MD03G1112700.v1.1

E3 SUMO-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0005389
Physical Location & Seq
Forward (+)
9900447 .. 9920782
20336 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1112700.v1.1.491

Sequence Viewer

Length: 2661 bp
ATGGATTTGGTAGCTAGTTGCAAGGAAAAATTGGCATATTTTCGAATAAAAGAGCTCAAAGATGTTCTCACCCAATTAGGTCTTTCGAAGCAGGGGAAGAAGCAGGATCTTGTTGACCGGATATTAGCGCTTCTTTCTGATGAACAAGTTTCTAAAATGTGGGCAAGGAAGAATGCAGTTGGAAAGGAACAGGTGGCGGAACTTGTAGATGACATCTATAGGAAAATGCAGATTTCTGGTGCCCCTGATTTAGCATCAAAGGGACAATGTATCTCTGATAGTAGTAATGTGAAAATAAAAGGCGAATTTGAGGATCCCTACCACTCAGATATAAAGGTTCGCTGTCTCTGCGGGAATTCATTAGAGACAGAGTCAATGATTAAGTGTGAAGATCCAAGATGCCAAGTGTGGCAACACATGGGTTGTGTTATAGTTCCAGAGAAACCTATGGAAGGCAATCCACCAGTTCCTGAGTTATTTTATTGTGAACTTTGTCGACTAAGTCGGGCTGATCCGTTCTGGGTAACTATTCAACATCCTTTACAGCCCGTTAAATTGAATGCTACAAATATTCCAACTGATGGTTCGAATCCAGTGCAAAATGTGGAGAAAACATTTCAACTCACAAGGGCAGACAAGGACTTGCTGTCTAAACAAGAATACGATGTTCAGGTTTGGTGTATGCTTCTAAATGACAAAGTTGCATTCAGGATGCAATGGCCGCAACATGCAGATCTACAGGTCAATGGTGTGCCTGTTCGTGCAATCTATAGACCTGGCTCCCAACTTCTTGGTGCTAATGGTCGTGATGATGGTCCAATTATCACACCATACACGAAAGACGGATTTAATAAGATTTCCTTAACAGGATGCGATGCCCGTATTTTCTGCTTAGGGGTTCGTATTGTGAAGAAACGCACTGTGCAACAGATTCTCAATCTGATTCCCAAGGAGTCTGATGGTGAAAGTTTTGAAGATGCGCTTGCTCGTGTGTGTCGTGTTGTTGGTGGTGGGACTGCAATGGATAATGATGATAGTGACAGTGATTTGGAAGTTGTTGCAGATTCTTTTACTGTCAATCTACGTTGTCCGATGAGTGGTTCAAGAATGAAGGTTGCTGGAAGATTCAAGCCCTGCCCTCACATTGGCTGTTTTGATCTTGACGTTTTTGTGGAAATGAATCAACGTTCAAGGAAGGCAAGCTTCTATGCAAATTGCAAAGTGAAAGATGCATGGCAATGCCCTATTTGTCTTAAGAACTATGCGCTGGAGAATGTCATTATTGACCCTTATTTCAACCGTATCACTTCAAAGATGAGGTATTGTGAAGAGGATGTAGCAGAGATTGAGGTGAAGCCTGATGGTTCTTGGCGAGTTAAGACTAAAAGTGAAAGTGATCGTAGGGAGCTTGGGGAACTTGGACGGTGGCACCTTCCTGATAGCACACTTAGTGCCCCAACAGAAGGAGAAAGTATCCCAAAGACGGAAGTGTTGAAGCAGGTCAAACTGGAAGGTGTTTCAGAAGGTCGTACTGGATTGAAACTTGGAATCAGGAAGAATCACAACGGCTTTTGGGAATTCAGCAAACCTGAAGATATGAACACTTCTTCTGGTAATAGAATACAAGAGCAGTTTGCAGACCACGAGCTAAAAGTTATCCCAATGAGCAGCAGTGCCACTGGAAGTGGTAGGGATGGTGAAGATGCGAGTGTAAATCAGGATGGTGGTGGGAACTTTGATTTCTCTACTAACAATGGGATTGAGATGGATTCTTTTTCTCTGAATGCGTATGGATTTTCGGGACAAAATCCTTCTGCACCAGTCGGAGACGCAGAAGTTGTTTTACTTAGTGATTCTGATGACGATATAATGCCCTCTGGAACCATATATAGGAATGACAGAACTGATGCTTCTGGGATTAGTTTTCCTGCGGCGCCTTCTGGAATTGCTGATTCATTTGGTGGCGATCCGACGCTTGGAAATGGTGGGAACTCATGCATGGATCTTTTTGGCAATGATGATGAATTTATTCCTCTCTGGTCATCATTACCTCCCGGAACTCAATCTGGCCCTGGGTTCCAGTTATTTACTTCTGAGGCAGATGTACCAGATACCCTAGGTGGTCTGCAGCATGGTTCCATCCATTGTTCCACATCTATGAATGGATACACTCTGGCTCCAGAAACTACCATGGGATCTGCTACCCTATTACCTGATTCGTCTGTTGCACGTTCAGATATTGACATGAACGGTGGCTTAGTTGATTATCCAATGGTATTTCCTGGTGATGACCCTTCTCTTCAAATTTTTCTTCCCACAAGACCATCGGATGCATCAGTTCATTCTGATTTGAGAGATCAGGTTGATATGTCAAATGGTGTCCACACCGATGATTGGATATCTCTTAGGCTTGGGGGCGACGCCAGTGGCATCAATGGCACACCTGCAACTCCTAATGGTTTGAATTCTAGAGTGCAAATGCCATCCAGAGAAGGTGCCATGGATTCTTTGGCTGATACAGCTTCTTTGCTTCTCGGTGTGAATGATGGTAGCAGATCTGACAGAAGTAGGCGAAGATCAAATAGTCCTTTCTCATTTCCTCGCCAAAAACGTTCTGCGAGACCGCGATTGTTTCTTTCTATTGACTCGGACTCTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0001101 GO:0002682 GO:0002683 GO:0002831 GO:0002832 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006807 GO:0006935 GO:0006950 GO:0007154 GO:0007275 GO:0008150 GO:0008152 GO:0009266 GO:0009267 GO:0009408 GO:0009409 GO:0009414 GO:0009415 GO:0009553 GO:0009593 GO:0009605 GO:0009628 GO:0009653 GO:0009787 GO:0009791 GO:0009826 GO:0009856 GO:0009908 GO:0009909 GO:0009910 GO:0009966 GO:0009987 GO:0009991 GO:0010035 GO:0010112 GO:0010113 GO:0010183 GO:0010247 GO:0010286 GO:0010337 GO:0010565 GO:0010646 GO:0010817 GO:0016036 GO:0016043 GO:0016049 GO:0016740 GO:0016925 GO:0018193 GO:0018205 GO:0019222 GO:0019538 GO:0019787 GO:0019789 GO:0022414 GO:0023051 GO:0031323 GO:0031347 GO:0031348 GO:0031667 GO:0031668 GO:0031669 GO:0032101 GO:0032102 GO:0032350 GO:0032446 GO:0032501 GO:0032502 GO:0032989 GO:0033554 GO:0035670 GO:0036211 GO:0040007 GO:0040008 GO:0040011 GO:0042221 GO:0042330 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043900 GO:0043901 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044706 GO:0045088 GO:0045824 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048519 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048589 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048868 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050826 GO:0050896 GO:0050918 GO:0051093 GO:0051171 GO:0051239 GO:0051241 GO:0051301 GO:0051606 GO:0051704 GO:0051716 GO:0060560 GO:0061458 GO:0061659 GO:0061665 GO:0062012 GO:0065007 GO:0065008 GO:0070647 GO:0071496 GO:0071704 GO:0071840 GO:0080134 GO:0090352 GO:0090567 GO:0099402 GO:0140096 GO:1901419 GO:1901564 GO:1901700 GO:1903314 GO:1905957 GO:2000026 GO:2000070 GO:2000241 GO:2000242
