Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0008187

Physical Location & Seq

Genomic Coordinates Forward (+)

35923753 .. 35931339

Gene Structure 7587 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00028230.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1626 bp

ATGTGCATCTCCGGGCTTCAAAAGCCCTCAAGAATCGGCGCCCGAGGTGGCCGAATCGGGAGTTTTGACGGTGACCACGAAGTAGCGCCGCCACCCAACTTCTTTGCCTTGTTGCGCCTTCTACAGCCAAAATTGTGCAGCAAAGTTGCCACGGACTTGAAGAGGGAGAGAGAGACGAATCATGGGACGGGTGGCGCGGCCAACGATTTGTCATCAGGTGAGAAATTCGCCTGCAAATCGATTTCGAAGAAGAAGCTGAGGACTGCTGTGGATATTGAGGATGTGAGACGTGAGGTGGACATCATGAGGCATTTGCCTCAGCACCCCAACATTGTGTCATTGAAGGACACTTATGAAGACGACGAGGCGGTTCACATTGTGATGGAGTTGTGTGAGGGAGGCGAGCTGTTTGACCGCATTGTTGCTAGGGGTCACTATACGGAGCGTGCAGCTGCTGCTGTAATGCGGACAATTGTTGAAATTGTTCAGAGCTGTCACAAGCATGGAGTAATGCATCGCGATCTCAAACCAGAAAACTTTCTATTTGCAAATAAGAAAGAATCGGCTGCCTTAAAAGCAATTGATTTTGGGTTGTCCGTATTCTTTAAACCTGGTATTGCACTGCTTCCTTCTTGCATGTTTAATGATATTTCCGCAGATCTCAGAAGTGAGCAATTTAATGAGATAGTTGGAAGTCCGTACTACATGGCTCCAGAGGTTCTGAAGCGCAATTATGGTCCCGAGGTTGACATCTGGAGTGCCGGAGTAATCCTGTATATTTTACTTTGTGGTGTTCCACCTTTCTGGGCAGAAACTGAACAAGGAGTAGCACAAGCAATTATCCGCTCTATCATTGATTTCAAGAGGGACCCTTGGCCTAGAGTCTCTGATAATGCGAAGGACCTGGTAAAAAAAATGCTTGATCCTGATCCCAAGAAGCGGCTGACAGCTCAAGAAGTACTTGATCATCCTTGGATACAAAATGCCAAGAAGGCCCCAAATGTTCCACTTGGTGAGACTGTGAGAGCAAGGCTGAAACAATTTTCTGTAATGAACAAACTGAAAAAAAGAGCTCTGGGGGTCATAGCTGAGCATTTGTCAGTTGAGGAAGTAGCTGGCATAAAGGAGGCATTTCAACTGATGGATACTGATAAAAAAGGCAAGGTATCCCTGGATCAGCTTCGAAATGGAATACAAAAACTCGGCCAGCAGATTCCTGACCCAGATTTACAAATACTAATGGAAGCTGCTGATGTTGATGGCGATGGAGCTCTGAATTATGGAGAGTTTGTTGCGGTTTCGGTTCACCTTAAAAAGATGGCCAATGATGAGCACCTACATAAAGCTTTTGCATTCTTCGATCAAAACCAAAGTGGTTACATAGAAATTGAAGAGTTGCGAAATGCTTTAAATGATGATGTTGATGCAGGCGGCGAGGAAGTTATCCATGCAATTATGCATGATGTGGATACAGATAAGGATGGGCGCATAAGTTATGAGGAGTTTGTCGCTATGATGAAGTCTGGTACAGATTGGAGAAAAGCATCCAGACAATATTCACGGGAAAGATTCAACAGCATAAGCTTGAAGTTGATGCGTGAAGGGTCTTTTACAGTTAACCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8 Pkinase

Protein Analysis

541

Amino Acids

60.63

Weight (kDa)

6.19

Isoelectric Point (pI)

