Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0008735

Physical Location & Seq

Genomic Coordinates Reverse (-)

19118533 .. 19125763

Gene Structure 7231 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1160200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1170 bp

ATGGAGGCTCCGGGAAGAGAAGAGACCAACAAATCTGATGCCCACTTGACATCAGCAGCAGCTTTTGTGGAGGGAGGAATCCAGGAAGCCTGTGATGATGCTTGCAGTATATGCCTGGAGGCCTTCTGTGACAACGATCCTTCTACGTTGACCGGTTGCAAGCATGAATTTCATCTTCAGTGCATTCTTGAGTGGTGTCAGCGAAGCTCCCAATGCCCTATGTGCTGGCAACCCATCAGCTTGAAGGATCCCACTGGCCAGGAACTGTTAGAGGCAGTAGAACAAGAGAGAAGTATAAGGTCGAATCCACCTCGAAATACCACAATATTTCATCATCCAACTCTTGGGGATTTTGAGTTGCAGCATTTACCTATGGGTGTAAATGATGCTGAACTTGAAGAGCGTATAATCCAGCACTTAGCTGCTGCTGCCGCAATGGGTCGGGCACGCCACATTGCCAGAAGGGAAGGACAAAGGAATAGGTCATCAGCTCAAGGTCGTCCACAATACCTGGTCTTCTCTACCAATCCTAATGCACCTCAGGCTCATGCTTCTTCTTCCCCTGATCAGATGGGAGAAGGTGAACCATCTCCTTCAATTGCAGTAACTTTCCCATCGCCTCCTATGAATAGGGAAGAATCTTCTCAAGTAACCACTCCTGTTCATTCGTCACAATCTGGTTCTGTTTCTGCATCAGCATCTGGATCTAGTAATTCGGCTCTGAATCAACAAGGGTCCTCCATGAGCAATAGGAGGTCGCCTAGTCAGTCATCCCCAAGTACCCAAGATAGAGCTGGACCATCAGACTTGCAATCCTTTTCAGAATCTCTGAAATCTCGATTTAATGCAGTATCAACGAGATACAAAGAGTCAATTTCAAAGAGCACAAGAGGTTGGAAGGAGAGACTGTTCTCTCGCAATGCTTCCATGGCAGATCTTGGTTCAGAAGTTAAAAGAGAGGTTAATGCAGGGATCGCAACTGTATCACGCATGATGGAGCGTCTGGAAACAAAAGACGATAACACAATTAATGAGGCTTCTGTGTCAAATAGTTTAGACAGTCCGGTTTCTGAATCAGACAATCGGCGGATATCAGAAGGGGATGGTGAAAATTCTTTAAATGACCACAATAGACCGGCCGCCTGTGCTGCAGGTTCTAGTTCAAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009553 GO:0009555 GO:0009561 GO:0009987 GO:0010498 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0032501 GO:0032502 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044464 GO:0048229 GO:0048856 GO:0050789 GO:0050794 GO:0051603 GO:0051726 GO:0055046 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_11 zf-RING_2

Protein Analysis

390

Amino Acids

42.1

Weight (kDa)

5.38

Isoelectric Point (pI)

