Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0008735

Physical Location & Seq

Genomic Coordinates Reverse (-)

20896061 .. 20903822

Gene Structure 7762 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1176400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1173 bp

ATGGAGGTTCCGGGAATAGAAGAGACCAACAAATCCGATGCCCATTTGACATCAGCAGCAGCTTTTGTGGAGGGAGGAATCCAGGAAGCCTGTGATGATGCTTGCAGTATATGCCTAGAGGCCTTCTGTGAAAATGATCCTTCTACGTTGACGGGTTGCAAGCATGAATTTCATCTTCAGTGCATTCTTGAGTGGTGTCAGCGAAGCTCCCAATGTCCTATGTGTTGGCAACCCATCAGCTTGAAGGATCCCACTGGCCAAGAACTGCTAGAGGCAGTAGTGCAAGAGAGAAGTATTAGGTCTAATCCACCTCGAAATACCACAATATTTCATCATCCAACTCTTGGTGATTTTGAATTGCAGCATTTACCTATGGGTGTAAATGATGCTGAACTTGAAGAGCGTATAATTCAGCACTTAGCTGCTGCTGCTGCAATGGGTCGTTCACGCCACATTGCCAGAAGGGAAGGACAAAGAAATAGGTCATCAGCTCAAGGTCGCCCGCAATACCTGGTCTTCTCTACCCATCCTAACACACCTCCGGCTCCTGTTTCATCTTCCCCTGATCAGATGGAAGAAGGTGAACCGGCTCCTTCAGTTGCAGTAACTTTCCCTTCGCCTCCTACAAATATAGGGGAAGAATCGTCTCAAATGGCCACTCCTGTACATTCATCACAATCTGGTCCTATGGCTGCCTCAGTGTCTGGATCTAGTGTTTCTTCTCCAAATCAACAAGGATCTTCCATGAGCAATAGGAGGTCACCTAGCCAGTCATCCCCAAGTACCCAAGATAGAGCTGGCCCATCAGACATGCAATCATTTTCAGACTCTCTGAAAGCTCGATTTAGTGCAGTATCAACGAGATACAAAGAGTCAATATCAAAAAGCACAAAAGGTTGGAAGGAGAGATTGTTCTCTCGCAATACTTCCATGGCAGATCTTGGTTCGGAAGTTAAAAGAGAGGTTAATGCAGGGTTTGAAACTGTATCACGCATGATGGAGCGTCTGGAAACAAAAGACAATAACAGAGTTAATGATGCTTCTGTGTCAAATAGTTTAGACAGTCCGGTTTCTGGATCAGACAATCGACAGATATCTGAAAGTGGTGGTGAAATTTTTTTAAATGACCACAATAAACCAGCTGCTTGTGCTACAGGTTCTACTTCAAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009553 GO:0009555 GO:0009561 GO:0009987 GO:0010498 GO:0016020 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0032501 GO:0032502 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044464 GO:0048229 GO:0048856 GO:0050789 GO:0050794 GO:0051603 GO:0051726 GO:0055046 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_11 zf-RING_2

Protein Analysis

391

Amino Acids

42.19

Weight (kDa)

5.36

Isoelectric Point (pI)

