Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0008595

Physical Location & Seq

Genomic Coordinates Reverse (-)

41897654 .. 41909065

Gene Structure 11412 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ32393

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1695 bp

ATGGGCCATGGGAAGCCTGGCCAGCGCCAAGTGCTGGAGTCCGTCTACTCCGTGATCGCGCTCTTCTTCATTCTCGTCGCCTGCGTCGAGCTCTGTGACGCCGCGACTGTCGTCGACGTCTACCGCCTGATCCAATACGACATGTCGGGAGCTCAGTTCGGATCTCGCCTCGCCAATCTGAATCACCACGCCGGCTCCTTGCACTCCCCTCCTGGCTCCGATCTCTCTCGCACCGTCCTCATCATCCCCTTGCGTGAACTCAACATCACCTTCGTCAGAGAATATATTACGCAGAAGCAGCCTTTGGGAGGCTTGCTGATTTTGCTTCCTCAGATGTTTAGTCCCGAAAATAGAGAAACGGCAAAAAGTAGTCGCGAGACTGATGGAGAGGATGTTTTGAAGAATGTTTTGGCTGAACTTGAAAACCTACTTACACATTCCAAAATACCTTATCCTGTATATTTTGCTTTTGAAGATGATGATATTGAGGCTGTGTTGGCTGATGTCAAGAGGAATGATGCCACAGGGCAGCCTGCTACTGCGACTACTGGAGGATACAAGCTTGTTGTTTCAGTTCCAGAACCTAAGAAACGTGCATCACCCACCATTACAAACATTCAGGGATGGTTGTCAGGGTTGAAAACTGATGGAGACACAAATCAACTTCCAACTATCGCTATTGTAGCATCATATGACACATTTGGGGCAGCTCCTTCTTTATCAGTGGGAAGTGATAGCAATGGAAGTGGGGTTGTCGCCCTACTTGAAATTGCTAGGTTGTTTTCTGTTCTTTACTCAAATCCCAAGACGAGAGGAAGATACAATTTGCTTTTTGGGTTGACATCTGGAGGACCTTATAACTACAATGGAACTAATAAGTGGCTCCGGAGCTTTGACCAACGCCTGCGGGAAAGTGTTGACTACGCTATTTGCTTGAATAGTATTGGCTCATGGGACAAAGAATTGTGGGTTCATGTCTCTAAGCCTCCAGAGAATGCCTACATAAAGCAAATATTTGAAGGTTTCTCTGATGTAGCAGAAGAGTTGGGCATTACAGTTGGTCTGAAGCACAAGAAAATAAATATTTCAAATCCTCGAGTAGCTTGGGAACATGAACAGTTTTCACGGCTGAGAGTTACTGCTGCCACCCTTTCCGAACTCTCTGCTGCTCCTGAACTGTTAGAAAGCACTGGAAGTCTGCATGATGGCAGGCAATTTGTTAATGAAACTGCAATCATTAAGAGTGTCAAGTTAGTTGCTGAGAGCCTTGCGAGGCATATCTATGGTCACAAAGGAAAGAACATTCAAATCTTTGCAGAAAACAGCAGTTTGGCAGTTAATCCTTCATACATAAAATCTTGGTTGGATCTATTGTCGCAAACACCTCGAGTGGCGCCATTTTTATCAAAGAATGATCCATTTATCTTGGCATTGAAAAAGGAATTGGAAGATCATACTGATGAGGTGAATGTGCAGCATGAGGTTCTTGATGGAGTGTTTACTTTCTATGATTCAACTAGGGCTAGCCTTAACATATATCAGGTGGCCAGTGTGACATTTGACTTGTTATTGCTTCTAGTACTGGGATCCTATCTGATAGTGCTCTTCAGTTTTCTTGTCATCACAACTAGGGGTCTTGATGATCTAATCAGTTTATTTCGACGACCTCCCTCACGTAAAGTGAAAACAGCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006355 GO:0006357 GO:0007275 GO:0007399 GO:0008150 GO:0009889 GO:0009966 GO:0009968 GO:0010468 GO:0010556 GO:0010646 GO:0010648 GO:0012505 GO:0016020 GO:0019219 GO:0019222 GO:0023051 GO:0023057 GO:0031323 GO:0031326 GO:0031984 GO:0032501 GO:0032502 GO:0032925 GO:0032926 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048483 GO:0048484 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051171 GO:0051239 GO:0051241 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0090092 GO:0090101 GO:0098827 GO:1900107 GO:1900108 GO:1900145 GO:1900146 GO:1900175 GO:1900176 GO:1903506 GO:2000026 GO:2000112 GO:2000380 GO:2000381 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Nicastrin

