Rw5G030550

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008735
Physical Location & Seq
Reverse (-)
48533009 .. 48539826
6818 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G030550.1

Sequence Viewer

Length: 1176 bp
ATGGAGGTTCCTCCTGGAATGGAAGAGAGCGGCAAGTCTGAGGCCCACTTGACCTCCCCAGCGGCTTTTGTGGAGGGTGGAATACAGGAGGCATGTGATGATGCTTGCAGTATATGCCTTGAGGCTTTCTGTGATAGTGATCCTTCTACGTTGACCGGTTGCAAGCATGAATTTCATCTTCAGTGCATTCTTGAATGGTGTCAGAGAAGCTCCCAATGCCCTATGTGCTGGCAGCCCATCAGCTTAAAAGACCCCACCAGCCAAGAACTGTTTGAGGGTGTAGAACGGGAGAGAAGTTATAGGTCAAACCCACCTCGAAGTACTGCAATATTTCATCATCCAACTCTTGGCGATTTTGAACTACAACATTTACCTGTGGGTGTGAATGATGCTGAACTTGAAGAGCGTATAATTCAGCACTTAGCTGCTGCTGCTGCAATGGGTCGGGCACGCCATATTGCCAGAAGGGAGGGCCAGAGAAATAGGTCATCAGGTCAAGGTCGACCACAATTCCTGGTCTTCTCTACTCATCCTAATGCACCTCCTACCACAGCTTCTTCCTCCCCAGATCAGATGGGAGCAGGTGAACAAGCTCCTTCCGTTGCAGTAACTTTTCCGTCACCTCCTATGAATGCTGGAGAAGAAACTTCTCGAGTGACTACTTCAGGACCTTCTTCTCAATCTGGTCCTGTTTCTGCCTCAGCATCTGGATCTAGTGTCCCTCCATCCAATCAACATGGGTCTTCCATAAGTACTAGGAGGTCTCCTAATCAGTCATCCCCAAGTAGCCAAGATAGAGCTGGGCCATCAGATTTACAGTCATTTTCAGAAAATCTGAAGGCTCGATTCAGTGCAGTGTCAATGAAATACAAAGAGTCGATTTCAAAGAGCACAAGAGGTTGGAAGGAGAGATTGTTCTCTCGCAACAGTTCTGTTTCCGATCTTGGTCCCGAAGTTAAAAGAGAGGTTGATGAAGGGATTGCAACAGTATCACGCATGATGGAGCGTCTGGAAACAAGAGAAAATAATAGAATCAATGGTGCTTCTGGATCAGATGGTATAAACAGTTCAGTTCCCGAATCAGAAAATAGGCGAATATCAGAAAATGGTGGTCAAAATTCTCTGAGTGAGCAGAATAAGCCAGCTACATGTGCTGCTGGCTCTAGTTCAAATTAG

Protein Analysis

391

Amino Acids

41.99

Weight (kDa)

5.66

Isoelectric Point (pI)

