Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0008595

Physical Location & Seq

Genomic Coordinates Forward (+)

43775192 .. 43785948

Gene Structure 10757 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G028810.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1758 bp

ATGGGCCACGGGAAGCCTGGCCAGCGCCAAGTGCTGGAGTCCGTCTACTCCGTGATCGCGCTCTTCTTCATTCTCGTCGCCTGCGTCGAGCTCTGCGACGCCGCGACTGTCGTCGACGTCTACCGCCTGATCCAATACGACATGTCGGGAGCTCAGTTCGGATCTCGCCTCGCCAATCTGAATCACCACGCCGGCTCCTTGCACTCGCCGCCTGGCTCCGATCTCTCTCGCACCGTCCTCATCATCCCCTTGCGTGAACTCAACATCACCTTCGTCAGAGAATATATTATGCAGAAGCAGCCTTTGGGAGGCTTGCTGATTTTGCTTCCCCAGATGTTTAGTCCCGAAAATAGAGAAACGGCAAAAAGTAGTTGCGAGACTGATGGAGAGGATGTTTTGAAGAATGTTTTGGCTGAACTTGAAAAGCTACTTATACATTCCAAAATACCTTATCCTGTATATTTTGCTTTTGAAGATGATGATATTGAGGCTGTGTTGGCTGATGTCAAGAGGAATGATGCCACCGGGCAGCCGGCTACTGCGACCACTGGAGGATACAAGCTTGTTGTTTCAGTTCCAGAACCTAAGAAACGTGCATCACCCACCATTACAAACATTCAGGGATGGTTGTCAGGGTTGAAAACTGATGGAGACACAAATCAACTACCAACTATCGCTATTGTAGCATCATATGACACATTTGGGGCAGCTCCTTCTTTATCAGTGGGAAGTGATAGCAATGGAAGTGGGGTTGTCGCCCTACTTGAAATTGCTAGGTTGTTTTCTGTTCTATACTCAAATCCCAAGACGAGAGGAAGGTACAATTTGCTTTTTGGGTTGACATCTGGAGGACCTTATAACTACAATGGAACTAATAAGTGGCTCCGGAGCTTTGACCAACGCCTGCGTGAAAGTGTTGACTACGCTATTTGCTTGAATAGTATTGGCTCATGGGACAAAGAATTGTGGGTTCATGTCTCTAAGCCTCCAGAGAATGCCTACATAAAGCAAATATTTGAAGGTTTCTCTGATGTAGCAGAAGAGTTGGGCATTACGGTTGGTCTGAAGCACAAGAAAATAAATATTTCTAATCCTCGAGTAGCTTGGGAACATGAACAGTTTTCACGGCTGAGAGTTACTGCTGCCACCCTTTCCGAACTCTCTGTTGCTCCTGAACTGTTAGAAAGCACTGGAAGTCTGCTTGATGGCAGGCAATTTGTTAATGAAACTGCAATCATTAAAAGTGTCAAGTTAGTTGCTGAGAGCCTTGCGAGGCATATCTATGGTCACAAAGGAAAGAACATTCAAATCTTTGCAGAAAACAGCAGTTTGGCAGTTAATCCTTCATACATAAAATCTTGGTTGGATCTATTGTCGCAAACACCTCGAGTGGCGCCATTTTTATCAAAGAATGATCCATTTATCTTGGCATTGAAAAAGTTGATTGCAAGGCCTTACTGTGTGAGATTCATAAGTTTTTTTGGAGGAAGGAAGATGGGAATGGAGTTCGAGGATGTGCAGTGTTGGGGCAATCTTTTGGATGGCCGGGAGAGAACAGAACAGAAAGATCTTTCAGGAGCTAGGGGTGAGGAGGTAGATCACTTGTGGGATGGTGATCAATATTTTGTGTCCCTTTGGGGATCAGCTTCTTCAGCATTTACAGATAGCTCGTTTCATGCTATATATATAATTGAATTGGAATTTGGTAATCTGTTGTACTTTAGCTTTTTGTGCTATTTGGTTCTTAGTTTTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006355 GO:0006357 GO:0007275 GO:0007399 GO:0008150 GO:0009889 GO:0009966 GO:0009968 GO:0010468 GO:0010556 GO:0010646 GO:0010648 GO:0012505 GO:0016020 GO:0019219 GO:0019222 GO:0023051 GO:0023057 GO:0031323 GO:0031326 GO:0031984 GO:0032501 GO:0032502 GO:0032925 GO:0032926 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048483 GO:0048484 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051171 GO:0051239 GO:0051241 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0090092 GO:0090101 GO:0098827 GO:1900107 GO:1900108 GO:1900145 GO:1900146 GO:1900175 GO:1900176 GO:1903506 GO:2000026 GO:2000112 GO:2000380 GO:2000381 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Nicastrin

