MD15G1108700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0017632
Physical Location & Seq
Forward (+)
7587739 .. 7588203
465 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1108700.v1.1.491

Sequence Viewer

Length: 465 bp
ATGACGCGGGATGCTTTGCTTCAATCGCCACCGCTAACCCGGCCTCCGCCTCGCGAGAGACGCAGGGTCGGCGAGGTGGCAGGCGGCGCCGCGGCTGAGTGTGCGGCGGTGTGCTGCTGCTGCCCGTGCAGCGTGATGAATTTGCTGATACTGACTGTGTACAAGGTCCCGAAAGGGATTTGCATGAAGGCCTTGGCCCACAGGAAGAATAAGCGGCAGTGCTTGGCGGCCCACAGGAAGGCACTGTTGCAGCCCAGGCCCAATGGGCTCGCCGGCGGCGGGCTTTATAGCGACGACGACGACTTCTTCAGGAAAGCGAGCAGTTACAGCGACGGCGACGGCGACGTCGACGACGGGAAGGGGAGCGAGGATGAATCTGAAGCCGCTGAGTTGTTGGAGAAGGAGATATGGGACCGGTTTCACGGTGCCGGATTTTGGAGGAGTGCTTCTCGGATAGATTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

155

Amino Acids

16.77

Weight (kDa)

7.54

Isoelectric Point (pI)

