Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0008187

Physical Location & Seq

Genomic Coordinates Reverse (-)

49949582 .. 49954415

Gene Structure 4834 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG381800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1617 bp

ATGGGGAATTGCTGTGTAACCCTCGGTAACTCTGCCCAGAATAAGAAGAAGACAAAGAAGAAGCCAAATCCCTTTGCTACTGATTTTGGTGCTGCCAATGGCAATGAGGGTGGGGACAAGCTGTGTGTTTTGAAAGACCCGACAGGGAAAGACATTTCGGCCCAATATGATCTGGGTAGGGAGCTCGGCCGTGGAGAATTTGGAATCACTTACTTGTGCACAGATTTGTCATCAGGTGAGAAATTCGCCTGCAAATCGATTTCGAAGAAGAAGCTGAGGACTGCTGTGGATATTGAGGATGTGAGACGTGAGGTGGACATCATGAGGCATTTGCCTCAGCACCCCAACATTGTGTCATTGAAGGACACTTATGAAGACGAAGAGGCGGTTCACATTGTGATGGAGTTATGTGAGGGAGGCGAGCTGTTTGACCGCATCGTTGCTAGGGGTCACTATACGGAGCGTGCAGCTGCTGCTGTAATGCGGACAATTGTTGAAATTGTTCAGAGCTGTCACAAGCATGGAGTAATGCATCGCGATCTCAAACCAGAAAACTTTCTATTTGCAAATAAGAAAGAATCGGCTGCCTTAAAAGCAATTGATTTTGGGTTGTCCGTATTCTTTAAACCTGGTGAGCAATTTAATGAGATAGTCGGAAGTCCGTACTACATGGCTCCAGAGGTTCTGAAGCGCAATTATGGTCCCGAGGTTGACATCTGGAGTGCCGGAGTAATCCTGTATATTTTACTTTGTGGTGTTCCACCTTTCTGGGCAGAAACTGAACAAGGAGTAGCACAAGCAATTATCCGCTCTATCATTGATTTCAAGAGGGACCCTTGGCCTAGAGTCTCTGATAATGCGAAGGACCTGGTAAAAAAAATGCTTGATCCTGATCCCAAGAAGCGGCTGACAGCTCAAGAAGTACTTGATCATCCTTGGATACAAAATGCCAAGAAGGCCCCAAATGTTCCACTTGGTGAGACTGTGAGAGCAAGGCTGAAACAATTTTCTGTAATGAACAAACTAAAAAAGAGAGCTCTGGGGGTCATAGCCGAGCATTTGTCAGTTGAGGAAGTAGCTGGCATAAAGGAGGCATTTCAACTGATGGATACTGATAAAAAAGGCAAGGTATCCCTGGATCAGCTTCGAAATGGAATACAAAAACTCGGCCAGCAGATTCCTGACCCAGATTTACAAATACTAATGGAAGCTGCTGATGTTGATGGCGATGGAGCTCTGAATTATGGAGAGTTTGTTGCGGTTTCGGTTCACCTTAAAAAGATGGCCAATGATGAGCACCTACATAAAGCTTTTGCATTCTTCGATCAAAACCAAAGTGGTTACATAGAAATTGAAGAGTTGCGAAATGCTTTAAATGATGATGTTGATGCAGGTGGCGAGGAAGTTATCCATGCAATTATGCATGATGTGGATACAGATAAGGATGGGCGCATAAGTTATGAGGAGTTTGTCGCTATGATGAAGTCTGGTACAGATTGGAGAAAAGCATCCAGACAATATTCACGGGAAAGATTCAACAGCATAAGCTTGAAGTTGATGCGTGAAGGGTCTTTACAGTTAACCAATGAGGGCGCCGCCCCTCCAGGTGCTGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004683 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009931 GO:0009987 GO:0010033 GO:0010857 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0035556 GO:0036211 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0071944 GO:0097305 GO:0097306 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04626 ko05145 map04626 map05145