Protein Analysis

585

Amino Acids

64.92

Weight (kDa)

5.74

Isoelectric Point (pI)

37.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nicastrin	PF05450	224 - 328	6.2e-06	Nicastrin large lobe

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008595)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44330
fragaria_vesca	FvH4_3g25670 FvH4_3g25670 FvH4_3g25670 FvH4_3g25670
malus_domestica	MD03G1149800.v1.1 MD11G1168600.v1.1
prunus_persica	Prupe.6G135800_v2.0.a1
pyrus_communis	pycom11g14130
rosa_chinensis	RchiOBHm_Chr5g0045881
rosa_laevigata	RLG00000034394
rosa_multiflora	Rmu_sc0001212.1_g000004
rosa_roxburghii	Rroxscaffold_1G00028740 Rroxscaffold_1G00035230 Rroxscaffold_2G00117520 Rroxscaffold_2G00117530
rosa_rugosa	Rorug05G0226200 Rorug05G0226300
rosa_samantha	Rh5AG307700 Rh5BG316000 Rh5CG342200 Rh5DG326300
rosa_wichuraiana	Rw5G028810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	858
AatII	GACGTC	1 cut(s)	120
AccB1I	GGYRCC	1 cut(s)	1393
AccB7I	CCANNNNNTGG	1 cut(s)	34
AccI	GTMKAC	3 cut(s)	45, 114, 120
AccII	CGCG	2 cut(s)	59, 104
AccIII	TCCGGA	1 cut(s)	885
AciI	CCGC	3 cut(s)	102, 124, 209
AclWI	GGATC	5 cut(s)	124, 169, 1374, 1409, 1648
AcoI	YGGCCR	2 cut(s)	19, 1543
AcsI	RAATTY	1 cut(s)	1700
AcuI	CTGAAG	2 cut(s)	1085, 1635
AcyI	GRCGYC	3 cut(s)	99, 117, 1394
AfaI	GTAC	2 cut(s)	821, 1718
AfiI	CCNNNNNNNGG	2 cut(s)	34, 308
AflIII	ACRYGT	1 cut(s)	141
AjnI	CCWGG	2 cut(s)	16, 211
AjuI	GAANNNNNNNTTGG	6 cut(s)	287, 319, 392, 424, 1686, 1718
Alw21I	GWGCWC	2 cut(s)	93, 154
Alw26I	GTCTC	3 cut(s)	371, 645, 982
AlwI	GGATC	5 cut(s)	124, 169, 1374, 1409, 1648
Ama87I	CYCGRG	2 cut(s)	1095, 1386
Aor13HI	TCCGGA	1 cut(s)	885
AoxI	GGCC	4 cut(s)	4, 19, 1451, 1543
ApeKI	GCWGC	4 cut(s)	298, 529, 707, 1142
ApoI	RAATTY	1 cut(s)	1700
AspLEI	GCGC	3 cut(s)	27, 61, 1396
AspS9I	GGNCC	2 cut(s)	4, 851
AsuC2I	CCSGG	2 cut(s)	526, 1547
AsuHPI	GGTGA	5 cut(s)	176, 259, 591, 1598, 1625
AvaI	CYCGRG	2 cut(s)	1095, 1386
AvaII	GGWCC	1 cut(s)	851
BalI	TGGCCA	1 cut(s)	21
BanI	GGYRCC	1 cut(s)	1393
BanII	GRGCYC	2 cut(s)	93, 154
Bbv12I	GWGCWC	2 cut(s)	93, 154
BbvI	GCAGC	4 cut(s)	310, 541, 719, 1129
