Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0008187

Physical Location & Seq

Genomic Coordinates Reverse (-)

55282835 .. 55288848

Gene Structure 6014 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G032600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1644 bp

ATGGGGAATTGCTGTGTAACCCTCGGTAACTCTGCCCAGAATAAGAAGAAGACAAAGAAGAAGCCAAATCCCTTTGCTACTGATTTTGGTGCTGCCAATGGCAATGAGGGTGGGGACAAGCTGTGTGTTTTGAAAGACCCGACAGGGAAAGACATTTCGGCCCAATATGATCTGGGTAGGGAGCTCGGCCGTGGAGAATTTGGAATCACTTACCTGTGCACAGATTTGTCATCAGGTGAGAAATTCGCCTGCAAATCGATTTCGAAGAAGAAGCTGAGGACTGCTGTGGATATTGAGGATGTGAGACGTGAGGTGGACATCATGAGGCATTTGCCTCAGCACCCCAACATTGTGTCATTGAAGGACACTTATGAAGATGACGAGGCGGTTCACATTGTGATGGAGTTGTGTGAGGGAGGCGAGCTGTTTGACCGCATTGTTGCTAGGGGTCACTATACGGAGCGTGCAGCTGCTGCTGTAATGCGGACAATTGTTGAAATTGTTCAGAGCTGTCACAAGCATGGAGTAATGCATCGCGATCTCAAACCAGAAAACTTTCTATTTGCAAATAAGAAAGAATCGGCTGCCTTAAAAGCAATTGATTTTGGGTTGTCCGTATTCTTTAAACCTGGTGAGCAATTTAATGAGATAGTCGGAAGTCCGTACTACATGGCTCCAGAGGTTCTGAAGCGCAATTATGGTCCCGAGGTTGACATCTGGAGTGCCGGAGTAATCTTGTATATTTTACTTTGTGGTGTTCCACCTTTCTGGGCAGAAACTGAACAAGGAGTAGCACAAGCAATTATCCGCTCTATCATTGATTTCAAGAGGGACCCTTGGCCTAGAGTCTCTGATAATGCGAAGGACCTGGTAAAAAAAATGCTTGATCCTGATCCCAAGAAGCGGCTGACAGCTCAAGAAGTACTTGATCATCCTTGGATACAAAATGCCAAGAAGGCCCCAAATGTTCCACTTGGTGAGACTGTGAGAGCAAGGCTGAAACAATTTTCTGTAATGAACAAACTAAAAAAAAGAGCTCTGGGGGTCATAGCCGAGCATTTGTCAGTTGAGGAAGTAGCTGGCATAAAGGAGGCATTTCAACTGATGGATACTGATAAAAAAGGCAAGGCTGATGTCGATGGCGATGGAGCTCTGAATTATGGAGAGTTTGTTGCGGTTTCGGTTCACCTTAAAAAGATGGCCAATGATGAGCACCTACATAAAGCTTTTGCATTCTTCGATCAAAACCAAAGTGGTTACATAGAAATTGAAGAGTTGCGAAATGCTTTAAATGATGATGTTGATGCAGGCGGCGAGGAAGTTATCCATGCAATTATGCATGATGTGGATACAGATAAGGATGGGCGCATAAGTTATGAGGAGTTTGTCGCTATGATGAAGTCTGGTACAGATTGGAGAAAAGCATCCAGACAATATTCACGGGAAAGATTCAACAGCATAAGCTTGAAGTTGATGCGTGAAGGGTCTTTACAGTTAACCAATGAGGGCGCCGCCCCTCAGGTGCTGCATGACGGGCTACAAGAGATCCGGCTCGTGCTCGTAGTGCTCAAATGTGGTCTACAGCAAGGACTCATCTCATCCTGGAGGAGGCTTCTTAGGGCAAAGAATATCGAGTTCCATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8 Pkinase

Protein Analysis

547

Amino Acids

61.34

Weight (kDa)

6.62

Isoelectric Point (pI)

