Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0008187

Physical Location & Seq

Genomic Coordinates Reverse (-)

23797797 .. 23802654

Gene Structure 4858 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1173900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1752 bp

ATGGGAAATTGCTGTGTAACCCTTGGCGCACCTGGCCAGAACAAGAACAAGAACAACAAGAAGAAGACAAACCCATTTGCAACAGATTTTGGTGGCATTAATGGAAAAGATGGTGATGGCAAGCTGTTGGTCTTGAAAGACCCCACAGGTCGAGACATTTCGGCCCAATATGATCTGGGACGCGAACTTGGCCGAGGTGAATTCGGGGTAACATATCTGTGCACAGAGGCATCCTCCGACGAAAAATTTGCATGCAAATCGATATCAAAGAAGAAGCTTAGAACAGCGGTGGATATTGAGGATGTGAGGAGGGAAGTTGAGATAATGCAGCATTTGCCTCCACACCCAAATATTGTGACATTAAAGGACACTTATGAAGATGGTGATGCGGTTCACATTGTGATGGAGTTGTGTGAGGGCGGTGAGCTGTTCGACCGCATTGTTGCTAGGGGTCATTACACAGAACGTGCTGCTGCAGGCGTAATGAGGACGATTGTTGAAGTAGTTCAGATGTGTCATAAACACGGAGTAATGCATCGTGATCTCAAACCAGAAAACTTCCTATTTGCAAATAAGAAGGAAACAGCCTCTTTGAAAGCAATTGATTTTGGACTGTCTGTGTTCTTTAAACCTGGTGAGCAATTTAATGAGATAGTGGGCAGTCCTTATTACATGGCTCCAGAGGTTCTGAAGCGCAACTATGGACCCGAGGTTGACATTTGGAGTGCTGGGGTTATTCTATATATTTTACTTTGTGGTGTTCCACCTTTCTGGGCAGAAACTGAACAAGGGGTAGCACAAGCAATTATTCGATCCGTCGTTGATTTCAAGAGGGACCCCTGGCCTAGAGTTTCTGATAATGCAAAGGACCTTGTGAAGAAAATGCTTGATCCTGATCCGAAGAAGAGGCTGACAGCTAAGGAAGTGCTTGATCATCCTTGGATACAAAATGCCAAGAAGGCCCCAAATGTCCCACTTGGTGAAACTGTGAGAGCAAGGCTGCAACAGTTTTCTGTAATGAACAAGCTGAAGAAAAGAGCTCTTGGGGTCATAGCTGAGCATTTATCAGTTGCGGAAGTAGCTGGCATAAAAGAGGCTTTTCAAATGATGGACACTGGCAACAAGGGCAAGGTGACCATAGAAGAGCTACGAAGCGGGATACAACAGTTAGGGCAAAATATTTCTGATCCAGATCTTCAAATACTAATGGAAGCTGCTGATGTTGATGGGGATGGAGCTCTAAACTATGGAGAGTTTGTTGTTGTCACGGTTCACCTTAAAAAGATGGCGAATGACGAGCACCTACATAAAGCCTTTGCCTTCTTTGATCAAAACCAAAGTGGCCATATAGAAATTGAAGAATTGAGAAATGCGTTAAATAATGATGTTGACGTGGGTGGTGAGGAAGTCATCCATGCGATTATGCATGACGTGGACACAGATAAGGATGGACGCATAAGTTATGAGGAGTTTGCTGCTATGATGAAGGCTGGTACAGATTGGAGAAAAGCATCTAGACAATATTCACGGGAAAGATTCAACAGCATAAGCCTGAAGTTGATGAGAGAAGGGTCTTTTACAGTTGGCAATGAGGGTAGCGTGATGACCAAAACTCAGCTGCCTATGTGTTTTGAATGGGTATCAAATTCCTTGCCAAGGTGCAAACGATTCGTGCCTGCTAGTTCTTCTGCGGACTCTCGTTGTCCATCAGATCATCATGGACCATCACGGTTGACGGCTTGCCTACCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8 Pkinase

Protein Analysis

584

Amino Acids

64.93

Weight (kDa)

6.31

Isoelectric Point (pI)

