MD03G1169700.v1.1

Nuclear cap-binding protein subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0007238
Physical Location & Seq
Forward (+)
22970489 .. 22991203
20715 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1169700.v1.1.491

Sequence Viewer

Length: 2601 bp
ATGAGCAGTTGGAAGAGTCTTCTTCTTCGAATTGGCGAGAAATCTCCCGACTATGGCACCTCCTCCGACGTCAAAGAACACATTGAAACTTGCTATGGCGTGCTTCGCCGAGAATTGGAGCACTCTCCTGACGACGTTTCGCAATACCTTATCCAGTGTGCTGAACAGTTGCCTCACAAGAATCCTCTATATGGGACAGTGATTGGCTTGTTAAATTTGGAGAACGAGGAATTTATTAGGAAAGTAGTTAAGAACACTCAAACTAATTTCCAGGATGCTTTAGATTCCGGAAATTGTAACAAGATTCGTATTTTGATGAGGTTCCTGACTGTTATGATGTGCAGTAAAGTTATCCATCCCAGTTCTCTTGTGGTTGTGTTTGAAACATTATTATCATCAGCTGCAACAACAGTTGATGAGGAGAAAGGCAATCCGTCCTGGCAATCGCGAGCTGACTTTTACATAACATGCATTTTATCTTGTCTCCCCTGGGGAGGAGCGGAACTCATTGAGCAAGTTCCTGAGGAAATTGAGAGAGTTATGGTCGGTGTTGAAGCTTATTTGAGCATAAGAAAACGTGCTTCTGACACTGGGTTGTCCTTTTTTGAGGATGATGATGAGAATGCGAGAGAACCTAGCGATAAGGATTTCTTGGAAGATTTGTGGGGCCGAATACAAGTTTTATCTAGCAATGGATGGAAACTTGATAGTGTTCCGAGACCTCACCTATCATTTGAAGCACAGCTAGTTGCTGGAAAGTCTCATGAGTTTGGACCCATCAGCTGTCCTGACCAACCTGCTATGCCTTCAACAATGTCTAATATAACTTATGGTAAACAAAAGCATGATGCAGAGTTTATCTATCCGCAAAGGATACGAAGGCTGAATATATTTCCTGAAAGTAAAACTGAGGTAGATGTGCAACCCATGGATCGCTTCATCGTGGAAGAGTATCTACTAGATGTGCTATTTTACTTAAATGGCTGTCGAAAGGAATGTGCTTCATATATGGCTGGCCTACCTGTGCCCTTTAGATATGAGTATCTTATGGCAGAGACAATTTTCTCCCAGTTGCTTATGCTACCACAACCCCCTTTCAGGACAATATATTACACGCTAGTTATTATTGACCTCTGTAAGGCACTTCCTGGAGCCTTTCCTGCAGTAGTTGCTGGTGCTGTACGAGCTCTTTTTGACAAAATGGCTGATTTAGATATGGAATGTCGAACACGACTCATCCTTTGGTTTTCACACCACTTATCAAACTTTCAGTTCATCTGGCCATGGGAAGAGTGGGCTCATGTCTTAGACCTTCCAAAATGGGCCCCACAACGTGTTTTTGTTCAGGAGGTTCTGGAGAGAGAAGTCCGTTTATCTTACTGGGATAAAGTGAAGCAGAGTATTGAGCATGCCCCTGGTCTAGAAGAGTTGCTTCCTCCCAAGGGAGCACCAAATTTCAAATTTAGTGTTCAAGAAACCAGTGAAGAAGATGGGCTACATGCACTTTCTTCTGACCTCAGAAACATGGTGAAAGCTAGGGCATCTGCCCGTGAAATGATAGTATGGATAGAGGAAAGTGTGTTTCCTGTTCATGGGATGGAAGTTACCCTCAATGTGGTTGTGCAAACTCTCCTCGATATTGGATCTAAAAGTTTCACTCATTTGATTACTGTATTGGAGAGATATGGCCAAGTCATTGCAAAACTATGTGCTGACCAGGATAAGCAGGTCATGCTGATAAATGAGATTGATTCTTACTGGAGGAATAATTCTCAAATGACTGCTATTGCAATAGATAGGATGATGGGCTACCGAATTTTGTCTAATCTGGCCATTGTCAGATGGGTCTTTTTACCAGCAAACATTGAGCAGTTTCACATATCAGATCGTCCATGGGAGATACTAAGAAATGCAGTTAGCAAGACATATAACCGTGTATGTGATCTAAGGAAGGAGATTTCTTCTTTCAAAAAGAGTGTTGTATCAGCTGAAGAAGCTGCAGCAACGGTGAAAGCAGAGTTAGTAGCTTCTGAATCAAAGCTTTCACTCATGGATGGCGAACCTGTTACTGGGGAAAATCCTGCGTGGCTGAAGCGTTTGAAATCACGTGCTGAAAAGGCTAAGGAGGAGGTGGTGTCTGTACGTGAATCTTTAGAGGCTAAGGAGGCTCTTCTATCCCGATCCCTTGATGAATTTGAGGCATTATTCTTCTCCATGTACAAAAGCTTCCTTAATTTGTTAACGGAACGGCTGCCAGAAGCATCCAAATGTGTCACATTGCAGGGATTAAAGGGGATTCATGCAGATTCTATGGCAATTGACCTTGAAGAATCATCCGCAATGGATGTAGACAATGAGAATGGAATACCTAAGAAAAGTGAACTGAGTGGTGGGAGGACAAGTAATGTTTATAATGTAGGTGAAAAAGAGCAATGGTGTCTTTCAACTTTAGGCTATCTCAAGGCGTTCTCGAGGCAATATGCTTCCGAGATTTGGCCTCACATGGAGAAGTTGGATGCTGAGGTTCTAACCGAAGATGTGCATCCTCTTATTCGGAAATCAGTTTATTGTGGTCTTCGTCGAGAAACTGATGGGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000184 GO:0000339 GO:0000375 GO:0000377 GO:0000394 GO:0000398 GO:0000956 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005845 GO:0005846 GO:0006139 GO:0006396 GO:0006397 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0008380 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009892 GO:0009987 GO:0010033 GO:0010228 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0019222 GO:0019439 GO:0022414 GO:0031047 GO:0031050 GO:0031053 GO:0031503 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033993 GO:0034470 GO:0034518 GO:0034613 GO:0034641 GO:0034655 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044464 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048519 GO:0048571 GO:0048573 GO:0048574 GO:0048608 GO:0048731 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070727 GO:0070887 GO:0070918 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0097159 GO:0097305 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901698 GO:1901699 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 map03013 map03015 map03040
Pfam Domains
Protein Families
MIF4G MIF4G_like MIF4G_like_2

Protein Analysis

867

Amino Acids

98.67

Weight (kDa)

5.24

Isoelectric Point (pI)

47.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF4G PF02854 20 - 224 2.8e-29 MIF4G domain
MIF4G_like PF09088 341 - 468 1.1e-37 MIF4G like
MIF4G_like_2 PF09090 486 - 828 5.2e-32 MIF4G like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2412
