Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0010474

Physical Location & Seq

Genomic Coordinates Forward (+)

56854490 .. 56859869

Gene Structure 5380 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000034907

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1092 bp

ATGTCTTCAACACTACTTCGCTCCCGTTTAGCCTCTCTCGTATCGAAGCGCGGTCGCCGTCTATTGAGCTCCGACGTCGCTTCGAACCCTCCTAAGGACCCATTCGTTGCCGCCGAAACCCTCGCTTCCAATCGCGCAACTCCTCCACCGCCACCGCCACCGCCAAGCGCCGCCGCTGCGAGCGCGTCAACTAGAAAGCCCTGGAGCTTTCTCAAGTACTCCCTCGTTGGCGCGCTCACCGGCGCCACCGCCTCCGTTGCTTATGTCTCCTATGCTTACTCGATTGATGAAATAGCTGAAAAGACAAAGGCTCTGCGAGAAGCGCATGCAAGTATCGAAATTCCAGATGATGCGTCTACTCTTGACAAATTCAAAACTATGCTCTACTCTTCTGCAATTATAGTGCCTGCTAAAGCAGCTGAAGCTTACCTTGATACTAGGAGGGCAATTGAAGAACAAGTTCGAGGTTATACTGAACCATATGCGGAAAAGCTTCTTCCAGATTTGCAACCCTGGGAGAGAAATGTTTATACACTTGTTCTGGATCTACAGGAGACATTACTTTACTCTTATTGGACGAGGGAAAAGGGCTGGCAGACGATCAAAAGACCTGGAGTTGATGCTTTCTTAGAGCATCTTGCTCAGTATTATGAAATCATTGTATTTTCGGACTATTCAAATATGTATGTAGATCCTGTAATGGAGAGATTGGACCCAAAACATTGTGTAAGATTCAGGCTAGGAAAGGCTGCCACCAAGTATCAAGATGGAGTGCATTATAGGGACCTTTCAAAGCTAAATAGAGATCCAAGAAAGATCATATATCTGAGTGCCCATGCACGAGAAAATAGCCTACAGCCTGAAAATGGTGCCGTAATAAAGCCATATAAGTCTGAGCTGGATGATACAGCTCTTGTAGATTTCATCCCATTTCTTGAATTTGTCGCCCGTAACCCTCCAGCAGATATCAGACAAGTATTAGCATCGTATGAAGGACATGAAATTCCAGCTGAGTTCATTAGGCGTACTAAGGAGCATCAGAGGAAAATGCAAGAACAGAAGCAGCATGGTCCTCGACTCTGGAGGCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005744 GO:0006464 GO:0006470 GO:0006605 GO:0006626 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006839 GO:0006886 GO:0006996 GO:0007005 GO:0008104 GO:0008150 GO:0008152 GO:0009987 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0017038 GO:0019538 GO:0019866 GO:0030150 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0036211 GO:0042578 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044743 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0065002 GO:0070585 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072655 GO:0098796 GO:0098798 GO:0098800 GO:0140096 GO:1901564 GO:1990542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NIF

Protein Analysis

364

Amino Acids

41.27

Weight (kDa)

9.07

Isoelectric Point (pI)

