pycom03g12610

Nuclear cap-binding protein subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0007238
Physical Location & Seq
Forward (+)
13273092 .. 13278366
5275 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g12610.4

Sequence Viewer

Length: 1386 bp
ATGTCGAACACGACTCATCCTTTGGTTTTCACACCACTTGTAACCGGAAACTTTCAGTTCATCTGGCCATGGGAAGAGTGGGCTCATGTCTTAGACCTTCCAAAATGGGCCCCACAACGTGTTTTTGTTCAGGAGGTTCTGGAGAGAGAAGTCCGTTTATCTTACTGGGATAAAGTGAAGCAGAGTATTGAGCATGCCCCTGGTCTGGAAGAGTTGCTTCCTCCCAAGGGAGCACCGAATTTCAAATTTAGTGTTCAAGAAACCAGCGAAGAAGATGGGCTACATGCACTTTCTTCTGACCTCAGAAACATGGTGAAAGCTAGGGCATCTGCCCGTGAAATGATAGTATGGATAGAGGAAAGTGTGTTTCCTGTTCATGGGATGGAAGTTACCCTCAATGTGGTTGTGCAAACTCTCCTCGATATTGGATCTAAAAGTTTCACTCATTTGATTACTGTATTGGAGAGATACGGCCAAGTCATTGCAAAACTATGTGCTGACCAGGATAAGCAGGTCATGCTGATAAATGAGATTGATTCTTACTGGAGGAATAATTCTCAAATGACTGCTATTGCAATAGATAGGATGATGGGCTACCGAATTTTGTCTAATCTGGCCATTGTCAGATGGGTCTTTTTACCAGCAAACATTGAGCAGTTTCACATATTAGATCGTCCATGGGAGATACTAAGAAATGCAGTTAGCAAGACATATAACCGTGTATGTGATCTACGGAAGGAGATTTCTTCTTTCAAAAAGAGTGTTGTATCGGCTGAAGAAGCTGCAGCAACGGTGAAAGCAGAGTTAGTAGCTTCTGAATCAAAGCTTTCACTCGTGGACGGCGAAGCTGTTACTGGGGAAAATCCTGTGTGGCTGAAGCGTTTGAAATCACGTGCTGAAAAGGCTGAGGAGGAGGTGGTGTCTGTACGTGAATCTTTAGAGGCTAAGCAGGCTCTTCTATCCCGATCCCTTGATGAATTTGAGGCATTATTCTACTCCATGTACAGAAGCTTCCTTAATGTGTTAACGGAACGGCTGCCAGAAGCATCCAAATGTGTCACATTGCAGGGATTAAAGGGGATTCATGCAGATTCTATGGCAGTTGACCTTGAAGAATCATCCGCAATGGATGTAGACAATGAGAATGGAATACCTAAGAAAAGTGAACTGAATGGTGGGAGGACAAGTAATGTTTATAATGTAGGGGAAAAAGAGCAATGGTGTCTTTCAACTTTAGGCTATCTCAAGGCGTTCTCGAGGCAATATGCTTCTGAGATTTGGCCTCACATGGAGAAGTTGGATGCTGAGGTTCTAACCGAAGACGTGCATCCTCTTATTCGGAAATCAGTTTATTGTGGTCTTCGTCGAGAAACTGATGGGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000184 GO:0000339 GO:0000375 GO:0000377 GO:0000394 GO:0000398 GO:0000956 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005845 GO:0005846 GO:0006139 GO:0006396 GO:0006397 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0008380 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009892 GO:0009987 GO:0010033 GO:0010228 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0019222 GO:0019439 GO:0022414 GO:0031047 GO:0031050 GO:0031053 GO:0031503 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033993 GO:0034470 GO:0034518 GO:0034613 GO:0034641 GO:0034655 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044464 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048519 GO:0048571 GO:0048573 GO:0048574 GO:0048608 GO:0048731 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070727 GO:0070887 GO:0070918 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0097159 GO:0097305 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901698 GO:1901699 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 map03013 map03015 map03040
Pfam Domains
Protein Families
MIF4G MIF4G_like MIF4G_like_2

Protein Analysis

462

Amino Acids

52.46

Weight (kDa)

