Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0004420

Physical Location & Seq

Genomic Coordinates Reverse (-)

20387379 .. 20392899

Gene Structure 5521 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1163600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1317 bp

ATGAATCAACCGAAAATCAAGCGTCGGGTTGGTAAATACGAGATTGGAAGGACAATCGGTGAGGGTACATTTGCAAAAGTCAAGTTTGCTAGAAATTCTGAGACTGGGGAGCCTGTGGCTCTCAAAATTCTTGACAAAGAGAAGGTTCTGAAGCACAAGATGGCAGAACAGATCAAGCGGGAAATAGAAACAATGAAGCTGATTAAGCATCCAAATGTTGTTCAGTTGTATGAGGTAATGGGGAGTAAGACAAAGATATTTATAGTTATGGAGTTCGTAACTGGAGGCGAGCTCTTTGACAAAATTGTAAACAATGGACGAATGAAAGAAGATGAAGCACGGAGATATTTTCAACAGCTTATTAATGCAGTTGATTACTGCCATAGCAGAGGCGTCTATCATAGAGATCTCAAGCCAGAAAATTTGCTATTGGATGCATATGGGAACCTTAAAGTTTCTGATTTCGGATTGAGCGCACTATCCCAACAAGTCAGGGATGATGGGTTACTTCACACCACCTGTGGAACTCCAAATTATGTTGCTCCTGAGGTCCTTAACGATAGAGGCTATGATGGGGCAACTGCGGACTTGTGGTCATGTGGAGTGATACTCTTTGTATTGCTTGCAGGTTACTTGCCTTTTGATGATTCGAATCTTATGAACCTGTATAAAAAAATCTCGGCAGCTGAGTTTACTTGTCCCCCGTGGCTCTCTTTTGGTGCCATGAAATTAATAGCTCGAATTTTGGATCCTAACCCTATGACTCGTGTCACTATTGCTGAAATTTTGGAAGATGAATGGTTCAAGAAGGATTACAAGGCACCCGTGTTTGAGGAGAAAGAAAATACAAACTTGGATGATGTAGAAGCTGTTTTTAAGGATTCAGAACACCATGTGACGGAAAAAAAGGAAGAACAACCAGTAGCTATGAATGCTTTTGAGTTAATTTCAATGTCGAAGGGGCTCAACCTTGGGAATTTGTTTGATGTAGAACAGGGATTTAAGAGAGAAACAAGGTTCACATCCAAATGCCCTGCCAATGAGATAATTCATAAAATTGAAGAAGCTGCTAAGCCTCTTGGTTTTGACGTGCATAAAAAAAACTACAAGCTGAGGCTTGAAAATATGAAAGCTGGAAGAAAGGGAAACCTTAATGTTGCCACAGAGATATTTCAAGTTGCACCTTCTCTTCACATGGTCGAAGTGCGAAAAGCTAAAGGGGACACCTTGGAGTTCCACAAGTTCTATAAGAACCTCGCAACAGGCCTGGAGGATGTTGTTTGGAAGACGGAGGAGGATATGCAACAAGTCAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04068 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418

Pfam Domains

Protein Families

NAF Pkinase

Protein Analysis

439

Amino Acids

50.02

Weight (kDa)

6.85

Isoelectric Point (pI)