39.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	57 - 315	4.1e-81	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	59 - 310	2.3e-26	Protein tyrosine and serine/threonine kinase
EF-hand_7	PF13499	363 - 420	3e-11	EF-hand domain pair
EF-hand_8	PF13833	375 - 425	2.9e-06	EF-hand domain pair
EF-hand_7	PF13499	433 - 496	1.1e-15	EF-hand domain pair
EF-hand_1	PF00036	471 - 497	7.1e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008187)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12480 AT5G12480 AT5G19450 AT5G19450
fragaria_vesca	FvH4_3g28740 FvH4_3g28740 FvH4_3g28740 FvH4_3g28740
malus_domestica	MD03G1173900.v1.1 MD11G1192100.v1.1
prunus_persica	Prupe.4G250200_v2.0.a1 Prupe.4G250200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0052321
rosa_laevigata	RLG00000034825
rosa_multiflora	Rmu_sc0000968.1_g000007 Rmu_sc0028377.1_g000006
rosa_roxburghii	Rroxscaffold_1G00028230
rosa_rugosa	Rorug05G0272800 Rorug05G0272900
rosa_samantha	Rh5AG345400 Rh5CG381800 Rh5DG371600
rosa_wichuraiana	Rw5G032600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	183
AciI	CCGC	7 cut(s)	287, 389, 420, 436, 1073, 1155, 1691
AclWI	GGATC	4 cut(s)	807, 884, 890, 1181
AcoI	YGGCCR	3 cut(s)	34, 190, 1342
AcsI	RAATTY	3 cut(s)	200, 245, 1645
AcuI	CTGAAG	3 cut(s)	710, 1049, 1574
AdeI	CACNNNGTG	2 cut(s)	400, 980
AfaI	GTAC	1 cut(s)	1495
AfiI	CCNNNNNNNGG	1 cut(s)	1656
AgsI	TTSAA	9 cut(s)	136, 500, 595, 829, 1103, 1199, 1358, 1540, 1634
AjiI	CACGTC	2 cut(s)	1393, 1432
AjnI	CCWGG	3 cut(s)	31, 631, 839
Alw21I	GWGCWC	4 cut(s)	224, 1042, 1240, 1302
Alw26I	GTCTC	1 cut(s)	147
Alw44I	GTGCAC	1 cut(s)	220
AlwI	GGATC	4 cut(s)	807, 884, 890, 1181
AlwNI	CAGNNNCTG	1 cut(s)	782
Ama87I	CYCGRG	1 cut(s)	707
AoxI	GGCC	6 cut(s)	34, 162, 190, 842, 960, 1342
ApaLI	GTGCAC	1 cut(s)	220
ApeKI	GCWGC	7 cut(s)	328, 470, 473, 1000, 1214, 1475, 1618
ApoI	RAATTY	3 cut(s)	200, 245, 1645
ArsI	GACNNNNNNTTYG	2 cut(s)	230, 262
AseI	ATTAAT	1 cut(s)	99
Asp700I	GAANNNNTTC	1 cut(s)	504
AspLEI	GCGC	2 cut(s)	29, 696
AspS9I	GGNCC	6 cut(s)	163, 704, 835, 868, 961, 1721
AsuHPI	GGTGA	9 cut(s)	125, 209, 395, 434, 647, 992, 1144, 1265, 1412
AvaI	CYCGRG	1 cut(s)	707
AvaII	GGWCC	4 cut(s)	704, 835, 868, 1721