67.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	29 - 75	1e-06	RING-H2 zinc finger domain
zf-RING_2	PF13639	34 - 75	2.1e-09	Ring finger domain
zf-RING_11	PF17123	35 - 62	4e-07	RING-like zinc finger
zf-C3HC4_2	PF13923	35 - 75	3e-06	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	35 - 75	7.9e-06	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008735)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22000 AT5G22000 AT5G22000 AT5G22000
fragaria_vesca	FvH4_3g26760 FvH4_3g26760
malus_domestica	MD03G1160200.v1.1 MD11G1176400.v1.1
prunus_persica	Prupe.6G148500_v2.0.a1 Prupe.6G148500_v2.0.a1 Prupe.6G148500_v2.0.a1
pyrus_communis	pycom03g11270 pycom11g15060
rosa_chinensis	RchiOBHm_Chr5g0049191
rosa_laevigata	RLG00000034598
rosa_multiflora	Rmu_sc0001440.1_g000001
rosa_roxburghii	Rroxscaffold_1G00032540
rosa_rugosa	Rorug05G0245500 Rorug05G0245600.1
rosa_samantha	Rh5AG323500 Rh5BG333900 Rh5DG345600
rosa_wichuraiana	Rw5G030550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1142
AccB7I	CCANNNNNTGG	1 cut(s)	344
AciI	CCGC	3 cut(s)	432, 1087, 1140
AclWI	GGATC	5 cut(s)	131, 242, 255, 712, 980
AcoI	YGGCCR	2 cut(s)	256, 1137
AcsI	RAATTY	2 cut(s)	167, 1111
AcuI	CTGAAG	1 cut(s)	161
AfaI	GTAC	1 cut(s)	781
AfiI	CCNNNNNNNGG	1 cut(s)	344
AgeI	ACCGGT	1 cut(s)	152
AgsI	TTSAA	5 cut(s)	244, 398, 597, 879, 1164
AjnI	CCWGG	4 cut(s)	81, 114, 258, 510
AloI	GAACNNNNNNTCC	2 cut(s)	893, 925
AluBI	AGCT	6 cut(s)	62, 207, 240, 422, 491, 794
AluI	AGCT	6 cut(s)	62, 207, 240, 422, 491, 794
Alw21I	GWGCWC	1 cut(s)	887
Alw26I	GTCTC	2 cut(s)	17, 898
AlwI	GGATC	5 cut(s)	131, 242, 255, 712, 980
AlwNI	CAGNNNCTG	2 cut(s)	265, 701
AoxI	GGCC	3 cut(s)	120, 256, 1137
ApeKI	GCWGC	7 cut(s)	56, 59, 361, 422, 425, 428, 1148
ApoI	RAATTY	2 cut(s)	167, 1111
AseI	ATTAAT	1 cut(s)	1029
AsiGI	ACCGGT	1 cut(s)	152
AspS9I	GGNCC	2 cut(s)	735, 797
AsuC2I	CCSGG	1 cut(s)	12
AsuHPI	GGTGA	2 cut(s)	593, 1118
AvaII	GGWCC	2 cut(s)	735, 797
AxyI	CCTNAGG	1 cut(s)	540
BaeGI	GKGCMC	1 cut(s)	448
BalI	TGGCCA	1 cut(s)	258
BamHI	GGATCC	1 cut(s)	247
BbsI	GAAGAC	1 cut(s)	508
Bbv12I	GWGCWC	1 cut(s)	887
BbvI	GCAGC	7 cut(s)	68, 71, 373, 409, 412, 415, 1135
BccI	CCATC	7 cut(s)	242, 565, 595, 622, 808, 988, 1097
BciT130I	CCWGG	4 cut(s)	83, 116, 260, 512
