Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0010474

Physical Location & Seq

Genomic Coordinates Forward (+)

25172170 .. 25177904

Gene Structure 5735 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1185000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1239 bp

ATGTTCATAACAAATTCCATTCCAATACCCAAACAGCCCCTTCTTTCTCTCTCTTTCACTTTCAGTCTCTCTCTCTCCTCAAAACCCTACTCCAATCCCAAACCCCCACACAGCGATCACAACCAAAACCTCTCTAAAACCCTAGCCATGTCCTCGATACTGCTTCGTTCCCGTTCAATCTCCCGGCTATTAAAGGCCAATGGACGCCTATTGAGCTCCGATGTGCCTTCAACGCCTCACAAAGAACCACTCGTCGGAACCCAATCCTTAATCTCCGATACCACATCTCCACCTCCGCCTCCACCGCCGCCACCAAGCGCTCCGGCTCCGAACGCTTCAAGTAGCAAATCCTGGAACTTCCTCAAGTACTCCCTCATTGGCGCGATCACTGGAGCCACTGCTGCTGTTGGTTATGTCTCGTATGCATACTCTTTGGATGAAATTGACGAAAAGACCAAGGCTCTCCGAGCAGCACCTTCAACTAACAAGGTGGCAGATGATGCGTCTGCTCTTGAAAAATTTAAAAGTCGGGTATACTCTTCTACAATTACAGTTCCTGCTAAAGCAGTTGAAGCTTACATTGATGCAAGGAGGACGATTGAAGAACAAATTCGAGGCTATACTGAACCATATGCGGAGAAGCTTCTTCCAGATTTGCATCCCATGGAGAAACATGTTTTTACCCTTGTTCTGGATCTCCAGGAGACATTACTATACTCTTATTGGACGCGAGAAAAGGGCTGGCAGACTATCAAAAGACCTGGAGTTGATGCTTTTTTGGAACATCTTGCTCAGTTTTATGAAATCATCGTGTATTCAGACTATTCAAATATGTATGTAGATCCTGTAATGGAGAGACTGGACACCAAGCATTGTGTACGATATAGGCTGGGAAAGGCTGCCACTAGGTATCAGAATGGAAAACATTATAGGGACCTTTCAAAACTTAACAGAGATCCAAAAAAGATCATCTATCTGAGTGCACACGCACGAGAAAATAGTCTTCAGCCTGAAAATGGTGCCGTAATAAAGCCATATAAGAATGAGTTGGATGACACAGCTCTTGTGGATTTCATACCATTTCTTGAATTTGTTGCCCGCAATCCTCCAGCAGATATTAGGCAAGTACTAGCATCGTATGAAGGGCGTGATATTCCTGCAGAGTTCATTAGGCGATCCAAAGAGCATCAAAAGCGAATGCAAGAATCGAAGCAGCAGGGTCGATTTTGGCGACGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005744 GO:0006464 GO:0006470 GO:0006605 GO:0006626 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006839 GO:0006886 GO:0006996 GO:0007005 GO:0008104 GO:0008150 GO:0008152 GO:0009987 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0017038 GO:0019538 GO:0019866 GO:0030150 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0036211 GO:0042578 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044743 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0065002 GO:0070585 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072655 GO:0098796 GO:0098798 GO:0098800 GO:0140096 GO:1901564 GO:1990542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NIF

Protein Analysis

413

Amino Acids

46.66

Weight (kDa)

9.41

Isoelectric Point (pI)

