Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0008187

Physical Location & Seq

Genomic Coordinates Reverse (-)

27301712 .. 27306030

Gene Structure 4319 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1192100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1602 bp

ATGGGAAATTGTTGTGTAACTCTCGGCACATCAGGCCAGAACAAGCAGAAGAAAAACAACAAGAAGACAAACCCATTTGCAACAGATTTTGGTGGCATTAATGGAAAAGATGGTGATGGGAAACTGTGTGTCTTGAAAGACCCTACAGGTCGAGACATTTCGACCCAATATGACCTGGGACGCGAGCTTGGCCGAGGCGAATTCGGAGTAACATATCTGTGCACAGAGGCATCCTCAAACGAAAAGTTTGCTTGCAAATCAATATCGAAGAAGAAGCTGAGGACTGCAGTGGATATTGAGGATGTGAGGAGGGAAGTTGAGATAATGCGGCATTTGCCTCCACACCCAAATATTGTGACATTGAAGGACACTTATGAGGATGATGATGCGATTCACATTGTGATGGAGTTGTGCGAGGGTGGTGAGTTGTTCGACCGCATTGTTGCTCGGGGTCATTACACAGAACGGGCCGCTGCAGGCGTAATGAGGACGATTGTTGAAGTTGTCCAGATGTGTCATAAACACGGAGTAATGCATCGGGATCTTAAACCAGAGAACTTCTTATTTGCAAATAAGAAGGAAACGGCCTCTTTGAAATCAATAGATTTTGGATTGTCTGTGTTCTTCAAACCTGGTGAGCAATTTAATGAGATAGTGGGAAGTCCATATTACATGGCTCCAGAGGTTCTGAAGCGCAACTATGGACCTGAGGTTGACATCTGGAGTGCTGGGGTTATCCTATATATTTTACTTTGTGGCGTTCCACCTTTCTGGGCAGAAACTGAACAAGGGGTAGCCCAAGCAATTATTCGATCCGTCATTGACTTCAAGAGGGACCCCTGGCCTAGAGTGTCAGATAATGCAAAGGACCTCGTGAAGAAAATGCTAAATCCTGATCCAAAGAAGCGGTTAACAGCTCAGGAAGTGCTTGATCATCATTGGATTCAAAATGCCAAGAAGGCCCCAAATGTCCCACTTGGTGAGACTGTGAGAGCAAGGTTGAAGCAATTTTCTGTAATGAACAAGCTGAAGAAAAGAGCTCTAGGGGTCATAGCTGACCATTTGTCAGTTGAGGAAGTTGCTGGCATAAAAGAGGCTTTTCAAATGATGGATGCTGGCAACAAGGGCAAGGTAACCATAGAAGAGCTACGAAGTGGGATACAACAGCTGGGGCAAAATATTCCTGATCCAGATCTTCAAATACTAATGGAAGCGGCTGATGTCGATGGGGATGGAGCTCTCAACTATGGAGAGTTTGTTGCTGTTACGGTTCACCTTAAAAGAATGGCAAATGACGAGCATCTGCATAAAGCCTTTTCATTCTTCGACCAAAACCAAAGTGGTTACATAGAAATTGAAGAATTAAGAAATGCTTTAACTGATGATGTTGACACAGGTGGTGAGGAAGTCATCCATGCAATTATGCATGACGTAGACACAGATAAGGATGGGCGCATCAGTTACGAGGAGTTTGCTGCTATGATGAAGGCTGGTACAGATTGGAGAAAAGCATCCAGGCAATATTCACGGGAAAGATTCAACAGCATAAGCTTGAAGTTGATGAGAGAAGGGTCTTTACAGTTAGCCAATGAGGGTAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8 Pkinase

Protein Analysis

534

Amino Acids

59.79

Weight (kDa)

6.06

Isoelectric Point (pI)