40.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NIF	PF03031	177 - 317	1.9e-35	NLI interacting factor-like phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010474)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55900 AT1G55900
fragaria_vesca	FvH4_3g29430 FvH4_3g29430
malus_domestica	MD03G1185000.v1.1 MD11G1201300.v1.1
prunus_persica	Prupe.4G241500_v2.0.a1 Prupe.4G241500_v2.0.a1 Prupe.4G241500_v2.0.a1
pyrus_communis	pycom03g13810
rosa_chinensis	RchiOBHm_Chr5g0053871
rosa_laevigata	RLG00000034907
rosa_roxburghii	Rroxscaffold_1G00026740
rosa_rugosa	Rorug05G0283800 Rorug05G0283900
rosa_samantha	Rh5AG353600 Rh5BG365200 Rh5CG390300 Rh5DG380100
rosa_wichuraiana	Rw0G002120 Rw0G009530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	78
AccB1I	GGYRCC	2 cut(s)	242, 869
AccI	GTMKAC	1 cut(s)	356
AccII	CGCG	4 cut(s)	51, 135, 185, 233
AciI	CCGC	9 cut(s)	51, 111, 149, 155, 161, 171, 174, 249, 485
AclWI	GGATC	3 cut(s)	552, 686, 800
AcsI	RAATTY	4 cut(s)	339, 368, 938, 1002
AcuI	CTGAAG	1 cut(s)	441
AcyI	GRCGYC	2 cut(s)	75, 243
AfaI	GTAC	2 cut(s)	218, 1027
AfiI	CCNNNNNNNGG	2 cut(s)	94, 866
AgsI	TTSAA	6 cut(s)	9, 373, 452, 678, 792, 938
AjnI	CCWGG	3 cut(s)	200, 512, 610
Alw21I	GWGCWC	1 cut(s)	71
Alw26I	GTCTC	2 cut(s)	271, 548
AlwI	GGATC	3 cut(s)	552, 686, 800
ApeKI	GCWGC	4 cut(s)	176, 416, 749, 1063
ApoI	RAATTY	4 cut(s)	339, 368, 938, 1002
Asp700I	GAANNNNTTC	2 cut(s)	459, 492
AspLEI	GCGC	8 cut(s)	51, 137, 170, 185, 233, 235, 245, 325
AspS9I	GGNCC	4 cut(s)	97, 712, 784, 1070
AsuHPI	GGTGA	1 cut(s)	229
AsuII	TTCGAA	1 cut(s)	83
AvaII	GGWCC	4 cut(s)	97, 712, 784, 1070
AxyI	CCTNAGG	1 cut(s)	93
BaeGI	GKGCMC	1 cut(s)	835
BanI	GGYRCC	2 cut(s)	242, 869
BanII	GRGCYC	1 cut(s)	71
BauI	CACGAG	1 cut(s)	840
Bbv12I	GWGCWC	1 cut(s)	71
BbvI	GCAGC	4 cut(s)	163, 428, 736, 1075
BccI	CCATC	1 cut(s)	761
BceAI	ACGGC	2 cut(s)	42, 857
BcgI	CGANNNNNNTGC	2 cut(s)	1055, 1089
BciT130I	CCWGG	3 cut(s)	202, 514, 612
BcoDI	GTCTC	2 cut(s)	271, 548
BfaI	CTAG	3 cut(s)	192, 438, 740
BfmI	CTRYAG	2 cut(s)	548, 854
BfoI	RGCGCY	2 cut(s)	171, 246
BisI	GCNGC	7 cut(s)	111, 171, 174, 177, 417, 750, 1064
BlsI	GCNGC	7 cut(s)	112, 172, 175, 178, 418, 751, 1065
