Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0010474

Physical Location & Seq

Genomic Coordinates Forward (+)

29183650 .. 29187863

Gene Structure 4214 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1201300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1086 bp

ATGTCCTCGATACTGCTTCGGTCCCGTTCAGTCTCCTGGCTGTTAAAGCGCAATCGACGCCTATTGAGCTCTGATGTGGCTTCAAACCCTTCCAAAGAACCTCTCATCGGAGCCCAATCCCTAGTCTCCGATCTCACATCTCCACCTCCACCGCCACCACAAAGCACTCCCGCTCCGAACGCTTCAAGTAGCAAATCTTGGAACTTTCTCAAATACTCGCTCATTGGCGCGATCACTGGAGCCACTGCTTCTGTTGGTTATGTCTCGTATGCATACTCTTTGGATGAAATCGATGAAAAGACCAAGGCTCTCCGAGAAGCACCTTCAACTATCAAGTTGGCAGATGATGCATCCGCTCTTGAAAAATTTAAAAATTGGGTATACTCTTCTGCAGTTACAGTGCCTGCTAAAGCAGCTGAAGCTTACATTGATGCAAGGAGGACGATTGAAGAACAAATTCGAGGTTATACTGAACCATATGCAGAGAAGCTTCTCCCAGATTTGCATCCCATGGAGAAACATGTTTTTACCCTTGTTCTGGATCTCCAGGAGACATTACTGTACTCTTATTGGACGCGAGAAAAGGGCTGGCAGACTATCAAAAGACCTGGAGTTGATGCCTTCTTGGAACATCTTGCTCAGTTTTATGAAATCATTGTGTATTCAGACTATTCAAATATGTATGTAGATCCTGTAATGGAGAGACTGGACACCAAGAATTGTGTAAGATATAGGCTGGGAAAGGCTGCTACTAAGTATCAGAATGGACAACATTATAGGGACCTTTCAAAACTTAACAGAGATCCAAAAAAGATCATCTATCTGAGTGCCCATGCACGAGAAAATAGTCTGCAGCCCGAAAATGGTGCCATAATAAAGCCATATAAGTATGAGTTGGATGACACAGCTCTTGTGGATTTCATACCATTTCTTGAATTTGTTGCCCGTAATCCTCCAGCAGATATCAGGCAAGTATTAGAATCATATGAAGGGCGTGATATTCCTGCAGAGTTCATTAGGCGATCTAAAGAGCATCAACAGCGGATGCAAAATCAAAAGCAGCAGGCTCGATTATGGAGACGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005743 GO:0005744 GO:0006464 GO:0006470 GO:0006605 GO:0006626 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006839 GO:0006886 GO:0006996 GO:0007005 GO:0008104 GO:0008150 GO:0008152 GO:0009987 GO:0015031 GO:0015833 GO:0016020 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0017038 GO:0019538 GO:0019866 GO:0030150 GO:0031090 GO:0031966 GO:0031967 GO:0031975 GO:0032991 GO:0033036 GO:0033365 GO:0034613 GO:0036211 GO:0042578 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0044743 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0065002 GO:0070585 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0071840 GO:0072594 GO:0072655 GO:0098796 GO:0098798 GO:0098800 GO:0140096 GO:1901564 GO:1990542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NIF

Protein Analysis

362

Amino Acids

41.38

Weight (kDa)

9.04

Isoelectric Point (pI)

