Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0008595

Physical Location & Seq

Genomic Coordinates Reverse (-)

16659061 .. 16667538

Gene Structure 8478 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1149800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1713 bp

ATGGCTATGGCTCAAGCGAAGCCTGCCGGCGGCGGCGGCGGGTGCCAAGGGCTGGAGTCCATGTACTCGATGATTGCGCTCGTCTTCATCCTCGTCGCCTGCGTTGAGCTATGCGACGCTGCCACCGTCGTCGACGTGTATCGGCTGATCCAGTACGACATCTCCGGTGCTCCTTTCGGATCTCGGCTCGCCAACCTCAACCACCATGCTGGCTCTTTGCACTTTGCTCCTGGCTCTGATCTCTCCCGCACTGTCCTCATCATCCCCTTGCGCGAACTCAACATCTCCTTCGTCACTGATTACATTACCCAGAAGCAGCCTCTGGGAGGTTTGCTGTTTTTGCTGCCCCAGATATTTAGTATTGAAAATAGAGAAAGGGCAACGAGTAATCATGAAACTGATGGAGAGGTGCCAATGAAGAATTATCTGGCCGAACTTGAAAAATTACTTATTCATTCCAAGATACCTTATCCTGTATATTTTGCTTTTGAAGATGATGATATTGAGGCTGTGTTGGCTGATGTCAAGCGGAATGATGTCTCTGGTCAGCCTGCTACTGCGACTACTGGCGGATACAAGCTTGTTGTTTCTGTGCCAGAACCCAAAAAGCGCGTATCACCCACCATCACAAACATTCAGGGATGGTTGTCAGGACTGAAAACAGATGGAGATGCAAATCAGCTTCCAACTATTGCTATAGTAGCATCATATGATACTTTTGGGGCAGCCCCTGCTTTATCAGTGGGAAGTGATAGCAATGGAAGCGGTGTTGTGGCACTTCTTGAGATTGCTAGGTTGTTTTCTGTTCTTTACTCCAATCCTAAGACAAGAGGAAGGTATAATTTACTTTTTGGGTTAACATCAGGAGGACCTTATAACTACAATGGAACTCATAAGTGGCTTCGGAGCTTTGATCAACGCCAGCGTGAGAGTATTGACTATGCTATTTGCTTGAATAGTATTGGCTCATGGGACAATGACTTGTGGATTCATGTGTCTAAGCCTCCAGAAAATGCCTACATAAAGCAAATTTTTGAAGGTTTCTCTAGTGTAGCAGAAGAACTGAGCCTTAACGTTGGTCTGAAGCACAAGAAGATAAATATTTCAAATCCGCGAGTAGCTTGGGAGCATGAACAGTTTTCACGGCTACGAGTTACTGCTGCCACTCTTTCTGGACTCCCTGTTGCTCCTGAACTATTGGAAAGCACTGGCAGTCTTGTTGATGGAAGACCTTTCGTGAATGAAACTGCAATCATCAGAAGTGTCAAGCTAGTTGCTGAGAGCCTTGCGAGGCATATCTATGGTCACGAAGGAAAGAACGTTCAAATCTTTGCAGACAACAGTAGTTTAGCAGTTAATCCTTCTTACATAAAATCGTGGTTGGATCTACTGTCACAAACACCTCGAGTGGCGCCATTTTTATCAAAGAATGACCCGTTTATCTTGGCGTTGAAGAAGGAATTGGAAGACAATACAGATGAGGTGAACGTGCAGCATGAGGTGCTTGACGGGATGTTTACTTTCTATGATTCAACTAGGGCTAAACTTAACGTATATCAGGTGGCTAGTGTGACATTCGACTTGCTGTTGCTTCTGGTGTTGGGATCATACTTGATTGTACTTTTCAGTTTCCTTGTCATCACAACTAGGGGTCTTGATGATCTAATCAGTTTATTTCGACGACCTCCCTCACGTAAAGTGAAAACAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006355 GO:0006357 GO:0007275 GO:0007399 GO:0008150 GO:0009889 GO:0009966 GO:0009968 GO:0010468 GO:0010556 GO:0010646 GO:0010648 GO:0012505 GO:0016020 GO:0019219 GO:0019222 GO:0023051 GO:0023057 GO:0031323 GO:0031326 GO:0031984 GO:0032501 GO:0032502 GO:0032925 GO:0032926 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048483 GO:0048484 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051171 GO:0051239 GO:0051241 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0090092 GO:0090101 GO:0098827 GO:1900107 GO:1900108 GO:1900145 GO:1900146 GO:1900175 GO:1900176 GO:1903506 GO:2000026 GO:2000112 GO:2000380 GO:2000381 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Nicastrin