35.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	13 - 268	2.3e-74	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	14 - 264	2.1e-37	Protein tyrosine and serine/threonine kinase
Kinase-like	PF14531	107 - 216	6.9e-06	Kinase-like
NAM-associated	PF14303	272 - 418	1e-06	No apical meristem-associated C-terminal domain
NAF	PF03822	307 - 366	1.9e-23	NAF domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0004420)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26980 AT2G26980 AT2G26980 AT2G26980 AT5G21326
fragaria_vesca	FvH4_3g27610 FvH4_3g27610 FvH4_3g27610 FvH4_3g27610 FvH4_3g27610 FvH4_3g27610 FvH4_3g27610 FvH4_3g27610
malus_domestica	MD03G1163600.v1.1
prunus_persica	Prupe.6G156900_v2.0.a1 Prupe.6G156900_v2.0.a1 Prupe.6G156900_v2.0.a1 Prupe.6G156900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0050061
rosa_laevigata	RLG00000034683
rosa_multiflora	Rmu_sc0000333.1_g000007
rosa_roxburghii	Rroxscaffold_1G00031070
rosa_rugosa	Rorug05G0257000 Rorug05G0257100 Rorug05G0257200.1 Rorug05G0257300 Rorug05G0257400
rosa_samantha	Rh5AG330200 Rh5BG340900 Rh5CG367500 Rh5DG353700
rosa_wichuraiana	Rw5G031160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	617
AccB1I	GGYRCC	2 cut(s)	719, 820
AciI	CCGC	2 cut(s)	178, 584
AclWI	GGATC	2 cut(s)	743, 756
AcsI	RAATTY	6 cut(s)	94, 126, 421, 741, 783, 976
AcuI	CTGAAG	1 cut(s)	170
AcyI	GRCGYC	1 cut(s)	393
AdeI	CACNNNGTG	1 cut(s)	895
AfaI	GTAC	1 cut(s)	67
AfiI	CCNNNNNNNGG	1 cut(s)	898
AgsI	TTSAA	6 cut(s)	353, 805, 951, 1061, 1121, 1175
AhdI	GACNNNNNGTC	1 cut(s)	592
AjiI	CACGTC	1 cut(s)	1090
AjnI	CCWGG	1 cut(s)	1266
Alw21I	GWGCWC	1 cut(s)	294
Alw26I	GTCTC	1 cut(s)	95
AlwI	GGATC	2 cut(s)	743, 756
AoxI	GGCC	1 cut(s)	1264
ApeKI	GCWGC	2 cut(s)	683, 1067
ApoI	RAATTY	6 cut(s)	94, 126, 421, 741, 783, 976
AseI	ATTAAT	2 cut(s)	363, 731
AspLEI	GCGC	1 cut(s)	476
AspS9I	GGNCC	1 cut(s)	550
AsuHPI	GGTGA	1 cut(s)	71
AsuII	TTCGAA	1 cut(s)	650
AvaII	GGWCC	1 cut(s)	550
AxyI	CCTNAGG	1 cut(s)	546
BamHI	GGATCC	1 cut(s)	748
BanI	GGYRCC	2 cut(s)	719, 820
BanII	GRGCYC	2 cut(s)	294, 966
BauI	CACGAG	1 cut(s)	765
BbsI	GAAGAC	1 cut(s)	1292
Bbv12I	GWGCWC	1 cut(s)	294
BbvCI	CCTCAGC	1 cut(s)	1112
BbvI	GCAGC	2 cut(s)	695, 1054
BccI	CCATC	3 cut(s)	154, 494, 566
BciT130I	CCWGG	1 cut(s)	1268
BcoDI	GTCTC	1 cut(s)	95
BfaI	CTAG	1 cut(s)	90
BfuAI	ACCTGC	1 cut(s)	617
BglII	AGATCT	1 cut(s)	406
BisI	GCNGC	2 cut(s)	684, 1068
BlpI	GCTNAGC	1 cut(s)	1071
BlsI	GCNGC	2 cut(s)	685, 1069
Bme1390I	CCNGG	1 cut(s)	1268
Bme18I	GGWCC	1 cut(s)	550
BmeRI	GACNNNNNGTC	1 cut(s)	592
