Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000005

Orthogroup ID

OG0004420

Physical Location & Seq

Genomic Coordinates Reverse (-)

28881864 .. 28884962

Gene Structure 3099 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug05G0257300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1527 bp

ATGAGACTATGCATCGTCCTGTTTCTGATAACCAGACTCTTTACAAACTCTCAATCTCTTAGCAGGGCTGATTTCCCAGAAGGGTTTGTTTTCGGAACAGCTTCTTCGGCATACCAGTTCGAGGGAGCTGTAAATGAAGGAAACAAGGGAGATAGTGTATGGGATACTTTCACAAAAAAACCAGGGAAGATTTTAGATTTCAGCAATGCAGACACAGCGGTAGATCAATATCACAGATTTCAGAGTGATGTAGACTTGATGAAGGATATGGGAGTAAATGCATACAGATTCTCAATATCGTGGCCAAGAATTTTTCCAAATGGAACAGGAGCACCCAACTCAGAAGGAATAAGCTACTACAACAGCCTTATAGATTCTTTGCTTGCAAAAGGGATCCAGCCTTTTGTAACTCTATATCATTGGGATCTTCCGCAGATGCTTGAAGACAGATATGAAGGATGGTTGAGCACACAGATAGTAAAAGACTTTGAACATTATGCCTTCACCTGCTTCCAAGCGTTTGGAGACAGAGTTAAGCACTGGATCACCATCAACGAGCCTCATGGTTATGCACTCCAAAGTTATGACTTGGGAATACAAGCTCCCGGGCGATGTTCTATTCTCGGTCACTTGTTTTGTAAGAAAGGAAATTCATCCGTTGAACCATACATTGTTGCTCACAACATCCTCCTGTCTCATGCTGCAACTTATCATATGTACCAGAAATTTTTCAAGGAGAAACAAGTAGGTCAGGTTGGAATTGCACTGGATGCAAAATGGCATGAACCTCTATCGGAAAGTGATGAGGACATAGATGCAGCACATAGAGCGAATGAATTCACATTGGGATGGTTCCTTGATCCACTATTTTTTGGCGACTATCCTCTCTCAATGAAGAAGCTTGTGGGTAAGCGATTACCTGAGATTTCGCAGGAAGCATCAAAATTCTTGGTGGGATCTGTGGACTTTATCGGCATAAACCACTACACCACATTATATGCTCGGAATGACAGGACACGGATTCGAAAATTTATATTGCAGGATGCTACATCTGATGCTGCAGTCATTACAACACAATACAAAGGAGGAGTGGCGATTGGAGAAAAAGCAGCTTCTCGCTGGTTACGCATAGTCCCATGGGGCATCCGAAAGTTGGCAAGATACATCAAAGATAAGTACGGGAACCCCCCAGTGATGATAACAGAAAATGGTATGGATGATCCAAATAAACCTTTCATATCTATGGAAAAGGCTTTAAATGATGAGAAGAGGATACGATTTCACAGGGACTATCTCTCAAACCTATCAGCTGCTATAAGGGAAGACAAATGTGATGTTCGTGGTTATTTTGTGTGGTCATTACTTGACAACTGGGAATGGAACATGGGCTACACTGTCAGATTTGGGCTCTACTATGTCGATTACAAGAAGAACCTTACGAGGATTCCTAAATCTTCTGTTCAATGGTTTACAAGATTTCTCCAAGTTAACAATCATCAGAGTTTTGTGCAGACAAAGCACATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04068 ko04140 ko04150 ko04151 ko04152 ko04211 ko04213 ko04371 ko04530 ko04710 ko04714 ko04910 ko04920 ko04921 ko04922 ko04931 ko04932 ko05410 ko05418 map04068 map04140 map04150 map04151 map04152 map04211 map04213 map04371 map04530 map04710 map04714 map04910 map04920 map04921 map04922 map04931 map04932 map05410 map05418

Pfam Domains

Protein Families

NAF Pkinase

Protein Analysis

508

Amino Acids

58.5

Weight (kDa)