BclI	TGATCA	1 cut(s)	565
BcnI	CCSGG	1 cut(s)	12
BcoDI	GTCTC	2 cut(s)	17, 898
BfaI	CTAG	3 cut(s)	708, 762, 1158
BfmI	CTRYAG	1 cut(s)	1149
BfuAI	ACCTGC	1 cut(s)	1142
BglII	AGATCT	1 cut(s)	934
BisI	GCNGC	9 cut(s)	57, 60, 362, 423, 426, 429, 432, 1140, 1149
BlsI	GCNGC	9 cut(s)	58, 61, 363, 424, 427, 430, 433, 1141, 1150
Bme1390I	CCNGG	5 cut(s)	12, 83, 116, 260, 512
Bme18I	GGWCC	2 cut(s)	735, 797
BmgT120I	GGNCC	2 cut(s)	735, 797
BmiI	GGNNCC	3 cut(s)	9, 249, 736
BmrFI	CCNGG	5 cut(s)	12, 83, 116, 260, 512
BmsI	GCATC	5 cut(s)	28, 88, 376, 701, 707
BpiI	GAAGAC	1 cut(s)	508
BpmI	CTGGAG	1 cut(s)	137
BpuEI	CTTGAG	3 cut(s)	209, 477, 630
BpuMI	CCSGG	1 cut(s)	12
BsaI	GGTCTC	1 cut(s)	17
BsaJI	CCNNGG	1 cut(s)	927
BsaWI	WCCGGW	2 cut(s)	152, 1063
BsaXI	ACNNNNNCTCC	2 cut(s)	893, 923
Bsc4I	CCNNNNNNNGG	1 cut(s)	344
Bse118I	RCCGGY	2 cut(s)	152, 1135
Bse1I	ACTGG	1 cut(s)	259
Bse21I	CCTNAGG	1 cut(s)	540
Bse3DI	GCAATG	3 cut(s)	441, 453, 925
BseBI	CCWGG	4 cut(s)	83, 116, 260, 512
BseDI	CCNNGG	1 cut(s)	927
BseGI	GGATG	3 cut(s)	334, 770, 1108
BseLI	CCNNNNNNNGG	1 cut(s)	344
BseMI	GCAATG	3 cut(s)	441, 453, 925
BseMII	CTCAG	1 cut(s)	554
BseNI	ACTGG	1 cut(s)	259
BseSI	GKGCMC	1 cut(s)	448
BseX3I	CGGCCG	1 cut(s)	1137
BseXI	GCAGC	7 cut(s)	68, 71, 373, 409, 412, 415, 1135
Bsh1285I	CGRYCG	1 cut(s)	1140
BshFI	GGCC	3 cut(s)	122, 258, 1139
BshTI	ACCGGT	1 cut(s)	152
BsiEI	CGRYCG	1 cut(s)	1140
BsiHKAI	GWGCWC	1 cut(s)	887
BsiSI	CCGG	4 cut(s)	11, 153, 1064, 1136
BslI	CCNNNNNNNGG	1 cut(s)	344
BsmAI	GTCTC	2 cut(s)	17, 898
BsmI	GAATGC	1 cut(s)	183
BsnI	GGCC	3 cut(s)	122, 258, 1139
Bso31I	GGTCTC	1 cut(s)	17
Bsp1286I	GDGCHC	2 cut(s)	448, 887
Bsp143I	GATC	6 cut(s)	136, 247, 565, 704, 934, 972
Bsp19I	CCATGG	1 cut(s)	927
BspACI	CCGC	3 cut(s)	432, 1087, 1140
BspANI	GGCC	3 cut(s)	122, 258, 1139
BspCNI	CTCAG	1 cut(s)	553
BspLI	GGNNCC	3 cut(s)	9, 249, 736
BspMAI	CTGCAG	1 cut(s)	1153
BspMI	ACCTGC	1 cut(s)	1142
BspPI	GGATC	5 cut(s)	131, 242, 255, 712, 980
BspQI	GCTCTTC	1 cut(s)	393
BspTNI	GGTCTC	1 cut(s)	17
BsrDI	GCAATG	3 cut(s)	441, 453, 925
BsrFI	RCCGGY	2 cut(s)	152, 1135
BsrI	ACTGG	1 cut(s)	259
BssAI	RCCGGY	2 cut(s)	152, 1135
BssECI	CCNNGG	1 cut(s)	927
BssMI	GATC	6 cut(s)	136, 247, 565, 704, 934, 972
BssT1I	CCWWGG	1 cut(s)	927