AatII GACGTC 1 cut(s) 72
Acc36I ACCTGC 2 cut(s) 805, 1717
AccB1I GGYRCC 1 cut(s) 56
AccBSI CCGCTC 1 cut(s) 500
AccI GTMKAC 1 cut(s) 2349
AccII CGCG 1 cut(s) 448
AccIII TCCGGA 1 cut(s) 287
AciI CCGC 3 cut(s) 500, 866, 2337
AclWI GGATC 3 cut(s) 939, 1651, 2175
AcoI YGGCCR 3 cut(s) 1280, 1687, 1830
AcsI RAATTY 6 cut(s) 214, 230, 1453, 1460, 1815, 2192
AcuI CTGAAG 2 cut(s) 2010, 2111
AcvI CACGTG 1 cut(s) 2108
AcyI GRCGYC 1 cut(s) 69
AdeI CACNNNGTG 1 cut(s) 1334
AfaI GTAC 3 cut(s) 1182, 2142, 2219
AflIII ACRYGT 1 cut(s) 1333
AjnI CCWGG 6 cut(s) 270, 437, 488, 1147, 1414, 1716
Alw21I GWGCWC 3 cut(s) 123, 1189, 1450
Alw26I GTCTC 4 cut(s) 488, 712, 765, 1049
AlwI GGATC 3 cut(s) 939, 1651, 2175
Ama87I CYCGRG 1 cut(s) 2470
Aor13HI TCCGGA 1 cut(s) 287
AoxI GGCC 7 cut(s) 667, 1015, 1280, 1323, 1687, 1830, 2495
ApaI GGGCCC 1 cut(s) 1327
ApeKI GCWGC 4 cut(s) 401, 1997, 2000, 2251
ApoI RAATTY 6 cut(s) 214, 230, 1453, 1460, 1815, 2192
ArsI GACNNNNNNTTYG 2 cut(s) 1224, 1256
AspS9I GGNCC 4 cut(s) 667, 773, 1323, 1324
AsuHPI GGTGA 4 cut(s) 716, 1540, 2020, 2432
AsuII TTCGAA 1 cut(s) 28
AvaI CYCGRG 1 cut(s) 2470
AvaII GGWCC 1 cut(s) 773
AxyI CCTNAGG 1 cut(s) 522
BaeGI GKGCMC 2 cut(s) 1029, 1327
BalI TGGCCA 3 cut(s) 1282, 1689, 1832
BanI GGYRCC 1 cut(s) 56
BanII GRGCYC 3 cut(s) 1189, 1300, 1327
BarI GAAGNNNNNNTAC 6 cut(s) 939, 971, 1479, 1511, 2210, 2242
BbrPI CACGTG 1 cut(s) 2108
BbsI GAAGAC 2 cut(s) 11, 2567
Bbv12I GWGCWC 3 cut(s) 123, 1189, 1450
BbvCI CCTCAGC 1 cut(s) 2520
BbvI GCAGC 4 cut(s) 388, 1984, 2012, 2238
BccI CCATC 9 cut(s) 363, 690, 785, 1484, 1591, 1798, 1836, 2048, 2585
BceAI ACGGC 1 cut(s) 2264
BciT130I CCWGG 6 cut(s) 272, 439, 490, 1149, 1416, 1718
BciVI GTATCC 1 cut(s) 867
BcoDI GTCTC 4 cut(s) 488, 712, 765, 1049
BfaI CTAG 7 cut(s) 636, 687, 746, 959, 1118, 1421, 1536
BfmI CTRYAG 2 cut(s) 1161, 1998
BfuAI ACCTGC 2 cut(s) 805, 1717
BfuI GTATCC 1 cut(s) 867
BisI GCNGC 4 cut(s) 402, 1998, 2001, 2252
BlsI GCNGC 4 cut(s) 403, 1999, 2002, 2253
Bme1390I CCNGG 6 cut(s) 272, 439, 490, 1149, 1416, 1718
Bme18I GGWCC 1 cut(s) 773
BmeT110I CYCGRG 1 cut(s) 2470
BmgT120I GGNCC 4 cut(s) 667, 773, 1323, 1324
BmiI GGNNCC 7 cut(s) 58, 323, 668, 775, 1153, 1325, 1326