43.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NIF	PF03031	177 - 316	9.6e-35	NLI interacting factor-like phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010474)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55900 AT1G55900
fragaria_vesca	FvH4_3g29430 FvH4_3g29430
malus_domestica	MD03G1185000.v1.1 MD11G1201300.v1.1
prunus_persica	Prupe.4G241500_v2.0.a1 Prupe.4G241500_v2.0.a1 Prupe.4G241500_v2.0.a1
pyrus_communis	pycom03g13810
rosa_chinensis	RchiOBHm_Chr5g0053871
rosa_laevigata	RLG00000034907
rosa_roxburghii	Rroxscaffold_1G00026740
rosa_rugosa	Rorug05G0283800 Rorug05G0283900
rosa_samantha	Rh5AG353600 Rh5BG365200 Rh5CG390300 Rh5DG380100
rosa_wichuraiana	Rw0G002120 Rw0G009530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	866
AccBSI	CCGCTC	2 cut(s)	173, 356
AccI	GTMKAC	1 cut(s)	381
AccII	CGCG	2 cut(s)	230, 577
AciI	CCGC	4 cut(s)	152, 171, 354, 1042
AclWI	GGATC	3 cut(s)	549, 683, 797
AcsI	RAATTY	3 cut(s)	365, 456, 935
AcuI	CTGAAG	1 cut(s)	438
AcyI	GRCGYC	1 cut(s)	58
AfaI	GTAC	1 cut(s)	563
AfiI	CCNNNNNNNGG	3 cut(s)	107, 538, 863
AflIII	ACRYGT	1 cut(s)	520
AgsI	TTSAA	8 cut(s)	84, 186, 327, 362, 449, 675, 789, 935
AjnI	CCWGG	3 cut(s)	35, 546, 607
AluBI	AGCT	5 cut(s)	69, 416, 422, 490, 908
AluI	AGCT	5 cut(s)	69, 416, 422, 490, 908
Alw21I	GWGCWC	1 cut(s)	71
Alw26I	GTCTC	6 cut(s)	37, 130, 268, 545, 697, 1072
AlwI	GGATC	3 cut(s)	549, 683, 797
AlwNI	CAGNNNCTG	1 cut(s)	404
ApeKI	GCWGC	4 cut(s)	413, 746, 853, 1060
ApoI	RAATTY	3 cut(s)	365, 456, 935
Asp700I	GAANNNNTTC	1 cut(s)	456
AspLEI	GCGC	2 cut(s)	51, 230
AspS9I	GGNCC	2 cut(s)	21, 781
AvaII	GGWCC	2 cut(s)	21, 781
BaeGI	GKGCMC	1 cut(s)	832
BanI	GGYRCC	1 cut(s)	866
BanII	GRGCYC	2 cut(s)	71, 115
BauI	CACGAG	1 cut(s)	837
Bbv12I	GWGCWC	1 cut(s)	71
BbvI	GCAGC	4 cut(s)	425, 733, 865, 1072
BcgI	CGANNNNNNTGC	4 cut(s)	848, 882, 1049, 1083
BciT130I	CCWGG	3 cut(s)	37, 548, 609
BcoDI	GTCTC	6 cut(s)	37, 130, 268, 545, 697, 1072
BfaI	CTAG	1 cut(s)	122
BfmI	CTRYAG	3 cut(s)	390, 851, 1005
BisI	GCNGC	4 cut(s)	414, 747, 854, 1061
BlsI	GCNGC	4 cut(s)	415, 748, 855, 1062
