MD11G1131700.v1.1

E3 SUMO-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0005389
Physical Location & Seq
Forward (+)
11920102 .. 11931932
11831 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1131700.v1.1.491

Sequence Viewer

Length: 2634 bp
ATGGATTTGGTAGCTAGTTGCAAGGAAAAATTGGCATATTTTCGAATAAAAGAGCTCAAAGATGTTCTCACTGAATTAGGTCTATCAAAGCAGGGGAAGAAGCAGGATCTTGTTGACCGGATATTGGCCATTCTTTCTGATGAACAAGTCTCTAAAATGTGGGCAAAAAAGAATGCAGTTGGAAAGGAACAGGTGGCGGAACTTGTAGATCACACCTATAGAAAAATGCAGATTTCCGGTGCCCCTGATTTAGCATCAAAGGGACAATGCATCTCTGATAGTAGTAATGTGAAAATAAGAGGCGAAATTGAGGATCCCTACCAGTCAGCTATCAAGGTTCGCTGTCTCTGTGGGAATTCATTAGAAACAGAGTCAATGATTAAGTGTGAAGATCCAAGATGCCAAGTGTGGCAACACATGGGTTGCGTTATAGTTCCAGAGAAACCTATGGAAGGCAATCCACCAGTTCCCGAATTATTTTATTGTGAACTCTGTCGACTAAGTCGGGCTGATCCGTTTTGGGTAACGATTCAACATCCTTTACAGCCTGTTAAGTTGCATGTTACAAATATTCCAGCTGATGGTTCGAATCCAGTGCAAAGTGTGGAGAAAACGTTTCAACTCACAAGGGCAGACAAGGACCTGATGTCTAAACAAGAATATGATGTTCAGGTTTGGTGTATGCTTTTAAATGACAAAGTTGCATTCAGGATGCAATGGCCGCAATATGCAGATCTACAGGTCAATGGTATGCCTGTTCGTGCAATCAATAGACCTGGCTCGCAACTTCTTGGAGCTAATGGTCGTGATGATGGTCCAATTATCACACCATATACGAAAGACGGATTTAATAAGATTTCCTTAACAGGATGCGATGCCCGCATTTTCTGCTTAGGGGTTCGTATTGTGAAGAGACGCACAGTGCAACAGATTCTCAACCTGATTCCCAAGGAGTCTGATGGTGAGGGTTTTGAAGATGCGCTTGCTCGTGTGTGTCGATGTGTTGGTGGTGGGACTGCTACGGATAATGATGATAGTGACAGTGATTTGGAAGTTGTTGCAGATTCTTTTACTGTCAATCTACGTTGTCCGATGAGTGGTTCAAGAATGAAGGTTGCTGGGAGATTCAAGCCCTGCCCTCACATGGGCTGTTTTGATCTTGACGTTTTTGTGGAAATGAATCAACGTTCAAGGAAGTGGCAATGCCCCATTTGTCTCAAGAACTATGCGCTGGAGAATATCATTATCGACCCTTATTTCAACCGTATCACTTCAAAGATGAGGTATTGTGGAGAGGATGTAGCAGAGATTGAGGTGAAGCCTGATGGGTCTTGGCGAGTTAAGACTAAAAGTGAAAGTGATCGCAGGGAGCTTGGGGAACTTGGTCAGTGGCACCTGCCTGATGGTACACTTAGTGTCCCGACAGAAGGAGAAAGTATCCCAAAAACGGAAGTGTTGAAGCAGGTCAAACAGGAAGGTGTTTCAGAAGCTCATACTGGATTGAAACTTGGAATCAGGAAGAATCGCAATGGCTTTTGGGAAGTCAGCAAACCTGAAGATATGAACACTTCTTCTGGTAATAGATTACAAGAGCAGTTTGCAGACCATGAGCTAAAAGTTATCCCAATGAGTAGCAGTGCCACTGGAAGTGGTAGGGATGGCGAAGATGCAAGTGTTAATCAGGATGGTGGTGGAAACTTTGATTTCTCAACTAACAATGGAATTGAGATGGATTCTCTTTCTCTGAATGTTGATTCAGCATATGGATTTTCAGGACAAAATCCTTCTGCACCAGTAGGAGACGCAGAAGTTATTGTACTAAGTGATTCTGATGATGATATAATGCCCTCTGGAACCATCTATATGAATGACAGAACTGATGCTGCTGGGCTTGGTTTTCCTGTGGCGCCTTCTGGAATTGCTGATTCATATGGTGAAGATCACACGCTTGGAACTGGTGGGAACTCATGCTTGGGTCTTTTTGGCAACGAAGATGAATTTATTCCACTCTGGCCATTAGCTCCTGGAACTCAATCTGGTGCCGGGTTCCAGTTATTTACTTCTGAGGCAGATGTACCAGATACCCTAGGTGGTCTGCAGCATAGTTCCATCAATTGTTCCACATCCATGAATGGATACACTTTGGCTCCAGAAACTACCATGGGATCTGCTGCCCTAGGACTTGATTCTTCTGTTGCACGTTCAGATGTTGACATGAATGGTGGCTTAGTTGATAACCCAATGGCATTTCCTGGTGATGACCCTTCTCTTCAAATATTTCTTCCCACAAGACCATCTGATGCATCAGTTCATTCTGATTTGAGAGATCAAGCTGATATGTCAAATGGTGTCCACACTGATGATTGGATATCTCTTAGGCTTGGGGGTGACGCCAGTGGCATCAATGGGGCGCCTGCAAGTCCAAACGGTTTGAATTCTAGAATGCAAATGCCATCCAGAGAAGGTGCCATGGATTCTTTGGCTGATACAGCTTCTTTGCTTCTCGGTATGAATGACGGTAGCAGATCTGACAGGACAAGTAGGCAAAGATCAAACAGTCCTTTCTCATTTCCTCGCCAAAAACGTTCTGTGAGACCGCGATTATATCTTTCTATTGACTCGGACTCTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000902 GO:0001101 GO:0002682 GO:0002683 GO:0002831 GO:0002832 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006807 GO:0006935 GO:0006950 GO:0007154 GO:0007275 GO:0008150 GO:0008152 GO:0009266 GO:0009267 GO:0009408 GO:0009409 GO:0009414 GO:0009415 GO:0009553 GO:0009593 GO:0009605 GO:0009628 GO:0009653 GO:0009787 GO:0009791 GO:0009826 GO:0009856 GO:0009908 GO:0009909 GO:0009910 GO:0009966 GO:0009987 GO:0009991 GO:0010035 GO:0010112 GO:0010113 GO:0010183 GO:0010247 GO:0010286 GO:0010337 GO:0010565 GO:0010646 GO:0010817 GO:0016036 GO:0016043 GO:0016049 GO:0016740 GO:0016925 GO:0018193 GO:0018205 GO:0019222 GO:0019538 GO:0019787 GO:0019789 GO:0022414 GO:0023051 GO:0031323 GO:0031347 GO:0031348 GO:0031667 GO:0031668 GO:0031669 GO:0032101 GO:0032102 GO:0032350 GO:0032446 GO:0032501 GO:0032502 GO:0032989 GO:0033554 GO:0035670 GO:0036211 GO:0040007 GO:0040008 GO:0040011 GO:0042221 GO:0042330 GO:0042594 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043900 GO:0043901 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0044706 GO:0045088 GO:0045824 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048440 GO:0048467 GO:0048481 GO:0048519 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048589 GO:0048608 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0048868 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050826 GO:0050896 GO:0050918 GO:0051093 GO:0051171 GO:0051239 GO:0051241 GO:0051301 GO:0051606 GO:0051704 GO:0051716 GO:0060560 GO:0061458 GO:0061659 GO:0061665 GO:0062012 GO:0065007 GO:0065008 GO:0070647 GO:0071496 GO:0071704 GO:0071840 GO:0080134 GO:0090352 GO:0090567 GO:0099402 GO:0140096 GO:1901419 GO:1901564 GO:1901700 GO:1903314 GO:1905957 GO:2000026 GO:2000070 GO:2000241 GO:2000242