37.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	56 - 314	5.6e-80	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	58 - 309	9.1e-26	Protein tyrosine and serine/threonine kinase
EF-hand_7	PF13499	404 - 467	1.2e-15	EF-hand domain pair
EF-hand_6	PF13405	406 - 431	9.2e-06	EF-hand domain
EF-hand_1	PF00036	442 - 468	1.9e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008187)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12480 AT5G12480 AT5G19450 AT5G19450
fragaria_vesca	FvH4_3g28740 FvH4_3g28740 FvH4_3g28740 FvH4_3g28740
malus_domestica	MD03G1173900.v1.1 MD11G1192100.v1.1
prunus_persica	Prupe.4G250200_v2.0.a1 Prupe.4G250200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0052321
rosa_laevigata	RLG00000034825
rosa_multiflora	Rmu_sc0000968.1_g000007 Rmu_sc0028377.1_g000006
rosa_roxburghii	Rroxscaffold_1G00028230
rosa_rugosa	Rorug05G0272800 Rorug05G0272900
rosa_samantha	Rh5AG345400 Rh5CG381800 Rh5DG371600
rosa_wichuraiana	Rw5G032600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1508
AccBSI	CCGCTC	1 cut(s)	810
AccI	GTMKAC	1 cut(s)	1579
AccII	CGCG	1 cut(s)	537
AciI	CCGC	8 cut(s)	386, 433, 484, 808, 904, 1175, 1311, 1512
AclWI	GGATC	3 cut(s)	881, 887, 1540
AcoI	YGGCCR	2 cut(s)	187, 1200
AcsI	RAATTY	2 cut(s)	197, 242
AcuI	CTGAAG	1 cut(s)	707
AcyI	GRCGYC	1 cut(s)	1509
AdeI	CACNNNGTG	2 cut(s)	397, 977
AfaI	GTAC	3 cut(s)	665, 924, 1408
AfiI	CCNNNNNNNGG	2 cut(s)	903, 1608
AgsI	TTSAA	8 cut(s)	133, 361, 497, 826, 1100, 1271, 1453, 1468
AjiI	CACGTC	1 cut(s)	308
AjnI	CCWGG	3 cut(s)	628, 867, 1601
Alw21I	GWGCWC	7 cut(s)	186, 221, 1039, 1153, 1215, 1560, 1569
Alw26I	GTCTC	3 cut(s)	298, 853, 974
Alw44I	GTGCAC	1 cut(s)	217
AlwI	GGATC	3 cut(s)	881, 887, 1540
AlwNI	CAGNNNCTG	3 cut(s)	473, 779, 1525
Ama87I	CYCGRG	1 cut(s)	704
AoxI	GGCC	5 cut(s)	159, 187, 839, 957, 1200
ApaLI	GTGCAC	1 cut(s)	217
ApeKI	GCWGC	6 cut(s)	92, 467, 470, 473, 584, 1525
ApoI	RAATTY	2 cut(s)	197, 242
Asp700I	GAANNNNTTC	2 cut(s)	501, 555
AspLEI	GCGC	3 cut(s)	693, 1368, 1511
AspS9I	GGNCC	5 cut(s)	160, 701, 832, 865, 958
AsuHPI	GGTGA	4 cut(s)	248, 644, 989, 1178