Pfam Domains

Protein Families

EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_7 EF-hand_8 Pkinase

Protein Analysis

538

Amino Acids

60.0

Weight (kDa)

6.03

Isoelectric Point (pI)

34.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	56 - 314	5e-80	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	58 - 309	6.8e-26	Protein tyrosine and serine/threonine kinase
EF-hand_7	PF13499	362 - 420	2e-10	EF-hand domain pair
EF-hand_6	PF13405	362 - 390	7e-06	EF-hand domain
EF-hand_7	PF13499	432 - 495	1.2e-15	EF-hand domain pair
EF-hand_6	PF13405	434 - 459	9.1e-06	EF-hand domain
EF-hand_1	PF00036	470 - 496	1.8e-07	EF hand domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0008187)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G12480 AT5G12480 AT5G19450 AT5G19450
fragaria_vesca	FvH4_3g28740 FvH4_3g28740 FvH4_3g28740 FvH4_3g28740
malus_domestica	MD03G1173900.v1.1 MD11G1192100.v1.1
prunus_persica	Prupe.4G250200_v2.0.a1 Prupe.4G250200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0052321
rosa_laevigata	RLG00000034825
rosa_multiflora	Rmu_sc0000968.1_g000007 Rmu_sc0028377.1_g000006
rosa_roxburghii	Rroxscaffold_1G00028230
rosa_rugosa	Rorug05G0272800 Rorug05G0272900
rosa_samantha	Rh5AG345400 Rh5CG381800 Rh5DG371600
rosa_wichuraiana	Rw5G032600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1382
Acc36I	ACCTGC	1 cut(s)	1382
AccB1I	GGYRCC	1 cut(s)	1592
AccBSI	CCGCTC	1 cut(s)	810
AccII	CGCG	1 cut(s)	537
AciI	CCGC	7 cut(s)	386, 433, 484, 808, 904, 1259, 1596
AclWI	GGATC	3 cut(s)	881, 887, 1146
AcoI	YGGCCR	3 cut(s)	187, 1168, 1284
AcsI	RAATTY	2 cut(s)	197, 242
AcuI	CTGAAG	1 cut(s)	707
AcyI	GRCGYC	1 cut(s)	1593
AdeI	CACNNNGTG	2 cut(s)	397, 977
AfaI	GTAC	3 cut(s)	665, 924, 1492
AfiI	CCNNNNNNNGG	1 cut(s)	903
AgsI	TTSAA	8 cut(s)	133, 361, 497, 826, 1100, 1355, 1537, 1552
AjiI	CACGTC	1 cut(s)	308
AjnI	CCWGG	4 cut(s)	628, 867, 1134, 1603
Alw21I	GWGCWC	5 cut(s)	186, 221, 1039, 1237, 1299
Alw26I	GTCTC	3 cut(s)	298, 853, 974
Alw44I	GTGCAC	1 cut(s)	217
AlwI	GGATC	3 cut(s)	881, 887, 1146