Bst2UI	CCWGG	4 cut(s)	83, 116, 260, 512
Bst4CI	ACNGT	4 cut(s)	267, 909, 982, 1061
Bst6I	CTCTTC	3 cut(s)	10, 15, 393
BstAPI	GCANNNNNTGC	1 cut(s)	111
BstC8I	GCNNGC	4 cut(s)	103, 161, 227, 448
BstDEI	CTNAG	2 cut(s)	418, 540
BstDSI	CCRYGG	1 cut(s)	927
BstF5I	GGATG	3 cut(s)	334, 770, 1108
BstKTI	GATC	6 cut(s)	139, 250, 568, 707, 937, 975
BstMAI	GTCTC	2 cut(s)	17, 898
BstMBI	GATC	6 cut(s)	136, 247, 565, 704, 934, 972
BstMCI	CGRYCG	1 cut(s)	1140
BstNI	CCWGG	4 cut(s)	83, 116, 260, 512
BstSCI	CCNGG	5 cut(s)	10, 81, 114, 258, 510
BstSFI	CTRYAG	1 cut(s)	1149
BstSLI	GKGCMC	1 cut(s)	448
BstV1I	GCAGC	7 cut(s)	68, 71, 373, 409, 412, 415, 1135
BstV2I	GAAGAC	1 cut(s)	508
BstX2I	RGATCY	3 cut(s)	247, 704, 934
BstYI	RGATCY	3 cut(s)	247, 704, 934
BstZI	CGGCCG	1 cut(s)	1137
Bsu36I	CCTNAGG	1 cut(s)	540
BsuRI	GGCC	3 cut(s)	122, 258, 1139
BtgI	CCRYGG	1 cut(s)	927
BtgZI	GCGATG	1 cut(s)	600
BtsCI	GGATG	3 cut(s)	334, 770, 1108
BtsIMutI	CAGTG	2 cut(s)	185, 252
BveI	ACCTGC	1 cut(s)	1142
Cac8I	GCNNGC	4 cut(s)	103, 161, 227, 448
CaiI	CAGNNNCTG	2 cut(s)	265, 701
Cfr10I	RCCGGY	2 cut(s)	152, 1135
Cfr13I	GGNCC	2 cut(s)	735, 797
CseI	GACGC	1 cut(s)	989
CsiI	ACCWGGT	1 cut(s)	510
Csp6I	GTAC	1 cut(s)	780
CspAI	ACCGGT	1 cut(s)	152
CviAII	CATG	5 cut(s)	164, 548, 742, 928, 991
CviQI	GTAC	1 cut(s)	780
DdeI	CTNAG	2 cut(s)	418, 540
DpnI	GATC	6 cut(s)	138, 249, 567, 706, 936, 974
DpnII	GATC	6 cut(s)	136, 247, 565, 704, 934, 972
DraI	TTTAAA	1 cut(s)	1119
EaeI	YGGCCR	2 cut(s)	256, 1137
EagI	CGGCCG	1 cut(s)	1137
Eam1104I	CTCTTC	3 cut(s)	10, 15, 393
EarI	CTCTTC	3 cut(s)	10, 15, 393
EciI	GGCGGA	1 cut(s)	1102
EclXI	CGGCCG	1 cut(s)	1137
Eco130I	CCWWGG	1 cut(s)	927
Eco147I	AGGCCT	1 cut(s)	122
Eco31I	GGTCTC	1 cut(s)	17
Eco32I	GATATC	1 cut(s)	1092
Eco47I	GGWCC	2 cut(s)	735, 797
Eco52I	CGGCCG	1 cut(s)	1137
Eco57I	CTGAAG	1 cut(s)	161
Eco81I	CCTNAGG	1 cut(s)	540
EcoO109I	RGGNCCY	1 cut(s)	735
EcoRII	CCWGG	4 cut(s)	81, 114, 258, 510
EcoRV	GATATC	1 cut(s)	1092
EcoT14I	CCWWGG	1 cut(s)	927
ErhI	CCWWGG	1 cut(s)	927
FaeI	CATG	5 cut(s)	167, 551, 745, 931, 994
FatI	CATG	5 cut(s)	163, 547, 741, 927, 990
FbaI	TGATCA	1 cut(s)	565
Fnu4HI	GCNGC	9 cut(s)	57, 60, 362, 423, 426, 429, 432, 1140, 1149
FokI	GGATG	3 cut(s)	321, 757, 1115