KEGG Pathways
Metabolic & Signaling
ko04120 ko04630 ko05160 map04120 map04630 map05160
Pfam Domains
Protein Families
PHD SAP zf-MIZ

Protein Analysis

878

Amino Acids

95.82

Weight (kDa)

4.91

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAP PF02037 14 - 42 1.1e-07 SAP domain
zf-MIZ PF02891 359 - 407 3.4e-19 MIZ/SP-RING zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1404
Acc36I ACCTGC 2 cut(s) 1404, 1453
AccB1I GGYRCC 6 cut(s) 239, 1392, 1906, 2039, 2410, 2465
AccB7I CCANNNNNTGG 1 cut(s) 581
AccI GTMKAC 1 cut(s) 496
AccII CGCG 1 cut(s) 2599
AciI CCGC 4 cut(s) 197, 722, 880, 2597
AclI AACGTT 3 cut(s) 614, 1186, 2584
AclWI GGATC 6 cut(s) 114, 308, 321, 386, 506, 2173
AcoI YGGCCR 3 cut(s) 126, 719, 2012
AcsI RAATTY 3 cut(s) 355, 1997, 2434
AcuI CTGAAG 1 cut(s) 1575
AcyI GRCGYC 3 cut(s) 1907, 2391, 2411
AdeI CACNNNGTG 1 cut(s) 1415
AfaI GTAC 3 cut(s) 1408, 1818, 2076
AfiI CCNNNNNNNGG 8 cut(s) 124, 452, 581, 1097, 1144, 1145, 1427, 1447
AhdI GACNNNNNGTC 1 cut(s) 646
AjnI CCWGG 3 cut(s) 775, 2023, 2251
AjuI GAANNNNNNNTTGG 2 cut(s) 1955, 1987
Alw21I GWGCWC 1 cut(s) 57
Alw26I GTCTC 6 cut(s) 154, 350, 907, 1220, 1794, 2587
AlwI GGATC 6 cut(s) 114, 308, 321, 386, 506, 2173
AoxI GGCC 3 cut(s) 126, 719, 2012
ApeKI GCWGC 3 cut(s) 1883, 2098, 2171
ApoI RAATTY 3 cut(s) 355, 1997, 2434
Asp700I GAANNNNTTC 1 cut(s) 2001
AspA2I CCTAGG 2 cut(s) 2086, 2176
AspLEI GCGC 4 cut(s) 982, 1231, 1909, 2413
AspS9I GGNCC 2 cut(s) 640, 815
AsuC2I CCSGG 1 cut(s) 2044
AsuHPI GGTGA 5 cut(s) 974, 1327, 1946, 2267, 2399
AsuII TTCGAA 2 cut(s) 43, 587
AvaII GGWCC 2 cut(s) 640, 815
AvrII CCTAGG 2 cut(s) 2086, 2176
BaeGI GKGCMC 1 cut(s) 244
BalI TGGCCA 2 cut(s) 128, 2014
BamHI GGATCC 1 cut(s) 313
BanI GGYRCC 6 cut(s) 239, 1392, 1906, 2039, 2410, 2465
BanII GRGCYC 1 cut(s) 57
BarI GAAGNNNNNNTAC 2 cut(s) 1800, 1832
BauI CACGAG 1 cut(s) 987
Bbv12I GWGCWC 1 cut(s) 57
BbvI GCAGC 3 cut(s) 1870, 2110, 2158
BcgI CGANNNNNNTGC 2 cut(s) 577, 611
BciT130I CCWGG 3 cut(s) 777, 2025, 2253
BciVI GTATCC 2 cut(s) 1448, 2129
BcnI CCSGG 1 cut(s) 2044
BcoDI GTCTC 6 cut(s) 154, 350, 907, 1220, 1794, 2587
BfaI CTAG 4 cut(s) 15, 2087, 2177, 2439