AlwNI	CAGNNNCTG	3 cut(s)	473, 779, 1610
Ama87I	CYCGRG	1 cut(s)	704
AoxI	GGCC	6 cut(s)	159, 187, 839, 957, 1168, 1284
ApaLI	GTGCAC	1 cut(s)	217
ApeKI	GCWGC	7 cut(s)	92, 467, 470, 473, 584, 1211, 1610
ApoI	RAATTY	2 cut(s)	197, 242
Asp700I	GAANNNNTTC	2 cut(s)	501, 555
AspLEI	GCGC	3 cut(s)	693, 1452, 1595
AspS9I	GGNCC	5 cut(s)	160, 701, 832, 865, 958
AsuHPI	GGTGA	4 cut(s)	248, 644, 989, 1262
AsuII	TTCGAA	2 cut(s)	263, 1147
AvaI	CYCGRG	1 cut(s)	704
AvaII	GGWCC	3 cut(s)	701, 832, 865
BaeGI	GKGCMC	1 cut(s)	221
BalI	TGGCCA	1 cut(s)	1286
BanI	GGYRCC	1 cut(s)	1592
BanII	GRGCYC	3 cut(s)	186, 1039, 1237
BarI	GAAGNNNNNNTAC	2 cut(s)	1557, 1589
BbsI	GAAGAC	2 cut(s)	56, 381
Bbv12I	GWGCWC	5 cut(s)	186, 221, 1039, 1237, 1299
BbvCI	CCTCAGC	2 cut(s)	275, 336
BbvI	GCAGC	7 cut(s)	79, 457, 460, 479, 571, 1198, 1597
BccI	CCATC	6 cut(s)	394, 1099, 1217, 1223, 1276, 1439
BceAI	ACGGC	1 cut(s)	174
BciT130I	CCWGG	4 cut(s)	630, 869, 1136, 1605
BciVI	GTATCC	4 cut(s)	933, 1102, 1141, 1426
BclI	TGATCA	1 cut(s)	928
BcoDI	GTCTC	3 cut(s)	298, 853, 974
BfaI	CTAG	2 cut(s)	444, 843
BfoI	RGCGCY	1 cut(s)	1596
BfuAI	ACCTGC	1 cut(s)	1382
BfuI	GTATCC	4 cut(s)	933, 1102, 1141, 1426
BglI	GCCNNNNNGGC	1 cut(s)	956
BisI	GCNGC	9 cut(s)	93, 468, 471, 474, 585, 905, 1212, 1596, 1611
BlsI	GCNGC	9 cut(s)	94, 469, 472, 475, 586, 906, 1213, 1597, 1612
BmcAI	AGTACT	1 cut(s)	924
Bme1390I	CCNGG	4 cut(s)	630, 869, 1136, 1605
Bme18I	GGWCC	3 cut(s)	701, 832, 865
BmeT110I	CYCGRG	1 cut(s)	704
BmgBI	CACGTC	1 cut(s)	308
BmgT120I	GGNCC	5 cut(s)	160, 701, 832, 865, 958
BmiI	GGNNCC	6 cut(s)	675, 703, 833, 834, 960, 1594
BmrFI	CCNGG	4 cut(s)	630, 869, 1136, 1605
BmsI	GCATC	5 cut(s)	444, 541, 1378, 1517, 1548
BpiI	GAAGAC	2 cut(s)	56, 381
BpmI	CTGGAG	3 cut(s)	660, 739, 1587
Bpu10I	CCTNAGC	2 cut(s)	275, 336
Bpu14I	TTCGAA	2 cut(s)	263, 1147
BpuEI	CTTGAG	1 cut(s)	900
Bsa29I	ATCGAT	1 cut(s)	257
BsaBI	GATNNNNATC	1 cut(s)	891
BsaHI	GRCGYC	1 cut(s)	1593
BsaJI	CCNNGG	6 cut(s)	22, 190, 705, 836, 935, 1134
BsaXI	ACNNNNNCTCC	2 cut(s)	1239, 1269
Bsc4I	CCNNNNNNNGG	1 cut(s)	903
Bse3DI	GCAATG	1 cut(s)	109