Protein Analysis

564

Amino Acids

62.33

Weight (kDa)

6.15

Isoelectric Point (pI)

39.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nicastrin	PF05450	224 - 328	5.8e-06	Nicastrin large lobe

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008595)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G44330
fragaria_vesca	FvH4_3g25670 FvH4_3g25670 FvH4_3g25670 FvH4_3g25670
malus_domestica	MD03G1149800.v1.1 MD11G1168600.v1.1
prunus_persica	Prupe.6G135800_v2.0.a1
pyrus_communis	pycom11g14130
rosa_chinensis	RchiOBHm_Chr5g0045881
rosa_laevigata	RLG00000034394
rosa_multiflora	Rmu_sc0001212.1_g000004
rosa_roxburghii	Rroxscaffold_1G00028740 Rroxscaffold_1G00035230 Rroxscaffold_2G00117520 Rroxscaffold_2G00117530
rosa_rugosa	Rorug05G0226200 Rorug05G0226300
rosa_samantha	Rh5AG307700 Rh5BG316000 Rh5CG342200 Rh5DG326300
rosa_wichuraiana	Rw5G028810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	858
AatII	GACGTC	1 cut(s)	120
AccB1I	GGYRCC	1 cut(s)	1393
AccB7I	CCANNNNNTGG	1 cut(s)	34
AccI	GTMKAC	3 cut(s)	45, 114, 120
AccII	CGCG	3 cut(s)	59, 104, 375
AccIII	TCCGGA	1 cut(s)	885
AciI	CCGC	3 cut(s)	102, 124, 907
AclWI	GGATC	6 cut(s)	124, 169, 1374, 1409, 1581, 1594
AcoI	YGGCCR	2 cut(s)	19, 1545
AcuI	CTGAAG	2 cut(s)	1085, 1591
AcyI	GRCGYC	3 cut(s)	99, 117, 1394
AfaI	GTAC	1 cut(s)	1581
AfiI	CCNNNNNNNGG	2 cut(s)	34, 308
AflIII	ACRYGT	1 cut(s)	141
AjnI	CCWGG	2 cut(s)	16, 211
AjuI	GAANNNNNNNTTGG	6 cut(s)	287, 319, 392, 424, 1427, 1459
AluBI	AGCT	7 cut(s)	91, 152, 562, 710, 891, 1103, 1691
AluI	AGCT	7 cut(s)	91, 152, 562, 710, 891, 1103, 1691
Alw21I	GWGCWC	3 cut(s)	93, 154, 1605
Alw26I	GTCTC	3 cut(s)	371, 645, 982
AlwI	GGATC	6 cut(s)	124, 169, 1374, 1409, 1581, 1594
Ama87I	CYCGRG	2 cut(s)	1095, 1386
Aor13HI	TCCGGA	1 cut(s)	885
AoxI	GGCC	3 cut(s)	4, 19, 1545
ApeKI	GCWGC	6 cut(s)	298, 529, 707, 1142, 1166, 1474
AspLEI	GCGC	3 cut(s)	27, 61, 1396
AspS9I	GGNCC	2 cut(s)	4, 851
AsuHPI	GGTGA	4 cut(s)	176, 259, 591, 1477