5.37

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1197
Acc36I ACCTGC 1 cut(s) 502
AccI GTMKAC 1 cut(s) 1134
AciI CCGC 1 cut(s) 1122
AclWI GGATC 2 cut(s) 436, 960
AcoI YGGCCR 3 cut(s) 65, 472, 615
AcsI RAATTY 4 cut(s) 238, 245, 600, 977
AcuI CTGAAG 2 cut(s) 795, 896
AcvI CACGTG 1 cut(s) 893
AdeI CACNNNGTG 1 cut(s) 119
AfaI GTAC 2 cut(s) 927, 1004
AfiI CCNNNNNNNGG 4 cut(s) 205, 227, 377, 400
AflIII ACRYGT 1 cut(s) 118
AgsI TTSAA 6 cut(s) 244, 257, 754, 886, 1112, 1230
AjiI CACGTC 1 cut(s) 1324
AjnI CCWGG 2 cut(s) 199, 501
AluBI AGCT 6 cut(s) 320, 782, 812, 826, 848, 1011
AluI AGCT 6 cut(s) 320, 782, 812, 826, 848, 1011
Alw21I GWGCWC 1 cut(s) 235
AlwI GGATC 2 cut(s) 436, 960
Ama87I CYCGRG 1 cut(s) 1255
AoxI GGCC 5 cut(s) 65, 108, 472, 615, 1280
ApaI GGGCCC 1 cut(s) 112
ApeKI GCWGC 3 cut(s) 782, 785, 1036
ApoI RAATTY 4 cut(s) 238, 245, 600, 977
ArsI GACNNNNNNTTYG 1 cut(s) 36
AspS9I GGNCC 2 cut(s) 108, 109
AsuHPI GGTGA 2 cut(s) 325, 805
AvaI CYCGRG 1 cut(s) 1255
BaeGI GKGCMC 1 cut(s) 112
BalI TGGCCA 2 cut(s) 67, 617
BanII GRGCYC 2 cut(s) 85, 112
BarI GAAGNNNNNNTAC 4 cut(s) 264, 296, 995, 1027
BauI CACGAG 1 cut(s) 833
BbrPI CACGTG 1 cut(s) 893
BbsI GAAGAC 2 cut(s) 1326, 1352
Bbv12I GWGCWC 1 cut(s) 235
BbvCI CCTCAGC 2 cut(s) 906, 1305
BbvI GCAGC 3 cut(s) 769, 797, 1023
BccI CCATC 5 cut(s) 269, 376, 583, 621, 1370
BceAI ACGGC 3 cut(s) 487, 856, 1049
BciT130I CCWGG 2 cut(s) 201, 503
BfaI CTAG 1 cut(s) 321
BfmI CTRYAG 1 cut(s) 783
BfuAI ACCTGC 1 cut(s) 502
BisI GCNGC 3 cut(s) 783, 786, 1037
BlpI GCTNAGC 1 cut(s) 945
BlsI GCNGC 3 cut(s) 784, 787, 1038
Bme1390I CCNGG 2 cut(s) 201, 503
BmeT110I CYCGRG 1 cut(s) 1255
BmgBI CACGTC 1 cut(s) 1324
BmgT120I GGNCC 2 cut(s) 108, 109
BmiI GGNNCC 2 cut(s) 110, 111
BmrFI CCNGG 2 cut(s) 201, 503
BmrI ACTGGG 2 cut(s) 175, 864
BmsI GCATC 4 cut(s) 335, 1055, 1291, 1336
BmuI ACTGGG 2 cut(s) 175, 864
BpiI GAAGAC 2 cut(s) 1326, 1352
BpmI CTGGAG 2 cut(s) 161, 565
Bpu10I CCTNAGC 2 cut(s) 906, 1305
Bpu1102I GCTNAGC 1 cut(s) 945
BpuEI CTTGAG 1 cut(s) 1229
BsaAI YACGTR 2 cut(s) 893, 929
BsaJI CCNNGG 4 cut(s) 68, 199, 225, 677
BsaWI WCCGGW 1 cut(s) 44
BsaXI ACNNNNNCTCC 4 cut(s) 905, 935, 1171, 1201
Bsc4I CCNNNNNNNGG 4 cut(s) 205, 227, 377, 400
Bse1I ACTGG 3 cut(s) 170, 548, 859
Bse3DI GCAATG 4 cut(s) 480, 1061, 1131, 1223
BseBI CCWGG 2 cut(s) 201, 503
BseDI CCNNGG 4 cut(s) 68, 199, 225, 677
BseGI GGATG 8 cut(s) 16, 387, 591, 1046, 1118, 1135, 1306, 1327