Bse8I	GATNNNNATC	1 cut(s)	891
BseBI	CCWGG	4 cut(s)	630, 869, 1136, 1605
BseCI	ATCGAT	1 cut(s)	257
BseDI	CCNNGG	6 cut(s)	22, 190, 705, 836, 935, 1134
BseGI	GGATG	4 cut(s)	304, 931, 1450, 1508
BseJI	GATNNNNATC	1 cut(s)	891
BseLI	CCNNNNNNNGG	1 cut(s)	903
BseMI	GCAATG	1 cut(s)	109
BseMII	CTCAG	2 cut(s)	266, 350
BseRI	GAGGAG	1 cut(s)	1478
BseSI	GKGCMC	1 cut(s)	221
BseX3I	CGGCCG	1 cut(s)	187
BseXI	GCAGC	7 cut(s)	79, 457, 460, 479, 571, 1198, 1597
BsgI	GTGCAG	1 cut(s)	486
Bsh1236I	CGCG	1 cut(s)	537
Bsh1285I	CGRYCG	1 cut(s)	190
BshFI	GGCC	6 cut(s)	161, 189, 841, 959, 1170, 1286
BshNI	GGYRCC	1 cut(s)	1592
BshVI	ATCGAT	1 cut(s)	257
BsiEI	CGRYCG	1 cut(s)	190
BsiHKAI	GWGCWC	5 cut(s)	186, 221, 1039, 1237, 1299
BsiHKCI	CYCGRG	1 cut(s)	704
BsiSI	CCGG	1 cut(s)	726
BslFI	GGGAC	3 cut(s)	128, 687, 845
BslI	CCNNNNNNNGG	1 cut(s)	903
BsmAI	GTCTC	3 cut(s)	298, 853, 974
BsmBI	CGTCTC	1 cut(s)	298
BsmFI	GGGAC	3 cut(s)	128, 687, 845
BsmI	GAATGC	1 cut(s)	1316
BsnI	GGCC	6 cut(s)	161, 189, 841, 959, 1170, 1286
BsoBI	CYCGRG	1 cut(s)	704
Bsp119I	TTCGAA	2 cut(s)	263, 1147
Bsp1286I	GDGCHC	5 cut(s)	186, 221, 1039, 1237, 1299
Bsp143I	GATC	7 cut(s)	169, 538, 886, 892, 928, 1138, 1324
Bsp68I	TCGCGA	1 cut(s)	537
BspACI	CCGC	7 cut(s)	386, 433, 484, 808, 904, 1259, 1596
BspANI	GGCC	6 cut(s)	161, 189, 841, 959, 1170, 1286
BspCNI	CTCAG	2 cut(s)	267, 349
BspDI	ATCGAT	1 cut(s)	257
BspFNI	CGCG	1 cut(s)	537
BspHI	TCATGA	1 cut(s)	321
BspLI	GGNNCC	6 cut(s)	675, 703, 833, 834, 960, 1594
BspMI	ACCTGC	1 cut(s)	1382
BspPI	GGATC	3 cut(s)	881, 887, 1146
BspT104I	TTCGAA	2 cut(s)	263, 1147
BspT107I	GGYRCC	1 cut(s)	1592
BsrBI	CCGCTC	1 cut(s)	810
BsrDI	GCAATG	1 cut(s)	109
BssECI	CCNNGG	6 cut(s)	22, 190, 705, 836, 935, 1134
BssMI	GATC	7 cut(s)	169, 538, 886, 892, 928, 1138, 1324
BssNI	GRCGYC	1 cut(s)	1593
BssT1I	CCWWGG	2 cut(s)	836, 935
Bst2UI	CCWGG	4 cut(s)	630, 869, 1136, 1605
Bst4CI	ACNGT	2 cut(s)	985, 1578
Bst6I	CTCTTC	2 cut(s)	375, 1350
BstACI	GRCGYC	1 cut(s)	1593
BstAPI	GCANNNNNTGC	1 cut(s)	473
BstBI	TTCGAA	2 cut(s)	263, 1147
BstC8I	GCNNGC	5 cut(s)	250, 422, 465, 1081, 1172