8.34

Isoelectric Point (pI)

28.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_1	PF00232	23 - 497	5.4e-158	Glycosyl hydrolase family 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004420)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26980 AT2G26980 AT2G26980 AT2G26980 AT5G21326
fragaria_vesca	FvH4_3g27610 FvH4_3g27610 FvH4_3g27610 FvH4_3g27610 FvH4_3g27610 FvH4_3g27610 FvH4_3g27610 FvH4_3g27610
malus_domestica	MD03G1163600.v1.1
prunus_persica	Prupe.6G156900_v2.0.a1 Prupe.6G156900_v2.0.a1 Prupe.6G156900_v2.0.a1 Prupe.6G156900_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0050061
rosa_laevigata	RLG00000034683
rosa_multiflora	Rmu_sc0000333.1_g000007
rosa_roxburghii	Rroxscaffold_1G00031070
rosa_rugosa	Rorug05G0257000 Rorug05G0257100 Rorug05G0257200.1 Rorug05G0257300 Rorug05G0257400
rosa_samantha	Rh5AG330200 Rh5BG340900 Rh5CG367500 Rh5DG353700
rosa_wichuraiana	Rw5G031160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	515
Acc36I	ACCTGC	1 cut(s)	515
AccI	GTMKAC	1 cut(s)	252
AciI	CCGC	2 cut(s)	218, 431
AclWI	GGATC	7 cut(s)	388, 401, 432, 551, 854, 964, 1214
AcoI	YGGCCR	1 cut(s)	302
AcsI	RAATTY	6 cut(s)	309, 649, 725, 836, 944, 1028
AfaI	GTAC	2 cut(s)	719, 1178
AfiI	CCNNNNNNNGG	2 cut(s)	121, 1153
AflIII	ACRYGT	1 cut(s)	1521
AgsI	TTSAA	5 cut(s)	443, 491, 662, 733, 1463
AjnI	CCWGG	1 cut(s)	181
AluBI	AGCT	7 cut(s)	101, 128, 354, 602, 901, 1112, 1310
AluI	AGCT	7 cut(s)	101, 128, 354, 602, 901, 1112, 1310
Alw21I	GWGCWC	2 cut(s)	334, 470
Alw26I	GTCTC	2 cut(s)	519, 699
AlwI	GGATC	7 cut(s)	388, 401, 432, 551, 854, 964, 1214
Ama87I	CYCGRG	1 cut(s)	605
AoxI	GGCC	1 cut(s)	302
ApeKI	GCWGC	5 cut(s)	701, 818, 1058, 1109, 1310
ApoI	RAATTY	6 cut(s)	309, 649, 725, 836, 944, 1028
Asp700I	GAANNNNTTC	3 cut(s)	100, 728, 836
AsuC2I	CCSGG	2 cut(s)	606, 607
AsuHPI	GGTGA	2 cut(s)	496, 538
AsuII	TTCGAA	1 cut(s)	1024
AvaI	CYCGRG	1 cut(s)	605
BalI	TGGCCA	1 cut(s)	304
BamHI	GGATCC	1 cut(s)	393
BanII	GRGCYC	1 cut(s)	1410
BbsI	GAAGAC	2 cut(s)	450, 1329
Bbv12I	GWGCWC	2 cut(s)	334, 470
BbvI	GCAGC	5 cut(s)	688, 830, 1045, 1121, 1297
BccI	CCATC	3 cut(s)	453, 557, 843