39.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	69 - 326	1.2e-69	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	73 - 321	1.6e-19	Protein tyrosine and serine/threonine kinase
EF-hand_7	PF13499	374 - 432	2e-10	EF-hand domain pair
EF-hand_6	PF13405	374 - 402	7.1e-06	EF-hand domain
EF-hand_7	PF13499	444 - 507	1.2e-15	EF-hand domain pair
EF-hand_6	PF13405	446 - 471	9.1e-06	EF-hand domain
EF-hand_1	PF00036	482 - 508	1.9e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0008187)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12480 AT5G12480 AT5G19450 AT5G19450
fragaria_vesca	FvH4_3g28740 FvH4_3g28740 FvH4_3g28740 FvH4_3g28740
malus_domestica	MD03G1173900.v1.1 MD11G1192100.v1.1
prunus_persica	Prupe.4G250200_v2.0.a1 Prupe.4G250200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0052321
rosa_laevigata	RLG00000034825
rosa_multiflora	Rmu_sc0000968.1_g000007 Rmu_sc0028377.1_g000006
rosa_roxburghii	Rroxscaffold_1G00028230
rosa_rugosa	Rorug05G0272800 Rorug05G0272900
rosa_samantha	Rh5AG345400 Rh5CG381800 Rh5DG371600
rosa_wichuraiana	Rw5G032600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	38
AccBSI	CCGCTC	1 cut(s)	846
AccII	CGCG	2 cut(s)	197, 519
AclWI	GGATC	3 cut(s)	917, 923, 1182
AcoI	YGGCCR	4 cut(s)	49, 198, 1204, 1320
AcsI	RAATTY	1 cut(s)	224
AcuI	CTGAAG	1 cut(s)	743
AcyI	GRCGYC	1 cut(s)	39
AdeI	CACNNNGTG	2 cut(s)	379, 1013
AfaI	GTAC	3 cut(s)	701, 960, 1528
AfiI	CCNNNNNNNGG	1 cut(s)	939
AgsI	TTSAA	9 cut(s)	20, 160, 343, 479, 862, 1136, 1391, 1573, 1588
AjiI	CACGTC	1 cut(s)	290
AjnI	CCWGG	3 cut(s)	610, 903, 1170
Alw21I	GWGCWC	3 cut(s)	1075, 1273, 1335
Alw26I	GTCTC	4 cut(s)	167, 280, 889, 1010
AlwI	GGATC	3 cut(s)	917, 923, 1182
AlwNI	CAGNNNCTG	2 cut(s)	455, 815
Ama87I	CYCGRG	2 cut(s)	42, 740
AoxI	GGCC	6 cut(s)	49, 198, 875, 993, 1204, 1320
ApeKI	GCWGC	6 cut(s)	138, 449, 452, 455, 566, 1247
ApoI	RAATTY	1 cut(s)	224
Asp700I	GAANNNNTTC	2 cut(s)	483, 537
AspLEI	GCGC	6 cut(s)	41, 88, 117, 197, 729, 1488
AspS9I	GGNCC	4 cut(s)	737, 868, 901, 994
AsuC2I	CCSGG	1 cut(s)	13
AsuHPI	GGTGA	4 cut(s)	83, 230, 1025, 1298
AsuII	TTCGAA	2 cut(s)	245, 1183
AvaI	CYCGRG	2 cut(s)	42, 740
AvaII	GGWCC	3 cut(s)	737, 868, 901
BalI	TGGCCA	1 cut(s)	1322
BanI	GGYRCC	1 cut(s)	38
BanII	GRGCYC	2 cut(s)	1075, 1273
BbsI	GAAGAC	1 cut(s)	363
