Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0007213

Physical Location & Seq

Genomic Coordinates Reverse (-)

23867258 .. 23872067

Gene Structure 4810 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1174600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1077 bp

ATGCAGCCAAAATCTGAAGCTGAAAATCGACTACATGCTCATACTGTCCCACCAAGCAGTGTTTATTCGGAACCATGGTGGCAGAGTGTTGGATATAATCCCATCTCCCCAACTGGAACCGGGAGGAATGTATCCAACACATCTTCGTTAGAATGCCCAAATGGTCTGTCAGAGTCTAATGATGATCAATCTTTGTCCAATGATGAGGCAAATGAAGATGATGATGATACTGCCAAAGATTCACATACTACATCTCCAAGATCAGATAGCACTTCCTATTATATCTGGGAATTTGCTGGAAATTATGGGCAAGACCACCACAATGTAAAGCATGCTGGATCCAGTGTACCTACAGTGCGTGATGATTGCCTTGCGCAACCACCACAGCTTGAACTTGTTGGTCACTCTATTGCGTGTGCTTCAAATCCTTATCAGGATCCTTGTTATGGTGGAATGATGGCAGCTTATGGATATCCACCTTTGGGTTATCCTCCTTTTCTTGGAATGCCGCATGCTAGAATGCCATTGCCTCTTGAGATGGCACAGGAGCCTGTGTATGTAAATGCAAAACAATACCAAGGTATTCTCAGGCGAAGACAGGCACGTGCAAAAGCAGAACTTGAAAGGAAGCTAATAAAAGTCAGGAAGCCGTATCTTCATGAATCACGACATCAACATGCTATGAGAAGGGCGAGAGGTACTGGAGGACGTTTTGCAAAGAAAGCTAATGGTGATACTTCAAACAGCACTGGACAAGAAAAGGGTACAAGTTCTGGTCCAGCCCACTCATCACAATCTGGCAGTTCATCGGGTTCAGAACCCTTTCCGTCAGATTCTGCCGAAACATGGAATTCCCCCAATAGTCAACGGGAAGCAAGAGGTTCCCAAGCGCACAATGCGTATGCGGCTCACAATTATGCAAATGGTAGTGGCTGCTGCCAGCCCCATGGTGGCTTGCAGGCTTCAATATATCCTTCATACTCGGGCAAGAGAGGGGAAGAAGGAGATTGTACAGGCCAGCAACGGGGAAGCATATCTTCGAACCAGGCCTCACAGAGGCGTCTTGCCATCCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005667 GO:0006355 GO:0007275 GO:0008150 GO:0009555 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009890 GO:0009892 GO:0010154 GO:0010262 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0016602 GO:0019219 GO:0019222 GO:0022414 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032501 GO:0032502 GO:0032991 GO:0040034 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044798 GO:0045892 GO:0045934 GO:0048229 GO:0048316 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0055046 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090575 GO:0140110 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04612 ko05152 map04612 map05152

Pfam Domains

Protein Families

CBFB_NFYA

Protein Analysis

359

Amino Acids

38.85

Weight (kDa)

6.73

Isoelectric Point (pI)

