MD03G1160300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0017632
Physical Location & Seq
Reverse (-)
19132905 .. 19133321
417 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1160300.v1.1.491

Sequence Viewer

Length: 417 bp
ATGGCCCGGGACCCCCTCTTTCAATCGCCAAAGTTGACTCGGCCGCCGCCCCGAGAGAAGCGGCGAGTCGGAGAAGTGGCCGGAGGCGCGGCAGCCGAGTGTGCGGCGGTGTGTTGCTGCTGCCCGTGCAGTGTAATGAATTTGCTTATATTGGCGGTGTACAAGGCCCCGAAGGGGCTGTGCATGAAGGCCTGGGCCCACACGAAAAAGAAACGACGGTGCGCGGTTCAGAAGAAGGGACGGCTCGGCGGCGGTGGCGACGACGACGACGGGAAGAGAGCGAGCAGCTGTACAGAAGGTGACGGAGAGAAGAAGAGCAACGCCGAAACCGAGGGCGCTGATTTGTTGGAGAAGGAGATGTGGGACCGATTTCACGGTGCCGGATTTTGGAGGAGTGCTTCCCGGAGAGAATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

139

Amino Acids

15.0

Weight (kDa)

9.25

Isoelectric Point (pI)

57.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017632)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g26780
malus_domestica MD03G1160300.v1.1 MD15G1108700.v1.1
prunus_persica Prupe.6G148600_v2.0.a1
pyrus_communis pycom03g11280 pycom08g10970 pycom15g09930
rosa_chinensis RchiOBHm_Chr5g0049201
rosa_rugosa Rorug05G0245700
rosa_samantha Rh5BG334000 Rh5DG346100
rosa_wichuraiana Rw5G030560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 377
AccII CGCG 2 cut(s) 89, 224
AcoI YGGCCR 2 cut(s) 41, 78
AcsI RAATTY 1 cut(s) 139
AfaI GTAC 2 cut(s) 161, 292
AfiI CCNNNNNNNGG 2 cut(s) 174, 387
AgsI TTSAA 1 cut(s) 23
AjnI CCWGG 1 cut(s) 191
AluBI AGCT 1 cut(s) 288
AluI AGCT 1 cut(s) 288
Ama87I CYCGRG 2 cut(s) 6, 51
AoxI GGCC 6 cut(s) 3, 41, 78, 165, 189, 195
ApaI GGGCCC 1 cut(s) 199
ApeKI GCWGC 4 cut(s) 92, 117, 120, 285
ApoI RAATTY 1 cut(s) 139
AspLEI GCGC 3 cut(s) 89, 224, 338
AspS9I GGNCC 6 cut(s) 4, 10, 166, 195, 196, 364
AsuC2I CCSGG 3 cut(s) 7, 8, 403
AsuHPI GGTGA 1 cut(s) 311
AvaI CYCGRG 2 cut(s) 6, 51
AvaII GGWCC 2 cut(s) 10, 364
BaeGI GKGCMC 1 cut(s) 199
BanI GGYRCC 1 cut(s) 377
BanII GRGCYC 1 cut(s) 199
BbvI GCAGC 4 cut(s) 104, 104, 107, 297
BceAI ACGGC 1 cut(s) 257
BciT130I CCWGG 1 cut(s) 193
BcnI CCSGG 3 cut(s) 7, 8, 403
BfoI RGCGCY 1 cut(s) 339
Bme1390I CCNGG 4 cut(s) 7, 8, 193, 403
Bme18I GGWCC 2 cut(s) 10, 364
BmeT110I CYCGRG 2 cut(s) 6, 51
BmgT120I GGNCC 6 cut(s) 4, 10, 166, 195, 196, 364
BmiI GGNNCC 6 cut(s) 11, 12, 168, 197, 365, 379
BmrFI CCNGG 4 cut(s) 7, 8, 193, 403
BpuMI CCSGG 3 cut(s) 7, 8, 403
BsaJI CCNNGG 3 cut(s) 6, 192, 330
Bsc4I CCNNNNNNNGG 2 cut(s) 174, 387
BseBI CCWGG 1 cut(s) 193
BseDI CCNNGG 3 cut(s) 6, 192, 330
BseLI CCNNNNNNNGG 2 cut(s) 174, 387
BseRI GAGGAG 1 cut(s) 406
BseSI GKGCMC 1 cut(s) 199