AsuNHI	GCTAGC	1 cut(s)	1523
AvaI	CYCGRG	2 cut(s)	1095, 1386
AvaII	GGWCC	1 cut(s)	851
BalI	TGGCCA	2 cut(s)	21, 1547
BamHI	GGATCC	1 cut(s)	1586
BanI	GGYRCC	1 cut(s)	1393
BanII	GRGCYC	2 cut(s)	93, 154
Bbv12I	GWGCWC	3 cut(s)	93, 154, 1605
BbvI	GCAGC	6 cut(s)	310, 541, 719, 1129, 1153, 1486
BccI	CCATC	5 cut(s)	377, 618, 641, 1199, 1484
BceAI	ACGGC	2 cut(s)	375, 1142
BcgI	CGANNNNNNTGC	4 cut(s)	1145, 1179, 1367, 1401
BciT130I	CCWGG	2 cut(s)	18, 213
BciVI	GTATCC	1 cut(s)	548
BcoDI	GTCTC	3 cut(s)	371, 645, 982
BfaI	CTAG	5 cut(s)	774, 1518, 1524, 1577, 1629
BfoI	RGCGCY	2 cut(s)	28, 1397
BfuI	GTATCC	1 cut(s)	548
BisI	GCNGC	7 cut(s)	102, 299, 530, 708, 1143, 1167, 1475
BlsI	GCNGC	7 cut(s)	103, 300, 531, 709, 1144, 1168, 1476
BmcAI	AGTACT	1 cut(s)	1581
Bme1390I	CCNGG	2 cut(s)	18, 213
Bme18I	GGWCC	1 cut(s)	851
BmeT110I	CYCGRG	2 cut(s)	1095, 1386
BmgT120I	GGNCC	2 cut(s)	4, 851
BmiI	GGNNCC	5 cut(s)	196, 217, 884, 1395, 1588
BmrFI	CCNGG	2 cut(s)	18, 213
BmrI	ACTGGG	1 cut(s)	1592
BmsI	GCATC	3 cut(s)	508, 605, 695
BmtI	GCTAGC	1 cut(s)	1527
BmuI	ACTGGG	1 cut(s)	1592
BoxI	GACNNNNGTC	1 cut(s)	110
BpmI	CTGGAG	4 cut(s)	56, 570, 867, 972
BsaAI	YACGTR	1 cut(s)	1676
BsaHI	GRCGYC	3 cut(s)	99, 117, 1394
BsaJI	CCNNGG	1 cut(s)	7
BsaWI	WCCGGW	1 cut(s)	885
BsaXI	ACNNNNNCTCC	4 cut(s)	179, 209, 378, 408
Bsc4I	CCNNNNNNNGG	2 cut(s)	34, 308
Bse118I	RCCGGY	1 cut(s)	191
Bse1I	ACTGG	4 cut(s)	553, 1195, 1548, 1587
Bse3DI	GCAATG	1 cut(s)	745
BseAI	TCCGGA	1 cut(s)	885
BseBI	CCWGG	2 cut(s)	18, 213
BseDI	CCNNGG	1 cut(s)	7
BseGI	GGATG	3 cut(s)	243, 397, 629
BseLI	CCNNNNNNNGG	2 cut(s)	34, 308
BseMI	GCAATG	1 cut(s)	745
BseMII	CTCAG	4 cut(s)	167, 344, 1121, 1251
BseNI	ACTGG	4 cut(s)	553, 1195, 1548, 1587
BseXI	GCAGC	6 cut(s)	310, 541, 719, 1129, 1153, 1486
BsgI	GTGCAG	1 cut(s)	1493
Bsh1236I	CGCG	3 cut(s)	59, 104, 375
BshFI	GGCC	3 cut(s)	6, 21, 1547
BshNI	GGYRCC	1 cut(s)	1393
BsiHKAI	GWGCWC	3 cut(s)	93, 154, 1605
BsiHKCI	CYCGRG	2 cut(s)	1095, 1386
BsiSI	CCGG	2 cut(s)	192, 886