57.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBFB_NFYA	PF02045	183 - 238	8.5e-27	CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007213)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G20910 AT5G12840 AT5G12840 AT5G12840 AT5G12840 AT5G12840
fragaria_vesca	FvH4_3g28780 FvH4_3g28780 FvH4_3g28780
malus_domestica	MD03G1174600.v1.1 MD11G1192400.v1.1
prunus_persica	Prupe.4G249900_v2.0.a1 Prupe.4G249900_v2.0.a1
pyrus_communis	pycom03g13200 pycom11g16820
rosa_chinensis	RchiOBHm_Chr5g0052231
rosa_laevigata	RLG00000034835
rosa_multiflora	Rmu_sc0003759.1_g000007
rosa_roxburghii	Rroxscaffold_1G00028070
rosa_rugosa	Rorug05G0273700
rosa_samantha	Rh5AG346500 Rh5BG357100 Rh5CG382500 Rh5DG372500
rosa_wichuraiana	Rw5G032720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	375
AciI	CCGC	2 cut(s)	509, 905
AclWI	GGATC	4 cut(s)	333, 346, 431, 444
AcsI	RAATTY	2 cut(s)	290, 850
AcuI	CTGAAG	1 cut(s)	36
AcvI	CACGTG	1 cut(s)	605
AcyI	GRCGYC	1 cut(s)	1060
AfaI	GTAC	4 cut(s)	348, 700, 766, 1012
AfiI	CCNNNNNNNGG	7 cut(s)	446, 482, 500, 846, 950, 1024, 1056
AgsI	TTSAA	5 cut(s)	392, 423, 623, 741, 966
AjnI	CCWGG	1 cut(s)	1044
AluBI	AGCT	5 cut(s)	20, 388, 464, 631, 725
AluI	AGCT	5 cut(s)	20, 388, 464, 631, 725
AlwI	GGATC	4 cut(s)	333, 346, 431, 444
AlwNI	CAGNNNCTG	1 cut(s)	836
Ama87I	CYCGRG	1 cut(s)	982
AoxI	GGCC	2 cut(s)	1015, 1047
ApeKI	GCWGC	4 cut(s)	4, 461, 933, 936
ApoI	RAATTY	2 cut(s)	290, 850
ArsI	GACNNNNNNTTYG	2 cut(s)	152, 184
Asp700I	GAANNNNTTC	1 cut(s)	822
AspLEI	GCGC	2 cut(s)	376, 892
AspS9I	GGNCC	1 cut(s)	776
AsuC2I	CCSGG	1 cut(s)	121
AsuHPI	GGTGA	1 cut(s)	743
AsuII	TTCGAA	1 cut(s)	1040
AvaI	CYCGRG	1 cut(s)	982
AvaII	GGWCC	1 cut(s)	776
BamHI	GGATCC	2 cut(s)	338, 436
BbrPI	CACGTG	1 cut(s)	605
BbsI	GAAGAC	1 cut(s)	601
BbvI	GCAGC	4 cut(s)	16, 473, 920, 923
BccI	CCATC	4 cut(s)	110, 451, 532, 1076
BceAI	ACGGC	1 cut(s)	634
BciT130I	CCWGG	1 cut(s)	1046
BciVI	GTATCC	1 cut(s)	142
BclI	TGATCA	1 cut(s)	184
BcnI	CCSGG	1 cut(s)	121
BfaI	CTAG	1 cut(s)	516
BfmI	CTRYAG	1 cut(s)	351
BfuI	GTATCC	1 cut(s)	142
BisI	GCNGC	6 cut(s)	5, 462, 509, 906, 934, 937
BlsI	GCNGC	6 cut(s)	6, 463, 510, 907, 935, 938
Bme1390I	CCNGG	2 cut(s)	121, 1046
Bme18I	GGWCC	1 cut(s)	776
BmeT110I	CYCGRG	1 cut(s)	982
BmgT120I	GGNCC	1 cut(s)	776
BmiI	GGNNCC	6 cut(s)	72, 118, 340, 438, 549, 883
BmrFI	CCNGG	2 cut(s)	121, 1046
BpiI	GAAGAC	1 cut(s)	601
BpmI	CTGGAG	1 cut(s)	723
Bpu14I	TTCGAA	1 cut(s)	1040
BpuEI	CTTGAG	1 cut(s)	554
BpuMI	CCSGG	1 cut(s)	121
BsaAI	YACGTR	1 cut(s)	605
BsaHI	GRCGYC	1 cut(s)	1060