BciT130I	CCWGG	1 cut(s)	183
BciVI	GTATCC	2 cut(s)	157, 1266
BcnI	CCSGG	2 cut(s)	606, 607
BcoDI	GTCTC	2 cut(s)	519, 699
BfmI	CTRYAG	1 cut(s)	1059
BfuAI	ACCTGC	1 cut(s)	515
BfuI	GTATCC	2 cut(s)	157, 1266
BisI	GCNGC	5 cut(s)	702, 819, 1059, 1110, 1311
BlsI	GCNGC	5 cut(s)	703, 820, 1060, 1111, 1312
Bme1390I	CCNGG	3 cut(s)	183, 606, 607
BmeT110I	CYCGRG	1 cut(s)	605
BmiI	GGNNCC	3 cut(s)	395, 854, 1184
BmrFI	CCNGG	3 cut(s)	183, 606, 607
BmrI	ACTGGG	2 cut(s)	1184, 1381
BmsI	GCATC	8 cut(s)	21, 426, 760, 805, 947, 1033, 1045, 1152
BmuI	ACTGGG	2 cut(s)	1184, 1381
BpiI	GAAGAC	2 cut(s)	450, 1329
Bpu14I	TTCGAA	1 cut(s)	1024
BpuMI	CCSGG	2 cut(s)	606, 607
BsaBI	GATNNNNATC	2 cut(s)	228, 548
BsaJI	CCNNGG	3 cut(s)	182, 605, 1136
BsaXI	ACNNNNNCTCC	4 cut(s)	141, 171, 516, 546
Bsc4I	CCNNNNNNNGG	2 cut(s)	121, 1153
Bse1I	ACTGG	5 cut(s)	115, 545, 771, 1190, 1376
Bse3DI	GCAATG	1 cut(s)	211
Bse8I	GATNNNNATC	2 cut(s)	228, 548
BseBI	CCWGG	1 cut(s)	183
BseDI	CCNNGG	3 cut(s)	182, 605, 1136
BseGI	GGATG	8 cut(s)	464, 653, 684, 775, 854, 1048, 1143, 1222
BseJI	GATNNNNATC	2 cut(s)	228, 548
BseLI	CCNNNNNNNGG	2 cut(s)	121, 1153
BseMI	GCAATG	1 cut(s)	211
BseMII	CTCAG	2 cut(s)	354, 912
BseNI	ACTGG	5 cut(s)	115, 545, 771, 1190, 1376
BseRI	GAGGAG	1 cut(s)	1101
BseXI	GCAGC	5 cut(s)	688, 830, 1045, 1121, 1297
BshFI	GGCC	1 cut(s)	304
BsiHKAI	GWGCWC	2 cut(s)	334, 470
BsiHKCI	CYCGRG	1 cut(s)	605
BsiSI	CCGG	1 cut(s)	606
BslFI	GGGAC	2 cut(s)	1118, 1301
BslI	CCNNNNNNNGG	2 cut(s)	121, 1153
BsmAI	GTCTC	2 cut(s)	519, 699
BsmFI	GGGAC	2 cut(s)	1118, 1301
BsnI	GGCC	1 cut(s)	304
BsoBI	CYCGRG	1 cut(s)	605
Bsp119I	TTCGAA	1 cut(s)	1024
Bsp1286I	GDGCHC	3 cut(s)	334, 470, 1410
Bsp143I	GATC	7 cut(s)	223, 393, 424, 543, 859, 956, 1219
Bsp19I	CCATGG	1 cut(s)	1136
BspACI	CCGC	2 cut(s)	218, 431
BspANI	GGCC	1 cut(s)	304
BspCNI	CTCAG	2 cut(s)	353, 913
BspLI	GGNNCC	3 cut(s)	395, 854, 1184
BspMAI	CTGCAG	1 cut(s)	1063
BspMI	ACCTGC	1 cut(s)	515
BspPI	GGATC	7 cut(s)	388, 401, 432, 551, 854, 964, 1214
BspT104I	TTCGAA	1 cut(s)	1024
BsrDI	GCAATG	1 cut(s)	211
BsrI	ACTGG	5 cut(s)	115, 545, 771, 1190, 1376