36.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	57 - 315	4.9e-81	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	59 - 310	1.5e-26	Protein tyrosine and serine/threonine kinase
EF-hand_7	PF13499	363 - 422	4.7e-11	EF-hand domain pair
EF-hand_7	PF13499	433 - 496	6.8e-16	EF-hand domain pair
EF-hand_1	PF00036	435 - 460	8.3e-06	EF hand domain
EF-hand_6	PF13405	435 - 460	4.9e-06	EF-hand domain
EF-hand_1	PF00036	471 - 497	6.4e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008187)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12480 AT5G12480 AT5G19450 AT5G19450
fragaria_vesca	FvH4_3g28740 FvH4_3g28740 FvH4_3g28740 FvH4_3g28740
malus_domestica	MD03G1173900.v1.1 MD11G1192100.v1.1
prunus_persica	Prupe.4G250200_v2.0.a1 Prupe.4G250200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0052321
rosa_laevigata	RLG00000034825
rosa_multiflora	Rmu_sc0000968.1_g000007 Rmu_sc0028377.1_g000006
rosa_roxburghii	Rroxscaffold_1G00028230
rosa_rugosa	Rorug05G0272800 Rorug05G0272900
rosa_samantha	Rh5AG345400 Rh5CG381800 Rh5DG371600
rosa_wichuraiana	Rw5G032600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	1434
AccII	CGCG	1 cut(s)	183
AciI	CCGC	5 cut(s)	328, 436, 471, 907, 1214
AclWI	GGATC	4 cut(s)	549, 807, 890, 1181
AcoI	YGGCCR	1 cut(s)	190
AcsI	RAATTY	1 cut(s)	200
AcuI	CTGAAG	2 cut(s)	710, 1049
AdeI	CACNNNGTG	2 cut(s)	400, 980
AfaI	GTAC	1 cut(s)	1495
AhdI	GACNNNNNGTC	1 cut(s)	1063
AjnI	CCWGG	4 cut(s)	174, 631, 839, 1514
AjuI	GAANNNNNNNTTGG	2 cut(s)	792, 824
Alw21I	GWGCWC	3 cut(s)	224, 1042, 1240
Alw26I	GTCTC	2 cut(s)	147, 977
Alw44I	GTGCAC	1 cut(s)	220
AlwI	GGATC	4 cut(s)	549, 807, 890, 1181
AlwNI	CAGNNNCTG	1 cut(s)	782
Ama87I	CYCGRG	1 cut(s)	447
AoxI	GGCC	6 cut(s)	34, 190, 468, 585, 842, 960
ApaLI	GTGCAC	1 cut(s)	220
ApeKI	GCWGC	2 cut(s)	473, 1475
ApoI	RAATTY	1 cut(s)	200
AseI	ATTAAT	1 cut(s)	99
AspLEI	GCGC	2 cut(s)	696, 1455
AspS9I	GGNCC	5 cut(s)	468, 704, 835, 868, 961
AsuHPI	GGTGA	6 cut(s)	125, 434, 647, 992, 1265, 1412
AvaI	CYCGRG	1 cut(s)	447
AvaII	GGWCC	3 cut(s)	704, 835, 868
AxyI	CCTNAGG	1 cut(s)	708
BaeGI	GKGCMC	1 cut(s)	224
BanII	GRGCYC	2 cut(s)	1042, 1240
BarI	GAAGNNNNNNTAC	2 cut(s)	1560, 1592
BauI	CACGAG	1 cut(s)	872
BbsI	GAAGAC	1 cut(s)	71
Bbv12I	GWGCWC	3 cut(s)	224, 1042, 1240
BbvCI	CCTCAGC	1 cut(s)	278
BbvI	GCAGC	2 cut(s)	460, 1462
BccI	CCATC	7 cut(s)	104, 110, 397, 1102, 1220, 1226, 1442
BceAI	ACGGC	1 cut(s)	600