BsaJI	CCNNGG	3 cut(s)	74, 577, 946
BsaXI	ACNNNNNCTCC	4 cut(s)	238, 268, 539, 569
Bsc4I	CCNNNNNNNGG	7 cut(s)	446, 482, 500, 846, 950, 1024, 1056
Bse1I	ACTGG	5 cut(s)	118, 342, 706, 754, 1072
Bse3DI	GCAATG	1 cut(s)	524
BseBI	CCWGG	1 cut(s)	1046
BseDI	CCNNGG	3 cut(s)	74, 577, 946
BseGI	GGATG	1 cut(s)	1068
BseLI	CCNNNNNNNGG	7 cut(s)	446, 482, 500, 846, 950, 1024, 1056
BseMI	GCAATG	1 cut(s)	524
BseMII	CTCAG	1 cut(s)	601
BseNI	ACTGG	5 cut(s)	118, 342, 706, 754, 1072
BseXI	GCAGC	4 cut(s)	16, 473, 920, 923
BshFI	GGCC	2 cut(s)	1017, 1049
BsiHKCI	CYCGRG	1 cut(s)	982
BsiSI	CCGG	1 cut(s)	120
BslFI	GGGAC	1 cut(s)	32
BslI	CCNNNNNNNGG	7 cut(s)	446, 482, 500, 846, 950, 1024, 1056
BsmFI	GGGAC	1 cut(s)	32
BsmI	GAATGC	3 cut(s)	158, 510, 525
BsnI	GGCC	2 cut(s)	1017, 1049
BsoBI	CYCGRG	1 cut(s)	982
Bsp119I	TTCGAA	1 cut(s)	1040
Bsp1407I	TGTACA	1 cut(s)	1010
Bsp143I	GATC	4 cut(s)	184, 260, 338, 436
Bsp19I	CCATGG	2 cut(s)	74, 946
BspACI	CCGC	2 cut(s)	509, 905
BspANI	GGCC	2 cut(s)	1017, 1049
BspCNI	CTCAG	1 cut(s)	600
BspHI	TCATGA	1 cut(s)	658
BspLI	GGNNCC	6 cut(s)	72, 118, 340, 438, 549, 883
BspPI	GGATC	4 cut(s)	333, 346, 431, 444
BspT104I	TTCGAA	1 cut(s)	1040
BsrDI	GCAATG	1 cut(s)	524
BsrGI	TGTACA	1 cut(s)	1010
BsrI	ACTGG	5 cut(s)	118, 342, 706, 754, 1072
BssECI	CCNNGG	3 cut(s)	74, 577, 946
BssMI	GATC	4 cut(s)	184, 260, 338, 436
BssNI	GRCGYC	1 cut(s)	1060
BssT1I	CCWWGG	3 cut(s)	74, 577, 946
Bst2UI	CCWGG	1 cut(s)	1046
Bst4CI	ACNGT	2 cut(s)	46, 355
BstACI	GRCGYC	1 cut(s)	1060
BstAUI	TGTACA	1 cut(s)	1010
BstBAI	YACGTR	1 cut(s)	605
BstBI	TTCGAA	1 cut(s)	1040
BstC8I	GCNNGC	6 cut(s)	333, 513, 941, 956, 960, 1019
BstDEI	CTNAG	1 cut(s)	587
BstDSI	CCRYGG	2 cut(s)	74, 946
BstENI	CCTNNNNNAGG	1 cut(s)	1054
BstF5I	GGATG	1 cut(s)	1068
BstHHI	GCGC	2 cut(s)	376, 892
BstKTI	GATC	4 cut(s)	187, 263, 341, 439
BstMBI	GATC	4 cut(s)	184, 260, 338, 436
BstMWI	GCNNNNNNNGC	3 cut(s)	722, 896, 905
BstNI	CCWGG	1 cut(s)	1046
BstNSI	RCATGY	4 cut(s)	38, 335, 515, 680
BstSCI	CCNGG	2 cut(s)	119, 1044
BstSFI	CTRYAG	1 cut(s)	351
BstV1I	GCAGC	4 cut(s)	16, 473, 920, 923
BstV2I	GAAGAC	1 cut(s)	601
BstX2I	RGATCY	2 cut(s)	338, 436
BstXI	CCANNNNNNTGG	1 cut(s)	947
BstYI	RGATCY	2 cut(s)	338, 436
BsuI	GTATCC	1 cut(s)	142
BsuRI	GGCC	2 cut(s)	1017, 1049
BtgI	CCRYGG	2 cut(s)	74, 946
BtsCI	GGATG	1 cut(s)	1068
BtsI	GCAGTG	1 cut(s)	64
BtsIMutI	CAGTG	4 cut(s)	64, 349, 360, 747
Cac8I	GCNNGC	6 cut(s)	333, 513, 941, 956, 960, 1019
CaiI	CAGNNNCTG	1 cut(s)	836
CciI	TCATGA	1 cut(s)	658
CfoI	GCGC	2 cut(s)	376, 892
Cfr13I	GGNCC	1 cut(s)	776