52.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017632)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g26780
malus_domestica MD03G1160300.v1.1 MD15G1108700.v1.1
prunus_persica Prupe.6G148600_v2.0.a1
pyrus_communis pycom03g11280 pycom08g10970 pycom15g09930
rosa_chinensis RchiOBHm_Chr5g0049201
rosa_rugosa Rorug05G0245700
rosa_samantha Rh5BG334000 Rh5DG346100
rosa_wichuraiana Rw5G030560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 344
AatII GACGTC 1 cut(s) 348
AccB1I GGYRCC 2 cut(s) 86, 425
AccI GTMKAC 1 cut(s) 348
AccII CGCG 3 cut(s) 7, 54, 92
AcsI RAATTY 1 cut(s) 139
AcuI CTGAAG 2 cut(s) 292, 399
AcyI GRCGYC 2 cut(s) 87, 345
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 3 cut(s) 238, 279, 435
AgeI ACCGGT 1 cut(s) 414
AgsI TTSAA 1 cut(s) 23
AhdI GACNNNNNGTC 1 cut(s) 65
AjnI CCWGG 1 cut(s) 254
Alw26I GTCTC 1 cut(s) 52
AoxI GGCC 5 cut(s) 41, 189, 195, 228, 257
ApeKI GCWGC 5 cut(s) 114, 117, 120, 129, 250
ApoI RAATTY 1 cut(s) 139
AsiGI ACCGGT 1 cut(s) 414
AspLEI GCGC 1 cut(s) 89
AspS9I GGNCC 5 cut(s) 166, 196, 229, 258, 412
AsuC2I CCSGG 1 cut(s) 40
AvaII GGWCC 2 cut(s) 166, 412
BanI GGYRCC 2 cut(s) 86, 425
BanII GRGCYC 1 cut(s) 270
BbvI GCAGC 5 cut(s) 101, 104, 107, 141, 262
BceAI ACGGC 2 cut(s) 349, 355
BciT130I CCWGG 1 cut(s) 256
BcnI CCSGG 1 cut(s) 40
BcoDI GTCTC 1 cut(s) 52
BfoI RGCGCY 1 cut(s) 90
BglI GCCNNNNNGGC 1 cut(s) 265
Bme1390I CCNGG 2 cut(s) 40, 256
Bme18I GGWCC 2 cut(s) 166, 412
BmeRI GACNNNNNGTC 1 cut(s) 65
BmgT120I GGNCC 5 cut(s) 166, 196, 229, 258, 412
BmiI GGNNCC 4 cut(s) 88, 168, 413, 427
BmrFI CCNGG 2 cut(s) 40, 256
BplI GAGNNNNNCTC 2 cut(s) 433, 465
BpuMI CCSGG 1 cut(s) 40
BsaHI GRCGYC 2 cut(s) 87, 345
BsaJI CCNNGG 3 cut(s) 90, 192, 254
BsaWI WCCGGW 1 cut(s) 414
BsaXI ACNNNNNCTCC 2 cut(s) 28, 58
Bsc4I CCNNNNNNNGG 3 cut(s) 238, 279, 435
Bse118I RCCGGY 2 cut(s) 272, 414
BseBI CCWGG 1 cut(s) 256
BseDI CCNNGG 3 cut(s) 90, 192, 254
BseGI GGATG 2 cut(s) 16, 376
BseLI CCNNNNNNNGG 3 cut(s) 238, 279, 435
BseMII CTCAG 2 cut(s) 87, 378
BseRI GAGGAG 1 cut(s) 454
BseXI GCAGC 5 cut(s) 101, 104, 107, 141, 262
BsgI GTGCAG 1 cut(s) 148
Bsh1236I CGCG 3 cut(s) 7, 54, 92
BshFI GGCC 5 cut(s) 43, 191, 197, 230, 259
BshNI GGYRCC 2 cut(s) 86, 425
BshTI ACCGGT 1 cut(s) 414
BsiSI CCGG 4 cut(s) 40, 273, 415, 429
BslFI GGGAC 2 cut(s) 152, 425
BslI CCNNNNNNNGG 3 cut(s) 238, 279, 435
BsmAI GTCTC 1 cut(s) 52
BsmBI CGTCTC 1 cut(s) 52
BsmFI GGGAC 2 cut(s) 152, 425
BsnI GGCC 5 cut(s) 43, 191, 197, 230, 259
Bsp1286I GDGCHC 1 cut(s) 270
Bsp1407I TGTACA 1 cut(s) 159
Bsp68I TCGCGA 1 cut(s) 54
BspANI GGCC 5 cut(s) 43, 191, 197, 230, 259
BspCNI CTCAG 2 cut(s) 88, 379