BseX3I CGGCCG 1 cut(s) 41
BseXI GCAGC 4 cut(s) 104, 104, 107, 297
BsgI GTGCAG 1 cut(s) 148
Bsh1236I CGCG 2 cut(s) 89, 224
Bsh1285I CGRYCG 1 cut(s) 44
BshFI GGCC 6 cut(s) 5, 43, 80, 167, 191, 197
BshNI GGYRCC 1 cut(s) 377
BsiEI CGRYCG 1 cut(s) 44
BsiHKCI CYCGRG 2 cut(s) 6, 51
BsiSI CCGG 4 cut(s) 7, 81, 381, 403
BslFI GGGAC 3 cut(s) 23, 252, 377
BslI CCNNNNNNNGG 2 cut(s) 174, 387
BsmFI GGGAC 3 cut(s) 23, 252, 377
BsnI GGCC 6 cut(s) 5, 43, 80, 167, 191, 197
BsoBI CYCGRG 2 cut(s) 6, 51
Bsp120I GGGCCC 1 cut(s) 195
Bsp1286I GDGCHC 1 cut(s) 199
Bsp1407I TGTACA 2 cut(s) 159, 290
BspANI GGCC 6 cut(s) 5, 43, 80, 167, 191, 197
BspFNI CGCG 2 cut(s) 89, 224
BspLI GGNNCC 6 cut(s) 11, 12, 168, 197, 365, 379
BspQI GCTCTTC 1 cut(s) 308
BspT107I GGYRCC 1 cut(s) 377
BsrGI TGTACA 2 cut(s) 159, 290
BssECI CCNNGG 3 cut(s) 6, 192, 330
Bst2UI CCWGG 1 cut(s) 193
Bst4CI ACNGT 2 cut(s) 219, 377
Bst6I CTCTTC 2 cut(s) 269, 308
BstAUI TGTACA 2 cut(s) 159, 290
BstC8I GCNNGC 1 cut(s) 283
BstFNI CGCG 2 cut(s) 89, 224
BstH2I RGCGCY 1 cut(s) 339
BstHHI GCGC 3 cut(s) 89, 224, 338
BstMCI CGRYCG 1 cut(s) 44
BstMWI GCNNNNNNNGC 4 cut(s) 86, 101, 126, 255
BstNI CCWGG 1 cut(s) 193
BstSCI CCNGG 4 cut(s) 5, 6, 191, 401
BstSLI GKGCMC 1 cut(s) 199
BstUI CGCG 2 cut(s) 89, 224
BstV1I GCAGC 4 cut(s) 104, 104, 107, 297
BstZI CGGCCG 1 cut(s) 41
BsuRI GGCC 6 cut(s) 5, 43, 80, 167, 191, 197
BtsI GCAGTG 1 cut(s) 136
BtsIMutI CAGTG 1 cut(s) 136
Cac8I GCNNGC 1 cut(s) 283
CfoI GCGC 3 cut(s) 89, 224, 338
Cfr13I GGNCC 6 cut(s) 4, 10, 166, 195, 196, 364
Cfr9I CCCGGG 1 cut(s) 6
Csp6I GTAC 2 cut(s) 160, 291
CviAII CATG 1 cut(s) 184
CviQI GTAC 2 cut(s) 160, 291
EaeI YGGCCR 2 cut(s) 41, 78
EagI CGGCCG 1 cut(s) 41
Eam1104I CTCTTC 2 cut(s) 269, 308
EarI CTCTTC 2 cut(s) 269, 308
EclXI CGGCCG 1 cut(s) 41
Eco147I AGGCCT 1 cut(s) 191
Eco24I GRGCYC 1 cut(s) 199
Eco47I GGWCC 2 cut(s) 10, 364
Eco52I CGGCCG 1 cut(s) 41
Eco88I CYCGRG 2 cut(s) 6, 51
EcoO109I RGGNCCY 2 cut(s) 10, 166
EcoRII CCWGG 1 cut(s) 191
EcoT38I GRGCYC 1 cut(s) 199
FaeI CATG 1 cut(s) 187
FaiI YATR 2 cut(s) 149, 185
FaqI GGGAC 3 cut(s) 23, 252, 377
FatI CATG 1 cut(s) 183
FriOI GRGCYC 1 cut(s) 199
GlaI GCGC 3 cut(s) 88, 223, 337
HaeII RGCGCY 1 cut(s) 339
HaeIII GGCC 6 cut(s) 5, 43, 80, 167, 191, 197
HapII CCGG 4 cut(s) 7, 81, 381, 403
HhaI GCGC 3 cut(s) 89, 224, 338
Hin1II CATG 1 cut(s) 187
Hin6I GCGC 3 cut(s) 87, 222, 336
HinP1I GCGC 3 cut(s) 87, 222, 336
HincII GTYRAC 1 cut(s) 36
HindII GTYRAC 1 cut(s) 36
HinfI GANTC 3 cut(s) 37, 66, 410
HpaII CCGG 4 cut(s) 7, 81, 381, 403