BmcAI	AGTACT	1 cut(s)	218
Bme1390I	CCNGG	3 cut(s)	202, 514, 612
Bme18I	GGWCC	4 cut(s)	97, 712, 784, 1070
BmgT120I	GGNCC	4 cut(s)	97, 712, 784, 1070
BmiI	GGNNCC	5 cut(s)	99, 244, 714, 785, 871
BmrFI	CCNGG	3 cut(s)	202, 514, 612
BmsI	GCATC	5 cut(s)	340, 610, 643, 992, 1045
BpmI	CTGGAG	3 cut(s)	223, 633, 942
Bpu14I	TTCGAA	1 cut(s)	83
BpuEI	CTTGAG	1 cut(s)	197
BsaHI	GRCGYC	2 cut(s)	75, 243
BsaJI	CCNNGG	3 cut(s)	200, 512, 513
Bsc4I	CCNNNNNNNGG	2 cut(s)	94, 866
Bse118I	RCCGGY	1 cut(s)	239
Bse21I	CCTNAGG	1 cut(s)	93
BseBI	CCWGG	3 cut(s)	202, 514, 612
BseDI	CCNNGG	3 cut(s)	200, 512, 513
BseGI	GGATG	2 cut(s)	907, 924
BseLI	CCNNNNNNNGG	2 cut(s)	94, 866
BseMII	CTCAG	4 cut(s)	656, 818, 885, 1002
BsePI	GCGCGC	1 cut(s)	231
BseRI	GAGGAG	1 cut(s)	132
BseSI	GKGCMC	1 cut(s)	835
BseXI	GCAGC	4 cut(s)	163, 428, 736, 1075
Bsh1236I	CGCG	4 cut(s)	51, 135, 185, 233
Bsh1285I	CGRYCG	1 cut(s)	55
BshNI	GGYRCC	2 cut(s)	242, 869
BsiEI	CGRYCG	1 cut(s)	55
BsiHKAI	GWGCWC	1 cut(s)	71
BsiSI	CCGG	1 cut(s)	240
BslFI	GGGAC	1 cut(s)	797
BslI	CCNNNNNNNGG	2 cut(s)	94, 866
BsmAI	GTCTC	2 cut(s)	271, 548
BsmFI	GGGAC	1 cut(s)	797
Bsp119I	TTCGAA	1 cut(s)	83
Bsp1286I	GDGCHC	2 cut(s)	71, 835
Bsp143I	GATC	5 cut(s)	544, 600, 691, 805, 816
BspACI	CCGC	9 cut(s)	51, 111, 149, 155, 161, 171, 174, 249, 485
BspCNI	CTCAG	4 cut(s)	655, 819, 886, 1003
BspFNI	CGCG	4 cut(s)	51, 135, 185, 233
BspLI	GGNNCC	5 cut(s)	99, 244, 714, 785, 871
BspPI	GGATC	3 cut(s)	552, 686, 800
BspT104I	TTCGAA	1 cut(s)	83
BspT107I	GGYRCC	2 cut(s)	242, 869
BsrFI	RCCGGY	1 cut(s)	239
BssAI	RCCGGY	1 cut(s)	239
BssECI	CCNNGG	3 cut(s)	200, 512, 513
BssHII	GCGCGC	1 cut(s)	231
BssMI	GATC	5 cut(s)	544, 600, 691, 805, 816
BssNI	GRCGYC	2 cut(s)	75, 243
BssSI	CACGAG	1 cut(s)	840
Bst2BI	CACGAG	1 cut(s)	840
Bst2UI	CCWGG	3 cut(s)	202, 514, 612
Bst6I	CTCTTC	1 cut(s)	394
BstACI	GRCGYC	2 cut(s)	75, 243
BstBI	TTCGAA	1 cut(s)	83
BstC8I	GCNNGC	5 cut(s)	181, 233, 327, 408, 593
BstDEI	CTNAG	7 cut(s)	93, 628, 642, 827, 894, 1011, 1029
BstF5I	GGATG	2 cut(s)	907, 924
BstFNI	CGCG	4 cut(s)	51, 135, 185, 233
BstH2I	RGCGCY	2 cut(s)	171, 246
BstHHI	GCGC	8 cut(s)	51, 137, 170, 185, 233, 235, 245, 325