BcgI	CGANNNNNNTGC	4 cut(s)	230, 264, 1454, 1488
BciT130I	CCWGG	4 cut(s)	176, 633, 841, 1516
BciVI	GTATCC	1 cut(s)	1152
BclI	TGATCA	1 cut(s)	931
BcoDI	GTCTC	2 cut(s)	147, 977
BfaI	CTAG	2 cut(s)	846, 1043
BfmI	CTRYAG	3 cut(s)	144, 285, 474
BfuI	GTATCC	1 cut(s)	1152
BglI	GCCNNNNNGGC	1 cut(s)	959
BglII	AGATCT	1 cut(s)	1192
BisI	GCNGC	5 cut(s)	329, 471, 474, 1215, 1476
BlsI	GCNGC	5 cut(s)	330, 472, 475, 1216, 1477
Bme1390I	CCNGG	4 cut(s)	176, 633, 841, 1516
Bme18I	GGWCC	3 cut(s)	704, 835, 868
BmeRI	GACNNNNNGTC	1 cut(s)	1063
BmeT110I	CYCGRG	1 cut(s)	447
BmgT120I	GGNCC	5 cut(s)	468, 704, 835, 868, 961
BmiI	GGNNCC	4 cut(s)	678, 836, 837, 963
BmrFI	CCNGG	4 cut(s)	176, 633, 841, 1516
BmsI	GCATC	7 cut(s)	239, 376, 544, 1102, 1309, 1464, 1520
BpiI	GAAGAC	1 cut(s)	71
BplI	GAGNNNNNCTC	2 cut(s)	218, 250
BpmI	CTGGAG	2 cut(s)	663, 742
Bpu10I	CCTNAGC	2 cut(s)	278, 918
BsaBI	GATNNNNATC	1 cut(s)	1191
BsaJI	CCNNGG	3 cut(s)	175, 193, 839
BsaXI	ACNNNNNCTCC	2 cut(s)	1242, 1272
Bse21I	CCTNAGG	1 cut(s)	708
Bse8I	GATNNNNATC	1 cut(s)	1191
BseBI	CCWGG	4 cut(s)	176, 633, 841, 1516
BseDI	CCNNGG	3 cut(s)	175, 193, 839
BseGI	GGATG	8 cut(s)	230, 307, 385, 1117, 1237, 1410, 1453, 1511
BseJI	GATNNNNATC	1 cut(s)	1191
BseMII	CTCAG	3 cut(s)	269, 699, 932
BseRI	GAGGAG	2 cut(s)	322, 1481
BseSI	GKGCMC	1 cut(s)	224
BseXI	GCAGC	2 cut(s)	460, 1462
BseYI	CCCAGC	2 cut(s)	728, 1168
Bsh1236I	CGCG	1 cut(s)	183
Bsh1285I	CGRYCG	1 cut(s)	436
BshFI	GGCC	6 cut(s)	36, 192, 470, 587, 844, 962
BsiEI	CGRYCG	1 cut(s)	436
BsiHKAI	GWGCWC	3 cut(s)	224, 1042, 1240
BsiHKCI	CYCGRG	1 cut(s)	447
BslFI	GGGAC	3 cut(s)	192, 848, 956
BsmAI	GTCTC	2 cut(s)	147, 977
BsmFI	GGGAC	3 cut(s)	192, 848, 956
BsnI	GGCC	6 cut(s)	36, 192, 470, 587, 844, 962
BsoBI	CYCGRG	1 cut(s)	447
Bsp1286I	GDGCHC	3 cut(s)	224, 1042, 1240
Bsp143I	GATC	6 cut(s)	541, 812, 895, 931, 1186, 1192
BspACI	CCGC	5 cut(s)	328, 436, 471, 907, 1214
BspANI	GGCC	6 cut(s)	36, 192, 470, 587, 844, 962
BspCNI	CTCAG	3 cut(s)	270, 700, 931
BspFNI	CGCG	1 cut(s)	183
BspLI	GGNNCC	4 cut(s)	678, 836, 837, 963
BspMAI	CTGCAG	2 cut(s)	289, 478
BspPI	GGATC	4 cut(s)	549, 807, 890, 1181
BspQI	GCTCTTC	1 cut(s)	1137
BssECI	CCNNGG	3 cut(s)	175, 193, 839
BssMI	GATC	6 cut(s)	541, 812, 895, 931, 1186, 1192
BssSI	CACGAG	1 cut(s)	872
Bst2BI	CACGAG	1 cut(s)	872
Bst2UI	CCWGG	4 cut(s)	176, 633, 841, 1516
Bst4CI	ACNGT	4 cut(s)	126, 988, 1270, 1581