Bbv12I	GWGCWC	3 cut(s)	1075, 1273, 1335
BbvCI	CCTCAGC	2 cut(s)	257, 318
BbvI	GCAGC	6 cut(s)	150, 439, 442, 461, 553, 1234
BccI	CCATC	6 cut(s)	376, 1135, 1253, 1259, 1312, 1475
BciT130I	CCWGG	3 cut(s)	612, 905, 1172
BciVI	GTATCC	4 cut(s)	969, 1138, 1177, 1462
BclI	TGATCA	1 cut(s)	964
BcnI	CCSGG	1 cut(s)	13
BcoDI	GTCTC	4 cut(s)	167, 280, 889, 1010
BfaI	CTAG	2 cut(s)	426, 879
BfmI	CTRYAG	1 cut(s)	122
BfoI	RGCGCY	2 cut(s)	42, 89
BfuI	GTATCC	4 cut(s)	969, 1138, 1177, 1462
BglI	GCCNNNNNGGC	1 cut(s)	992
BglII	AGATCT	1 cut(s)	658
BlpI	GCTNAGC	1 cut(s)	1089
BmcAI	AGTACT	1 cut(s)	960
Bme1390I	CCNGG	4 cut(s)	13, 612, 905, 1172
Bme18I	GGWCC	3 cut(s)	737, 868, 901
BmeT110I	CYCGRG	2 cut(s)	42, 740
BmgBI	CACGTC	1 cut(s)	290
BmgT120I	GGNCC	4 cut(s)	737, 868, 901, 994
BmiI	GGNNCC	6 cut(s)	40, 711, 739, 869, 870, 996
BmrFI	CCNGG	4 cut(s)	13, 612, 905, 1172
BmsI	GCATC	5 cut(s)	15, 523, 1414, 1553, 1584
BpiI	GAAGAC	1 cut(s)	363
BpmI	CTGGAG	2 cut(s)	696, 775
Bpu10I	CCTNAGC	2 cut(s)	257, 318
Bpu1102I	GCTNAGC	1 cut(s)	1089
Bpu14I	TTCGAA	2 cut(s)	245, 1183
BpuEI	CTTGAG	2 cut(s)	13, 936
BpuMI	CCSGG	1 cut(s)	13
Bsa29I	ATCGAT	1 cut(s)	239
BsaBI	GATNNNNATC	1 cut(s)	927
BsaHI	GRCGYC	1 cut(s)	39
BsaJI	CCNNGG	6 cut(s)	43, 150, 741, 872, 971, 1170
BsaXI	ACNNNNNCTCC	2 cut(s)	1275, 1305
Bsc4I	CCNNNNNNNGG	1 cut(s)	939
Bse8I	GATNNNNATC	1 cut(s)	927
BseBI	CCWGG	3 cut(s)	612, 905, 1172
BseCI	ATCGAT	1 cut(s)	239
BseDI	CCNNGG	6 cut(s)	43, 150, 741, 872, 971, 1170
BseGI	GGATG	4 cut(s)	286, 967, 1486, 1544
BseJI	GATNNNNATC	1 cut(s)	927
BseLI	CCNNNNNNNGG	1 cut(s)	939
BseMII	CTCAG	4 cut(s)	248, 332, 676, 1080
BseRI	GAGGAG	1 cut(s)	1514
BseXI	GCAGC	6 cut(s)	150, 439, 442, 461, 553, 1234
BsgI	GTGCAG	2 cut(s)	157, 468
Bsh1236I	CGCG	2 cut(s)	197, 519
BshFI	GGCC	6 cut(s)	51, 200, 877, 995, 1206, 1322
BshNI	GGYRCC	1 cut(s)	38
BshVI	ATCGAT	1 cut(s)	239
BsiHKAI	GWGCWC	3 cut(s)	1075, 1273, 1335
BsiHKCI	CYCGRG	2 cut(s)	42, 740
BsiSI	CCGG	2 cut(s)	12, 762
BslFI	GGGAC	3 cut(s)	199, 723, 881
BslI	CCNNNNNNNGG	1 cut(s)	939
BsmAI	GTCTC	4 cut(s)	167, 280, 889, 1010
BsmBI	CGTCTC	2 cut(s)	167, 280
BsmFI	GGGAC	3 cut(s)	199, 723, 881
BsmI	GAATGC	1 cut(s)	1352
BsnI	GGCC	6 cut(s)	51, 200, 877, 995, 1206, 1322
BsoBI	CYCGRG	2 cut(s)	42, 740
Bsp119I	TTCGAA	2 cut(s)	245, 1183