BseLI CCNNNNNNNGG 4 cut(s) 205, 227, 377, 400
BseMI GCAATG 4 cut(s) 480, 1061, 1131, 1223
BseMII CTCAG 4 cut(s) 316, 897, 1263, 1296
BseNI ACTGG 3 cut(s) 170, 548, 859
BseRI GAGGAG 3 cut(s) 407, 923, 926
BseSI GKGCMC 1 cut(s) 112
BseXI GCAGC 3 cut(s) 769, 797, 1023
BshFI GGCC 5 cut(s) 67, 110, 474, 617, 1282
BsiHKAI GWGCWC 1 cut(s) 235
BsiHKCI CYCGRG 1 cut(s) 1255
BsiSI CCGG 1 cut(s) 45
BslI CCNNNNNNNGG 4 cut(s) 205, 227, 377, 400
BsnI GGCC 5 cut(s) 67, 110, 474, 617, 1282
BsoBI CYCGRG 1 cut(s) 1255
Bsp120I GGGCCC 1 cut(s) 108
Bsp1286I GDGCHC 3 cut(s) 85, 112, 235
Bsp1407I TGTACA 1 cut(s) 1002
Bsp143I GATC 4 cut(s) 428, 670, 727, 965
Bsp1720I GCTNAGC 1 cut(s) 945
Bsp19I CCATGG 2 cut(s) 68, 677
BspACI CCGC 1 cut(s) 1122
BspANI GGCC 5 cut(s) 67, 110, 474, 617, 1282
BspCNI CTCAG 4 cut(s) 315, 898, 1264, 1297
BspLI GGNNCC 2 cut(s) 110, 111
BspMAI CTGCAG 1 cut(s) 787
BspMI ACCTGC 1 cut(s) 502
BspPI GGATC 2 cut(s) 436, 960
BspQI GCTCTTC 1 cut(s) 960
BsrDI GCAATG 4 cut(s) 480, 1061, 1131, 1223
BsrGI TGTACA 1 cut(s) 1002
BsrI ACTGG 3 cut(s) 170, 548, 859
BssECI CCNNGG 4 cut(s) 68, 199, 225, 677
BssMI GATC 4 cut(s) 428, 670, 727, 965
BssSI CACGAG 1 cut(s) 833
BssT1I CCWWGG 3 cut(s) 68, 225, 677
Bst2BI CACGAG 1 cut(s) 833
Bst2UI CCWGG 2 cut(s) 201, 503
Bst4CI ACNGT 3 cut(s) 457, 719, 793
Bst6I CTCTTC 3 cut(s) 69, 204, 960
BstAPI GCANNNNNTGC 1 cut(s) 517
BstAUI TGTACA 1 cut(s) 1002
BstBAI YACGTR 2 cut(s) 893, 929
BstC8I GCNNGC 2 cut(s) 195, 951
BstDEI CTNAG 8 cut(s) 91, 302, 689, 906, 945, 1155, 1272, 1305
BstDSI CCRYGG 2 cut(s) 68, 677
BstF5I GGATG 8 cut(s) 16, 387, 591, 1046, 1118, 1135, 1306, 1327
BstKTI GATC 4 cut(s) 431, 673, 730, 968
BstMBI GATC 4 cut(s) 428, 670, 727, 965
BstMWI GCNNNNNNNGC 4 cut(s) 517, 779, 902, 950
BstNI CCWGG 2 cut(s) 201, 503
BstNSI RCATGY 2 cut(s) 197, 287
BstSCI CCNGG 2 cut(s) 199, 501
BstSFI CTRYAG 1 cut(s) 783
BstSLI GKGCMC 1 cut(s) 112
BstV1I GCAGC 3 cut(s) 769, 797, 1023
BstV2I GAAGAC 2 cut(s) 1326, 1352
BstX2I RGATCY 1 cut(s) 428
BstYI RGATCY 1 cut(s) 428
BsuRI GGCC 5 cut(s) 67, 110, 474, 617, 1282
BtgI CCRYGG 2 cut(s) 68, 677
BtrI CACGTC 1 cut(s) 1324
BtsCI GGATG 8 cut(s) 16, 387, 591, 1046, 1118, 1135, 1306, 1327
BveI ACCTGC 1 cut(s) 502
Cac8I GCNNGC 2 cut(s) 195, 951
Cfr13I GGNCC 2 cut(s) 108, 109
Csp6I GTAC 2 cut(s) 926, 1003
CviQI GTAC 2 cut(s) 926, 1003
DdeI CTNAG 8 cut(s) 91, 302, 689, 906, 945, 1155, 1272, 1305