BstKTI	GATC	5 cut(s)	547, 603, 694, 808, 819
BstMAI	GTCTC	2 cut(s)	271, 548
BstMBI	GATC	5 cut(s)	544, 600, 691, 805, 816
BstMCI	CGRYCG	1 cut(s)	55
BstMWI	GCNNNNNNNGC	6 cut(s)	176, 182, 257, 322, 416, 422
BstNI	CCWGG	3 cut(s)	202, 514, 612
BstNSI	RCATGY	1 cut(s)	329
BstSCI	CCNGG	3 cut(s)	200, 512, 610
BstSFI	CTRYAG	2 cut(s)	548, 854
BstSLI	GKGCMC	1 cut(s)	835
BstUI	CGCG	4 cut(s)	51, 135, 185, 233
BstV1I	GCAGC	4 cut(s)	163, 428, 736, 1075
BstX2I	RGATCY	3 cut(s)	544, 691, 805
BstYI	RGATCY	3 cut(s)	544, 691, 805
Bsu36I	CCTNAGG	1 cut(s)	93
BtsCI	GGATG	2 cut(s)	907, 924
Cac8I	GCNNGC	5 cut(s)	181, 233, 327, 408, 593
CfoI	GCGC	8 cut(s)	51, 137, 170, 185, 233, 235, 245, 325
Cfr10I	RCCGGY	1 cut(s)	239
Cfr13I	GGNCC	4 cut(s)	97, 712, 784, 1070
CseI	GACGC	2 cut(s)	174, 342
Csp6I	GTAC	2 cut(s)	217, 1026
CviAII	CATG	4 cut(s)	326, 836, 998, 1067
CviQI	GTAC	2 cut(s)	217, 1026
DdeI	CTNAG	7 cut(s)	93, 628, 642, 827, 894, 1011, 1029
DinI	GGCGCC	1 cut(s)	244
DpnI	GATC	5 cut(s)	546, 602, 693, 807, 818
DpnII	GATC	5 cut(s)	544, 600, 691, 805, 816
Eam1104I	CTCTTC	1 cut(s)	394
EarI	CTCTTC	1 cut(s)	394
Ecl136II	GAGCTC	1 cut(s)	69
Eco24I	GRGCYC	1 cut(s)	71
Eco32I	GATATC	1 cut(s)	967
Eco47I	GGWCC	4 cut(s)	97, 712, 784, 1070
Eco53kI	GAGCTC	1 cut(s)	69
Eco57I	CTGAAG	1 cut(s)	441
Eco81I	CCTNAGG	1 cut(s)	93
EcoICRI	GAGCTC	1 cut(s)	69
EcoO109I	RGGNCCY	2 cut(s)	97, 784
EcoRII	CCWGG	3 cut(s)	200, 512, 610
EcoRV	GATATC	1 cut(s)	967
EcoT38I	GRGCYC	1 cut(s)	71
EgeI	GGCGCC	1 cut(s)	244
EheI	GGCGCC	1 cut(s)	244
FaeI	CATG	4 cut(s)	329, 839, 1001, 1070
FalI	AAGNNNNNCTT	2 cut(s)	414, 446
FaqI	GGGAC	1 cut(s)	797
FatI	CATG	4 cut(s)	325, 835, 997, 1066
FauNDI	CATATG	1 cut(s)	481
FblI	GTMKAC	1 cut(s)	356
Fnu4HI	GCNGC	7 cut(s)	111, 171, 174, 177, 417, 750, 1064
FokI	GGATG	2 cut(s)	911, 914
FriOI	GRGCYC	1 cut(s)	71
Fsp4HI	GCNGC	7 cut(s)	111, 171, 174, 177, 417, 750, 1064
FspBI	CTAG	3 cut(s)	192, 438, 740
GlaI	GCGC	8 cut(s)	50, 136, 169, 184, 232, 234, 244, 324
GluI	GCNGC	7 cut(s)	111, 171, 174, 177, 417, 750, 1064
GsuI	CTGGAG	3 cut(s)	223, 633, 942
HaeII	RGCGCY	2 cut(s)	171, 246
HapII	CCGG	1 cut(s)	240
HgaI	GACGC	2 cut(s)	174, 342
