Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0014977

Physical Location & Seq

Genomic Coordinates Forward (+)

29154935 .. 29158535

Gene Structure 3601 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1201200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1098 bp

ATGGAGGGAAAGCACTGGAGATGGAGCTCACAGCCGCGGCCGCGCGATCTGCTAAGAAAAGTCAGAGAATTTACTCGAGCTATGGTTGAAGATCTTAGCAATGAACGGTCTCCGGAGATTATCATCGATCGCTTCAGAAATTACTGCACGAATCCCGATTCAAATTGCTATTGTAGCTTCGATTCACCAAAATGGCGGGAGATTCTCACGCTTCGAAGGGCAAGCCATGTGCGTAGATTAGATGTTTTGCTCAAGGTTCTATTGATTGTTCAGCAACTTCTGCAAGAAAACAGACACGGGTCTAAGAGGGATATCTATTACATGCATCCTTCTATATTTTCAGATCAGTCAGTTGTAGACCGTGCAATCAATGATATATGTATCCTTCTGCAGTGTAGTCGCCACAATTTGAATGTGGTGTCTGTAGGAAAGGGGTTGGTAATGGGCTGGTTAAGGTTTTCAGAAGCTGGAAACGTATTTGATTGCATAAACAGGCCAAACACAGCCTTCTCTGTCCCTATTCATGTTGAAGAGGTCAAAGATATTGTCAGTGTAGCTCGGTGCATACTTGTTGTGGAGAAAGAATCAGTTTTTCAACGATTGGCAGATGACCAGTTTTGCAACAAAAACCATTGCATCGTTATCACTGGACGTGGCTATCCTGATATTCCCACAAGAAGGTTTTTGGGGCTCCTTGTTGATACTTTGCATCTCCCTATCTATTGCTTGGTAGATTGTGATCCATACGGTTTTGACATCCTCTCCATATATCGGTTTGGTTCTATGCAAATGGCCTATGATGCAAAATTTCTACGCGTCCCCGAGGTACTCTGGCTTGGAGCCTTCCCTTCGGACTCAGAGAAGTACAGTCTTCCACAACAGTGCCTCCTTCCCTTGACAGCAGAAGACAAGAGGAAAACGGAGGCAATGCTACTTCGATGCTACTTACAAAGAGAAGCCCCACAGTGGAGATTGGAGCTACAGTTGATGCTGGAAAGAGGAGTGAAGTTTGAGTTAGAAGCGTTGTCTGTGCACGAAATTAACTTTTTGTCGGAGGTGTACATACCATCTAAAATTCAAGGTGGAGTACACATCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000280 GO:0000706 GO:0000724 GO:0000725 GO:0000729 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003916 GO:0003918 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007131 GO:0008094 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0016889 GO:0016894 GO:0017111 GO:0022402 GO:0022414 GO:0022607 GO:0031974 GO:0031981 GO:0033554 GO:0034641 GO:0035825 GO:0042138 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045132 GO:0045143 GO:0046483 GO:0048285 GO:0050896 GO:0051026 GO:0051276 GO:0051321 GO:0051716 GO:0061505 GO:0061982 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko04113 map04113

Pfam Domains

Protein Families

TP6A_N

Protein Analysis

366

Amino Acids

42.48

Weight (kDa)

7.92

Isoelectric Point (pI)

54.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TP6A_N	PF04406	78 - 139	3.3e-21	Type IIB DNA topoisomerase
TOP6A-Spo11_Toprim	PF21180	189 - 359	3.1e-65	Topoisomerase 6 subunit A/Spo11, Toprim domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014977)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G13140
fragaria_vesca	FvH4_3g29421
malus_domestica	MD03G1184600.v1.1 MD03G1184800.v1.1 MD11G1201200.v1.1
prunus_persica	Prupe.4G241700_v2.0.a1 Prupe.5G052600_v2.0.a1
pyrus_communis	pycom11g17370
rosa_chinensis	RchiOBHm_Chr5g0053851
rosa_multiflora	Rmu_sc0001759.1_g000008
rosa_roxburghii	Rroxscaffold_1G00026750
rosa_samantha	Rh5AG353500 Rh5BG365100 Rh5CG390200
rosa_wichuraiana	Rw0G002130 Rw0G009540

