Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0004013

Physical Location & Seq

Genomic Coordinates Reverse (-)

24749624 .. 24751779

Gene Structure 2156 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1180300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 777 bp

ATGATGCCTGTCGATGCCTTCTGCAGTGATGGTACGGGGCGTCCAAGGATTATGAAAATCAAGGCCTCAACTGTAGCTCTAAAAGCACTCAAGTTGGCAGGTGTCCACGGAATTGCAGTTGAGGTTTGGTGGGGAATTGTAGAGCGTTTCTCTCCTCTTGCATATGATTGGTCTCTATATGAAGAGCTTTTCAATCTGATTTCTGACTCAGATTTGAAGTTGCATGTTGCCTTGTCTTTTCACTCAAACGTTAACTCTTCTTCTAGCAGAAAAGGGGGTGTAACTCTTCCACTTAGGATCATAGAGATTGGTGATCAGAATAAGCATATATATTATTGGGACCAAAATGGATTTTCCAATGATGACTATCTTACACTGAGAGTTGACCATGTTCCTCTCTTTTGCGGACGGACTGCCCTCCAATGTTATGAAGATTTCATGTCAAGTTTTGCTAAGAAATTTGAATTCTTAATCGGAACTGTAATAGAAGAAATTAGTGTTGGTCATGGTCCTTCTGGTGAACTTAAATATCCTGCACATCCTTTTGGTGATGGTAGATGGAAGTTTCCTGGAATCGGTGAATTCCAGTGTTATGACAAGTACATGATGGGTGACCTAAAGATGGCTGCACGCAAGGAAGGAAAACCTCAGTGGGAAGAGAAGGGACCACAAAAGACTGGCTGTTACAACAGTCTCCCATCTGAAGTTCCTTTCTTTGGAGAAGGAGAGGGAAGCTTTCTTTCTGATTATGGGTGTTTTTTCCTTGTACGAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005976 GO:0005982 GO:0005983 GO:0006073 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009251 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044237 GO:0044238 GO:0044247 GO:0044248 GO:0044260 GO:0044262 GO:0044264 GO:0044275 GO:0044424 GO:0044444 GO:0044464 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00500 map00500

Pfam Domains

Protein Families

Glyco_hydro_14

Protein Analysis

259

Amino Acids

29.07

Weight (kDa)

5.82

Isoelectric Point (pI)

39.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_14	PF01373	1 - 255	7e-62	Glycosyl hydrolase family 14