Bst6I	CTCTTC	1 cut(s)	1137
BstC8I	GCNNGC	5 cut(s)	185, 253, 478, 1084, 1117
BstDEI	CTNAG	3 cut(s)	278, 708, 918
BstEII	GGTNACC	1 cut(s)	1132
BstF5I	GGATG	8 cut(s)	230, 307, 385, 1117, 1237, 1410, 1453, 1511
BstFNI	CGCG	1 cut(s)	183
BstHHI	GCGC	2 cut(s)	696, 1455
BstKTI	GATC	6 cut(s)	544, 815, 898, 934, 1189, 1195
BstMAI	GTCTC	2 cut(s)	147, 977
BstMBI	GATC	6 cut(s)	541, 812, 895, 931, 1186, 1192
BstMCI	CGRYCG	1 cut(s)	436
BstMWI	GCNNNNNNNGC	5 cut(s)	33, 189, 334, 959, 1125
BstNI	CCWGG	4 cut(s)	176, 633, 841, 1516
BstPI	GGTNACC	1 cut(s)	1132
BstSCI	CCNGG	4 cut(s)	174, 631, 839, 1514
BstSFI	CTRYAG	3 cut(s)	144, 285, 474
BstSLI	GKGCMC	1 cut(s)	224
BstUI	CGCG	1 cut(s)	183
BstV1I	GCAGC	2 cut(s)	460, 1462
BstV2I	GAAGAC	1 cut(s)	71
BstX2I	RGATCY	2 cut(s)	541, 1192
BstXI	CCANNNNNNTGG	1 cut(s)	771
BstYI	RGATCY	2 cut(s)	541, 1192
Bsu36I	CCTNAGG	1 cut(s)	708
BsuI	GTATCC	1 cut(s)	1152
BsuRI	GGCC	6 cut(s)	36, 192, 470, 587, 844, 962
BtsCI	GGATG	8 cut(s)	230, 307, 385, 1117, 1237, 1410, 1453, 1511
BtsI	GCAGTG	1 cut(s)	294
BtsIMutI	CAGTG	1 cut(s)	294
Cac8I	GCNNGC	5 cut(s)	185, 253, 478, 1084, 1117
CaiI	CAGNNNCTG	1 cut(s)	782
CfoI	GCGC	2 cut(s)	696, 1455
Cfr13I	GGNCC	5 cut(s)	468, 704, 835, 868, 961
CseI	GACGC	1 cut(s)	189
CsiI	ACCWGGT	1 cut(s)	631
Csp6I	GTAC	1 cut(s)	1494
CviAII	CATG	3 cut(s)	673, 1415, 1427
CviQI	GTAC	1 cut(s)	1494
DdeI	CTNAG	3 cut(s)	278, 708, 918
DpnI	GATC	6 cut(s)	543, 814, 897, 933, 1188, 1194
DpnII	GATC	6 cut(s)	541, 812, 895, 931, 1186, 1192
DraIII	CACNNNGTG	2 cut(s)	400, 980
DriI	GACNNNNNGTC	1 cut(s)	1063
EaeI	YGGCCR	1 cut(s)	190
Eam1104I	CTCTTC	1 cut(s)	1137
Eam1105I	GACNNNNNGTC	1 cut(s)	1063
EarI	CTCTTC	1 cut(s)	1137
Ecl136II	GAGCTC	2 cut(s)	1040, 1238
Eco24I	GRGCYC	2 cut(s)	1042, 1240
Eco47I	GGWCC	3 cut(s)	704, 835, 868
Eco53kI	GAGCTC	2 cut(s)	1040, 1238
Eco57I	CTGAAG	2 cut(s)	710, 1049
Eco81I	CCTNAGG	1 cut(s)	708
Eco88I	CYCGRG	1 cut(s)	447
Eco91I	GGTNACC	1 cut(s)	1132
EcoICRI	GAGCTC	2 cut(s)	1040, 1238
EcoO109I	RGGNCCY	3 cut(s)	835, 868, 961
EcoO65I	GGTNACC	1 cut(s)	1132
EcoRI	GAATTC	1 cut(s)	200
EcoRII	CCWGG	4 cut(s)	174, 631, 839, 1514
EcoT22I	ATGCAT	2 cut(s)	537, 1428
EcoT38I	GRGCYC	2 cut(s)	1042, 1240
FaeI	CATG	3 cut(s)	676, 1418, 1430
FalI	AAGNNNNNCTT	2 cut(s)	1357, 1389
FaqI	GGGAC	3 cut(s)	192, 848, 956
FatI	CATG	3 cut(s)	672, 1414, 1426
FbaI	TGATCA	1 cut(s)	931
FblI	GTMKAC	1 cut(s)	1434