KEGG Pathways
Metabolic & Signaling
ko04120 ko04630 ko05160 map04120 map04630 map05160
Pfam Domains
Protein Families
PHD SAP zf-MIZ

Protein Analysis

887

Amino Acids

97.17

Weight (kDa)

4.98

Isoelectric Point (pI)

39.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAP PF02037 14 - 44 8.7e-08 SAP domain
zf-MIZ PF02891 359 - 419 1.2e-14 MIZ/SP-RING zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2452
Acc36I ACCTGC 2 cut(s) 1489, 2452
AccB1I GGYRCC 4 cut(s) 239, 1428, 1933, 2495
AccB7I CCANNNNNTGG 1 cut(s) 581
AccI GTMKAC 1 cut(s) 496
AccII CGCG 1 cut(s) 2626
AciI CCGC 5 cut(s) 197, 351, 722, 1931, 2624
AclI AACGTT 2 cut(s) 1186, 2611
AclWI GGATC 8 cut(s) 114, 308, 321, 386, 506, 1961, 2010, 2203
AcoI YGGCCR 1 cut(s) 719
AcsI RAATTY 6 cut(s) 305, 355, 1577, 2024, 2304, 2464
AcuI CTGAAG 1 cut(s) 1611
AcyI GRCGYC 2 cut(s) 1934, 2421
AdeI CACNNNGTG 1 cut(s) 1451
AfaI GTAC 2 cut(s) 1531, 2106
AfeI AGCGCT 1 cut(s) 129
AfiI CCNNNNNNNGG 9 cut(s) 452, 581, 1097, 1145, 1463, 1483, 1890, 1976, 2394
AflII CTTAAG 1 cut(s) 1253
AhdI GACNNNNNGTC 1 cut(s) 646
AjnI CCWGG 3 cut(s) 775, 2071, 2281
AjuI GAANNNNNNNTTGG 2 cut(s) 568, 600
AluBI AGCT 6 cut(s) 14, 55, 1203, 1408, 1648, 2522
AluI AGCT 6 cut(s) 14, 55, 1203, 1408, 1648, 2522
Alw21I GWGCWC 1 cut(s) 57
Alw26I GTCTC 4 cut(s) 350, 359, 1821, 2614
AlwI GGATC 8 cut(s) 114, 308, 321, 386, 506, 1961, 2010, 2203
AlwNI CAGNNNCTG 1 cut(s) 470
Aor51HI AGCGCT 1 cut(s) 129
AoxI GGCC 2 cut(s) 719, 2068
ApeKI GCWGC 2 cut(s) 1668, 2128
ApoI RAATTY 6 cut(s) 305, 355, 1577, 2024, 2304, 2464
Asp700I GAANNNNTTC 1 cut(s) 2028
AspA2I CCTAGG 1 cut(s) 2116
AspLEI GCGC 4 cut(s) 130, 982, 1267, 1936
AspS9I GGNCC 2 cut(s) 815, 2069
AsuC2I CCSGG 1 cut(s) 2055
AsuHPI GGTGA 5 cut(s) 61, 974, 1363, 1709, 2297
AsuII TTCGAA 3 cut(s) 43, 86, 587
AvaII GGWCC 1 cut(s) 815
AvrII CCTAGG 1 cut(s) 2116
BaeGI GKGCMC 2 cut(s) 244, 1456
BamHI GGATCC 1 cut(s) 313
BanI GGYRCC 4 cut(s) 239, 1428, 1933, 2495
BanII GRGCYC 1 cut(s) 57
BarI GAAGNNNNNNTAC 2 cut(s) 1827, 1859
BauI CACGAG 2 cut(s) 987, 1643