CseI	GACGC	1 cut(s)	1049
Csp6I	GTAC	4 cut(s)	347, 699, 765, 1011
CviAII	CATG	8 cut(s)	35, 75, 332, 512, 659, 677, 846, 947
CviQI	GTAC	4 cut(s)	347, 699, 765, 1011
DdeI	CTNAG	1 cut(s)	587
DpnI	GATC	4 cut(s)	186, 262, 340, 438
DpnII	GATC	4 cut(s)	184, 260, 338, 436
Eco130I	CCWWGG	3 cut(s)	74, 577, 946
Eco147I	AGGCCT	1 cut(s)	1049
Eco32I	GATATC	1 cut(s)	473
Eco47I	GGWCC	1 cut(s)	776
Eco57I	CTGAAG	1 cut(s)	36
Eco72I	CACGTG	1 cut(s)	605
Eco88I	CYCGRG	1 cut(s)	982
EcoNI	CCTNNNNNAGG	1 cut(s)	1054
EcoRI	GAATTC	1 cut(s)	850
EcoRII	CCWGG	1 cut(s)	1044
EcoRV	GATATC	1 cut(s)	473
EcoT14I	CCWWGG	3 cut(s)	74, 577, 946
ErhI	CCWWGG	3 cut(s)	74, 577, 946
FaeI	CATG	8 cut(s)	38, 78, 335, 515, 662, 680, 849, 950
FalI	AAGNNNNNCTT	4 cut(s)	603, 635, 1021, 1053
FaqI	GGGAC	1 cut(s)	32
FatI	CATG	8 cut(s)	34, 74, 331, 511, 658, 676, 845, 946
FbaI	TGATCA	1 cut(s)	184
Fnu4HI	GCNGC	6 cut(s)	5, 462, 509, 906, 934, 937
FokI	GGATG	1 cut(s)	1055
Fsp4HI	GCNGC	6 cut(s)	5, 462, 509, 906, 934, 937
FspBI	CTAG	1 cut(s)	516
FspI	TGCGCA	1 cut(s)	375
GlaI	GCGC	2 cut(s)	375, 891
GluI	GCNGC	6 cut(s)	5, 462, 509, 906, 934, 937
GsuI	CTGGAG	1 cut(s)	723
HaeIII	GGCC	2 cut(s)	1017, 1049
HapII	CCGG	1 cut(s)	120
HgaI	GACGC	1 cut(s)	1049
HhaI	GCGC	2 cut(s)	376, 892
Hin1I	GRCGYC	1 cut(s)	1060
Hin1II	CATG	8 cut(s)	38, 78, 335, 515, 662, 680, 849, 950
Hin6I	GCGC	2 cut(s)	374, 890
HinP1I	GCGC	2 cut(s)	374, 890
HincII	GTYRAC	1 cut(s)	866
HindII	GTYRAC	1 cut(s)	866
HinfI	GANTC	4 cut(s)	173, 239, 662, 833
HpaII	CCGG	1 cut(s)	120
HphI	GGTGA	1 cut(s)	743
Hpy166II	GTNNAC	2 cut(s)	347, 866
Hpy188I	TCNGA	6 cut(s)	16, 70, 172, 265, 817, 832
Hpy188III	TCNNGA	5 cut(s)	434, 533, 643, 659, 666
Hpy8I	GTNNAC	2 cut(s)	347, 866
HpyAV	CCTTC	3 cut(s)	681, 984, 995
HpyCH4III	ACNGT	2 cut(s)	46, 355
HpyCH4IV	ACGT	2 cut(s)	604, 709
HpyCH4V	TGCA	6 cut(s)	4, 566, 608, 716, 920, 958
HpyF10VI	GCNNNNNNNGC	3 cut(s)	722, 896, 905
HpyF3I	CTNAG	1 cut(s)	587
HpySE526I	ACGT	2 cut(s)	604, 709
Hsp92I	GRCGYC	1 cut(s)	1060
Hsp92II	CATG	8 cut(s)	38, 78, 335, 515, 662, 680, 849, 950
HspAI	GCGC	2 cut(s)	374, 890
Ksp22I	TGATCA	1 cut(s)	184
Kzo9I	GATC	4 cut(s)	184, 260, 338, 436
LmnI	GCTCC	1 cut(s)	547
Lsp1109I	GCAGC	4 cut(s)	16, 473, 920, 923
MaeI	CTAG	1 cut(s)	516
MaeII	ACGT	2 cut(s)	604, 709
MaeIII	GTNAC	1 cut(s)	401
MalI	GATC	4 cut(s)	186, 262, 340, 438
MboI	GATC	4 cut(s)	184, 260, 338, 436
MboII	GAAGA	6 cut(s)	135, 227, 606, 647, 1010, 1029
MflI	RGATCY	2 cut(s)	338, 436
MluCI	AATT	4 cut(s)	290, 301, 850, 913
MlyI	GAGTC	1 cut(s)	182
MmeI	TCCRAC	2 cut(s)	70, 159