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	357
AccII	CGCG	4 cut(s)	37, 43, 45, 816
AccIII	TCCGGA	1 cut(s)	112
AciI	CCGC	4 cut(s)	35, 37, 41, 196
AclWI	GGATC	1 cut(s)	734
AcoI	YGGCCR	1 cut(s)	38
AcsI	RAATTY	3 cut(s)	68, 806, 1074
AcuI	CTGAAG	1 cut(s)	118
AfaI	GTAC	4 cut(s)	828, 866, 1061, 1089
AfiI	CCNNNNNNNGG	3 cut(s)	678, 771, 966
AflIII	ACRYGT	1 cut(s)	814
AgsI	TTSAA	6 cut(s)	89, 162, 412, 530, 596, 1079
AjiI	CACGTC	1 cut(s)	653
AleI	CACNNNNGTG	1 cut(s)	880
AluBI	AGCT	6 cut(s)	27, 80, 177, 467, 557, 979
AluI	AGCT	6 cut(s)	27, 80, 177, 467, 557, 979
Alw21I	GWGCWC	2 cut(s)	29, 1035
Alw26I	GTCTC	1 cut(s)	114
Alw44I	GTGCAC	1 cut(s)	1031
AlwI	GGATC	1 cut(s)	734
AlwNI	CAGNNNCTG	1 cut(s)	467
Ama87I	CYCGRG	2 cut(s)	75, 821
Aor13HI	TCCGGA	1 cut(s)	112
AoxI	GGCC	3 cut(s)	38, 494, 792
ApaLI	GTGCAC	1 cut(s)	1031
ApoI	RAATTY	3 cut(s)	68, 806, 1074
ArsI	GACNNNNNNTTYG	2 cut(s)	531, 563
AspLEI	GCGC	1 cut(s)	45
AsuHPI	GGTGA	1 cut(s)	177
AsuII	TTCGAA	1 cut(s)	214
AvaI	CYCGRG	2 cut(s)	75, 821
BaeGI	GKGCMC	1 cut(s)	1035
BanII	GRGCYC	2 cut(s)	29, 693
BbsI	GAAGAC	2 cut(s)	863, 912
Bbv12I	GWGCWC	2 cut(s)	29, 1035
BccI	CCATC	2 cut(s)	15, 1075
BcgI	CGANNNNNNTGC	2 cut(s)	380, 414
BciVI	GTATCC	1 cut(s)	392
BcoDI	GTCTC	1 cut(s)	114
BfmI	CTRYAG	3 cut(s)	389, 423, 980
BfuI	GTATCC	1 cut(s)	392
BglII	AGATCT	1 cut(s)	91
BisI	GCNGC	3 cut(s)	35, 38, 41
BlsI	GCNGC	3 cut(s)	36, 39, 42
BmeT110I	CYCGRG	2 cut(s)	75, 821
BmgBI	CACGTC	1 cut(s)	653
BmiI	GGNNCC	2 cut(s)	692, 841
BmsI	GCATC	6 cut(s)	334, 645, 718, 790, 929, 978
BoxI	GACNNNNGTC	1 cut(s)	298
BpiI	GAAGAC	2 cut(s)	863, 912
BpmI	CTGGAG	1 cut(s)	37
Bpu14I	TTCGAA	1 cut(s)	214
BpuEI	CTTGAG	1 cut(s)	236
Bsa29I	ATCGAT	1 cut(s)	126
BsaBI	GATNNNNATC	2 cut(s)	122, 738
BsaI	GGTCTC	1 cut(s)	114
BsaJI	CCNNGG	2 cut(s)	35, 822
BsaWI	WCCGGW	1 cut(s)	112
BsaXI	ACNNNNNCTCC	6 cut(s)	746, 776, 870, 900, 968, 998
Bsc4I	CCNNNNNNNGG	3 cut(s)	678, 771, 966
Bse1I	ACTGG	3 cut(s)	20, 613, 652