Protein Analysis

571

Amino Acids

62.68

Weight (kDa)

5.99

Isoelectric Point (pI)

39.79

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nicastrin	PF05450	230 - 349	2.7e-07	Nicastrin large lobe

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008595)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44330
fragaria_vesca	FvH4_3g25670 FvH4_3g25670 FvH4_3g25670 FvH4_3g25670
malus_domestica	MD03G1149800.v1.1 MD11G1168600.v1.1
prunus_persica	Prupe.6G135800_v2.0.a1
pyrus_communis	pycom11g14130
rosa_chinensis	RchiOBHm_Chr5g0045881
rosa_laevigata	RLG00000034394
rosa_multiflora	Rmu_sc0001212.1_g000004
rosa_roxburghii	Rroxscaffold_1G00028740 Rroxscaffold_1G00035230 Rroxscaffold_2G00117520 Rroxscaffold_2G00117530
rosa_rugosa	Rorug05G0226200 Rorug05G0226300
rosa_samantha	Rh5AG307700 Rh5BG316000 Rh5CG342200 Rh5DG326300
rosa_wichuraiana	Rw5G028810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	876
AccB1I	GGYRCC	3 cut(s)	42, 409, 1411
AccB7I	CCANNNNNTGG	1 cut(s)	52
AccI	GTMKAC	1 cut(s)	132
AccII	CGCG	3 cut(s)	273, 612, 1114
AciI	CCGC	9 cut(s)	30, 33, 36, 39, 247, 529, 570, 765, 1112
AclI	AACGTT	2 cut(s)	1074, 1320
AclWI	GGATC	4 cut(s)	142, 187, 1392, 1612
AcoI	YGGCCR	1 cut(s)	429
AcsI	RAATTY	1 cut(s)	1029
AcuI	CTGAAG	1 cut(s)	1103
AcyI	GRCGYC	1 cut(s)	1412
AfaI	GTAC	3 cut(s)	65, 155, 1620
AfiI	CCNNNNNNNGG	3 cut(s)	29, 52, 326
AflIII	ACRYGT	1 cut(s)	135
AgsI	TTSAA	9 cut(s)	365, 440, 491, 955, 1037, 1107, 1325, 1453, 1533
AjiI	CACGTC	1 cut(s)	136
AjnI	CCWGG	1 cut(s)	229
AjuI	GAANNNNNNNTTGG	2 cut(s)	1445, 1477
AloI	GAACNNNNNNTCC	2 cut(s)	1305, 1337
AluBI	AGCT	7 cut(s)	109, 580, 682, 909, 1121, 1270, 1709
AluI	AGCT	7 cut(s)	109, 580, 682, 909, 1121, 1270, 1709
Alw21I	GWGCWC	1 cut(s)	172
Alw26I	GTCTC	1 cut(s)	544
AlwI	GGATC	4 cut(s)	142, 187, 1392, 1612
AlwNI	CAGNNNCTG	2 cut(s)	322, 731
Ama87I	CYCGRG	1 cut(s)	1404
AoxI	GGCC	1 cut(s)	429
ApeKI	GCWGC	6 cut(s)	119, 316, 343, 725, 1160, 1492
ApoI	RAATTY	1 cut(s)	1029
AspLEI	GCGC	4 cut(s)	79, 273, 612, 1414
AspS9I	GGNCC	1 cut(s)	869
AsuHPI	GGTGA	2 cut(s)	609, 1495
AvaI	CYCGRG	1 cut(s)	1404