MroXI	GAANNNNTTC	1 cut(s)	822
MslI	CAYNNNNRTG	3 cut(s)	321, 675, 915
MspI	CCGG	1 cut(s)	120
MspR9I	CCNGG	2 cut(s)	121, 1046
Mva1269I	GAATGC	3 cut(s)	158, 510, 525
MvaI	CCWGG	1 cut(s)	1046
MwoI	GCNNNNNNNGC	3 cut(s)	722, 896, 905
NciI	CCSGG	1 cut(s)	121
NcoI	CCATGG	2 cut(s)	74, 946
NdeII	GATC	4 cut(s)	184, 260, 338, 436
NlaIII	CATG	8 cut(s)	38, 78, 335, 515, 662, 680, 849, 950
NlaIV	GGNNCC	6 cut(s)	72, 118, 340, 438, 549, 883
NmuCI	GTSAC	1 cut(s)	401
NsbI	TGCGCA	1 cut(s)	375
NspI	RCATGY	4 cut(s)	38, 335, 515, 680
NspV	TTCGAA	1 cut(s)	1040
PaeI	GCATGC	2 cut(s)	335, 515
PagI	TCATGA	1 cut(s)	658
PceI	AGGCCT	1 cut(s)	1049
PctI	GAATGC	3 cut(s)	158, 510, 525
PdmI	GAANNNNTTC	1 cut(s)	822
PfeI	GAWTC	3 cut(s)	239, 662, 833
PkrI	GCNGC	6 cut(s)	6, 463, 510, 907, 935, 938
PleI	GAGTC	1 cut(s)	181
PmaCI	CACGTG	1 cut(s)	605
PmlI	CACGTG	1 cut(s)	605
PpsI	GAGTC	1 cut(s)	181
Ppu21I	YACGTR	1 cut(s)	605
Psp6I	CCWGG	1 cut(s)	1044
PspCI	CACGTG	1 cut(s)	605
PspGI	CCWGG	1 cut(s)	1044
PspN4I	GGNNCC	6 cut(s)	72, 118, 340, 438, 549, 883
PspPI	GGNCC	1 cut(s)	776
PstNI	CAGNNNCTG	1 cut(s)	836
PsuI	RGATCY	2 cut(s)	338, 436
RsaI	GTAC	4 cut(s)	348, 700, 766, 1012
RsaNI	GTAC	4 cut(s)	347, 699, 765, 1011
RseI	CAYNNNNRTG	3 cut(s)	321, 675, 915
SatI	GCNGC	6 cut(s)	5, 462, 509, 906, 934, 937
Sau3AI	GATC	4 cut(s)	184, 260, 338, 436
Sau96I	GGNCC	1 cut(s)	776
SchI	GAGTC	1 cut(s)	182
ScrFI	CCNGG	2 cut(s)	121, 1046
SfcI	CTRYAG	1 cut(s)	351
SfuI	TTCGAA	1 cut(s)	1040
SinI	GGWCC	1 cut(s)	776
SmiMI	CAYNNNNRTG	3 cut(s)	321, 675, 915
SmlI	CTYRAG	1 cut(s)	533
SmoI	CTYRAG	1 cut(s)	533
SphI	GCATGC	2 cut(s)	335, 515
Sse9I	AATT	4 cut(s)	290, 301, 850, 913
SseBI	AGGCCT	1 cut(s)	1049
SsiI	CCGC	2 cut(s)	509, 905
SspMI	CTAG	1 cut(s)	516
StuI	AGGCCT	1 cut(s)	1049
StyD4I	CCNGG	2 cut(s)	119, 1044
StyI	CCWWGG	3 cut(s)	74, 577, 946
TaaI	ACNGT	2 cut(s)	46, 355
TaiI	ACGT	2 cut(s)	607, 712
TaqI	TCGA	2 cut(s)	28, 1040
TasI	AATT	4 cut(s)	290, 301, 850, 913
TatI	WGTACW	1 cut(s)	1010
TauI	GCSGC	2 cut(s)	511, 908
TfiI	GAWTC	3 cut(s)	239, 662, 833
TscAI	CASTG	4 cut(s)	64, 349, 360, 754
TseFI	GTSAC	1 cut(s)	401
TseI	GCWGC	4 cut(s)	4, 461, 933, 936
Tsp45I	GTSAC	1 cut(s)	401
TspDTI	ATGAA	5 cut(s)	228, 647, 675, 795, 966
TspGWI	ACGGA	1 cut(s)	816
TspRI	CASTG	4 cut(s)	64, 349, 360, 754
VpaK11BI	GGWCC	1 cut(s)	776
XagI	CCTNNNNNAGG	1 cut(s)	1054
XapI	RAATTY	2 cut(s)	290, 850
XceI	RCATGY	4 cut(s)	38, 335, 515, 680
XcmI	CCANNNNNNNNNTGG	1 cut(s)	947
XmnI	GAANNNNTTC	1 cut(s)	822
XspI	CTAG	1 cut(s)	516

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.