Bse3DI	GCAATG	3 cut(s)	106, 631, 933
Bse8I	GATNNNNATC	2 cut(s)	122, 738
BseAI	TCCGGA	1 cut(s)	112
BseCI	ATCGAT	1 cut(s)	126
BseDI	CCNNGG	2 cut(s)	35, 822
BseGI	GGATG	2 cut(s)	325, 756
BseJI	GATNNNNATC	2 cut(s)	122, 738
BseLI	CCNNNNNNNGG	3 cut(s)	678, 771, 966
BseMI	GCAATG	3 cut(s)	106, 631, 933
BseMII	CTCAG	1 cut(s)	870
BseNI	ACTGG	3 cut(s)	20, 613, 652
BseRI	GAGGAG	1 cut(s)	1014
BseSI	GKGCMC	1 cut(s)	1035
BseX3I	CGGCCG	1 cut(s)	38
BsgI	GTGCAG	1 cut(s)	130
Bsh1236I	CGCG	4 cut(s)	37, 43, 45, 816
Bsh1285I	CGRYCG	2 cut(s)	41, 130
BshFI	GGCC	3 cut(s)	40, 496, 794
BshVI	ATCGAT	1 cut(s)	126
BsiEI	CGRYCG	2 cut(s)	41, 130
BsiHKAI	GWGCWC	2 cut(s)	29, 1035
BsiHKCI	CYCGRG	2 cut(s)	75, 821
BsiSI	CCGG	1 cut(s)	113
BslFI	GGGAC	2 cut(s)	500, 803
BslI	CCNNNNNNNGG	3 cut(s)	678, 771, 966
BsmAI	GTCTC	1 cut(s)	114
BsmFI	GGGAC	2 cut(s)	500, 803
BsnI	GGCC	3 cut(s)	40, 496, 794
Bso31I	GGTCTC	1 cut(s)	114
BsoBI	CYCGRG	2 cut(s)	75, 821
Bsp119I	TTCGAA	1 cut(s)	214
Bsp1286I	GDGCHC	3 cut(s)	29, 693, 1035
Bsp13I	TCCGGA	1 cut(s)	112
Bsp1407I	TGTACA	1 cut(s)	1059
Bsp143I	GATC	5 cut(s)	46, 91, 127, 343, 739
BspACI	CCGC	4 cut(s)	35, 37, 41, 196
BspANI	GGCC	3 cut(s)	40, 496, 794
BspCNI	CTCAG	1 cut(s)	869
BspDI	ATCGAT	1 cut(s)	126
BspEI	TCCGGA	1 cut(s)	112
BspFNI	CGCG	4 cut(s)	37, 43, 45, 816
BspLI	GGNNCC	2 cut(s)	692, 841
BspMAI	CTGCAG	1 cut(s)	393
BspPI	GGATC	1 cut(s)	734
BspT104I	TTCGAA	1 cut(s)	214
BspTNI	GGTCTC	1 cut(s)	114
BsrDI	GCAATG	3 cut(s)	106, 631, 933
BsrGI	TGTACA	1 cut(s)	1059
BsrI	ACTGG	3 cut(s)	20, 613, 652
BssECI	CCNNGG	2 cut(s)	35, 822
BssMI	GATC	5 cut(s)	46, 91, 127, 343, 739
Bst4CI	ACNGT	7 cut(s)	108, 362, 749, 869, 882, 966, 984
Bst6I	CTCTTC	1 cut(s)	525
BstAPI	GCANNNNNTGC	1 cut(s)	280
BstAUI	TGTACA	1 cut(s)	1059
BstBI	TTCGAA	1 cut(s)	214
BstC8I	GCNNGC	1 cut(s)	223
BstDEI	CTNAG	4 cut(s)	53, 95, 303, 856
BstDSI	CCRYGG	1 cut(s)	35
BstF5I	GGATG	2 cut(s)	325, 756
BstFNI	CGCG	4 cut(s)	37, 43, 45, 816