AvaII	GGWCC	1 cut(s)	869
BanI	GGYRCC	3 cut(s)	42, 409, 1411
BbsI	GAAGAC	3 cut(s)	76, 1234, 1473
Bbv12I	GWGCWC	1 cut(s)	172
BbvI	GCAGC	6 cut(s)	106, 328, 330, 737, 1147, 1504
BccI	CCATC	5 cut(s)	395, 632, 636, 659, 1217
BceAI	ACGGC	1 cut(s)	1160
BciT130I	CCWGG	1 cut(s)	231
BciVI	GTATCC	1 cut(s)	566
BclI	TGATCA	1 cut(s)	913
BcoDI	GTCTC	1 cut(s)	544
BfaI	CTAG	6 cut(s)	792, 1047, 1271, 1536, 1566, 1647
BfmI	CTRYAG	1 cut(s)	696
BfoI	RGCGCY	1 cut(s)	1415
BfuI	GTATCC	1 cut(s)	566
BisI	GCNGC	9 cut(s)	31, 34, 37, 120, 317, 344, 726, 1161, 1493
BlsI	GCNGC	9 cut(s)	32, 35, 38, 121, 318, 345, 727, 1162, 1494
Bme1390I	CCNGG	1 cut(s)	231
Bme18I	GGWCC	1 cut(s)	869
BmeT110I	CYCGRG	1 cut(s)	1404
BmgBI	CACGTC	1 cut(s)	136
BmgT120I	GGNCC	1 cut(s)	869
BmiI	GGNNCC	3 cut(s)	44, 411, 1413
BmrFI	CCNGG	1 cut(s)	231
BmsI	GCATC	2 cut(s)	661, 713
BpiI	GAAGAC	3 cut(s)	76, 1234, 1473
BpmI	CTGGAG	2 cut(s)	74, 990
BpuEI	CTTGAG	1 cut(s)	803
BsaAI	YACGTR	1 cut(s)	1694
BsaBI	GATNNNNATC	1 cut(s)	675
BsaHI	GRCGYC	1 cut(s)	1412
BsaJI	CCNNGG	1 cut(s)	46
BsaWI	WCCGGW	1 cut(s)	164
BsaXI	ACNNNNNCTCC	4 cut(s)	47, 77, 146, 176
Bsc4I	CCNNNNNNNGG	3 cut(s)	29, 52, 326
Bse118I	RCCGGY	1 cut(s)	26
Bse1I	ACTGG	3 cut(s)	151, 571, 1213
Bse3DI	GCAATG	1 cut(s)	763
Bse8I	GATNNNNATC	1 cut(s)	675
BseBI	CCWGG	1 cut(s)	231
BseDI	CCNNGG	1 cut(s)	46
BseGI	GGATG	4 cut(s)	87, 261, 647, 1518
BseJI	GATNNNNATC	1 cut(s)	675
BseLI	CCNNNNNNNGG	3 cut(s)	29, 52, 326
BseMI	GCAATG	1 cut(s)	763
BseMII	CTCAG	2 cut(s)	1055, 1269
BseNI	ACTGG	3 cut(s)	151, 571, 1213
BseXI	GCAGC	6 cut(s)	106, 328, 330, 737, 1147, 1504
BsgI	GTGCAG	1 cut(s)	1511
Bsh1236I	CGCG	3 cut(s)	273, 612, 1114
BshFI	GGCC	1 cut(s)	431
BshNI	GGYRCC	3 cut(s)	42, 409, 1411
BsiHKAI	GWGCWC	1 cut(s)	172
BsiHKCI	CYCGRG	1 cut(s)	1404
BsiSI	CCGG	2 cut(s)	27, 165
BslFI	GGGAC	1 cut(s)	986
BslI	CCNNNNNNNGG	3 cut(s)	29, 52, 326
BsmAI	GTCTC	1 cut(s)	544
BsmFI	GGGAC	1 cut(s)	986
BsnI	GGCC	1 cut(s)	431
BsoBI	CYCGRG	1 cut(s)	1404
Bsp1286I	GDGCHC	1 cut(s)	172
Bsp143I	GATC	7 cut(s)	147, 179, 238, 913, 1384, 1604, 1660
BspACI	CCGC	9 cut(s)	30, 33, 36, 39, 247, 529, 570, 765, 1112