67.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 30 - 76 1.1e-06 RING-H2 zinc finger domain
zf-RING_2 PF13639 35 - 76 2.4e-09 Ring finger domain
zf-RING_11 PF17123 36 - 63 6e-07 RING-like zinc finger
zf-C3HC4_2 PF13923 36 - 76 2.6e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 36 - 76 8.7e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 572
Acc36I ACCTGC 1 cut(s) 572
AccB7I CCANNNNNTGG 1 cut(s) 347
AccBSI CCGCTC 1 cut(s) 30
AccI GTMKAC 1 cut(s) 502
AciI CCGC 2 cut(s) 30, 62
AclWI GGATC 3 cut(s) 134, 718, 1057
AcsI RAATTY 2 cut(s) 170, 1117
AcuI CTGAAG 3 cut(s) 164, 648, 857
AfaI GTAC 2 cut(s) 322, 754
AfiI CCNNNNNNNGG 1 cut(s) 347
AflIII ACRYGT 1 cut(s) 1148
AgeI ACCGGT 1 cut(s) 155
AgsI TTSAA 5 cut(s) 194, 359, 401, 885, 1170
AjnI CCWGG 2 cut(s) 13, 513
AloI GAACNNNNNNTCC 2 cut(s) 899, 931
AluBI AGCT 7 cut(s) 210, 243, 425, 554, 593, 800, 1145
AluI AGCT 7 cut(s) 210, 243, 425, 554, 593, 800, 1145
Alw21I GWGCWC 1 cut(s) 893
Alw26I GTCTC 1 cut(s) 768
AlwI GGATC 3 cut(s) 134, 718, 1057
AlwNI CAGNNNCTG 1 cut(s) 707
Ama87I CYCGRG 1 cut(s) 651
AoxI GGCC 3 cut(s) 42, 472, 803
ApeKI GCWGC 6 cut(s) 232, 425, 428, 431, 434, 1154
ApoI RAATTY 2 cut(s) 170, 1117
AsiGI ACCGGT 1 cut(s) 155
AspS9I GGNCC 6 cut(s) 43, 472, 668, 686, 803, 947
AsuHPI GGTGA 2 cut(s) 596, 612
AvaI CYCGRG 1 cut(s) 651
AvaII GGWCC 3 cut(s) 668, 686, 947
BaeGI GKGCMC 1 cut(s) 451
BbsI GAAGAC 2 cut(s) 511, 735
Bbv12I GWGCWC 1 cut(s) 893
BbvCI CCTCAGC 1 cut(s) 700
BbvI GCAGC 6 cut(s) 244, 412, 415, 418, 421, 1141
BccI CCATC 6 cut(s) 245, 568, 733, 814, 994, 1049
BciT130I CCWGG 2 cut(s) 15, 515
BcoDI GTCTC 1 cut(s) 768
BfaI CTAG 3 cut(s) 714, 756, 1164
BfuAI ACCTGC 1 cut(s) 572
BisI GCNGC 8 cut(s) 31, 63, 233, 426, 429, 432, 435, 1155
BlsI GCNGC 8 cut(s) 32, 64, 234, 427, 430, 433, 436, 1156
BmcAI AGTACT 2 cut(s) 322, 754
Bme1390I CCNGG 2 cut(s) 15, 515
Bme18I GGWCC 3 cut(s) 668, 686, 947
BmeT110I CYCGRG 1 cut(s) 651
BmgT120I GGNCC 6 cut(s) 43, 472, 668, 686, 803, 947
BmiI GGNNCC 2 cut(s) 9, 949
BmrFI CCNGG 2 cut(s) 15, 515
BmsI GCATC 3 cut(s) 91, 379, 713
BpiI GAAGAC 2 cut(s) 511, 735
BpmI CTGGAG 1 cut(s) 657
Bpu10I CCTNAGC 1 cut(s) 700
BpuEI CTTGAG 1 cut(s) 140
BsaBI GATNNNNATC 1 cut(s) 138
BsaI GGTCTC 1 cut(s) 768
BsaWI WCCGGW 1 cut(s) 155
BsaXI ACNNNNNCTCC 4 cut(s) 38, 68, 899, 929
Bsc4I CCNNNNNNNGG 1 cut(s) 347
Bse118I RCCGGY 1 cut(s) 155
Bse3DI GCAATG 1 cut(s) 444
Bse8I GATNNNNATC 1 cut(s) 138
BseBI CCWGG 2 cut(s) 15, 515
BseGI GGATG 4 cut(s) 337, 529, 725, 776