BstDEI	CTNAG	2 cut(s)	275, 336
BstDSI	CCRYGG	1 cut(s)	190
BstF5I	GGATG	4 cut(s)	304, 931, 1450, 1508
BstFNI	CGCG	1 cut(s)	537
BstH2I	RGCGCY	1 cut(s)	1596
BstHHI	GCGC	3 cut(s)	693, 1452, 1595
BstKTI	GATC	7 cut(s)	172, 541, 889, 895, 931, 1141, 1327
BstMAI	GTCTC	3 cut(s)	298, 853, 974
BstMBI	GATC	7 cut(s)	169, 538, 886, 892, 928, 1138, 1324
BstMCI	CGRYCG	1 cut(s)	190
BstMWI	GCNNNNNNNGC	3 cut(s)	473, 593, 956
BstNI	CCWGG	4 cut(s)	630, 869, 1136, 1605
BstSCI	CCNGG	4 cut(s)	628, 867, 1134, 1603
BstSLI	GKGCMC	1 cut(s)	221
BstUI	CGCG	1 cut(s)	537
BstV1I	GCAGC	7 cut(s)	79, 457, 460, 479, 571, 1198, 1597
BstV2I	GAAGAC	2 cut(s)	56, 381
BstXI	CCANNNNNNTGG	1 cut(s)	768
BstZI	CGGCCG	1 cut(s)	187
Bsu15I	ATCGAT	1 cut(s)	257
BsuI	GTATCC	4 cut(s)	933, 1102, 1141, 1426
BsuRI	GGCC	6 cut(s)	161, 189, 841, 959, 1170, 1286
BsuTUI	ATCGAT	1 cut(s)	257
BtgI	CCRYGG	1 cut(s)	190
BtgZI	GCGATG	2 cut(s)	518, 1242
BtrI	CACGTC	1 cut(s)	308
BtsCI	GGATG	4 cut(s)	304, 931, 1450, 1508
BtuMI	TCGCGA	1 cut(s)	537
BveI	ACCTGC	1 cut(s)	1382
Cac8I	GCNNGC	5 cut(s)	250, 422, 465, 1081, 1172
CaiI	CAGNNNCTG	3 cut(s)	473, 779, 1610
CciI	TCATGA	1 cut(s)	321
CfoI	GCGC	3 cut(s)	693, 1452, 1595
Cfr13I	GGNCC	5 cut(s)	160, 701, 832, 865, 958
ClaI	ATCGAT	1 cut(s)	257
CsiI	ACCWGGT	2 cut(s)	628, 867
Csp6I	GTAC	3 cut(s)	664, 923, 1491
CspCI	CAANNNNNGTGG	2 cut(s)	91, 126
CviAII	CATG	6 cut(s)	322, 521, 670, 1412, 1424, 1614
CviQI	GTAC	3 cut(s)	664, 923, 1491
DdeI	CTNAG	2 cut(s)	275, 336
DinI	GGCGCC	1 cut(s)	1594
DpnI	GATC	7 cut(s)	171, 540, 888, 894, 930, 1140, 1326
DpnII	GATC	7 cut(s)	169, 538, 886, 892, 928, 1138, 1324
DraI	TTTAAA	2 cut(s)	625, 1374
DraIII	CACNNNGTG	2 cut(s)	397, 977
EaeI	YGGCCR	3 cut(s)	187, 1168, 1284
EagI	CGGCCG	1 cut(s)	187
Eam1104I	CTCTTC	2 cut(s)	375, 1350
EarI	CTCTTC	2 cut(s)	375, 1350
Ecl136II	GAGCTC	3 cut(s)	184, 1037, 1235
EclXI	CGGCCG	1 cut(s)	187
Eco130I	CCWWGG	2 cut(s)	836, 935
Eco24I	GRGCYC	3 cut(s)	186, 1039, 1237
Eco47I	GGWCC	3 cut(s)	701, 832, 865
Eco52I	CGGCCG	1 cut(s)	187
Eco53kI	GAGCTC	3 cut(s)	184, 1037, 1235
