MD03G1184800.v1.1

transposition, RNA-mediated

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014977
Physical Location & Seq
Forward (+)
25137114 .. 25139508
2395 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1184800.v1.1.491

Sequence Viewer

Length: 624 bp
ATGACCCTGAGTTGTGTTCTGAAAGTAAGCATCAATTGTGAAGCATGCAGGAAAAAGATCTATGAAGTCTTGCAGAATATTTATGGTGTGTATAAAATTAACATAGATGCAGAGGGAGGAACGGTAAAAGTTTCAGGGAAAGTAAATCCAAGTACACTTCTAATGGTGTTGGAAGGGTCAGGGAAACATGCAGAAGTAAAAAGTGTTACGTTCGACGGTGAGGTCAAGAACGATTGGGGTGGGGGATACGATTATTTTGGAGAGGCTGGATATGGTTATGGTTATAACCCTTATGGAGTGGCGCCACCGCCATTTTATCCCTATCCGCCGTATGGAGGGTATGACTACCACTGGTATGATCCCGGTCGTCTCAAAATGATGCCATTGCCAGCGCTGCCCCCACCACCATTGGCGTTACCTTCGTCATCGCCACCGGTACCACAGCAGTCTCAACCACCACTGCCACTACAATTACAATCACAATCAGTGCCACCACAACCACCAAAAACACCGGCACCACCAAATCAGGCGCCACGACCACCGCCACCGGCAGCCAATGCTGCACAGACAATAGCGAAACCAGCGGCCAAATCGGATAAGAAATCTAAGTGTTGTATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

208

Amino Acids

22.3

Weight (kDa)

8.84

Isoelectric Point (pI)