BslFI	GGGAC	2 cut(s)	327, 968
BslI	CCNNNNNNNGG	2 cut(s)	34, 308
BsmAI	GTCTC	3 cut(s)	371, 645, 982
BsmFI	GGGAC	2 cut(s)	327, 968
BsmI	GAATGC	1 cut(s)	1000
BsnI	GGCC	3 cut(s)	6, 21, 1547
BsoBI	CYCGRG	2 cut(s)	1095, 1386
Bsp1286I	GDGCHC	3 cut(s)	93, 154, 1605
Bsp13I	TCCGGA	1 cut(s)	885
Bsp143I	GATC	9 cut(s)	54, 129, 161, 220, 1366, 1414, 1450, 1586, 1642
Bsp19I	CCATGG	1 cut(s)	7
Bsp68I	TCGCGA	1 cut(s)	375
BspACI	CCGC	3 cut(s)	102, 124, 907
BspANI	GGCC	3 cut(s)	6, 21, 1547
BspCNI	CTCAG	4 cut(s)	166, 343, 1122, 1252
BspEI	TCCGGA	1 cut(s)	885
BspFNI	CGCG	3 cut(s)	59, 104, 375
BspLI	GGNNCC	5 cut(s)	196, 217, 884, 1395, 1588
BspOI	GCTAGC	1 cut(s)	1527
BspPI	GGATC	6 cut(s)	124, 169, 1374, 1409, 1581, 1594
BspQI	GCTCTTC	2 cut(s)	68, 1610
BspT107I	GGYRCC	1 cut(s)	1393
BsrDI	GCAATG	1 cut(s)	745
BsrFI	RCCGGY	1 cut(s)	191
BsrI	ACTGG	4 cut(s)	553, 1195, 1548, 1587
BssAI	RCCGGY	1 cut(s)	191
BssECI	CCNNGG	1 cut(s)	7
BssMI	GATC	9 cut(s)	54, 129, 161, 220, 1366, 1414, 1450, 1586, 1642
BssNI	GRCGYC	3 cut(s)	99, 117, 1394
BssT1I	CCWWGG	1 cut(s)	7
Bst2UI	CCWGG	2 cut(s)	18, 213
Bst4CI	ACNGT	5 cut(s)	109, 235, 1057, 1119, 1179
Bst6I	CTCTTC	3 cut(s)	68, 1035, 1610
BstACI	GRCGYC	3 cut(s)	99, 117, 1394
BstBAI	YACGTR	1 cut(s)	1676
BstC8I	GCNNGC	8 cut(s)	23, 82, 193, 314, 534, 905, 1211, 1525
BstDEI	CTNAG	6 cut(s)	153, 330, 585, 981, 1130, 1260
BstDSI	CCRYGG	1 cut(s)	7
BstENI	CCTNNNNNAGG	1 cut(s)	306
BstF5I	GGATG	3 cut(s)	243, 397, 629
BstFNI	CGCG	3 cut(s)	59, 104, 375
BstH2I	RGCGCY	2 cut(s)	28, 1397
BstHHI	GCGC	3 cut(s)	27, 61, 1396
BstKTI	GATC	9 cut(s)	57, 132, 164, 223, 1369, 1417, 1453, 1589, 1645
BstMAI	GTCTC	3 cut(s)	371, 645, 982
BstMBI	GATC	9 cut(s)	54, 129, 161, 220, 1366, 1414, 1450, 1586, 1642
BstMWI	GCNNNNNNNGC	6 cut(s)	22, 31, 298, 322, 497, 683
BstNI	CCWGG	2 cut(s)	18, 213
BstNSI	RCATGY	1 cut(s)	145
BstPAI	GACNNNNGTC	1 cut(s)	110
BstSCI	CCNGG	2 cut(s)	16, 211
BstUI	CGCG	3 cut(s)	59, 104, 375
BstV1I	GCAGC	6 cut(s)	310, 541, 719, 1129, 1153, 1486