Bme1390I	CCNGG	3 cut(s)	37, 548, 609
Bme18I	GGWCC	2 cut(s)	21, 781
BmgT120I	GGNCC	2 cut(s)	21, 781
BmiI	GGNNCC	5 cut(s)	23, 112, 241, 782, 868
BmrFI	CCNGG	3 cut(s)	37, 548, 609
BmsI	GCATC	7 cut(s)	337, 359, 421, 514, 607, 1035, 1042
BpmI	CTGGAG	4 cut(s)	258, 530, 630, 939
Bsa29I	ATCGAT	1 cut(s)	291
BsaBI	GATNNNNATC	1 cut(s)	504
BsaHI	GRCGYC	1 cut(s)	58
BsaJI	CCNNGG	2 cut(s)	303, 510
BsaXI	ACNNNNNCTCC	2 cut(s)	157, 187
Bsc4I	CCNNNNNNNGG	3 cut(s)	107, 538, 863
Bse1I	ACTGG	2 cut(s)	241, 711
Bse8I	GATNNNNATC	1 cut(s)	504
BseBI	CCWGG	3 cut(s)	37, 548, 609
BseCI	ATCGAT	1 cut(s)	291
BseDI	CCNNGG	2 cut(s)	303, 510
BseGI	GGATG	5 cut(s)	289, 350, 505, 904, 1050
BseJI	GATNNNNATC	1 cut(s)	504
BseLI	CCNNNNNNNGG	3 cut(s)	107, 538, 863
BseMII	CTCAG	2 cut(s)	653, 815
BseNI	ACTGG	2 cut(s)	241, 711
BseSI	GKGCMC	1 cut(s)	832
BseXI	GCAGC	4 cut(s)	425, 733, 865, 1072
BseYI	CCCAGC	1 cut(s)	736
Bsh1236I	CGCG	2 cut(s)	230, 577
BshNI	GGYRCC	1 cut(s)	866
BshVI	ATCGAT	1 cut(s)	291
BsiHKAI	GWGCWC	1 cut(s)	71
BslFI	GGGAC	2 cut(s)	7, 794
BslI	CCNNNNNNNGG	3 cut(s)	107, 538, 863
BsmAI	GTCTC	6 cut(s)	37, 130, 268, 545, 697, 1072
BsmBI	CGTCTC	1 cut(s)	1072
BsmFI	GGGAC	2 cut(s)	7, 794
Bsp1286I	GDGCHC	3 cut(s)	71, 115, 832
Bsp143I	GATC	7 cut(s)	130, 231, 541, 688, 802, 813, 1022
Bsp19I	CCATGG	1 cut(s)	510
BspACI	CCGC	4 cut(s)	152, 171, 354, 1042
BspCNI	CTCAG	2 cut(s)	652, 816
BspDI	ATCGAT	1 cut(s)	291
BspFNI	CGCG	2 cut(s)	230, 577
BspLI	GGNNCC	5 cut(s)	23, 112, 241, 782, 868
BspMAI	CTGCAG	3 cut(s)	394, 855, 1009
BspPI	GGATC	3 cut(s)	549, 683, 797
BspT107I	GGYRCC	1 cut(s)	866
BsrBI	CCGCTC	2 cut(s)	173, 356
BsrI	ACTGG	2 cut(s)	241, 711
BssECI	CCNNGG	2 cut(s)	303, 510
BssMI	GATC	7 cut(s)	130, 231, 541, 688, 802, 813, 1022
BssNAI	GTATAC	1 cut(s)	382
BssNI	GRCGYC	1 cut(s)	58
BssSI	CACGAG	1 cut(s)	837
BssT1I	CCWWGG	2 cut(s)	303, 510
Bst1107I	GTATAC	1 cut(s)	382
Bst2BI	CACGAG	1 cut(s)	837
Bst2UI	CCWGG	3 cut(s)	37, 548, 609
Bst4CI	ACNGT	2 cut(s)	400, 561
Bst6I	CTCTTC	1 cut(s)	391
BstACI	GRCGYC	1 cut(s)	58
BstAPI	GCANNNNNTGC	1 cut(s)	347