69.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 8 - 63 2.8e-09 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014977)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13140
fragaria_vesca FvH4_3g29421
malus_domestica MD03G1184600.v1.1 MD03G1184800.v1.1 MD11G1201200.v1.1
prunus_persica Prupe.4G241700_v2.0.a1 Prupe.5G052600_v2.0.a1
pyrus_communis pycom11g17370
rosa_chinensis RchiOBHm_Chr5g0053851
rosa_multiflora Rmu_sc0001759.1_g000008
rosa_roxburghii Rroxscaffold_1G00026750
rosa_samantha Rh5AG353500 Rh5BG365100 Rh5CG390200
rosa_wichuraiana Rw0G002130 Rw0G009540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 285
AasI GACNNNNNNGTC 1 cut(s) 221
Acc65I GGTACC 1 cut(s) 436
AccB1I GGYRCC 4 cut(s) 301, 436, 514, 529
AciI CCGC 4 cut(s) 308, 326, 542, 584
AclWI GGATC 1 cut(s) 353
AcoI YGGCCR 1 cut(s) 585
AcyI GRCGYC 2 cut(s) 302, 530
AfaI GTAC 2 cut(s) 154, 438
AfeI AGCGCT 1 cut(s) 393
AfiI CCNNNNNNNGG 2 cut(s) 332, 335
AgeI ACCGGT 1 cut(s) 433
Alw26I GTCTC 2 cut(s) 374, 453
AlwI GGATC 1 cut(s) 353
Aor51HI AGCGCT 1 cut(s) 393
AoxI GGCC 1 cut(s) 585
ApeKI GCWGC 3 cut(s) 394, 551, 560
AsiGI ACCGGT 1 cut(s) 433
Asp718I GGTACC 1 cut(s) 436
AspLEI GCGC 3 cut(s) 304, 394, 532
AsuC2I CCSGG 1 cut(s) 363
AsuHPI GGTGA 1 cut(s) 230
BanI GGYRCC 4 cut(s) 301, 436, 514, 529
BbvI GCAGC 3 cut(s) 381, 547, 563
BceAI ACGGC 1 cut(s) 313
BciVI GTATCC 1 cut(s) 239
BcnI CCSGG 1 cut(s) 363
BcoDI GTCTC 2 cut(s) 374, 453
BfoI RGCGCY 3 cut(s) 305, 395, 533
BfuI GTATCC 1 cut(s) 239
BglII AGATCT 1 cut(s) 57
BisI GCNGC 4 cut(s) 395, 552, 561, 585
BlsI GCNGC 4 cut(s) 396, 553, 562, 586
Bme1390I CCNGG 1 cut(s) 363
BmiI GGNNCC 4 cut(s) 303, 438, 516, 531
BmrFI CCNGG 1 cut(s) 363
BmsI GCATC 3 cut(s) 39, 97, 369
BpuMI CCSGG 1 cut(s) 363
BsaHI GRCGYC 2 cut(s) 302, 530
BsaWI WCCGGW 1 cut(s) 433
BsaXI ACNNNNNCTCC 2 cut(s) 108, 138
Bsc4I CCNNNNNNNGG 2 cut(s) 332, 335
Bse118I RCCGGY 3 cut(s) 433, 511, 547
Bse1I ACTGG 1 cut(s) 356
Bse3DI GCAATG 1 cut(s) 383
BseLI CCNNNNNNNGG 2 cut(s) 332, 335
BseMI GCAATG 1 cut(s) 383
BseNI ACTGG 1 cut(s) 356
BseXI GCAGC 3 cut(s) 381, 547, 563
BsgI GTGCAG 1 cut(s) 546
Bsh1285I CGRYCG 1 cut(s) 367
BshFI GGCC 1 cut(s) 587
BshNI GGYRCC 4 cut(s) 301, 436, 514, 529
BshTI ACCGGT 1 cut(s) 433
BsiEI CGRYCG 1 cut(s) 367
BsiSI CCGG 4 cut(s) 363, 434, 512, 548
BslI CCNNNNNNNGG 2 cut(s) 332, 335
BsmAI GTCTC 2 cut(s) 374, 453
BsmBI CGTCTC 1 cut(s) 374
BsnI GGCC 1 cut(s) 587
Bsp143I GATC 2 cut(s) 57, 358
BspACI CCGC 4 cut(s) 308, 326, 542, 584
BspANI GGCC 1 cut(s) 587
BspLI GGNNCC 4 cut(s) 303, 438, 516, 531
BspPI GGATC 1 cut(s) 353
BspT107I GGYRCC 4 cut(s) 301, 436, 514, 529
BsrDI GCAATG 1 cut(s) 383
BsrFI RCCGGY 3 cut(s) 433, 511, 547
BsrI ACTGG 1 cut(s) 356
BssAI RCCGGY 3 cut(s) 433, 511, 547
BssMI GATC 2 cut(s) 57, 358
BssNI GRCGYC 2 cut(s) 302, 530
Bst4CI ACNGT 2 cut(s) 124, 218
BstACI GRCGYC 2 cut(s) 302, 530
BstAPI GCANNNNNTGC 1 cut(s) 557
BstC8I GCNNGC 2 cut(s) 46, 390
BstDEI CTNAG 2 cut(s) 8, 606
BstH2I RGCGCY 3 cut(s) 305, 395, 533
BstHHI GCGC 3 cut(s) 304, 394, 532
BstKTI GATC 2 cut(s) 60, 361
BstMAI GTCTC 2 cut(s) 374, 453
BstMBI GATC 2 cut(s) 57, 358
BstMCI CGRYCG 1 cut(s) 367
BstMWI GCNNNNNNNGC 4 cut(s) 394, 557, 560, 581
BstNSI RCATGY 2 cut(s) 48, 191
BstSCI CCNGG 1 cut(s) 361
BstV1I GCAGC 3 cut(s) 381, 547, 563
BstX2I RGATCY 1 cut(s) 57
BstYI RGATCY 1 cut(s) 57
BsuI GTATCC 1 cut(s) 239
BsuRI GGCC 1 cut(s) 587
BtgZI GCGATG 1 cut(s) 411
BtsI GCAGTG 1 cut(s) 458
BtsIMutI CAGTG 3 cut(s) 349, 458, 492
Cac8I GCNNGC 2 cut(s) 46, 390
CfoI GCGC 3 cut(s) 304, 394, 532
Cfr10I RCCGGY 3 cut(s) 433, 511, 547