BseJI GATNNNNATC 1 cut(s) 138
BseLI CCNNNNNNNGG 1 cut(s) 347
BseMI GCAATG 1 cut(s) 444
BseMII CTCAG 3 cut(s) 30, 714, 1115
BseSI GKGCMC 1 cut(s) 451
BseXI GCAGC 6 cut(s) 244, 412, 415, 418, 421, 1141
BseYI CCCAGC 2 cut(s) 58, 800
BsgI GTGCAG 1 cut(s) 873
BshFI GGCC 3 cut(s) 44, 474, 805
BshTI ACCGGT 1 cut(s) 155
BsiHKAI GWGCWC 1 cut(s) 893
BsiHKCI CYCGRG 1 cut(s) 651
BsiSI CCGG 1 cut(s) 156
BslFI GGGAC 2 cut(s) 704, 933
BslI CCNNNNNNNGG 1 cut(s) 347
BsmAI GTCTC 1 cut(s) 768
BsmFI GGGAC 2 cut(s) 704, 933
BsmI GAATGC 2 cut(s) 186, 637
BsnI GGCC 3 cut(s) 44, 474, 805
Bso31I GGTCTC 1 cut(s) 768
BsoBI CYCGRG 1 cut(s) 651
Bsp1286I GDGCHC 2 cut(s) 451, 893
Bsp143I GATC 5 cut(s) 139, 568, 710, 940, 1049
BspACI CCGC 2 cut(s) 30, 62
BspANI GGCC 3 cut(s) 44, 474, 805
BspCNI CTCAG 3 cut(s) 31, 713, 1116
BspLI GGNNCC 2 cut(s) 9, 949
BspMI ACCTGC 1 cut(s) 572
BspPI GGATC 3 cut(s) 134, 718, 1057
BspQI GCTCTTC 1 cut(s) 396
BspTNI GGTCTC 1 cut(s) 768
BsrBI CCGCTC 1 cut(s) 30
BsrDI GCAATG 1 cut(s) 444
BsrFI RCCGGY 1 cut(s) 155
BssAI RCCGGY 1 cut(s) 155
BssMI GATC 5 cut(s) 139, 568, 710, 940, 1049
Bst2UI CCWGG 2 cut(s) 15, 515
Bst4CI ACNGT 5 cut(s) 270, 819, 929, 988, 1067
Bst6I CTCTTC 2 cut(s) 18, 396
BstAPI GCANNNNNTGC 1 cut(s) 114
BstC8I GCNNGC 6 cut(s) 106, 164, 230, 451, 1143, 1159
BstDEI CTNAG 4 cut(s) 39, 421, 700, 1124
BstF5I GGATG 4 cut(s) 337, 529, 725, 776
BstKTI GATC 5 cut(s) 142, 571, 713, 943, 1052
BstMAI GTCTC 1 cut(s) 768
BstMBI GATC 5 cut(s) 139, 568, 710, 940, 1049
BstMWI GCNNNNNNNGC 7 cut(s) 114, 216, 225, 431, 434, 1138, 1151
BstNI CCWGG 2 cut(s) 15, 515
BstNSI RCATGY 2 cut(s) 96, 1152
BstSCI CCNGG 2 cut(s) 13, 513
BstSLI GKGCMC 1 cut(s) 451
BstV1I GCAGC 6 cut(s) 244, 412, 415, 418, 421, 1141
BstV2I GAAGAC 2 cut(s) 511, 735
BstX2I RGATCY 1 cut(s) 710
BstYI RGATCY 1 cut(s) 710
BsuRI GGCC 3 cut(s) 44, 474, 805
BtsCI GGATG 4 cut(s) 337, 529, 725, 776
BtsI GCAGTG 1 cut(s) 861
BtsIMutI CAGTG 3 cut(s) 188, 856, 861
BveI ACCTGC 1 cut(s) 572
Cac8I GCNNGC 6 cut(s) 106, 164, 230, 451, 1143, 1159
CaiI CAGNNNCTG 1 cut(s) 707
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 6 cut(s) 43, 472, 668, 686, 803, 947
CseI GACGC 1 cut(s) 995
Csp6I GTAC 2 cut(s) 321, 753
CspAI ACCGGT 1 cut(s) 155
CviAII CATG 5 cut(s) 93, 167, 737, 997, 1149
CviQI GTAC 2 cut(s) 321, 753
DdeI CTNAG 4 cut(s) 39, 421, 700, 1124
DpnI GATC 5 cut(s) 141, 570, 712, 942, 1051
DpnII GATC 5 cut(s) 139, 568, 710, 940, 1049
Eam1104I CTCTTC 2 cut(s) 18, 396