BaeGI	GKGCMC	1 cut(s)	224
BaeI	ACNNNNGTAYC	2 cut(s)	1151, 1184
BalI	TGGCCA	2 cut(s)	36, 1344
BanII	GRGCYC	2 cut(s)	1042, 1240
BbsI	GAAGAC	1 cut(s)	71
Bbv12I	GWGCWC	4 cut(s)	224, 1042, 1240, 1302
BbvI	GCAGC	7 cut(s)	340, 457, 460, 987, 1201, 1462, 1605
BcgI	CGANNNNNNTGC	6 cut(s)	230, 240, 264, 274, 1651, 1685
BciT130I	CCWGG	3 cut(s)	33, 633, 841
BciVI	GTATCC	2 cut(s)	936, 1152
BclI	TGATCA	2 cut(s)	931, 1327
BcoDI	GTCTC	1 cut(s)	147
BfaI	CTAG	4 cut(s)	447, 846, 1515, 1680
BfmI	CTRYAG	1 cut(s)	474
BfuI	GTATCC	2 cut(s)	936, 1152
BglI	GCCNNNNNGGC	1 cut(s)	959
BglII	AGATCT	1 cut(s)	1192
BisI	GCNGC	7 cut(s)	329, 471, 474, 1001, 1215, 1476, 1619
BlpI	GCTNAGC	1 cut(s)	1056
BlsI	GCNGC	7 cut(s)	330, 472, 475, 1002, 1216, 1477, 1620
Bme1390I	CCNGG	3 cut(s)	33, 633, 841
Bme18I	GGWCC	4 cut(s)	704, 835, 868, 1721
BmeT110I	CYCGRG	1 cut(s)	707
BmgBI	CACGTC	2 cut(s)	1393, 1432
BmgT120I	GGNCC	6 cut(s)	163, 704, 835, 868, 961, 1721
BmiI	GGNNCC	5 cut(s)	678, 706, 836, 837, 963
BmrFI	CCNGG	3 cut(s)	33, 633, 841
BmsI	GCATC	4 cut(s)	239, 376, 544, 1520
BpiI	GAAGAC	1 cut(s)	71
BplI	GAGNNNNNCTC	2 cut(s)	218, 250
BpmI	CTGGAG	1 cut(s)	663
Bpu10I	CCTNAGC	1 cut(s)	918
Bpu1102I	GCTNAGC	1 cut(s)	1056
Bsa29I	ATCGAT	1 cut(s)	260
BsaBI	GATNNNNATC	2 cut(s)	894, 1191
BsaJI	CCNNGG	6 cut(s)	22, 193, 708, 839, 938, 1655
BsaXI	ACNNNNNCTCC	2 cut(s)	1242, 1272
Bsc4I	CCNNNNNNNGG	1 cut(s)	1656
Bse1I	ACTGG	1 cut(s)	1120
Bse3DI	GCAATG	1 cut(s)	1594
Bse8I	GATNNNNATC	2 cut(s)	894, 1191
BseBI	CCWGG	3 cut(s)	33, 633, 841
BseCI	ATCGAT	1 cut(s)	260
BseDI	CCNNGG	6 cut(s)	22, 193, 708, 839, 938, 1655
BseGI	GGATG	6 cut(s)	230, 307, 934, 1237, 1410, 1453
BseJI	GATNNNNATC	2 cut(s)	894, 1191
BseLI	CCNNNNNNNGG	1 cut(s)	1656
BseMI	GCAATG	1 cut(s)	1594
BseMII	CTCAG	2 cut(s)	1047, 1628
BseNI	ACTGG	1 cut(s)	1120
BseRI	GAGGAG	2 cut(s)	322, 1481
BseSI	GKGCMC	1 cut(s)	224
BseXI	GCAGC	7 cut(s)	340, 457, 460, 987, 1201, 1462, 1605
BseYI	CCCAGC	1 cut(s)	728
Bsh1236I	CGCG	1 cut(s)	183
Bsh1285I	CGRYCG	1 cut(s)	436
BshFI	GGCC	6 cut(s)	36, 164, 192, 844, 962, 1344
BshVI	ATCGAT	1 cut(s)	260
BsiEI	CGRYCG	1 cut(s)	436
BsiHKAI	GWGCWC	4 cut(s)	224, 1042, 1240, 1302
BsiHKCI	CYCGRG	1 cut(s)	707
