RLG00000034456

photosystem I subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015925
Physical Location & Seq
Forward (+)
42993330 .. 42994377
1048 bp
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UTR
Exon/CDS
Intron
RLM00000034456

Sequence Viewer

Length: 429 bp
ATGGCGACAAGCTTCGCAACCGCATCGACCGTCGTAGGCCTTGGAAGCTCGCTCTCGTCCAAGAGGACATGCCTCTCTTCAGACTTTGTGAAGCCTGTCATGGTTAGGAGGCCTCTGAGCCAAGCAAAAGCTTCTGGAGGAAGGTTCACCTGCATGAGCTTCCAGAGTGATTGGCTGAGGAGGGATCTGAATGTGATCGGGTTCGGGTTGATCGGATGGTTGGTGCCATCGAGCGTTCCGGCGATTGACGGCAAGAGTTTGACCGGGCTCTTCTTTGAGAGCATTGGAGCCGAGCTCGCTCACTTCCCCACACCTCCAGCTCTCACTTCACAGTTCTGGTTGTGGTTGATCTGCTGGCACTTGGGGCTTTTCATCACCCTTACTTTTGGGCAGATTGGCTTCAAGGGAAGGACCGACAAGTACTTTTGA

Protein Analysis

143

Amino Acids

15.54

Weight (kDa)

9.73

Isoelectric Point (pI)