BfmI CTRYAG 3 cut(s) 217, 737, 2096
BfoI RGCGCY 2 cut(s) 1910, 2414
BfuAI ACCTGC 2 cut(s) 1404, 1453
BfuI GTATCC 2 cut(s) 1448, 2129
BglII AGATCT 2 cut(s) 733, 2525
BisI GCNGC 4 cut(s) 722, 1884, 2099, 2172
BlnI CCTAGG 2 cut(s) 2086, 2176
BlsI GCNGC 4 cut(s) 723, 1885, 2100, 2173
Bme1390I CCNGG 4 cut(s) 777, 2025, 2044, 2253
Bme18I GGWCC 2 cut(s) 640, 815
BmeRI GACNNNNNGTC 1 cut(s) 646
BmgT120I GGNCC 2 cut(s) 640, 815
BmrFI CCNGG 4 cut(s) 777, 2025, 2044, 2253
BpmI CTGGAG 2 cut(s) 1253, 2133
Bpu10I CCTNAGC 1 cut(s) 892
Bpu14I TTCGAA 2 cut(s) 43, 587
BpuEI CTTGAG 1 cut(s) 1202
BpuMI CCSGG 1 cut(s) 2044
BsaHI GRCGYC 3 cut(s) 1907, 2391, 2411
BsaI GGTCTC 1 cut(s) 2587
BsaJI CCNNGG 5 cut(s) 948, 2086, 2160, 2176, 2469
BsaWI WCCGGW 2 cut(s) 117, 236
BsaXI ACNNNNNCTCC 6 cut(s) 599, 629, 1284, 1314, 2131, 2161
Bsc4I CCNNNNNNNGG 8 cut(s) 124, 452, 581, 1097, 1144, 1145, 1427, 1447
Bse1I ACTGG 9 cut(s) 322, 464, 593, 1501, 1648, 1793, 1960, 2050, 2394
Bse3DI GCAATG 3 cut(s) 722, 1208, 1534
BseBI CCWGG 3 cut(s) 777, 2025, 2253
BseDI CCNNGG 5 cut(s) 948, 2086, 2160, 2176, 2469
BseGI GGATG 8 cut(s) 535, 717, 875, 1303, 1663, 1690, 2123, 2453
BseLI CCNNNNNNNGG 8 cut(s) 124, 452, 581, 1097, 1144, 1145, 1427, 1447
BseMI GCAATG 3 cut(s) 722, 1208, 1534
BseMII CTCAG 1 cut(s) 2055
BseNI ACTGG 9 cut(s) 322, 464, 593, 1501, 1648, 1793, 1960, 2050, 2394
BseSI GKGCMC 1 cut(s) 244
BseXI GCAGC 3 cut(s) 1870, 2110, 2158
BseYI CCCAGC 2 cut(s) 1118, 1886
BsgI GTGCAG 1 cut(s) 1773
Bsh1236I CGCG 1 cut(s) 2599
BshFI GGCC 3 cut(s) 128, 721, 2014
BshNI GGYRCC 6 cut(s) 239, 1392, 1906, 2039, 2410, 2465
BsiHKAI GWGCWC 1 cut(s) 57
BsiSI CCGG 3 cut(s) 118, 237, 2043
BslFI GGGAC 3 cut(s) 276, 1027, 1403
BslI CCNNNNNNNGG 8 cut(s) 124, 452, 581, 1097, 1144, 1145, 1427, 1447
BsmAI GTCTC 6 cut(s) 154, 350, 907, 1220, 1794, 2587
BsmBI CGTCTC 2 cut(s) 907, 1794
BsmFI GGGAC 3 cut(s) 276, 1027, 1403
BsmI GAATGC 3 cut(s) 178, 704, 2448
BsnI GGCC 3 cut(s) 128, 721, 2014
Bso31I GGTCTC 1 cut(s) 2587
Bsp119I TTCGAA 2 cut(s) 43, 587
Bsp1286I GDGCHC 2 cut(s) 57, 244
Bsp19I CCATGG 2 cut(s) 2160, 2469
BspACI CCGC 4 cut(s) 197, 722, 880, 2597
BspANI GGCC 3 cut(s) 128, 721, 2014
BspCNI CTCAG 1 cut(s) 2056
BspFNI CGCG 1 cut(s) 2599
BspMAI CTGCAG 1 cut(s) 2100
BspMI ACCTGC 2 cut(s) 1404, 1453