BslFI	GGGAC	3 cut(s)	192, 848, 956
BslI	CCNNNNNNNGG	1 cut(s)	1656
BsmAI	GTCTC	1 cut(s)	147
BsmFI	GGGAC	3 cut(s)	192, 848, 956
BsnI	GGCC	6 cut(s)	36, 164, 192, 844, 962, 1344
BsoBI	CYCGRG	1 cut(s)	707
Bsp1286I	GDGCHC	4 cut(s)	224, 1042, 1240, 1302
Bsp1720I	GCTNAGC	1 cut(s)	1056
BspACI	CCGC	7 cut(s)	287, 389, 420, 436, 1073, 1155, 1691
BspANI	GGCC	6 cut(s)	36, 164, 192, 844, 962, 1344
BspCNI	CTCAG	2 cut(s)	1048, 1627
BspDI	ATCGAT	1 cut(s)	260
BspFNI	CGCG	1 cut(s)	183
BspLI	GGNNCC	5 cut(s)	678, 706, 836, 837, 963
BspMAI	CTGCAG	1 cut(s)	478
BspPI	GGATC	4 cut(s)	807, 884, 890, 1181
BspQI	GCTCTTC	1 cut(s)	1137
BsrDI	GCAATG	1 cut(s)	1594
BsrI	ACTGG	1 cut(s)	1120
BssECI	CCNNGG	6 cut(s)	22, 193, 708, 839, 938, 1655
BssT1I	CCWWGG	3 cut(s)	22, 938, 1655
Bst2UI	CCWGG	3 cut(s)	33, 633, 841
Bst4CI	ACNGT	8 cut(s)	615, 988, 1008, 1167, 1270, 1582, 1731, 1749
Bst6I	CTCTTC	2 cut(s)	899, 1137
BstAPI	GCANNNNNTGC	1 cut(s)	334
BstC8I	GCNNGC	6 cut(s)	122, 253, 478, 1084, 1677, 1741
BstDEI	CTNAG	4 cut(s)	278, 918, 1056, 1614
BstEII	GGTNACC	1 cut(s)	1132
BstENI	CCTNNNNNAGG	1 cut(s)	1654
BstF5I	GGATG	6 cut(s)	230, 307, 934, 1237, 1410, 1453
BstFNI	CGCG	1 cut(s)	183
BstHHI	GCGC	2 cut(s)	29, 696
BstMAI	GTCTC	1 cut(s)	147
BstMCI	CGRYCG	1 cut(s)	436
BstMWI	GCNNNNNNNGC	6 cut(s)	33, 189, 334, 959, 1079, 1125
BstNI	CCWGG	3 cut(s)	33, 633, 841
BstNSI	RCATGY	1 cut(s)	255
BstPI	GGTNACC	1 cut(s)	1132
BstSCI	CCNGG	3 cut(s)	31, 631, 839
BstSFI	CTRYAG	1 cut(s)	474
BstSLI	GKGCMC	1 cut(s)	224
BstUI	CGCG	1 cut(s)	183
BstV1I	GCAGC	7 cut(s)	340, 457, 460, 987, 1201, 1462, 1605
BstV2I	GAAGAC	1 cut(s)	71
BstX2I	RGATCY	1 cut(s)	1192
BstXI	CCANNNNNNTGG	1 cut(s)	771
BstYI	RGATCY	1 cut(s)	1192
Bsu15I	ATCGAT	1 cut(s)	260
BsuI	GTATCC	2 cut(s)	936, 1152
BsuRI	GGCC	6 cut(s)	36, 164, 192, 844, 962, 1344
BsuTUI	ATCGAT	1 cut(s)	260
BtrI	CACGTC	2 cut(s)	1393, 1432
BtsCI	GGATG	6 cut(s)	230, 307, 934, 1237, 1410, 1453
BtsIMutI	CAGTG	1 cut(s)	1113
Cac8I	GCNNGC	6 cut(s)	122, 253, 478, 1084, 1677, 1741
CaiI	CAGNNNCTG	1 cut(s)	782
CfoI	GCGC	2 cut(s)	29, 696
Cfr13I	GGNCC	6 cut(s)	163, 704, 835, 868, 961, 1721
ClaI	ATCGAT	1 cut(s)	260
CseI	GACGC	2 cut(s)	189, 1461
CsiI	ACCWGGT	1 cut(s)	631