BstX2I	RGATCY	3 cut(s)	161, 1366, 1586
BstYI	RGATCY	3 cut(s)	161, 1366, 1586
BsuI	GTATCC	1 cut(s)	548
BsuRI	GGCC	3 cut(s)	6, 21, 1547
BtgI	CCRYGG	1 cut(s)	7
BtsCI	GGATG	3 cut(s)	243, 397, 629
BtsIMutI	CAGTG	3 cut(s)	729, 1188, 1555
BtuMI	TCGCGA	1 cut(s)	375
Cac8I	GCNNGC	8 cut(s)	23, 82, 193, 314, 534, 905, 1211, 1525
CfoI	GCGC	3 cut(s)	27, 61, 1396
Cfr10I	RCCGGY	1 cut(s)	191
Cfr13I	GGNCC	2 cut(s)	4, 851
CseI	GACGC	2 cut(s)	73, 107
Csp6I	GTAC	1 cut(s)	1580
CspCI	CAANNNNNGTGG	2 cut(s)	727, 762
CviAII	CATG	7 cut(s)	8, 142, 951, 974, 1112, 1202, 1478
CviQI	GTAC	1 cut(s)	1580
DdeI	CTNAG	6 cut(s)	153, 330, 585, 981, 1130, 1260
DinI	GGCGCC	1 cut(s)	1395
DpnI	GATC	9 cut(s)	56, 131, 163, 222, 1368, 1416, 1452, 1588, 1644
DpnII	GATC	9 cut(s)	54, 129, 161, 220, 1366, 1414, 1450, 1586, 1642
EaeI	YGGCCR	2 cut(s)	19, 1545
Eam1104I	CTCTTC	3 cut(s)	68, 1035, 1610
EarI	CTCTTC	3 cut(s)	68, 1035, 1610
Ecl136II	GAGCTC	2 cut(s)	91, 152
Eco130I	CCWWGG	1 cut(s)	7
Eco24I	GRGCYC	2 cut(s)	93, 154
Eco47I	GGWCC	1 cut(s)	851
Eco53kI	GAGCTC	2 cut(s)	91, 152
Eco57I	CTGAAG	2 cut(s)	1085, 1591
Eco88I	CYCGRG	2 cut(s)	1095, 1386
EcoICRI	GAGCTC	2 cut(s)	91, 152
EcoNI	CCTNNNNNAGG	1 cut(s)	306
EcoO109I	RGGNCCY	1 cut(s)	851
EcoRII	CCWGG	2 cut(s)	16, 211
EcoT14I	CCWWGG	1 cut(s)	7
EcoT38I	GRGCYC	2 cut(s)	93, 154
EgeI	GGCGCC	1 cut(s)	1395
EheI	GGCGCC	1 cut(s)	1395
ErhI	CCWWGG	1 cut(s)	7
FaeI	CATG	7 cut(s)	11, 145, 954, 977, 1115, 1205, 1481
FaqI	GGGAC	2 cut(s)	327, 968
FatI	CATG	7 cut(s)	7, 141, 950, 973, 1111, 1201, 1477
FauI	CCCGC	1 cut(s)	900
FauNDI	CATATG	1 cut(s)	691
FblI	GTMKAC	3 cut(s)	45, 114, 120
Fnu4HI	GCNGC	7 cut(s)	102, 299, 530, 708, 1143, 1167, 1475
FokI	GGATG	3 cut(s)	230, 404, 636
FriOI	GRGCYC	2 cut(s)	93, 154
Fsp4HI	GCNGC	7 cut(s)	102, 299, 530, 708, 1143, 1167, 1475
FspBI	CTAG	5 cut(s)	774, 1518, 1524, 1577, 1629
GlaI	GCGC	3 cut(s)	26, 60, 1395
GluI	GCNGC	7 cut(s)	102, 299, 530, 708, 1143, 1167, 1475