BspANI	GGCC	1 cut(s)	431
BspCNI	CTCAG	2 cut(s)	1056, 1270
BspFNI	CGCG	3 cut(s)	273, 612, 1114
BspHI	TCATGA	1 cut(s)	391
BspLI	GGNNCC	3 cut(s)	44, 411, 1413
BspPI	GGATC	4 cut(s)	142, 187, 1392, 1612
BspT107I	GGYRCC	3 cut(s)	42, 409, 1411
BsrDI	GCAATG	1 cut(s)	763
BsrFI	RCCGGY	1 cut(s)	26
BsrI	ACTGG	3 cut(s)	151, 571, 1213
BssAI	RCCGGY	1 cut(s)	26
BssECI	CCNNGG	1 cut(s)	46
BssMI	GATC	7 cut(s)	147, 179, 238, 913, 1384, 1604, 1660
BssNI	GRCGYC	1 cut(s)	1412
BssT1I	CCWWGG	1 cut(s)	46
Bst2UI	CCWGG	1 cut(s)	231
Bst4CI	ACNGT	5 cut(s)	127, 253, 1137, 1343, 1392
BstACI	GRCGYC	1 cut(s)	1412
BstAPI	GCANNNNNTGC	2 cut(s)	731, 1501
BstBAI	YACGTR	1 cut(s)	1694
BstC8I	GCNNGC	7 cut(s)	24, 28, 100, 189, 211, 552, 923
BstDEI	CTNAG	4 cut(s)	822, 999, 1064, 1278
BstENI	CCTNNNNNAGG	1 cut(s)	324
BstF5I	GGATG	4 cut(s)	87, 261, 647, 1518
BstFNI	CGCG	3 cut(s)	273, 612, 1114
BstH2I	RGCGCY	1 cut(s)	1415
BstHHI	GCGC	4 cut(s)	79, 273, 612, 1414
BstKTI	GATC	7 cut(s)	150, 182, 241, 916, 1387, 1607, 1663
BstMAI	GTCTC	1 cut(s)	544
BstMBI	GATC	7 cut(s)	147, 179, 238, 913, 1384, 1604, 1660
BstMWI	GCNNNNNNNGC	9 cut(s)	23, 36, 42, 340, 515, 701, 731, 762, 1501
BstNI	CCWGG	1 cut(s)	231
BstSCI	CCNGG	1 cut(s)	229
BstSFI	CTRYAG	1 cut(s)	696
BstUI	CGCG	3 cut(s)	273, 612, 1114
BstV1I	GCAGC	6 cut(s)	106, 328, 330, 737, 1147, 1504
BstV2I	GAAGAC	3 cut(s)	76, 1234, 1473
BstX2I	RGATCY	2 cut(s)	179, 1384
BstXI	CCANNNNNNTGG	1 cut(s)	209
BstYI	RGATCY	2 cut(s)	179, 1384
BsuI	GTATCC	1 cut(s)	566
BsuRI	GGCC	1 cut(s)	431
BtrI	CACGTC	1 cut(s)	136
BtsCI	GGATG	4 cut(s)	87, 261, 647, 1518
BtsIMutI	CAGTG	4 cut(s)	249, 294, 747, 1206
Cac8I	GCNNGC	7 cut(s)	24, 28, 100, 189, 211, 552, 923
CaiI	CAGNNNCTG	2 cut(s)	322, 731
CciI	TCATGA	1 cut(s)	391
CfoI	GCGC	4 cut(s)	79, 273, 612, 1414
Cfr10I	RCCGGY	1 cut(s)	26
Cfr13I	GGNCC	1 cut(s)	869
CseI	GACGC	1 cut(s)	125
Csp6I	GTAC	3 cut(s)	64, 154, 1619
CviAII	CATG	7 cut(s)	61, 206, 392, 969, 992, 1130, 1496
CviQI	GTAC	3 cut(s)	64, 154, 1619
DdeI	CTNAG	4 cut(s)	822, 999, 1064, 1278
DinI	GGCGCC	1 cut(s)	1413
DpnI	GATC	7 cut(s)	149, 181, 240, 915, 1386, 1606, 1662