70.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	29 - 75	1.2e-06	RING-H2 zinc finger domain
zf-RING_2	PF13639	34 - 75	1.9e-09	Ring finger domain
zf-RING_11	PF17123	35 - 62	4e-07	RING-like zinc finger
zf-C3HC4_2	PF13923	35 - 75	3.3e-06	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008735)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G22000 AT5G22000 AT5G22000 AT5G22000
fragaria_vesca	FvH4_3g26760 FvH4_3g26760
malus_domestica	MD03G1160200.v1.1 MD11G1176400.v1.1
prunus_persica	Prupe.6G148500_v2.0.a1 Prupe.6G148500_v2.0.a1 Prupe.6G148500_v2.0.a1
pyrus_communis	pycom03g11270 pycom11g15060
rosa_chinensis	RchiOBHm_Chr5g0049191
rosa_laevigata	RLG00000034598
rosa_multiflora	Rmu_sc0001440.1_g000001
rosa_roxburghii	Rroxscaffold_1G00032540
rosa_rugosa	Rorug05G0245500 Rorug05G0245600.1
rosa_samantha	Rh5AG323500 Rh5BG333900 Rh5DG345600
rosa_wichuraiana	Rw5G030550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	344
AciI	CCGC	1 cut(s)	503
AclWI	GGATC	6 cut(s)	131, 242, 255, 715, 745, 1084
AcoI	YGGCCR	2 cut(s)	256, 654
AcsI	RAATTY	2 cut(s)	167, 1113
AcuI	CTGAAG	2 cut(s)	161, 579
AfaI	GTAC	2 cut(s)	666, 784
AfiI	CCNNNNNNNGG	2 cut(s)	344, 1073
AgsI	TTSAA	5 cut(s)	244, 356, 398, 980, 1167
AjnI	CCWGG	2 cut(s)	81, 510
AloI	GAACNNNNNNTCC	2 cut(s)	896, 928
AluBI	AGCT	8 cut(s)	62, 207, 240, 422, 491, 797, 839, 1142
AluI	AGCT	8 cut(s)	62, 207, 240, 422, 491, 797, 839, 1142
Alw26I	GTCTC	2 cut(s)	17, 651
AlwI	GGATC	6 cut(s)	131, 242, 255, 715, 745, 1084
AlwNI	CAGNNNCTG	1 cut(s)	704
AoxI	GGCC	4 cut(s)	120, 256, 654, 799
ApeKI	GCWGC	9 cut(s)	56, 59, 361, 422, 425, 428, 431, 692, 1142
ApoI	RAATTY	2 cut(s)	167, 1113
AspS9I	GGNCC	2 cut(s)	683, 800
AsuC2I	CCSGG	1 cut(s)	12
AsuHPI	GGTGA	4 cut(s)	359, 593, 753, 1121
AvaII	GGWCC	1 cut(s)	683
BalI	TGGCCA	2 cut(s)	258, 656
BamHI	GGATCC	1 cut(s)	247
BbsI	GAAGAC	1 cut(s)	508
BbvI	GCAGC	9 cut(s)	68, 71, 373, 409, 412, 415, 418, 679, 1129
BccI	CCATC	5 cut(s)	242, 534, 565, 811, 991
BciT130I	CCWGG	2 cut(s)	83, 512
BclI	TGATCA	1 cut(s)	565
BcnI	CCSGG	1 cut(s)	12
BcoDI	GTCTC	2 cut(s)	17, 651
BfaI	CTAG	4 cut(s)	116, 269, 711, 765
BfmI	CTRYAG	1 cut(s)	1152
BglII	AGATCT	1 cut(s)	937
BisI	GCNGC	9 cut(s)	57, 60, 362, 423, 426, 429, 432, 693, 1143
BlsI	GCNGC	9 cut(s)	58, 61, 363, 424, 427, 430, 433, 694, 1144
Bme1390I	CCNGG	3 cut(s)	12, 83, 512
Bme18I	GGWCC	1 cut(s)	683
BmgT120I	GGNCC	2 cut(s)	683, 800
BmiI	GGNNCC	4 cut(s)	9, 249, 546, 591
BmrFI	CCNGG	3 cut(s)	12, 83, 512
BmsI	GCATC	4 cut(s)	28, 88, 376, 1027
BpiI	GAAGAC	1 cut(s)	508
BpuEI	CTTGAG	2 cut(s)	209, 477
BpuMI	CCSGG	1 cut(s)	12
BsaI	GGTCTC	1 cut(s)	17
BsaJI	CCNNGG	1 cut(s)	930
BsaWI	WCCGGW	1 cut(s)	1066
BsaXI	ACNNNNNCTCC	2 cut(s)	896, 926
Bsc4I	CCNNNNNNNGG	2 cut(s)	344, 1073
Bse118I	RCCGGY	1 cut(s)	586
Bse1I	ACTGG	2 cut(s)	259, 769
Bse3DI	GCAATG	2 cut(s)	441, 453
BseBI	CCWGG	2 cut(s)	83, 512
BseDI	CCNNGG	1 cut(s)	930
BseGI	GGATG	3 cut(s)	334, 526, 773
BseLI	CCNNNNNNNGG	2 cut(s)	344, 1073
BseMI	GCAATG	2 cut(s)	441, 453
BseMII	CTCAG	1 cut(s)	711
BseNI	ACTGG	2 cut(s)	259, 769
BseXI	GCAGC	9 cut(s)	68, 71, 373, 409, 412, 415, 418, 679, 1129
BsgI	GTGCAG	1 cut(s)	870
BshFI	GGCC	4 cut(s)	122, 258, 656, 801
BsiSI	CCGG	4 cut(s)	11, 542, 587, 1067
BslI	CCNNNNNNNGG	2 cut(s)	344, 1073
BsmAI	GTCTC	2 cut(s)	17, 651
BsmBI	CGTCTC	1 cut(s)	651
BsmI	GAATGC	1 cut(s)	183
BsnI	GGCC	4 cut(s)	122, 258, 656, 801
Bso31I	GGTCTC	1 cut(s)	17
Bsp1407I	TGTACA	1 cut(s)	664
Bsp143I	GATC	7 cut(s)	136, 247, 565, 707, 737, 937, 1076
Bsp19I	CCATGG	1 cut(s)	930
BspACI	CCGC	1 cut(s)	503
BspANI	GGCC	4 cut(s)	122, 258, 656, 801
BspCNI	CTCAG	1 cut(s)	710
BspLI	GGNNCC	4 cut(s)	9, 249, 546, 591
BspPI	GGATC	6 cut(s)	131, 242, 255, 715, 745, 1084
BspQI	GCTCTTC	1 cut(s)	393
BspTNI	GGTCTC	1 cut(s)	17
BsrDI	GCAATG	2 cut(s)	441, 453
BsrFI	RCCGGY	1 cut(s)	586
BsrGI	TGTACA	1 cut(s)	664
BsrI	ACTGG	2 cut(s)	259, 769
BssAI	RCCGGY	1 cut(s)	586
BssECI	CCNNGG	1 cut(s)	930
BssMI	GATC	7 cut(s)	136, 247, 565, 707, 737, 937, 1076
BssT1I	CCWWGG	1 cut(s)	930
Bst2UI	CCWGG	2 cut(s)	83, 512
Bst4CI	ACNGT	2 cut(s)	985, 1064
Bst6I	CTCTTC	2 cut(s)	15, 393
BstAPI	GCANNNNNTGC	1 cut(s)	111
BstAUI	TGTACA	1 cut(s)	664
BstC8I	GCNNGC	4 cut(s)	103, 161, 503, 799
BstDEI	CTNAG	2 cut(s)	418, 697
BstDSI	CCRYGG	1 cut(s)	930
BstEII	GGTNACC	1 cut(s)	759
BstF5I	GGATG	3 cut(s)	334, 526, 773
BstKTI	GATC	7 cut(s)	139, 250, 568, 710, 740, 940, 1079
BstMAI	GTCTC	2 cut(s)	17, 651
BstMBI	GATC	7 cut(s)	136, 247, 565, 707, 737, 937, 1076
BstMWI	GCNNNNNNNGC	4 cut(s)	111, 428, 431, 1148
BstNI	CCWGG	2 cut(s)	83, 512
BstNSI	RCATGY	1 cut(s)	814
BstPI	GGTNACC	1 cut(s)	759
BstSCI	CCNGG	3 cut(s)	10, 81, 510
BstSFI	CTRYAG	1 cut(s)	1152
BstV1I	GCAGC	9 cut(s)	68, 71, 373, 409, 412, 415, 418, 679, 1129
BstV2I	GAAGAC	1 cut(s)	508
BstX2I	RGATCY	4 cut(s)	247, 707, 737, 937
BstYI	RGATCY	4 cut(s)	247, 707, 737, 937
BsuRI	GGCC	4 cut(s)	122, 258, 656, 801
BtgI	CCRYGG	1 cut(s)	930
BtsCI	GGATG	3 cut(s)	334, 526, 773
BtsIMutI	CAGTG	3 cut(s)	185, 252, 705
Cac8I	GCNNGC	4 cut(s)	103, 161, 503, 799
CaiI	CAGNNNCTG	1 cut(s)	704
Cfr10I	RCCGGY	1 cut(s)	586
Cfr13I	GGNCC	2 cut(s)	683, 800
CseI	GACGC	1 cut(s)	992
CsiI	ACCWGGT	1 cut(s)	510
Csp6I	GTAC	2 cut(s)	665, 783
CviAII	CATG	5 cut(s)	164, 745, 811, 931, 994