41.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NIF	PF03031	228 - 367	9e-35	NLI interacting factor-like phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010474)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55900 AT1G55900
fragaria_vesca	FvH4_3g29430 FvH4_3g29430
malus_domestica	MD03G1185000.v1.1 MD11G1201300.v1.1
prunus_persica	Prupe.4G241500_v2.0.a1 Prupe.4G241500_v2.0.a1 Prupe.4G241500_v2.0.a1
pyrus_communis	pycom03g13810
rosa_chinensis	RchiOBHm_Chr5g0053871
rosa_laevigata	RLG00000034907
rosa_roxburghii	Rroxscaffold_1G00026740
rosa_rugosa	Rorug05G0283800 Rorug05G0283900
rosa_samantha	Rh5AG353600 Rh5BG365200 Rh5CG390300 Rh5DG380100
rosa_wichuraiana	Rw0G002120 Rw0G009530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1019
AccI	GTMKAC	1 cut(s)	534
AccII	CGCG	2 cut(s)	383, 730
AciI	CCGC	5 cut(s)	296, 305, 308, 635, 1099
AclWI	GGATC	4 cut(s)	702, 836, 950, 1170
AcsI	RAATTY	4 cut(s)	13, 518, 609, 1088
AcuI	CTGAAG	1 cut(s)	989
AcyI	GRCGYC	1 cut(s)	205
AfaI	GTAC	3 cut(s)	368, 879, 1128
AfeI	AGCGCT	1 cut(s)	319
AfiI	CCNNNNNNNGG	3 cut(s)	254, 691, 1016
AflIII	ACRYGT	1 cut(s)	673
AjnI	CCWGG	3 cut(s)	350, 699, 760
AluBI	AGCT	4 cut(s)	216, 575, 643, 1061
AluI	AGCT	4 cut(s)	216, 575, 643, 1061
Alw21I	GWGCWC	2 cut(s)	218, 985
Alw26I	GTCTC	4 cut(s)	71, 421, 698, 850
Alw44I	GTGCAC	1 cut(s)	981
AlwI	GGATC	4 cut(s)	702, 836, 950, 1170
AlwNI	CAGNNNCTG	1 cut(s)	557
Aor51HI	AGCGCT	1 cut(s)	319
AoxI	GGCC	1 cut(s)	195
ApaLI	GTGCAC	1 cut(s)	981
ApeKI	GCWGC	4 cut(s)	401, 470, 899, 1213
ApoI	RAATTY	4 cut(s)	13, 518, 609, 1088
Asp700I	GAANNNNTTC	1 cut(s)	609
AspLEI	GCGC	2 cut(s)	320, 383
AspS9I	GGNCC	1 cut(s)	934
AsuC2I	CCSGG	1 cut(s)	184
AvaII	GGWCC	1 cut(s)	934
BaeGI	GKGCMC	1 cut(s)	985
BanI	GGYRCC	1 cut(s)	1019
BanII	GRGCYC	1 cut(s)	218
BauI	CACGAG	1 cut(s)	990
BbsI	GAAGAC	1 cut(s)	995
Bbv12I	GWGCWC	2 cut(s)	218, 985
BbvI	GCAGC	4 cut(s)	388, 482, 886, 1225
BceAI	ACGGC	1 cut(s)	1007
BcgI	CGANNNNNNTGC	2 cut(s)	1202, 1236
BciT130I	CCWGG	3 cut(s)	352, 701, 762
BcnI	CCSGG	1 cut(s)	184
BcoDI	GTCTC	4 cut(s)	71, 421, 698, 850
BfaI	CTAG	3 cut(s)	143, 906, 1130
BfmI	CTRYAG	1 cut(s)	1158
BfoI	RGCGCY	1 cut(s)	321
BisI	GCNGC	5 cut(s)	308, 402, 471, 900, 1214