HhaI	GCGC	8 cut(s)	51, 137, 170, 185, 233, 235, 245, 325
Hin1I	GRCGYC	2 cut(s)	75, 243
Hin1II	CATG	4 cut(s)	329, 839, 1001, 1070
Hin6I	GCGC	8 cut(s)	49, 135, 168, 183, 231, 233, 243, 323
HinP1I	GCGC	8 cut(s)	49, 135, 168, 183, 231, 233, 243, 323
HincII	GTYRAC	1 cut(s)	189
HindII	GTYRAC	1 cut(s)	189
HindIII	AAGCTT	2 cut(s)	423, 491
HinfI	GANTC	2 cut(s)	732, 1077
HpaII	CCGG	1 cut(s)	240
HphI	GGTGA	1 cut(s)	229
Hpy166II	GTNNAC	2 cut(s)	189, 357
Hpy188I	TCNGA	6 cut(s)	73, 670, 828, 895, 971, 1041
Hpy188III	TCNNGA	7 cut(s)	344, 362, 500, 542, 764, 935, 1081
Hpy8I	GTNNAC	2 cut(s)	189, 357
Hpy99I	CGWCG	2 cut(s)	77, 80
HpyAV	CCTTC	1 cut(s)	986
HpyCH4IV	ACGT	1 cut(s)	75
HpyCH4V	TGCA	6 cut(s)	329, 395, 508, 775, 839, 1051
HpyF10VI	GCNNNNNNNGC	6 cut(s)	176, 182, 257, 322, 416, 422
HpyF3I	CTNAG	7 cut(s)	93, 628, 642, 827, 894, 1011, 1029
HpySE526I	ACGT	1 cut(s)	75
Hsp92I	GRCGYC	2 cut(s)	75, 243
Hsp92II	CATG	4 cut(s)	329, 839, 1001, 1070
HspAI	GCGC	8 cut(s)	49, 135, 168, 183, 231, 233, 243, 323
KasI	GGCGCC	1 cut(s)	242
Kzo9I	GATC	5 cut(s)	544, 600, 691, 805, 816
LmnI	GCTCC	4 cut(s)	26, 74, 204, 1033
Lsp1109I	GCAGC	4 cut(s)	163, 428, 736, 1075
LweI	GCATC	5 cut(s)	340, 610, 643, 992, 1045
MaeI	CTAG	3 cut(s)	192, 438, 740
MaeII	ACGT	1 cut(s)	75
MaeIII	GTNAC	1 cut(s)	950
MalI	GATC	5 cut(s)	546, 602, 693, 807, 818
MboI	GATC	5 cut(s)	544, 600, 691, 805, 816
MboII	GAAGA	3 cut(s)	381, 464, 488
MfeI	CAATTG	1 cut(s)	447
MflI	RGATCY	3 cut(s)	544, 691, 805
MhlI	GDGCHC	2 cut(s)	71, 835
MluCI	AATT	6 cut(s)	339, 368, 396, 447, 938, 1002
Mly113I	GGCGCC	1 cut(s)	243
MlyI	GAGTC	1 cut(s)	1071
MmeI	TCCRAC	1 cut(s)	96
MroXI	GAANNNNTTC	2 cut(s)	459, 492
MspA1I	CMGCKG	3 cut(s)	176, 419, 1010
MspI	CCGG	1 cut(s)	240
MspR9I	CCNGG	3 cut(s)	202, 514, 612
MunI	CAATTG	1 cut(s)	447
MvaI	CCWGG	3 cut(s)	202, 514, 612
MvnI	CGCG	4 cut(s)	51, 135, 185, 233
MwoI	GCNNNNNNNGC	6 cut(s)	176, 182, 257, 322, 416, 422
NarI	GGCGCC	1 cut(s)	243
NdeI	CATATG	1 cut(s)	481
NdeII	GATC	5 cut(s)	544, 600, 691, 805, 816
NlaIII	CATG	4 cut(s)	329, 839, 1001, 1070
NlaIV	GGNNCC	5 cut(s)	99, 244, 714, 785, 871
NspI	RCATGY	1 cut(s)	329
NspV	TTCGAA	1 cut(s)	83