AsuII	TTCGAA	1 cut(s)	263
AvaI	CYCGRG	1 cut(s)	704
AvaII	GGWCC	3 cut(s)	701, 832, 865
AxyI	CCTNAGG	1 cut(s)	1518
BaeGI	GKGCMC	1 cut(s)	221
BalI	TGGCCA	1 cut(s)	1202
BanI	GGYRCC	1 cut(s)	1508
BanII	GRGCYC	3 cut(s)	186, 1039, 1153
BarI	GAAGNNNNNNTAC	2 cut(s)	1473, 1505
BauI	CACGAG	1 cut(s)	1553
BbsI	GAAGAC	1 cut(s)	56
Bbv12I	GWGCWC	7 cut(s)	186, 221, 1039, 1153, 1215, 1560, 1569
BbvCI	CCTCAGC	2 cut(s)	275, 336
BbvI	GCAGC	6 cut(s)	79, 457, 460, 479, 571, 1512
BccI	CCATC	6 cut(s)	394, 1099, 1133, 1139, 1192, 1355
BceAI	ACGGC	1 cut(s)	174
BciT130I	CCWGG	3 cut(s)	630, 869, 1603
BciVI	GTATCC	3 cut(s)	933, 1102, 1342
BclI	TGATCA	1 cut(s)	928
BcoDI	GTCTC	3 cut(s)	298, 853, 974
BfaI	CTAG	2 cut(s)	444, 843
BfmI	CTRYAG	1 cut(s)	1580
BfoI	RGCGCY	1 cut(s)	1512
BfuI	GTATCC	3 cut(s)	933, 1102, 1342
BglI	GCCNNNNNGGC	1 cut(s)	956
BisI	GCNGC	9 cut(s)	93, 468, 471, 474, 585, 905, 1312, 1512, 1526
BlsI	GCNGC	9 cut(s)	94, 469, 472, 475, 586, 906, 1313, 1513, 1527
BmcAI	AGTACT	1 cut(s)	924
Bme1390I	CCNGG	3 cut(s)	630, 869, 1603
Bme18I	GGWCC	3 cut(s)	701, 832, 865
BmeT110I	CYCGRG	1 cut(s)	704
BmgBI	CACGTC	1 cut(s)	308
BmgT120I	GGNCC	5 cut(s)	160, 701, 832, 865, 958
BmiI	GGNNCC	6 cut(s)	675, 703, 833, 834, 960, 1510
BmrFI	CCNGG	3 cut(s)	630, 869, 1603
BmsI	GCATC	4 cut(s)	541, 1294, 1433, 1464
BpiI	GAAGAC	1 cut(s)	56
BpmI	CTGGAG	3 cut(s)	660, 739, 1624
Bpu10I	CCTNAGC	2 cut(s)	275, 336
Bpu14I	TTCGAA	1 cut(s)	263
BpuEI	CTTGAG	1 cut(s)	900
Bsa29I	ATCGAT	1 cut(s)	257
BsaBI	GATNNNNATC	1 cut(s)	891
BsaHI	GRCGYC	1 cut(s)	1509
BsaJI	CCNNGG	5 cut(s)	22, 190, 705, 836, 935
BsaXI	ACNNNNNCTCC	2 cut(s)	1155, 1185
Bsc4I	CCNNNNNNNGG	2 cut(s)	903, 1608
Bse21I	CCTNAGG	1 cut(s)	1518
Bse3DI	GCAATG	1 cut(s)	109
Bse8I	GATNNNNATC	1 cut(s)	891
BseBI	CCWGG	3 cut(s)	630, 869, 1603
BseCI	ATCGAT	1 cut(s)	257
BseDI	CCNNGG	5 cut(s)	22, 190, 705, 836, 935
BseGI	GGATG	5 cut(s)	304, 931, 1366, 1424, 1598
BseJI	GATNNNNATC	1 cut(s)	891
BseLI	CCNNNNNNNGG	2 cut(s)	903, 1608
BseMI	GCAATG	1 cut(s)	109
BseMII	CTCAG	3 cut(s)	266, 350, 1532
BseRI	GAGGAG	2 cut(s)	1394, 1621