BmgBI	CACGTC	1 cut(s)	1090
BmgT120I	GGNCC	1 cut(s)	550
BmiI	GGNNCC	5 cut(s)	111, 446, 721, 750, 822
BmrFI	CCNGG	1 cut(s)	1268
BmrI	ACTGGG	1 cut(s)	114
BmsI	GCATC	2 cut(s)	217, 424
BmuI	ACTGGG	1 cut(s)	114
BoxI	GACNNNNGTC	1 cut(s)	767
BpiI	GAAGAC	1 cut(s)	1292
BplI	GAGNNNNNCTC	4 cut(s)	276, 308, 594, 626
BpmI	CTGGAG	2 cut(s)	303, 1289
Bpu10I	CCTNAGC	1 cut(s)	1112
Bpu1102I	GCTNAGC	1 cut(s)	1071
Bpu14I	TTCGAA	1 cut(s)	650
BpuEI	CTTGAG	1 cut(s)	395
BsaBI	GATNNNNATC	1 cut(s)	651
BsaHI	GRCGYC	1 cut(s)	393
BsaJI	CCNNGG	3 cut(s)	704, 970, 1227
Bsc4I	CCNNNNNNNGG	1 cut(s)	898
Bse1I	ACTGG	3 cut(s)	109, 286, 920
Bse21I	CCTNAGG	1 cut(s)	546
Bse8I	GATNNNNATC	1 cut(s)	651
BseBI	CCWGG	1 cut(s)	1268
BseDI	CCNNGG	3 cut(s)	704, 970, 1227
BseGI	GGATG	6 cut(s)	208, 439, 502, 862, 1022, 1279
BseJI	GATNNNNATC	1 cut(s)	651
BseLI	CCNNNNNNNGG	1 cut(s)	898
BseMII	CTCAG	4 cut(s)	90, 537, 678, 1103
BseNI	ACTGG	3 cut(s)	109, 286, 920
BseRI	GAGGAG	2 cut(s)	848, 1307
BseXI	GCAGC	2 cut(s)	695, 1054
BshFI	GGCC	1 cut(s)	1266
BshNI	GGYRCC	2 cut(s)	719, 820
BsiHKAI	GWGCWC	1 cut(s)	294
BslFI	GGGAC	2 cut(s)	684, 1235
BslI	CCNNNNNNNGG	1 cut(s)	898
BsmAI	GTCTC	1 cut(s)	95
BsmFI	GGGAC	2 cut(s)	684, 1235
BsmI	GAATGC	1 cut(s)	937
BsnI	GGCC	1 cut(s)	1266
Bsp119I	TTCGAA	1 cut(s)	650
Bsp1286I	GDGCHC	2 cut(s)	294, 966
Bsp143I	GATC	3 cut(s)	171, 406, 748
Bsp1720I	GCTNAGC	1 cut(s)	1071
BspACI	CCGC	2 cut(s)	178, 584
BspANI	GGCC	1 cut(s)	1266
BspCNI	CTCAG	4 cut(s)	91, 538, 679, 1104
BspLI	GGNNCC	5 cut(s)	111, 446, 721, 750, 822
BspMI	ACCTGC	1 cut(s)	617
BspPI	GGATC	2 cut(s)	743, 756
BspT104I	TTCGAA	1 cut(s)	650
BspT107I	GGYRCC	2 cut(s)	719, 820
BsrI	ACTGG	3 cut(s)	109, 286, 920
BssECI	CCNNGG	3 cut(s)	704, 970, 1227
BssMI	GATC	3 cut(s)	171, 406, 748
BssNI	GRCGYC	1 cut(s)	393
BssSI	CACGAG	1 cut(s)	765
BssT1I	CCWWGG	2 cut(s)	970, 1227
Bst2BI	CACGAG	1 cut(s)	765
Bst2UI	CCWGG	1 cut(s)	1268
Bst6I	CTCTTC	1 cut(s)	1194
BstACI	GRCGYC	1 cut(s)	393
BstBI	TTCGAA	1 cut(s)	650
BstC8I	GCNNGC	2 cut(s)	290, 624
BstDEI	CTNAG	5 cut(s)	99, 546, 687, 1071, 1112
BstDSI	CCRYGG	1 cut(s)	704
BstF5I	GGATG	6 cut(s)	208, 439, 502, 862, 1022, 1279
BstHHI	GCGC	1 cut(s)	476
BstKTI	GATC	3 cut(s)	174, 409, 751
BstMAI	GTCTC	1 cut(s)	95
BstMBI	GATC	3 cut(s)	171, 406, 748
BstMWI	GCNNNNNNNGC	2 cut(s)	205, 932
BstNI	CCWGG	1 cut(s)	1268
BstPAI	GACNNNNGTC	1 cut(s)	767
BstSCI	CCNGG	1 cut(s)	1266
BstV1I	GCAGC	2 cut(s)	695, 1054
BstV2I	GAAGAC	1 cut(s)	1292
BstX2I	RGATCY	2 cut(s)	406, 748
BstYI	RGATCY	2 cut(s)	406, 748
Bsu36I	CCTNAGG	1 cut(s)	546