BspPI GGATC 6 cut(s) 114, 308, 321, 386, 506, 2173
BspT104I TTCGAA 2 cut(s) 43, 587
BspT107I GGYRCC 6 cut(s) 239, 1392, 1906, 2039, 2410, 2465
BspTNI GGTCTC 1 cut(s) 2587
BsrDI GCAATG 3 cut(s) 722, 1208, 1534
BsrI ACTGG 9 cut(s) 322, 464, 593, 1501, 1648, 1793, 1960, 2050, 2394
BssECI CCNNGG 5 cut(s) 948, 2086, 2160, 2176, 2469
BssNI GRCGYC 3 cut(s) 1907, 2391, 2411
BssSI CACGAG 1 cut(s) 987
BssT1I CCWWGG 5 cut(s) 948, 2086, 2160, 2176, 2469
Bst2BI CACGAG 1 cut(s) 987
Bst2UI CCWGG 3 cut(s) 777, 2025, 2253
Bst4CI ACNGT 7 cut(s) 922, 1043, 1075, 1265, 2429, 2519, 2558
Bst6I CTCTTC 2 cut(s) 905, 2274
BstACI GRCGYC 3 cut(s) 1907, 2391, 2411
BstAPI GCANNNNNTGC 1 cut(s) 888
BstBI TTCGAA 2 cut(s) 43, 587
BstC8I GCNNGC 4 cut(s) 782, 880, 984, 2415
BstDEI CTNAG 7 cut(s) 500, 892, 1412, 1820, 2064, 2227, 2375
BstDSI CCRYGG 2 cut(s) 2160, 2469
BstENI CCTNNNNNAGG 1 cut(s) 450
BstF5I GGATG 8 cut(s) 535, 717, 875, 1303, 1663, 1690, 2123, 2453
BstFNI CGCG 1 cut(s) 2599
BstH2I RGCGCY 2 cut(s) 1910, 2414
BstHHI GCGC 4 cut(s) 982, 1231, 1909, 2413
BstMAI GTCTC 6 cut(s) 154, 350, 907, 1220, 1794, 2587
BstMWI GCNNNNNNNGC 4 cut(s) 721, 879, 888, 2489
BstNI CCWGG 3 cut(s) 777, 2025, 2253
BstNSI RCATGY 1 cut(s) 563
BstSCI CCNGG 4 cut(s) 775, 2023, 2042, 2251
BstSFI CTRYAG 3 cut(s) 217, 737, 2096
BstSLI GKGCMC 1 cut(s) 244
BstUI CGCG 1 cut(s) 2599
BstV1I GCAGC 3 cut(s) 1870, 2110, 2158
BstX2I RGATCY 6 cut(s) 106, 313, 391, 733, 2165, 2525
BstYI RGATCY 6 cut(s) 106, 313, 391, 733, 2165, 2525
BsuI GTATCC 2 cut(s) 1448, 2129
BsuRI GGCC 3 cut(s) 128, 721, 2014
BtgI CCRYGG 2 cut(s) 2160, 2469
BtgZI GCGATG 1 cut(s) 888
BtsCI GGATG 8 cut(s) 535, 717, 875, 1303, 1663, 1690, 2123, 2453
BtsI GCAGTG 1 cut(s) 1642
BtsIMutI CAGTG 9 cut(s) 69, 600, 927, 1048, 1394, 1641, 1642, 2355, 2401
BveI ACCTGC 2 cut(s) 1404, 1453
Cac8I GCNNGC 4 cut(s) 782, 880, 984, 2415
CfoI GCGC 4 cut(s) 982, 1231, 1909, 2413
Cfr13I GGNCC 2 cut(s) 640, 815
CseI GACGC 3 cut(s) 924, 1811, 2399
Csp6I GTAC 3 cut(s) 1407, 1817, 2075
CviAII CATG 9 cut(s) 418, 560, 1144, 1607, 1968, 2128, 2161, 2215, 2470
CviQI GTAC 3 cut(s) 1407, 1817, 2075
DdeI CTNAG 7 cut(s) 500, 892, 1412, 1820, 2064, 2227, 2375
DinI GGCGCC 2 cut(s) 1908, 2412
DraI TTTAAA 1 cut(s) 690
DraIII CACNNNGTG 1 cut(s) 1415
DriI GACNNNNNGTC 1 cut(s) 646