GsuI	CTGGAG	4 cut(s)	56, 570, 867, 972
HaeII	RGCGCY	2 cut(s)	28, 1397
HaeIII	GGCC	3 cut(s)	6, 21, 1547
HapII	CCGG	2 cut(s)	192, 886
HgaI	GACGC	2 cut(s)	73, 107
HhaI	GCGC	3 cut(s)	27, 61, 1396
Hin1I	GRCGYC	3 cut(s)	99, 117, 1394
Hin1II	CATG	7 cut(s)	11, 145, 954, 977, 1115, 1205, 1481
Hin6I	GCGC	3 cut(s)	25, 59, 1394
HinP1I	GCGC	3 cut(s)	25, 59, 1394
HincII	GTYRAC	3 cut(s)	115, 840, 919
HindII	GTYRAC	3 cut(s)	115, 840, 919
HindIII	AAGCTT	1 cut(s)	560
HinfI	GANTC	3 cut(s)	38, 181, 1511
HpaII	CCGG	2 cut(s)	192, 886
HphI	GGTGA	4 cut(s)	176, 259, 591, 1477
Hpy166II	GTNNAC	7 cut(s)	46, 115, 121, 257, 840, 919, 1500
Hpy188I	TCNGA	9 cut(s)	161, 180, 220, 278, 333, 1030, 1065, 1156, 1596
Hpy8I	GTNNAC	7 cut(s)	46, 115, 121, 257, 840, 919, 1500
Hpy99I	CGWCG	5 cut(s)	80, 89, 116, 119, 1665
HpyAV	CCTTC	4 cut(s)	280, 723, 1013, 1353
HpyCH4III	ACNGT	5 cut(s)	109, 235, 1057, 1119, 1179
HpyCH4IV	ACGT	3 cut(s)	117, 592, 1675
HpyCH4V	TGCA	6 cut(s)	202, 596, 1201, 1232, 1316, 1474
HpyF10VI	GCNNNNNNNGC	6 cut(s)	22, 31, 298, 322, 497, 683
HpyF3I	CTNAG	6 cut(s)	153, 330, 585, 981, 1130, 1260
HpySE526I	ACGT	3 cut(s)	117, 592, 1675
Hsp92I	GRCGYC	3 cut(s)	99, 117, 1394
Hsp92II	CATG	7 cut(s)	11, 145, 954, 977, 1115, 1205, 1481
HspAI	GCGC	3 cut(s)	25, 59, 1394
KasI	GGCGCC	1 cut(s)	1393
Kpn2I	TCCGGA	1 cut(s)	885
KroI	GCCGGC	1 cut(s)	191
KroNI	GCCGGC	1 cut(s)	193
Kzo9I	GATC	9 cut(s)	54, 129, 161, 220, 1366, 1414, 1450, 1586, 1642
LguI	GCTCTTC	2 cut(s)	68, 1610
LmnI	GCTCC	7 cut(s)	149, 200, 221, 715, 888, 888, 1174
Lsp1109I	GCAGC	6 cut(s)	310, 541, 719, 1129, 1153, 1486
LweI	GCATC	3 cut(s)	508, 605, 695
MaeI	CTAG	5 cut(s)	774, 1518, 1524, 1577, 1629
MaeII	ACGT	3 cut(s)	117, 592, 1675
MaeIII	GTNAC	4 cut(s)	95, 1135, 1286, 1552
MalI	GATC	9 cut(s)	56, 131, 163, 222, 1368, 1416, 1452, 1588, 1644
MboI	GATC	9 cut(s)	54, 129, 161, 220, 1366, 1414, 1450, 1586, 1642
MboII	GAAGA	8 cut(s)	55, 58, 412, 485, 828, 1052, 1460, 1597
MflI	RGATCY	3 cut(s)	161, 1366, 1586