BstHHI	GCGC	1 cut(s)	45
BstKTI	GATC	5 cut(s)	49, 94, 130, 346, 742
BstMAI	GTCTC	1 cut(s)	114
BstMBI	GATC	5 cut(s)	46, 91, 127, 343, 739
BstMCI	CGRYCG	2 cut(s)	41, 130
BstMWI	GCNNNNNNNGC	5 cut(s)	40, 49, 174, 280, 800
BstNSI	RCATGY	1 cut(s)	325
BstPAI	GACNNNNGTC	1 cut(s)	298
BstSFI	CTRYAG	3 cut(s)	389, 423, 980
BstSLI	GKGCMC	1 cut(s)	1035
BstUI	CGCG	4 cut(s)	37, 43, 45, 816
BstV2I	GAAGAC	2 cut(s)	863, 912
BstX2I	RGATCY	1 cut(s)	91
BstYI	RGATCY	1 cut(s)	91
BstZI	CGGCCG	1 cut(s)	38
Bsu15I	ATCGAT	1 cut(s)	126
BsuI	GTATCC	1 cut(s)	392
BsuRI	GGCC	3 cut(s)	40, 496, 794
BsuTUI	ATCGAT	1 cut(s)	126
BtgI	CCRYGG	1 cut(s)	35
BtrI	CACGTC	1 cut(s)	653
BtsCI	GGATG	2 cut(s)	325, 756
BtsI	GCAGTG	1 cut(s)	398
BtsIMutI	CAGTG	6 cut(s)	13, 398, 556, 645, 887, 971
Cac8I	GCNNGC	1 cut(s)	223
CaiI	CAGNNNCTG	1 cut(s)	467
CciNI	GCGGCCGC	1 cut(s)	38
CfoI	GCGC	1 cut(s)	45
Cfr42I	CCGCGG	1 cut(s)	38
ClaI	ATCGAT	1 cut(s)	126
CseI	GACGC	1 cut(s)	805
Csp6I	GTAC	4 cut(s)	827, 865, 1060, 1088
CviAII	CATG	3 cut(s)	227, 322, 524
CviQI	GTAC	4 cut(s)	827, 865, 1060, 1088
DdeI	CTNAG	4 cut(s)	53, 95, 303, 856
DpnI	GATC	5 cut(s)	48, 93, 129, 345, 741
DpnII	GATC	5 cut(s)	46, 91, 127, 343, 739
EaeI	YGGCCR	1 cut(s)	38
EagI	CGGCCG	1 cut(s)	38
Eam1104I	CTCTTC	1 cut(s)	525
EarI	CTCTTC	1 cut(s)	525
Ecl136II	GAGCTC	1 cut(s)	27
EclXI	CGGCCG	1 cut(s)	38
Eco24I	GRGCYC	2 cut(s)	29, 693
Eco31I	GGTCTC	1 cut(s)	114
Eco32I	GATATC	1 cut(s)	313
Eco52I	CGGCCG	1 cut(s)	38
Eco53kI	GAGCTC	1 cut(s)	27
Eco57I	CTGAAG	1 cut(s)	118
Eco88I	CYCGRG	2 cut(s)	75, 821
EcoICRI	GAGCTC	1 cut(s)	27
EcoRV	GATATC	1 cut(s)	313
EcoT22I	ATGCAT	1 cut(s)	327
EcoT38I	GRGCYC	2 cut(s)	29, 693
FaeI	CATG	3 cut(s)	230, 325, 527
FaqI	GGGAC	2 cut(s)	500, 803
FatI	CATG	3 cut(s)	226, 321, 523
FauI	CCCGC	1 cut(s)	189
FblI	GTMKAC	1 cut(s)	357
Fnu4HI	GCNGC	3 cut(s)	35, 38, 41
FokI	GGATG	2 cut(s)	312, 743
FriOI	GRGCYC	2 cut(s)	29, 693
Fsp4HI	GCNGC	3 cut(s)	35, 38, 41
GlaI	GCGC	1 cut(s)	44