BssECI	CCNNGG	3 cut(s)	182, 605, 1136
BssMI	GATC	7 cut(s)	223, 393, 424, 543, 859, 956, 1219
BssT1I	CCWWGG	1 cut(s)	1136
Bst2UI	CCWGG	1 cut(s)	183
Bst4CI	ACNGT	1 cut(s)	1396
Bst6I	CTCTTC	1 cut(s)	1262
BstAPI	GCANNNNNTGC	1 cut(s)	770
BstBI	TTCGAA	1 cut(s)	1024
BstC8I	GCNNGC	1 cut(s)	384
BstDEI	CTNAG	3 cut(s)	59, 340, 921
BstDSI	CCRYGG	1 cut(s)	1136
BstF5I	GGATG	8 cut(s)	464, 653, 684, 775, 854, 1048, 1143, 1222
BstKTI	GATC	7 cut(s)	226, 396, 427, 546, 862, 959, 1222
BstMAI	GTCTC	2 cut(s)	519, 699
BstMBI	GATC	7 cut(s)	223, 393, 424, 543, 859, 956, 1219
BstMWI	GCNNNNNNNGC	6 cut(s)	107, 215, 770, 827, 1125, 1516
BstNI	CCWGG	1 cut(s)	183
BstNSI	RCATGY	1 cut(s)	1525
BstSCI	CCNGG	3 cut(s)	181, 604, 605
BstSFI	CTRYAG	1 cut(s)	1059
BstV1I	GCAGC	5 cut(s)	688, 830, 1045, 1121, 1297
BstV2I	GAAGAC	2 cut(s)	450, 1329
BstX2I	RGATCY	3 cut(s)	393, 424, 956
BstXI	CCANNNNNNTGG	1 cut(s)	521
BstYI	RGATCY	3 cut(s)	393, 424, 956
BsuI	GTATCC	2 cut(s)	157, 1266
BsuRI	GGCC	1 cut(s)	304
BtgI	CCRYGG	1 cut(s)	1136
BtgZI	GCGATG	1 cut(s)	625
BtsCI	GGATG	8 cut(s)	464, 653, 684, 775, 854, 1048, 1143, 1222
BtsIMutI	CAGTG	4 cut(s)	538, 764, 1197, 1392
BveI	ACCTGC	1 cut(s)	515
Cac8I	GCNNGC	1 cut(s)	384
Cfr9I	CCCGGG	1 cut(s)	605
Csp6I	GTAC	2 cut(s)	718, 1177
CviAII	CATG	6 cut(s)	563, 698, 782, 1137, 1384, 1522
CviQI	GTAC	2 cut(s)	718, 1177
DdeI	CTNAG	3 cut(s)	59, 340, 921
DpnI	GATC	7 cut(s)	225, 395, 426, 545, 861, 958, 1221
DpnII	GATC	7 cut(s)	223, 393, 424, 543, 859, 956, 1219
DraI	TTTAAA	1 cut(s)	1257
EaeI	YGGCCR	1 cut(s)	302
Eam1104I	CTCTTC	1 cut(s)	1262
EarI	CTCTTC	1 cut(s)	1262
Eco130I	CCWWGG	1 cut(s)	1136
Eco24I	GRGCYC	1 cut(s)	1410
Eco88I	CYCGRG	1 cut(s)	605
EcoRI	GAATTC	1 cut(s)	836
EcoRII	CCWGG	1 cut(s)	181
EcoT14I	CCWWGG	1 cut(s)	1136
EcoT22I	ATGCAT	2 cut(s)	14, 283
EcoT38I	GRGCYC	1 cut(s)	1410
ErhI	CCWWGG	1 cut(s)	1136
FaeI	CATG	6 cut(s)	566, 701, 785, 1140, 1387, 1525
FaqI	GGGAC	2 cut(s)	1118, 1301
FatI	CATG	6 cut(s)	562, 697, 781, 1136, 1383, 1521
FauNDI	CATATG	1 cut(s)	714
FblI	GTMKAC	1 cut(s)	252
Fnu4HI	GCNGC	5 cut(s)	702, 819, 1059, 1110, 1311
FokI	GGATG	8 cut(s)	471, 640, 671, 782, 861, 1055, 1130, 1229