BccI	CCATC	7 cut(s)	377, 618, 641, 1199, 1489, 1535, 1604
BceAI	ACGGC	2 cut(s)	375, 1142
BcgI	CGANNNNNNTGC	2 cut(s)	1367, 1401
BciT130I	CCWGG	2 cut(s)	18, 213
BciVI	GTATCC	1 cut(s)	548
BclI	TGATCA	1 cut(s)	1615
BcnI	CCSGG	2 cut(s)	526, 1547
BcoDI	GTCTC	3 cut(s)	371, 645, 982
BfaI	CTAG	2 cut(s)	774, 1581
BfoI	RGCGCY	2 cut(s)	28, 1397
BfuI	GTATCC	1 cut(s)	548
BglII	AGATCT	1 cut(s)	1567
BisI	GCNGC	6 cut(s)	102, 209, 299, 530, 708, 1143
BlsI	GCNGC	6 cut(s)	103, 210, 300, 531, 709, 1144
Bme1390I	CCNGG	4 cut(s)	18, 213, 526, 1547
Bme18I	GGWCC	1 cut(s)	851
BmeT110I	CYCGRG	2 cut(s)	1095, 1386
BmgT120I	GGNCC	2 cut(s)	4, 851
BmiI	GGNNCC	4 cut(s)	196, 217, 884, 1395
BmrFI	CCNGG	4 cut(s)	18, 213, 526, 1547
BmsI	GCATC	3 cut(s)	508, 605, 695
BoxI	GACNNNNGTC	1 cut(s)	110
BpmI	CTGGAG	4 cut(s)	56, 570, 867, 972
BpuMI	CCSGG	2 cut(s)	526, 1547
BsaBI	GATNNNNATC	1 cut(s)	1614
BsaHI	GRCGYC	3 cut(s)	99, 117, 1394
BsaJI	CCNNGG	1 cut(s)	7
BsaWI	WCCGGW	1 cut(s)	885
BsaXI	ACNNNNNCTCC	4 cut(s)	179, 209, 378, 408
Bsc4I	CCNNNNNNNGG	2 cut(s)	34, 308
Bse118I	RCCGGY	2 cut(s)	191, 532
Bse1I	ACTGG	2 cut(s)	553, 1195
Bse3DI	GCAATG	1 cut(s)	745
Bse8I	GATNNNNATC	1 cut(s)	1614
BseAI	TCCGGA	1 cut(s)	885
BseBI	CCWGG	2 cut(s)	18, 213
BseDI	CCNNGG	1 cut(s)	7
BseGI	GGATG	6 cut(s)	243, 397, 629, 1519, 1546, 1615
BseJI	GATNNNNATC	1 cut(s)	1614
BseLI	CCNNNNNNNGG	2 cut(s)	34, 308
BseMI	GCAATG	1 cut(s)	745
BseMII	CTCAG	3 cut(s)	167, 1121, 1251
BseNI	ACTGG	2 cut(s)	553, 1195
BseRI	GAGGAG	1 cut(s)	1604
BseXI	GCAGC	4 cut(s)	310, 541, 719, 1129
BsgI	GTGCAG	1 cut(s)	1538
Bsh1236I	CGCG	2 cut(s)	59, 104
BshFI	GGCC	4 cut(s)	6, 21, 1453, 1545
BshNI	GGYRCC	1 cut(s)	1393
BsiHKAI	GWGCWC	2 cut(s)	93, 154
BsiHKCI	CYCGRG	2 cut(s)	1095, 1386
BsiSI	CCGG	5 cut(s)	192, 525, 533, 886, 1546
BslFI	GGGAC	3 cut(s)	327, 968, 1615
BslI	CCNNNNNNNGG	2 cut(s)	34, 308
BsmAI	GTCTC	3 cut(s)	371, 645, 982
BsmFI	GGGAC	3 cut(s)	327, 968, 1615
BsmI	GAATGC	1 cut(s)	1000
BsnI	GGCC	4 cut(s)	6, 21, 1453, 1545
BsoBI	CYCGRG	2 cut(s)	1095, 1386
Bsp1286I	GDGCHC	2 cut(s)	93, 154
Bsp13I	TCCGGA	1 cut(s)	885
BspACI	CCGC	3 cut(s)	102, 124, 209