Bbv12I GWGCWC 1 cut(s) 57
BbvI GCAGC 2 cut(s) 1680, 2140
BceAI ACGGC 1 cut(s) 1582
BcgI CGANNNNNNTGC 2 cut(s) 577, 611
BciT130I CCWGG 3 cut(s) 777, 2073, 2283
BciVI GTATCC 2 cut(s) 1484, 2159
BcnI CCSGG 1 cut(s) 2055
BcoDI GTCTC 4 cut(s) 350, 359, 1821, 2614
BfaI CTAG 4 cut(s) 15, 2117, 2469, 2659
BfmI CTRYAG 4 cut(s) 217, 737, 769, 2126
BfoI RGCGCY 2 cut(s) 131, 1937
BfrI CTTAAG 1 cut(s) 1253
BfuAI ACCTGC 2 cut(s) 1489, 2452
BfuI GTATCC 2 cut(s) 1484, 2159
BglII AGATCT 2 cut(s) 733, 2555
BisI GCNGC 4 cut(s) 722, 1669, 1932, 2129
BlnI CCTAGG 1 cut(s) 2116
BlsI GCNGC 4 cut(s) 723, 1670, 1933, 2130
Bme1390I CCNGG 4 cut(s) 777, 2055, 2073, 2283
Bme18I GGWCC 1 cut(s) 815
BmeRI GACNNNNNGTC 1 cut(s) 646
BmgT120I GGNCC 2 cut(s) 815, 2069
BmrFI CCNGG 4 cut(s) 777, 2055, 2073, 2283
BpmI CTGGAG 2 cut(s) 1289, 2163
Bpu10I CCTNAGC 1 cut(s) 892
Bpu14I TTCGAA 3 cut(s) 43, 86, 587
BpuMI CCSGG 1 cut(s) 2055
BsaHI GRCGYC 2 cut(s) 1934, 2421
BsaI GGTCTC 1 cut(s) 2614
BsaJI CCNNGG 6 cut(s) 948, 2071, 2072, 2116, 2190, 2499
BsaWI WCCGGW 1 cut(s) 117
BsaXI ACNNNNNCTCC 2 cut(s) 2161, 2191
Bsc4I CCNNNNNNNGG 9 cut(s) 452, 581, 1097, 1145, 1463, 1483, 1890, 1976, 2394
Bse1I ACTGG 8 cut(s) 464, 593, 1512, 1537, 1684, 1820, 2080, 2424
Bse3DI GCAATG 4 cut(s) 722, 1026, 1244, 2020
BseBI CCWGG 3 cut(s) 777, 2073, 2283
BseDI CCNNGG 6 cut(s) 948, 2071, 2072, 2116, 2190, 2499
BseGI GGATG 9 cut(s) 535, 717, 875, 1339, 1699, 1726, 2139, 2335, 2483
BseLI CCNNNNNNNGG 9 cut(s) 452, 581, 1097, 1145, 1463, 1483, 1890, 1976, 2394
BseMI GCAATG 4 cut(s) 722, 1026, 1244, 2020
BseMII CTCAG 3 cut(s) 339, 462, 2085
BseNI ACTGG 8 cut(s) 464, 593, 1512, 1537, 1684, 1820, 2080, 2424
BseSI GKGCMC 2 cut(s) 244, 1456
BseXI GCAGC 2 cut(s) 1680, 2140
BsgI GTGCAG 1 cut(s) 1800
Bsh1236I CGCG 1 cut(s) 2626
BshFI GGCC 2 cut(s) 721, 2070
BshNI GGYRCC 4 cut(s) 239, 1428, 1933, 2495
BsiHKAI GWGCWC 1 cut(s) 57
BsiSI CCGG 2 cut(s) 118, 2055
BslFI GGGAC 3 cut(s) 276, 1027, 1815
BslI CCNNNNNNNGG 9 cut(s) 452, 581, 1097, 1145, 1463, 1483, 1890, 1976, 2394
BsmAI GTCTC 4 cut(s) 350, 359, 1821, 2614
BsmBI CGTCTC 1 cut(s) 1821
BsmFI GGGAC 3 cut(s) 276, 1027, 1815
BsmI GAATGC 4 cut(s) 178, 565, 704, 1789
BsnI GGCC 2 cut(s) 721, 2070
Bso31I GGTCTC 1 cut(s) 2614