CviQI	GTAC	2 cut(s)	665, 783
DdeI	CTNAG	2 cut(s)	418, 697
DpnI	GATC	7 cut(s)	138, 249, 567, 709, 739, 939, 1078
DpnII	GATC	7 cut(s)	136, 247, 565, 707, 737, 937, 1076
DraI	TTTAAA	1 cut(s)	1122
EaeI	YGGCCR	2 cut(s)	256, 654
Eam1104I	CTCTTC	2 cut(s)	15, 393
EarI	CTCTTC	2 cut(s)	15, 393
Eco130I	CCWWGG	1 cut(s)	930
Eco147I	AGGCCT	1 cut(s)	122
Eco31I	GGTCTC	1 cut(s)	17
Eco32I	GATATC	1 cut(s)	1095
Eco47I	GGWCC	1 cut(s)	683
Eco57I	CTGAAG	2 cut(s)	161, 579
Eco91I	GGTNACC	1 cut(s)	759
EcoO65I	GGTNACC	1 cut(s)	759
EcoRII	CCWGG	2 cut(s)	81, 510
EcoRV	GATATC	1 cut(s)	1095
EcoT14I	CCWWGG	1 cut(s)	930
ErhI	CCWWGG	1 cut(s)	930
Esp3I	CGTCTC	1 cut(s)	651
FaeI	CATG	5 cut(s)	167, 748, 814, 934, 997
FatI	CATG	5 cut(s)	163, 744, 810, 930, 993
FauI	CCCGC	1 cut(s)	510
FbaI	TGATCA	1 cut(s)	565
Fnu4HI	GCNGC	9 cut(s)	57, 60, 362, 423, 426, 429, 432, 693, 1143
FokI	GGATG	3 cut(s)	321, 513, 760
Fsp4HI	GCNGC	9 cut(s)	57, 60, 362, 423, 426, 429, 432, 693, 1143
FspBI	CTAG	4 cut(s)	116, 269, 711, 765
GluI	GCNGC	9 cut(s)	57, 60, 362, 423, 426, 429, 432, 693, 1143
HaeIII	GGCC	4 cut(s)	122, 258, 656, 801
HapII	CCGG	4 cut(s)	11, 542, 587, 1067
HgaI	GACGC	1 cut(s)	992
Hin1II	CATG	5 cut(s)	167, 748, 814, 934, 997
HincII	GTYRAC	1 cut(s)	150
HindII	GTYRAC	1 cut(s)	150
HinfI	GANTC	4 cut(s)	78, 641, 827, 872
HpaII	CCGG	4 cut(s)	11, 542, 587, 1067
HphI	GGTGA	4 cut(s)	359, 593, 753, 1121
Hpy166II	GTNNAC	3 cut(s)	150, 446, 584
Hpy188I	TCNGA	8 cut(s)	37, 570, 808, 826, 834, 949, 1081, 1099
Hpy188III	TCNNGA	4 cut(s)	188, 705, 1007, 1074
Hpy8I	GTNNAC	3 cut(s)	150, 446, 584
HpyAV	CCTTC	9 cut(s)	133, 150, 238, 456, 461, 572, 603, 624, 895
HpyCH4III	ACNGT	2 cut(s)	985, 1064
HpyCH4IV	ACGT	1 cut(s)	146
HpyF10VI	GCNNNNNNNGC	4 cut(s)	111, 428, 431, 1148
HpyF3I	CTNAG	2 cut(s)	418, 697
HpySE526I	ACGT	1 cut(s)	146
Hsp92II	CATG	5 cut(s)	167, 748, 814, 934, 997
Ksp22I	TGATCA	1 cut(s)	565
Kzo9I	GATC	7 cut(s)	136, 247, 565, 707, 737, 937, 1076
LguI	GCTCTTC	1 cut(s)	393
LmnI	GCTCC	4 cut(s)	212, 550, 595, 1000
Lsp1109I	GCAGC	9 cut(s)	68, 71, 373, 409, 412, 415, 418, 679, 1129
LweI	GCATC	4 cut(s)	28, 88, 376, 1027
MabI	ACCWGGT	1 cut(s)	510
MaeI	CTAG	4 cut(s)	116, 269, 711, 765
MaeII	ACGT	1 cut(s)	146
MaeIII	GTNAC	2 cut(s)	604, 759
MalI	GATC	7 cut(s)	138, 249, 567, 709, 739, 939, 1078
MboI	GATC	7 cut(s)	136, 247, 565, 707, 737, 937, 1076
MboII	GAAGA	9 cut(s)	32, 167, 410, 508, 549, 587, 650, 711, 732
MflI	RGATCY	4 cut(s)	247, 707, 737, 937
MlsI	TGGCCA	2 cut(s)	258, 656
MluCI	AATT	5 cut(s)	167, 356, 408, 1113, 1168
MluNI	TGGCCA	2 cut(s)	258, 656
MlyI	GAGTC	2 cut(s)	821, 881
MmeI	TCCRAC	2 cut(s)	362, 878
Mox20I	TGGCCA	2 cut(s)	258, 656