BsuRI	GGCC	1 cut(s)	1266
BtgI	CCRYGG	1 cut(s)	704
BtrI	CACGTC	1 cut(s)	1090
BtsCI	GGATG	6 cut(s)	208, 439, 502, 862, 1022, 1279
BveI	ACCTGC	1 cut(s)	617
Cac8I	GCNNGC	2 cut(s)	290, 624
CfoI	GCGC	1 cut(s)	476
Cfr13I	GGNCC	1 cut(s)	550
CseI	GACGC	2 cut(s)	11, 382
Csp6I	GTAC	1 cut(s)	66
CviAII	CATG	4 cut(s)	597, 724, 893, 1195
CviQI	GTAC	1 cut(s)	66
DdeI	CTNAG	5 cut(s)	99, 546, 687, 1071, 1112
DpnI	GATC	3 cut(s)	173, 408, 750
DpnII	GATC	3 cut(s)	171, 406, 748
DraIII	CACNNNGTG	1 cut(s)	895
DriI	GACNNNNNGTC	1 cut(s)	592
Eam1104I	CTCTTC	1 cut(s)	1194
Eam1105I	GACNNNNNGTC	1 cut(s)	592
EarI	CTCTTC	1 cut(s)	1194
Ecl136II	GAGCTC	1 cut(s)	292
Eco130I	CCWWGG	2 cut(s)	970, 1227
Eco147I	AGGCCT	1 cut(s)	1266
Eco24I	GRGCYC	2 cut(s)	294, 966
Eco47I	GGWCC	1 cut(s)	550
Eco53kI	GAGCTC	1 cut(s)	292
Eco57I	CTGAAG	1 cut(s)	170
Eco81I	CCTNAGG	1 cut(s)	546
EcoICRI	GAGCTC	1 cut(s)	292
EcoO109I	RGGNCCY	1 cut(s)	550
EcoRII	CCWGG	1 cut(s)	1266
EcoT14I	CCWWGG	2 cut(s)	970, 1227
EcoT22I	ATGCAT	1 cut(s)	439
EcoT38I	GRGCYC	2 cut(s)	294, 966
ErhI	CCWWGG	2 cut(s)	970, 1227
FaeI	CATG	4 cut(s)	600, 727, 896, 1198
FaqI	GGGAC	2 cut(s)	684, 1235
FatI	CATG	4 cut(s)	596, 723, 892, 1194
FauI	CCCGC	1 cut(s)	171
FauNDI	CATATG	1 cut(s)	439
Fnu4HI	GCNGC	2 cut(s)	684, 1068
FokI	GGATG	6 cut(s)	195, 446, 509, 869, 1009, 1286
FriOI	GRGCYC	2 cut(s)	294, 966
Fsp4HI	GCNGC	2 cut(s)	684, 1068
FspBI	CTAG	1 cut(s)	90
GlaI	GCGC	1 cut(s)	475
GluI	GCNGC	2 cut(s)	684, 1068
GsuI	CTGGAG	2 cut(s)	303, 1289
HaeIII	GGCC	1 cut(s)	1266
HgaI	GACGC	2 cut(s)	11, 382
HhaI	GCGC	1 cut(s)	476
Hin1I	GRCGYC	1 cut(s)	393
Hin1II	CATG	4 cut(s)	600, 727, 896, 1198
Hin6I	GCGC	1 cut(s)	474
HinP1I	GCGC	1 cut(s)	474
HinfI	GANTC	5 cut(s)	4, 647, 652, 763, 881
HphI	GGTGA	1 cut(s)	71
Hpy166II	GTNNAC	3 cut(s)	310, 693, 1020
Hpy188I	TCNGA	5 cut(s)	100, 150, 460, 467, 886
Hpy188III	TCNNGA	3 cut(s)	131, 545, 805
Hpy8I	GTNNAC	3 cut(s)	310, 693, 1020
Hpy99I	CGWCG	1 cut(s)	27
HpyAV	CCTTC	5 cut(s)	42, 136, 802, 952, 1194
HpyCH4IV	ACGT	1 cut(s)	1089
HpyCH4V	TGCA	7 cut(s)	74, 368, 437, 626, 1093, 1181, 1303
HpyF10VI	GCNNNNNNNGC	2 cut(s)	205, 932
HpyF3I	CTNAG	5 cut(s)	99, 546, 687, 1071, 1112
HpySE526I	ACGT	1 cut(s)	1089
Hsp92I	GRCGYC	1 cut(s)	393
Hsp92II	CATG	4 cut(s)	600, 727, 896, 1198
HspAI	GCGC	1 cut(s)	474
Kzo9I	GATC	3 cut(s)	171, 406, 748
LmnI	GCTCC	2 cut(s)	109, 547
Lsp1109I	GCAGC	2 cut(s)	695, 1054
LweI	GCATC	2 cut(s)	217, 424
MaeI	CTAG	1 cut(s)	90
MaeII	ACGT	1 cut(s)	1089
MaeIII	GTNAC	5 cut(s)	277, 504, 629, 769, 895
MalI	GATC	3 cut(s)	173, 408, 750