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004013)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27420
fragaria_vesca	FvH4_3g20680 FvH4_3g29210
malus_domestica	MD03G1138200.v1.1 MD03G1177900.v1.1 MD03G1180300.v1.1 MD03G1181200.v1.1 MD03G1182200.v1.1 MD03G1222900.v1.1 MD11G1240300.v1.1
prunus_persica	Prupe.4G186700_v2.0.a1 Prupe.8G022900_v2.0.a1
pyrus_communis	pycom03g13470 pycom11g21230
rosa_chinensis	RchiOBHm_Chr5g0034741 RchiOBHm_Chr5g0053511 RchiOBHm_Chr5g0053631
rosa_laevigata	RLG00000033574 RLG00000034891
rosa_multiflora	Rmu_sc0002460.1_g000057 Rmu_sc0003228.1_g000001
rosa_roxburghii	Rroxscaffold_1G00045590
rosa_rugosa	Rorug05G0147900
rosa_samantha	Rh5AG240600 Rh5AG351700 Rh5AG352400 Rh5BG240700 Rh5BG364000 Rh5CG271700 Rh5DG249000 Rh5DG378600
rosa_wichuraiana	Rw5G021980 Rw5G033270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	89
Acc36I	ACCTGC	1 cut(s)	89
AciI	CCGC	1 cut(s)	405
AclI	AACGTT	1 cut(s)	249
AclWI	GGATC	1 cut(s)	305
AcsI	RAATTY	3 cut(s)	458, 464, 581
AcuI	CTGAAG	1 cut(s)	723
AcyI	GRCGYC	1 cut(s)	40
AfaI	GTAC	3 cut(s)	34, 602, 768
AfiI	CCNNNNNNNGG	3 cut(s)	575, 622, 716
AgsI	TTSAA	3 cut(s)	193, 217, 464
AjnI	CCWGG	1 cut(s)	568
AjuI	GAANNNNNNNTTGG	2 cut(s)	483, 515
AluBI	AGCT	3 cut(s)	77, 187, 735
AluI	AGCT	3 cut(s)	77, 187, 735
Alw26I	GTCTC	2 cut(s)	177, 698
AlwI	GGATC	1 cut(s)	305
AoxI	GGCC	1 cut(s)	63
ApeKI	GCWGC	1 cut(s)	626
ApoI	RAATTY	3 cut(s)	458, 464, 581
AspS9I	GGNCC	3 cut(s)	340, 509, 665
AsuHPI	GGTGA	5 cut(s)	323, 530, 560, 590, 623
AvaII	GGWCC	3 cut(s)	340, 509, 665
BbvI	GCAGC	1 cut(s)	613
BccI	CCATC	6 cut(s)	23, 545, 552, 601, 616, 706
BciT130I	CCWGG	1 cut(s)	570
BclI	TGATCA	1 cut(s)	313
BcoDI	GTCTC	2 cut(s)	177, 698
BfaI	CTAG	1 cut(s)	264
BfmI	CTRYAG	2 cut(s)	22, 72
BfuAI	ACCTGC	1 cut(s)	89
BisI	GCNGC	1 cut(s)	627
BlsI	GCNGC	1 cut(s)	628
Bme1390I	CCNGG	1 cut(s)	570
Bme18I	GGWCC	3 cut(s)	340, 509, 665
BmgT120I	GGNCC	3 cut(s)	340, 509, 665
BmiI	GGNNCC	2 cut(s)	341, 666
BmrFI	CCNGG	1 cut(s)	570
BmsI	GCATC	1 cut(s)	4
BplI	GAGNNNNNCTC	2 cut(s)	134, 166
BpuEI	CTTGAG	1 cut(s)	74
BsaBI	GATNNNNATC	1 cut(s)	366
BsaHI	GRCGYC	1 cut(s)	40
BsaI	GGTCTC	1 cut(s)	177
BsaJI	CCNNGG	2 cut(s)	44, 106
Bsc4I	CCNNNNNNNGG	3 cut(s)	575, 622, 716
Bse1I	ACTGG	2 cut(s)	586, 682
Bse8I	GATNNNNATC	1 cut(s)	366
BseBI	CCWGG	1 cut(s)	570
BseDI	CCNNGG	2 cut(s)	44, 106
BseGI	GGATG	1 cut(s)	538
BseJI	GATNNNNATC	1 cut(s)	366
BseLI	CCNNNNNNNGG	3 cut(s)	575, 622, 716
BseMII	CTCAG	3 cut(s)	222, 368, 662
BseNI	ACTGG	2 cut(s)	586, 682
BseRI	GAGGAG	1 cut(s)	144
BseXI	GCAGC	1 cut(s)	613
BsgI	GTGCAG	2 cut(s)	519, 612
BshFI	GGCC	1 cut(s)	65
BslFI	GGGAC	2 cut(s)	353, 678
BslI	CCNNNNNNNGG	3 cut(s)	575, 622, 716
BsmAI	GTCTC	2 cut(s)	177, 698
BsmFI	GGGAC	2 cut(s)	353, 678
BsnI	GGCC	1 cut(s)	65
Bso31I	GGTCTC	1 cut(s)	177
