MD03G1177900.v1.1
NAC Family

Inactive beta-amylase 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0004013
Physical Location & Seq
Reverse (-)
24473057 .. 24474061
1005 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1177900.v1.1.491

Sequence Viewer

Length: 324 bp
ATGGAATGGTACAGTGGTAGGTTGCTCCATCATGCAGATGATATTCTTGCAAAGGCAGCTAACATTCTGAAGAAATATCAAGAAAACAAGCAAACTTCAATTATATTGGCGGCTAAAATTGGTGGAATATATTGGTGGTATCAGGCGGTAGCACACCCTGCTGAACTTACAGCAGGATACTACAACACTGCTGTTAAAGATGGTTATGATCCTGTTGCTTCACTTTTGTCTCGTCATGGAGCTGCTTTGCATGTTCACTGCTTAGAAATGATGGATGGTGATAACCCGGCATCCTGTCTTTGTAGCCCAGAAGGATTACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_14

Protein Analysis

108

Amino Acids

11.79

Weight (kDa)

6.01

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_14 PF01373 1 - 106 7.4e-24 Glycosyl hydrolase family 14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 110, 146
AclWI GGATC 1 cut(s) 203
AcuI CTGAAG 1 cut(s) 89
AfaI GTAC 1 cut(s) 11
AgsI TTSAA 1 cut(s) 99
AluBI AGCT 2 cut(s) 59, 242
AluI AGCT 2 cut(s) 59, 242
Alw26I GTCTC 1 cut(s) 234
AlwI GGATC 1 cut(s) 203
ApeKI GCWGC 2 cut(s) 56, 242
AsuC2I CCSGG 1 cut(s) 287
AsuHPI GGTGA 1 cut(s) 290
BaeI ACNNNNGTAYC 1 cut(s) 34
BbvI GCAGC 2 cut(s) 68, 229
BccI CCATC 4 cut(s) 36, 194, 265, 269
BciVI GTATCC 1 cut(s) 170
BcnI CCSGG 1 cut(s) 287
BcoDI GTCTC 1 cut(s) 234
BfuI GTATCC 1 cut(s) 170
BisI GCNGC 3 cut(s) 57, 111, 243
BlsI GCNGC 3 cut(s) 58, 112, 244
Bme1390I CCNGG 1 cut(s) 287
BmrFI CCNGG 1 cut(s) 287
BmsI GCATC 1 cut(s) 299
BpuMI CCSGG 1 cut(s) 287
BseGI GGATG 2 cut(s) 280, 290
BseXI GCAGC 2 cut(s) 68, 229
BsiSI CCGG 1 cut(s) 287
BsmAI GTCTC 1 cut(s) 234
Bsp143I GATC 1 cut(s) 208
BspACI CCGC 2 cut(s) 110, 146
BspPI GGATC 1 cut(s) 203
BssMI GATC 1 cut(s) 208
Bst4CI ACNGT 1 cut(s) 14
BstAPI GCANNNNNTGC 1 cut(s) 158
BstDEI CTNAG 1 cut(s) 262
BstF5I GGATG 2 cut(s) 280, 290
BstKTI GATC 1 cut(s) 211
BstMAI GTCTC 1 cut(s) 234
BstMBI GATC 1 cut(s) 208
BstMWI GCNNNNNNNGC 2 cut(s) 56, 158
BstNSI RCATGY 1 cut(s) 254
BstSCI CCNGG 1 cut(s) 285
BstV1I GCAGC 2 cut(s) 68, 229
BsuI GTATCC 1 cut(s) 170
BtsCI GGATG 2 cut(s) 280, 290
BtsI GCAGTG 2 cut(s) 186, 256
BtsIMutI CAGTG 3 cut(s) 19, 186, 256
Csp6I GTAC 1 cut(s) 10
CviAII CATG 3 cut(s) 32, 236, 251
CviJI RGCY 4 cut(s) 59, 113, 242, 306
CviKI_1 RGCY 4 cut(s) 59, 113, 242, 306
CviQI GTAC 1 cut(s) 10
DdeI CTNAG 1 cut(s) 262
DpnI GATC 1 cut(s) 210
DpnII GATC 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 89
FaeI CATG 3 cut(s) 35, 239, 254
FaiI YATR 6 cut(s) 33, 104, 130, 207, 237, 252
FatI CATG 3 cut(s) 31, 235, 250
Fnu4HI GCNGC 3 cut(s) 57, 111, 243
FokI GGATG 2 cut(s) 277, 287
Fsp4HI GCNGC 3 cut(s) 57, 111, 243
GluI GCNGC 3 cut(s) 57, 111, 243
HapII CCGG 1 cut(s) 287
Hin1II CATG 3 cut(s) 35, 239, 254
HpaII CCGG 1 cut(s) 287
HphI GGTGA 1 cut(s) 290
Hpy166II GTNNAC 1 cut(s) 256
Hpy188I TCNGA 1 cut(s) 69
Hpy188III TCNNGA 1 cut(s) 80
Hpy8I GTNNAC 1 cut(s) 256
HpyAV CCTTC 1 cut(s) 305
HpyCH4III ACNGT 1 cut(s) 14
HpyCH4V TGCA 3 cut(s) 35, 50, 250
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 158
HpyF3I CTNAG 1 cut(s) 262
Hsp92II CATG 3 cut(s) 35, 239, 254
Kzo9I GATC 1 cut(s) 208
LmnI GCTCC 2 cut(s) 30, 239
LpnPI CCDG 6 cut(s) 128, 159, 171, 225, 300, 307
Lsp1109I GCAGC 2 cut(s) 68, 229
LweI GCATC 1 cut(s) 299
MalI GATC 1 cut(s) 210
MboI GATC 1 cut(s) 208
MboII GAAGA 1 cut(s) 82
MluCI AATT 2 cut(s) 99, 117
MseI TTAA 1 cut(s) 195
MslI CAYNNNNRTG 1 cut(s) 36
MspI CCGG 1 cut(s) 287
MspR9I CCNGG 1 cut(s) 287
MwoI GCNNNNNNNGC 2 cut(s) 56, 158
NciI CCSGG 1 cut(s) 287
NdeII GATC 1 cut(s) 208
NlaIII CATG 3 cut(s) 35, 239, 254
NspI RCATGY 1 cut(s) 254
PkrI GCNGC 3 cut(s) 58, 112, 244
RsaI GTAC 1 cut(s) 11
RsaNI GTAC 1 cut(s) 10
RseI CAYNNNNRTG 1 cut(s) 36
SaqAI TTAA 1 cut(s) 195
SatI GCNGC 3 cut(s) 57, 111, 243
Sau3AI GATC 1 cut(s) 208
ScrFI CCNGG 1 cut(s) 287
SetI ASST 3 cut(s) 23, 61, 244
SfaNI GCATC 1 cut(s) 299
SmiMI CAYNNNNRTG 1 cut(s) 36
Sse9I AATT 2 cut(s) 99, 117
SsiI CCGC 2 cut(s) 110, 146
StyD4I CCNGG 1 cut(s) 285
TaaI ACNGT 1 cut(s) 14
TasI AATT 2 cut(s) 99, 117
TauI GCSGC 1 cut(s) 113
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TscAI CASTG 3 cut(s) 19, 193, 263
TseI GCWGC 2 cut(s) 56, 242
TspRI CASTG 3 cut(s) 19, 193, 263
XceI RCATGY 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.