Csp6I	GTAC	1 cut(s)	1494
CviAII	CATG	5 cut(s)	252, 673, 1415, 1427, 1718
CviQI	GTAC	1 cut(s)	1494
DdeI	CTNAG	4 cut(s)	278, 918, 1056, 1614
DraI	TTTAAA	1 cut(s)	628
DraIII	CACNNNGTG	2 cut(s)	400, 980
EaeI	YGGCCR	3 cut(s)	34, 190, 1342
Eam1104I	CTCTTC	2 cut(s)	899, 1137
EarI	CTCTTC	2 cut(s)	899, 1137
Ecl136II	GAGCTC	2 cut(s)	1040, 1238
Eco130I	CCWWGG	3 cut(s)	22, 938, 1655
Eco24I	GRGCYC	2 cut(s)	1042, 1240
Eco32I	GATATC	1 cut(s)	264
Eco47I	GGWCC	4 cut(s)	704, 835, 868, 1721
Eco53kI	GAGCTC	2 cut(s)	1040, 1238
Eco57I	CTGAAG	3 cut(s)	710, 1049, 1574
Eco88I	CYCGRG	1 cut(s)	707
Eco91I	GGTNACC	1 cut(s)	1132
EcoICRI	GAGCTC	2 cut(s)	1040, 1238
EcoNI	CCTNNNNNAGG	1 cut(s)	1654
EcoO109I	RGGNCCY	3 cut(s)	835, 868, 961
EcoO65I	GGTNACC	1 cut(s)	1132
EcoRI	GAATTC	1 cut(s)	200
EcoRII	CCWGG	3 cut(s)	31, 631, 839
EcoRV	GATATC	1 cut(s)	264
EcoT14I	CCWWGG	3 cut(s)	22, 938, 1655
EcoT22I	ATGCAT	2 cut(s)	537, 1428
EcoT38I	GRGCYC	2 cut(s)	1042, 1240
ErhI	CCWWGG	3 cut(s)	22, 938, 1655
FaeI	CATG	5 cut(s)	255, 676, 1418, 1430, 1721
FaqI	GGGAC	3 cut(s)	192, 848, 956
FatI	CATG	5 cut(s)	251, 672, 1414, 1426, 1717
FauI	CCCGC	1 cut(s)	1148
FbaI	TGATCA	2 cut(s)	931, 1327
Fnu4HI	GCNGC	7 cut(s)	329, 471, 474, 1001, 1215, 1476, 1619
FokI	GGATG	6 cut(s)	217, 314, 921, 1244, 1397, 1460
FriOI	GRGCYC	2 cut(s)	1042, 1240
Fsp4HI	GCNGC	7 cut(s)	329, 471, 474, 1001, 1215, 1476, 1619
FspBI	CTAG	4 cut(s)	447, 846, 1515, 1680
GlaI	GCGC	2 cut(s)	28, 695
GluI	GCNGC	7 cut(s)	329, 471, 474, 1001, 1215, 1476, 1619
GsaI	CCCAGC	1 cut(s)	732
GsuI	CTGGAG	1 cut(s)	663
HaeIII	GGCC	6 cut(s)	36, 164, 192, 844, 962, 1344
HgaI	GACGC	2 cut(s)	189, 1461
HhaI	GCGC	2 cut(s)	29, 696
Hin1II	CATG	5 cut(s)	255, 676, 1418, 1430, 1721
Hin6I	GCGC	2 cut(s)	27, 694
HinP1I	GCGC	2 cut(s)	27, 694
HincII	GTYRAC	3 cut(s)	715, 1390, 1734
HindII	GTYRAC	3 cut(s)	715, 1390, 1734
HindIII	AAGCTT	1 cut(s)	275
HinfI	GANTC	3 cut(s)	1536, 1668, 1694
HphI	GGTGA	9 cut(s)	125, 209, 395, 434, 647, 992, 1144, 1265, 1412
Hpy166II	GTNNAC	7 cut(s)	222, 394, 715, 1273, 1390, 1435, 1734
Hpy188I	TCNGA	7 cut(s)	238, 510, 690, 856, 900, 1186, 1711
Hpy188III	TCNNGA	8 cut(s)	133, 152, 539, 680, 829, 893, 1190, 1515