BspANI	GGCC	4 cut(s)	6, 21, 1453, 1545
BspCNI	CTCAG	3 cut(s)	166, 1122, 1252
BspEI	TCCGGA	1 cut(s)	885
BspFNI	CGCG	2 cut(s)	59, 104
BspLI	GGNNCC	4 cut(s)	196, 217, 884, 1395
BspPI	GGATC	5 cut(s)	124, 169, 1374, 1409, 1648
BspQI	GCTCTTC	1 cut(s)	68
BspT107I	GGYRCC	1 cut(s)	1393
BsrDI	GCAATG	1 cut(s)	745
BsrFI	RCCGGY	2 cut(s)	191, 532
BsrI	ACTGG	2 cut(s)	553, 1195
BssAI	RCCGGY	2 cut(s)	191, 532
BssECI	CCNNGG	1 cut(s)	7
BssNI	GRCGYC	3 cut(s)	99, 117, 1394
Bst2UI	CCWGG	2 cut(s)	18, 213
Bst4CI	ACNGT	6 cut(s)	109, 235, 1057, 1119, 1179, 1460
Bst6I	CTCTTC	2 cut(s)	68, 1035
BstACI	GRCGYC	3 cut(s)	99, 117, 1394
BstC8I	GCNNGC	7 cut(s)	23, 82, 193, 314, 534, 905, 1211
BstDEI	CTNAG	6 cut(s)	153, 585, 981, 1130, 1260, 1745
BstDSI	CCRYGG	1 cut(s)	7
BstENI	CCTNNNNNAGG	1 cut(s)	306
BstF5I	GGATG	6 cut(s)	243, 397, 629, 1519, 1546, 1615
BstFNI	CGCG	2 cut(s)	59, 104
BstH2I	RGCGCY	2 cut(s)	28, 1397
BstHHI	GCGC	3 cut(s)	27, 61, 1396
BstMAI	GTCTC	3 cut(s)	371, 645, 982
BstMWI	GCNNNNNNNGC	9 cut(s)	22, 31, 208, 298, 322, 497, 683, 1652, 1731
BstNI	CCWGG	2 cut(s)	18, 213
BstNSI	RCATGY	1 cut(s)	145
BstPAI	GACNNNNGTC	1 cut(s)	110
BstSCI	CCNGG	4 cut(s)	16, 211, 524, 1545
BstUI	CGCG	2 cut(s)	59, 104
BstV1I	GCAGC	4 cut(s)	310, 541, 719, 1129
BstX2I	RGATCY	3 cut(s)	161, 1366, 1567
BstYI	RGATCY	3 cut(s)	161, 1366, 1567
BsuI	GTATCC	1 cut(s)	548
BsuRI	GGCC	4 cut(s)	6, 21, 1453, 1545
BtgI	CCRYGG	1 cut(s)	7
BtsCI	GGATG	6 cut(s)	243, 397, 629, 1519, 1546, 1615
BtsI	GCAGTG	1 cut(s)	1526
BtsIMutI	CAGTG	4 cut(s)	546, 729, 1188, 1526
Cac8I	GCNNGC	7 cut(s)	23, 82, 193, 314, 534, 905, 1211
CfoI	GCGC	3 cut(s)	27, 61, 1396
Cfr10I	RCCGGY	2 cut(s)	191, 532
Cfr13I	GGNCC	2 cut(s)	4, 851
CseI	GACGC	2 cut(s)	73, 107
Csp6I	GTAC	2 cut(s)	820, 1717
CspCI	CAANNNNNGTGG	2 cut(s)	727, 762
CviAII	CATG	5 cut(s)	142, 951, 974, 1112, 1676
CviQI	GTAC	2 cut(s)	820, 1717
DdeI	CTNAG	6 cut(s)	153, 585, 981, 1130, 1260, 1745
DinI	GGCGCC	1 cut(s)	1395
EaeI	YGGCCR	2 cut(s)	19, 1543
Eam1104I	CTCTTC	2 cut(s)	68, 1035
EarI	CTCTTC	2 cut(s)	68, 1035
Ecl136II	GAGCTC	2 cut(s)	91, 152
Eco147I	AGGCCT	1 cut(s)	1453
Eco24I	GRGCYC	2 cut(s)	93, 154
Eco47I	GGWCC	1 cut(s)	851