BmrFI CCNGG 6 cut(s) 272, 439, 490, 1149, 1416, 1718
BmrI ACTGGG 5 cut(s) 354, 600, 1063, 1390, 2079
BmsI GCATC 6 cut(s) 265, 838, 1550, 2270, 2506, 2551
BmuI ACTGGG 5 cut(s) 354, 600, 1063, 1390, 2079
BpiI GAAGAC 2 cut(s) 11, 2567
BplI GAGNNNNNCTC 2 cut(s) 489, 521
BpmI CTGGAG 3 cut(s) 1170, 1376, 1780
Bpu10I CCTNAGC 3 cut(s) 2121, 2160, 2520
Bpu14I TTCGAA 1 cut(s) 28
BpuEI CTTGAG 1 cut(s) 2444
BsaAI YACGTR 2 cut(s) 2108, 2144
BsaBI GATNNNNATC 1 cut(s) 2541
BsaHI GRCGYC 1 cut(s) 69
BsaI GGTCTC 1 cut(s) 712
BsaJI CCNNGG 7 cut(s) 488, 489, 927, 1283, 1414, 1440, 1892
BsaWI WCCGGW 1 cut(s) 287
BsaXI ACNNNNNCTCC 4 cut(s) 2120, 2150, 2386, 2416
Bse1I ACTGG 8 cut(s) 154, 360, 595, 1069, 1385, 1479, 1763, 2074
Bse21I CCTNAGG 1 cut(s) 522
Bse3DI GCAATG 5 cut(s) 697, 1695, 2276, 2346, 2438
Bse8I GATNNNNATC 1 cut(s) 2541
BseAI TCCGGA 1 cut(s) 287
BseBI CCWGG 6 cut(s) 272, 439, 490, 1149, 1416, 1718
BseDI CCNNGG 7 cut(s) 488, 489, 927, 1283, 1414, 1440, 1892
BseJI GATNNNNATC 1 cut(s) 2541
BseMI GCAATG 5 cut(s) 697, 1695, 2276, 2346, 2438
BseMII CTCAG 5 cut(s) 513, 900, 1531, 2375, 2511
BseNI ACTGG 8 cut(s) 154, 360, 595, 1069, 1385, 1479, 1763, 2074
BseRI GAGGAG 5 cut(s) 52, 434, 510, 1622, 2141
BseSI GKGCMC 2 cut(s) 1029, 1327
BseXI GCAGC 4 cut(s) 388, 1984, 2012, 2238
BsgI GTGCAG 1 cut(s) 361
Bsh1236I CGCG 1 cut(s) 448
BshFI GGCC 7 cut(s) 669, 1017, 1282, 1325, 1689, 1832, 2497
BshNI GGYRCC 1 cut(s) 56
BsiHKAI GWGCWC 3 cut(s) 123, 1189, 1450
BsiHKCI CYCGRG 1 cut(s) 2470
BsiSI CCGG 1 cut(s) 288
BslFI GGGAC 1 cut(s) 208
BsmAI GTCTC 4 cut(s) 488, 712, 765, 1049
BsmFI GGGAC 1 cut(s) 208
BsmI GAATGC 1 cut(s) 628
BsnI GGCC 7 cut(s) 669, 1017, 1282, 1325, 1689, 1832, 2497
Bso31I GGTCTC 1 cut(s) 712
BsoBI CYCGRG 1 cut(s) 2470
Bsp119I TTCGAA 1 cut(s) 28
Bsp120I GGGCCC 1 cut(s) 1323
Bsp1286I GDGCHC 6 cut(s) 123, 1029, 1189, 1300, 1327, 1450
Bsp13I TCCGGA 1 cut(s) 287
Bsp1407I TGTACA 1 cut(s) 2217
Bsp143I GATC 5 cut(s) 931, 1643, 1885, 1942, 2180
Bsp19I CCATGG 3 cut(s) 927, 1283, 1892
Bsp68I TCGCGA 1 cut(s) 448
BspACI CCGC 3 cut(s) 500, 866, 2337
BspANI GGCC 7 cut(s) 669, 1017, 1282, 1325, 1689, 1832, 2497
BspCNI CTCAG 5 cut(s) 514, 901, 1530, 2376, 2512