Hpy8I	GTNNAC	7 cut(s)	222, 394, 715, 1273, 1390, 1435, 1734
Hpy99I	CGWCG	2 cut(s)	242, 821
HpyAV	CCTTC	5 cut(s)	571, 952, 1330, 1480, 1562
HpyCH4III	ACNGT	8 cut(s)	615, 988, 1008, 1167, 1270, 1582, 1731, 1749
HpyCH4IV	ACGT	3 cut(s)	466, 1392, 1431
HpyF10VI	GCNNNNNNNGC	6 cut(s)	33, 189, 334, 959, 1079, 1125
HpyF3I	CTNAG	4 cut(s)	278, 918, 1056, 1614
HpySE526I	ACGT	3 cut(s)	466, 1392, 1431
Hsp92II	CATG	5 cut(s)	255, 676, 1418, 1430, 1721
HspAI	GCGC	2 cut(s)	27, 694
KflI	GGGWCCC	1 cut(s)	835
Ksp22I	TGATCA	2 cut(s)	931, 1327
LguI	GCTCTTC	1 cut(s)	1137
LmnI	GCTCC	2 cut(s)	682, 1235
Lsp1109I	GCAGC	7 cut(s)	340, 457, 460, 987, 1201, 1462, 1605
LweI	GCATC	4 cut(s)	239, 376, 544, 1520
MabI	ACCWGGT	1 cut(s)	631
MaeI	CTAG	4 cut(s)	447, 846, 1515, 1680
MaeII	ACGT	3 cut(s)	466, 1392, 1431
MaeIII	GTNAC	5 cut(s)	16, 208, 355, 1132, 1264
MfeI	CAATTG	1 cut(s)	600
MflI	RGATCY	1 cut(s)	1192
MhlI	GDGCHC	4 cut(s)	224, 1042, 1240, 1302
MlsI	TGGCCA	2 cut(s)	36, 1344
MluCI	AATT	9 cut(s)	7, 200, 245, 600, 641, 804, 1353, 1361, 1645
MluNI	TGGCCA	2 cut(s)	36, 1344
MlyI	GAGTC	1 cut(s)	1688
MmeI	TCCRAC	1 cut(s)	261
Mox20I	TGGCCA	2 cut(s)	36, 1344
Mph1103I	ATGCAT	2 cut(s)	537, 1428
MroXI	GAANNNNTTC	1 cut(s)	504
MscI	TGGCCA	2 cut(s)	36, 1344
MseI	TTAA	6 cut(s)	99, 362, 627, 645, 1278, 1376
MslI	CAYNNNNRTG	2 cut(s)	217, 401
Msp20I	TGGCCA	2 cut(s)	36, 1344
MspA1I	CMGCKG	2 cut(s)	287, 1618
MspR9I	CCNGG	3 cut(s)	33, 633, 841
MunI	CAATTG	1 cut(s)	600
MvaI	CCWGG	3 cut(s)	33, 633, 841
MvnI	CGCG	1 cut(s)	183
MwoI	GCNNNNNNNGC	6 cut(s)	33, 189, 334, 959, 1079, 1125
NlaIII	CATG	5 cut(s)	255, 676, 1418, 1430, 1721
NlaIV	GGNNCC	5 cut(s)	678, 706, 836, 837, 963
NmeAIII	GCCGAG	1 cut(s)	218
NmuCI	GTSAC	3 cut(s)	355, 1132, 1264
NsiI	ATGCAT	2 cut(s)	537, 1428
NspI	RCATGY	1 cut(s)	255
PaeI	GCATGC	1 cut(s)	255
PciSI	GCTCTTC	1 cut(s)	1137
PdmI	GAANNNNTTC	1 cut(s)	504
PfeI	GAWTC	2 cut(s)	1536, 1668
PkrI	GCNGC	7 cut(s)	330, 472, 475, 1002, 1216, 1477, 1620
PleI	GAGTC	1 cut(s)	1688
PpsI	GAGTC	1 cut(s)	1688
PpuMI	RGGWCCY	2 cut(s)	835, 868
PshBI	ATTAAT	1 cut(s)	99
Psp124BI	GAGCTC	2 cut(s)	1042, 1240
Psp5II	RGGWCCY	2 cut(s)	835, 868
Psp6I	CCWGG	3 cut(s)	31, 631, 839
PspEI	GGTNACC	1 cut(s)	1132