Eco53kI	GAGCTC	2 cut(s)	91, 152
Eco57I	CTGAAG	2 cut(s)	1085, 1635
Eco88I	CYCGRG	2 cut(s)	1095, 1386
EcoICRI	GAGCTC	2 cut(s)	91, 152
EcoNI	CCTNNNNNAGG	1 cut(s)	306
EcoO109I	RGGNCCY	1 cut(s)	851
EcoRII	CCWGG	2 cut(s)	16, 211
EcoT38I	GRGCYC	2 cut(s)	93, 154
EgeI	GGCGCC	1 cut(s)	1395
EheI	GGCGCC	1 cut(s)	1395
FaeI	CATG	5 cut(s)	145, 954, 977, 1115, 1679
FaqI	GGGAC	3 cut(s)	327, 968, 1615
FatI	CATG	5 cut(s)	141, 950, 973, 1111, 1675
FauNDI	CATATG	1 cut(s)	691
FbaI	TGATCA	1 cut(s)	1615
FblI	GTMKAC	3 cut(s)	45, 114, 120
Fnu4HI	GCNGC	6 cut(s)	102, 209, 299, 530, 708, 1143
FokI	GGATG	6 cut(s)	230, 404, 636, 1526, 1553, 1622
FriOI	GRGCYC	2 cut(s)	93, 154
Fsp4HI	GCNGC	6 cut(s)	102, 209, 299, 530, 708, 1143
FspBI	CTAG	2 cut(s)	774, 1581
GlaI	GCGC	3 cut(s)	26, 60, 1395
GluI	GCNGC	6 cut(s)	102, 209, 299, 530, 708, 1143
GsuI	CTGGAG	4 cut(s)	56, 570, 867, 972
HaeII	RGCGCY	2 cut(s)	28, 1397
HaeIII	GGCC	4 cut(s)	6, 21, 1453, 1545
HapII	CCGG	5 cut(s)	192, 525, 533, 886, 1546
HgaI	GACGC	2 cut(s)	73, 107
HhaI	GCGC	3 cut(s)	27, 61, 1396
Hin1I	GRCGYC	3 cut(s)	99, 117, 1394
Hin1II	CATG	5 cut(s)	145, 954, 977, 1115, 1679
Hin6I	GCGC	3 cut(s)	25, 59, 1394
HinP1I	GCGC	3 cut(s)	25, 59, 1394
HincII	GTYRAC	3 cut(s)	115, 840, 919
HindII	GTYRAC	3 cut(s)	115, 840, 919
HindIII	AAGCTT	1 cut(s)	560
HinfI	GANTC	3 cut(s)	38, 181, 1467
HpaII	CCGG	5 cut(s)	192, 525, 533, 886, 1546
HphI	GGTGA	5 cut(s)	176, 259, 591, 1598, 1625
Hpy166II	GTNNAC	6 cut(s)	46, 115, 121, 257, 840, 919
Hpy188I	TCNGA	7 cut(s)	161, 180, 220, 278, 1030, 1065, 1156
Hpy188III	TCNNGA	9 cut(s)	147, 344, 508, 578, 846, 886, 989, 1172, 1575
Hpy8I	GTNNAC	6 cut(s)	46, 115, 121, 257, 840, 919
Hpy99I	CGWCG	5 cut(s)	80, 89, 101, 116, 119
HpyAV	CCTTC	6 cut(s)	280, 723, 810, 1013, 1353, 1482
HpyCH4III	ACNGT	6 cut(s)	109, 235, 1057, 1119, 1179, 1460
HpyCH4IV	ACGT	2 cut(s)	117, 592
HpyCH4V	TGCA	7 cut(s)	202, 292, 596, 1232, 1316, 1448, 1519
HpyF10VI	GCNNNNNNNGC	9 cut(s)	22, 31, 208, 298, 322, 497, 683, 1652, 1731
HpyF3I	CTNAG	6 cut(s)	153, 585, 981, 1130, 1260, 1745
HpySE526I	ACGT	2 cut(s)	117, 592
Hsp92I	GRCGYC	3 cut(s)	99, 117, 1394