BspEI TCCGGA 1 cut(s) 287
BspFNI CGCG 1 cut(s) 448
BspHI TCATGA 1 cut(s) 763
BspLI GGNNCC 7 cut(s) 58, 323, 668, 775, 1153, 1325, 1326
BspMAI CTGCAG 2 cut(s) 1165, 2002
BspMI ACCTGC 2 cut(s) 805, 1717
BspPI GGATC 3 cut(s) 939, 1651, 2175
BspQI GCTCTTC 1 cut(s) 2175
BspT104I TTCGAA 1 cut(s) 28
BspT107I GGYRCC 1 cut(s) 56
BspTNI GGTCTC 1 cut(s) 712
BsrBI CCGCTC 1 cut(s) 500
BsrDI GCAATG 5 cut(s) 697, 1695, 2276, 2346, 2438
BsrGI TGTACA 1 cut(s) 2217
BsrI ACTGG 8 cut(s) 154, 360, 595, 1069, 1385, 1479, 1763, 2074
BssECI CCNNGG 7 cut(s) 488, 489, 927, 1283, 1414, 1440, 1892
BssMI GATC 5 cut(s) 931, 1643, 1885, 1942, 2180
BssNI GRCGYC 1 cut(s) 69
BssT1I CCWWGG 4 cut(s) 927, 1283, 1440, 1892
Bst2UI CCWGG 6 cut(s) 272, 439, 490, 1149, 1416, 1718
Bst4CI ACNGT 7 cut(s) 168, 199, 331, 412, 1672, 1934, 2008
Bst6I CTCTTC 5 cut(s) 8, 942, 1284, 1419, 2175
BstACI GRCGYC 1 cut(s) 69
BstAPI GCANNNNNTGC 2 cut(s) 1169, 1732
BstAUI TGTACA 1 cut(s) 2217
BstBAI YACGTR 2 cut(s) 2108, 2144
BstBI TTCGAA 1 cut(s) 28
BstC8I GCNNGC 4 cut(s) 101, 450, 1015, 1410
BstDSI CCRYGG 3 cut(s) 927, 1283, 1892
BstENI CCTNNNNNAGG 1 cut(s) 1136
BstFNI CGCG 1 cut(s) 448
BstKTI GATC 5 cut(s) 934, 1646, 1888, 1945, 2183
BstMAI GTCTC 4 cut(s) 488, 712, 765, 1049
BstMBI GATC 5 cut(s) 931, 1643, 1885, 1942, 2180
BstMWI GCNNNNNNNGC 8 cut(s) 105, 1160, 1169, 1184, 1732, 1994, 2117, 2165
BstNI CCWGG 6 cut(s) 272, 439, 490, 1149, 1416, 1718
BstNSI RCATGY 3 cut(s) 471, 1412, 1502
BstSCI CCNGG 6 cut(s) 270, 437, 488, 1147, 1414, 1716
BstSFI CTRYAG 2 cut(s) 1161, 1998
BstSLI GKGCMC 2 cut(s) 1029, 1327
BstUI CGCG 1 cut(s) 448
BstV1I GCAGC 4 cut(s) 388, 1984, 2012, 2238
BstV2I GAAGAC 2 cut(s) 11, 2567
BstX2I RGATCY 1 cut(s) 1643
BstYI RGATCY 1 cut(s) 1643
Bsu36I CCTNAGG 1 cut(s) 522
BsuI GTATCC 1 cut(s) 867
BsuRI GGCC 7 cut(s) 669, 1017, 1282, 1325, 1689, 1832, 2497
BtgI CCRYGG 3 cut(s) 927, 1283, 1892
BtsIMutI CAGTG 4 cut(s) 161, 204, 588, 1486
BtuMI TCGCGA 1 cut(s) 448
BveI ACCTGC 2 cut(s) 805, 1717
Cac8I GCNNGC 4 cut(s) 101, 450, 1015, 1410
CciI TCATGA 1 cut(s) 763
Cfr13I GGNCC 4 cut(s) 667, 773, 1323, 1324
Csp6I GTAC 3 cut(s) 1181, 2141, 2218
CviQI GTAC 3 cut(s) 1181, 2141, 2218