EaeI YGGCCR 3 cut(s) 126, 719, 2012
Eam1104I CTCTTC 2 cut(s) 905, 2274
Eam1105I GACNNNNNGTC 1 cut(s) 646
EarI CTCTTC 2 cut(s) 905, 2274
EciI GGCGGA 1 cut(s) 212
Ecl136II GAGCTC 1 cut(s) 55
Eco130I CCWWGG 5 cut(s) 948, 2086, 2160, 2176, 2469
Eco24I GRGCYC 1 cut(s) 57
Eco31I GGTCTC 1 cut(s) 2587
Eco32I GATATC 1 cut(s) 2370
Eco47I GGWCC 2 cut(s) 640, 815
Eco53kI GAGCTC 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 1575
EcoICRI GAGCTC 1 cut(s) 55
EcoNI CCTNNNNNAGG 1 cut(s) 450
EcoO109I RGGNCCY 1 cut(s) 640
EcoRI GAATTC 2 cut(s) 355, 2434
EcoRII CCWGG 3 cut(s) 775, 2023, 2251
EcoRV GATATC 1 cut(s) 2370
EcoT14I CCWWGG 5 cut(s) 948, 2086, 2160, 2176, 2469
EcoT22I ATGCAT 2 cut(s) 272, 2305
EcoT38I GRGCYC 1 cut(s) 57
EgeI GGCGCC 2 cut(s) 1908, 2412
EheI GGCGCC 2 cut(s) 1908, 2412
ErhI CCWWGG 5 cut(s) 948, 2086, 2160, 2176, 2469
Esp3I CGTCTC 2 cut(s) 907, 1794
FaeI CATG 9 cut(s) 421, 563, 1147, 1610, 1971, 2131, 2164, 2218, 2473
FalI AAGNNNNNCTT 4 cut(s) 845, 877, 966, 998
FaqI GGGAC 3 cut(s) 276, 1027, 1403
FatI CATG 9 cut(s) 417, 559, 1143, 1606, 1967, 2127, 2160, 2214, 2469
FauI CCCGC 1 cut(s) 887
FauNDI CATATG 2 cut(s) 1762, 1930
FblI GTMKAC 1 cut(s) 496
Fnu4HI GCNGC 4 cut(s) 722, 1884, 2099, 2172
FokI GGATG 8 cut(s) 522, 724, 882, 1310, 1670, 1697, 2110, 2440
FriOI GRGCYC 1 cut(s) 57
Fsp4HI GCNGC 4 cut(s) 722, 1884, 2099, 2172
FspBI CTAG 4 cut(s) 15, 2087, 2177, 2439
GlaI GCGC 4 cut(s) 981, 1230, 1908, 2412
GluI GCNGC 4 cut(s) 722, 1884, 2099, 2172
GsaI CCCAGC 2 cut(s) 1122, 1890
GsuI CTGGAG 2 cut(s) 1253, 2133
HaeII RGCGCY 2 cut(s) 1910, 2414
HaeIII GGCC 3 cut(s) 128, 721, 2014
HapII CCGG 3 cut(s) 118, 237, 2043
HgaI GACGC 3 cut(s) 924, 1811, 2399
HhaI GCGC 4 cut(s) 982, 1231, 1909, 2413
Hin1I GRCGYC 3 cut(s) 1907, 2391, 2411
Hin1II CATG 9 cut(s) 421, 563, 1147, 1610, 1971, 2131, 2164, 2218, 2473
Hin6I GCGC 4 cut(s) 980, 1229, 1907, 2411
HinP1I GCGC 4 cut(s) 980, 1229, 1907, 2411
HincII GTYRAC 3 cut(s) 115, 497, 2212
HindII GTYRAC 3 cut(s) 115, 497, 2212
HpaII CCGG 3 cut(s) 118, 237, 2043
HphI GGTGA 5 cut(s) 974, 1327, 1946, 2267, 2399
Hpy166II GTNNAC 6 cut(s) 115, 488, 497, 1409, 2212, 2353
Hpy8I GTNNAC 6 cut(s) 115, 488, 497, 1409, 2212, 2353
HpyAV CCTTC 8 cut(s) 446, 1105, 1421, 1469, 1794, 1920, 2274, 2456