MscI	TGGCCA	2 cut(s)	258, 656
MseI	TTAA	4 cut(s)	954, 966, 1032, 1121
Msp20I	TGGCCA	2 cut(s)	258, 656
MspA1I	CMGCKG	1 cut(s)	1142
MspI	CCGG	4 cut(s)	11, 542, 587, 1067
MspR9I	CCNGG	3 cut(s)	12, 83, 512
Mva1269I	GAATGC	1 cut(s)	183
MvaI	CCWGG	2 cut(s)	83, 512
MwoI	GCNNNNNNNGC	4 cut(s)	111, 428, 431, 1148
NciI	CCSGG	1 cut(s)	12
NcoI	CCATGG	1 cut(s)	930
NdeII	GATC	7 cut(s)	136, 247, 565, 707, 737, 937, 1076
NlaIII	CATG	5 cut(s)	167, 748, 814, 934, 997
NlaIV	GGNNCC	4 cut(s)	9, 249, 546, 591
NmuCI	GTSAC	1 cut(s)	759
NspI	RCATGY	1 cut(s)	814
PceI	AGGCCT	1 cut(s)	122
PciSI	GCTCTTC	1 cut(s)	393
PctI	GAATGC	1 cut(s)	183
PfeI	GAWTC	2 cut(s)	78, 641
PflMI	CCANNNNNTGG	1 cut(s)	344
PfoI	TCCNGGA	2 cut(s)	10, 81
PkrI	GCNGC	9 cut(s)	58, 61, 363, 424, 427, 430, 433, 694, 1144
PleI	GAGTC	2 cut(s)	821, 880
PpsI	GAGTC	2 cut(s)	821, 880
Psp6I	CCWGG	2 cut(s)	81, 510
PspEI	GGTNACC	1 cut(s)	759
PspGI	CCWGG	2 cut(s)	81, 510
PspN4I	GGNNCC	4 cut(s)	9, 249, 546, 591
PspPI	GGNCC	2 cut(s)	683, 800
PstNI	CAGNNNCTG	1 cut(s)	704
PsuI	RGATCY	4 cut(s)	247, 707, 737, 937
PvuII	CAGCTG	1 cut(s)	1142
RsaI	GTAC	2 cut(s)	666, 784
RsaNI	GTAC	2 cut(s)	665, 783
SapI	GCTCTTC	1 cut(s)	393
SaqAI	TTAA	4 cut(s)	954, 966, 1032, 1121
SatI	GCNGC	9 cut(s)	57, 60, 362, 423, 426, 429, 432, 693, 1143
Sau3AI	GATC	7 cut(s)	136, 247, 565, 707, 737, 937, 1076
Sau96I	GGNCC	2 cut(s)	683, 800
SchI	GAGTC	2 cut(s)	821, 881
ScrFI	CCNGG	3 cut(s)	12, 83, 512
SexAI	ACCWGGT	1 cut(s)	510
SfaNI	GCATC	4 cut(s)	28, 88, 376, 1027
SfcI	CTRYAG	1 cut(s)	1152
SinI	GGWCC	1 cut(s)	683
SmlI	CTYRAG	2 cut(s)	188, 492
SmoI	CTYRAG	2 cut(s)	188, 492
Sse9I	AATT	5 cut(s)	167, 356, 408, 1113, 1168
SseBI	AGGCCT	1 cut(s)	122
SsiI	CCGC	1 cut(s)	503
SspI	AATATT	1 cut(s)	327
SspMI	CTAG	4 cut(s)	116, 269, 711, 765
StuI	AGGCCT	1 cut(s)	122
StyD4I	CCNGG	3 cut(s)	10, 81, 510
StyI	CCWWGG	1 cut(s)	930
TaaI	ACNGT	2 cut(s)	985, 1064
TaiI	ACGT	1 cut(s)	149
TaqI	TCGA	3 cut(s)	313, 841, 1087
TasI	AATT	5 cut(s)	167, 356, 408, 1113, 1168
TatI	WGTACW	1 cut(s)	664
TfiI	GAWTC	2 cut(s)	78, 641
Tru1I	TTAA	4 cut(s)	954, 966, 1032, 1121
Tru9I	TTAA	4 cut(s)	954, 966, 1032, 1121
TscAI	CASTG	3 cut(s)	185, 259, 705
TseFI	GTSAC	1 cut(s)	759
TseI	GCWGC	9 cut(s)	56, 59, 361, 422, 425, 428, 431, 692, 1142
Tsp45I	GTSAC	1 cut(s)	759
TspDTI	ATGAA	5 cut(s)	161, 180, 320, 543, 660
TspRI	CASTG	3 cut(s)	185, 259, 705
Van91I	CCANNNNNTGG	1 cut(s)	344
VpaK11BI	GGWCC	1 cut(s)	683
XapI	RAATTY	2 cut(s)	167, 1113
XceI	RCATGY	1 cut(s)	814
XcmI	CCANNNNNNNNNTGG	1 cut(s)	794
XspI	CTAG	4 cut(s)	116, 269, 711, 765

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.