DpnI GATC 5 cut(s) 933, 1645, 1887, 1944, 2182
DpnII GATC 5 cut(s) 931, 1643, 1885, 1942, 2180
DraIII CACNNNGTG 1 cut(s) 1334
EaeI YGGCCR 3 cut(s) 1280, 1687, 1830
Eam1104I CTCTTC 5 cut(s) 8, 942, 1284, 1419, 2175
EarI CTCTTC 5 cut(s) 8, 942, 1284, 1419, 2175
Ecl136II GAGCTC 1 cut(s) 1187
Eco130I CCWWGG 4 cut(s) 927, 1283, 1440, 1892
Eco24I GRGCYC 3 cut(s) 1189, 1300, 1327
Eco31I GGTCTC 1 cut(s) 712
Eco47I GGWCC 1 cut(s) 773
Eco53kI GAGCTC 1 cut(s) 1187
Eco57I CTGAAG 2 cut(s) 2010, 2111
Eco72I CACGTG 1 cut(s) 2108
Eco81I CCTNAGG 1 cut(s) 522
Eco88I CYCGRG 1 cut(s) 2470
EcoICRI GAGCTC 1 cut(s) 1187
EcoNI CCTNNNNNAGG 1 cut(s) 1136
EcoO109I RGGNCCY 1 cut(s) 1324
EcoRII CCWGG 6 cut(s) 270, 437, 488, 1147, 1414, 1716
EcoT14I CCWWGG 4 cut(s) 927, 1283, 1440, 1892
EcoT22I ATGCAT 1 cut(s) 473
EcoT38I GRGCYC 3 cut(s) 1189, 1300, 1327
ErhI CCWWGG 4 cut(s) 927, 1283, 1440, 1892
FalI AAGNNNNNCTT 4 cut(s) 635, 667, 1416, 1448
FaqI GGGAC 1 cut(s) 208
FblI GTMKAC 1 cut(s) 2349
Fnu4HI GCNGC 4 cut(s) 402, 1998, 2001, 2252
FriOI GRGCYC 3 cut(s) 1189, 1300, 1327
Fsp4HI GCNGC 4 cut(s) 402, 1998, 2001, 2252
FspBI CTAG 7 cut(s) 636, 687, 746, 959, 1118, 1421, 1536
GluI GCNGC 4 cut(s) 402, 1998, 2001, 2252
GsuI CTGGAG 3 cut(s) 1170, 1376, 1780
HaeIII GGCC 7 cut(s) 669, 1017, 1282, 1325, 1689, 1832, 2497
HapII CCGG 1 cut(s) 288
Hin1I GRCGYC 1 cut(s) 69
HincII GTYRAC 1 cut(s) 2241
HindII GTYRAC 1 cut(s) 2241
HindIII AAGCTT 3 cut(s) 555, 2039, 2224
HpaI GTTAAC 1 cut(s) 2241
HpaII CCGG 1 cut(s) 288
HphI GGTGA 4 cut(s) 716, 1540, 2020, 2432
Hpy166II GTNNAC 4 cut(s) 836, 2241, 2350, 2381
Hpy8I GTNNAC 4 cut(s) 836, 2241, 2350, 2381
Hpy99I CGWCG 3 cut(s) 71, 137, 2583
HpyAV CCTTC 4 cut(s) 816, 873, 1322, 1945
HpyCH4III ACNGT 7 cut(s) 168, 199, 331, 412, 1672, 1934, 2008
HpyCH4IV ACGT 6 cut(s) 69, 135, 577, 1333, 2107, 2143
HpyF10VI GCNNNNNNNGC 8 cut(s) 105, 1160, 1169, 1184, 1732, 1994, 2117, 2165
HpySE526I ACGT 6 cut(s) 69, 135, 577, 1333, 2107, 2143
Hsp92I GRCGYC 1 cut(s) 69
Kpn2I TCCGGA 1 cut(s) 287
KspAI GTTAAC 1 cut(s) 2241
Kzo9I GATC 5 cut(s) 931, 1643, 1885, 1942, 2180
LguI GCTCTTC 1 cut(s) 2175
LmnI GCTCC 4 cut(s) 118, 497, 1151, 1445
Lsp1109I GCAGC 4 cut(s) 388, 1984, 2012, 2238