Hsp92II	CATG	5 cut(s)	145, 954, 977, 1115, 1679
HspAI	GCGC	3 cut(s)	25, 59, 1394
KasI	GGCGCC	1 cut(s)	1393
Kpn2I	TCCGGA	1 cut(s)	885
KroI	GCCGGC	2 cut(s)	191, 532
KroNI	GCCGGC	2 cut(s)	193, 534
Ksp22I	TGATCA	1 cut(s)	1615
LguI	GCTCTTC	1 cut(s)	68
LmnI	GCTCC	8 cut(s)	149, 200, 221, 715, 888, 888, 1174, 1577
Lsp1109I	GCAGC	4 cut(s)	310, 541, 719, 1129
LweI	GCATC	3 cut(s)	508, 605, 695
MaeI	CTAG	2 cut(s)	774, 1581
MaeII	ACGT	2 cut(s)	117, 592
MaeIII	GTNAC	2 cut(s)	1135, 1286
MboII	GAAGA	7 cut(s)	55, 58, 412, 485, 1052, 1504, 1641
MflI	RGATCY	3 cut(s)	161, 1366, 1567
MhlI	GDGCHC	2 cut(s)	93, 154
MlsI	TGGCCA	1 cut(s)	21
MluCI	AATT	7 cut(s)	768, 823, 962, 1214, 1689, 1694, 1700
MluNI	TGGCCA	1 cut(s)	21
Mly113I	GGCGCC	1 cut(s)	1394
MlyI	GAGTC	1 cut(s)	47
MmeI	TCCRAC	1 cut(s)	1344
Mox20I	TGGCCA	1 cut(s)	21
MroI	TCCGGA	1 cut(s)	885
MroNI	GCCGGC	2 cut(s)	191, 532
MscI	TGGCCA	1 cut(s)	21
MseI	TTAA	3 cut(s)	1221, 1239, 1338
MslI	CAYNNNNRTG	1 cut(s)	1281
Msp20I	TGGCCA	1 cut(s)	21
MspI	CCGG	5 cut(s)	192, 525, 533, 886, 1546
MspR9I	CCNGG	4 cut(s)	18, 213, 526, 1547
Mva1269I	GAATGC	1 cut(s)	1000
MvaI	CCWGG	2 cut(s)	18, 213
MvnI	CGCG	2 cut(s)	59, 104
MwoI	GCNNNNNNNGC	9 cut(s)	22, 31, 208, 298, 322, 497, 683, 1652, 1731
NaeI	GCCGGC	2 cut(s)	193, 534
NarI	GGCGCC	1 cut(s)	1394
NciI	CCSGG	2 cut(s)	526, 1547
NdeI	CATATG	1 cut(s)	691
NgoMIV	GCCGGC	2 cut(s)	191, 532
NlaIII	CATG	5 cut(s)	145, 954, 977, 1115, 1679
NlaIV	GGNNCC	4 cut(s)	196, 217, 884, 1395
NmuCI	GTSAC	1 cut(s)	1286
NspI	RCATGY	1 cut(s)	145
PaeR7I	CTCGAG	2 cut(s)	1095, 1386
PceI	AGGCCT	1 cut(s)	1453
PciI	ACATGT	1 cut(s)	141
PciSI	GCTCTTC	1 cut(s)	68
PcsI	WCGNNNNNNNCGW	3 cut(s)	81, 84, 93
PctI	GAATGC	1 cut(s)	1000
PdiI	GCCGGC	2 cut(s)	193, 534
PfeI	GAWTC	2 cut(s)	181, 1467
PflMI	CCANNNNNTGG	1 cut(s)	34
PkrI	GCNGC	6 cut(s)	103, 210, 300, 531, 709, 1144
PleI	GAGTC	1 cut(s)	46
PluTI	GGCGCC	1 cut(s)	1397
PpsI	GAGTC	1 cut(s)	46
PpuMI	RGGWCCY	1 cut(s)	851
PscI	ACATGT	1 cut(s)	141
PshAI	GACNNNNGTC	1 cut(s)	110
PsiI	TTATAA	1 cut(s)	858
Psp124BI	GAGCTC	2 cut(s)	93, 154
Psp5II	RGGWCCY	1 cut(s)	851
Psp6I	CCWGG	2 cut(s)	16, 211