Fnu4HI	GCNGC	5 cut(s)	329, 471, 474, 1215, 1476
FokI	GGATG	8 cut(s)	217, 314, 392, 1124, 1244, 1397, 1460, 1498
FriOI	GRGCYC	2 cut(s)	1042, 1240
Fsp4HI	GCNGC	5 cut(s)	329, 471, 474, 1215, 1476
FspBI	CTAG	2 cut(s)	846, 1043
GlaI	GCGC	2 cut(s)	695, 1454
GluI	GCNGC	5 cut(s)	329, 471, 474, 1215, 1476
GsaI	CCCAGC	2 cut(s)	732, 1172
GsuI	CTGGAG	2 cut(s)	663, 742
HaeIII	GGCC	6 cut(s)	36, 192, 470, 587, 844, 962
HgaI	GACGC	1 cut(s)	189
HhaI	GCGC	2 cut(s)	696, 1455
Hin1II	CATG	3 cut(s)	676, 1418, 1430
Hin6I	GCGC	2 cut(s)	694, 1453
HinP1I	GCGC	2 cut(s)	694, 1453
HincII	GTYRAC	3 cut(s)	715, 912, 1390
HindII	GTYRAC	3 cut(s)	715, 912, 1390
HindIII	AAGCTT	1 cut(s)	1549
HinfI	GANTC	3 cut(s)	391, 943, 1536
HpaI	GTTAAC	1 cut(s)	912
HphI	GGTGA	6 cut(s)	125, 434, 647, 992, 1265, 1412
Hpy166II	GTNNAC	6 cut(s)	222, 715, 912, 1273, 1390, 1435
Hpy188I	TCNGA	3 cut(s)	206, 690, 856
Hpy8I	GTNNAC	6 cut(s)	222, 715, 912, 1273, 1390, 1435
HpyAV	CCTTC	5 cut(s)	358, 571, 952, 1480, 1562
HpyCH4III	ACNGT	4 cut(s)	126, 988, 1270, 1581
HpyCH4IV	ACGT	1 cut(s)	1431
HpyF10VI	GCNNNNNNNGC	5 cut(s)	33, 189, 334, 959, 1125
HpyF3I	CTNAG	3 cut(s)	278, 708, 918
HpySE526I	ACGT	1 cut(s)	1431
Hsp92II	CATG	3 cut(s)	676, 1418, 1430
HspAI	GCGC	2 cut(s)	694, 1453
KflI	GGGWCCC	1 cut(s)	835
Ksp22I	TGATCA	1 cut(s)	931
KspAI	GTTAAC	1 cut(s)	912
Kzo9I	GATC	6 cut(s)	541, 812, 895, 931, 1186, 1192
LguI	GCTCTTC	1 cut(s)	1137
LmnI	GCTCC	2 cut(s)	682, 1235
Lsp1109I	GCAGC	2 cut(s)	460, 1462
LweI	GCATC	7 cut(s)	239, 376, 544, 1102, 1309, 1464, 1520
MabI	ACCWGGT	1 cut(s)	631
MaeI	CTAG	2 cut(s)	846, 1043
MaeII	ACGT	1 cut(s)	1431
MaeIII	GTNAC	7 cut(s)	16, 208, 355, 1132, 1264, 1343, 1460
MalI	GATC	6 cut(s)	543, 814, 897, 933, 1188, 1194
MboI	GATC	6 cut(s)	541, 812, 895, 931, 1186, 1192
MflI	RGATCY	2 cut(s)	541, 1192
MhlI	GDGCHC	3 cut(s)	224, 1042, 1240
MluCI	AATT	8 cut(s)	7, 200, 641, 804, 1007, 1353, 1361, 1419
Mph1103I	ATGCAT	2 cut(s)	537, 1428
MseI	TTAA	7 cut(s)	99, 546, 645, 911, 1278, 1364, 1376
MslI	CAYNNNNRTG	2 cut(s)	217, 401
MspA1I	CMGCKG	2 cut(s)	473, 1168
MspR9I	CCNGG	4 cut(s)	176, 633, 841, 1516
MvaI	CCWGG	4 cut(s)	176, 633, 841, 1516
MvnI	CGCG	1 cut(s)	183
MwoI	GCNNNNNNNGC	5 cut(s)	33, 189, 334, 959, 1125
NdeII	GATC	6 cut(s)	541, 812, 895, 931, 1186, 1192
NlaIII	CATG	3 cut(s)	676, 1418, 1430
NlaIV	GGNNCC	4 cut(s)	678, 836, 837, 963
NmeAIII	GCCGAG	1 cut(s)	218
NmuCI	GTSAC	1 cut(s)	355