LweI GCATC 6 cut(s) 265, 838, 1550, 2270, 2506, 2551
MaeI CTAG 7 cut(s) 636, 687, 746, 959, 1118, 1421, 1536
MaeII ACGT 6 cut(s) 69, 135, 577, 1333, 2107, 2143
MaeIII GTNAC 4 cut(s) 296, 1603, 2065, 2272
MalI GATC 5 cut(s) 933, 1645, 1887, 1944, 2182
MbiI CCGCTC 1 cut(s) 500
MboI GATC 5 cut(s) 931, 1643, 1885, 1942, 2180
MfeI CAATTG 1 cut(s) 2316
MflI RGATCY 1 cut(s) 1643
MhlI GDGCHC 6 cut(s) 123, 1029, 1189, 1300, 1327, 1450
MlsI TGGCCA 3 cut(s) 1282, 1689, 1832
MluNI TGGCCA 3 cut(s) 1282, 1689, 1832
MlyI GAGTC 2 cut(s) 25, 1227
MmeI TCCRAC 2 cut(s) 90, 2493
Mox20I TGGCCA 3 cut(s) 1282, 1689, 1832
Mph1103I ATGCAT 1 cut(s) 473
MroI TCCGGA 1 cut(s) 287
MscI TGGCCA 3 cut(s) 1282, 1689, 1832
MseI TTAA 6 cut(s) 212, 249, 977, 2232, 2240, 2288
MslI CAYNNNNRTG 1 cut(s) 2266
Msp20I TGGCCA 3 cut(s) 1282, 1689, 1832
MspA1I CMGCKG 3 cut(s) 401, 783, 1988
MspI CCGG 1 cut(s) 288
MspR9I CCNGG 6 cut(s) 272, 439, 490, 1149, 1416, 1718
MunI CAATTG 1 cut(s) 2316
Mva1269I GAATGC 1 cut(s) 628
MvaI CCWGG 6 cut(s) 272, 439, 490, 1149, 1416, 1718
MvnI CGCG 1 cut(s) 448
MwoI GCNNNNNNNGC 8 cut(s) 105, 1160, 1169, 1184, 1732, 1994, 2117, 2165
NcoI CCATGG 3 cut(s) 927, 1283, 1892
NdeII GATC 5 cut(s) 931, 1643, 1885, 1942, 2180
NlaIV GGNNCC 7 cut(s) 58, 323, 668, 775, 1153, 1325, 1326
NmeAIII GCCGAG 1 cut(s) 134
NmuCI GTSAC 1 cut(s) 2272
NruI TCGCGA 1 cut(s) 448
NsiI ATGCAT 1 cut(s) 473
NspI RCATGY 3 cut(s) 471, 1412, 1502
NspV TTCGAA 1 cut(s) 28
PaeI GCATGC 1 cut(s) 1412
PaeR7I CTCGAG 1 cut(s) 2470
PagI TCATGA 1 cut(s) 763
PasI CCCWGGG 1 cut(s) 489
PciSI GCTCTTC 1 cut(s) 2175
PctI GAATGC 1 cut(s) 628
PfeI GAWTC 9 cut(s) 181, 284, 304, 1751, 2033, 2147, 2296, 2306, 2330
PfoI TCCNGGA 2 cut(s) 270, 1147
PkrI GCNGC 4 cut(s) 403, 1999, 2002, 2253
PleI GAGTC 2 cut(s) 24, 1227
PmaCI CACGTG 1 cut(s) 2108
PmlI CACGTG 1 cut(s) 2108
PpsI GAGTC 2 cut(s) 24, 1227
Ppu21I YACGTR 2 cut(s) 2108, 2144
PsiI TTATAA 1 cut(s) 2412
Psp124BI GAGCTC 1 cut(s) 1189
Psp6I CCWGG 6 cut(s) 270, 437, 488, 1147, 1414, 1716
PspCI CACGTG 1 cut(s) 2108
PspGI CCWGG 6 cut(s) 270, 437, 488, 1147, 1414, 1716
PspN4I GGNNCC 7 cut(s) 58, 323, 668, 775, 1153, 1325, 1326
PspOMI GGGCCC 1 cut(s) 1323
PspPI GGNCC 4 cut(s) 667, 773, 1323, 1324