BseSI	GKGCMC	1 cut(s)	221
BseX3I	CGGCCG	1 cut(s)	187
BseXI	GCAGC	6 cut(s)	79, 457, 460, 479, 571, 1512
BsgI	GTGCAG	1 cut(s)	486
Bsh1236I	CGCG	1 cut(s)	537
Bsh1285I	CGRYCG	1 cut(s)	190
BshFI	GGCC	5 cut(s)	161, 189, 841, 959, 1202
BshNI	GGYRCC	1 cut(s)	1508
BshVI	ATCGAT	1 cut(s)	257
BsiEI	CGRYCG	1 cut(s)	190
BsiHKAI	GWGCWC	7 cut(s)	186, 221, 1039, 1153, 1215, 1560, 1569
BsiHKCI	CYCGRG	1 cut(s)	704
BsiSI	CCGG	2 cut(s)	726, 1549
BslFI	GGGAC	3 cut(s)	128, 687, 845
BslI	CCNNNNNNNGG	2 cut(s)	903, 1608
BsmAI	GTCTC	3 cut(s)	298, 853, 974
BsmBI	CGTCTC	1 cut(s)	298
BsmFI	GGGAC	3 cut(s)	128, 687, 845
BsmI	GAATGC	1 cut(s)	1232
BsnI	GGCC	5 cut(s)	161, 189, 841, 959, 1202
BsoBI	CYCGRG	1 cut(s)	704
Bsp119I	TTCGAA	1 cut(s)	263
Bsp1286I	GDGCHC	7 cut(s)	186, 221, 1039, 1153, 1215, 1560, 1569
Bsp143I	GATC	7 cut(s)	169, 538, 886, 892, 928, 1240, 1545
Bsp68I	TCGCGA	1 cut(s)	537
BspACI	CCGC	8 cut(s)	386, 433, 484, 808, 904, 1175, 1311, 1512
BspANI	GGCC	5 cut(s)	161, 189, 841, 959, 1202
BspCNI	CTCAG	3 cut(s)	267, 349, 1531
BspDI	ATCGAT	1 cut(s)	257
BspFNI	CGCG	1 cut(s)	537
BspHI	TCATGA	1 cut(s)	321
BspLI	GGNNCC	6 cut(s)	675, 703, 833, 834, 960, 1510
BspPI	GGATC	3 cut(s)	881, 887, 1540
BspT104I	TTCGAA	1 cut(s)	263
BspT107I	GGYRCC	1 cut(s)	1508
BsrBI	CCGCTC	1 cut(s)	810
BsrDI	GCAATG	1 cut(s)	109
BssECI	CCNNGG	5 cut(s)	22, 190, 705, 836, 935
BssMI	GATC	7 cut(s)	169, 538, 886, 892, 928, 1240, 1545
BssNI	GRCGYC	1 cut(s)	1509
BssSI	CACGAG	1 cut(s)	1553
BssT1I	CCWWGG	2 cut(s)	836, 935
Bst2BI	CACGAG	1 cut(s)	1553
Bst2UI	CCWGG	3 cut(s)	630, 869, 1603
Bst4CI	ACNGT	2 cut(s)	985, 1494
Bst6I	CTCTTC	1 cut(s)	1266
BstACI	GRCGYC	1 cut(s)	1509
BstAPI	GCANNNNNTGC	1 cut(s)	473
BstBI	TTCGAA	1 cut(s)	263
BstC8I	GCNNGC	5 cut(s)	250, 422, 465, 1081, 1309
BstDEI	CTNAG	4 cut(s)	275, 336, 1518, 1616
BstDSI	CCRYGG	1 cut(s)	190
BstENI	CCTNNNNNAGG	1 cut(s)	1606
BstF5I	GGATG	5 cut(s)	304, 931, 1366, 1424, 1598
BstFNI	CGCG	1 cut(s)	537
BstH2I	RGCGCY	1 cut(s)	1512
BstHHI	GCGC	3 cut(s)	693, 1368, 1511
BstKTI	GATC	7 cut(s)	172, 541, 889, 895, 931, 1243, 1548
BstMAI	GTCTC	3 cut(s)	298, 853, 974