HpyCH4III ACNGT 7 cut(s) 922, 1043, 1075, 1265, 2429, 2519, 2558
HpyCH4IV ACGT 6 cut(s) 614, 1084, 1164, 1186, 2200, 2584
HpyF10VI GCNNNNNNNGC 4 cut(s) 721, 879, 888, 2489
HpyF3I CTNAG 7 cut(s) 500, 892, 1412, 1820, 2064, 2227, 2375
HpySE526I ACGT 6 cut(s) 614, 1084, 1164, 1186, 2200, 2584
Hsp92I GRCGYC 3 cut(s) 1907, 2391, 2411
Hsp92II CATG 9 cut(s) 421, 563, 1147, 1610, 1971, 2131, 2164, 2218, 2473
HspAI GCGC 4 cut(s) 980, 1229, 1907, 2411
KasI GGCGCC 2 cut(s) 1906, 2410
LmnI GCTCC 4 cut(s) 794, 1369, 2026, 2152
Lsp1109I GCAGC 3 cut(s) 1870, 2110, 2158
MaeI CTAG 4 cut(s) 15, 2087, 2177, 2439
MaeII ACGT 6 cut(s) 614, 1084, 1164, 1186, 2200, 2584
MaeIII GTNAC 4 cut(s) 523, 562, 1037, 2387
MfeI CAATTG 1 cut(s) 2113
MflI RGATCY 6 cut(s) 106, 313, 391, 733, 2165, 2525
MhlI GDGCHC 2 cut(s) 57, 244
MlsI TGGCCA 2 cut(s) 128, 2014
MluNI TGGCCA 2 cut(s) 128, 2014
Mly113I GGCGCC 2 cut(s) 1907, 2411
MlyI GAGTC 4 cut(s) 380, 962, 2612, 2618
MmeI TCCRAC 1 cut(s) 160
Mox20I TGGCCA 2 cut(s) 128, 2014
Mph1103I ATGCAT 2 cut(s) 272, 2305
MroXI GAANNNNTTC 1 cut(s) 2001
MscI TGGCCA 2 cut(s) 128, 2014
MseI TTAA 7 cut(s) 381, 552, 689, 849, 863, 1341, 1677
MslI CAYNNNNRTG 3 cut(s) 1862, 2126, 2358
Msp20I TGGCCA 2 cut(s) 128, 2014
MspA1I CMGCKG 1 cut(s) 578
MspI CCGG 3 cut(s) 118, 237, 2043
MspR9I CCNGG 4 cut(s) 777, 2025, 2044, 2253
MunI CAATTG 1 cut(s) 2113
Mva1269I GAATGC 3 cut(s) 178, 704, 2448
MvaI CCWGG 3 cut(s) 777, 2025, 2253
MvnI CGCG 1 cut(s) 2599
MwoI GCNNNNNNNGC 4 cut(s) 721, 879, 888, 2489
NarI GGCGCC 2 cut(s) 1907, 2411
NciI CCSGG 1 cut(s) 2044
NcoI CCATGG 2 cut(s) 2160, 2469
NdeI CATATG 2 cut(s) 1762, 1930
NlaIII CATG 9 cut(s) 421, 563, 1147, 1610, 1971, 2131, 2164, 2218, 2473
NmuCI GTSAC 2 cut(s) 1037, 2387
NsiI ATGCAT 2 cut(s) 272, 2305
NspI RCATGY 1 cut(s) 563
NspV TTCGAA 2 cut(s) 43, 587
PaqCI CACCTGC 1 cut(s) 1404
PcsI WCGNNNNNNNCGW 2 cut(s) 994, 2581
PctI GAATGC 3 cut(s) 178, 704, 2448
PdmI GAANNNNTTC 1 cut(s) 2001
PflFI GACNNNGTC 1 cut(s) 501
PflMI CCANNNNNTGG 1 cut(s) 581
PfoI TCCNGGA 1 cut(s) 2023
PkrI GCNGC 4 cut(s) 723, 1885, 2100, 2173
PleI GAGTC 4 cut(s) 379, 961, 2612, 2618
PluTI GGCGCC 2 cut(s) 1910, 2414
PpsI GAGTC 4 cut(s) 379, 961, 2612, 2618
PpuMI RGGWCCY 1 cut(s) 640
Psp124BI GAGCTC 1 cut(s) 57
Psp1406I AACGTT 3 cut(s) 614, 1186, 2584