BspFNI CGCG 3 cut(s) 7, 54, 92
BspLI GGNNCC 4 cut(s) 88, 168, 413, 427
BspT107I GGYRCC 2 cut(s) 86, 425
BsrFI RCCGGY 2 cut(s) 272, 414
BsrGI TGTACA 1 cut(s) 159
BssAI RCCGGY 2 cut(s) 272, 414
BssECI CCNNGG 3 cut(s) 90, 192, 254
BssNI GRCGYC 2 cut(s) 87, 345
BssT1I CCWWGG 1 cut(s) 192
Bst2UI CCWGG 1 cut(s) 256
Bst4CI ACNGT 3 cut(s) 157, 246, 425
BstACI GRCGYC 2 cut(s) 87, 345
BstAUI TGTACA 1 cut(s) 159
BstC8I GCNNGC 5 cut(s) 82, 270, 274, 281, 319
BstDEI CTNAG 2 cut(s) 96, 387
BstDSI CCRYGG 1 cut(s) 90
BstF5I GGATG 2 cut(s) 16, 376
BstFNI CGCG 3 cut(s) 7, 54, 92
BstH2I RGCGCY 1 cut(s) 90
BstHHI GCGC 1 cut(s) 89
BstMAI GTCTC 1 cut(s) 52
BstNI CCWGG 1 cut(s) 256
BstSCI CCNGG 2 cut(s) 38, 254
BstUI CGCG 3 cut(s) 7, 54, 92
BstV1I GCAGC 5 cut(s) 101, 104, 107, 141, 262
BsuRI GGCC 5 cut(s) 43, 191, 197, 230, 259
BtgI CCRYGG 1 cut(s) 90
BtsCI GGATG 2 cut(s) 16, 376
BtsI GCAGTG 1 cut(s) 224
BtsIMutI CAGTG 2 cut(s) 224, 242
BtuMI TCGCGA 1 cut(s) 54
Cac8I GCNNGC 5 cut(s) 82, 270, 274, 281, 319
CfoI GCGC 1 cut(s) 89
Cfr10I RCCGGY 2 cut(s) 272, 414
Cfr13I GGNCC 5 cut(s) 166, 196, 229, 258, 412
Cfr42I CCGCGG 1 cut(s) 93
CseI GACGC 2 cut(s) 13, 69
Csp6I GTAC 1 cut(s) 160
CspAI ACCGGT 1 cut(s) 414
CviAII CATG 1 cut(s) 184
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 2 cut(s) 96, 387
DinI GGCGCC 1 cut(s) 88
DrdI GACNNNNNNGTC 1 cut(s) 344
DriI GACNNNNNGTC 1 cut(s) 65
DseDI GACNNNNNNGTC 1 cut(s) 344
Eam1105I GACNNNNNGTC 1 cut(s) 65
EciI GGCGGA 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 192
Eco147I AGGCCT 1 cut(s) 191
Eco24I GRGCYC 1 cut(s) 270
Eco47I GGWCC 2 cut(s) 166, 412
Eco57I CTGAAG 2 cut(s) 292, 399
EcoO109I RGGNCCY 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 254
EcoT14I CCWWGG 1 cut(s) 192
EcoT38I GRGCYC 1 cut(s) 270
EgeI GGCGCC 1 cut(s) 88
EheI GGCGCC 1 cut(s) 88
ErhI CCWWGG 1 cut(s) 192
Esp3I CGTCTC 1 cut(s) 52
FaeI CATG 1 cut(s) 187
FaiI YATR 3 cut(s) 185, 288, 409
FaqI GGGAC 2 cut(s) 152, 425
FatI CATG 1 cut(s) 183
FauI CCCGC 1 cut(s) 272
FblI GTMKAC 1 cut(s) 348
FokI GGATG 2 cut(s) 23, 383
FriOI GRGCYC 1 cut(s) 270
GlaI GCGC 1 cut(s) 88
HaeII RGCGCY 1 cut(s) 90
HaeIII GGCC 5 cut(s) 43, 191, 197, 230, 259
HapII CCGG 4 cut(s) 40, 273, 415, 429
HgaI GACGC 2 cut(s) 13, 69
HhaI GCGC 1 cut(s) 89
Hin1I GRCGYC 2 cut(s) 87, 345
Hin1II CATG 1 cut(s) 187
Hin6I GCGC 1 cut(s) 87
HinP1I GCGC 1 cut(s) 87
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HinfI GANTC 2 cut(s) 374, 458
HpaII CCGG 4 cut(s) 40, 273, 415, 429
Hpy166II GTNNAC 2 cut(s) 160, 349
Hpy188I TCNGA 2 cut(s) 379, 453
Hpy188III TCNNGA 4 cut(s) 53, 169, 310, 462
Hpy8I GTNNAC 2 cut(s) 160, 349