BstMBI	GATC	7 cut(s)	169, 538, 886, 892, 928, 1240, 1545
BstMCI	CGRYCG	1 cut(s)	190
BstMWI	GCNNNNNNNGC	5 cut(s)	473, 593, 956, 1534, 1564
BstNI	CCWGG	3 cut(s)	630, 869, 1603
BstSCI	CCNGG	3 cut(s)	628, 867, 1601
BstSFI	CTRYAG	1 cut(s)	1580
BstSLI	GKGCMC	1 cut(s)	221
BstUI	CGCG	1 cut(s)	537
BstV1I	GCAGC	6 cut(s)	79, 457, 460, 479, 571, 1512
BstV2I	GAAGAC	1 cut(s)	56
BstX2I	RGATCY	1 cut(s)	1545
BstXI	CCANNNNNNTGG	1 cut(s)	768
BstYI	RGATCY	1 cut(s)	1545
BstZI	CGGCCG	1 cut(s)	187
Bsu15I	ATCGAT	1 cut(s)	257
Bsu36I	CCTNAGG	1 cut(s)	1518
BsuI	GTATCC	3 cut(s)	933, 1102, 1342
BsuRI	GGCC	5 cut(s)	161, 189, 841, 959, 1202
BsuTUI	ATCGAT	1 cut(s)	257
BtgI	CCRYGG	1 cut(s)	190
BtgZI	GCGATG	2 cut(s)	518, 1158
BtrI	CACGTC	1 cut(s)	308
BtsCI	GGATG	5 cut(s)	304, 931, 1366, 1424, 1598
BtuMI	TCGCGA	1 cut(s)	537
Cac8I	GCNNGC	5 cut(s)	250, 422, 465, 1081, 1309
CaiI	CAGNNNCTG	3 cut(s)	473, 779, 1525
CciI	TCATGA	1 cut(s)	321
CfoI	GCGC	3 cut(s)	693, 1368, 1511
Cfr13I	GGNCC	5 cut(s)	160, 701, 832, 865, 958
ClaI	ATCGAT	1 cut(s)	257
CsiI	ACCWGGT	2 cut(s)	628, 867
Csp6I	GTAC	3 cut(s)	664, 923, 1407
CspCI	CAANNNNNGTGG	2 cut(s)	91, 126
CviAII	CATG	6 cut(s)	322, 521, 670, 1328, 1340, 1529
CviQI	GTAC	3 cut(s)	664, 923, 1407
DdeI	CTNAG	4 cut(s)	275, 336, 1518, 1616
DinI	GGCGCC	1 cut(s)	1510
DpnI	GATC	7 cut(s)	171, 540, 888, 894, 930, 1242, 1547
DpnII	GATC	7 cut(s)	169, 538, 886, 892, 928, 1240, 1545
DraI	TTTAAA	2 cut(s)	625, 1290
DraIII	CACNNNGTG	2 cut(s)	397, 977
EaeI	YGGCCR	2 cut(s)	187, 1200
EagI	CGGCCG	1 cut(s)	187
Eam1104I	CTCTTC	1 cut(s)	1266
EarI	CTCTTC	1 cut(s)	1266
Ecl136II	GAGCTC	3 cut(s)	184, 1037, 1151
EclXI	CGGCCG	1 cut(s)	187
Eco130I	CCWWGG	2 cut(s)	836, 935
Eco24I	GRGCYC	3 cut(s)	186, 1039, 1153
Eco47I	GGWCC	3 cut(s)	701, 832, 865
Eco52I	CGGCCG	1 cut(s)	187
Eco53kI	GAGCTC	3 cut(s)	184, 1037, 1151
Eco57I	CTGAAG	1 cut(s)	707
Eco81I	CCTNAGG	1 cut(s)	1518
Eco88I	CYCGRG	1 cut(s)	704
EcoICRI	GAGCTC	3 cut(s)	184, 1037, 1151
EcoNI	CCTNNNNNAGG	1 cut(s)	1606
EcoO109I	RGGNCCY	3 cut(s)	832, 865, 958
EcoRII	CCWGG	3 cut(s)	628, 867, 1601
EcoT14I	CCWWGG	2 cut(s)	836, 935