BstC8I	GCNNGC	3 cut(s)	405, 590, 1065
BstDEI	CTNAG	3 cut(s)	639, 753, 824
BstDSI	CCRYGG	1 cut(s)	510
BstF5I	GGATG	5 cut(s)	289, 350, 505, 904, 1050
BstFNI	CGCG	2 cut(s)	230, 577
BstHHI	GCGC	2 cut(s)	51, 230
BstKTI	GATC	7 cut(s)	133, 234, 544, 691, 805, 816, 1025
BstMAI	GTCTC	6 cut(s)	37, 130, 268, 545, 697, 1072
BstMBI	GATC	7 cut(s)	130, 231, 541, 688, 802, 813, 1022
BstMWI	GCNNNNNNNGC	8 cut(s)	46, 57, 66, 179, 347, 413, 419, 1039
BstNI	CCWGG	3 cut(s)	37, 548, 609
BstNSI	RCATGY	1 cut(s)	524
BstSCI	CCNGG	3 cut(s)	35, 546, 607
BstSFI	CTRYAG	3 cut(s)	390, 851, 1005
BstSLI	GKGCMC	1 cut(s)	832
BstUI	CGCG	2 cut(s)	230, 577
BstV1I	GCAGC	4 cut(s)	425, 733, 865, 1072
BstX2I	RGATCY	3 cut(s)	541, 688, 802
BstYI	RGATCY	3 cut(s)	541, 688, 802
BstZ17I	GTATAC	1 cut(s)	382
Bsu15I	ATCGAT	1 cut(s)	291
BsuTUI	ATCGAT	1 cut(s)	291
BtgI	CCRYGG	1 cut(s)	510
BtsCI	GGATG	5 cut(s)	289, 350, 505, 904, 1050
BtsI	GCAGTG	1 cut(s)	243
BtsIMutI	CAGTG	3 cut(s)	234, 243, 405
Cac8I	GCNNGC	3 cut(s)	405, 590, 1065
CaiI	CAGNNNCTG	1 cut(s)	404
CfoI	GCGC	2 cut(s)	51, 230
Cfr13I	GGNCC	2 cut(s)	21, 781
ClaI	ATCGAT	1 cut(s)	291
CseI	GACGC	2 cut(s)	66, 583
Csp6I	GTAC	1 cut(s)	562
CviAII	CATG	3 cut(s)	511, 521, 833
CviQI	GTAC	1 cut(s)	562
DdeI	CTNAG	3 cut(s)	639, 753, 824
DpnI	GATC	7 cut(s)	132, 233, 543, 690, 804, 815, 1024
DpnII	GATC	7 cut(s)	130, 231, 541, 688, 802, 813, 1022
DraI	TTTAAA	1 cut(s)	370
Eam1104I	CTCTTC	1 cut(s)	391
EarI	CTCTTC	1 cut(s)	391
Ecl136II	GAGCTC	1 cut(s)	69
Eco130I	CCWWGG	2 cut(s)	303, 510
Eco24I	GRGCYC	2 cut(s)	71, 115
Eco32I	GATATC	1 cut(s)	964
Eco47I	GGWCC	2 cut(s)	21, 781
Eco53kI	GAGCTC	1 cut(s)	69
Eco57I	CTGAAG	1 cut(s)	438
EcoICRI	GAGCTC	1 cut(s)	69
EcoO109I	RGGNCCY	1 cut(s)	781
EcoRII	CCWGG	3 cut(s)	35, 546, 607
EcoRV	GATATC	1 cut(s)	964
EcoT14I	CCWWGG	2 cut(s)	303, 510
EcoT22I	ATGCAT	2 cut(s)	274, 352
EcoT38I	GRGCYC	2 cut(s)	71, 115
ErhI	CCWWGG	2 cut(s)	303, 510
Esp3I	CGTCTC	1 cut(s)	1072
FaeI	CATG	3 cut(s)	514, 524, 836
FaqI	GGGAC	2 cut(s)	7, 794
FatI	CATG	3 cut(s)	510, 520, 832
FauI	CCCGC	1 cut(s)	178
FauNDI	CATATG	2 cut(s)	478, 985