Bsp119I TTCGAA 3 cut(s) 43, 86, 587
Bsp1286I GDGCHC 3 cut(s) 57, 244, 1456
Bsp19I CCATGG 2 cut(s) 2190, 2499
BspACI CCGC 5 cut(s) 197, 351, 722, 1931, 2624
BspANI GGCC 2 cut(s) 721, 2070
BspCNI CTCAG 3 cut(s) 338, 463, 2086
BspFNI CGCG 1 cut(s) 2626
BspMAI CTGCAG 1 cut(s) 2130
BspMI ACCTGC 2 cut(s) 1489, 2452
BspPI GGATC 8 cut(s) 114, 308, 321, 386, 506, 1961, 2010, 2203
BspT104I TTCGAA 3 cut(s) 43, 86, 587
BspT107I GGYRCC 4 cut(s) 239, 1428, 1933, 2495
BspTI CTTAAG 1 cut(s) 1253
BspTNI GGTCTC 1 cut(s) 2614
BsrDI GCAATG 4 cut(s) 722, 1026, 1244, 2020
BsrI ACTGG 8 cut(s) 464, 593, 1512, 1537, 1684, 1820, 2080, 2424
BssECI CCNNGG 6 cut(s) 948, 2071, 2072, 2116, 2190, 2499
BssNI GRCGYC 2 cut(s) 1934, 2421
BssSI CACGAG 2 cut(s) 987, 1643
BssT1I CCWWGG 4 cut(s) 948, 2116, 2190, 2499
Bst2BI CACGAG 2 cut(s) 987, 1643
Bst2UI CCWGG 3 cut(s) 777, 2073, 2283
Bst4CI ACNGT 6 cut(s) 922, 1043, 1075, 1301, 1425, 2252
Bst6I CTCTTC 2 cut(s) 1323, 2304
BstACI GRCGYC 2 cut(s) 1934, 2421
BstAFI CTTAAG 1 cut(s) 1253
BstBI TTCGAA 3 cut(s) 43, 86, 587
BstC8I GCNNGC 2 cut(s) 984, 1201
BstDEI CTNAG 9 cut(s) 325, 471, 500, 892, 1448, 1847, 2094, 2257, 2405
BstDSI CCRYGG 2 cut(s) 2190, 2499
BstENI CCTNNNNNAGG 1 cut(s) 450
BstF5I GGATG 9 cut(s) 535, 717, 875, 1339, 1699, 1726, 2139, 2335, 2483
BstFNI CGCG 1 cut(s) 2626
BstH2I RGCGCY 2 cut(s) 131, 1937
BstHHI GCGC 4 cut(s) 130, 982, 1267, 1936
BstMAI GTCTC 4 cut(s) 350, 359, 1821, 2614
BstMWI GCNNNNNNNGC 4 cut(s) 348, 721, 2436, 2519
BstNI CCWGG 3 cut(s) 777, 2073, 2283
BstNSI RCATGY 1 cut(s) 731
BstSCI CCNGG 4 cut(s) 775, 2053, 2071, 2281
BstSFI CTRYAG 4 cut(s) 217, 737, 769, 2126
BstSLI GKGCMC 2 cut(s) 244, 1456
BstUI CGCG 1 cut(s) 2626
BstV1I GCAGC 2 cut(s) 1680, 2140
BstX2I RGATCY 7 cut(s) 106, 313, 391, 733, 2002, 2195, 2555
BstXI CCANNNNNNTGG 1 cut(s) 791
BstYI RGATCY 7 cut(s) 106, 313, 391, 733, 2002, 2195, 2555
BsuI GTATCC 2 cut(s) 1484, 2159
BsuRI GGCC 2 cut(s) 721, 2070
BtgI CCRYGG 2 cut(s) 2190, 2499
BtgZI GCGATG 1 cut(s) 888
BtsCI GGATG 9 cut(s) 535, 717, 875, 1339, 1699, 1726, 2139, 2335, 2483
BtsI GCAGTG 1 cut(s) 1678
BtsIMutI CAGTG 6 cut(s) 600, 918, 1048, 1677, 1678, 2431
BveI ACCTGC 2 cut(s) 1489, 2452
Cac8I GCNNGC 2 cut(s) 984, 1201