FriOI	GRGCYC	1 cut(s)	1410
Fsp4HI	GCNGC	5 cut(s)	702, 819, 1059, 1110, 1311
GluI	GCNGC	5 cut(s)	702, 819, 1059, 1110, 1311
HaeIII	GGCC	1 cut(s)	304
HapII	CCGG	1 cut(s)	606
Hin1II	CATG	6 cut(s)	566, 701, 785, 1140, 1387, 1525
HincII	GTYRAC	1 cut(s)	1489
HindII	GTYRAC	1 cut(s)	1489
HindIII	AAGCTT	1 cut(s)	899
HinfI	GANTC	5 cut(s)	36, 288, 374, 1021, 1444
HpaI	GTTAAC	1 cut(s)	1489
HpaII	CCGG	1 cut(s)	606
HphI	GGTGA	2 cut(s)	496, 538
Hpy166II	GTNNAC	4 cut(s)	253, 964, 1470, 1489
Hpy8I	GTNNAC	4 cut(s)	253, 964, 1470, 1489
HpyAV	CCTTC	6 cut(s)	74, 131, 256, 338, 449, 511
HpyCH4III	ACNGT	1 cut(s)	1396
HpyF10VI	GCNNNNNNNGC	6 cut(s)	107, 215, 770, 827, 1125, 1516
HpyF3I	CTNAG	3 cut(s)	59, 340, 921
Hsp92II	CATG	6 cut(s)	566, 701, 785, 1140, 1387, 1525
KspAI	GTTAAC	1 cut(s)	1489
Kzo9I	GATC	7 cut(s)	223, 393, 424, 543, 859, 956, 1219
LmnI	GCTCC	3 cut(s)	125, 329, 607
Lsp1109I	GCAGC	5 cut(s)	688, 830, 1045, 1121, 1297
LweI	GCATC	8 cut(s)	21, 426, 760, 805, 947, 1033, 1045, 1152
MaeIII	GTNAC	3 cut(s)	406, 626, 1122
MalI	GATC	7 cut(s)	225, 395, 426, 545, 861, 958, 1221
MboI	GATC	7 cut(s)	223, 393, 424, 543, 859, 956, 1219
MboII	GAAGA	9 cut(s)	96, 199, 419, 455, 907, 1279, 1334, 1441, 1446
MflI	RGATCY	3 cut(s)	393, 424, 956
MhlI	GDGCHC	3 cut(s)	334, 470, 1410
MlsI	TGGCCA	1 cut(s)	304
MluCI	AATT	7 cut(s)	309, 649, 725, 759, 836, 944, 1028
MluNI	TGGCCA	1 cut(s)	304
MlyI	GAGTC	1 cut(s)	30
MmeI	TCCRAC	1 cut(s)	736
MnlI	CCTC	9 cut(s)	115, 570, 698, 798, 799, 894, 1079, 1263, 1434
Mox20I	TGGCCA	1 cut(s)	304
Mph1103I	ATGCAT	2 cut(s)	14, 283
MroXI	GAANNNNTTC	3 cut(s)	100, 728, 836
MscI	TGGCCA	1 cut(s)	304
MseI	TTAA	3 cut(s)	534, 1256, 1488
MslI	CAYNNNNRTG	3 cut(s)	567, 847, 1241
Msp20I	TGGCCA	1 cut(s)	304
MspA1I	CMGCKG	2 cut(s)	218, 1310
MspI	CCGG	1 cut(s)	606
MspR9I	CCNGG	3 cut(s)	183, 606, 607
MvaI	CCWGG	1 cut(s)	183
MwoI	GCNNNNNNNGC	6 cut(s)	107, 215, 770, 827, 1125, 1516
NciI	CCSGG	2 cut(s)	606, 607
NcoI	CCATGG	1 cut(s)	1136
NdeI	CATATG	1 cut(s)	714
NdeII	GATC	7 cut(s)	223, 393, 424, 543, 859, 956, 1219
NlaIII	CATG	6 cut(s)	566, 701, 785, 1140, 1387, 1525