DpnI GATC 4 cut(s) 430, 672, 729, 967
DpnII GATC 4 cut(s) 428, 670, 727, 965
DraIII CACNNNGTG 1 cut(s) 119
EaeI YGGCCR 3 cut(s) 65, 472, 615
Eam1104I CTCTTC 3 cut(s) 69, 204, 960
EarI CTCTTC 3 cut(s) 69, 204, 960
Eco130I CCWWGG 3 cut(s) 68, 225, 677
Eco24I GRGCYC 2 cut(s) 85, 112
Eco57I CTGAAG 2 cut(s) 795, 896
Eco72I CACGTG 1 cut(s) 893
Eco88I CYCGRG 1 cut(s) 1255
EcoO109I RGGNCCY 1 cut(s) 109
EcoRII CCWGG 2 cut(s) 199, 501
EcoT14I CCWWGG 3 cut(s) 68, 225, 677
EcoT38I GRGCYC 2 cut(s) 85, 112
ErhI CCWWGG 3 cut(s) 68, 225, 677
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FblI GTMKAC 1 cut(s) 1134
Fnu4HI GCNGC 3 cut(s) 783, 786, 1037
FokI GGATG 8 cut(s) 3, 394, 598, 1033, 1105, 1142, 1313, 1314
FriOI GRGCYC 2 cut(s) 85, 112
Fsp4HI GCNGC 3 cut(s) 783, 786, 1037
FspBI CTAG 1 cut(s) 321
GluI GCNGC 3 cut(s) 783, 786, 1037
GsuI CTGGAG 2 cut(s) 161, 565
HaeIII GGCC 5 cut(s) 67, 110, 474, 617, 1282
HapII CCGG 1 cut(s) 45
HincII GTYRAC 2 cut(s) 1026, 1105
HindII GTYRAC 2 cut(s) 1026, 1105
HindIII AAGCTT 2 cut(s) 824, 1009
HinfI GANTC 7 cut(s) 13, 536, 818, 932, 1081, 1091, 1115
HpaI GTTAAC 1 cut(s) 1026
HpaII CCGG 1 cut(s) 45
HphI GGTGA 2 cut(s) 325, 805
Hpy166II GTNNAC 5 cut(s) 838, 1026, 1105, 1135, 1166
Hpy188I TCNGA 6 cut(s) 298, 305, 626, 817, 1273, 1341
Hpy188III TCNNGA 7 cut(s) 131, 140, 206, 257, 963, 1255, 1367
Hpy8I GTNNAC 5 cut(s) 838, 1026, 1105, 1135, 1166
Hpy99I CGWCG 1 cut(s) 1368
HpyAV CCTTC 2 cut(s) 107, 730
HpyCH4III ACNGT 3 cut(s) 457, 719, 793
HpyCH4IV ACGT 4 cut(s) 118, 892, 928, 1323
HpyCH4V TGCA 9 cut(s) 287, 409, 485, 575, 698, 785, 1066, 1088, 1327
HpyF10VI GCNNNNNNNGC 4 cut(s) 517, 779, 902, 950
HpyF3I CTNAG 8 cut(s) 91, 302, 689, 906, 945, 1155, 1272, 1305
HpySE526I ACGT 4 cut(s) 118, 892, 928, 1323
KspAI GTTAAC 1 cut(s) 1026
Kzo9I GATC 4 cut(s) 428, 670, 727, 965
LguI GCTCTTC 1 cut(s) 960
LmnI GCTCC 1 cut(s) 230
Lsp1109I GCAGC 3 cut(s) 769, 797, 1023
LweI GCATC 4 cut(s) 335, 1055, 1291, 1336
MaeI CTAG 1 cut(s) 321
MaeII ACGT 4 cut(s) 118, 892, 928, 1323
MaeIII GTNAC 4 cut(s) 40, 388, 850, 1057
MalI GATC 4 cut(s) 430, 672, 729, 967
MboI GATC 4 cut(s) 428, 670, 727, 965
MflI RGATCY 1 cut(s) 428
MhlI GDGCHC 3 cut(s) 85, 112, 235
MlsI TGGCCA 2 cut(s) 67, 617
MluCI AATT 5 cut(s) 238, 245, 553, 600, 977
MluNI TGGCCA 2 cut(s) 67, 617
MlyI GAGTC 1 cut(s) 7
MmeI TCCRAC 1 cut(s) 1278
Mox20I TGGCCA 2 cut(s) 67, 617
MscI TGGCCA 2 cut(s) 67, 617