PstI CTGCAG 2 cut(s) 1165, 2002
PsuI RGATCY 1 cut(s) 1643
PvuII CAGCTG 3 cut(s) 401, 783, 1988
RruI TCGCGA 1 cut(s) 448
RsaI GTAC 3 cut(s) 1182, 2142, 2219
RsaNI GTAC 3 cut(s) 1181, 2141, 2218
RseI CAYNNNNRTG 1 cut(s) 2266
SacI GAGCTC 1 cut(s) 1189
SapI GCTCTTC 1 cut(s) 2175
SaqAI TTAA 6 cut(s) 212, 249, 977, 2232, 2240, 2288
SatI GCNGC 4 cut(s) 402, 1998, 2001, 2252
Sau3AI GATC 5 cut(s) 931, 1643, 1885, 1942, 2180
Sau96I GGNCC 4 cut(s) 667, 773, 1323, 1324
SchI GAGTC 2 cut(s) 25, 1227
ScrFI CCNGG 6 cut(s) 272, 439, 490, 1149, 1416, 1718
SduI GDGCHC 6 cut(s) 123, 1029, 1189, 1300, 1327, 1450
SfaNI GCATC 6 cut(s) 265, 838, 1550, 2270, 2506, 2551
SfcI CTRYAG 2 cut(s) 1161, 1998
Sfr274I CTCGAG 1 cut(s) 2470
SfuI TTCGAA 1 cut(s) 28
SinI GGWCC 1 cut(s) 773
SlaI CTCGAG 1 cut(s) 2470
SmiMI CAYNNNNRTG 1 cut(s) 2266
SmlI CTYRAG 2 cut(s) 2459, 2470
SmoI CTYRAG 2 cut(s) 2459, 2470
SphI GCATGC 1 cut(s) 1412
SsiI CCGC 3 cut(s) 500, 866, 2337
SspMI CTAG 7 cut(s) 636, 687, 746, 959, 1118, 1421, 1536
SstI GAGCTC 1 cut(s) 1189
StyD4I CCNGG 6 cut(s) 270, 437, 488, 1147, 1414, 1716
StyI CCWWGG 4 cut(s) 927, 1283, 1440, 1892
TaaI ACNGT 7 cut(s) 168, 199, 331, 412, 1672, 1934, 2008
TaiI ACGT 6 cut(s) 72, 138, 580, 1336, 2110, 2146
TaqI TCGA 6 cut(s) 28, 988, 1225, 1635, 2471, 2581
TatI WGTACW 1 cut(s) 2217
TfiI GAWTC 9 cut(s) 181, 284, 304, 1751, 2033, 2147, 2296, 2306, 2330
Tru1I TTAA 6 cut(s) 212, 249, 977, 2232, 2240, 2288
Tru9I TTAA 6 cut(s) 212, 249, 977, 2232, 2240, 2288
TscAI CASTG 4 cut(s) 161, 204, 595, 1486
TseFI GTSAC 1 cut(s) 2272
TseI GCWGC 4 cut(s) 401, 1997, 2000, 2251
Tsp45I GTSAC 1 cut(s) 2272
TspDTI ATGAA 6 cut(s) 928, 993, 1264, 1580, 2205, 2288
TspGWI ACGGA 3 cut(s) 423, 1358, 2258
TspRI CASTG 4 cut(s) 161, 204, 595, 1486
VpaK11BI GGWCC 1 cut(s) 773
XagI CCTNNNNNAGG 1 cut(s) 1136
XapI RAATTY 6 cut(s) 214, 230, 1453, 1460, 1815, 2192
XbaI TCTAGA 1 cut(s) 1420
XceI RCATGY 3 cut(s) 471, 1412, 1502
XcmI CCANNNNNNNNNTGG 2 cut(s) 367, 1290
XhoI CTCGAG 1 cut(s) 2470
XmiI GTMKAC 1 cut(s) 2349
XspI CTAG 7 cut(s) 636, 687, 746, 959, 1118, 1421, 1536
ZraI GACGTC 1 cut(s) 70
Zsp2I ATGCAT 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.