Bsp1286I	GDGCHC	3 cut(s)	1075, 1273, 1335
Bsp143I	GATC	7 cut(s)	520, 658, 922, 928, 964, 1174, 1360
Bsp1720I	GCTNAGC	1 cut(s)	1089
Bsp68I	TCGCGA	1 cut(s)	519
BspANI	GGCC	6 cut(s)	51, 200, 877, 995, 1206, 1322
BspCNI	CTCAG	4 cut(s)	249, 331, 675, 1081
BspDI	ATCGAT	1 cut(s)	239
BspFNI	CGCG	2 cut(s)	197, 519
BspHI	TCATGA	1 cut(s)	303
BspLI	GGNNCC	6 cut(s)	40, 711, 739, 869, 870, 996
BspPI	GGATC	3 cut(s)	917, 923, 1182
BspT104I	TTCGAA	2 cut(s)	245, 1183
BspT107I	GGYRCC	1 cut(s)	38
BsrBI	CCGCTC	1 cut(s)	846
BssECI	CCNNGG	6 cut(s)	43, 150, 741, 872, 971, 1170
BssMI	GATC	7 cut(s)	520, 658, 922, 928, 964, 1174, 1360
BssNI	GRCGYC	1 cut(s)	39
BssT1I	CCWWGG	2 cut(s)	872, 971
Bst2UI	CCWGG	3 cut(s)	612, 905, 1172
Bst4CI	ACNGT	3 cut(s)	71, 1021, 1615
Bst6I	CTCTTC	2 cut(s)	155, 1386
BstACI	GRCGYC	1 cut(s)	39
BstAPI	GCANNNNNTGC	1 cut(s)	455
BstBI	TTCGAA	2 cut(s)	245, 1183
BstC8I	GCNNGC	6 cut(s)	232, 404, 447, 1117, 1208, 1429
BstDEI	CTNAG	4 cut(s)	257, 318, 662, 1089
BstDSI	CCRYGG	1 cut(s)	150
BstEII	GGTNACC	1 cut(s)	71
BstF5I	GGATG	4 cut(s)	286, 967, 1486, 1544
BstFNI	CGCG	2 cut(s)	197, 519
BstH2I	RGCGCY	2 cut(s)	42, 89
BstHHI	GCGC	6 cut(s)	41, 88, 117, 197, 729, 1488
BstKTI	GATC	7 cut(s)	523, 661, 925, 931, 967, 1177, 1363
BstMAI	GTCTC	4 cut(s)	167, 280, 889, 1010
BstMBI	GATC	7 cut(s)	520, 658, 922, 928, 964, 1174, 1360
BstMWI	GCNNNNNNNGC	4 cut(s)	22, 455, 575, 992
BstNI	CCWGG	3 cut(s)	612, 905, 1172
BstNSI	RCATGY	1 cut(s)	640
BstPI	GGTNACC	1 cut(s)	71
BstSCI	CCNGG	4 cut(s)	11, 610, 903, 1170
BstSFI	CTRYAG	1 cut(s)	122
BstUI	CGCG	2 cut(s)	197, 519
BstV1I	GCAGC	6 cut(s)	150, 439, 442, 461, 553, 1234
BstV2I	GAAGAC	1 cut(s)	363
BstX2I	RGATCY	1 cut(s)	658
BstXI	CCANNNNNNTGG	1 cut(s)	804
BstYI	RGATCY	1 cut(s)	658
Bsu15I	ATCGAT	1 cut(s)	239
BsuI	GTATCC	4 cut(s)	969, 1138, 1177, 1462
BsuRI	GGCC	6 cut(s)	51, 200, 877, 995, 1206, 1322
BsuTUI	ATCGAT	1 cut(s)	239
BtgI	CCRYGG	1 cut(s)	150
BtgZI	GCGATG	2 cut(s)	500, 1278
BtrI	CACGTC	1 cut(s)	290
BtsCI	GGATG	4 cut(s)	286, 967, 1486, 1544
BtsI	GCAGTG	1 cut(s)	620
BtsIMutI	CAGTG	1 cut(s)	620
BtuMI	TCGCGA	1 cut(s)	519
Cac8I	GCNNGC	6 cut(s)	232, 404, 447, 1117, 1208, 1429
CaiI	CAGNNNCTG	2 cut(s)	455, 815
CciI	TCATGA	1 cut(s)	303
CfoI	GCGC	6 cut(s)	41, 88, 117, 197, 729, 1488
Cfr13I	GGNCC	4 cut(s)	737, 868, 901, 994