Bsp143I	GATC	2 cut(s)	297, 313
BspACI	CCGC	1 cut(s)	405
BspANI	GGCC	1 cut(s)	65
BspCNI	CTCAG	3 cut(s)	221, 369, 661
BspLI	GGNNCC	2 cut(s)	341, 666
BspMAI	CTGCAG	1 cut(s)	26
BspMI	ACCTGC	1 cut(s)	89
BspPI	GGATC	1 cut(s)	305
BspQI	GCTCTTC	1 cut(s)	177
BspTNI	GGTCTC	1 cut(s)	177
BsrI	ACTGG	2 cut(s)	586, 682
BssECI	CCNNGG	2 cut(s)	44, 106
BssMI	GATC	2 cut(s)	297, 313
BssNI	GRCGYC	1 cut(s)	40
BssT1I	CCWWGG	1 cut(s)	44
Bst2UI	CCWGG	1 cut(s)	570
Bst4CI	ACNGT	3 cut(s)	73, 481, 692
Bst6I	CTCTTC	4 cut(s)	177, 262, 291, 651
BstACI	GRCGYC	1 cut(s)	40
BstC8I	GCNNGC	1 cut(s)	631
BstDEI	CTNAG	5 cut(s)	208, 293, 377, 453, 648
BstDSI	CCRYGG	1 cut(s)	106
BstEII	GGTNACC	1 cut(s)	611
BstF5I	GGATG	1 cut(s)	538
BstKTI	GATC	2 cut(s)	300, 316
BstMAI	GTCTC	2 cut(s)	177, 698
BstMBI	GATC	2 cut(s)	297, 313
BstMWI	GCNNNNNNNGC	1 cut(s)	83
BstNI	CCWGG	1 cut(s)	570
BstNSI	RCATGY	1 cut(s)	227
BstPI	GGTNACC	1 cut(s)	611
BstSCI	CCNGG	1 cut(s)	568
BstSFI	CTRYAG	2 cut(s)	22, 72
BstV1I	GCAGC	1 cut(s)	613
BsuRI	GGCC	1 cut(s)	65
BtgI	CCRYGG	1 cut(s)	106
BtsCI	GGATG	1 cut(s)	538
BtsI	GCAGTG	1 cut(s)	31
BtsIMutI	CAGTG	4 cut(s)	31, 374, 593, 656
BveI	ACCTGC	1 cut(s)	89
Cac8I	GCNNGC	1 cut(s)	631
Cfr13I	GGNCC	3 cut(s)	340, 509, 665
CseI	GACGC	1 cut(s)	29
Csp6I	GTAC	3 cut(s)	33, 601, 767
CviAII	CATG	5 cut(s)	224, 389, 439, 506, 604
CviJI	RGCY	6 cut(s)	65, 77, 187, 626, 681, 735
CviKI_1	RGCY	6 cut(s)	65, 77, 187, 626, 681, 735
CviQI	GTAC	3 cut(s)	33, 601, 767
DdeI	CTNAG	5 cut(s)	208, 293, 377, 453, 648
DpnI	GATC	2 cut(s)	299, 315
DpnII	GATC	2 cut(s)	297, 313
Eam1104I	CTCTTC	4 cut(s)	177, 262, 291, 651
EarI	CTCTTC	4 cut(s)	177, 262, 291, 651
Eco130I	CCWWGG	1 cut(s)	44
Eco147I	AGGCCT	1 cut(s)	65
Eco31I	GGTCTC	1 cut(s)	177
Eco47I	GGWCC	3 cut(s)	340, 509, 665
Eco57I	CTGAAG	1 cut(s)	723
Eco91I	GGTNACC	1 cut(s)	611
EcoO65I	GGTNACC	1 cut(s)	611
EcoRI	GAATTC	2 cut(s)	464, 581
EcoRII	CCWGG	1 cut(s)	568
EcoT14I	CCWWGG	1 cut(s)	44
ErhI	CCWWGG	1 cut(s)	44
FaeI	CATG	5 cut(s)	227, 392, 442, 509, 607
FaqI	GGGAC	2 cut(s)	353, 678
FatI	CATG	5 cut(s)	223, 388, 438, 505, 603
FauNDI	CATATG	1 cut(s)	163
FbaI	TGATCA	1 cut(s)	313
Fnu4HI	GCNGC	1 cut(s)	627
FokI	GGATG	1 cut(s)	525
Fsp4HI	GCNGC	1 cut(s)	627
FspBI	CTAG	1 cut(s)	264
GluI	GCNGC	1 cut(s)	627
HaeIII	GGCC	1 cut(s)	65
HgaI	GACGC	1 cut(s)	29
Hin1I	GRCGYC	1 cut(s)	40
Hin1II	CATG	5 cut(s)	227, 392, 442, 509, 607
HincII	GTYRAC	2 cut(s)	253, 385
HindII	GTYRAC	2 cut(s)	253, 385
HindIII	AAGCTT	1 cut(s)	733
HinfI	GANTC	2 cut(s)	206, 573
HpaI	GTTAAC	1 cut(s)	253
HphI	GGTGA	5 cut(s)	323, 530, 560, 590, 623
Hpy166II	GTNNAC	4 cut(s)	106, 253, 385, 521
Hpy188I	TCNGA	7 cut(s)	198, 205, 211, 318, 476, 703, 745
Hpy8I	GTNNAC	4 cut(s)	106, 253, 385, 521
HpyAV	CCTTC	5 cut(s)	28, 522, 632, 655, 716
HpyCH4III	ACNGT	3 cut(s)	73, 481, 692