MseI TTAA 3 cut(s) 1017, 1025, 1073
MslI CAYNNNNRTG 1 cut(s) 1051
Msp20I TGGCCA 2 cut(s) 67, 617
MspI CCGG 1 cut(s) 45
MspR9I CCNGG 2 cut(s) 201, 503
MvaI CCWGG 2 cut(s) 201, 503
MwoI GCNNNNNNNGC 4 cut(s) 517, 779, 902, 950
NcoI CCATGG 2 cut(s) 68, 677
NdeII GATC 4 cut(s) 428, 670, 727, 965
NlaIV GGNNCC 2 cut(s) 110, 111
NmuCI GTSAC 1 cut(s) 1057
NspI RCATGY 2 cut(s) 197, 287
PaeI GCATGC 1 cut(s) 197
PaeR7I CTCGAG 1 cut(s) 1255
PciSI GCTCTTC 1 cut(s) 960
PcsI WCGNNNNNNNCGW 1 cut(s) 840
PfeI GAWTC 6 cut(s) 536, 818, 932, 1081, 1091, 1115
PkrI GCNGC 3 cut(s) 784, 787, 1038
PleI GAGTC 1 cut(s) 7
PmaCI CACGTG 1 cut(s) 893
PmlI CACGTG 1 cut(s) 893
PpsI GAGTC 1 cut(s) 7
Ppu21I YACGTR 2 cut(s) 893, 929
PsiI TTATAA 1 cut(s) 1197
Psp6I CCWGG 2 cut(s) 199, 501
PspCI CACGTG 1 cut(s) 893
PspGI CCWGG 2 cut(s) 199, 501
PspN4I GGNNCC 2 cut(s) 110, 111
PspOMI GGGCCC 1 cut(s) 108
PspPI GGNCC 2 cut(s) 108, 109
PstI CTGCAG 1 cut(s) 787
PsuI RGATCY 1 cut(s) 428
RsaI GTAC 2 cut(s) 927, 1004
RsaNI GTAC 2 cut(s) 926, 1003
RseI CAYNNNNRTG 1 cut(s) 1051
SapI GCTCTTC 1 cut(s) 960
SaqAI TTAA 3 cut(s) 1017, 1025, 1073
SatI GCNGC 3 cut(s) 783, 786, 1037
Sau3AI GATC 4 cut(s) 428, 670, 727, 965
Sau96I GGNCC 2 cut(s) 108, 109
SchI GAGTC 1 cut(s) 7
ScrFI CCNGG 2 cut(s) 201, 503
SduI GDGCHC 3 cut(s) 85, 112, 235
SfaNI GCATC 4 cut(s) 335, 1055, 1291, 1336
SfcI CTRYAG 1 cut(s) 783
Sfr274I CTCGAG 1 cut(s) 1255
SlaI CTCGAG 1 cut(s) 1255
SmiMI CAYNNNNRTG 1 cut(s) 1051
SmlI CTYRAG 2 cut(s) 1244, 1255
SmoI CTYRAG 2 cut(s) 1244, 1255
SphI GCATGC 1 cut(s) 197
Sse9I AATT 5 cut(s) 238, 245, 553, 600, 977
SsiI CCGC 1 cut(s) 1122
SspMI CTAG 1 cut(s) 321
StyD4I CCNGG 2 cut(s) 199, 501
StyI CCWWGG 3 cut(s) 68, 225, 677
TaaI ACNGT 3 cut(s) 457, 719, 793
TaiI ACGT 4 cut(s) 121, 895, 931, 1326
TaqI TCGA 4 cut(s) 5, 420, 1256, 1366
TasI AATT 5 cut(s) 238, 245, 553, 600, 977
TatI WGTACW 1 cut(s) 1002
TfiI GAWTC 6 cut(s) 536, 818, 932, 1081, 1091, 1115
Tru1I TTAA 3 cut(s) 1017, 1025, 1073
Tru9I TTAA 3 cut(s) 1017, 1025, 1073
TseFI GTSAC 1 cut(s) 1057
TseI GCWGC 3 cut(s) 782, 785, 1036
Tsp45I GTSAC 1 cut(s) 1057
TspDTI ATGAA 4 cut(s) 49, 365, 990, 1073
TspGWI ACGGA 3 cut(s) 143, 748, 1043
XapI RAATTY 4 cut(s) 238, 245, 600, 977
XceI RCATGY 2 cut(s) 197, 287
XcmI CCANNNNNNNNNTGG 1 cut(s) 75
XhoI CTCGAG 1 cut(s) 1255
XmiI GTMKAC 1 cut(s) 1134
XspI CTAG 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.