ClaI	ATCGAT	1 cut(s)	239
CsiI	ACCWGGT	2 cut(s)	610, 903
Csp6I	GTAC	3 cut(s)	700, 959, 1527
CviAII	CATG	7 cut(s)	182, 304, 503, 637, 706, 1448, 1460
CviQI	GTAC	3 cut(s)	700, 959, 1527
DdeI	CTNAG	4 cut(s)	257, 318, 662, 1089
DinI	GGCGCC	1 cut(s)	40
DpnI	GATC	7 cut(s)	522, 660, 924, 930, 966, 1176, 1362
DpnII	GATC	7 cut(s)	520, 658, 922, 928, 964, 1174, 1360
DraI	TTTAAA	2 cut(s)	607, 1410
DraIII	CACNNNGTG	2 cut(s)	379, 1013
EaeI	YGGCCR	4 cut(s)	49, 198, 1204, 1320
Eam1104I	CTCTTC	2 cut(s)	155, 1386
EarI	CTCTTC	2 cut(s)	155, 1386
Ecl136II	GAGCTC	2 cut(s)	1073, 1271
Eco130I	CCWWGG	2 cut(s)	872, 971
Eco24I	GRGCYC	2 cut(s)	1075, 1273
Eco47I	GGWCC	3 cut(s)	737, 868, 901
Eco53kI	GAGCTC	2 cut(s)	1073, 1271
Eco57I	CTGAAG	1 cut(s)	743
Eco88I	CYCGRG	2 cut(s)	42, 740
Eco91I	GGTNACC	1 cut(s)	71
EcoICRI	GAGCTC	2 cut(s)	1073, 1271
EcoO109I	RGGNCCY	3 cut(s)	868, 901, 994
EcoO65I	GGTNACC	1 cut(s)	71
EcoRII	CCWGG	3 cut(s)	610, 903, 1170
EcoT14I	CCWWGG	2 cut(s)	872, 971
EcoT22I	ATGCAT	2 cut(s)	516, 1461
EcoT38I	GRGCYC	2 cut(s)	1075, 1273
EgeI	GGCGCC	1 cut(s)	40
EheI	GGCGCC	1 cut(s)	40
ErhI	CCWWGG	2 cut(s)	872, 971
Esp3I	CGTCTC	2 cut(s)	167, 280
FaeI	CATG	7 cut(s)	185, 307, 506, 640, 709, 1451, 1463
FalI	AAGNNNNNCTT	2 cut(s)	945, 977
FaqI	GGGAC	3 cut(s)	199, 723, 881
FatI	CATG	7 cut(s)	181, 303, 502, 636, 705, 1447, 1459
FbaI	TGATCA	1 cut(s)	964
FokI	GGATG	4 cut(s)	293, 954, 1493, 1531
FriOI	GRGCYC	2 cut(s)	1075, 1273
FspBI	CTAG	2 cut(s)	426, 879
GlaI	GCGC	6 cut(s)	40, 87, 116, 196, 728, 1487
GsuI	CTGGAG	2 cut(s)	696, 775
HaeII	RGCGCY	2 cut(s)	42, 89
HaeIII	GGCC	6 cut(s)	51, 200, 877, 995, 1206, 1322
HapII	CCGG	2 cut(s)	12, 762
HhaI	GCGC	6 cut(s)	41, 88, 117, 197, 729, 1488
Hin1I	GRCGYC	1 cut(s)	39
Hin1II	CATG	7 cut(s)	185, 307, 506, 640, 709, 1451, 1463
Hin6I	GCGC	6 cut(s)	39, 86, 115, 195, 727, 1486
HinP1I	GCGC	6 cut(s)	39, 86, 115, 195, 727, 1486
HincII	GTYRAC	2 cut(s)	748, 1618
HindII	GTYRAC	2 cut(s)	748, 1618
HindIII	AAGCTT	2 cut(s)	1344, 1582
HinfI	GANTC	7 cut(s)	33, 54, 178, 560, 882, 1213, 1569
HpaI	GTTAAC	1 cut(s)	1618
HpaII	CCGG	2 cut(s)	12, 762
HphI	GGTGA	4 cut(s)	83, 230, 1025, 1298
Hpy166II	GTNNAC	5 cut(s)	298, 373, 748, 1306, 1618
Hpy188I	TCNGA	5 cut(s)	489, 665, 723, 889, 1275
Hpy8I	GTNNAC	5 cut(s)	298, 373, 748, 1306, 1618