HphI GGTGA 1 cut(s) 311
Hpy166II GTNNAC 2 cut(s) 36, 160
Hpy188I TCNGA 2 cut(s) 71, 231
Hpy188III TCNNGA 1 cut(s) 414
Hpy8I GTNNAC 2 cut(s) 36, 160
Hpy99I CGWCG 5 cut(s) 219, 263, 266, 269, 272
HpyAV CCTTC 5 cut(s) 166, 181, 229, 290, 346
HpyCH4III ACNGT 2 cut(s) 219, 377
HpyCH4V TGCA 2 cut(s) 129, 183
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 101, 126, 255
Hsp92II CATG 1 cut(s) 187
HspAI GCGC 3 cut(s) 87, 222, 336
KflI GGGWCCC 1 cut(s) 10
LguI GCTCTTC 1 cut(s) 308
LpnPI CCDG 5 cut(s) 20, 94, 178, 205, 394
Lsp1109I GCAGC 4 cut(s) 104, 104, 107, 297
MaeIII GTNAC 1 cut(s) 299
MboII GAAGA 4 cut(s) 244, 286, 322, 325
MhlI GDGCHC 1 cut(s) 199
MluCI AATT 1 cut(s) 139
MlyI GAGTC 2 cut(s) 31, 75
MmeI TCCRAC 2 cut(s) 49, 327
MnlI CCTC 4 cut(s) 26, 77, 325, 384
MspA1I CMGCKG 1 cut(s) 288
MspI CCGG 4 cut(s) 7, 81, 381, 403
MspR9I CCNGG 4 cut(s) 7, 8, 193, 403
MvaI CCWGG 1 cut(s) 193
MvnI CGCG 2 cut(s) 89, 224
MwoI GCNNNNNNNGC 4 cut(s) 86, 101, 126, 255
NciI CCSGG 3 cut(s) 7, 8, 403
NlaIII CATG 1 cut(s) 187
NlaIV GGNNCC 6 cut(s) 11, 12, 168, 197, 365, 379
NmeAIII GCCGAG 3 cut(s) 19, 121, 225
NmuCI GTSAC 1 cut(s) 299
PceI AGGCCT 1 cut(s) 191
PciSI GCTCTTC 1 cut(s) 308
PcsI WCGNNNNNNNCGW 1 cut(s) 327
PfeI GAWTC 1 cut(s) 410
PfoI TCCNGGA 1 cut(s) 401
PleI GAGTC 2 cut(s) 31, 74
PpsI GAGTC 2 cut(s) 31, 74
PpuMI RGGWCCY 1 cut(s) 10
Psp5II RGGWCCY 1 cut(s) 10
Psp6I CCWGG 1 cut(s) 191
PspGI CCWGG 1 cut(s) 191
PspN4I GGNNCC 6 cut(s) 11, 12, 168, 197, 365, 379
PspOMI GGGCCC 1 cut(s) 195
PspPI GGNCC 6 cut(s) 4, 10, 166, 195, 196, 364
PspPPI RGGWCCY 1 cut(s) 10
PvuII CAGCTG 1 cut(s) 288
RsaI GTAC 2 cut(s) 161, 292
RsaNI GTAC 2 cut(s) 160, 291
SapI GCTCTTC 1 cut(s) 308
Sau96I GGNCC 6 cut(s) 4, 10, 166, 195, 196, 364
SchI GAGTC 2 cut(s) 31, 75
ScrFI CCNGG 4 cut(s) 7, 8, 193, 403
SduI GDGCHC 1 cut(s) 199
SetI ASST 2 cut(s) 290, 301
SinI GGWCC 2 cut(s) 10, 364
SmaI CCCGGG 1 cut(s) 8
Sse9I AATT 1 cut(s) 139
SseBI AGGCCT 1 cut(s) 191
StuI AGGCCT 1 cut(s) 191
StyD4I CCNGG 4 cut(s) 5, 6, 191, 401
TaaI ACNGT 2 cut(s) 219, 377
TaqII GACCGA 1 cut(s) 381
TasI AATT 1 cut(s) 139
TatI WGTACW 2 cut(s) 159, 290
TauI GCSGC 6 cut(s) 46, 49, 64, 92, 107, 252
TfiI GAWTC 1 cut(s) 410
TscAI CASTG 1 cut(s) 136
TseFI GTSAC 1 cut(s) 299
TseI GCWGC 4 cut(s) 92, 117, 120, 285
Tsp45I GTSAC 1 cut(s) 299
TspDTI ATGAA 2 cut(s) 152, 200
TspGWI ACGGA 1 cut(s) 318
TspMI CCCGGG 1 cut(s) 6
TspRI CASTG 1 cut(s) 136
VpaK11BI GGWCC 2 cut(s) 10, 364
XapI RAATTY 1 cut(s) 139
XmaI CCCGGG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.