BlsI	GCNGC	5 cut(s)	309, 403, 472, 901, 1215
BmcAI	AGTACT	2 cut(s)	368, 1128
Bme1390I	CCNGG	4 cut(s)	184, 352, 701, 762
Bme18I	GGWCC	1 cut(s)	934
BmgT120I	GGNCC	1 cut(s)	934
BmiI	GGNNCC	5 cut(s)	259, 327, 394, 935, 1021
BmrFI	CCNGG	4 cut(s)	184, 352, 701, 762
BmsI	GCATC	6 cut(s)	490, 574, 667, 760, 1142, 1195
BpiI	GAAGAC	1 cut(s)	995
BpmI	CTGGAG	4 cut(s)	411, 683, 783, 1092
BpuEI	CTTGAG	1 cut(s)	347
BpuMI	CCSGG	1 cut(s)	184
BsaBI	GATNNNNATC	1 cut(s)	657
BsaHI	GRCGYC	1 cut(s)	205
BsaJI	CCNNGG	2 cut(s)	456, 663
BsaXI	ACNNNNNCTCC	2 cut(s)	271, 301
Bsc4I	CCNNNNNNNGG	3 cut(s)	254, 691, 1016
Bse1I	ACTGG	2 cut(s)	394, 864
Bse8I	GATNNNNATC	1 cut(s)	657
BseBI	CCWGG	3 cut(s)	352, 701, 762
BseDI	CCNNGG	2 cut(s)	456, 663
BseGI	GGATG	3 cut(s)	442, 658, 1057
BseJI	GATNNNNATC	1 cut(s)	657
BseLI	CCNNNNNNNGG	3 cut(s)	254, 691, 1016
BseMII	CTCAG	2 cut(s)	806, 968
BseNI	ACTGG	2 cut(s)	394, 864
BseRI	GAGGAG	1 cut(s)	67
BseSI	GKGCMC	1 cut(s)	985
BseXI	GCAGC	4 cut(s)	388, 482, 886, 1225
BseYI	CCCAGC	1 cut(s)	889
Bsh1236I	CGCG	2 cut(s)	383, 730
BshFI	GGCC	1 cut(s)	197
BshNI	GGYRCC	1 cut(s)	1019
BsiHKAI	GWGCWC	2 cut(s)	218, 985
BsiSI	CCGG	2 cut(s)	184, 323
BslFI	GGGAC	1 cut(s)	947
BslI	CCNNNNNNNGG	3 cut(s)	254, 691, 1016
BsmAI	GTCTC	4 cut(s)	71, 421, 698, 850
BsmFI	GGGAC	1 cut(s)	947
BsmI	GAATGC	1 cut(s)	1203
BsnI	GGCC	1 cut(s)	197
Bsp1286I	GDGCHC	2 cut(s)	218, 985
Bsp143I	GATC	7 cut(s)	115, 384, 694, 841, 955, 966, 1175
Bsp19I	CCATGG	1 cut(s)	663
BspACI	CCGC	5 cut(s)	296, 305, 308, 635, 1099
BspANI	GGCC	1 cut(s)	197
BspCNI	CTCAG	2 cut(s)	805, 969
BspFNI	CGCG	2 cut(s)	383, 730
BspLI	GGNNCC	5 cut(s)	259, 327, 394, 935, 1021
BspMAI	CTGCAG	1 cut(s)	1162
BspPI	GGATC	4 cut(s)	702, 836, 950, 1170
BspT107I	GGYRCC	1 cut(s)	1019
BsrI	ACTGG	2 cut(s)	394, 864
BssECI	CCNNGG	2 cut(s)	456, 663
BssMI	GATC	7 cut(s)	115, 384, 694, 841, 955, 966, 1175
BssNAI	GTATAC	1 cut(s)	535
BssNI	GRCGYC	1 cut(s)	205
BssSI	CACGAG	1 cut(s)	990
BssT1I	CCWWGG	2 cut(s)	456, 663
Bst1107I	GTATAC	1 cut(s)	535
Bst2BI	CACGAG	1 cut(s)	990
Bst2UI	CCWGG	3 cut(s)	352, 701, 762
Bst4CI	ACNGT	1 cut(s)	553
Bst6I	CTCTTC	1 cut(s)	544
BstACI	GRCGYC	1 cut(s)	205
BstAPI	GCANNNNNTGC	1 cut(s)	500