EarI CTCTTC 2 cut(s) 18, 396
Eco31I GGTCTC 1 cut(s) 768
Eco47I GGWCC 3 cut(s) 668, 686, 947
Eco57I CTGAAG 3 cut(s) 164, 648, 857
Eco88I CYCGRG 1 cut(s) 651
EcoO109I RGGNCCY 1 cut(s) 668
EcoRII CCWGG 2 cut(s) 13, 513
FaeI CATG 5 cut(s) 96, 170, 740, 1000, 1152
FaqI GGGAC 2 cut(s) 704, 933
FatI CATG 5 cut(s) 92, 166, 736, 996, 1148
FblI GTMKAC 1 cut(s) 502
Fnu4HI GCNGC 8 cut(s) 31, 63, 233, 426, 429, 432, 435, 1155
FokI GGATG 4 cut(s) 324, 516, 712, 763
Fsp4HI GCNGC 8 cut(s) 31, 63, 233, 426, 429, 432, 435, 1155
FspBI CTAG 3 cut(s) 714, 756, 1164
GluI GCNGC 8 cut(s) 31, 63, 233, 426, 429, 432, 435, 1155
GsaI CCCAGC 2 cut(s) 62, 804
GsuI CTGGAG 1 cut(s) 657
HaeIII GGCC 3 cut(s) 44, 474, 805
HapII CCGG 1 cut(s) 156
HgaI GACGC 1 cut(s) 995
Hin1II CATG 5 cut(s) 96, 170, 740, 1000, 1152
HincII GTYRAC 2 cut(s) 153, 503
HindII GTYRAC 2 cut(s) 153, 503
HinfI GANTC 4 cut(s) 846, 875, 1032, 1079
HpaII CCGG 1 cut(s) 156
HphI GGTGA 2 cut(s) 596, 612
Hpy166II GTNNAC 3 cut(s) 153, 503, 587
Hpy188III TCNNGA 8 cut(s) 191, 651, 666, 708, 950, 1010, 1047, 1076
Hpy8I GTNNAC 3 cut(s) 153, 503, 587
HpyAV CCTTC 7 cut(s) 153, 459, 606, 681, 832, 898, 968
HpyCH4III ACNGT 5 cut(s) 270, 819, 929, 988, 1067
HpyCH4IV ACGT 1 cut(s) 149
HpyCH4V TGCA 9 cut(s) 108, 162, 186, 326, 437, 539, 605, 854, 983
HpyF10VI GCNNNNNNNGC 7 cut(s) 114, 216, 225, 431, 434, 1138, 1151
HpyF3I CTNAG 4 cut(s) 39, 421, 700, 1124
HpySE526I ACGT 1 cut(s) 149
Hsp92II CATG 5 cut(s) 96, 170, 740, 1000, 1152
Kzo9I GATC 5 cut(s) 139, 568, 710, 940, 1049
LguI GCTCTTC 1 cut(s) 396
LmnI GCTCC 4 cut(s) 215, 578, 598, 1003
Lsp1109I GCAGC 6 cut(s) 244, 412, 415, 418, 421, 1141
LweI GCATC 3 cut(s) 91, 379, 713
MaeI CTAG 3 cut(s) 714, 756, 1164
MaeII ACGT 1 cut(s) 149
MaeIII GTNAC 3 cut(s) 607, 618, 655
MalI GATC 5 cut(s) 141, 570, 712, 942, 1051
MbiI CCGCTC 1 cut(s) 30
MboI GATC 5 cut(s) 139, 568, 710, 940, 1049
MboII GAAGA 8 cut(s) 35, 170, 413, 511, 549, 653, 666, 735
MflI RGATCY 1 cut(s) 710
MhlI GDGCHC 2 cut(s) 451, 893
MluCI AATT 5 cut(s) 170, 411, 509, 1117, 1171
MlyI GAGTC 1 cut(s) 884
MmeI TCCRAC 2 cut(s) 365, 881
MseI TTAA 2 cut(s) 245, 957
MslI CAYNNNNRTG 1 cut(s) 534
MspA1I CMGCKG 1 cut(s) 62
MspI CCGG 1 cut(s) 156
MspR9I CCNGG 2 cut(s) 15, 515
Mva1269I GAATGC 2 cut(s) 186, 637
MvaI CCWGG 2 cut(s) 15, 515
MwoI GCNNNNNNNGC 7 cut(s) 114, 216, 225, 431, 434, 1138, 1151
NdeII GATC 5 cut(s) 139, 568, 710, 940, 1049
NlaIII CATG 5 cut(s) 96, 170, 740, 1000, 1152
NlaIV GGNNCC 2 cut(s) 9, 949
NmuCI GTSAC 2 cut(s) 618, 655