CaiI CAGNNNCTG 1 cut(s) 470
CfoI GCGC 4 cut(s) 130, 982, 1267, 1936
Cfr13I GGNCC 2 cut(s) 815, 2069
CseI GACGC 3 cut(s) 1838, 1981, 2429
Csp6I GTAC 2 cut(s) 1530, 2105
CviAII CATG 9 cut(s) 418, 728, 1233, 1995, 1999, 2132, 2191, 2245, 2500
CviQI GTAC 2 cut(s) 1530, 2105
DdeI CTNAG 9 cut(s) 325, 471, 500, 892, 1448, 1847, 2094, 2257, 2405
DinI GGCGCC 1 cut(s) 1935
DraIII CACNNNGTG 1 cut(s) 1451
DriI GACNNNNNGTC 1 cut(s) 646
EaeI YGGCCR 1 cut(s) 719
Eam1104I CTCTTC 2 cut(s) 1323, 2304
Eam1105I GACNNNNNGTC 1 cut(s) 646
EarI CTCTTC 2 cut(s) 1323, 2304
EciI GGCGGA 1 cut(s) 212
Ecl136II GAGCTC 1 cut(s) 55
Eco130I CCWWGG 4 cut(s) 948, 2116, 2190, 2499
Eco24I GRGCYC 1 cut(s) 57
Eco31I GGTCTC 1 cut(s) 2614
Eco32I GATATC 1 cut(s) 2400
Eco47I GGWCC 1 cut(s) 815
Eco47III AGCGCT 1 cut(s) 129
Eco53kI GAGCTC 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 1611
EcoICRI GAGCTC 1 cut(s) 55
EcoNI CCTNNNNNAGG 1 cut(s) 450
EcoRI GAATTC 3 cut(s) 355, 1577, 2464
EcoRII CCWGG 3 cut(s) 775, 2071, 2281
EcoRV GATATC 1 cut(s) 2400
EcoT14I CCWWGG 4 cut(s) 948, 2116, 2190, 2499
EcoT22I ATGCAT 3 cut(s) 1234, 2000, 2335
EcoT38I GRGCYC 1 cut(s) 57
EgeI GGCGCC 1 cut(s) 1935
EheI GGCGCC 1 cut(s) 1935
ErhI CCWWGG 4 cut(s) 948, 2116, 2190, 2499
Esp3I CGTCTC 1 cut(s) 1821
FaeI CATG 9 cut(s) 421, 731, 1236, 1998, 2002, 2135, 2194, 2248, 2503
FalI AAGNNNNNCTT 6 cut(s) 845, 877, 966, 998, 1187, 1219
FaqI GGGAC 3 cut(s) 276, 1027, 1815
FatI CATG 9 cut(s) 417, 727, 1232, 1994, 1998, 2131, 2190, 2244, 2499
FauI CCCGC 1 cut(s) 344
FblI GTMKAC 1 cut(s) 496
Fnu4HI GCNGC 4 cut(s) 722, 1669, 1932, 2129
FokI GGATG 9 cut(s) 522, 724, 882, 1346, 1706, 1733, 2126, 2342, 2470
FriOI GRGCYC 1 cut(s) 57
Fsp4HI GCNGC 4 cut(s) 722, 1669, 1932, 2129
FspBI CTAG 4 cut(s) 15, 2117, 2469, 2659
GlaI GCGC 4 cut(s) 129, 981, 1266, 1935
GluI GCNGC 4 cut(s) 722, 1669, 1932, 2129
GsuI CTGGAG 2 cut(s) 1289, 2163
HaeII RGCGCY 2 cut(s) 131, 1937
HaeIII GGCC 2 cut(s) 721, 2070
HapII CCGG 2 cut(s) 118, 2055
HgaI GACGC 3 cut(s) 1838, 1981, 2429
HhaI GCGC 4 cut(s) 130, 982, 1267, 1936
Hin1I GRCGYC 2 cut(s) 1934, 2421
Hin1II CATG 9 cut(s) 421, 731, 1236, 1998, 2002, 2135, 2194, 2248, 2503
Hin6I GCGC 4 cut(s) 128, 980, 1265, 1934
HinP1I GCGC 4 cut(s) 128, 980, 1265, 1934