Fsp4HI	GCNGC	9 cut(s)	57, 60, 362, 423, 426, 429, 432, 1140, 1149
FspBI	CTAG	3 cut(s)	708, 762, 1158
GluI	GCNGC	9 cut(s)	57, 60, 362, 423, 426, 429, 432, 1140, 1149
GsuI	CTGGAG	1 cut(s)	137
HaeIII	GGCC	3 cut(s)	122, 258, 1139
HapII	CCGG	4 cut(s)	11, 153, 1064, 1136
HgaI	GACGC	1 cut(s)	989
Hin1II	CATG	5 cut(s)	167, 551, 745, 931, 994
HincII	GTYRAC	1 cut(s)	150
HindII	GTYRAC	1 cut(s)	150
HinfI	GANTC	7 cut(s)	78, 304, 638, 724, 824, 869, 1073
HpaII	CCGG	4 cut(s)	11, 153, 1064, 1136
HphI	GGTGA	2 cut(s)	593, 1118
Hpy166II	GTNNAC	3 cut(s)	150, 503, 584
Hpy188III	TCNNGA	4 cut(s)	188, 702, 837, 1004
Hpy8I	GTNNAC	3 cut(s)	150, 503, 584
HpyAV	CCTTC	9 cut(s)	133, 150, 238, 456, 461, 572, 603, 892, 1091
HpyCH4III	ACNGT	4 cut(s)	267, 909, 982, 1061
HpyCH4IV	ACGT	1 cut(s)	146
HpyF3I	CTNAG	2 cut(s)	418, 540
HpySE526I	ACGT	1 cut(s)	146
Hsp92II	CATG	5 cut(s)	167, 551, 745, 931, 994
Ksp22I	TGATCA	1 cut(s)	565
Kzo9I	GATC	6 cut(s)	136, 247, 565, 704, 934, 972
LguI	GCTCTTC	1 cut(s)	393
LmnI	GCTCC	3 cut(s)	13, 212, 997
Lsp1109I	GCAGC	7 cut(s)	68, 71, 373, 409, 412, 415, 1135
LweI	GCATC	5 cut(s)	28, 88, 376, 701, 707
MabI	ACCWGGT	1 cut(s)	510
MaeI	CTAG	3 cut(s)	708, 762, 1158
MaeII	ACGT	1 cut(s)	146
MaeIII	GTNAC	4 cut(s)	128, 604, 649, 669
MalI	GATC	6 cut(s)	138, 249, 567, 706, 936, 974
MboI	GATC	6 cut(s)	136, 247, 565, 704, 934, 972
MboII	GAAGA	9 cut(s)	27, 32, 167, 410, 508, 546, 549, 633, 647
MfeI	CAATTG	1 cut(s)	597
MflI	RGATCY	3 cut(s)	247, 704, 934
MhlI	GDGCHC	2 cut(s)	448, 887
MlsI	TGGCCA	1 cut(s)	258
MluCI	AATT	7 cut(s)	167, 597, 712, 873, 1026, 1111, 1165
MluNI	TGGCCA	1 cut(s)	258
MlyI	GAGTC	1 cut(s)	878
MmeI	TCCRAC	2 cut(s)	362, 875
Mox20I	TGGCCA	1 cut(s)	258
MscI	TGGCCA	1 cut(s)	258
MseI	TTAA	5 cut(s)	843, 951, 963, 1029, 1118
Msp20I	TGGCCA	1 cut(s)	258
MspI	CCGG	4 cut(s)	11, 153, 1064, 1136
MspR9I	CCNGG	5 cut(s)	12, 83, 116, 260, 512
MunI	CAATTG	1 cut(s)	597
Mva1269I	GAATGC	1 cut(s)	183
MvaI	CCWGG	4 cut(s)	83, 116, 260, 512
NciI	CCSGG	1 cut(s)	12
NcoI	CCATGG	1 cut(s)	927
NdeII	GATC	6 cut(s)	136, 247, 565, 704, 934, 972
NlaIII	CATG	5 cut(s)	167, 551, 745, 931, 994
NlaIV	GGNNCC	3 cut(s)	9, 249, 736
NmuCI	GTSAC	2 cut(s)	128, 669
PceI	AGGCCT	1 cut(s)	122
PciSI	GCTCTTC	1 cut(s)	393
PctI	GAATGC	1 cut(s)	183
