MD03G1150500.v1.1

photosystem I subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015925
Physical Location & Seq
Forward (+)
16817720 .. 16821328
3609 bp
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UTR
Exon/CDS
Intron
MD03G1150500.v1.1.491

Sequence Viewer

Length: 432 bp
ATGGCAGTAAGCATGGCGACCGCATCAACCGTGATTGGGCTGGGTAGTTCCTCCCTCTCACCAAACAGGACATGCCTTACTTCAGGCTTTGTGAAGCCTATCGTGGTTGGAAACCCTTTGAGGCAAGTTAGGGCTTCTGGAGGAAGGTTCACATGCTTTCAGAGGGATTGGCTGCGGAGAGATCTGAATGTGATTGGGTTTGGGTTGATAGGATGGCTGGCTCCGTCGAGCATACCGTTAATCGACGGTAAGAGTTTGACGGGGCTCTTCTTTGAGAGCATTGGTGCTGAACTCGCTCACTTCCCCTCACCTCCTGCTCTCACTTCTCAGTTTTGGCTGTGGCTGATAACATGGCACTTGGGGCTTTTCATCACCCTGACCTTTGGGCAGATTGGATTCAAGGGGAGGACTGAAGATTACTTTGATAAATGA

Protein Analysis

144

Amino Acids

15.65

Weight (kDa)

9.58

Isoelectric Point (pI)