MhlI	GDGCHC	3 cut(s)	93, 154, 1605
MlsI	TGGCCA	2 cut(s)	21, 1547
MluCI	AATT	5 cut(s)	768, 823, 962, 1214, 1442
MluNI	TGGCCA	2 cut(s)	21, 1547
Mly113I	GGCGCC	1 cut(s)	1394
MlyI	GAGTC	1 cut(s)	47
MmeI	TCCRAC	2 cut(s)	692, 1344
Mox20I	TGGCCA	2 cut(s)	21, 1547
MroI	TCCGGA	1 cut(s)	885
MroNI	GCCGGC	1 cut(s)	191
MscI	TGGCCA	2 cut(s)	21, 1547
MseI	TTAA	4 cut(s)	1221, 1239, 1338, 1530
MslI	CAYNNNNRTG	2 cut(s)	1281, 1458
Msp20I	TGGCCA	2 cut(s)	21, 1547
MspI	CCGG	2 cut(s)	192, 886
MspR9I	CCNGG	2 cut(s)	18, 213
Mva1269I	GAATGC	1 cut(s)	1000
MvaI	CCWGG	2 cut(s)	18, 213
MvnI	CGCG	3 cut(s)	59, 104, 375
MwoI	GCNNNNNNNGC	6 cut(s)	22, 31, 298, 322, 497, 683
NaeI	GCCGGC	1 cut(s)	193
NarI	GGCGCC	1 cut(s)	1394
NcoI	CCATGG	1 cut(s)	7
NdeI	CATATG	1 cut(s)	691
NdeII	GATC	9 cut(s)	54, 129, 161, 220, 1366, 1414, 1450, 1586, 1642
NgoMIV	GCCGGC	1 cut(s)	191
NheI	GCTAGC	1 cut(s)	1523
NlaIII	CATG	7 cut(s)	11, 145, 954, 977, 1115, 1205, 1481
NlaIV	GGNNCC	5 cut(s)	196, 217, 884, 1395, 1588
NmuCI	GTSAC	3 cut(s)	95, 1286, 1552
NruI	TCGCGA	1 cut(s)	375
NspI	RCATGY	1 cut(s)	145
PaeR7I	CTCGAG	2 cut(s)	1095, 1386
PciI	ACATGT	1 cut(s)	141
PciSI	GCTCTTC	2 cut(s)	68, 1610
PcsI	WCGNNNNNNNCGW	2 cut(s)	81, 84
PctI	GAATGC	1 cut(s)	1000
PdiI	GCCGGC	1 cut(s)	193
PfeI	GAWTC	2 cut(s)	181, 1511
PflMI	CCANNNNNTGG	1 cut(s)	34
PkrI	GCNGC	7 cut(s)	103, 300, 531, 709, 1144, 1168, 1476
PleI	GAGTC	1 cut(s)	46
PluTI	GGCGCC	1 cut(s)	1397
PpsI	GAGTC	1 cut(s)	46
Ppu21I	YACGTR	1 cut(s)	1676
PpuMI	RGGWCCY	1 cut(s)	851
PscI	ACATGT	1 cut(s)	141
PshAI	GACNNNNGTC	1 cut(s)	110
PsiI	TTATAA	1 cut(s)	858
Psp124BI	GAGCTC	2 cut(s)	93, 154
Psp5II	RGGWCCY	1 cut(s)	851
Psp6I	CCWGG	2 cut(s)	16, 211
PspGI	CCWGG	2 cut(s)	16, 211
PspN4I	GGNNCC	5 cut(s)	196, 217, 884, 1395, 1588
PspPI	GGNCC	2 cut(s)	4, 851
PspPPI	RGGWCCY	1 cut(s)	851
PspXI	VCTCGAGB	2 cut(s)	1095, 1386
PsuI	RGATCY	3 cut(s)	161, 1366, 1586
RruI	TCGCGA	1 cut(s)	375
RsaI	GTAC	1 cut(s)	1581
RsaNI	GTAC	1 cut(s)	1580