PspGI	CCWGG	2 cut(s)	16, 211
PspN4I	GGNNCC	4 cut(s)	196, 217, 884, 1395
PspPI	GGNCC	2 cut(s)	4, 851
PspPPI	RGGWCCY	1 cut(s)	851
PspXI	VCTCGAGB	2 cut(s)	1095, 1386
PsuI	RGATCY	3 cut(s)	161, 1366, 1567
RsaI	GTAC	2 cut(s)	821, 1718
RsaNI	GTAC	2 cut(s)	820, 1717
RseI	CAYNNNNRTG	1 cut(s)	1281
SacI	GAGCTC	2 cut(s)	93, 154
SalI	GTCGAC	1 cut(s)	113
SapI	GCTCTTC	1 cut(s)	68
SaqAI	TTAA	3 cut(s)	1221, 1239, 1338
SatI	GCNGC	6 cut(s)	102, 209, 299, 530, 708, 1143
Sau96I	GGNCC	2 cut(s)	4, 851
SchI	GAGTC	1 cut(s)	47
ScrFI	CCNGG	4 cut(s)	18, 213, 526, 1547
SduI	GDGCHC	2 cut(s)	93, 154
SfaNI	GCATC	3 cut(s)	508, 605, 695
SfoI	GGCGCC	1 cut(s)	1395
Sfr274I	CTCGAG	2 cut(s)	1095, 1386
SgrDI	CGTCGACG	1 cut(s)	113
SinI	GGWCC	1 cut(s)	851
SlaI	CTCGAG	2 cut(s)	1095, 1386
SmiMI	CAYNNNNRTG	1 cut(s)	1281
SmlI	CTYRAG	2 cut(s)	1095, 1386
SmoI	CTYRAG	2 cut(s)	1095, 1386
Sse9I	AATT	7 cut(s)	768, 823, 962, 1214, 1689, 1694, 1700
SseBI	AGGCCT	1 cut(s)	1453
SsiI	CCGC	3 cut(s)	102, 124, 209
SspDI	GGCGCC	1 cut(s)	1393
SspI	AATATT	3 cut(s)	1014, 1084, 1622
SspMI	CTAG	2 cut(s)	774, 1581
SstI	GAGCTC	2 cut(s)	93, 154
StuI	AGGCCT	1 cut(s)	1453
StyD4I	CCNGG	4 cut(s)	16, 211, 524, 1545
TaaI	ACNGT	6 cut(s)	109, 235, 1057, 1119, 1179, 1460
TaiI	ACGT	2 cut(s)	120, 595
TaqI	TCGA	5 cut(s)	87, 114, 1096, 1387, 1509
TasI	AATT	7 cut(s)	768, 823, 962, 1214, 1689, 1694, 1700
TatI	WGTACW	1 cut(s)	1716
TauI	GCSGC	2 cut(s)	104, 211
TfiI	GAWTC	2 cut(s)	181, 1467
Tru1I	TTAA	3 cut(s)	1221, 1239, 1338
Tru9I	TTAA	3 cut(s)	1221, 1239, 1338
TscAI	CASTG	4 cut(s)	553, 729, 1195, 1526
TseFI	GTSAC	1 cut(s)	1286
TseI	GCWGC	4 cut(s)	298, 529, 707, 1142
Tsp45I	GTSAC	1 cut(s)	1286
TspDTI	ATGAA	7 cut(s)	58, 962, 1128, 1239, 1335, 1459, 1664
TspGWI	ACGGA	2 cut(s)	31, 40
TspRI	CASTG	4 cut(s)	553, 729, 1195, 1526
Van91I	CCANNNNNTGG	1 cut(s)	34
VpaK11BI	GGWCC	1 cut(s)	851
XagI	CCTNNNNNAGG	1 cut(s)	306
XapI	RAATTY	1 cut(s)	1700
XceI	RCATGY	1 cut(s)	145
XcmI	CCANNNNNNNNNTGG	1 cut(s)	14
XhoI	CTCGAG	2 cut(s)	1095, 1386
XmiI	GTMKAC	3 cut(s)	45, 114, 120
XspI	CTAG	2 cut(s)	774, 1581
ZraI	GACGTC	1 cut(s)	118

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.