaI CTCGAG 1 cut(s) 189
SmiMI CAYNNNNRTG 2 cut(s) 218, 303
SmlI CTYRAG 1 cut(s) 189
SmoI CTYRAG 1 cut(s) 189
Sse9I AATT 3 cut(s) 94, 114, 141
SspDI GGCGCC 1 cut(s) 331
SspMI CTAG 1 cut(s) 263
StyD4I CCNGG 1 cut(s) 170
TaqI TCGA 1 cut(s) 190
TasI AATT 3 cut(s) 94, 114, 141
TatI WGTACW 1 cut(s) 7
TfiI GAWTC 2 cut(s) 84, 259
Tru1I TTAA 1 cut(s) 203
Tru9I TTAA 1 cut(s) 203
TscAI CASTG 1 cut(s) 333
TseFI GTSAC 1 cut(s) 14
Tsp45I GTSAC 1 cut(s) 14
TspDTI ATGAA 2 cut(s) 88, 282
TspRI CASTG 1 cut(s) 333
XapI RAATTY 2 cut(s) 94, 114
XbaI TCTAGA 1 cut(s) 262
XhoI CTCGAG 1 cut(s) 189
XspI CTAG 1 cut(s) 263
ZrmI AGTACT 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.