Eco57I	CTGAAG	1 cut(s)	707
Eco88I	CYCGRG	1 cut(s)	704
EcoICRI	GAGCTC	3 cut(s)	184, 1037, 1235
EcoO109I	RGGNCCY	3 cut(s)	832, 865, 958
EcoRII	CCWGG	4 cut(s)	628, 867, 1134, 1603
EcoT14I	CCWWGG	2 cut(s)	836, 935
EcoT22I	ATGCAT	2 cut(s)	534, 1425
EcoT38I	GRGCYC	3 cut(s)	186, 1039, 1237
EgeI	GGCGCC	1 cut(s)	1594
EheI	GGCGCC	1 cut(s)	1594
ErhI	CCWWGG	2 cut(s)	836, 935
Esp3I	CGTCTC	1 cut(s)	298
FaeI	CATG	6 cut(s)	325, 524, 673, 1415, 1427, 1617
FalI	AAGNNNNNCTT	2 cut(s)	909, 941
FaqI	GGGAC	3 cut(s)	128, 687, 845
FatI	CATG	6 cut(s)	321, 520, 669, 1411, 1423, 1613
FbaI	TGATCA	1 cut(s)	928
Fnu4HI	GCNGC	9 cut(s)	93, 468, 471, 474, 585, 905, 1212, 1596, 1611
FokI	GGATG	4 cut(s)	311, 918, 1457, 1495
FriOI	GRGCYC	3 cut(s)	186, 1039, 1237
Fsp4HI	GCNGC	9 cut(s)	93, 468, 471, 474, 585, 905, 1212, 1596, 1611
FspBI	CTAG	2 cut(s)	444, 843
GlaI	GCGC	3 cut(s)	692, 1451, 1594
GluI	GCNGC	9 cut(s)	93, 468, 471, 474, 585, 905, 1212, 1596, 1611
GsuI	CTGGAG	3 cut(s)	660, 739, 1587
HaeII	RGCGCY	1 cut(s)	1596
HaeIII	GGCC	6 cut(s)	161, 189, 841, 959, 1170, 1286
HapII	CCGG	1 cut(s)	726
HhaI	GCGC	3 cut(s)	693, 1452, 1595
Hin1I	GRCGYC	1 cut(s)	1593
Hin1II	CATG	6 cut(s)	325, 524, 673, 1415, 1427, 1617
Hin6I	GCGC	3 cut(s)	691, 1450, 1593
HinP1I	GCGC	3 cut(s)	691, 1450, 1593
HincII	GTYRAC	2 cut(s)	712, 1581
HindII	GTYRAC	2 cut(s)	712, 1581
HindIII	AAGCTT	2 cut(s)	1308, 1546
HinfI	GANTC	5 cut(s)	204, 578, 846, 1177, 1533
HpaI	GTTAAC	1 cut(s)	1581
HpaII	CCGG	1 cut(s)	726
HphI	GGTGA	4 cut(s)	248, 644, 989, 1262
Hpy166II	GTNNAC	6 cut(s)	219, 316, 391, 712, 1270, 1581
Hpy188I	TCNGA	5 cut(s)	507, 656, 687, 853, 1239
Hpy8I	GTNNAC	6 cut(s)	219, 316, 391, 712, 1270, 1581
HpyAV	CCTTC	4 cut(s)	355, 856, 949, 1559
HpyCH4III	ACNGT	2 cut(s)	985, 1578
HpyCH4IV	ACGT	1 cut(s)	307
HpyF10VI	GCNNNNNNNGC	3 cut(s)	473, 593, 956
HpyF3I	CTNAG	2 cut(s)	275, 336
HpySE526I	ACGT	1 cut(s)	307
Hsp92I	GRCGYC	1 cut(s)	1593
Hsp92II	CATG	6 cut(s)	325, 524, 673, 1415, 1427, 1617
HspAI	GCGC	3 cut(s)	691, 1450, 1593
KasI	GGCGCC	1 cut(s)	1592
KflI	GGGWCCC	1 cut(s)	832