DpnII	GATC	7 cut(s)	147, 179, 238, 913, 1384, 1604, 1660
EaeI	YGGCCR	1 cut(s)	429
EciI	GGCGGA	1 cut(s)	585
Eco130I	CCWWGG	1 cut(s)	46
Eco47I	GGWCC	1 cut(s)	869
Eco57I	CTGAAG	1 cut(s)	1103
Eco88I	CYCGRG	1 cut(s)	1404
EcoNI	CCTNNNNNAGG	1 cut(s)	324
EcoO109I	RGGNCCY	1 cut(s)	869
EcoRII	CCWGG	1 cut(s)	229
EcoT14I	CCWWGG	1 cut(s)	46
EgeI	GGCGCC	1 cut(s)	1413
EheI	GGCGCC	1 cut(s)	1413
ErhI	CCWWGG	1 cut(s)	46
FaeI	CATG	7 cut(s)	64, 209, 395, 972, 995, 1133, 1499
FaqI	GGGAC	1 cut(s)	986
FatI	CATG	7 cut(s)	60, 205, 391, 968, 991, 1129, 1495
FauI	CCCGC	2 cut(s)	32, 254
FauNDI	CATATG	1 cut(s)	709
FbaI	TGATCA	1 cut(s)	913
FblI	GTMKAC	1 cut(s)	132
Fnu4HI	GCNGC	9 cut(s)	31, 34, 37, 120, 317, 344, 726, 1161, 1493
FokI	GGATG	4 cut(s)	74, 248, 654, 1525
Fsp4HI	GCNGC	9 cut(s)	31, 34, 37, 120, 317, 344, 726, 1161, 1493
FspBI	CTAG	6 cut(s)	792, 1047, 1271, 1536, 1566, 1647
GlaI	GCGC	4 cut(s)	78, 272, 611, 1413
GluI	GCNGC	9 cut(s)	31, 34, 37, 120, 317, 344, 726, 1161, 1493
GsuI	CTGGAG	2 cut(s)	74, 990
HaeII	RGCGCY	1 cut(s)	1415
HaeIII	GGCC	1 cut(s)	431
HapII	CCGG	2 cut(s)	27, 165
HgaI	GACGC	1 cut(s)	125
HhaI	GCGC	4 cut(s)	79, 273, 612, 1414
Hin1I	GRCGYC	1 cut(s)	1412
Hin1II	CATG	7 cut(s)	64, 209, 395, 972, 995, 1133, 1499
Hin6I	GCGC	4 cut(s)	77, 271, 610, 1412
HinP1I	GCGC	4 cut(s)	77, 271, 610, 1412
HincII	GTYRAC	2 cut(s)	133, 858
HindII	GTYRAC	2 cut(s)	133, 858
HindIII	AAGCTT	1 cut(s)	578
HinfI	GANTC	4 cut(s)	56, 988, 1176, 1529
HpaI	GTTAAC	1 cut(s)	858
HpaII	CCGG	2 cut(s)	27, 165
HphI	GGTGA	2 cut(s)	609, 1495
Hpy166II	GTNNAC	4 cut(s)	133, 858, 1486, 1518
Hpy188I	TCNGA	5 cut(s)	179, 238, 906, 1083, 1259
Hpy8I	GTNNAC	4 cut(s)	133, 858, 1486, 1518
Hpy99I	CGWCG	6 cut(s)	98, 119, 131, 134, 137, 1683
HpyAV	CCTTC	6 cut(s)	298, 828, 1031, 1304, 1371, 1450
HpyCH4III	ACNGT	5 cut(s)	127, 253, 1137, 1343, 1392
HpyCH4IV	ACGT	6 cut(s)	135, 1074, 1320, 1488, 1551, 1693
HpyCH4V	TGCA	5 cut(s)	220, 674, 1250, 1334, 1492
HpyF10VI	GCNNNNNNNGC	9 cut(s)	23, 36, 42, 340, 515, 701, 731, 762, 1501
HpyF3I	CTNAG	4 cut(s)	822, 999, 1064, 1278