PfeI	GAWTC	6 cut(s)	78, 304, 638, 724, 824, 1073
PflMI	CCANNNNNTGG	1 cut(s)	344
PfoI	TCCNGGA	2 cut(s)	10, 81
PinAI	ACCGGT	1 cut(s)	152
PkrI	GCNGC	9 cut(s)	58, 61, 363, 424, 427, 430, 433, 1141, 1150
PleI	GAGTC	1 cut(s)	877
PpsI	GAGTC	1 cut(s)	877
PpuMI	RGGWCCY	1 cut(s)	735
PshBI	ATTAAT	1 cut(s)	1029
Psp5II	RGGWCCY	1 cut(s)	735
Psp6I	CCWGG	4 cut(s)	81, 114, 258, 510
PspGI	CCWGG	4 cut(s)	81, 114, 258, 510
PspN4I	GGNNCC	3 cut(s)	9, 249, 736
PspPI	GGNCC	2 cut(s)	735, 797
PspPPI	RGGWCCY	1 cut(s)	735
PstI	CTGCAG	1 cut(s)	1153
PstNI	CAGNNNCTG	2 cut(s)	265, 701
PsuI	RGATCY	3 cut(s)	247, 704, 934
RsaI	GTAC	1 cut(s)	781
RsaNI	GTAC	1 cut(s)	780
SapI	GCTCTTC	1 cut(s)	393
SaqAI	TTAA	5 cut(s)	843, 951, 963, 1029, 1118
SatI	GCNGC	9 cut(s)	57, 60, 362, 423, 426, 429, 432, 1140, 1149
Sau3AI	GATC	6 cut(s)	136, 247, 565, 704, 934, 972
Sau96I	GGNCC	2 cut(s)	735, 797
SchI	GAGTC	1 cut(s)	878
ScrFI	CCNGG	5 cut(s)	12, 83, 116, 260, 512
SduI	GDGCHC	2 cut(s)	448, 887
SexAI	ACCWGGT	1 cut(s)	510
SfaNI	GCATC	5 cut(s)	28, 88, 376, 701, 707
SfcI	CTRYAG	1 cut(s)	1149
SinI	GGWCC	2 cut(s)	735, 797
SmlI	CTYRAG	3 cut(s)	188, 492, 645
SmoI	CTYRAG	3 cut(s)	188, 492, 645
Sse9I	AATT	7 cut(s)	167, 597, 712, 873, 1026, 1111, 1165
SseBI	AGGCCT	1 cut(s)	122
SsiI	CCGC	3 cut(s)	432, 1087, 1140
SspI	AATATT	1 cut(s)	327
SspMI	CTAG	3 cut(s)	708, 762, 1158
StuI	AGGCCT	1 cut(s)	122
StyD4I	CCNGG	5 cut(s)	10, 81, 114, 258, 510
StyI	CCWWGG	1 cut(s)	927
TaaI	ACNGT	4 cut(s)	267, 909, 982, 1061
TaiI	ACGT	1 cut(s)	149
TaqI	TCGA	3 cut(s)	302, 313, 838
TasI	AATT	7 cut(s)	167, 597, 712, 873, 1026, 1111, 1165
TauI	GCSGC	2 cut(s)	434, 1142
TfiI	GAWTC	6 cut(s)	78, 304, 638, 724, 824, 1073
Tru1I	TTAA	5 cut(s)	843, 951, 963, 1029, 1118
Tru9I	TTAA	5 cut(s)	843, 951, 963, 1029, 1118
TscAI	CASTG	2 cut(s)	185, 259
TseFI	GTSAC	2 cut(s)	128, 669
TseI	GCWGC	7 cut(s)	56, 59, 361, 422, 425, 428, 1148
Tsp45I	GTSAC	2 cut(s)	128, 669
TspDTI	ATGAA	5 cut(s)	161, 180, 320, 641, 653
TspRI	CASTG	2 cut(s)	185, 259
Van91I	CCANNNNNTGG	1 cut(s)	344
VpaK11BI	GGWCC	2 cut(s)	735, 797
VspI	ATTAAT	1 cut(s)	1029
XapI	RAATTY	2 cut(s)	167, 1111
XcmI	CCANNNNNNNNNTGG	1 cut(s)	791
XspI	CTAG	3 cut(s)	708, 762, 1158

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.