Ksp22I	TGATCA	1 cut(s)	928
KspAI	GTTAAC	1 cut(s)	1581
Kzo9I	GATC	7 cut(s)	169, 538, 886, 892, 928, 1138, 1324
LmnI	GCTCC	4 cut(s)	181, 460, 679, 1232
Lsp1109I	GCAGC	7 cut(s)	79, 457, 460, 479, 571, 1198, 1597
LweI	GCATC	5 cut(s)	444, 541, 1378, 1517, 1548
MabI	ACCWGGT	2 cut(s)	628, 867
MaeI	CTAG	2 cut(s)	444, 843
MaeII	ACGT	1 cut(s)	307
MaeIII	GTNAC	5 cut(s)	16, 26, 449, 512, 1340
MalI	GATC	7 cut(s)	171, 540, 888, 894, 930, 1140, 1326
MbiI	CCGCTC	1 cut(s)	810
MboI	GATC	7 cut(s)	169, 538, 886, 892, 928, 1138, 1324
MboII	GAAGA	9 cut(s)	58, 61, 70, 277, 280, 386, 392, 1312, 1367
MfeI	CAATTG	2 cut(s)	489, 597
MhlI	GDGCHC	5 cut(s)	186, 221, 1039, 1237, 1299
MlsI	TGGCCA	1 cut(s)	1286
MluNI	TGGCCA	1 cut(s)	1286
Mly113I	GGCGCC	1 cut(s)	1593
MlyI	GAGTC	1 cut(s)	855
MmeI	TCCRAC	1 cut(s)	634
Mox20I	TGGCCA	1 cut(s)	1286
Mph1103I	ATGCAT	2 cut(s)	534, 1425
MroXI	GAANNNNTTC	2 cut(s)	501, 555
MscI	TGGCCA	1 cut(s)	1286
MseI	TTAA	6 cut(s)	590, 624, 642, 1275, 1373, 1580
MslI	CAYNNNNRTG	2 cut(s)	398, 519
Msp20I	TGGCCA	1 cut(s)	1286
MspA1I	CMGCKG	1 cut(s)	470
MspI	CCGG	1 cut(s)	726
MspR9I	CCNGG	4 cut(s)	630, 869, 1136, 1605
MunI	CAATTG	2 cut(s)	489, 597
Mva1269I	GAATGC	1 cut(s)	1316
MvaI	CCWGG	4 cut(s)	630, 869, 1136, 1605
MvnI	CGCG	1 cut(s)	537
MwoI	GCNNNNNNNGC	3 cut(s)	473, 593, 956
NarI	GGCGCC	1 cut(s)	1593
NdeII	GATC	7 cut(s)	169, 538, 886, 892, 928, 1138, 1324
NlaIII	CATG	6 cut(s)	325, 524, 673, 1415, 1427, 1617
NlaIV	GGNNCC	6 cut(s)	675, 703, 833, 834, 960, 1594
NmeAIII	GCCGAG	3 cut(s)	165, 1078, 1146
NmuCI	GTSAC	2 cut(s)	449, 512
NruI	TCGCGA	1 cut(s)	537
NsiI	ATGCAT	2 cut(s)	534, 1425
NspV	TTCGAA	2 cut(s)	263, 1147
PagI	TCATGA	1 cut(s)	321
PaqCI	CACCTGC	1 cut(s)	1382
PctI	GAATGC	1 cut(s)	1316
PdmI	GAANNNNTTC	2 cut(s)	501, 555
PfeI	GAWTC	4 cut(s)	204, 578, 1177, 1533
PkrI	GCNGC	9 cut(s)	94, 469, 472, 475, 586, 906, 1213, 1597, 1612
PleI	GAGTC	1 cut(s)	854
PluTI	GGCGCC	1 cut(s)	1596
PpsI	GAGTC	1 cut(s)	854
PpuMI	RGGWCCY	2 cut(s)	832, 865
Psp124BI	GAGCTC	3 cut(s)	186, 1039, 1237
Psp5II	RGGWCCY	2 cut(s)	832, 865