EcoT22I	ATGCAT	2 cut(s)	534, 1341
EcoT38I	GRGCYC	3 cut(s)	186, 1039, 1153
EgeI	GGCGCC	1 cut(s)	1510
EheI	GGCGCC	1 cut(s)	1510
ErhI	CCWWGG	2 cut(s)	836, 935
Esp3I	CGTCTC	1 cut(s)	298
FaeI	CATG	6 cut(s)	325, 524, 673, 1331, 1343, 1532
FalI	AAGNNNNNCTT	2 cut(s)	909, 941
FaqI	GGGAC	3 cut(s)	128, 687, 845
FatI	CATG	6 cut(s)	321, 520, 669, 1327, 1339, 1528
FbaI	TGATCA	1 cut(s)	928
FblI	GTMKAC	1 cut(s)	1579
Fnu4HI	GCNGC	9 cut(s)	93, 468, 471, 474, 585, 905, 1312, 1512, 1526
FokI	GGATG	5 cut(s)	311, 918, 1373, 1411, 1585
FriOI	GRGCYC	3 cut(s)	186, 1039, 1153
Fsp4HI	GCNGC	9 cut(s)	93, 468, 471, 474, 585, 905, 1312, 1512, 1526
FspBI	CTAG	2 cut(s)	444, 843
GlaI	GCGC	3 cut(s)	692, 1367, 1510
GluI	GCNGC	9 cut(s)	93, 468, 471, 474, 585, 905, 1312, 1512, 1526
GsuI	CTGGAG	3 cut(s)	660, 739, 1624
HaeII	RGCGCY	1 cut(s)	1512
HaeIII	GGCC	5 cut(s)	161, 189, 841, 959, 1202
HapII	CCGG	2 cut(s)	726, 1549
HhaI	GCGC	3 cut(s)	693, 1368, 1511
Hin1I	GRCGYC	1 cut(s)	1509
Hin1II	CATG	6 cut(s)	325, 524, 673, 1331, 1343, 1532
Hin6I	GCGC	3 cut(s)	691, 1366, 1509
HinP1I	GCGC	3 cut(s)	691, 1366, 1509
HincII	GTYRAC	2 cut(s)	712, 1497
HindII	GTYRAC	2 cut(s)	712, 1497
HindIII	AAGCTT	2 cut(s)	1224, 1462
HinfI	GANTC	5 cut(s)	204, 578, 846, 1449, 1590
HpaI	GTTAAC	1 cut(s)	1497
HpaII	CCGG	2 cut(s)	726, 1549
HphI	GGTGA	4 cut(s)	248, 644, 989, 1178
Hpy166II	GTNNAC	7 cut(s)	219, 316, 391, 712, 1186, 1497, 1580
Hpy188I	TCNGA	5 cut(s)	507, 656, 687, 853, 1155
Hpy188III	TCNNGA	9 cut(s)	322, 536, 677, 704, 718, 826, 890, 917, 1428
Hpy8I	GTNNAC	7 cut(s)	219, 316, 391, 712, 1186, 1497, 1580
HpyAV	CCTTC	4 cut(s)	355, 856, 949, 1475
HpyCH4III	ACNGT	2 cut(s)	985, 1494
HpyCH4IV	ACGT	1 cut(s)	307
HpyF10VI	GCNNNNNNNGC	5 cut(s)	473, 593, 956, 1534, 1564
HpyF3I	CTNAG	4 cut(s)	275, 336, 1518, 1616
HpySE526I	ACGT	1 cut(s)	307
Hsp92I	GRCGYC	1 cut(s)	1509
Hsp92II	CATG	6 cut(s)	325, 524, 673, 1331, 1343, 1532
HspAI	GCGC	3 cut(s)	691, 1366, 1509
KasI	GGCGCC	1 cut(s)	1508
KflI	GGGWCCC	1 cut(s)	832
Ksp22I	TGATCA	1 cut(s)	928
KspAI	GTTAAC	1 cut(s)	1497
Kzo9I	GATC	7 cut(s)	169, 538, 886, 892, 928, 1240, 1545