PaeI	GCATGC	1 cut(s)	329
PasI	CCCWGGG	1 cut(s)	513
PauI	GCGCGC	1 cut(s)	231
PcsI	WCGNNNNNNNCGW	1 cut(s)	111
PdmI	GAANNNNTTC	2 cut(s)	459, 492
PfeI	GAWTC	1 cut(s)	732
PkrI	GCNGC	7 cut(s)	112, 172, 175, 178, 418, 751, 1065
PleI	GAGTC	1 cut(s)	1071
PluTI	GGCGCC	1 cut(s)	246
PpsI	GAGTC	1 cut(s)	1071
PpuMI	RGGWCCY	2 cut(s)	97, 784
Psp124BI	GAGCTC	1 cut(s)	71
Psp5II	RGGWCCY	2 cut(s)	97, 784
Psp6I	CCWGG	3 cut(s)	200, 512, 610
PspGI	CCWGG	3 cut(s)	200, 512, 610
PspN4I	GGNNCC	5 cut(s)	99, 244, 714, 785, 871
PspPI	GGNCC	4 cut(s)	97, 712, 784, 1070
PspPPI	RGGWCCY	2 cut(s)	97, 784
PsuI	RGATCY	3 cut(s)	544, 691, 805
PteI	GCGCGC	1 cut(s)	231
PvuII	CAGCTG	2 cut(s)	419, 1010
RsaI	GTAC	2 cut(s)	218, 1027
RsaNI	GTAC	2 cut(s)	217, 1026
SacI	GAGCTC	1 cut(s)	71
SatI	GCNGC	7 cut(s)	111, 171, 174, 177, 417, 750, 1064
Sau3AI	GATC	5 cut(s)	544, 600, 691, 805, 816
Sau96I	GGNCC	4 cut(s)	97, 712, 784, 1070
ScaI	AGTACT	1 cut(s)	218
SchI	GAGTC	1 cut(s)	1071
ScrFI	CCNGG	3 cut(s)	202, 514, 612
SduI	GDGCHC	2 cut(s)	71, 835
SfaNI	GCATC	5 cut(s)	340, 610, 643, 992, 1045
SfcI	CTRYAG	2 cut(s)	548, 854
SfoI	GGCGCC	1 cut(s)	244
SfuI	TTCGAA	1 cut(s)	83
SgrAI	CRCCGGYG	1 cut(s)	239
SinI	GGWCC	4 cut(s)	97, 712, 784, 1070
SmlI	CTYRAG	1 cut(s)	212
SmoI	CTYRAG	1 cut(s)	212
SphI	GCATGC	1 cut(s)	329
Sse9I	AATT	6 cut(s)	339, 368, 396, 447, 938, 1002
SsiI	CCGC	9 cut(s)	51, 111, 149, 155, 161, 171, 174, 249, 485
SspDI	GGCGCC	1 cut(s)	242
SspMI	CTAG	3 cut(s)	192, 438, 740
SstI	GAGCTC	1 cut(s)	71
StyD4I	CCNGG	3 cut(s)	200, 512, 610
TaiI	ACGT	1 cut(s)	78
TaqI	TCGA	6 cut(s)	44, 83, 281, 336, 463, 1075
TasI	AATT	6 cut(s)	339, 368, 396, 447, 938, 1002
TatI	WGTACW	1 cut(s)	216
TauI	GCSGC	3 cut(s)	113, 173, 176
TfiI	GAWTC	1 cut(s)	732
TseI	GCWGC	4 cut(s)	176, 416, 749, 1063
TspDTI	ATGAA	6 cut(s)	303, 666, 913, 1005, 1006, 1014
TspGWI	ACGGA	1 cut(s)	244
VpaK11BI	GGWCC	4 cut(s)	97, 712, 784, 1070
XapI	RAATTY	4 cut(s)	339, 368, 938, 1002
XceI	RCATGY	1 cut(s)	329
XmiI	GTMKAC	1 cut(s)	356
XmnI	GAANNNNTTC	2 cut(s)	459, 492
XspI	CTAG	3 cut(s)	192, 438, 740
ZraI	GACGTC	1 cut(s)	76
ZrmI	AGTACT	1 cut(s)	218

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.