Psp5II RGGWCCY 1 cut(s) 640
Psp6I CCWGG 3 cut(s) 775, 2023, 2251
PspFI CCCAGC 2 cut(s) 1118, 1886
PspGI CCWGG 3 cut(s) 775, 2023, 2251
PspPI GGNCC 2 cut(s) 640, 815
PspPPI RGGWCCY 1 cut(s) 640
PstI CTGCAG 1 cut(s) 2100
PsuI RGATCY 6 cut(s) 106, 313, 391, 733, 2165, 2525
PsyI GACNNNGTC 1 cut(s) 501
PvuII CAGCTG 1 cut(s) 578
RsaI GTAC 3 cut(s) 1408, 1818, 2076
RsaNI GTAC 3 cut(s) 1407, 1817, 2075
RseI CAYNNNNRTG 3 cut(s) 1862, 2126, 2358
SacI GAGCTC 1 cut(s) 57
SalI GTCGAC 1 cut(s) 495
SaqAI TTAA 7 cut(s) 381, 552, 689, 849, 863, 1341, 1677
SatI GCNGC 4 cut(s) 722, 1884, 2099, 2172
Sau96I GGNCC 2 cut(s) 640, 815
SchI GAGTC 4 cut(s) 380, 962, 2612, 2618
ScrFI CCNGG 4 cut(s) 777, 2025, 2044, 2253
SduI GDGCHC 2 cut(s) 57, 244
SfcI CTRYAG 3 cut(s) 217, 737, 2096
SfoI GGCGCC 2 cut(s) 1908, 2412
SfuI TTCGAA 2 cut(s) 43, 587
SinI GGWCC 2 cut(s) 640, 815
SmiMI CAYNNNNRTG 3 cut(s) 1862, 2126, 2358
SmlI CTYRAG 1 cut(s) 1217
SmoI CTYRAG 1 cut(s) 1217
SsiI CCGC 4 cut(s) 197, 722, 880, 2597
SspDI GGCGCC 2 cut(s) 1906, 2410
SspI AATATT 2 cut(s) 571, 2277
SspMI CTAG 4 cut(s) 15, 2087, 2177, 2439
SstI GAGCTC 1 cut(s) 57
StyD4I CCNGG 4 cut(s) 775, 2023, 2042, 2251
StyI CCWWGG 5 cut(s) 948, 2086, 2160, 2176, 2469
TaaI ACNGT 7 cut(s) 922, 1043, 1075, 1265, 2429, 2519, 2558
TaiI ACGT 6 cut(s) 617, 1087, 1167, 1189, 2203, 2587
TaqI TCGA 5 cut(s) 43, 496, 587, 997, 1248
TatI WGTACW 1 cut(s) 1816
TauI GCSGC 1 cut(s) 724
Tru1I TTAA 7 cut(s) 381, 552, 689, 849, 863, 1341, 1677
Tru9I TTAA 7 cut(s) 381, 552, 689, 849, 863, 1341, 1677
TscAI CASTG 9 cut(s) 76, 600, 927, 1048, 1394, 1642, 1648, 2362, 2401
TseFI GTSAC 2 cut(s) 1037, 2387
TseI GCWGC 3 cut(s) 1883, 2098, 2171
Tsp45I GTSAC 2 cut(s) 1037, 2387
TspGWI ACGGA 4 cut(s) 504, 858, 1037, 1463
TspRI CASTG 9 cut(s) 76, 600, 927, 1048, 1394, 1642, 1648, 2362, 2401
Tth111I GACNNNGTC 1 cut(s) 501
Van91I CCANNNNNTGG 1 cut(s) 581
VpaK11BI GGWCC 2 cut(s) 640, 815
XagI CCTNNNNNAGG 1 cut(s) 450
XapI RAATTY 3 cut(s) 355, 1997, 2434
XbaI TCTAGA 1 cut(s) 2438
XceI RCATGY 1 cut(s) 563
XcmI CCANNNNNNNNNTGG 1 cut(s) 2476
XmaJI CCTAGG 2 cut(s) 2086, 2176
XmiI GTMKAC 1 cut(s) 496
XmnI GAANNNNTTC 1 cut(s) 2001
XspI CTAG 4 cut(s) 15, 2087, 2177, 2439
Zsp2I ATGCAT 2 cut(s) 272, 2305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.