Csp6I GTAC 2 cut(s) 153, 437
CspAI ACCGGT 1 cut(s) 433
CspCI CAANNNNNGTGG 2 cut(s) 390, 425
CviAII CATG 3 cut(s) 45, 188, 619
CviJI RGCY 3 cut(s) 266, 554, 587
CviKI_1 RGCY 3 cut(s) 266, 554, 587
CviQI GTAC 2 cut(s) 153, 437
DdeI CTNAG 2 cut(s) 8, 606
DinI GGCGCC 2 cut(s) 303, 531
DpnI GATC 2 cut(s) 59, 360
DpnII GATC 2 cut(s) 57, 358
DrdI GACNNNNNNGTC 1 cut(s) 221
DseDI GACNNNNNNGTC 1 cut(s) 221
EaeI YGGCCR 1 cut(s) 585
EciI GGCGGA 1 cut(s) 315
Eco47III AGCGCT 1 cut(s) 393
EgeI GGCGCC 2 cut(s) 303, 531
EheI GGCGCC 2 cut(s) 303, 531
Esp3I CGTCTC 1 cut(s) 374
FaeI CATG 3 cut(s) 48, 191, 622
FatI CATG 3 cut(s) 44, 187, 618
Fnu4HI GCNGC 4 cut(s) 395, 552, 561, 585
Fsp4HI GCNGC 4 cut(s) 395, 552, 561, 585
GlaI GCGC 3 cut(s) 303, 393, 531
GluI GCNGC 4 cut(s) 395, 552, 561, 585
HaeII RGCGCY 3 cut(s) 305, 395, 533
HaeIII GGCC 1 cut(s) 587
HapII CCGG 4 cut(s) 363, 434, 512, 548
HhaI GCGC 3 cut(s) 304, 394, 532
Hin1I GRCGYC 2 cut(s) 302, 530
Hin1II CATG 3 cut(s) 48, 191, 622
Hin6I GCGC 3 cut(s) 302, 392, 530
HinP1I GCGC 3 cut(s) 302, 392, 530
HpaII CCGG 4 cut(s) 363, 434, 512, 548
HphI GGTGA 1 cut(s) 230
Hpy166II GTNNAC 1 cut(s) 155
Hpy188I TCNGA 2 cut(s) 21, 595
Hpy188III TCNNGA 1 cut(s) 226
Hpy8I GTNNAC 1 cut(s) 155
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 2 cut(s) 167, 429
HpyCH4III ACNGT 2 cut(s) 124, 218
HpyCH4IV ACGT 1 cut(s) 209
HpyCH4V TGCA 5 cut(s) 48, 73, 110, 191, 563
HpyF10VI GCNNNNNNNGC 4 cut(s) 394, 557, 560, 581
HpyF3I CTNAG 2 cut(s) 8, 606
HpySE526I ACGT 1 cut(s) 209
Hsp92I GRCGYC 2 cut(s) 302, 530
Hsp92II CATG 3 cut(s) 48, 191, 622
HspAI GCGC 3 cut(s) 302, 392, 530
KasI GGCGCC 2 cut(s) 301, 529
KpnI GGTACC 1 cut(s) 440
Kzo9I GATC 2 cut(s) 57, 358
Lsp1109I GCAGC 3 cut(s) 381, 547, 563
LweI GCATC 3 cut(s) 39, 97, 369
MaeII ACGT 1 cut(s) 209
MaeIII GTNAC 2 cut(s) 205, 414
MalI GATC 2 cut(s) 59, 360
MboI GATC 2 cut(s) 57, 358
MfeI CAATTG 1 cut(s) 34
MflI RGATCY 1 cut(s) 57
MluCI AATT 3 cut(s) 34, 96, 470
Mly113I GGCGCC 2 cut(s) 302, 530
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 5 cut(s) 106, 110, 214, 256, 329
MseI TTAA 1 cut(s) 99
MslI CAYNNNNRTG 1 cut(s) 354
MspA1I CMGCKG 1 cut(s) 584
MspI CCGG 4 cut(s) 363, 434, 512, 548
MspR9I CCNGG 1 cut(s) 363
MunI CAATTG 1 cut(s) 34
MwoI GCNNNNNNNGC 4 cut(s) 394, 557, 560, 581
NarI GGCGCC 2 cut(s) 302, 530
NciI CCSGG 1 cut(s) 363
NdeII GATC 2 cut(s) 57, 358
NlaIII CATG 3 cut(s) 48, 191, 622
NlaIV GGNNCC 4 cut(s) 303, 438, 516, 531
NspI RCATGY 2 cut(s) 48, 191
PaeI GCATGC 1 cut(s) 48
PinAI ACCGGT 1 cut(s) 433
PkrI GCNGC 4 cut(s) 396, 553, 562, 586
PluTI GGCGCC 2 cut(s) 305, 533
PsiI TTATAA 1 cut(s) 285
PspN4I GGNNCC 4 cut(s) 303, 438, 516, 531
PsuI RGATCY 1 cut(s) 57
RsaI GTAC 2 cut(s) 154, 438
RsaNI GTAC 2 cut(s) 153, 437
RseI CAYNNNNRTG 1 cut(s) 354
SaqAI TTAA 1 cut(s) 99
SatI GCNGC 4 cut(s) 395, 552, 561, 585
Sau3AI GATC 2 cut(s) 57, 358
ScrFI CCNGG 1 cut(s) 363
SetI ASST 3 cut(s) 212, 225, 421
SfaNI GCATC 3 cut(s) 39, 97, 369
SfoI GGCGCC 2 cut(s) 303, 531
SmiMI CAYNNNNRTG 1 cut(s) 354
SphI GCATGC 1 cut(s) 48
Sse9I AATT 3 cut(s) 34, 96, 470
SsiI CCGC 4 cut(s) 308, 326, 542, 584
SspDI GGCGCC 2 cut(s) 301, 529
SspI AATATT 1 cut(s) 79
StyD4I CCNGG 1 cut(s) 361
TaaI ACNGT 2 cut(s) 124, 218
TaiI ACGT 1 cut(s) 212
TaqI TCGA 1 cut(s) 213
TasI AATT 3 cut(s) 34, 96, 470
TatI WGTACW 1 cut(s) 152
TauI GCSGC 1 cut(s) 587
Tru1I TTAA 1 cut(s) 99
Tru9I TTAA 1 cut(s) 99
TscAI CASTG 3 cut(s) 356, 465, 492
TseI GCWGC 3 cut(s) 394, 551, 560
TspDTI ATGAA 1 cut(s) 78
TspRI CASTG 3 cut(s) 356, 465, 492
XceI RCATGY 2 cut(s) 48, 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.