GluI	GCNGC	3 cut(s)	35, 38, 41
GsuI	CTGGAG	1 cut(s)	37
HaeIII	GGCC	3 cut(s)	40, 496, 794
HapII	CCGG	1 cut(s)	113
HgaI	GACGC	1 cut(s)	805
HhaI	GCGC	1 cut(s)	45
Hin1II	CATG	3 cut(s)	230, 325, 527
Hin6I	GCGC	1 cut(s)	43
HinP1I	GCGC	1 cut(s)	43
HinfI	GANTC	6 cut(s)	151, 158, 182, 202, 584, 854
HpaII	CCGG	1 cut(s)	113
HphI	GGTGA	1 cut(s)	177
Hpy166II	GTNNAC	4 cut(s)	358, 1033, 1060, 1090
Hpy188I	TCNGA	7 cut(s)	65, 137, 343, 463, 853, 859, 1054
Hpy188III	TCNNGA	3 cut(s)	113, 155, 662
Hpy8I	GTNNAC	4 cut(s)	358, 1033, 1060, 1090
HpyAV	CCTTC	8 cut(s)	210, 339, 395, 517, 672, 853, 858, 899
HpyCH4III	ACNGT	7 cut(s)	108, 362, 749, 869, 882, 966, 984
HpyCH4IV	ACGT	2 cut(s)	474, 652
HpyF10VI	GCNNNNNNNGC	5 cut(s)	40, 49, 174, 280, 800
HpyF3I	CTNAG	4 cut(s)	53, 95, 303, 856
HpySE526I	ACGT	2 cut(s)	474, 652
Hsp92II	CATG	3 cut(s)	230, 325, 527
HspAI	GCGC	1 cut(s)	43
Kpn2I	TCCGGA	1 cut(s)	112
KspI	CCGCGG	1 cut(s)	38
Kzo9I	GATC	5 cut(s)	46, 91, 127, 343, 739
LmnI	GCTCC	4 cut(s)	24, 696, 839, 976
LpnPI	CCDG	9 cut(s)	126, 433, 453, 478, 626, 633, 675, 817, 977
LweI	GCATC	6 cut(s)	334, 645, 718, 790, 929, 978
MaeII	ACGT	2 cut(s)	474, 652
MalI	GATC	5 cut(s)	48, 93, 129, 345, 741
MboI	GATC	5 cut(s)	46, 91, 127, 343, 739
MboII	GAAGA	4 cut(s)	101, 542, 863, 917
MflI	RGATCY	1 cut(s)	91
MhlI	GDGCHC	3 cut(s)	29, 693, 1035
MluCI	AATT	7 cut(s)	68, 139, 163, 406, 806, 1038, 1074
MluI	ACGCGT	1 cut(s)	814
MlyI	GAGTC	1 cut(s)	848
MmeI	TCCRAC	1 cut(s)	1032
MnlI	CCTC	9 cut(s)	300, 526, 770, 817, 896, 906, 916, 992, 1048
Mph1103I	ATGCAT	1 cut(s)	327
MroI	TCCGGA	1 cut(s)	112
MseI	TTAA	2 cut(s)	452, 1041
MslI	CAYNNNNRTG	2 cut(s)	190, 880
MspA1I	CMGCKG	1 cut(s)	37
MspI	CCGG	1 cut(s)	113
MvnI	CGCG	4 cut(s)	37, 43, 45, 816
MwoI	GCNNNNNNNGC	5 cut(s)	40, 49, 174, 280, 800
NdeII	GATC	5 cut(s)	46, 91, 127, 343, 739
NlaIII	CATG	3 cut(s)	230, 325, 527
NlaIV	GGNNCC	2 cut(s)	692, 841
NotI	GCGGCCGC	1 cut(s)	38
NsiI	ATGCAT	1 cut(s)	327
NspI	RCATGY	1 cut(s)	325