Hpy99I	CGWCG	1 cut(s)	365
HpyAV	CCTTC	6 cut(s)	128, 337, 639, 892, 985, 1595
HpyCH4III	ACNGT	3 cut(s)	71, 1021, 1615
HpyCH4IV	ACGT	1 cut(s)	289
HpyF10VI	GCNNNNNNNGC	4 cut(s)	22, 455, 575, 992
HpyF3I	CTNAG	4 cut(s)	257, 318, 662, 1089
HpySE526I	ACGT	1 cut(s)	289
Hsp92I	GRCGYC	1 cut(s)	39
Hsp92II	CATG	7 cut(s)	185, 307, 506, 640, 709, 1451, 1463
HspAI	GCGC	6 cut(s)	39, 86, 115, 195, 727, 1486
KasI	GGCGCC	1 cut(s)	38
KflI	GGGWCCC	1 cut(s)	868
Ksp22I	TGATCA	1 cut(s)	964
KspAI	GTTAAC	1 cut(s)	1618
Kzo9I	GATC	7 cut(s)	520, 658, 922, 928, 964, 1174, 1360
LmnI	GCTCC	3 cut(s)	442, 715, 1268
Lsp1109I	GCAGC	6 cut(s)	150, 439, 442, 461, 553, 1234
LweI	GCATC	5 cut(s)	15, 523, 1414, 1553, 1584
MabI	ACCWGGT	2 cut(s)	610, 903
MaeI	CTAG	2 cut(s)	426, 879
MaeII	ACGT	1 cut(s)	289
MaeIII	GTNAC	4 cut(s)	71, 431, 494, 1376
MalI	GATC	7 cut(s)	522, 660, 924, 930, 966, 1176, 1362
MbiI	CCGCTC	1 cut(s)	846
MboI	GATC	7 cut(s)	520, 658, 922, 928, 964, 1174, 1360
MboII	GAAGA	6 cut(s)	172, 259, 262, 368, 1348, 1403
MfeI	CAATTG	2 cut(s)	471, 579
MflI	RGATCY	1 cut(s)	658
MhlI	GDGCHC	3 cut(s)	1075, 1273, 1335
MlsI	TGGCCA	1 cut(s)	1322
MluNI	TGGCCA	1 cut(s)	1322
Mly113I	GGCGCC	1 cut(s)	39
MlyI	GAGTC	1 cut(s)	891
MmeI	TCCRAC	1 cut(s)	670
Mox20I	TGGCCA	1 cut(s)	1322
Mph1103I	ATGCAT	2 cut(s)	516, 1461
MroXI	GAANNNNTTC	2 cut(s)	483, 537
MscI	TGGCCA	1 cut(s)	1322
MseI	TTAA	7 cut(s)	572, 606, 642, 678, 1311, 1409, 1617
MslI	CAYNNNNRTG	2 cut(s)	380, 501
Msp20I	TGGCCA	1 cut(s)	1322
MspA1I	CMGCKG	1 cut(s)	452
MspI	CCGG	2 cut(s)	12, 762
MspR9I	CCNGG	4 cut(s)	13, 612, 905, 1172
MunI	CAATTG	2 cut(s)	471, 579
Mva1269I	GAATGC	1 cut(s)	1352
MvaI	CCWGG	3 cut(s)	612, 905, 1172
MvnI	CGCG	2 cut(s)	197, 519
MwoI	GCNNNNNNNGC	4 cut(s)	22, 455, 575, 992
NarI	GGCGCC	1 cut(s)	39
NciI	CCSGG	1 cut(s)	13
NdeII	GATC	7 cut(s)	520, 658, 922, 928, 964, 1174, 1360
NlaIII	CATG	7 cut(s)	185, 307, 506, 640, 709, 1451, 1463
NlaIV	GGNNCC	6 cut(s)	40, 711, 739, 869, 870, 996
NmeAIII	GCCGAG	1 cut(s)	1182
NmuCI	GTSAC	3 cut(s)	71, 431, 494
NruI	TCGCGA	1 cut(s)	519
NsiI	ATGCAT	2 cut(s)	516, 1461
NspI	RCATGY	1 cut(s)	640
NspV	TTCGAA	2 cut(s)	245, 1183
PagI	TCATGA	1 cut(s)	303
PcsI	WCGNNNNNNNCGW	1 cut(s)	75
PctI	GAATGC	1 cut(s)	1352
PdmI	GAANNNNTTC	2 cut(s)	483, 537