BstC8I	GCNNGC	2 cut(s)	743, 1099
BstDEI	CTNAG	2 cut(s)	792, 977
BstDSI	CCRYGG	1 cut(s)	663
BstF5I	GGATG	3 cut(s)	442, 658, 1057
BstFNI	CGCG	2 cut(s)	383, 730
BstH2I	RGCGCY	1 cut(s)	321
BstHHI	GCGC	2 cut(s)	320, 383
BstKTI	GATC	7 cut(s)	118, 387, 697, 844, 958, 969, 1178
BstMAI	GTCTC	4 cut(s)	71, 421, 698, 850
BstMBI	GATC	7 cut(s)	115, 384, 694, 841, 955, 966, 1175
BstMWI	GCNNNNNNNGC	9 cut(s)	213, 232, 304, 332, 401, 467, 500, 572, 1192
BstNI	CCWGG	3 cut(s)	352, 701, 762
BstNSI	RCATGY	1 cut(s)	677
BstSCI	CCNGG	4 cut(s)	182, 350, 699, 760
BstSFI	CTRYAG	1 cut(s)	1158
BstSLI	GKGCMC	1 cut(s)	985
BstUI	CGCG	2 cut(s)	383, 730
BstV1I	GCAGC	4 cut(s)	388, 482, 886, 1225
BstV2I	GAAGAC	1 cut(s)	995
BstX2I	RGATCY	3 cut(s)	694, 841, 955
BstYI	RGATCY	3 cut(s)	694, 841, 955
BstZ17I	GTATAC	1 cut(s)	535
BsuRI	GGCC	1 cut(s)	197
BtgI	CCRYGG	1 cut(s)	663
BtsCI	GGATG	3 cut(s)	442, 658, 1057
BtsI	GCAGTG	1 cut(s)	396
BtsIMutI	CAGTG	2 cut(s)	387, 396
Cac8I	GCNNGC	2 cut(s)	743, 1099
CaiI	CAGNNNCTG	1 cut(s)	557
CfoI	GCGC	2 cut(s)	320, 383
Cfr13I	GGNCC	1 cut(s)	934
CseI	GACGC	3 cut(s)	213, 492, 736
Csp6I	GTAC	3 cut(s)	367, 878, 1127
CviAII	CATG	3 cut(s)	148, 664, 674
CviQI	GTAC	3 cut(s)	367, 878, 1127
DdeI	CTNAG	2 cut(s)	792, 977
DpnI	GATC	7 cut(s)	117, 386, 696, 843, 957, 968, 1177
DpnII	GATC	7 cut(s)	115, 384, 694, 841, 955, 966, 1175
DraI	TTTAAA	1 cut(s)	523
Eam1104I	CTCTTC	1 cut(s)	544
EarI	CTCTTC	1 cut(s)	544
EciI	GGCGGA	1 cut(s)	285
Ecl136II	GAGCTC	1 cut(s)	216
Eco130I	CCWWGG	2 cut(s)	456, 663
Eco24I	GRGCYC	1 cut(s)	218
Eco47I	GGWCC	1 cut(s)	934
Eco47III	AGCGCT	1 cut(s)	319
Eco53kI	GAGCTC	1 cut(s)	216
Eco57I	CTGAAG	1 cut(s)	989
EcoICRI	GAGCTC	1 cut(s)	216
EcoO109I	RGGNCCY	1 cut(s)	934
EcoRII	CCWGG	3 cut(s)	350, 699, 760
EcoT14I	CCWWGG	2 cut(s)	456, 663
EcoT22I	ATGCAT	1 cut(s)	427
EcoT38I	GRGCYC	1 cut(s)	218
ErhI	CCWWGG	2 cut(s)	456, 663
FaeI	CATG	3 cut(s)	151, 667, 677
FaqI	GGGAC	1 cut(s)	947
FatI	CATG	3 cut(s)	147, 663, 673
FauI	CCCGC	1 cut(s)	1106
FauNDI	CATATG	1 cut(s)	631
FblI	GTMKAC	1 cut(s)	534
Fnu4HI	GCNGC	5 cut(s)	308, 402, 471, 900, 1214
FokI	GGATG	3 cut(s)	449, 645, 1064
FriOI	GRGCYC	1 cut(s)	218
Fsp4HI	GCNGC	5 cut(s)	308, 402, 471, 900, 1214