HpySE526I	ACGT	6 cut(s)	135, 1074, 1320, 1488, 1551, 1693
Hsp92I	GRCGYC	1 cut(s)	1412
Hsp92II	CATG	7 cut(s)	64, 209, 395, 972, 995, 1133, 1499
HspAI	GCGC	4 cut(s)	77, 271, 610, 1412
KasI	GGCGCC	1 cut(s)	1411
KroI	GCCGGC	1 cut(s)	26
KroNI	GCCGGC	1 cut(s)	28
Ksp22I	TGATCA	1 cut(s)	913
KspAI	GTTAAC	1 cut(s)	858
Kzo9I	GATC	7 cut(s)	147, 179, 238, 913, 1384, 1604, 1660
LmnI	GCTCC	5 cut(s)	175, 232, 906, 1126, 1192
Lsp1109I	GCAGC	6 cut(s)	106, 328, 330, 737, 1147, 1504
LweI	GCATC	2 cut(s)	661, 713
MaeI	CTAG	6 cut(s)	792, 1047, 1271, 1536, 1566, 1647
MaeII	ACGT	6 cut(s)	135, 1074, 1320, 1488, 1551, 1693
MaeIII	GTNAC	5 cut(s)	292, 1153, 1304, 1392, 1570
MalI	GATC	7 cut(s)	149, 181, 240, 915, 1386, 1606, 1662
MboI	GATC	7 cut(s)	147, 179, 238, 913, 1384, 1604, 1660
MboII	GAAGA	8 cut(s)	76, 430, 503, 1070, 1105, 1239, 1465, 1478
MflI	RGATCY	2 cut(s)	179, 1384
MhlI	GDGCHC	1 cut(s)	172
MluCI	AATT	5 cut(s)	421, 443, 841, 1029, 1460
Mly113I	GGCGCC	1 cut(s)	1412
MlyI	GAGTC	2 cut(s)	65, 1170
MmeI	TCCRAC	2 cut(s)	710, 1362
MroNI	GCCGGC	1 cut(s)	26
MseI	TTAA	4 cut(s)	857, 1071, 1356, 1548
MslI	CAYNNNNRTG	1 cut(s)	1299
MspI	CCGG	2 cut(s)	27, 165
MspR9I	CCNGG	1 cut(s)	231
MvaI	CCWGG	1 cut(s)	231
MvnI	CGCG	3 cut(s)	273, 612, 1114
MwoI	GCNNNNNNNGC	9 cut(s)	23, 36, 42, 340, 515, 701, 731, 762, 1501
NaeI	GCCGGC	1 cut(s)	28
NarI	GGCGCC	1 cut(s)	1412
NdeI	CATATG	1 cut(s)	709
NdeII	GATC	7 cut(s)	147, 179, 238, 913, 1384, 1604, 1660
NgoMIV	GCCGGC	1 cut(s)	26
NlaIII	CATG	7 cut(s)	64, 209, 395, 972, 995, 1133, 1499
NlaIV	GGNNCC	3 cut(s)	44, 411, 1413
NmeAIII	GCCGAG	1 cut(s)	163
NmuCI	GTSAC	4 cut(s)	292, 1304, 1392, 1570
PaeR7I	CTCGAG	1 cut(s)	1404
PagI	TCATGA	1 cut(s)	391
PcsI	WCGNNNNNNNCGW	2 cut(s)	99, 123
PdiI	GCCGGC	1 cut(s)	28
PfeI	GAWTC	2 cut(s)	988, 1529
PflMI	CCANNNNNTGG	1 cut(s)	52
PkrI	GCNGC	9 cut(s)	32, 35, 38, 121, 318, 345, 727, 1162, 1494
PleI	GAGTC	2 cut(s)	64, 1170
PluTI	GGCGCC	1 cut(s)	1415
PpsI	GAGTC	2 cut(s)	64, 1170
Ppu21I	YACGTR	1 cut(s)	1694
PpuMI	RGGWCCY	1 cut(s)	869
PsiI	TTATAA	1 cut(s)	876
Psp1406I	AACGTT	2 cut(s)	1074, 1320
Psp5II	RGGWCCY	1 cut(s)	869
Psp6I	CCWGG	1 cut(s)	229