57.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsaO_TMD PF22832 54 - 142 3.2e-43 PsaO transmembrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015925)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08380
fragaria_vesca FvH4_3g25770
malus_domestica MD03G1150500.v1.1 MD11G1169900.v1.1
prunus_persica Prupe.6G137400_v2.0.a1
pyrus_communis pycom03g10460 pycom11g14230
rosa_chinensis RchiOBHm_Chr5g0046981
rosa_laevigata RLG00000034456
rosa_multiflora Rmu_sc0005544.1_g000002
rosa_roxburghii Rroxscaffold_1G00034440
rosa_rugosa Rorug05G0233100
rosa_samantha Rh5BG321400 Rh5CG347500
rosa_wichuraiana Rw5G029190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 158
Acc36I ACCTGC 1 cut(s) 158
AccB1I GGYRCC 1 cut(s) 223
AciI CCGC 1 cut(s) 21
AclWI GGATC 1 cut(s) 192
AcuI CTGAAG 1 cut(s) 63
AfaI GTAC 1 cut(s) 422
AgsI TTSAA 1 cut(s) 403
AluBI AGCT 6 cut(s) 12, 48, 131, 159, 295, 320
AluI AGCT 6 cut(s) 12, 48, 131, 159, 295, 320
Alw21I GWGCWC 1 cut(s) 297
AlwI GGATC 1 cut(s) 192
AoxI GGCC 2 cut(s) 37, 110
AspS9I GGNCC 1 cut(s) 411
AsuC2I CCSGG 1 cut(s) 265
AsuHPI GGTGA 2 cut(s) 139, 367
AvaII GGWCC 1 cut(s) 411
BanI GGYRCC 1 cut(s) 223
BanII GRGCYC 2 cut(s) 270, 297
Bbv12I GWGCWC 1 cut(s) 297
BbvCI CCTCAGC 1 cut(s) 176
BccI CCATC 2 cut(s) 210, 235
BceAI ACGGC 1 cut(s) 265
BcgI CGANNNNNNTGC 2 cut(s) 6, 40
BcnI CCSGG 1 cut(s) 265
BfuAI ACCTGC 1 cut(s) 158
BmcAI AGTACT 1 cut(s) 422
Bme1390I CCNGG 1 cut(s) 265
Bme18I GGWCC 1 cut(s) 411
BmgT120I GGNCC 1 cut(s) 411
BmiI GGNNCC 2 cut(s) 225, 289
BmrFI CCNGG 1 cut(s) 265
BmsI GCATC 1 cut(s) 32
BplI GAGNNNNNCTC 2 cut(s) 279, 311
BpmI CTGGAG 2 cut(s) 156, 300
Bpu10I CCTNAGC 1 cut(s) 176
BpuMI CCSGG 1 cut(s) 265
BsaJI CCNNGG 1 cut(s) 40
BseDI CCNNGG 1 cut(s) 40
BseGI GGATG 1 cut(s) 221
BseMII CTCAG 2 cut(s) 107, 167
BseRI GAGGAG 1 cut(s) 193
Bsh1285I CGRYCG 1 cut(s) 30
BshFI GGCC 2 cut(s) 39, 112
BshNI GGYRCC 1 cut(s) 223
BsiEI CGRYCG 1 cut(s) 30
BsiHKAI GWGCWC 1 cut(s) 297
BsiSI CCGG 2 cut(s) 239, 264
BsnI GGCC 2 cut(s) 39, 112
Bsp1286I GDGCHC 2 cut(s) 270, 297
Bsp143I GATC 4 cut(s) 184, 195, 210, 348
BspACI CCGC 1 cut(s) 21
BspANI GGCC 2 cut(s) 39, 112
BspCNI CTCAG 2 cut(s) 108, 168
BspLI GGNNCC 2 cut(s) 225, 289
BspMI ACCTGC 1 cut(s) 158
BspPI GGATC 1 cut(s) 192
BspQI GCTCTTC 1 cut(s) 275
BspT107I GGYRCC 1 cut(s) 223
BssECI CCNNGG 1 cut(s) 40
BssMI GATC 4 cut(s) 184, 195, 210, 348
BssT1I CCWWGG 1 cut(s) 40
Bst4CI ACNGT 2 cut(s) 31, 333
Bst6I CTCTTC 2 cut(s) 82, 275
BstC8I GCNNGC 3 cut(s) 50, 297, 356
BstDEI CTNAG 2 cut(s) 116, 176
BstF5I GGATG 1 cut(s) 221
BstKTI GATC 4 cut(s) 187, 198, 213, 351
BstMBI GATC 4 cut(s) 184, 195, 210, 348
BstMCI CGRYCG 1 cut(s) 30
BstMWI GCNNNNNNNGC 3 cut(s) 45, 296, 364
BstNSI RCATGY 1 cut(s) 72
BstSCI CCNGG 1 cut(s) 263
BstX2I RGATCY 1 cut(s) 184
BstYI RGATCY 1 cut(s) 184
BsuRI GGCC 2 cut(s) 39, 112
BtsCI GGATG 1 cut(s) 221
BveI ACCTGC 1 cut(s) 158
Cac8I GCNNGC 3 cut(s) 50, 297, 356
Cfr13I GGNCC 1 cut(s) 411
Csp6I GTAC 1 cut(s) 421