FblI	GTMKAC	1 cut(s)	381
Fnu4HI	GCNGC	4 cut(s)	414, 747, 854, 1061
FokI	GGATG	5 cut(s)	296, 337, 492, 911, 1057
FriOI	GRGCYC	2 cut(s)	71, 115
Fsp4HI	GCNGC	4 cut(s)	414, 747, 854, 1061
FspBI	CTAG	1 cut(s)	122
GlaI	GCGC	2 cut(s)	50, 229
GluI	GCNGC	4 cut(s)	414, 747, 854, 1061
GsaI	CCCAGC	1 cut(s)	740
GsuI	CTGGAG	4 cut(s)	258, 530, 630, 939
HgaI	GACGC	2 cut(s)	66, 583
HhaI	GCGC	2 cut(s)	51, 230
Hin1I	GRCGYC	1 cut(s)	58
Hin1II	CATG	3 cut(s)	514, 524, 836
Hin6I	GCGC	2 cut(s)	49, 228
HinP1I	GCGC	2 cut(s)	49, 228
HindIII	AAGCTT	2 cut(s)	420, 488
HinfI	GANTC	1 cut(s)	980
Hpy166II	GTNNAC	1 cut(s)	382
Hpy188I	TCNGA	8 cut(s)	73, 110, 130, 177, 314, 667, 762, 825
Hpy188III	TCNNGA	3 cut(s)	359, 539, 932
Hpy8I	GTNNAC	1 cut(s)	382
Hpy99I	CGWCG	1 cut(s)	60
HpyAV	CCTTC	4 cut(s)	99, 333, 631, 983
HpyCH4III	ACNGT	2 cut(s)	400, 561
HpyCH4IV	ACGT	1 cut(s)	1081
HpyF10VI	GCNNNNNNNGC	8 cut(s)	46, 57, 66, 179, 347, 413, 419, 1039
HpyF3I	CTNAG	3 cut(s)	639, 753, 824
HpySE526I	ACGT	1 cut(s)	1081
Hsp92I	GRCGYC	1 cut(s)	58
Hsp92II	CATG	3 cut(s)	514, 524, 836
HspAI	GCGC	2 cut(s)	49, 228
Kzo9I	GATC	7 cut(s)	130, 231, 541, 688, 802, 813, 1022
LmnI	GCTCC	3 cut(s)	110, 178, 239
Lsp1109I	GCAGC	4 cut(s)	425, 733, 865, 1072
LweI	GCATC	7 cut(s)	337, 359, 421, 514, 607, 1035, 1042
MaeI	CTAG	1 cut(s)	122
MaeII	ACGT	1 cut(s)	1081
MaeIII	GTNAC	1 cut(s)	394
MalI	GATC	7 cut(s)	132, 233, 543, 690, 804, 815, 1024
MbiI	CCGCTC	2 cut(s)	173, 356
MboI	GATC	7 cut(s)	130, 231, 541, 688, 802, 813, 1022
MboII	GAAGA	2 cut(s)	378, 461
MflI	RGATCY	3 cut(s)	541, 688, 802
MhlI	GDGCHC	3 cut(s)	71, 115, 832
MluCI	AATT	5 cut(s)	365, 373, 456, 718, 935
MmeI	TCCRAC	1 cut(s)	876
MnlI	CCTC	6 cut(s)	16, 111, 156, 432, 455, 963
Mph1103I	ATGCAT	2 cut(s)	274, 352
MroXI	GAANNNNTTC	1 cut(s)	456
MseI	TTAA	3 cut(s)	44, 369, 795
MspA1I	CMGCKG	2 cut(s)	416, 1042
MspR9I	CCNGG	3 cut(s)	37, 548, 609
MvaI	CCWGG	3 cut(s)	37, 548, 609
MvnI	CGCG	2 cut(s)	230, 577
MwoI	GCNNNNNNNGC	8 cut(s)	46, 57, 66, 179, 347, 413, 419, 1039
NcoI	CCATGG	1 cut(s)	510
NdeI	CATATG	2 cut(s)	478, 985