Hpy99I CGWCG 9 cut(s) 296, 299, 302, 335, 341, 347, 350, 353, 356
HpyAV CCTTC 4 cut(s) 181, 232, 352, 394
HpyCH4III ACNGT 3 cut(s) 157, 246, 425
HpyCH4IV ACGT 1 cut(s) 345
HpyCH4V TGCA 3 cut(s) 129, 183, 250
HpyF3I CTNAG 2 cut(s) 96, 387
HpySE526I ACGT 1 cut(s) 345
Hsp92I GRCGYC 2 cut(s) 87, 345
Hsp92II CATG 1 cut(s) 187
HspAI GCGC 1 cut(s) 87
KasI GGCGCC 1 cut(s) 86
KroI GCCGGC 1 cut(s) 272
KroNI GCCGGC 1 cut(s) 274
KspI CCGCGG 1 cut(s) 93
LmnI GCTCC 1 cut(s) 363
Lsp1109I GCAGC 5 cut(s) 101, 104, 107, 141, 262
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 1 cut(s) 323
MboII GAAGA 2 cut(s) 217, 298
MhlI GDGCHC 1 cut(s) 270
MluCI AATT 1 cut(s) 139
Mly113I GGCGCC 1 cut(s) 87
MmeI TCCRAC 1 cut(s) 375
MnlI CCTC 5 cut(s) 54, 60, 67, 361, 432
MreI CGCCGGCG 1 cut(s) 272
MroNI GCCGGC 1 cut(s) 272
MspA1I CMGCKG 2 cut(s) 92, 386
MspI CCGG 4 cut(s) 40, 273, 415, 429
MspR9I CCNGG 2 cut(s) 40, 256
MvaI CCWGG 1 cut(s) 256
MvnI CGCG 3 cut(s) 7, 54, 92
NaeI GCCGGC 1 cut(s) 274
NarI GGCGCC 1 cut(s) 87
NciI CCSGG 1 cut(s) 40
NgoMIV GCCGGC 1 cut(s) 272
NlaIII CATG 1 cut(s) 187
NlaIV GGNNCC 4 cut(s) 88, 168, 413, 427
NruI TCGCGA 1 cut(s) 54
PceI AGGCCT 1 cut(s) 191
PcsI WCGNNNNNNNCGW 2 cut(s) 339, 345
PdiI GCCGGC 1 cut(s) 274
PfeI GAWTC 2 cut(s) 374, 458
PinAI ACCGGT 1 cut(s) 414
PluTI GGCGCC 1 cut(s) 90
PpuMI RGGWCCY 1 cut(s) 166
Psp5II RGGWCCY 1 cut(s) 166
Psp6I CCWGG 1 cut(s) 254
PspGI CCWGG 1 cut(s) 254
PspN4I GGNNCC 4 cut(s) 88, 168, 413, 427
PspPI GGNCC 5 cut(s) 166, 196, 229, 258, 412
PspPPI RGGWCCY 1 cut(s) 166
RruI TCGCGA 1 cut(s) 54
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
SacII CCGCGG 1 cut(s) 93
SalI GTCGAC 1 cut(s) 347
Sau96I GGNCC 5 cut(s) 166, 196, 229, 258, 412
ScrFI CCNGG 2 cut(s) 40, 256
SduI GDGCHC 1 cut(s) 270
SetI ASST 3 cut(s) 78, 168, 348
SfoI GGCGCC 1 cut(s) 88
Sfr303I CCGCGG 1 cut(s) 93
SgrAI CRCCGGYG 1 cut(s) 272
SgrBI CCGCGG 1 cut(s) 93
SgrDI CGTCGACG 1 cut(s) 347
SinI GGWCC 2 cut(s) 166, 412
Sse9I AATT 1 cut(s) 139
SseBI AGGCCT 1 cut(s) 191
SspDI GGCGCC 1 cut(s) 86
StuI AGGCCT 1 cut(s) 191
StyD4I CCNGG 2 cut(s) 38, 254
StyI CCWWGG 1 cut(s) 192
TaaI ACNGT 3 cut(s) 157, 246, 425
TaiI ACGT 1 cut(s) 348
TaqI TCGA 1 cut(s) 348
TasI AATT 1 cut(s) 139
TatI WGTACW 1 cut(s) 159
TauI GCSGC 8 cut(s) 87, 92, 95, 107, 217, 230, 279, 386
TfiI GAWTC 2 cut(s) 374, 458
TscAI CASTG 2 cut(s) 224, 249
TseI GCWGC 5 cut(s) 114, 117, 120, 129, 250
TspDTI ATGAA 3 cut(s) 152, 200, 387
TspRI CASTG 2 cut(s) 224, 249
VpaK11BI GGWCC 2 cut(s) 166, 412
XapI RAATTY 1 cut(s) 139
XmiI GTMKAC 1 cut(s) 348
ZraI GACGTC 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.