PspFI	CCCAGC	1 cut(s)	728
PspGI	CCWGG	3 cut(s)	31, 631, 839
PspN4I	GGNNCC	5 cut(s)	678, 706, 836, 837, 963
PspPI	GGNCC	6 cut(s)	163, 704, 835, 868, 961, 1721
PspPPI	RGGWCCY	2 cut(s)	835, 868
PstI	CTGCAG	1 cut(s)	478
PstNI	CAGNNNCTG	1 cut(s)	782
PsuI	RGATCY	1 cut(s)	1192
PvuII	CAGCTG	1 cut(s)	1618
RsaI	GTAC	1 cut(s)	1495
RsaNI	GTAC	1 cut(s)	1494
RseI	CAYNNNNRTG	2 cut(s)	217, 401
SacI	GAGCTC	2 cut(s)	1042, 1240
SapI	GCTCTTC	1 cut(s)	1137
SaqAI	TTAA	6 cut(s)	99, 362, 627, 645, 1278, 1376
SatI	GCNGC	7 cut(s)	329, 471, 474, 1001, 1215, 1476, 1619
Sau96I	GGNCC	6 cut(s)	163, 704, 835, 868, 961, 1721
SchI	GAGTC	1 cut(s)	1688
ScrFI	CCNGG	3 cut(s)	33, 633, 841
SduI	GDGCHC	4 cut(s)	224, 1042, 1240, 1302
SexAI	ACCWGGT	1 cut(s)	631
SfaNI	GCATC	4 cut(s)	239, 376, 544, 1520
SfcI	CTRYAG	1 cut(s)	474
SinI	GGWCC	4 cut(s)	704, 835, 868, 1721
SmiMI	CAYNNNNRTG	2 cut(s)	217, 401
SphI	GCATGC	1 cut(s)	255
Sse9I	AATT	9 cut(s)	7, 200, 245, 600, 641, 804, 1353, 1361, 1645
SsiI	CCGC	7 cut(s)	287, 389, 420, 436, 1073, 1155, 1691
SspI	AATATT	3 cut(s)	352, 1180, 1523
SspMI	CTAG	4 cut(s)	447, 846, 1515, 1680
SstI	GAGCTC	2 cut(s)	1042, 1240
StyD4I	CCNGG	3 cut(s)	31, 631, 839
StyI	CCWWGG	3 cut(s)	22, 938, 1655
TaaI	ACNGT	8 cut(s)	615, 988, 1008, 1167, 1270, 1582, 1731, 1749
TaiI	ACGT	3 cut(s)	469, 1395, 1434
TaqI	TCGA	4 cut(s)	151, 260, 432, 811
TasI	AATT	9 cut(s)	7, 200, 245, 600, 641, 804, 1353, 1361, 1645
TfiI	GAWTC	2 cut(s)	1536, 1668
Tru1I	TTAA	6 cut(s)	99, 362, 627, 645, 1278, 1376
Tru9I	TTAA	6 cut(s)	99, 362, 627, 645, 1278, 1376
TscAI	CASTG	1 cut(s)	1120
TseFI	GTSAC	3 cut(s)	355, 1132, 1264
TseI	GCWGC	7 cut(s)	328, 470, 473, 1000, 1214, 1475, 1618
Tsp45I	GTSAC	3 cut(s)	355, 1132, 1264
TspDTI	ATGAA	3 cut(s)	390, 1034, 1499
TspGWI	ACGGA	2 cut(s)	540, 805
TspRI	CASTG	1 cut(s)	1120
VneI	GTGCAC	1 cut(s)	220
VpaK11BI	GGWCC	4 cut(s)	704, 835, 868, 1721
VspI	ATTAAT	1 cut(s)	99
XagI	CCTNNNNNAGG	1 cut(s)	1654
XapI	RAATTY	3 cut(s)	200, 245, 1645
XbaI	TCTAGA	1 cut(s)	1514
XceI	RCATGY	1 cut(s)	255
XmnI	GAANNNNTTC	1 cut(s)	504
XspI	CTAG	4 cut(s)	447, 846, 1515, 1680
Zsp2I	ATGCAT	2 cut(s)	537, 1428

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.