NdeII	GATC	7 cut(s)	130, 231, 541, 688, 802, 813, 1022
NlaIII	CATG	3 cut(s)	514, 524, 836
NlaIV	GGNNCC	5 cut(s)	23, 112, 241, 782, 868
NsiI	ATGCAT	2 cut(s)	274, 352
NspI	RCATGY	1 cut(s)	524
PciI	ACATGT	1 cut(s)	520
PdmI	GAANNNNTTC	1 cut(s)	456
PfeI	GAWTC	1 cut(s)	980
PfoI	TCCNGGA	1 cut(s)	546
PkrI	GCNGC	4 cut(s)	415, 748, 855, 1062
PpuMI	RGGWCCY	1 cut(s)	781
PscI	ACATGT	1 cut(s)	520
Psp124BI	GAGCTC	1 cut(s)	71
Psp5II	RGGWCCY	1 cut(s)	781
Psp6I	CCWGG	3 cut(s)	35, 546, 607
PspFI	CCCAGC	1 cut(s)	736
PspGI	CCWGG	3 cut(s)	35, 546, 607
PspN4I	GGNNCC	5 cut(s)	23, 112, 241, 782, 868
PspPI	GGNCC	2 cut(s)	21, 781
PspPPI	RGGWCCY	1 cut(s)	781
PstI	CTGCAG	3 cut(s)	394, 855, 1009
PstNI	CAGNNNCTG	1 cut(s)	404
PsuI	RGATCY	3 cut(s)	541, 688, 802
PvuII	CAGCTG	1 cut(s)	416
RsaI	GTAC	1 cut(s)	563
RsaNI	GTAC	1 cut(s)	562
SacI	GAGCTC	1 cut(s)	71
SaqAI	TTAA	3 cut(s)	44, 369, 795
SatI	GCNGC	4 cut(s)	414, 747, 854, 1061
Sau3AI	GATC	7 cut(s)	130, 231, 541, 688, 802, 813, 1022
Sau96I	GGNCC	2 cut(s)	21, 781
ScrFI	CCNGG	3 cut(s)	37, 548, 609
SduI	GDGCHC	3 cut(s)	71, 115, 832
SfaNI	GCATC	7 cut(s)	337, 359, 421, 514, 607, 1035, 1042
SfcI	CTRYAG	3 cut(s)	390, 851, 1005
SinI	GGWCC	2 cut(s)	21, 781
Sse9I	AATT	5 cut(s)	365, 373, 456, 718, 935
SsiI	CCGC	4 cut(s)	152, 171, 354, 1042
SspMI	CTAG	1 cut(s)	122
SstI	GAGCTC	1 cut(s)	71
StyD4I	CCNGG	3 cut(s)	35, 546, 607
StyI	CCWWGG	2 cut(s)	303, 510
TaaI	ACNGT	2 cut(s)	400, 561
TaiI	ACGT	1 cut(s)	1084
TaqI	TCGA	5 cut(s)	8, 55, 291, 460, 1069
TaqII	GACCGA	1 cut(s)	9
TasI	AATT	5 cut(s)	365, 373, 456, 718, 935
TatI	WGTACW	1 cut(s)	561
TfiI	GAWTC	1 cut(s)	980
Tru1I	TTAA	3 cut(s)	44, 369, 795
Tru9I	TTAA	3 cut(s)	44, 369, 795
TscAI	CASTG	3 cut(s)	241, 250, 405
TseI	GCWGC	4 cut(s)	413, 746, 853, 1060
TspDTI	ATGAA	6 cut(s)	300, 309, 663, 910, 1002, 1003
TspRI	CASTG	3 cut(s)	241, 250, 405
VpaK11BI	GGWCC	2 cut(s)	21, 781
XapI	RAATTY	3 cut(s)	365, 456, 935
XceI	RCATGY	1 cut(s)	524
XmiI	GTMKAC	1 cut(s)	381
XmnI	GAANNNNTTC	1 cut(s)	456
XspI	CTAG	1 cut(s)	122
Zsp2I	ATGCAT	2 cut(s)	274, 352

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.