FspBI	CTAG	3 cut(s)	143, 906, 1130
GlaI	GCGC	2 cut(s)	319, 382
GluI	GCNGC	5 cut(s)	308, 402, 471, 900, 1214
GsaI	CCCAGC	1 cut(s)	893
GsuI	CTGGAG	4 cut(s)	411, 683, 783, 1092
HaeII	RGCGCY	1 cut(s)	321
HaeIII	GGCC	1 cut(s)	197
HapII	CCGG	2 cut(s)	184, 323
HgaI	GACGC	3 cut(s)	213, 492, 736
HhaI	GCGC	2 cut(s)	320, 383
Hin1I	GRCGYC	1 cut(s)	205
Hin1II	CATG	3 cut(s)	151, 667, 677
Hin6I	GCGC	2 cut(s)	318, 381
HinP1I	GCGC	2 cut(s)	318, 381
HindIII	AAGCTT	2 cut(s)	573, 641
HinfI	GANTC	1 cut(s)	1205
HpaII	CCGG	2 cut(s)	184, 323
Hpy166II	GTNNAC	3 cut(s)	535, 878, 983
Hpy188I	TCNGA	8 cut(s)	220, 257, 277, 330, 467, 820, 915, 978
Hpy188III	TCNNGA	4 cut(s)	512, 650, 692, 1085
Hpy8I	GTNNAC	3 cut(s)	535, 878, 983
Hpy99I	CGWCG	2 cut(s)	257, 1236
HpyAV	CCTTC	4 cut(s)	50, 237, 486, 1136
HpyCH4III	ACNGT	1 cut(s)	553
HpyCH4IV	ACGT	1 cut(s)	1234
HpyCH4V	TGCA	6 cut(s)	425, 587, 658, 983, 1160, 1201
HpyF10VI	GCNNNNNNNGC	9 cut(s)	213, 232, 304, 332, 401, 467, 500, 572, 1192
HpyF3I	CTNAG	2 cut(s)	792, 977
HpySE526I	ACGT	1 cut(s)	1234
Hsp92I	GRCGYC	1 cut(s)	205
Hsp92II	CATG	3 cut(s)	151, 667, 677
HspAI	GCGC	2 cut(s)	318, 381
Kzo9I	GATC	7 cut(s)	115, 384, 694, 841, 955, 966, 1175
LmnI	GCTCC	4 cut(s)	221, 325, 331, 392
Lsp1109I	GCAGC	4 cut(s)	388, 482, 886, 1225
LweI	GCATC	6 cut(s)	490, 574, 667, 760, 1142, 1195
MaeI	CTAG	3 cut(s)	143, 906, 1130
MaeII	ACGT	1 cut(s)	1234
MalI	GATC	7 cut(s)	117, 386, 696, 843, 957, 968, 1177
MboI	GATC	7 cut(s)	115, 384, 694, 841, 955, 966, 1175
MboII	GAAGA	4 cut(s)	531, 614, 638, 995
MflI	RGATCY	3 cut(s)	694, 841, 955
MhlI	GDGCHC	2 cut(s)	218, 985
MluCI	AATT	6 cut(s)	13, 441, 518, 546, 609, 1088
MmeI	TCCRAC	2 cut(s)	235, 1029
Mph1103I	ATGCAT	1 cut(s)	427
MroXI	GAANNNNTTC	1 cut(s)	609
MseI	TTAA	4 cut(s)	191, 269, 522, 948
MspI	CCGG	2 cut(s)	184, 323
MspR9I	CCNGG	4 cut(s)	184, 352, 701, 762
Mva1269I	GAATGC	1 cut(s)	1203
MvaI	CCWGG	3 cut(s)	352, 701, 762
MvnI	CGCG	2 cut(s)	383, 730
MwoI	GCNNNNNNNGC	9 cut(s)	213, 232, 304, 332, 401, 467, 500, 572, 1192
NciI	CCSGG	1 cut(s)	184
NcoI	CCATGG	1 cut(s)	663
NdeI	CATATG	1 cut(s)	631
NdeII	GATC	7 cut(s)	115, 384, 694, 841, 955, 966, 1175
NlaIII	CATG	3 cut(s)	151, 667, 677
NlaIV	GGNNCC	5 cut(s)	259, 327, 394, 935, 1021