44.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsaO_TMD PF22832 53 - 141 2.6e-43 PsaO transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015925)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08380
fragaria_vesca FvH4_3g25770
malus_domestica MD03G1150500.v1.1 MD11G1169900.v1.1
prunus_persica Prupe.6G137400_v2.0.a1
pyrus_communis pycom03g10460 pycom11g14230
rosa_chinensis RchiOBHm_Chr5g0046981
rosa_laevigata RLG00000034456
rosa_multiflora Rmu_sc0005544.1_g000002
rosa_roxburghii Rroxscaffold_1G00034440
rosa_rugosa Rorug05G0233100
rosa_samantha Rh5BG321400 Rh5CG347500
rosa_wichuraiana Rw5G029190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 21, 175
AcuI CTGAAG 2 cut(s) 66, 432
AfiI CCNNNNNNNGG 1 cut(s) 36
AgsI TTSAA 1 cut(s) 400
ApeKI GCWGC 1 cut(s) 172
AsuHPI GGTGA 3 cut(s) 51, 300, 364
BanII GRGCYC 1 cut(s) 267
BbvI GCAGC 1 cut(s) 159
BccI CCATC 1 cut(s) 207
BglII AGATCT 1 cut(s) 181
BisI GCNGC 1 cut(s) 173
BlsI GCNGC 1 cut(s) 174
BmiI GGNNCC 1 cut(s) 222
BmsI GCATC 1 cut(s) 32
BpmI CTGGAG 1 cut(s) 159
Bsc4I CCNNNNNNNGG 1 cut(s) 36
BseGI GGATG 1 cut(s) 218
BseLI CCNNNNNNNGG 1 cut(s) 36
BseMII CTCAG 1 cut(s) 341
BseXI GCAGC 1 cut(s) 159
BseYI CCCAGC 1 cut(s) 40
Bsh1285I CGRYCG 1 cut(s) 21
BsiEI CGRYCG 1 cut(s) 21
BslI CCNNNNNNNGG 1 cut(s) 36
Bsp1286I GDGCHC 1 cut(s) 267
Bsp143I GATC 1 cut(s) 181
BspACI CCGC 2 cut(s) 21, 175
BspCNI CTCAG 1 cut(s) 340
BspLI GGNNCC 1 cut(s) 222
BspQI GCTCTTC 1 cut(s) 272
BssMI GATC 1 cut(s) 181
Bst4CI ACNGT 3 cut(s) 31, 237, 248
Bst6I CTCTTC 1 cut(s) 272
BstC8I GCNNGC 1 cut(s) 219
BstDEI CTNAG 1 cut(s) 327
BstF5I GGATG 1 cut(s) 218
BstKTI GATC 1 cut(s) 184
BstMBI GATC 1 cut(s) 181
BstMCI CGRYCG 1 cut(s) 21
BstMWI GCNNNNNNNGC 2 cut(s) 293, 361
BstNSI RCATGY 2 cut(s) 75, 156
BstV1I GCAGC 1 cut(s) 159
BstX2I RGATCY 1 cut(s) 181
BstYI RGATCY 1 cut(s) 181
BtsCI GGATG 1 cut(s) 218
Cac8I GCNNGC 1 cut(s) 219
CviAII CATG 4 cut(s) 13, 72, 153, 351
DdeI CTNAG 1 cut(s) 327
DpnI GATC 1 cut(s) 183
DpnII GATC 1 cut(s) 181
Eam1104I CTCTTC 1 cut(s) 272
EarI CTCTTC 1 cut(s) 272
Eco24I GRGCYC 1 cut(s) 267
Eco57I CTGAAG 2 cut(s) 66, 432
EcoT38I GRGCYC 1 cut(s) 267
FaeI CATG 4 cut(s) 16, 75, 156, 354
FaiI YATR 5 cut(s) 14, 73, 154, 233, 352
FatI CATG 4 cut(s) 12, 71, 152, 350
Fnu4HI GCNGC 1 cut(s) 173
FokI GGATG 1 cut(s) 225
FriOI GRGCYC 1 cut(s) 267
Fsp4HI GCNGC 1 cut(s) 173
GluI GCNGC 1 cut(s) 173
GsaI CCCAGC 1 cut(s) 44
GsuI CTGGAG 1 cut(s) 159
Hin1II CATG 4 cut(s) 16, 75, 156, 354
HinfI GANTC 1 cut(s) 396
HphI GGTGA 3 cut(s) 51, 300, 364
Hpy166II GTNNAC 1 cut(s) 150
Hpy188I TCNGA 2 cut(s) 162, 186
Hpy188III TCNNGA 1 cut(s) 138
Hpy8I GTNNAC 1 cut(s) 150
Hpy99I CGWCG 2 cut(s) 229, 248
HpyAV CCTTC 1 cut(s) 138
HpyCH4III ACNGT 3 cut(s) 31, 237, 248
HpyF10VI GCNNNNNNNGC 2 cut(s) 293, 361
HpyF3I CTNAG 1 cut(s) 327
Hsp92II CATG 4 cut(s) 16, 75, 156, 354
Kzo9I GATC 1 cut(s) 181
LguI GCTCTTC 1 cut(s) 272
LmnI GCTCC 1 cut(s) 226
LpnPI CCDG 7 cut(s) 26, 52, 69, 123, 203, 327, 389
Lsp1109I GCAGC 1 cut(s) 159
LweI GCATC 1 cut(s) 32
MalI GATC 1 cut(s) 183
MboI GATC 1 cut(s) 181
MboII GAAGA 2 cut(s) 259, 425
MflI RGATCY 1 cut(s) 181
MhlI GDGCHC 1 cut(s) 267
MmeI TCCRAC 1 cut(s) 88
MnlI CCTC 8 cut(s) 61, 65, 114, 134, 156, 316, 321, 399
MseI TTAA 1 cut(s) 239
MwoI GCNNNNNNNGC 2 cut(s) 293, 361
NdeII GATC 1 cut(s) 181
NlaIII CATG 4 cut(s) 16, 75, 156, 354
NlaIV GGNNCC 1 cut(s) 222
NspI RCATGY 2 cut(s) 75, 156
PciSI GCTCTTC 1 cut(s) 272
PcsI WCGNNNNNNNCGW 1 cut(s) 233
PfeI GAWTC 1 cut(s) 396
PkrI GCNGC 1 cut(s) 174
PspFI CCCAGC 1 cut(s) 40
PspN4I GGNNCC 1 cut(s) 222
PsuI RGATCY 1 cut(s) 181
SapI GCTCTTC 1 cut(s) 272
SaqAI TTAA 1 cut(s) 239
SatI GCNGC 1 cut(s) 173
Sau3AI GATC 1 cut(s) 181
SduI GDGCHC 1 cut(s) 267
SetI ASST 3 cut(s) 149, 313, 383
SfaNI GCATC 1 cut(s) 32
SsiI CCGC 2 cut(s) 21, 175
TaaI ACNGT 3 cut(s) 31, 237, 248
TaqI TCGA 2 cut(s) 227, 243
TfiI GAWTC 1 cut(s) 396
Tru1I TTAA 1 cut(s) 239
Tru9I TTAA 1 cut(s) 239
TseI GCWGC 1 cut(s) 172
TspDTI ATGAA 1 cut(s) 358
TspGWI ACGGA 1 cut(s) 213
XceI RCATGY 2 cut(s) 75, 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.