HincII GTYRAC 2 cut(s) 115, 497
HindII GTYRAC 2 cut(s) 115, 497
HindIII AAGCTT 1 cut(s) 1201
HpaII CCGG 2 cut(s) 118, 2055
HphI GGTGA 5 cut(s) 61, 974, 1363, 1709, 2297
Hpy166II GTNNAC 4 cut(s) 115, 488, 497, 2383
Hpy8I GTNNAC 4 cut(s) 115, 488, 497, 2383
Hpy99I CGWCG 2 cut(s) 1975, 2423
HpyCH4III ACNGT 6 cut(s) 922, 1043, 1075, 1301, 1425, 2252
HpyCH4IV ACGT 5 cut(s) 1084, 1164, 1186, 2230, 2611
HpyF10VI GCNNNNNNNGC 4 cut(s) 348, 721, 2436, 2519
HpyF3I CTNAG 9 cut(s) 325, 471, 500, 892, 1448, 1847, 2094, 2257, 2405
HpySE526I ACGT 5 cut(s) 1084, 1164, 1186, 2230, 2611
Hsp92I GRCGYC 2 cut(s) 1934, 2421
Hsp92II CATG 9 cut(s) 421, 731, 1236, 1998, 2002, 2135, 2194, 2248, 2503
HspAI GCGC 4 cut(s) 128, 980, 1265, 1934
KasI GGCGCC 1 cut(s) 1933
LmnI GCTCC 3 cut(s) 785, 1405, 2182
Lsp1109I GCAGC 2 cut(s) 1680, 2140
MaeI CTAG 4 cut(s) 15, 2117, 2469, 2659
MaeII ACGT 5 cut(s) 1084, 1164, 1186, 2230, 2611
MaeIII GTNAC 2 cut(s) 523, 1037
MflI RGATCY 7 cut(s) 106, 313, 391, 733, 2002, 2195, 2555
MhlI GDGCHC 3 cut(s) 57, 244, 1456
Mly113I GGCGCC 1 cut(s) 1934
MlyI GAGTC 4 cut(s) 380, 962, 2639, 2645
MmeI TCCRAC 4 cut(s) 160, 599, 1804, 1994
Mph1103I ATGCAT 3 cut(s) 1234, 2000, 2335
MroXI GAANNNNTTC 1 cut(s) 2028
MseI TTAA 6 cut(s) 381, 552, 849, 863, 1254, 1377
MslI CAYNNNNRTG 3 cut(s) 1237, 2156, 2388
MspCI CTTAAG 1 cut(s) 1253
MspI CCGG 2 cut(s) 118, 2055
MspR9I CCNGG 4 cut(s) 777, 2055, 2073, 2283
Mva1269I GAATGC 4 cut(s) 178, 565, 704, 1789
MvaI CCWGG 3 cut(s) 777, 2073, 2283
MvnI CGCG 1 cut(s) 2626
MwoI GCNNNNNNNGC 4 cut(s) 348, 721, 2436, 2519
NarI GGCGCC 1 cut(s) 1934
NciI CCSGG 1 cut(s) 2055
NcoI CCATGG 2 cut(s) 2190, 2499
NlaIII CATG 9 cut(s) 421, 731, 1236, 1998, 2002, 2135, 2194, 2248, 2503
NmuCI GTSAC 1 cut(s) 1037
NsiI ATGCAT 3 cut(s) 1234, 2000, 2335
NspI RCATGY 1 cut(s) 731
NspV TTCGAA 3 cut(s) 43, 86, 587
PaqCI CACCTGC 1 cut(s) 2452
PasI CCCWGGG 1 cut(s) 2072
PcsI WCGNNNNNNNCGW 2 cut(s) 994, 2608
PctI GAATGC 4 cut(s) 178, 565, 704, 1789
PdmI GAANNNNTTC 1 cut(s) 2028
PflFI GACNNNGTC 2 cut(s) 370, 501
PflMI CCANNNNNTGG 1 cut(s) 581
PfoI TCCNGGA 1 cut(s) 2053
PkrI GCNGC 4 cut(s) 723, 1670, 1933, 2130
PleI GAGTC 4 cut(s) 379, 961, 2639, 2645
PluTI GGCGCC 1 cut(s) 1937