Psp6I	CCWGG	4 cut(s)	628, 867, 1134, 1603
PspGI	CCWGG	4 cut(s)	628, 867, 1134, 1603
PspN4I	GGNNCC	6 cut(s)	675, 703, 833, 834, 960, 1594
PspPI	GGNCC	5 cut(s)	160, 701, 832, 865, 958
PspPPI	RGGWCCY	2 cut(s)	832, 865
PstNI	CAGNNNCTG	3 cut(s)	473, 779, 1610
PvuII	CAGCTG	1 cut(s)	470
RruI	TCGCGA	1 cut(s)	537
RsaI	GTAC	3 cut(s)	665, 924, 1492
RsaNI	GTAC	3 cut(s)	664, 923, 1491
RseI	CAYNNNNRTG	2 cut(s)	398, 519
SacI	GAGCTC	3 cut(s)	186, 1039, 1237
SaqAI	TTAA	6 cut(s)	590, 624, 642, 1275, 1373, 1580
SatI	GCNGC	9 cut(s)	93, 468, 471, 474, 585, 905, 1212, 1596, 1611
Sau3AI	GATC	7 cut(s)	169, 538, 886, 892, 928, 1138, 1324
Sau96I	GGNCC	5 cut(s)	160, 701, 832, 865, 958
ScaI	AGTACT	1 cut(s)	924
SchI	GAGTC	1 cut(s)	855
ScrFI	CCNGG	4 cut(s)	630, 869, 1136, 1605
SduI	GDGCHC	5 cut(s)	186, 221, 1039, 1237, 1299
SexAI	ACCWGGT	2 cut(s)	628, 867
SfaNI	GCATC	5 cut(s)	444, 541, 1378, 1517, 1548
SfoI	GGCGCC	1 cut(s)	1594
SfuI	TTCGAA	2 cut(s)	263, 1147
SinI	GGWCC	3 cut(s)	701, 832, 865
SmiMI	CAYNNNNRTG	2 cut(s)	398, 519
SmlI	CTYRAG	1 cut(s)	915
SmoI	CTYRAG	1 cut(s)	915
SsiI	CCGC	7 cut(s)	386, 433, 484, 808, 904, 1259, 1596
SspDI	GGCGCC	1 cut(s)	1592
SspI	AATATT	1 cut(s)	1520
SspMI	CTAG	2 cut(s)	444, 843
SstI	GAGCTC	3 cut(s)	186, 1039, 1237
StyD4I	CCNGG	4 cut(s)	628, 867, 1134, 1603
StyI	CCWWGG	2 cut(s)	836, 935
TaaI	ACNGT	2 cut(s)	985, 1578
TaiI	ACGT	1 cut(s)	310
TaqI	TCGA	4 cut(s)	257, 263, 1147, 1323
TatI	WGTACW	1 cut(s)	922
TauI	GCSGC	2 cut(s)	907, 1598
TfiI	GAWTC	4 cut(s)	204, 578, 1177, 1533
Tru1I	TTAA	6 cut(s)	590, 624, 642, 1275, 1373, 1580
Tru9I	TTAA	6 cut(s)	590, 624, 642, 1275, 1373, 1580
TseFI	GTSAC	2 cut(s)	449, 512
TseI	GCWGC	7 cut(s)	92, 467, 470, 473, 584, 1211, 1610
Tsp45I	GTSAC	2 cut(s)	449, 512
TspDTI	ATGAA	3 cut(s)	387, 1031, 1496
TspGWI	ACGGA	3 cut(s)	473, 604, 651
VneI	GTGCAC	1 cut(s)	217
VpaK11BI	GGWCC	3 cut(s)	701, 832, 865
XapI	RAATTY	2 cut(s)	197, 242
XmnI	GAANNNNTTC	2 cut(s)	501, 555
XspI	CTAG	2 cut(s)	444, 843
ZrmI	AGTACT	1 cut(s)	924
Zsp2I	ATGCAT	2 cut(s)	534, 1425

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.