MboI	GATC	3 cut(s)	171, 406, 748
MboII	GAAGA	7 cut(s)	341, 803, 923, 1073, 1149, 1181, 1297
MflI	RGATCY	2 cut(s)	406, 748
MhlI	GDGCHC	2 cut(s)	294, 966
MlyI	GAGTC	1 cut(s)	757
Mph1103I	ATGCAT	1 cut(s)	439
MseI	TTAA	9 cut(s)	204, 363, 450, 555, 731, 876, 944, 1002, 1152
MslI	CAYNNNNRTG	2 cut(s)	213, 1027
MspA1I	CMGCKG	1 cut(s)	686
MspR9I	CCNGG	1 cut(s)	1268
Mva1269I	GAATGC	1 cut(s)	937
MvaI	CCWGG	1 cut(s)	1268
MwoI	GCNNNNNNNGC	2 cut(s)	205, 932
NdeI	CATATG	1 cut(s)	439
NdeII	GATC	3 cut(s)	171, 406, 748
NlaIII	CATG	4 cut(s)	600, 727, 896, 1198
NlaIV	GGNNCC	5 cut(s)	111, 446, 721, 750, 822
NmeAIII	GCCGAG	1 cut(s)	659
NmuCI	GTSAC	2 cut(s)	769, 895
NsiI	ATGCAT	1 cut(s)	439
NspV	TTCGAA	1 cut(s)	650
PceI	AGGCCT	1 cut(s)	1266
PctI	GAATGC	1 cut(s)	937
PfeI	GAWTC	4 cut(s)	4, 647, 652, 881
PkrI	GCNGC	2 cut(s)	685, 1069
PleI	GAGTC	1 cut(s)	757
PpsI	GAGTC	1 cut(s)	757
PpuMI	RGGWCCY	1 cut(s)	550
PshAI	GACNNNNGTC	1 cut(s)	767
PshBI	ATTAAT	2 cut(s)	363, 731
Psp124BI	GAGCTC	1 cut(s)	294
Psp5II	RGGWCCY	1 cut(s)	550
Psp6I	CCWGG	1 cut(s)	1266
PspGI	CCWGG	1 cut(s)	1266
PspN4I	GGNNCC	5 cut(s)	111, 446, 721, 750, 822
PspPI	GGNCC	1 cut(s)	550
PspPPI	RGGWCCY	1 cut(s)	550
PsuI	RGATCY	2 cut(s)	406, 748
PvuII	CAGCTG	1 cut(s)	686
RsaI	GTAC	1 cut(s)	67
RsaNI	GTAC	1 cut(s)	66
RseI	CAYNNNNRTG	2 cut(s)	213, 1027
SacI	GAGCTC	1 cut(s)	294
SaqAI	TTAA	9 cut(s)	204, 363, 450, 555, 731, 876, 944, 1002, 1152
SatI	GCNGC	2 cut(s)	684, 1068
Sau3AI	GATC	3 cut(s)	171, 406, 748
Sau96I	GGNCC	1 cut(s)	550
SchI	GAGTC	1 cut(s)	757
ScrFI	CCNGG	1 cut(s)	1268
SduI	GDGCHC	2 cut(s)	294, 966
SfaNI	GCATC	2 cut(s)	217, 424
SfuI	TTCGAA	1 cut(s)	650
SinI	GGWCC	1 cut(s)	550
SmiMI	CAYNNNNRTG	2 cut(s)	213, 1027
SmlI	CTYRAG	1 cut(s)	410
SmoI	CTYRAG	1 cut(s)	410
SseBI	AGGCCT	1 cut(s)	1266
SsiI	CCGC	2 cut(s)	178, 584
SspMI	CTAG	1 cut(s)	90
SstI	GAGCTC	1 cut(s)	294
StuI	AGGCCT	1 cut(s)	1266
StyD4I	CCNGG	1 cut(s)	1266
StyI	CCWWGG	2 cut(s)	970, 1227
TaiI	ACGT	1 cut(s)	1092
TaqI	TCGA	4 cut(s)	650, 739, 956, 1200
TfiI	GAWTC	4 cut(s)	4, 647, 652, 881
Tru1I	TTAA	9 cut(s)	204, 363, 450, 555, 731, 876, 944, 1002, 1152
Tru9I	TTAA	9 cut(s)	204, 363, 450, 555, 731, 876, 944, 1002, 1152
TseFI	GTSAC	2 cut(s)	769, 895
TseI	GCWGC	2 cut(s)	683, 1067
Tsp45I	GTSAC	2 cut(s)	769, 895
TspGWI	ACGGA	3 cut(s)	355, 914, 1304
VpaK11BI	GGWCC	1 cut(s)	550
VspI	ATTAAT	2 cut(s)	363, 731
XapI	RAATTY	6 cut(s)	94, 126, 421, 741, 783, 976
XspI	CTAG	1 cut(s)	90
Zsp2I	ATGCAT	1 cut(s)	439

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.