PpsI GAGTC 4 cut(s) 379, 961, 2639, 2645
Psp124BI GAGCTC 1 cut(s) 57
Psp1406I AACGTT 2 cut(s) 1186, 2611
Psp6I CCWGG 3 cut(s) 775, 2071, 2281
PspGI CCWGG 3 cut(s) 775, 2071, 2281
PspPI GGNCC 2 cut(s) 815, 2069
PstI CTGCAG 1 cut(s) 2130
PstNI CAGNNNCTG 1 cut(s) 470
PsuI RGATCY 7 cut(s) 106, 313, 391, 733, 2002, 2195, 2555
PsyI GACNNNGTC 2 cut(s) 370, 501
RsaI GTAC 2 cut(s) 1531, 2106
RsaNI GTAC 2 cut(s) 1530, 2105
RseI CAYNNNNRTG 3 cut(s) 1237, 2156, 2388
SacI GAGCTC 1 cut(s) 57
SalI GTCGAC 1 cut(s) 495
SaqAI TTAA 6 cut(s) 381, 552, 849, 863, 1254, 1377
SatI GCNGC 4 cut(s) 722, 1669, 1932, 2129
Sau96I GGNCC 2 cut(s) 815, 2069
SchI GAGTC 4 cut(s) 380, 962, 2639, 2645
ScrFI CCNGG 4 cut(s) 777, 2055, 2073, 2283
SduI GDGCHC 3 cut(s) 57, 244, 1456
SfcI CTRYAG 4 cut(s) 217, 737, 769, 2126
SfoI GGCGCC 1 cut(s) 1935
SfuI TTCGAA 3 cut(s) 43, 86, 587
SinI GGWCC 1 cut(s) 815
SmiMI CAYNNNNRTG 3 cut(s) 1237, 2156, 2388
SmlI CTYRAG 1 cut(s) 1253
SmoI CTYRAG 1 cut(s) 1253
SsiI CCGC 5 cut(s) 197, 351, 722, 1931, 2624
SspDI GGCGCC 1 cut(s) 1933
SspI AATATT 1 cut(s) 571
SspMI CTAG 4 cut(s) 15, 2117, 2469, 2659
SstI GAGCTC 1 cut(s) 57
StyD4I CCNGG 4 cut(s) 775, 2053, 2071, 2281
StyI CCWWGG 4 cut(s) 948, 2116, 2190, 2499
TaaI ACNGT 6 cut(s) 922, 1043, 1075, 1301, 1425, 2252
TaiI ACGT 5 cut(s) 1087, 1167, 1189, 2233, 2614
TaqI TCGA 4 cut(s) 43, 86, 496, 587
TauI GCSGC 2 cut(s) 724, 1934
Tru1I TTAA 6 cut(s) 381, 552, 849, 863, 1254, 1377
Tru9I TTAA 6 cut(s) 381, 552, 849, 863, 1254, 1377
TscAI CASTG 6 cut(s) 600, 925, 1048, 1678, 1684, 2431
TseFI GTSAC 1 cut(s) 1037
TseI GCWGC 2 cut(s) 1668, 2128
Tsp45I GTSAC 1 cut(s) 1037
TspGWI ACGGA 3 cut(s) 504, 858, 1499
TspRI CASTG 6 cut(s) 600, 925, 1048, 1678, 1684, 2431
Tth111I GACNNNGTC 2 cut(s) 370, 501
Van91I CCANNNNNTGG 1 cut(s) 581
Vha464I CTTAAG 1 cut(s) 1253
VpaK11BI GGWCC 1 cut(s) 815
XagI CCTNNNNNAGG 1 cut(s) 450
XapI RAATTY 6 cut(s) 305, 355, 1577, 2024, 2304, 2464
XbaI TCTAGA 1 cut(s) 2468
XceI RCATGY 1 cut(s) 731
XcmI CCANNNNNNNNNTGG 1 cut(s) 2506
XmaJI CCTAGG 1 cut(s) 2116
XmiI GTMKAC 1 cut(s) 496
XmnI GAANNNNTTC 1 cut(s) 2028
XspI CTAG 4 cut(s) 15, 2117, 2469, 2659
Zsp2I ATGCAT 3 cut(s) 1234, 2000, 2335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.