NlaIV	GGNNCC	3 cut(s)	395, 854, 1184
NmuCI	GTSAC	1 cut(s)	626
NsiI	ATGCAT	2 cut(s)	14, 283
NspI	RCATGY	1 cut(s)	1525
NspV	TTCGAA	1 cut(s)	1024
PaqCI	CACCTGC	1 cut(s)	515
PciI	ACATGT	1 cut(s)	1521
PdmI	GAANNNNTTC	3 cut(s)	100, 728, 836
PfeI	GAWTC	4 cut(s)	288, 374, 1021, 1444
PkrI	GCNGC	5 cut(s)	703, 820, 1060, 1111, 1312
PleI	GAGTC	1 cut(s)	30
PpsI	GAGTC	1 cut(s)	30
PscI	ACATGT	1 cut(s)	1521
Psp6I	CCWGG	1 cut(s)	181
PspGI	CCWGG	1 cut(s)	181
PspN4I	GGNNCC	3 cut(s)	395, 854, 1184
PsrI	GAACNNNNNNTAC	2 cut(s)	1373, 1405
PstI	CTGCAG	1 cut(s)	1063
PsuI	RGATCY	3 cut(s)	393, 424, 956
PvuII	CAGCTG	1 cut(s)	1310
RsaI	GTAC	2 cut(s)	719, 1178
RsaNI	GTAC	2 cut(s)	718, 1177
RseI	CAYNNNNRTG	3 cut(s)	567, 847, 1241
SaqAI	TTAA	3 cut(s)	534, 1256, 1488
SatI	GCNGC	5 cut(s)	702, 819, 1059, 1110, 1311
Sau3AI	GATC	7 cut(s)	223, 393, 424, 543, 859, 956, 1219
SchI	GAGTC	1 cut(s)	30
ScrFI	CCNGG	3 cut(s)	183, 606, 607
SduI	GDGCHC	3 cut(s)	334, 470, 1410
SfaNI	GCATC	8 cut(s)	21, 426, 760, 805, 947, 1033, 1045, 1152
SfcI	CTRYAG	1 cut(s)	1059
SfuI	TTCGAA	1 cut(s)	1024
SmaI	CCCGGG	1 cut(s)	607
SmiMI	CAYNNNNRTG	3 cut(s)	567, 847, 1241
Sse9I	AATT	7 cut(s)	309, 649, 725, 759, 836, 944, 1028
SsiI	CCGC	2 cut(s)	218, 431
StyD4I	CCNGG	3 cut(s)	181, 604, 605
StyI	CCWWGG	1 cut(s)	1136
TaaI	ACNGT	1 cut(s)	1396
TaqI	TCGA	3 cut(s)	120, 1024, 1419
TaqII	GACCGA	1 cut(s)	614
TasI	AATT	7 cut(s)	309, 649, 725, 759, 836, 944, 1028
TfiI	GAWTC	4 cut(s)	288, 374, 1021, 1444
Tru1I	TTAA	3 cut(s)	534, 1256, 1488
Tru9I	TTAA	3 cut(s)	534, 1256, 1488
TscAI	CASTG	4 cut(s)	545, 771, 1197, 1399
TseFI	GTSAC	1 cut(s)	626
TseI	GCWGC	5 cut(s)	701, 818, 1058, 1109, 1310
Tsp45I	GTSAC	1 cut(s)	626
TspDTI	ATGAA	8 cut(s)	150, 275, 468, 642, 798, 849, 908, 1225
TspGWI	ACGGA	2 cut(s)	646, 1033
TspMI	CCCGGG	1 cut(s)	605
TspRI	CASTG	4 cut(s)	545, 771, 1197, 1399
XapI	RAATTY	6 cut(s)	309, 649, 725, 836, 944, 1028
XceI	RCATGY	1 cut(s)	1525
XmaI	CCCGGG	1 cut(s)	605
XmiI	GTMKAC	1 cut(s)	252
XmnI	GAANNNNTTC	3 cut(s)	100, 728, 836
Zsp2I	ATGCAT	2 cut(s)	14, 283

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.