RseI	CAYNNNNRTG	2 cut(s)	1281, 1458
SacI	GAGCTC	2 cut(s)	93, 154
SalI	GTCGAC	1 cut(s)	113
SapI	GCTCTTC	2 cut(s)	68, 1610
SaqAI	TTAA	4 cut(s)	1221, 1239, 1338, 1530
SatI	GCNGC	7 cut(s)	102, 299, 530, 708, 1143, 1167, 1475
Sau3AI	GATC	9 cut(s)	54, 129, 161, 220, 1366, 1414, 1450, 1586, 1642
Sau96I	GGNCC	2 cut(s)	4, 851
ScaI	AGTACT	1 cut(s)	1581
SchI	GAGTC	1 cut(s)	47
ScrFI	CCNGG	2 cut(s)	18, 213
SduI	GDGCHC	3 cut(s)	93, 154, 1605
SfaNI	GCATC	3 cut(s)	508, 605, 695
SfoI	GGCGCC	1 cut(s)	1395
Sfr274I	CTCGAG	2 cut(s)	1095, 1386
SgrDI	CGTCGACG	1 cut(s)	113
SinI	GGWCC	1 cut(s)	851
SlaI	CTCGAG	2 cut(s)	1095, 1386
SmiMI	CAYNNNNRTG	2 cut(s)	1281, 1458
SmlI	CTYRAG	2 cut(s)	1095, 1386
SmoI	CTYRAG	2 cut(s)	1095, 1386
Sse9I	AATT	5 cut(s)	768, 823, 962, 1214, 1442
SsiI	CCGC	3 cut(s)	102, 124, 907
SspDI	GGCGCC	1 cut(s)	1393
SspI	AATATT	2 cut(s)	1014, 1084
SspMI	CTAG	5 cut(s)	774, 1518, 1524, 1577, 1629
SstI	GAGCTC	2 cut(s)	93, 154
StyD4I	CCNGG	2 cut(s)	16, 211
StyI	CCWWGG	1 cut(s)	7
TaaI	ACNGT	5 cut(s)	109, 235, 1057, 1119, 1179
TaiI	ACGT	3 cut(s)	120, 595, 1678
TaqI	TCGA	5 cut(s)	87, 114, 1096, 1387, 1660
TasI	AATT	5 cut(s)	768, 823, 962, 1214, 1442
TatI	WGTACW	1 cut(s)	1579
TauI	GCSGC	1 cut(s)	104
TfiI	GAWTC	2 cut(s)	181, 1511
Tru1I	TTAA	4 cut(s)	1221, 1239, 1338, 1530
Tru9I	TTAA	4 cut(s)	1221, 1239, 1338, 1530
TscAI	CASTG	3 cut(s)	729, 1195, 1555
TseFI	GTSAC	3 cut(s)	95, 1286, 1552
TseI	GCWGC	6 cut(s)	298, 529, 707, 1142, 1166, 1474
Tsp45I	GTSAC	3 cut(s)	95, 1286, 1552
TspDTI	ATGAA	5 cut(s)	58, 962, 1128, 1239, 1335
TspGWI	ACGGA	2 cut(s)	31, 40
TspRI	CASTG	3 cut(s)	729, 1195, 1555
Van91I	CCANNNNNTGG	1 cut(s)	34
VpaK11BI	GGWCC	1 cut(s)	851
XagI	CCTNNNNNAGG	1 cut(s)	306
XceI	RCATGY	1 cut(s)	145
XcmI	CCANNNNNNNNNTGG	1 cut(s)	14
XhoI	CTCGAG	2 cut(s)	1095, 1386
XmiI	GTMKAC	3 cut(s)	45, 114, 120
XspI	CTAG	5 cut(s)	774, 1518, 1524, 1577, 1629
ZraI	GACGTC	1 cut(s)	118
ZrmI	AGTACT	1 cut(s)	1581

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.