HpyCH4IV	ACGT	1 cut(s)	249
HpyCH4V	TGCA	6 cut(s)	24, 116, 161, 223, 536, 629
HpyF10VI	GCNNNNNNNGC	1 cut(s)	83
HpyF3I	CTNAG	5 cut(s)	208, 293, 377, 453, 648
HpySE526I	ACGT	1 cut(s)	249
Hsp92I	GRCGYC	1 cut(s)	40
Hsp92II	CATG	5 cut(s)	227, 392, 442, 509, 607
Ksp22I	TGATCA	1 cut(s)	313
KspAI	GTTAAC	1 cut(s)	253
Kzo9I	GATC	2 cut(s)	297, 313
LguI	GCTCTTC	1 cut(s)	177
LpnPI	CCDG	8 cut(s)	21, 84, 501, 546, 555, 582, 599, 663
Lsp1109I	GCAGC	1 cut(s)	613
LweI	GCATC	1 cut(s)	4
MaeI	CTAG	1 cut(s)	264
MaeII	ACGT	1 cut(s)	249
MaeIII	GTNAC	3 cut(s)	280, 611, 683
MalI	GATC	2 cut(s)	299, 315
MboI	GATC	2 cut(s)	297, 313
MboII	GAAGA	7 cut(s)	194, 249, 252, 278, 443, 500, 668
MluCI	AATT	6 cut(s)	111, 135, 458, 464, 492, 581
MlyI	GAGTC	1 cut(s)	200
MnlI	CCTC	7 cut(s)	76, 115, 165, 405, 428, 657, 721
MseI	TTAA	3 cut(s)	252, 470, 525
MspR9I	CCNGG	1 cut(s)	570
MvaI	CCWGG	1 cut(s)	570
MwoI	GCNNNNNNNGC	1 cut(s)	83
NdeI	CATATG	1 cut(s)	163
NdeII	GATC	2 cut(s)	297, 313
NlaIII	CATG	5 cut(s)	227, 392, 442, 509, 607
NlaIV	GGNNCC	2 cut(s)	341, 666
NmuCI	GTSAC	1 cut(s)	611
NspI	RCATGY	1 cut(s)	227
PaqCI	CACCTGC	1 cut(s)	89
PceI	AGGCCT	1 cut(s)	65
PciSI	GCTCTTC	1 cut(s)	177
PfeI	GAWTC	1 cut(s)	573
PfoI	TCCNGGA	1 cut(s)	568
PkrI	GCNGC	1 cut(s)	628
PleI	GAGTC	1 cut(s)	200
PpsI	GAGTC	1 cut(s)	200
Psp1406I	AACGTT	1 cut(s)	249
Psp6I	CCWGG	1 cut(s)	568
PspEI	GGTNACC	1 cut(s)	611
PspGI	CCWGG	1 cut(s)	568
PspN4I	GGNNCC	2 cut(s)	341, 666
PspPI	GGNCC	3 cut(s)	340, 509, 665
PstI	CTGCAG	1 cut(s)	26
RsaI	GTAC	3 cut(s)	34, 602, 768
RsaNI	GTAC	3 cut(s)	33, 601, 767
SapI	GCTCTTC	1 cut(s)	177
SaqAI	TTAA	3 cut(s)	252, 470, 525
SatI	GCNGC	1 cut(s)	627
Sau3AI	GATC	2 cut(s)	297, 313
Sau96I	GGNCC	3 cut(s)	340, 509, 665
SchI	GAGTC	1 cut(s)	200
ScrFI	CCNGG	1 cut(s)	570
SetI	ASST	8 cut(s)	79, 103, 126, 189, 252, 618, 649, 737
SfaNI	GCATC	1 cut(s)	4
SfcI	CTRYAG	2 cut(s)	22, 72
SinI	GGWCC	3 cut(s)	340, 509, 665
SmlI	CTYRAG	1 cut(s)	89
SmoI	CTYRAG	1 cut(s)	89
Sse9I	AATT	6 cut(s)	111, 135, 458, 464, 492, 581
SseBI	AGGCCT	1 cut(s)	65
SsiI	CCGC	1 cut(s)	405
SspMI	CTAG	1 cut(s)	264
StuI	AGGCCT	1 cut(s)	65
StyD4I	CCNGG	1 cut(s)	568
StyI	CCWWGG	1 cut(s)	44
TaaI	ACNGT	3 cut(s)	73, 481, 692
TaiI	ACGT	1 cut(s)	252
TaqI	TCGA	1 cut(s)	12
TasI	AATT	6 cut(s)	111, 135, 458, 464, 492, 581
TatI	WGTACW	1 cut(s)	600
TfiI	GAWTC	1 cut(s)	573
Tru1I	TTAA	3 cut(s)	252, 470, 525
Tru9I	TTAA	3 cut(s)	252, 470, 525
TscAI	CASTG	4 cut(s)	31, 381, 593, 656
TseFI	GTSAC	1 cut(s)	611
TseI	GCWGC	1 cut(s)	626
Tsp45I	GTSAC	1 cut(s)	611
TspDTI	ATGAA	4 cut(s)	68, 195, 427, 444
TspGWI	ACGGA	2 cut(s)	123, 424
TspRI	CASTG	4 cut(s)	31, 381, 593, 656
VpaK11BI	GGWCC	3 cut(s)	340, 509, 665
XapI	RAATTY	3 cut(s)	458, 464, 581
XceI	RCATGY	1 cut(s)	227
XspI	CTAG	1 cut(s)	264

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.