CviAII CATG 3 cut(s) 69, 100, 154
CviQI GTAC 1 cut(s) 421
DdeI CTNAG 2 cut(s) 116, 176
DpnI GATC 4 cut(s) 186, 197, 212, 350
DpnII GATC 4 cut(s) 184, 195, 210, 348
Eam1104I CTCTTC 2 cut(s) 82, 275
EarI CTCTTC 2 cut(s) 82, 275
Ecl136II GAGCTC 1 cut(s) 295
Eco130I CCWWGG 1 cut(s) 40
Eco147I AGGCCT 2 cut(s) 39, 112
Eco24I GRGCYC 2 cut(s) 270, 297
Eco47I GGWCC 1 cut(s) 411
Eco53kI GAGCTC 1 cut(s) 295
Eco57I CTGAAG 1 cut(s) 63
EcoICRI GAGCTC 1 cut(s) 295
EcoT14I CCWWGG 1 cut(s) 40
EcoT38I GRGCYC 2 cut(s) 270, 297
ErhI CCWWGG 1 cut(s) 40
FaeI CATG 3 cut(s) 72, 103, 157
FaiI YATR 3 cut(s) 70, 101, 155
FatI CATG 3 cut(s) 68, 99, 153
FokI GGATG 1 cut(s) 228
FriOI GRGCYC 2 cut(s) 270, 297
GsuI CTGGAG 2 cut(s) 156, 300
HaeIII GGCC 2 cut(s) 39, 112
HapII CCGG 2 cut(s) 239, 264
Hin1II CATG 3 cut(s) 72, 103, 157
HindIII AAGCTT 2 cut(s) 10, 129
HpaII CCGG 2 cut(s) 239, 264
HphI GGTGA 2 cut(s) 139, 367
Hpy166II GTNNAC 1 cut(s) 147
Hpy188I TCNGA 4 cut(s) 82, 117, 189, 215
Hpy188III TCNNGA 2 cut(s) 135, 163
Hpy8I GTNNAC 1 cut(s) 147
Hpy99I CGWCG 1 cut(s) 35
HpyAV CCTTC 2 cut(s) 135, 402
HpyCH4III ACNGT 2 cut(s) 31, 333
HpyCH4V TGCA 1 cut(s) 153
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 296, 364
HpyF3I CTNAG 2 cut(s) 116, 176
Hsp92II CATG 3 cut(s) 72, 103, 157
Kzo9I GATC 4 cut(s) 184, 195, 210, 348
LguI GCTCTTC 1 cut(s) 275
LmnI GCTCC 1 cut(s) 287
LpnPI CCDG 9 cut(s) 108, 120, 163, 176, 252, 277, 322, 330, 340
LweI GCATC 1 cut(s) 32
MalI GATC 4 cut(s) 186, 197, 212, 350
MboI GATC 4 cut(s) 184, 195, 210, 348
MboII GAAGA 2 cut(s) 69, 262
MflI RGATCY 1 cut(s) 184
MhlI GDGCHC 2 cut(s) 270, 297
MnlI CCTC 8 cut(s) 57, 83, 102, 123, 131, 171, 174, 324
MslI CAYNNNNRTG 1 cut(s) 152
MspI CCGG 2 cut(s) 239, 264
MspR9I CCNGG 1 cut(s) 265
MwoI GCNNNNNNNGC 3 cut(s) 45, 296, 364
NciI CCSGG 1 cut(s) 265
NdeII GATC 4 cut(s) 184, 195, 210, 348
NlaIII CATG 3 cut(s) 72, 103, 157
NlaIV GGNNCC 2 cut(s) 225, 289
NmeAIII GCCGAG 1 cut(s) 316
NspI RCATGY 1 cut(s) 72
PaqCI CACCTGC 1 cut(s) 158
PceI AGGCCT 2 cut(s) 39, 112
PciSI GCTCTTC 1 cut(s) 275
Psp124BI GAGCTC 1 cut(s) 297
PspN4I GGNNCC 2 cut(s) 225, 289
PspPI GGNCC 1 cut(s) 411
PsuI RGATCY 1 cut(s) 184
RsaI GTAC 1 cut(s) 422
RsaNI GTAC 1 cut(s) 421
RseI CAYNNNNRTG 1 cut(s) 152
SacI GAGCTC 1 cut(s) 297
SapI GCTCTTC 1 cut(s) 275
Sau3AI GATC 4 cut(s) 184, 195, 210, 348
Sau96I GGNCC 1 cut(s) 411
ScaI AGTACT 1 cut(s) 422
ScrFI CCNGG 1 cut(s) 265
SduI GDGCHC 2 cut(s) 270, 297
SetI ASST 9 cut(s) 14, 50, 133, 146, 152, 161, 297, 316, 322
SfaNI GCATC 1 cut(s) 32
SinI GGWCC 1 cut(s) 411
SmiMI CAYNNNNRTG 1 cut(s) 152
SseBI AGGCCT 2 cut(s) 39, 112
SsiI CCGC 1 cut(s) 21
SstI GAGCTC 1 cut(s) 297
StuI AGGCCT 2 cut(s) 39, 112
StyD4I CCNGG 1 cut(s) 263
StyI CCWWGG 1 cut(s) 40
TaaI ACNGT 2 cut(s) 31, 333
TaqI TCGA 2 cut(s) 26, 230
TaqII GACCGA 1 cut(s) 428
TatI WGTACW 1 cut(s) 420
TspDTI ATGAA 1 cut(s) 361
VpaK11BI GGWCC 1 cut(s) 411
XceI RCATGY 1 cut(s) 72
ZrmI AGTACT 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.