PspGI	CCWGG	1 cut(s)	229
PspN4I	GGNNCC	3 cut(s)	44, 411, 1413
PspPI	GGNCC	1 cut(s)	869
PspPPI	RGGWCCY	1 cut(s)	869
PspXI	VCTCGAGB	1 cut(s)	1404
PstNI	CAGNNNCTG	2 cut(s)	322, 731
PsuI	RGATCY	2 cut(s)	179, 1384
RsaI	GTAC	3 cut(s)	65, 155, 1620
RsaNI	GTAC	3 cut(s)	64, 154, 1619
RseI	CAYNNNNRTG	1 cut(s)	1299
SalI	GTCGAC	1 cut(s)	131
SaqAI	TTAA	4 cut(s)	857, 1071, 1356, 1548
SatI	GCNGC	9 cut(s)	31, 34, 37, 120, 317, 344, 726, 1161, 1493
Sau3AI	GATC	7 cut(s)	147, 179, 238, 913, 1384, 1604, 1660
Sau96I	GGNCC	1 cut(s)	869
SchI	GAGTC	2 cut(s)	65, 1170
ScrFI	CCNGG	1 cut(s)	231
SduI	GDGCHC	1 cut(s)	172
SfaNI	GCATC	2 cut(s)	661, 713
SfcI	CTRYAG	1 cut(s)	696
SfoI	GGCGCC	1 cut(s)	1413
Sfr274I	CTCGAG	1 cut(s)	1404
SgrDI	CGTCGACG	1 cut(s)	131
SinI	GGWCC	1 cut(s)	869
SlaI	CTCGAG	1 cut(s)	1404
SmiMI	CAYNNNNRTG	1 cut(s)	1299
SmlI	CTYRAG	3 cut(s)	12, 782, 1404
SmoI	CTYRAG	3 cut(s)	12, 782, 1404
Sse9I	AATT	5 cut(s)	421, 443, 841, 1029, 1460
SsiI	CCGC	9 cut(s)	30, 33, 36, 39, 247, 529, 570, 765, 1112
SspDI	GGCGCC	1 cut(s)	1411
SspI	AATATT	1 cut(s)	1102
SspMI	CTAG	6 cut(s)	792, 1047, 1271, 1536, 1566, 1647
StyD4I	CCNGG	1 cut(s)	229
StyI	CCWWGG	1 cut(s)	46
TaaI	ACNGT	5 cut(s)	127, 253, 1137, 1343, 1392
TaiI	ACGT	6 cut(s)	138, 1077, 1323, 1491, 1554, 1696
TaqI	TCGA	5 cut(s)	68, 132, 1405, 1578, 1678
TasI	AATT	5 cut(s)	421, 443, 841, 1029, 1460
TatI	WGTACW	2 cut(s)	63, 1618
TauI	GCSGC	3 cut(s)	33, 36, 39
TfiI	GAWTC	2 cut(s)	988, 1529
Tru1I	TTAA	4 cut(s)	857, 1071, 1356, 1548
Tru9I	TTAA	4 cut(s)	857, 1071, 1356, 1548
TscAI	CASTG	4 cut(s)	256, 301, 747, 1213
TseFI	GTSAC	4 cut(s)	292, 1304, 1392, 1570
TseI	GCWGC	6 cut(s)	119, 316, 343, 725, 1160, 1492
Tsp45I	GTSAC	4 cut(s)	292, 1304, 1392, 1570
TspDTI	ATGAA	7 cut(s)	76, 408, 431, 443, 980, 1146, 1257
TspRI	CASTG	4 cut(s)	256, 301, 747, 1213
Van91I	CCANNNNNTGG	1 cut(s)	52
VpaK11BI	GGWCC	1 cut(s)	869
XagI	CCTNNNNNAGG	1 cut(s)	324
XapI	RAATTY	1 cut(s)	1029
XhoI	CTCGAG	1 cut(s)	1404
XmiI	GTMKAC	1 cut(s)	132
XspI	CTAG	6 cut(s)	792, 1047, 1271, 1536, 1566, 1647

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.