PfeI	GAWTC	6 cut(s)	33, 54, 178, 560, 1213, 1569
PleI	GAGTC	1 cut(s)	890
PluTI	GGCGCC	1 cut(s)	42
PpsI	GAGTC	1 cut(s)	890
PpuMI	RGGWCCY	2 cut(s)	868, 901
Psp124BI	GAGCTC	2 cut(s)	1075, 1273
Psp5II	RGGWCCY	2 cut(s)	868, 901
Psp6I	CCWGG	3 cut(s)	610, 903, 1170
PspEI	GGTNACC	1 cut(s)	71
PspGI	CCWGG	3 cut(s)	610, 903, 1170
PspN4I	GGNNCC	6 cut(s)	40, 711, 739, 869, 870, 996
PspPI	GGNCC	4 cut(s)	737, 868, 901, 994
PspPPI	RGGWCCY	2 cut(s)	868, 901
PstNI	CAGNNNCTG	2 cut(s)	455, 815
PsuI	RGATCY	1 cut(s)	658
PvuII	CAGCTG	1 cut(s)	452
RruI	TCGCGA	1 cut(s)	519
RsaI	GTAC	3 cut(s)	701, 960, 1528
RsaNI	GTAC	3 cut(s)	700, 959, 1527
RseI	CAYNNNNRTG	2 cut(s)	380, 501
SacI	GAGCTC	2 cut(s)	1075, 1273
SaqAI	TTAA	7 cut(s)	572, 606, 642, 678, 1311, 1409, 1617
Sau3AI	GATC	7 cut(s)	520, 658, 922, 928, 964, 1174, 1360
Sau96I	GGNCC	4 cut(s)	737, 868, 901, 994
ScaI	AGTACT	1 cut(s)	960
SchI	GAGTC	1 cut(s)	891
ScrFI	CCNGG	4 cut(s)	13, 612, 905, 1172
SduI	GDGCHC	3 cut(s)	1075, 1273, 1335
SexAI	ACCWGGT	2 cut(s)	610, 903
SfaNI	GCATC	5 cut(s)	15, 523, 1414, 1553, 1584
SfcI	CTRYAG	1 cut(s)	122
SfoI	GGCGCC	1 cut(s)	40
SfuI	TTCGAA	2 cut(s)	245, 1183
SinI	GGWCC	3 cut(s)	737, 868, 901
SmiMI	CAYNNNNRTG	2 cut(s)	380, 501
SmlI	CTYRAG	2 cut(s)	28, 951
SmoI	CTYRAG	2 cut(s)	28, 951
SspDI	GGCGCC	1 cut(s)	38
SspI	AATATT	1 cut(s)	1556
SspMI	CTAG	2 cut(s)	426, 879
SstI	GAGCTC	2 cut(s)	1075, 1273
StyD4I	CCNGG	4 cut(s)	11, 610, 903, 1170
StyI	CCWWGG	2 cut(s)	872, 971
TaaI	ACNGT	3 cut(s)	71, 1021, 1615
TaiI	ACGT	1 cut(s)	292
TaqI	TCGA	4 cut(s)	239, 245, 1183, 1359
TatI	WGTACW	1 cut(s)	958
TauI	GCSGC	4 cut(s)	91, 200, 943, 1434
TfiI	GAWTC	6 cut(s)	33, 54, 178, 560, 1213, 1569
Tru1I	TTAA	7 cut(s)	572, 606, 642, 678, 1311, 1409, 1617
Tru9I	TTAA	7 cut(s)	572, 606, 642, 678, 1311, 1409, 1617
TscAI	CASTG	1 cut(s)	627
TseFI	GTSAC	3 cut(s)	71, 431, 494
TseI	GCWGC	6 cut(s)	138, 449, 452, 455, 566, 1247
Tsp45I	GTSAC	3 cut(s)	71, 431, 494
TspDTI	ATGAA	3 cut(s)	369, 1067, 1532
TspGWI	ACGGA	4 cut(s)	167, 455, 586, 687
TspRI	CASTG	1 cut(s)	627
VpaK11BI	GGWCC	3 cut(s)	737, 868, 901
XapI	RAATTY	1 cut(s)	224
XceI	RCATGY	1 cut(s)	640
XmnI	GAANNNNTTC	2 cut(s)	483, 537
XspI	CTAG	2 cut(s)	426, 879
ZrmI	AGTACT	1 cut(s)	960
Zsp2I	ATGCAT	2 cut(s)	516, 1461

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.