NsiI	ATGCAT	1 cut(s)	427
NspI	RCATGY	1 cut(s)	677
PciI	ACATGT	1 cut(s)	673
PcsI	WCGNNNNNNNCGW	1 cut(s)	1228
PctI	GAATGC	1 cut(s)	1203
PdmI	GAANNNNTTC	1 cut(s)	609
PfeI	GAWTC	1 cut(s)	1205
PfoI	TCCNGGA	2 cut(s)	350, 699
PkrI	GCNGC	5 cut(s)	309, 403, 472, 901, 1215
PpuMI	RGGWCCY	1 cut(s)	934
PscI	ACATGT	1 cut(s)	673
Psp124BI	GAGCTC	1 cut(s)	218
Psp5II	RGGWCCY	1 cut(s)	934
Psp6I	CCWGG	3 cut(s)	350, 699, 760
PspFI	CCCAGC	1 cut(s)	889
PspGI	CCWGG	3 cut(s)	350, 699, 760
PspN4I	GGNNCC	5 cut(s)	259, 327, 394, 935, 1021
PspPI	GGNCC	1 cut(s)	934
PspPPI	RGGWCCY	1 cut(s)	934
PstI	CTGCAG	1 cut(s)	1162
PstNI	CAGNNNCTG	1 cut(s)	557
PsuI	RGATCY	3 cut(s)	694, 841, 955
RsaI	GTAC	3 cut(s)	368, 879, 1128
RsaNI	GTAC	3 cut(s)	367, 878, 1127
SacI	GAGCTC	1 cut(s)	218
SaqAI	TTAA	4 cut(s)	191, 269, 522, 948
SatI	GCNGC	5 cut(s)	308, 402, 471, 900, 1214
Sau3AI	GATC	7 cut(s)	115, 384, 694, 841, 955, 966, 1175
Sau96I	GGNCC	1 cut(s)	934
ScaI	AGTACT	2 cut(s)	368, 1128
ScrFI	CCNGG	4 cut(s)	184, 352, 701, 762
SduI	GDGCHC	2 cut(s)	218, 985
SfaNI	GCATC	6 cut(s)	490, 574, 667, 760, 1142, 1195
SfcI	CTRYAG	1 cut(s)	1158
SinI	GGWCC	1 cut(s)	934
SmlI	CTYRAG	1 cut(s)	362
SmoI	CTYRAG	1 cut(s)	362
Sse9I	AATT	6 cut(s)	13, 441, 518, 546, 609, 1088
SsiI	CCGC	5 cut(s)	296, 305, 308, 635, 1099
SspMI	CTAG	3 cut(s)	143, 906, 1130
SstI	GAGCTC	1 cut(s)	218
StyD4I	CCNGG	4 cut(s)	182, 350, 699, 760
StyI	CCWWGG	2 cut(s)	456, 663
TaaI	ACNGT	1 cut(s)	553
TaiI	ACGT	1 cut(s)	1237
TaqI	TCGA	4 cut(s)	155, 613, 1208, 1222
TasI	AATT	6 cut(s)	13, 441, 518, 546, 609, 1088
TatI	WGTACW	2 cut(s)	366, 1126
TauI	GCSGC	1 cut(s)	310
TfiI	GAWTC	1 cut(s)	1205
Tru1I	TTAA	4 cut(s)	191, 269, 522, 948
Tru9I	TTAA	4 cut(s)	191, 269, 522, 948
TscAI	CASTG	2 cut(s)	394, 403
TseI	GCWGC	4 cut(s)	401, 470, 899, 1213
TspDTI	ATGAA	5 cut(s)	453, 816, 1063, 1155, 1156
TspRI	CASTG	2 cut(s)	394, 403
VneI	GTGCAC	1 cut(s)	981
VpaK11BI	GGWCC	1 cut(s)	934
XapI	RAATTY	4 cut(s)	13, 518, 609, 1088
XceI	RCATGY	1 cut(s)	677
XmiI	GTMKAC	1 cut(s)	534
XmnI	GAANNNNTTC	1 cut(s)	609
XspI	CTAG	3 cut(s)	143, 906, 1130
ZrmI	AGTACT	2 cut(s)	368, 1128
Zsp2I	ATGCAT	1 cut(s)	427

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.