LmnI	GCTCC	4 cut(s)	181, 460, 679, 1148
Lsp1109I	GCAGC	6 cut(s)	79, 457, 460, 479, 571, 1512
LweI	GCATC	4 cut(s)	541, 1294, 1433, 1464
MabI	ACCWGGT	2 cut(s)	628, 867
MaeI	CTAG	2 cut(s)	444, 843
MaeII	ACGT	1 cut(s)	307
MaeIII	GTNAC	5 cut(s)	16, 26, 449, 512, 1256
MalI	GATC	7 cut(s)	171, 540, 888, 894, 930, 1242, 1547
MbiI	CCGCTC	1 cut(s)	810
MboI	GATC	7 cut(s)	169, 538, 886, 892, 928, 1240, 1545
MboII	GAAGA	8 cut(s)	58, 61, 70, 277, 280, 386, 1228, 1283
MfeI	CAATTG	2 cut(s)	489, 597
MflI	RGATCY	1 cut(s)	1545
MhlI	GDGCHC	7 cut(s)	186, 221, 1039, 1153, 1215, 1560, 1569
MlsI	TGGCCA	1 cut(s)	1202
MluNI	TGGCCA	1 cut(s)	1202
Mly113I	GGCGCC	1 cut(s)	1509
MlyI	GAGTC	2 cut(s)	855, 1584
MmeI	TCCRAC	1 cut(s)	634
Mox20I	TGGCCA	1 cut(s)	1202
Mph1103I	ATGCAT	2 cut(s)	534, 1341
MroXI	GAANNNNTTC	2 cut(s)	501, 555
MscI	TGGCCA	1 cut(s)	1202
MseI	TTAA	7 cut(s)	590, 624, 642, 1191, 1289, 1496, 1642
MslI	CAYNNNNRTG	2 cut(s)	398, 519
Msp20I	TGGCCA	1 cut(s)	1202
MspA1I	CMGCKG	1 cut(s)	470
MspI	CCGG	2 cut(s)	726, 1549
MspR9I	CCNGG	3 cut(s)	630, 869, 1603
MunI	CAATTG	2 cut(s)	489, 597
Mva1269I	GAATGC	1 cut(s)	1232
MvaI	CCWGG	3 cut(s)	630, 869, 1603
MvnI	CGCG	1 cut(s)	537
MwoI	GCNNNNNNNGC	5 cut(s)	473, 593, 956, 1534, 1564
NarI	GGCGCC	1 cut(s)	1509
NdeII	GATC	7 cut(s)	169, 538, 886, 892, 928, 1240, 1545
NlaIII	CATG	6 cut(s)	325, 524, 673, 1331, 1343, 1532
NlaIV	GGNNCC	6 cut(s)	675, 703, 833, 834, 960, 1510
NmeAIII	GCCGAG	2 cut(s)	165, 1078
NmuCI	GTSAC	2 cut(s)	449, 512
NruI	TCGCGA	1 cut(s)	537
NsiI	ATGCAT	2 cut(s)	534, 1341
NspV	TTCGAA	1 cut(s)	263
PagI	TCATGA	1 cut(s)	321
PctI	GAATGC	1 cut(s)	1232
PdmI	GAANNNNTTC	2 cut(s)	501, 555
PfeI	GAWTC	3 cut(s)	204, 578, 1449
PfoI	TCCNGGA	1 cut(s)	1601
PkrI	GCNGC	9 cut(s)	94, 469, 472, 475, 586, 906, 1313, 1513, 1527
PleI	GAGTC	2 cut(s)	854, 1584
PluTI	GGCGCC	1 cut(s)	1512
PpsI	GAGTC	2 cut(s)	854, 1584
PpuMI	RGGWCCY	2 cut(s)	832, 865
Psp124BI	GAGCTC	3 cut(s)	186, 1039, 1153
Psp5II	RGGWCCY	2 cut(s)	832, 865
Psp6I	CCWGG	3 cut(s)	628, 867, 1601
PspGI	CCWGG	3 cut(s)	628, 867, 1601
PspN4I	GGNNCC	6 cut(s)	675, 703, 833, 834, 960, 1510