NspV	TTCGAA	1 cut(s)	214
OliI	CACNNNNGTG	1 cut(s)	880
PaeR7I	CTCGAG	1 cut(s)	75
PfeI	GAWTC	5 cut(s)	151, 158, 182, 202, 584
PkrI	GCNGC	3 cut(s)	36, 39, 42
Ple19I	CGATCG	1 cut(s)	130
PleI	GAGTC	1 cut(s)	848
PpsI	GAGTC	1 cut(s)	848
PshAI	GACNNNNGTC	1 cut(s)	298
Psp124BI	GAGCTC	1 cut(s)	29
PspN4I	GGNNCC	2 cut(s)	692, 841
PspXI	VCTCGAGB	1 cut(s)	75
PstI	CTGCAG	1 cut(s)	393
PstNI	CAGNNNCTG	1 cut(s)	467
PsuI	RGATCY	1 cut(s)	91
PvuI	CGATCG	1 cut(s)	130
RsaI	GTAC	4 cut(s)	828, 866, 1061, 1089
RsaNI	GTAC	4 cut(s)	827, 865, 1060, 1088
RseI	CAYNNNNRTG	2 cut(s)	190, 880
SacI	GAGCTC	1 cut(s)	29
SacII	CCGCGG	1 cut(s)	38
SaqAI	TTAA	2 cut(s)	452, 1041
SatI	GCNGC	3 cut(s)	35, 38, 41
Sau3AI	GATC	5 cut(s)	46, 91, 127, 343, 739
SchI	GAGTC	1 cut(s)	848
SduI	GDGCHC	3 cut(s)	29, 693, 1035
SfaNI	GCATC	6 cut(s)	334, 645, 718, 790, 929, 978
SfcI	CTRYAG	3 cut(s)	389, 423, 980
Sfr274I	CTCGAG	1 cut(s)	75
Sfr303I	CCGCGG	1 cut(s)	38
SfuI	TTCGAA	1 cut(s)	214
SgrBI	CCGCGG	1 cut(s)	38
SlaI	CTCGAG	1 cut(s)	75
SmiMI	CAYNNNNRTG	2 cut(s)	190, 880
SmlI	CTYRAG	2 cut(s)	75, 251
SmoI	CTYRAG	2 cut(s)	75, 251
Sse9I	AATT	7 cut(s)	68, 139, 163, 406, 806, 1038, 1074
SsiI	CCGC	4 cut(s)	35, 37, 41, 196
SstI	GAGCTC	1 cut(s)	29
TaaI	ACNGT	7 cut(s)	108, 362, 749, 869, 882, 966, 984
TaiI	ACGT	2 cut(s)	477, 655
TaqI	TCGA	5 cut(s)	76, 126, 180, 214, 937
TasI	AATT	7 cut(s)	68, 139, 163, 406, 806, 1038, 1074
TatI	WGTACW	3 cut(s)	864, 1059, 1087
TauI	GCSGC	3 cut(s)	37, 40, 43
TfiI	GAWTC	5 cut(s)	151, 158, 182, 202, 584
Tru1I	TTAA	2 cut(s)	452, 1041
Tru9I	TTAA	2 cut(s)	452, 1041
TscAI	CASTG	6 cut(s)	20, 398, 556, 652, 887, 971
TspDTI	ATGAA	2 cut(s)	117, 512
TspGWI	ACGGA	1 cut(s)	935
TspRI	CASTG	6 cut(s)	20, 398, 556, 652, 887, 971
VneI	GTGCAC	1 cut(s)	1031
XapI	RAATTY	3 cut(s)	68, 806, 1074
XceI	RCATGY	1 cut(s)	325
XhoI	CTCGAG	1 cut(s)	75
XmiI	GTMKAC	1 cut(s)	357
Zsp2I	ATGCAT	1 cut(s)	327

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.