NspI RCATGY 2 cut(s) 96, 1152
PaeR7I CTCGAG 1 cut(s) 651
PaqCI CACCTGC 1 cut(s) 572
PciI ACATGT 1 cut(s) 1148
PciSI GCTCTTC 1 cut(s) 396
PctI GAATGC 2 cut(s) 186, 637
PfeI GAWTC 3 cut(s) 846, 1032, 1079
PflMI CCANNNNNTGG 1 cut(s) 347
PfoI TCCNGGA 1 cut(s) 13
PinAI ACCGGT 1 cut(s) 155
PkrI GCNGC 8 cut(s) 32, 64, 234, 427, 430, 433, 436, 1156
PleI GAGTC 1 cut(s) 883
PpsI GAGTC 1 cut(s) 883
PpuMI RGGWCCY 1 cut(s) 668
PscI ACATGT 1 cut(s) 1148
Psp5II RGGWCCY 1 cut(s) 668
Psp6I CCWGG 2 cut(s) 13, 513
PspFI CCCAGC 2 cut(s) 58, 800
PspGI CCWGG 2 cut(s) 13, 513
PspN4I GGNNCC 2 cut(s) 9, 949
PspPI GGNCC 6 cut(s) 43, 472, 668, 686, 803, 947
PspPPI RGGWCCY 1 cut(s) 668
PsrI GAACNNNNNNTAC 2 cut(s) 1051, 1083
PstNI CAGNNNCTG 1 cut(s) 707
PsuI RGATCY 1 cut(s) 710
RsaI GTAC 2 cut(s) 322, 754
RsaNI GTAC 2 cut(s) 321, 753
RseI CAYNNNNRTG 1 cut(s) 534
SalI GTCGAC 1 cut(s) 501
SapI GCTCTTC 1 cut(s) 396
SaqAI TTAA 2 cut(s) 245, 957
SatI GCNGC 8 cut(s) 31, 63, 233, 426, 429, 432, 435, 1155
Sau3AI GATC 5 cut(s) 139, 568, 710, 940, 1049
Sau96I GGNCC 6 cut(s) 43, 472, 668, 686, 803, 947
ScaI AGTACT 2 cut(s) 322, 754
SchI GAGTC 1 cut(s) 884
ScrFI CCNGG 2 cut(s) 15, 515
SduI GDGCHC 2 cut(s) 451, 893
SfaNI GCATC 3 cut(s) 91, 379, 713
Sfr274I CTCGAG 1 cut(s) 651
SinI GGWCC 3 cut(s) 668, 686, 947
SlaI CTCGAG 1 cut(s) 651
SmiMI CAYNNNNRTG 1 cut(s) 534
SmlI CTYRAG 2 cut(s) 119, 651
SmoI CTYRAG 2 cut(s) 119, 651
Sse9I AATT 5 cut(s) 170, 411, 509, 1117, 1171
SsiI CCGC 2 cut(s) 30, 62
SspI AATATT 1 cut(s) 330
SspMI CTAG 3 cut(s) 714, 756, 1164
StyD4I CCNGG 2 cut(s) 13, 513
TaaI ACNGT 5 cut(s) 270, 819, 929, 988, 1067
TaiI ACGT 1 cut(s) 152
TaqI TCGA 5 cut(s) 316, 502, 652, 844, 878
TasI AATT 5 cut(s) 170, 411, 509, 1117, 1171
TatI WGTACW 2 cut(s) 320, 752
TauI GCSGC 2 cut(s) 33, 65
TfiI GAWTC 3 cut(s) 846, 1032, 1079
Tru1I TTAA 2 cut(s) 245, 957
Tru9I TTAA 2 cut(s) 245, 957
TscAI CASTG 3 cut(s) 188, 856, 861
TseFI GTSAC 2 cut(s) 618, 655
TseI GCWGC 6 cut(s) 232, 425, 428, 431, 434, 1154
Tsp45I GTSAC 2 cut(s) 618, 655
TspDTI ATGAA 6 cut(s) 164, 183, 323, 644, 878, 987
TspGWI ACGGA 2 cut(s) 589, 606
TspRI CASTG 3 cut(s) 188, 856, 861
Van91I CCANNNNNTGG 1 cut(s) 347
VpaK11BI GGWCC 3 cut(s) 668, 686, 947
XapI RAATTY 2 cut(s) 170, 1117
XceI RCATGY 2 cut(s) 96, 1152
XcmI CCANNNNNNNNNTGG 1 cut(s) 797
XhoI CTCGAG 1 cut(s) 651
XmiI GTMKAC 1 cut(s) 502
XspI CTAG 3 cut(s) 714, 756, 1164
ZrmI AGTACT 2 cut(s) 322, 754
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.