PspPI	GGNCC	5 cut(s)	160, 701, 832, 865, 958
PspPPI	RGGWCCY	2 cut(s)	832, 865
PstNI	CAGNNNCTG	3 cut(s)	473, 779, 1525
PsuI	RGATCY	1 cut(s)	1545
PvuII	CAGCTG	1 cut(s)	470
RruI	TCGCGA	1 cut(s)	537
RsaI	GTAC	3 cut(s)	665, 924, 1408
RsaNI	GTAC	3 cut(s)	664, 923, 1407
RseI	CAYNNNNRTG	2 cut(s)	398, 519
SacI	GAGCTC	3 cut(s)	186, 1039, 1153
SaqAI	TTAA	7 cut(s)	590, 624, 642, 1191, 1289, 1496, 1642
SatI	GCNGC	9 cut(s)	93, 468, 471, 474, 585, 905, 1312, 1512, 1526
Sau3AI	GATC	7 cut(s)	169, 538, 886, 892, 928, 1240, 1545
Sau96I	GGNCC	5 cut(s)	160, 701, 832, 865, 958
ScaI	AGTACT	1 cut(s)	924
SchI	GAGTC	2 cut(s)	855, 1584
ScrFI	CCNGG	3 cut(s)	630, 869, 1603
SduI	GDGCHC	7 cut(s)	186, 221, 1039, 1153, 1215, 1560, 1569
SexAI	ACCWGGT	2 cut(s)	628, 867
SfaNI	GCATC	4 cut(s)	541, 1294, 1433, 1464
SfcI	CTRYAG	1 cut(s)	1580
SfoI	GGCGCC	1 cut(s)	1510
SfuI	TTCGAA	1 cut(s)	263
SinI	GGWCC	3 cut(s)	701, 832, 865
SmiMI	CAYNNNNRTG	2 cut(s)	398, 519
SmlI	CTYRAG	1 cut(s)	915
SmoI	CTYRAG	1 cut(s)	915
SsiI	CCGC	8 cut(s)	386, 433, 484, 808, 904, 1175, 1311, 1512
SspDI	GGCGCC	1 cut(s)	1508
SspI	AATATT	1 cut(s)	1436
SspMI	CTAG	2 cut(s)	444, 843
SstI	GAGCTC	3 cut(s)	186, 1039, 1153
StyD4I	CCNGG	3 cut(s)	628, 867, 1601
StyI	CCWWGG	2 cut(s)	836, 935
TaaI	ACNGT	2 cut(s)	985, 1494
TaiI	ACGT	1 cut(s)	310
TaqI	TCGA	5 cut(s)	257, 263, 1137, 1239, 1632
TatI	WGTACW	1 cut(s)	922
TauI	GCSGC	3 cut(s)	907, 1314, 1514
TfiI	GAWTC	3 cut(s)	204, 578, 1449
Tru1I	TTAA	7 cut(s)	590, 624, 642, 1191, 1289, 1496, 1642
Tru9I	TTAA	7 cut(s)	590, 624, 642, 1191, 1289, 1496, 1642
TseFI	GTSAC	2 cut(s)	449, 512
TseI	GCWGC	6 cut(s)	92, 467, 470, 473, 584, 1525
Tsp45I	GTSAC	2 cut(s)	449, 512
TspDTI	ATGAA	3 cut(s)	387, 1031, 1412
TspGWI	ACGGA	3 cut(s)	473, 604, 651
VneI	GTGCAC	1 cut(s)	217
VpaK11BI	GGWCC	3 cut(s)	701, 832, 865
XagI	CCTNNNNNAGG	1 cut(s)	1606
XapI	RAATTY	2 cut(s)	197, 242
XmiI	GTMKAC	1 cut(s)	1579
XmnI	GAANNNNTTC	2 cut(s)	501, 555
XspI	CTAG	2 cut(s)	444, 843
ZrmI	AGTACT	1 cut(s)	924
Zsp2I	ATGCAT	2 cut(s)	534, 1341

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.