NsiI	ATGCAT	2 cut(s)	537, 1428
PciSI	GCTCTTC	1 cut(s)	1137
PfeI	GAWTC	3 cut(s)	391, 943, 1536
PkrI	GCNGC	5 cut(s)	330, 472, 475, 1216, 1477
PpuMI	RGGWCCY	2 cut(s)	835, 868
PshBI	ATTAAT	1 cut(s)	99
Psp124BI	GAGCTC	2 cut(s)	1042, 1240
Psp5II	RGGWCCY	2 cut(s)	835, 868
Psp6I	CCWGG	4 cut(s)	174, 631, 839, 1514
PspEI	GGTNACC	1 cut(s)	1132
PspFI	CCCAGC	2 cut(s)	728, 1168
PspGI	CCWGG	4 cut(s)	174, 631, 839, 1514
PspN4I	GGNNCC	4 cut(s)	678, 836, 837, 963
PspPI	GGNCC	5 cut(s)	468, 704, 835, 868, 961
PspPPI	RGGWCCY	2 cut(s)	835, 868
PstI	CTGCAG	2 cut(s)	289, 478
PstNI	CAGNNNCTG	1 cut(s)	782
PsuI	RGATCY	2 cut(s)	541, 1192
PvuII	CAGCTG	1 cut(s)	1168
RsaI	GTAC	1 cut(s)	1495
RsaNI	GTAC	1 cut(s)	1494
RseI	CAYNNNNRTG	2 cut(s)	217, 401
SacI	GAGCTC	2 cut(s)	1042, 1240
SapI	GCTCTTC	1 cut(s)	1137
SaqAI	TTAA	7 cut(s)	99, 546, 645, 911, 1278, 1364, 1376
SatI	GCNGC	5 cut(s)	329, 471, 474, 1215, 1476
Sau3AI	GATC	6 cut(s)	541, 812, 895, 931, 1186, 1192
Sau96I	GGNCC	5 cut(s)	468, 704, 835, 868, 961
ScrFI	CCNGG	4 cut(s)	176, 633, 841, 1516
SduI	GDGCHC	3 cut(s)	224, 1042, 1240
SexAI	ACCWGGT	1 cut(s)	631
SfaNI	GCATC	7 cut(s)	239, 376, 544, 1102, 1309, 1464, 1520
SfcI	CTRYAG	3 cut(s)	144, 285, 474
SinI	GGWCC	3 cut(s)	704, 835, 868
SmiMI	CAYNNNNRTG	2 cut(s)	217, 401
Sse9I	AATT	8 cut(s)	7, 200, 641, 804, 1007, 1353, 1361, 1419
SsiI	CCGC	5 cut(s)	328, 436, 471, 907, 1214
SspI	AATATT	3 cut(s)	352, 1180, 1523
SspMI	CTAG	2 cut(s)	846, 1043
SstI	GAGCTC	2 cut(s)	1042, 1240
StyD4I	CCNGG	4 cut(s)	174, 631, 839, 1514
TaaI	ACNGT	4 cut(s)	126, 988, 1270, 1581
TaiI	ACGT	1 cut(s)	1434
TaqI	TCGA	7 cut(s)	151, 161, 266, 432, 811, 1224, 1326
TasI	AATT	8 cut(s)	7, 200, 641, 804, 1007, 1353, 1361, 1419
TauI	GCSGC	3 cut(s)	331, 473, 1217
TfiI	GAWTC	3 cut(s)	391, 943, 1536
Tru1I	TTAA	7 cut(s)	99, 546, 645, 911, 1278, 1364, 1376
Tru9I	TTAA	7 cut(s)	99, 546, 645, 911, 1278, 1364, 1376
TscAI	CASTG	1 cut(s)	294
TseFI	GTSAC	1 cut(s)	355
TseI	GCWGC	2 cut(s)	473, 1475
Tsp45I	GTSAC	1 cut(s)	355
TspDTI	ATGAA	3 cut(s)	1034, 1308, 1499
TspGWI	ACGGA	2 cut(s)	540, 805
TspRI	CASTG	1 cut(s)	294
VneI	GTGCAC	1 cut(s)	220
VpaK11BI	GGWCC	3 cut(s)	704, 835, 868
VspI	ATTAAT	1 cut(s)	99
XapI	RAATTY	1 cut(s)	200
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1337
XmiI	GTMKAC	1 cut(s)	1434
XspI	CTAG	2 cut(s)	846, 1043
Zsp2I	ATGCAT	2 cut(s)	537, 1428

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.